74 results about "Biological pump" patented technology

The present invention relates to the field of nucleic acid extraction, particularly to a nucleic acid extraction method and a nucleic acid extraction reagent for biological samples. The specific method comprises: adding a sodium hydroxide solution to a biological sample, adding a lysate, and carrying out lysis to obtain a lysis reaction liquid, wherein the lysate contains a guanidine salt, disodium edetate, a surfactant and a buffer agent; and adding an ethanol solution to the lysis reaction liquid, and achieving nucleic acid adsorption, cleaning and elution by using an instrument-free nucleic acid extraction apparatus having a silica gel membrane or a centrifugal column. According to the present invention, the sodium hydroxide is added to pre-treat and then the lysate is added to carry out lysis, such that the protein in the sample can be well dissolved, and the binding of the nucleic acid and the adsorption film is prompted; and the lysate component is optimized, such that the binding capacity of the nucleic acid molecule and the silica gel membrane under the high salt and high pH value conditions is significantly enhanced, and the good nucleic acid extraction efficiency is ensured.

In a device (1) for rapid pressure-freezing an aqueous sample (3), such as a biological specimen, a pressurized cooling medium can be fed into a high-pressure chamber (11) into which a sample holder (30) containing a sample (3) is inserted and which is sealed with a pressure-tight seal, to the location of the sample holder (30) held therein. The high-pressure chamber (11) comprises a viewing window structure (2) with a pressure-tight window (20), through which light can be directed from the outside onto the sample (3) located in the sample holder (30). The window (20) can comprise a transparent window element made of a high-pressure-resistant material, wherein the window element (20) is held by a pressure- and temperature-resistant window bearing provided in the high-pressure chamber.

The present invention discloses a column for incubating and/or isolating chemical and/or biological samples. Furthermore, a method for incubating and/or isolating chemical and/or biological samples as well as a method for separating liquids and solids from a disperse mixture is subject-matter of the present invention.

The present invention relates to a kind of magnetic separating column and its application in separating biological samples. The magnetic separating column has structure comprising a material holding section, a filler column, a discharge port and a pushing plug. The filler column is filled with small magnetic soft iron balls of sizes 0.01-0.1mm, 0.1-0.2mm and 0.2-1mm separately. During separation, the sample fed through the inlet in the upper end is made to flow through the filler column and the discharge port under the gravity action, and the pushing plug applies pressure to push out the blocked sample. Under the action of outer magnetic field, the magnetic separating column can generate high gradient magnetic field for effective fast separation of various kinds of cells, subcellular matters, bacteria, viruses, nucleic acids, proteins and other biological samples.

The invention relates to a method for efficiently separating and purifying multiresidue sulfonamide antibiotics in a biological sample. The method is characterized by comprising the following steps of: (1) preparing a molecular imprinted polymer, comprising the steps of burdening according to the mol ratio of template molecule to functional monomer to cross linking agent of being 1: (2-8): 25 and obtaining the molecular imprinted polymer with special identification function to the sulfonamide antibiotics by adopting a body polymerization method; (2) weighing certain amount of biological sample to be separated into a mortar, grinding the biological sample by the mortar, adding right amount of the molecular imprinted polymer, and further homogenating to obtain a homogenated sample, wherein the weight ratio of the biological sample to be separated to the molecular imprinted polymer is 2: (1-5), and (3) placing the homogenated sample in a solid phase extraction column, sequentially rinsing with 2.0-4.0ml of water, 2.0-4.0ml of 10wt% acetonitrile aqueous solution, and then eluting with 3.0-5.0ml of methanol/methanoic acid mixed solution; and separating the sulfonamide antibiotics from the biological sample to be separated by being instantly dissolved in the methanol/methanoic acid mixed solution. The method provided by the invention has wide selectivity and good separation and purification effect.

