9 results about "Strong anion exchange" patented technology

The application discloses a preparation and purification method of high-purity radioiodine-labeled antigen. The method comprises the following steps: pre-activating carrier-free sodium iodine[125I] solution in an orthogonal solvation sensitization buffer containing lithium sulfate, 18-crown-6 and beta-cyclodextrin; adding antigen and chloramine-T for a labeling reaction; and performing magnetic separation and purification by using a suspension containing adamantane-modified magnetic microspheres and quaternary ammonium salt-modified strong anion exchange magnetic microspheres after the reaction. The orthogonal solvation effect is used to enhance the nucleophilic activity of iodine ions, and the amount of oxidizing agent is reduced to reduce protein damage; impurities are quickly removed through the double mechanisms of electrostatic adsorption and host-guest recognition. The application has the advantages of high labeling efficiency, high radiochemical purity of the product and complete retention of biological activity.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

The invention discloses a method for producing N-acetylgalactosamine sulfate by degrading chondroitin sulfate through two-step enzyme catalysis, and belongs to the technical field of biology. The preparation method comprises the following steps: firstly, splitting chondroitin sulfate by using lyase to generate unsaturated chondroitin sulfate disaccharide, and then reacting the obtained unsaturated chondroitin sulfate disaccharide with disaccharide hydrolase; enabling an unsaturated bond to break and hydrolyze to generate two monosaccharides, namely N-acetyl galactosamine sulfate (monosaccharide) and 4-deoxy-L-threo-5-hexanone uronic acid; and finally, separating the N-acetyl galactosamine sulfate (monosaccharide) from the 4-deoxy-L-thre-5-hexuronic acid by virtue of strong anion exchange column chromatography, collecting an elution peak, and carrying out freeze-drying, so as to obtain the high-purity N-acetyl galactosamine sulfate. The method provided by the invention is green and environment-friendly, has strong specificity and high conversion rate, is suitable for industrial application, can be widely applied to the fields of medicines and health care products, and has important market value.

This invention discloses a method for extracting target products from dairy raw materials, relating to the field of food processing technology. Before strong anion exchange chromatography, this method introduces a synergistic pretreatment step of ultrafiltration and conductivity adjustment, achieving "speed increase, efficiency enhancement, quality improvement, and cost reduction" in the chromatography process. It overcomes the technical bottlenecks of traditional ion exchange chromatography in processing dairy raw materials, such as slow speed, susceptibility to contamination, and instability, providing a solution for the efficient, high-purity, and large-scale production of β-lactoglobulin and α-lactalbumin. This method can also combine strong and weak anion exchange chromatography to further improve the extraction purity of β-lactoglobulin and α-lactalbumin. Furthermore, this method can simultaneously achieve efficient extraction of multiple target substances such as casein micelles, lactose, and milk mineral salts, thereby significantly increasing the added value and resource utilization efficiency of dairy by-products.

Certain combinations of amine-targeting mass tags with techniques selected from strong anion exchange, mild depurination, and affinity chromatography give advanced dna adductomics.

This application relates to the field of chromatography technology, specifically to anion exchange chromatography membrane, its preparation method, and its application. The membrane includes a porous support and anion exchange groups attached thereto. A first surface and a second surface are formed on both sides of the porous support. The anion exchange groups include strong anion exchange groups and weak anion exchange groups. The strong anion exchange groups are quaternary ammonium groups, and the weak anion exchange groups are one or more of primary, secondary, and tertiary amine groups. The nitrogen content of the first surface of the anion exchange chromatography membrane is in the ratio of nitrogen from the strong anion exchange groups to nitrogen from the weak anion exchange groups at (1-5):1. This invention, by controlling the inclusion of both strong and weak anion exchange groups and limiting their ratio, results in anion exchange chromatography membranes with high cycle life and good purification effects when used for monoclonal antibody purification.

The invention provides a nanofiber aerogel material for strong anion exchange and a preparation method of the nanofiber aerogel material. The preparation method comprises the following steps: firstly, co-crosslinking PVA-co-PE nanofibers and polyethyleneimine to obtain nanofiber aerogel, then immersing the aerogel material in a non-polar solvent solution of trimesoyl chloride for interface reaction, then drying, then immersing in a hydroxyl quaternary ammonium salt aqueous solution for grafting quaternary ammonium groups, and finally drying to obtain the PVA-co-PE nanofiber aerogel. And finally, obtaining the nanofiber aerogel material for strong anion exchange. Amino functional groups of PEI and acyl chloride groups of TMC are subjected to interface reaction, and then quaternary ammonium groups are grafted on the surface. The PEI / PVA-co-PE composite aerogel has abundant pore channels, and meanwhile, PEI has abundant primary amine and tertiary amine groups and is grafted with a large number of quaternary ammonium groups, so that the ionic strength of the aerogel material is greatly improved, the anion exchange performance is improved, and a selective adsorption function is realized. The nanofiber aerogel material is high in adsorption efficiency, large in adsorption capacity and good in repeatability.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

The present application belongs to the field of bioengineering technology, and particularly relates to a culture method of PDRN genetically engineered cells, a product purification method and application. The present application solves the problems of low yield, growth-synthesis imbalance and poor purification effect in the production of existing recombinant PDRN. During the culture, the fermentation medium is optimized, the nucleotide metabolic pathway is up-regulated in cooperation with a low-dose PDRN synthesis precursor and a metabolic activator, and the pH and dissolved oxygen of the fermentation are controlled in stages to maintain the balance between the growth of the bacterial cells and the synthesis of the product. During the purification, the fermentation broth is pretreated, then subjected to microfiltration, ultrafiltration, Q-agarose gel FF strong anion exchange chromatography and freeze-drying. The present application improves the yield of PDRN, and the purity and yield of the finished product are high, which is suitable for industrial production. The obtained PDRN can be used in the fields of biomaterials, medicine, medical aesthetics and cosmetics.