49results about How to "Reduce agglutination" patented technology

The present invention relates to the field of nucleic acid extraction, particularly to a nucleic acid extraction method and a nucleic acid extraction reagent for biological samples. The specific method comprises: adding a sodium hydroxide solution to a biological sample, adding a lysate, and carrying out lysis to obtain a lysis reaction liquid, wherein the lysate contains a guanidine salt, disodium edetate, a surfactant and a buffer agent; and adding an ethanol solution to the lysis reaction liquid, and achieving nucleic acid adsorption, cleaning and elution by using an instrument-free nucleic acid extraction apparatus having a silica gel membrane or a centrifugal column. According to the present invention, the sodium hydroxide is added to pre-treat and then the lysate is added to carry out lysis, such that the protein in the sample can be well dissolved, and the binding of the nucleic acid and the adsorption film is prompted; and the lysate component is optimized, such that the binding capacity of the nucleic acid molecule and the silica gel membrane under the high salt and high pH value conditions is significantly enhanced, and the good nucleic acid extraction efficiency is ensured.

A composite medicine in the form of tablet, capsule, particle, pill, etc for treating bone fracture, injury, contusion, local stasis, soft tissue injury, rheumatic numbness, periomethritis, enosynovitis, gynecological inflammation, menoxenia, etc, and preventing and treating cardiovascular and cerebrovascular diseases is disclosed. Its preparing process is also disclosed.

The invention relates to a red cell storage solution and a preparation method thereof. The storage solution comprises a buffering system, a nutritional system, an osmotic pressure system and an anticorrosion system, wherein the anticorrosion system comprises the following components according to mass concentration: 0.2-1g/L of chloramphenicol, 0.1-0.6g/L of neomycin sulfate, and 0.1-0.5g/L of gentamicin. Through mutual concurrent effects of the buffering system, the nutritional system, the anticorrosion system and the osmotic pressure system of the red cell storage solution provided by the invention, long storage cycle of the red cell storage solution is ensured, at the end of the sixth month of the storage, that red cell suspension free hemoglobin concentration still conforms to national standard is detected, and the hemolysis ratio at the end of the storage is smaller than 0.8 percent of the total of red cells; the red cell storage solution provided by the invention can ensure relatively strong antigenic activity and stability of the red cells.

The invention relates to an erythrocyte preservation fluid. The erythrocyte preservation fluid comprises the following components: 0.2-0.6mg/ml adenine, 20-50mg/ml glucose, 5-15mg/ml sodium citrate, 0.02-0.06mg/ml sodium dihydrogen phosphate, 1.0-3.0mg/ml anticoagulation agents, and 5-20mg/ml film protective agents, wherein the anticoagulation agents are selected from one or more of EDTA-3K and EDDHA salts. By the aid of the erythrocyte preservation fluid, the coagulability of erythrocytes in the preservation process can be reduced, erythrocyte preservation time can be prolonged, and erythrocyte hemolysis can be reduced.

The invention discloses a light-emitting diode (LED) phototherapy oxygenation type haemodialysis machine, which is applied to haemodialysis ultra-filtration for treating diseases of acute renal failure, chronic renal failure, uremia, hypertension, arteriosclerosis, blood hyperviscosity, histanoxia and the like and belongs to the field of medical instruments. The LED phototherapy oxygenation type haemodialysis machine mainly comprises an LED phototherapy oxygenation part device, a blood extracorporeal circulation pipeline, a dialysate supply water pipeline and a circuit controlled by a microcomputer. The machine is characterized in that: the LED phototherapy oxygenation part device is embedded into an arterial cup clamper and a dialyser clamper of the blood extracorporeal circulation pipeline by a plurality of small-sized LED multi-wavelength penetration nails. In the process of haemodialysis, part of or all of lamps in the LED phototherapy oxygenation device can operate according to a specific situation for carrying out on-line irritation on blood in the circulation pipeline by utilizing a principle of strong penetrating power of LED multi-wavelength, so that blood circulation can be improved, agglutination of erythrocytes, platelet aggregation and blood viscosity are reduced, the flow rate of blood is improved, antithrombin is activated, the content of thrombin is reduced, coagulation and hemostasis mechanisms are adjusted, and the aim of treatment is achieved.

