19 results about "Strong anion exchange" patented technology

The invention belongs to the technical field of anion exchange membranes, and relates to a cross-linked polyaromatic piperidine anion exchange membrane and a preparation method thereof. The exchange membrane structure comprises a copolymer structure of the following repetitive structural units, the selected rigid main chain polyaromatic hydrocarbon can ensure the mechanical strength of the anion exchange membrane, meanwhile, the toughness of the anion exchange membrane is further enhanced by adopting a cross-linked structure, and a formed cross-linked network can reduce swelling of the anion exchange membrane, so that the anion exchange membrane is more stable in performance. The mechanical performance is improved; according to the invention, ether-free skeleton polyaromatic hydrocarbon is selected as a main chain and has the characteristic of strong alkali-resistant stability, six-membered nitrogen heterocyclic ions are adopted as functional groups, and a large steric hindrance structure can hinder nucleophilic substitution and elimination reaction, so that the alkali-resistant stability of the anion exchange membrane is improved, and the service life of the anion exchange membrane is prolonged. And in the # imgabs0 #, X is equal to 0.05 to 0.15.

A method for separating protein from a milk raw material, the method comprising separating beta-lactoglobulin and alpha-lactalbumin from the milk raw material, the method comprising: a whey separation step for separating whey protein from the milk raw material; a step of ion exchange treatment: adsorbing the whey protein by using strong anion exchange resin, then eluting by using an eluent, adsorbing the whey protein by using strong anion exchange resin to obtain a flow-through liquid, enriching alpha-lactalbumin in the flow-through liquid, and carrying out gradient elution by using the eluent to obtain an elution component, at least one of the elution components is enriched with beta-lactoglobulin; and purifying the flow-through liquid to obtain an alpha-lactalbumin solid, and purifying the elution component enriched with beta-lactoglobulin to obtain a beta-lactoglobulin solid.

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

The application discloses a preparation and purification method of high-purity radioiodine-labeled antigen. The method comprises the following steps: pre-activating carrier-free sodium iodine[125I] solution in an orthogonal solvation sensitization buffer containing lithium sulfate, 18-crown-6 and beta-cyclodextrin; adding antigen and chloramine-T for a labeling reaction; and performing magnetic separation and purification by using a suspension containing adamantane-modified magnetic microspheres and quaternary ammonium salt-modified strong anion exchange magnetic microspheres after the reaction. The orthogonal solvation effect is used to enhance the nucleophilic activity of iodine ions, and the amount of oxidizing agent is reduced to reduce protein damage; impurities are quickly removed through the double mechanisms of electrostatic adsorption and host-guest recognition. The application has the advantages of high labeling efficiency, high radiochemical purity of the product and complete retention of biological activity.

The application discloses a porous substrate reinforced anion exchange membrane and a preparation method and application thereof, relates to the technical field of anion exchange membranes, and discloses a porous substrate reinforced anion exchange membrane which is obtained by uniformly mixing a polyarylene alkylidene cationic polymer solution and a C1-C10 alcohol solvent, coating the mixture on both sides of a porous substrate, and removing the solvent. The application does not need to pretreat the porous substrate, can realize good infiltration of the cationic polymer solution to the porous substrate, and further obtains a porous substrate reinforced anion exchange membrane with a sandwich structure. The thin film has outstanding anti-swelling property and excellent mechanical property, and has good electrochemical performance, and has a good application prospect in an alkaline membrane energy conversion device.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

The invention discloses a method for producing N-acetylgalactosamine sulfate by degrading chondroitin sulfate through two-step enzyme catalysis, and belongs to the technical field of biology. The preparation method comprises the following steps: firstly, splitting chondroitin sulfate by using lyase to generate unsaturated chondroitin sulfate disaccharide, and then reacting the obtained unsaturated chondroitin sulfate disaccharide with disaccharide hydrolase; enabling an unsaturated bond to break and hydrolyze to generate two monosaccharides, namely N-acetyl galactosamine sulfate (monosaccharide) and 4-deoxy-L-threo-5-hexanone uronic acid; and finally, separating the N-acetyl galactosamine sulfate (monosaccharide) from the 4-deoxy-L-thre-5-hexuronic acid by virtue of strong anion exchange column chromatography, collecting an elution peak, and carrying out freeze-drying, so as to obtain the high-purity N-acetyl galactosamine sulfate. The method provided by the invention is green and environment-friendly, has strong specificity and high conversion rate, is suitable for industrial application, can be widely applied to the fields of medicines and health care products, and has important market value.