The invention belongs to enrichment organism post treatment technology in the technical field of resource and environment to adopt mushroom fungus-plant for jointly renovating soil polluted by heavy metal. In the post treatment technology of the invention, plant corpus and mushroom fungus fruiting body enriched in heavy metal are collected; the plant corpus and mushroom fungus fruiting body are roughly ground according to a certain proportion to be used as fermentation material of methane and thrown into a methane tank for decrement by fermentation; the heavy metal in the methane liquid is removed by chemical precipitation, methane residue and heavy metal precipitation with concentrated heavy metal are intensively buried. In the invention, the methane tank is used as an organism decrement device, the characteristics of low cost, mature technology, easy promotion and high decrement rate of methane fermentation are adopted to carry out post treatment of biological concentration heavy metal without secondary pollution; the general effect is that enrichment organism decrement rate is 70-90% and removal rate of heavy metal in the enrichment organism is over 90%.

The invention relates to an isotope dilution high resolution chromaticness combination method for simultaneously detecting organic chlorine pesticides and polychlorinated biphenyl in a biological sample. The method can realize simultaneous purification of the two targets of the organic chlorine pesticides and the polychlorinated biphenyl in a pre-treatment process, and a detection process can be carried out simultaneously. The sample pretreatment employs a method combining gel permeation chromatography with Florisil silica column (or silica gel column) purification. A gold standard method using an isotope dilution high resolution GC-MS is employed for the detection. Compared with a traditional low resolution mass spectrum and an electron capture detector (ECD) method, the interference and false positive results are reduced, and the indexes such as the method deletion limit and the quantitative result accuracy are greatly improved. The method can save more than 50% of labour amount, time and consumables such as solvents, and filler materials in comparison with a method for respective and step by step measurement of the organic chlorine and the polychlorinated biphenyl, and has important meaning for research and monitor of the organic chlorine pesticides and the polychlorinated biphenyl.

The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and/or hydrophobic interaction.

The invention discloses a method for purifying RNA (ribonucleic acid) in a biological sample by a selective filtration column. The method specifically comprises the following steps of centrifuging anddelaminating the biological sample treated by an RNA lysis buffer at high speed, adding an upper water phase into an inner tube of the selective filtration column, and centrifuging the selective filtration column in a centrifuging machine; adding 5 to 100 microliters of water into the inner tube of the selective filtration column, oscillating for 30s, and transferring a water solution in the inner tube of the selective filtration column into a centrifuge tube without RNA enzyme, wherein the solution in the centrifuge tube is the purified RNA solution; enabling the RNA lysis buffer to lyse thecells of the biological sample, centrifuging and delaminating at high speed, removing cell fragments and DNA (deoxyribonucleic acid), enabling the RNA to completely dissociate in the upper water phase, and enabling the RNA to pass through a bottom ultrafiltration membrane of the selective filtration column, wherein the ultrafiltration membrane only has permeability on micromolecular substances and no permeability on RNA of biological macromolecular substance under the high-speed centrifuging condition, so as to realize the purifying of the RNA of the biological macromolecular substance.

An appliance for rapidly vibrating test tubes containing samples to be ground up, the appliance comprising an electric motor (12) for driving rotation of a disk (16) that is provided with an eccentric pin (17), the appliance being characterized in that the test tubes (23) are perpendicular to the eccentric pin and are held by a clamp (21) mounted on a support (20) that is substantially parallel to the eccentric pin, being connected to a fixed baseplate via a Cardan type hinge (15) having two mutually perpendicular axes of rotation (X and Y), one of which axes (Y) is substantially parallel to the eccentric pin (17) and connects the support (20) to the other axis (X) of the hinge (15) that prevents the support (20) from moving in rotation about a perpendicular axis (Z). The support (20) is connected to the eccentric pin (17) by a link (19).

The invention relates to a device for purifying biological samples and application thereof. The device comprises a strong cationic or anionic switching trap column, a connecting component and a reverse trap column and can realize simultaneous removal of strong cationic or anionic surfactant or ionic liquid and salt in biological samples. The device has the advantages of high efficiency, high recovery rate, fastness and simple operation, and can be widely used in the purification of protein samples.