The invention relates to a preparation method of powdered grease of macadimia nut oil. The powdered grease is prepared from the following raw materials in parts by weight: 30-32 parts of macadimia nut oil, 58-60 parts of corn glucose syrup, 1.8-2.2 parts of casein sodium salt, 0.8-1.2 parts of monoglyceryl ester, 0.5-0.8 part of sodium stearyl lactate, 1-2 parts of dipotassium phosphate, 0.3-0.6 part of sodium tripolyphosphate, 0.1-0.3 part of sodium hexametaphosphate, 1-3 parts of macadamia nut protein and 2-3 parts of skimmed milk powder. The preparation method comprises the following steps of: blending and stirring solution A (containing oil-phase macadamia nut oil and monoglyceryl ester), solution B (containing corn glucose syrup, sodium stearyl lactate, dipotassium phosphate and skimmed milk powder) and solution C (containing sodium tripolyphosphate, sodium hexametaphosphate, macadamia nut protein and casein sodium salt), and then, homogenizing, spray drying and packaging. The powdered grease disclosed by the invention is high in embedding rate, uniform in particle size, smooth in surface, good in thermal stability and storage stability and long in shelf life.

The invention relates to a low-temperature high-efficiency crude oil pour point depressant, and belongs to the field of petroleum industry. Sepiolite is subjected to secondary roasting after drying, an ion diffusion channel in the sepiolite is widened, in combination with ion exchange and charge effects, the sepiolite is retarded and prevented from settling, the storage stability is improved, acrylic acid and maleic anhydride are used as raw materials, an dispersion effect is improved through steric hindrance and charge effects, and agglomeration and coagulation of heavy components in crude oil are slowed down; and chitosan, stearic acid, sodium bentonite and the like are used as raw materials, larger steric hindrance is generated, stable dispersion performance is provided, phospholipid isadded when a paraffin treating material is prepared, a single phospholipid molecule has more negative charges and can be adsorbed and wrapped on the surface of colloid, microgels have the same charges and repel each other, so that the pour point depression effect is improved, agglutination deposition of components of the crude oil is slowed down, and the storage stability of the crude oil is further improved. The problems of poor viscosity reduction effect and poor storage stability of the conventional pour point depressant at a low temperature are solved.

The invention discloses application of irisin in the preparation of drugs for preventing myocardial ischemia reperfusion injuries. Experimental results show that irisin can decrease the myocardial infarction area caused by ischemia reperfusion, reduce the increase of the contents of lactate dehydrogenase (LDH), troponin (cTnI), creatine kinase (CK), and other myocardial enzyme markers caused by ischemia reperfusion, meanwhile reducing the inflammatory response, myocardial apoptosis, and oxidative stress response caused by myocardial ischemia reperfusion, promote peroxysome proliferator-activated receptor γ nuclear translocation, and inhibit nuclear transcription factor NF-κB nuclear translocation and accordingly decrease myocardial structure injuries and load increase caused by ischemia reperfusion. Therefore, irisin can be used for preventing and decreasing myocardial reperfusion injuries and has important clinical significance on the treatment of myocardial ischemia.

The invention discloses a compound pharmaceutical composition using glucosamine and sodium chondroitin sulfate as main medicaments, and relates to a compound pharmaceutical composition. The compound pharmaceutical composition solves the problems the conventional medicament for treating arthritis has poor treatment effect and large side effect. The compound pharmaceutical composition comprises the glucosamine, the sodium chondroitin sulfate and accessories, wherein the mass ratio of the glucosamine to the sodium chondroitin sulfate is (4-6): 4, the mass ratio of the glucosamine to the accessories is (140-160): 77, and the glucosamine is glucosamine sulfate or glucosamine hydrochloride. The glucosamine and the sodium chondroitin sulfate are synergistic for treating arthropathy, so that inflammation can be effectively resisted, repair of articular cartilages can be accelerated, synthesis of cartilage matrix is promoted, and degradation and destroy of the cartilages are reduced. The composition is applied to the field of arthritis treatment.