A new type of test for DNA damage, referred to as Enhanced Specificity Mass Tag DNA Adductomics (「ESMD」), which results in advanced DNA adductomics, defines genotoxic exposure accurately and extensively in a practical way, including specific combinations of mass tags targeting amines and techniques selected from strong anion exchange, gentle depurination, and affinity chromatography.

The invention discloses long alkyl chain strong anion exchange resin and a preparation method thereof, which are characterized in that a double bond-containing polymerizable monomer, a cross-linking agent and a halogen-containing polymerizable functional monomer are used for preparing a halogen functionalized microsphere matrix by using a suspension polymerization or seed swelling method, and then the halogen functionalized microsphere matrix reacts with a long alkyl chain amino functional monomer to obtain the long alkyl chain strong anion exchange resin. And finally, carrying out post-modification by using a halogen-containing long alkyl chain quaternary ammonium salt to obtain the novel long alkyl chain strong anion exchange resin. The long alkyl chain strong anion exchange resin synthesized by the method has the advantages of simple synthesis process, controllable number of quaternary ammonium groups, no use of toxic organic reagents, and large-scale preparation.

This invention discloses a method for extracting target products from dairy raw materials, relating to the field of food processing technology. Before strong anion exchange chromatography, this method introduces a synergistic pretreatment step of ultrafiltration and conductivity adjustment, achieving "speed increase, efficiency enhancement, quality improvement, and cost reduction" in the chromatography process. It overcomes the technical bottlenecks of traditional ion exchange chromatography in processing dairy raw materials, such as slow speed, susceptibility to contamination, and instability, providing a solution for the efficient, high-purity, and large-scale production of β-lactoglobulin and α-lactalbumin. This method can also combine strong and weak anion exchange chromatography to further improve the extraction purity of β-lactoglobulin and α-lactalbumin. Furthermore, this method can simultaneously achieve efficient extraction of multiple target substances such as casein micelles, lactose, and milk mineral salts, thereby significantly increasing the added value and resource utilization efficiency of dairy by-products.

This invention relates to the field of fuel cell technology and discloses a glass fiber cloth-reinforced anion exchange membrane and its preparation method, comprising the following steps: S1, brominated polyphenylene ether is subjected to quaternization treatment to obtain a quaternization-modified brominated polyphenylene ether film-forming solution; S2, glass fiber cloth is immersed in the film-forming solution and cast to form an anion exchange membrane; in S1, the synthesis process of brominated polyphenylene ether is that alkylphenol is brominated and polymerized to obtain brominated polyphenylene ether. This invention synthesizes a brominated polyphenylene ether with a high degree of bromine substitution, and then subjectes it to quaternization treatment, resulting in a higher degree of quaternization and a greater number of cationic groups, thus achieving a higher ion exchange rate; at the same time, glass fiber cloth is used to improve the mechanical properties of the anion exchange membrane.

Certain combinations of amine-targeting mass tags with techniques selected from strong anion exchange, mild depurination, and affinity chromatography give advanced dna adductomics.

This application relates to the field of chromatography technology, specifically to anion exchange chromatography membrane, its preparation method, and its application. The membrane includes a porous support and anion exchange groups attached thereto. A first surface and a second surface are formed on both sides of the porous support. The anion exchange groups include strong anion exchange groups and weak anion exchange groups. The strong anion exchange groups are quaternary ammonium groups, and the weak anion exchange groups are one or more of primary, secondary, and tertiary amine groups. The nitrogen content of the first surface of the anion exchange chromatography membrane is in the ratio of nitrogen from the strong anion exchange groups to nitrogen from the weak anion exchange groups at (1-5):1. This invention, by controlling the inclusion of both strong and weak anion exchange groups and limiting their ratio, results in anion exchange chromatography membranes with high cycle life and good purification effects when used for monoclonal antibody purification.

