420results about How to "Improve hydrolysis effect" patented technology

The invention provides a method for producing high-quality collagen oligopeptide by using fish skin or fishbone. The method comprises the following steps of: washing and crushing the fish skin or fishbone as a raw material, mixing the crushed material and water according to a weight ratio of 1:(5-20), carrying out refining treatment, then sterilizing and cooling, simultaneously adding proteolytic enzyme and lactobacillus to carry out primary hydrolysis, then centrifuging and filtering to separate a non-hydrolyzed solid part and hydrolysate, carrying out ultrafiltration on the hydrolysate by adopting an ultrafiltration film, mixing a macromolecular peptide solution which does not pass through the ultrafiltration film and the non-hydrolyzed solid part which is separated after the primary hydrolysis is finished, simultaneously adding protease and lactobacillus to carry out secondary hydrolysis, carrying out centrifugal separation and filtration, then carrying out ultrafiltration, and sequentially purifying, sterilizing, condensing and drying a micromolecular oligopeptide solution which passes through the ultrafiltration film and micromolecular oligopeptide collected after the primary hydrolysis to obtain the collagen oligopeptide product with low molecular weight. According to the method provided by the invention, the utilization rate and the yield of the raw material protein can be effectively improved, and the product quality can be greatly improved.

The invention discloses a method for preparing a bio-carrier by porous foam polymer modification. The method is used for coating bonding modification of porous foam polymers. The method comprises the following steps of adding one or more cationized porous high-adsorptivity functional materials and one or more cationized polyhydric hydrophilic functional materials into coating modification slurry, mixing the coating modification slurry and a cationic acrylic acid emulsion, carrying out coating bonding of the mixture on one or more porous foam polymers, and carrying out cross-linking curing to obtain the modified bio-carrier having strong adsorbability, hydrophily, surface active functional group diversity, positive surface charges and strong biocompatibility. The method provided by the invention is not limited by structures, shapes, aperture sizes and physical properties of foam polymers and can be widely used for external coating modification of multiple carrier materials. The method provided by the invention has the advantages of convenient and simple operation, low investment and making cost, excellent carrier performances and long service life. The bio-carrier obtained by the method can be widely used in fields of fixed attachment of various microorganisms, and enhanced biological treatment.

The invention relates to an extraction method of an active substrate, and in particular relates to a method for extracting an active substance from the sea cucumber processing waste. By taking the viscera of sea cucumber as a raw material, the method is used for preparing refined sea cucumber polysaccharide through the processes of pretreatment, enzymolysis, reflux extraction, microwave extraction, alcohol precipitation, decoloration, centrifugation, salt precipitation, dialysis, refining and the like; the supernate after alcohol precipitation is subjected to the processes of rotary evaporation, extraction degreasing, water saturation n-butyl alcohol extraction, rotary evaporation, macroporous adsorption resin chromatography and the like to obtain refined sea cucumber total saponins; and the aqueous solution after the water saturation n-butyl alcohol extraction is processed by the technologies of decoloration, sterilization and the like to obtain antioxidant active sea cucumber polypeptide. The method provided by the invention turns the waste of sea cucumber viscera into wealth so as to obtain three products including sea cucumber polysaccharide, sea cucumber total saponins and antioxidant active sea cucumber polypeptide.

The invention discloses an angiotensin converting enzyme inhibitory peptide, which is characterized by comprising the following amino acid sequences: SEQ NO1: Arg-Leu-Ser-Phe-Asn-Pro, SEQ NO2: Leu-Leu, and SEQ NO3: Ala-Leu-Pro-Met-His-Ile-Arg. The method for preparing the inhibitory peptides comprises the following steps of: (1) preparing lactalbumin concentrated solution; (2) preparing lactalbumin hydrolysis liquid; (3) preparing angiotensin converting enzyme-containing inhibitory peptide concentrated solution; (4) performing separation and purification on the inhibitory peptide-containing concentrated solution through gel chromatography and reverse-phase high-pressure liquid chromatography; and (5) obtaining the product by freeze drying. The invention has the advantages that: three amino acid sequences of the angiotensin converting enzyme inhibitory peptide derived from the lactalbumin are disclosed for the first time, the preparation cost is low and the extraction ratio is high.