In the present invention, there is provided a method for measuring, under an acidic pH condition, a subject substance in a specimen. The method includes: step A for forming a reaction system by mixing the specimen and an antibody against the subject substance in the specimen; and step B for measuring an antigen-antibody reaction in the reaction system, wherein a pH of the reaction system is set to be acidic, and the relationship between a pI of the antibody and the pH of the reaction system is set to be pI>pH. According to the present invention, a measurement accuracy can be enhanced in an immune measurement reaction system basically using an acidic buffer solution, particularly in a region of low concentration for an antigen.

The invention relates to a preparation method of cashew nut oil power grease. The cashew nut oil power grease is prepared from the following raw materials in parts by weight: 28-30 parts of cashew nut oil, 60-62 parts of corn glucose syrup, 1.5-2.5 parts of casein sodium, 0.8-1.2 parts of monoglyceryl ester, 0.3-0.6 part of sodium stearoyl lactylate, 1-2 parts of dipotassium hydrogen phosphate, 0.1-0.5 part of sodium tripolyphosphate, 0.1-0.3 part of calgon, 1-3 parts of cashew nut egg white, and 1-3 parts of skim milk powder. The preparation method comprises the following technology processes: mixing, stirring, homogenizing, spray drying and packing a liquid A composed of an oil phase (the cashew nut oil and the monoglyceryl ester), a liquid B composed of the corn glucose syrup, the skim milk powder, the sodium stearoyl lactylate and the dipotassium hydrogen phosphate and a liquid C composed of the sodium tripolyphosphate, the calgon, the cashew nut egg white and the casein sodium so as to obtain a finished product. According to the preparation method provided by the invention, the embedding rate is high, the surface is smooth, the heat stability and the storage stability are obviously improved, and the shelf life is prolonged.

The invention relates to the field of food and specifically provides a beverage formula. The beverage formula comprises the following components: 34% of dry grains, 10% of whole milk powder, 5% of dextrose, 50% of non-dairy creamer and 1% of food flavor. The beverage formula can quicken blood circulation, increase the compositions of blood volume, stimulate hematopoiesis function of organism, balance water metabolism of intracellular environment, eliminate cell metabolites, maintain normal functions and metabolism of the cell, prompt regeneration of the cell and increase blood circulation, expel pathogenic wind and cold and sweat for detoxification.

The invention discloses an antibody screening reagent for enhancing immunosuppression turbidimetry and a preparation and use method thereof. The method for enhancing immunosuppression turbidimetry overcomes the defects of the method for enhancing immune turbidimetry in the prior art in the principle of immune reaction. When no antigen exists in a measurement reaction system, the antigen connectedto latex particles generates agglutination reaction with the limited amount of antibody in the system, and the agglutination effect is maximized. When an antigen exists in the measurement reaction system, since the amount of antibody is limited, free antigen is excessive antigen which can inhibit the agglutination effect of the antigen-antibody complex, therefore, as the amount of free antigen inthe reaction system increases, the agglutination effect of the antigen-antibody complex is decreased. When the amount of antigen in the measurement system reaches a certain amount, the free antigen will completely inhibit the binding of the antibody and the antigen on the latex, and if the nonspecific binding of excess antigen and other substances in the latex and sample can be overcome, the turbidity change during the reaction will be zero.

The invention discloses a healthful porridge for prevention of gestational diabetes mellitus. The porridge is mainly prepared from the following bulk drugs, by weight: 2-6 parts of guava, 1-5 parts of Malabar spinach, 2-6 parts of propolis, 2-3 parts of garlic essence, 3- parts of pumpkin, 3-6 parts of a Ginkgo biloba extract, 1-3 parts of Astragalus mongholicus and 10-20 parts of millet. The healthful porridge provided by the invention has the advantages of scientific formula, simple preparation method, convenience for usage, and easiness to carry. In addition, the invention has the following excellent effects: 1, the selected materials are all traditional Chinese herbal materials without any toxic or side effect; 2, the prescription is fully compliant with the principles of traditional Chinese medicine and the physical and psychological characteristics patients with gestational diabetes mellitus, and in the premise of the general principle, prescriptions with different functions can be applied to the different groups of people; and 3, the medicinal herbs resources are easily available and low in cost, so as to reduce the burden of patients.