The invention relates to an embedded biological pre-oxidization aerated filter advanced-treatment method, which is characterized in that an ozone aeration upgrading technology is combined with a BAF (Biological Aerated Filter), and feed water and refluxed fluid from an oxidization area enter a biochemical treatment area of the BAF through a water distribution area to finish the biological degradation of pollutants such as biochemically-treatable organic matters, ammonia nitrogen and nitrate nitrogen; a part of supernatant obtained by biochemical treatment is drained from a device as effluent; the other supernatant automatically flows into a refluxing area through a flow guide tube, and enters the oxidization area after being upgraded by ozonized air in an upgrading area, and the effluent in the oxidization area and the feed water enter the water distribution area of the BAF, and enter the biochemical treatment area of the BAF for biochemical reaction after being mixed, wherein the oxidization area is filled with an active catalyst.

The invention provides a pretreatment method for pesticide residue detection in a biological sample, and relates to the field of pesticide residue analysis in environmental organism. The method comprises the following steps: mixing a homogenate of a biological sample to be detected with water to obtain a first mixture; mixing the first mixture directly with acetonitrile for adsorption, and obtaining a second mixture comprising an acetonitrile layer and a water layer; mixing the second mixture directly with a purified adsorption material, and reacting for more than 1 min; and aggregating the purified adsorption material by external magnetic field adsorption, separating a supernatant for filtration, and the obtained filtrate is a to-be-detected liquid for pesticide residue detection. The purified adsorption material comprises Fe3O4-TiO2, GCB, MgSO4 and NaCl. According to the pretreatment method for the pesticide residue detection in the biological sample, a suitable water addition ratiofor the biological sample having high protein content and complex impurity components is provided. In the process of the pretreatment method of the invention, the purification and extraction steps arecombined to shorten the time and improve the detection precision, and high-throughput detection can be realized.

The invention discloses a treatment method of primary gold ore. The method includes: biologically pre-oxidizing sulfide minerals of the primary gold core, drip-leaching leaching columns with dilute sulphuric acid solution at the speed of 10 L/(m<2>.h) to 50 L/(m<2>.h), and after pH of outgoing liquid is equal to that of dilute acid, adding cultured germs to allow biological pre-oxidization; after biological pre-oxidization, washing the columns with clear water, and performing a cyanidation leaching experiment. The pH value of cyanidation leachate is controlled to 10 through 11, and leaching speed is controlled to 15 L/(m<2>.h) through 25 L/(m<2>.h). Leaching efficiency of the primary gold core subjected to biological oxidation treatment is high, and the treatment is environment friendly; a new way, effective, feasible and environment friendly, is provided for the heap leaching production of gold-bearing sulfide ores.

Systems and methods of sample (1) and staining processing including compression and dynamic movement of liquids (3) in a fluidically moving substantially contained liquid bridge (6) perhaps between a hydrophobic wand (4) and a hydrophilic sample support element (2). Embodiments may include low volume reagent and perhaps even low volume buffer wash in sample processing. In addition, antibodies can be conjugated with nanoparticles (64) and can be used in sample processing. Exposing a sample with or without movement to AC, DC, or even a permanent magnet field may improve staining. Staining with nanoparticle reagents could be quantified using a microscope with a magnetometer below the slide viewing area. The detection of nanoparticles attached to the chemistry may facilitate the quantification of cancerous cells stained in the tissue.

The invention discloses a method and system for combined remediation of polycyclic aromatic hydrocarbon-polluted soil in an in-situ chemical and biological mode. The method includes the following steps that (1) carbon-coated porous foam iron subparticles and a mixture of sodium perborate or urea peroxide and citrate are scattered into the polluted soil; (2) a hydrogen peroxide solution is sprayedinto the polluted soil, and after pollutants in the soil are fully degraded, the carbon-coated porous foam iron subparticles are recycled; and (3) degrading bacterium suspension is sprayed into the polluted soil. The system comprises a material scattering device and a recycling device. The material scattering device comprises a material scattering movable frame. The material scattering movable frame is provided with a mixture hopper, a hydrogen peroxide solution storage tank and spray heads. The recycling device comprises a recycling movable frame. The recycling movable frame is provided witha magnetic recycling device, a recycling tank, a mixed bacterium suspension storage tank and spray heads. By adoption of the method and system, the process is simple, cost is low, sustainability is high, damage to soil microorganisms and organic substances is evaded and the danger of secondary pollution can be avoided.