The present invention relates to the field of nucleic acid extraction, particularly to a nucleic acid extraction method and a nucleic acid extraction reagent for biological samples. The specific method comprises: adding a sodium hydroxide solution to a biological sample, adding a lysate, and carrying out lysis to obtain a lysis reaction liquid, wherein the lysate contains a guanidine salt, disodium edetate, a surfactant and a buffer agent; and adding an ethanol solution to the lysis reaction liquid, and achieving nucleic acid adsorption, cleaning and elution by using an instrument-free nucleic acid extraction apparatus having a silica gel membrane or a centrifugal column. According to the present invention, the sodium hydroxide is added to pre-treat and then the lysate is added to carry out lysis, such that the protein in the sample can be well dissolved, and the binding of the nucleic acid and the adsorption film is prompted; and the lysate component is optimized, such that the binding capacity of the nucleic acid molecule and the silica gel membrane under the high salt and high pH value conditions is significantly enhanced, and the good nucleic acid extraction efficiency is ensured.

The invention discloses a low-temperature reactive dye soaping agent as well as a preparation method and an application thereof. The low-temperature reactive dye soaping agent comprises the following components in percentage by mass: 10%-20% of a maleic-methyl acrylate copolymer, 2%-5% of an alkaline substance, 10%-20% of an oxidant and the balance of water.

The invention provides a beta-glucosidase as well as a preparation method and an application thereof. The amino acid sequence of beta-glucosidase is shown as SEQ ID NO.1. The beta-glucosidase has excellent thermal stability, can resist high temperature, has higher beta-1,6-glucosidic bond hydrolysis capacity as well as higher alpha-1,6-arabinopyranoside bond hydrolysis capacity and has higher transformation capacity for ginsenoside Rb1 and Rb2. After the beta-glucosidase is incubated with the ginsenoside Rb1 and Rb2 for certain period, ginsenoside Rb1 and Rb2 are almost transformed into ginsenoside Rd completely. According to a preparation method of TPEBGL1, high-efficiency expression can be realized without an inducer IPTG (isopropyl beta-D-1-thiogalactopyranoside).

The invention provides a method for producing beer malt extract comprising the steps of screening barley raw material, unhusking the barley, disintegrating barley granules into powder form, charging water, calcium chloride, neutral proteinase and beta-dextranase, heat preserving 1-10 hours at 45-55 deg. C, heat preserving 1-10 hours at 55-68 deg. C, heat preserving 1-10 hours at 68-78 deg. C, thus completing grout conditioning, charging high temperature resistant alpha-amylase into slip for liquefaction, charging into slip by amylase, neutral proteinase and beta-dextranase, stewing 10-36 hours, elevating the temperature to 60-70 deg. C, stewing 1-10 hours, elevating the temperature to 75-80 deg. C, heat preserving 1-2 hours, thus finishing saccharifying, then filtering and condensing.

The invention relates to a spinning straw fiber extraction method. The spinning rice and wheat straw fiber extraction method is characterized in that the method includes the following steps: 1). the steam explosion pretreatment: straws are air-dried and then are cut into segments; 2). the pretreated straws are put in a steam blasting tank; 3). nutrient glucose and urea are recharged; 4). the strain degumming: first, choosing strains: choosing mushroom white rot fungi or planerochaete chrysosporium as the strain; second, to pave the steam explosion straw mixture obtained in the third step to be 10-100mm high; third, poking the strains onto the straw piles; fourth, culturing on the culturing bed; fifth, cleaning; sixth, bleaching; 5). irradiation curing: the bleached straw fiber is irradiated with Gamma rays with the irradiation amount of 2-10kgy to obtain the radiation curing straw fiber; 6). the straw fiber is oiled; 7) the straw fiber is dried; 8) the dried straw fiber is dispersed to obtain the spinning rice and wheat straw fiber. The invention has the characteristics of environmental protection and low cost.

