The invention discloses essence capable of preventing alopecia and promoting hair growth and a preparation method of the essence capable of preventing alopecia and promoting hair growth. The preparation method comprises the steps of weighing raw materials of dimethyl sulfone and adenosine, and performing uniform dispersion with water; weighing a preservative, and performing heating and dispersion;weighing 1,3-propylene glycol, butanediol, EDTA-2NA and water, performing stirring and heating, and performing natural cooling; weighing a fleeceflower root extract, a ginseng root extract, a cacumenbiotae extract, a rhizoma anemarrhenae root extract, a ginger root extract and a radix angelicae sinensis extract, performing uniform stirring, adding the treated dimethyl sulfone, the treated adenosine and the treated preservative, performing stirring to a uniform state, performing inspection, performing filtration, performing discharging, and performing filling so as to obtain finished products. The preparation method disclosed by the invention is simple in process and low in cost, the residual quantity of effective substances of the finished products is increased, the time of taking effectis shortened, and the effects are strengthened.