115 results about "Strong anion exchange" patented technology

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.

The invention discloses a large-scale preparation method for foot-and-mouth disease totivirus marked vaccine with high yield, high purity and high safety and a product thereof. The method comprises the following steps: a)collecting a virus solution; b)performing deep filtration on a membrane, performing ultrafiltration and performing enzymolysis on nuclease; c)purifying through a strong anion exchange adsorption bed or an adsorption film; d)depositing by PEG, extracting by chloroform-isoamyl aleohl; e)inactivating; F)performing density gradient centrifugation on an inactivation liquid through cane sugar and purifying; g)performing ultrafiltration dialysis and aseptic filtration; and h)reserving a stock solution or emulsifying. The provided foot-and-mouth disease totivirus marked vaccine antigen is uniform and complete foot-and-mouth virus particle, The vaccine is injected into body, so animal infection and immunization can be completely distinguished, does not contain foot-and-mouth disease virus non-structural protein and other virus particle, and does not contain animal-based foreign protein, polypeptide and oligopeptides, animal latent anaphylactic reaction, carcinogenesis and latent risk such as mad cow disease for causing animal infectious diseases due to vaccine injection can be effectively reduced, and the vaccine has no influence on animal food safety and trade.

The invention discloses tea polyphenol oxidase isozyme monomers PP01 and PP02 and a preparation method thereof. With respect to the technical states that existing tea polyphenol oxidase is low in purification factor, low in loading quantity of protein samples and long in operation time, the obtained isozyme is the isozyme with one relative molecular weight, and the isozyme monomer with specific amino acid sequence structure, molecular weight, isoelectric point, enzymatic property and the like cannot be obtained, two tea polyphenol oxidase isozyme monomers with specific specific amino acid sequence structures, molecular weight, isoelectric points, enzymatic properties and the like are separated and prepared by sequentially adopting the technologies and optimization processes such as enrichment of zymoprotein with acetone powder, fractional precipitation of ammonium sulfate, strong anion exchange chromatography, gel medium chromatography, molecular identification and enzymatic property analysis. The method has the advantages of low cost, large preparation amount, high pertinence, short operation cycle and the like, and is of important theoretical and practical significance on deepening of the separation and purification technology of tea polyphenol oxidase isozyme, promoting of directional enzymatic synthesis of a theaflavin component by virtue of the tea polyphenol oxidase isozyme monomers, development and utilization of advantaged isozyme of tea polyphenol oxidase, isozyme immobilization, enzyme protein structure analysis, enzyme gene cloning, functional verification and the like.

The invention provides a separation and purification method for isomers of an anthocyanin compound in lycium ruthenicum murr. Particularly, the method is to efficiently separate and purify the isomers of the anthocyanin compound from a lycium ruthenicum murr extract by adopting mixed-mode chromatography; a mixed-mode chromatographic column is an inverted-phase / strong anionic exchange mixed-mode chromatographic column; efficient separation of the anthocyanin isomers is realized under a low-pH condition by adopting the mixed-mode chromatography; a linear gradient, stepwise gradient or isocratic elution mode is used. A lycium ruthenicum murr ethyl alcohol extract is selected for inversed phase chromatographic preparation and separation to obtain 2 fractions; two pairs of cis-trans anthocyanin isomers, including a new anthocyanin and 3 anthocyanin monomer compounds separated from the lycium ruthenicum murr at the first time, are prepared through the mixed-mode chromatography. The method is excellent in separation effect on the cis-trans anthocyanin isomers, and can realize efficient separation and purification of the anthocyanin isomers and provide a substance basis for research on the activity of an anthocyanin compound.