The invention discloses a human urinary albumin latex enhanced secondary-antibody competitive immunity turbidity detection kit as well as a production method and a use method thereof. By utilizing a secondary-antibody competitive immunity turbidity method, the defects of existing enhanced immunity turbidity methods are overcome from immune-reaction principles; when measuring that no antigen existsin a reaction system, a secondary antibody linked to a latex particle reacts with a primary antibody in the system to generate agglutination reaction, and the agglutination effect reaches the maximum; and when measuring that the antigen exists in the reaction system, the antigen is bound with the primary antibody to form an antigen-antibody (primary antibody) immune compound, a binding site between the primary antibody and the secondary antibody is closed by through the binding, the competition is formed with immune agglutination reaction of the primary antibody and the secondary antibody, and if the amount of the primary antibody is limited, the agglutination effect of a primary antibody-secondary antibody immune compound in a sample is competitively inhibited by the antigen, so that theagglutination effect of the immune compound generated through the reaction between the primary antibody and secondary antibody-latex is reduced along the increase of free antibodies in the reaction system.

The invention discloses a preparation method of oral liquid for reducing blood lipid, and belongs to the technical field of processing of health-care foods. The preparation method comprises the following steps of: crushing brown rice to obtain brown rice powder, performing saccharification and filtering on the brown rice powder to obtain brown rice saccharification liquid, and squeezing grapes into grape juice; uniformly mixing the grape juice with the brown rice saccharification liquid to obtain mixed liquid, adding soybean protein peptide to the mixed juice, performing uniform stirring, regulating the pH to 6.8-7.0, performing sterilization under the condition of 90-95 DEG C for 10 minutes, and lowering the temperature to 32-35 DEG C; performing inoculating with monascus purpureus strains from bevels, and performing culturing for 4-5 days so as to obtain monascus purpureus fermentation liquid; refining the monascus purpureus fermentation liquid through a mycelium colloid mill to obtain nutrient slurry; and performing filtering, blending, concentrating, filling, sealing and sterilizing on the nutrient slurry to obtain finished products. The oral liquid not only is good in mouth feel, rich in fruity flavor and safe to drink, but also has notable efficacy of reducing blood lipid.

The invention provides camellia blend oil capable of enhancing immunity and a processing method thereof. The blend oil takes camellia seeds, corns, peanuts,non transgenic soybeans,common sowthistle herb seeds, avocado, almonds and pine nuts as raw materials and prepared through Pretreatment low temperature pressing. Used spiral oil press processing equipment includes a base, an electronic control system, a spiral main shaft, a press cage system, a cooling system and a temperature adjusting system. A press cage is a double-layer hollow structure; a water outlet and a water inlet are arranged on the wall of the double-layer press cage; the water outlet is connected to the top of a water tank, and the water inlet is connected to the bottom of the water tank through a water pump; the water tank includes refrigeration pieces; and a temperature sensor is fixedly installed on the inner side of the water outlet of the double-layer press cage. Thus, the temperature of a press cavity during cold pressing of oil can be detected at any time, the open degree of a control valve can be automatically adjusted according to the detected temperature, so that cold water flow in the internal structure of the double-layer press cage can be controlled, the temperature during oil pressing can be strictly controlled, and the quality of the camellia blend oil can be guaranteed.

The invention relates to a compound amino acid health product, and belongs to the field of health-care food. The compound amino acid health product is prepared from the following raw materials in parts by weight: 2.5-3 parts of L-alanine, 4.5-5 parts of L-glutamic acid, 10 parts of fructose, 15-20 parts of oligoisomaltose, 0.002-0.003 part of vitamin C, 0.0006-0.0008 part of vitamin B1, 0.0002-0.0004 part of vitamin B2, 0.0003-0.0004 part of vitamin B6, 0.0001 part of vitamin B12 and 8-10 parts of alpha-linolenic acid. The invention also provides a preparation method of the compound amino acidhealth product. The compound amino acid health product has a scientific formula and obvious mutual synergistic effects among the components, and has the effects of improving the immunity of human bodies, reducing blood fat, preventing cardiovascular and cerebrovascular diseases, relieving fatigue, enhancing physical strength, protecting eyesight, benefiting intelligence, delaying senescence, dispelling the effects of alcohol and protecting liver, and the like.