Device for purifying biological sample and application thereof

A technology for biological samples and samples, applied in the preparation of test samples, etc., can solve the problem of inability to remove two types of substances at the same time, and achieve the effect of high recovery rate

Active Publication Date: 2014-12-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently used chromatographic method can only remove surfactants or salts...

Method used

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  • Device for purifying biological sample and application thereof
  • Device for purifying biological sample and application thereof
  • Device for purifying biological sample and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] 1. Device construction: The strong anion exchange packing (TSK-GELSuper Q-5PW, 10μm, 1000 ) And C18 reverse phase filler (Beijing Aijieer, Venusil XBP-C18, 5μm, 120 ) Respectively fill the two trapping columns 3 and 5, and press each component figure 1 connection.

[0035] 2. The balance of the trapping column: Press figure 1 As shown (above), use syringe 1 to inject 50 times the column volume of 0.1% (volume fraction) formic acid aqueous solution through injection head 2.

[0036] 3. Purification and enrichment of samples: press figure 1 As shown (above), use syringe 1 to inject 500μL of bovine serum albumin (BSA) digestion product (containing 0.5% SDS, 20mM NH) with a molar concentration of 0.1mg / mL through the injection head 2. 4 HCO 3 ), and then use the syringe 1 to inject 5 times the column volume of 0.1% (volume fraction) formic acid aqueous solution, the BSA enzymatic hydrolysis products are trapped and enriched on the reversed-phase trap column 5.

[0037] 4. Sam...

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Abstract

The invention relates to a device for purifying biological samples and application thereof. The device comprises a strong cationic or anionic switching trap column, a connecting component and a reverse trap column and can realize simultaneous removal of strong cationic or anionic surfactant or ionic liquid and salt in biological samples. The device has the advantages of high efficiency, high recovery rate, fastness and simple operation, and can be widely used in the purification of protein samples.

Description

Technical field [0001] The invention relates to a biological sample purification device and its application, which can realize the simultaneous removal of strong cationic or strong anionic surfactants or ionic liquids and salts in biological samples. The device has the advantages of high efficiency, high recovery rate, fast speed and simple operation, and can be widely used in the purification of protein samples. Background technique [0002] Surfactants are a class of substances that can significantly change the surface tension of liquids or the interfacial tension between two phases. Their molecules contain two components, hydrophilic and hydrophobic. Surfactants are generally classified into cationic surfactants, anionic surfactants and nonionic surfactants. Because surfactants have a strong solubilizing ability, they are often used as solubilizers for biological samples to dissolve insoluble biological samples such as membrane proteins (Wisniewski, JR; Zougman, A.; Nagaraj, ...

Claims

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Application Information

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IPC IPC(8): G01N1/34
Inventor 张丽华赵群杨开广吴慈张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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