A provided preparation method for wool keratin regenerated cellulose fiber comprises the concrete steps: firstly preparing a viscose-rayon spinning solution, then preparing a wool keratin solution, then mixing the viscose-rayon spinning solution and the wool keratin solution, then adding a crosslinking agent, mixing and performing wet-process spinning, so as to obtain wool keratin regenerated cellulose fiber. The wool keratin regenerated cellulose fiber prepared by employing the method has the protein content of 6% or more, and the production technology is simple in process and high in production efficiency.

The invention discloses a method for making a crab nutritional instant solid beverage, which is characterized by comprising the following steps of grinding portunid leftovers, drying, degreasing, carrying out composite enzymolysis, centrifuging, concentrating clear liquid, seasoning, homogenizing, spraying, drying, etc., wherein composite enzymolysis adopts neutral protease and flavored protease. The method effectively extracts nutritional components in the portunid leftover to product the solid beverage, which changes waste into valuables, produces considerable economic benefit, can satisfy the consuming needs of people, and can promote human health.

The invention relates to a method for extracting a euphorbia peplus plant extract for preparing an antitumor medicament from a euphorbia peplus plant. The method comprises the following process steps of: smashing raw materials; performing reflux extraction; concentrating under reduced pressure; extracting; performing back-extraction; performing alkaline hydrolysis; adjusting PH value to 7; concentrating under reduced pressure; performing silica gel column chromatography; performing gradient elution with petroleum ether and ethyl acetate; concentrating; crystallizing; and drying. The method has the advantages of capability of realizing industrial production scale because industrially readily-available separating materials and reagents are adopted, high product quality, content of over 99.9percent and strong international market competitiveness.

The invention relates to a production method for a nonwoven material, in particular to raw paper for dispersed wet tissues and a production method for the raw paper. The raw paper for the dispersed wet tissues is a nonwoven material obtained by sequentially executing a papermaking procedure, a spunlace entanglement procedure and a dehydration drying procedure on papermaking pulp consisting of 25 to 28 weight percent of polylactic acid fibers, 40 to 42 weight percent of coconut coal fibers and the balance of wood pulp fibers, and does not contain any additional bonding agent and wet strength agent. According to the raw paper for the dispersed wet tissues and the production method, a gap in a production technology for the raw paper for the dispersed wet tissues in China is supplied, foreign and domestic market requirements are met, and a conventional raw paper production process is simplified; papermaking is combined with spunlace, so that the production cost is lowered, and the wet strength, wet wiping effects and hydrolytic property of the raw paper for the dispersed wet tissues are importantly improved; an obtained product is high in dispersibility and biodegradability.

The invention discloses a method for determining the form of selenium in selenium-enriched edible fungi. The method is characterized by comprising the following steps of: (1) pretreating selenium-enriched edible fungi: using deionized water as an extracting solution to extract a crushed selenium-enriched edible fungus sample, then adding effective amounts of protease K and protease XIV for hydrolysis so as to extract a sample solution to be detected; (2) conducting hydride generation: combining atomic fluorescence spectrometry with high performance liquid chromatography to determine a standard selenium form solution and the sample solution to be detected respectively. The method of the invention has the advantages of simplicity and easy operation, good repeatability, and employment of non-toxic and pollution-free reagents.