A method for isolating a biological target material from a biological sample is provided, the method including binding the target material to a solid support, wherein impurities are selectively removed from the solid support by washing the latter with a wash buffer containing a cationic amine. A kit for isolating a biological target material from a biological sample is also provided, the kit including a wash buffer containing a cationic amine, and the use of a respective wash buffer.

The invention discloses a full-sea-depth macro-organism pumping type fidelity collecting and storing system which comprises a pressure maintaining assembly, a sampling assembly, a circuit barrel, a culture kettle and a collecting barrel. The sampling assembly is inserted into the pressure maintaining assembly, and the sampling assembly is connected with the culture kettle. An inlet sealing assembly is arranged at an inlet in the middle of the pressure maintaining assembly, and the collecting barrel is connected with the pressure maintaining assembly through the inlet sealing assembly. A heat preservation assembly is bonded to the outer wall of the pressure maintaining assembly, a water pump and a pressure compensation device are arranged on the outer wall of the bottom of the pressure maintaining assembly, the pressure compensation device is communicated with the pressure maintaining assembly, and the water pump is communicated with the pressure maintaining assembly through a guide pipe. A temperature sensor and a pressure sensor are arranged on the inner wall of the pressure maintaining assembly. The circuit barrel is installed on the outer wall of the pressure maintaining assembly, a power source and a controller are arranged in the circuit barrel, and the controller is electrically connected with the temperature sensor, the pressure sensor, the heat preservation assembly andthe water pump. According to the invention, full-sea-depth macro-organism trapping and heat and pressure preservation culture can be realized, and the in-situ vital signs of the full-sea-depth macro-organisms can be effectively ensured.

The invention discloses a method for detecting genistein and metabolites thereof in a biological sample, which is characterized by comprising the following steps: (1) processing the biological sample;and (2) detecting a to-be-detected solution by an ultra high performance liquid chromatography-mass spectrometer. The detection method disclosed by the invention can detect more genistein metabolitesin a biological sample, thereby laying a foundation for clarifying the metabolic pathways of genistein.

The invention relates to a sleeve for biological sample in-vitro purification treatment. The sleeve comprises a pipe body, multiple movable baffles, a connecting shaft, a sealing ring and a coaxial locking switch, wherein the pipe body is cylindrical, a flange is arranged at the upper end of the pipe body, multiple first openings are formed in the end face of the bottom of the pipe body, and multiple second openings and convex blocks are arranged at the bottom end of the side wall of the pipe body; the sealing ring is sleeved on the outer side of the second openings; the movable baffles are arranged at the bottom of the pipe body, are of an annular fan shape and are provided with multiple third openings, and the outer edges of the third openings are in one-to-one correspondence with the second openings and are contacted with the sealing ring; the connecting shaft is positioned at a central position of the pipe body, the top end of the connecting shaft is connected with the coaxial locking switch, a polygonal part is arranged at the bottom end of the connecting shaft, and the edges of the polygonal part are in one-to-one correspondence with the inner edges of the movable baffles; the coaxial locking switch is positioned on the upper surface of the flange of the pipe body and can rotate. The sleeve provided in the invention is a device which is convenient, practical, low in production cost, simple in production process, safe and non-toxic and is used for biological sample in-vitro treatment.

The invention relates to a degradable foamed co-extruded PET barrier film. The degradable foamed co-extruded PET barrier film is characterized in that materials of all layers in the laminated film have approximately-consistent biodegradability through introducing biological groups, and the added amount of an additive master batch in the materials of each layer is controlled to be 0.3-15% the total mass of the materials of the corresponding layer; the hydrophilic activity of hydrophilic groups in the additive master batch needs to be greater than or equal to that of the hydrophilic groups in the materials of each layer; through adding the additive master batch, the mole ratio of the hydrophilic groups and carbon atoms of the materials of each layer is approximately consistent, namely that the biological activity is approximately consistent, so that the degradation rate of the materials of all the layers in the laminated film is approximately consistent. The degradable foamed co-extruded PET barrier film has the contributions that through balancing the mole ratio of the hydrophilic groups and carbon atoms of the materials of each layer, the approximately-consistent biological activity and approximately-consistent biodegradation rate are obtained, and the appearance, functions and physical and mechanical properties of products are kept invariable.