The invention provides a nanofiber aerogel material for strong anion exchange and a preparation method of the nanofiber aerogel material. The preparation method comprises the following steps: firstly, co-crosslinking PVA-co-PE nanofibers and polyethyleneimine to obtain nanofiber aerogel, then immersing the aerogel material in a non-polar solvent solution of trimesoyl chloride for interface reaction, then drying, then immersing in a hydroxyl quaternary ammonium salt aqueous solution for grafting quaternary ammonium groups, and finally drying to obtain the PVA-co-PE nanofiber aerogel. And finally, obtaining the nanofiber aerogel material for strong anion exchange. Amino functional groups of PEI and acyl chloride groups of TMC are subjected to interface reaction, and then quaternary ammonium groups are grafted on the surface. The PEI / PVA-co-PE composite aerogel has abundant pore channels, and meanwhile, PEI has abundant primary amine and tertiary amine groups and is grafted with a large number of quaternary ammonium groups, so that the ionic strength of the aerogel material is greatly improved, the anion exchange performance is improved, and a selective adsorption function is realized. The nanofiber aerogel material is high in adsorption efficiency, large in adsorption capacity and good in repeatability.

The application belongs to the technical field of exosome purification and characterization, and particularly relates to an exosome purification method and a quality control method suitable for industrial level. The application establishes an exosome purification process combining TFF and whole strong anion exchange chromatography, and selects a nuclease to remove chromatin contamination, aiming to provide an alternative reference for industrial level large-scale preparation of natural exosomes or engineered exosomes for clinical purification. Meanwhile, the application also establishes a whole exosome quality control system combining NTA, WB, TEM, ExoView and FFF-MALS-DLS. By using the exosome purification method and the quality control method, the physical properties and biological attributes of exosomes in the sample can be linked, the most accurate exosome characterization under the current technological conditions can be realized, the quality control of exosome samples at each stage of the process can be realized, and exosomes with almost no chromatin contamination can be obtained.

The present invention discloses a two-dimensional liquid chromatography-mass spectrometry (LC-MS) method for the quality analysis of low molecular weight heparin, which comprises the following steps: 1) Using low molecular weight heparin as a raw material, various low molecular weight heparin oligosaccharides with different degrees of polymerization are prepared by medium pressure column chromatography fractionation; 2) Qualitative analysis of low molecular weight heparin oligosaccharides is carried out by two-dimensional liquid chromatography online connected to mass spectrometry, that is, strong anion exchange chromatography is used in the first-dimensional liquid chromatography to separate low molecular weight heparin oligosaccharides with a single degree of polymerization, each SAX-separated oligosaccharide peak is cut into the second-dimensional molecular sieve chromatography, and finally, online mass spectrometry analysis is carried out; 3) Comparing the SAX standard spectra of different samples with the same degree of polymerization, and semi-quantitatively analyzing each oligosaccharide with a certain degree of polymerization through the peak area. This method can perform rapid and precise structural analysis and quality control of low molecular weight heparin, and has great application potential.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

The present application belongs to the field of bioengineering technology, and particularly relates to a culture method of PDRN genetically engineered cells, a product purification method and application. The present application solves the problems of low yield, growth-synthesis imbalance and poor purification effect in the production of existing recombinant PDRN. During the culture, the fermentation medium is optimized, the nucleotide metabolic pathway is up-regulated in cooperation with a low-dose PDRN synthesis precursor and a metabolic activator, and the pH and dissolved oxygen of the fermentation are controlled in stages to maintain the balance between the growth of the bacterial cells and the synthesis of the product. During the purification, the fermentation broth is pretreated, then subjected to microfiltration, ultrafiltration, Q-agarose gel FF strong anion exchange chromatography and freeze-drying. The present application improves the yield of PDRN, and the purity and yield of the finished product are high, which is suitable for industrial production. The obtained PDRN can be used in the fields of biomaterials, medicine, medical aesthetics and cosmetics.

The invention discloses a strong anion chromatography medium as well as a preparation method and application thereof. The strong anion chromatography medium comprises a matrix and a mixed functional ligand bonded on the surface of the matrix through a carbon-carbon double bond active group, the mixed functional ligand is formed by copolymerizing a charged monomer and a neutral monomer, the charged monomer contains a strong anion exchange group, and the neutral monomer is uncharged. Mixed functional groups are constructed on the microscopic surface of the strong anion chromatography medium, in the purification process of a nucleic acid sample, the strong anion chromatography medium has a strong anion effect and also has a certain hydrophobic effect and hydrogen bond acting force, and under the comprehensive acting force, the nucleic acid sample is separated from impurities, and the resolution ratio is high. The chromatography medium has excellent comprehensive performance, and is especially suitable for separation and purification of nucleic acid samples with more impurities and lower purity. The preparation method is simple, the raw material source is wide, and the application prospect is wide.