The invention discloses a preparation process of red date condensed juice high in cAMP content. The preparation process includes the following steps of firstly, conducting separating, washing and smashing; secondly, conducting hot water extraction; thirdly, conducting cooling and heat preservation enzymic method oriented conversion; fourthly, adding composite enzyme preparations; fifthly, inactivating enzymes; sixthly, conducting centrifugation; seventhly, conducting filtration; eighthly, conducting concentration and pasteurization; ninthly, conducting filling. Compared with the prior art, the preparation process has the advantages that the process is simple, the production period is shortened, and the energy loss is reduced; extraction is conducted after smashing is conducted, and therefore extraction of nutritional ingredients in red dates can be improved; by means of the extraction and enzymolysis combined method, the juice yield is greatly improved, the processing time is greatly shortened, and the cyclic adenosine monophosphate content in red date juice is improved; date peel is utilized, and the cAMP content is improved; preparations of pectinase, cellulose and protease are used in a composite mode, and the hydrolysis capacity of enzymic preparations is improved; the step-by-step enzymolysis and extraction process is adopted, and interference between related enzyme preparations is avoided.

A two-component curable adhesive or sealant composition is provided. The first component may comprise a mixture of at least one polyol selected from the group comprising a polyester polyol, a polyester-polycarbonate copolymer polyol, and combinations thereof, a resin, and optionally a solvent. The second component may comprise a prepolymer obtained by reacting a polyester-polycarbonate copolymer polyol which is the reaction product of a polyester polyol which is the reaction product of one or more organic acids, and one or more glycols having a functionality of two or more and one or more polycarbonate polyols, at least one organic polyisocyanate component, and at least one chain extending agent and optionally a solvent. Alternatively, the first component may comprise a polyester-polycarbonate copolymer polyol, a resin, and optionally a solvent. The second component may comprise a polyisocyanate curative and optionally a solvent. The cured adhesive exhibits improved hydrolytic properties while maintaining excellent processability and adhesive properties.

The invention discloses deer blood wine and a preparation method of the deer blood wine and belongs to the technical field of nutritional Baijiu. Fresh deer blood and deer whips are used as main raw materials and are scientifically compounded with lycium ruthenicum, fructus lycii, mulberry fruit, fructus jujubae, longan, liquorice root and the like; a whole process adopts low-temperature processing technologies of freeze-drying, carrying out biological enzymolysis by a complex enzyme, degassing and deodorizing in vacuum, freezing, carrying out low-temperature fermentation by functional saccharomyces cerevisiae, carrying out ultrasonic processing, carrying out high-voltage pulse electric field processing and the like; the utilization rate of effective components of deer products is effectively improved, and the loss of nutrients and health-care substances is reduced; the original color and luster, flavor and the content of bioactive components and nutrient substances of the deer products are kept to the greatest extent. Finally, the deer blood wine which has no odor, high stability, coordinated taste, mellowness, softness, long aftertaste, moderate flavor, high bioactive components and nutrient value and good health-care effect and is clarified and transparent is prepared.

The invention relates to a method for producing sugars, such as glucose, by fractionating lignocellulose-containing biomass. The sugar product thus obtained is useful for the manufacture of bioethanol and other chemicals.

Ring-opening polymerization of a cyclic ester is performed by using an alcohol and water positively as initiators or/and molecular weight-adjusting agents to control the initial properties and properties with time of the resultant aliphatic polyester. More specifically, an aliphatic polyester is produced by ring-opening polymerization of a cyclic ester containing water and an alcohol based on a total proton concentration and a ratio between a mol concentration of carboxyl (carboxylic acid)-source compounds including water and a mol concentration of alkoxycarbonyl (ester)-source compounds, as polymerization-controlling indexes.

The invention relates to a biomass pretreatment method which can be a substitute for alkali, acid and steam explosion and reduce the cost and environmental pollution. The invention adopts the technical scheme that: crushing the biomass into powder, sterilizing, cooling, then adding proteinase, hydrolyzing protein in the biomass into free amino acids, and then using other methods to hydrolyze cellulose and hemicellulose into monose for alcoholic fermentation. The proteinase comprises acid protease, alkali protease or collagenase and the like. The biomass comprises crop straws, grass, wood or fibrous plants.