34 results about "Stepwise elution" patented technology

The invention discloses a method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves. The method comprises a macro-porous resincolumn adsorption process, an eluent stepwise elution process and a gel chromatographic column refining process. The Poacynum hendersonii leaves are used as a raw material, and are processed to obtainPoacynum hendersonii leaf extract, the extract is fully dissolved in distilled water, insoluble substances are filtered out, the obtained filtrate goes through a macro-porous resin column and undergoes stepwise elution with an eluent, and the obtained first-stage eluate is concentrated and freeze-dried to obtain chlorogenic acid having a purity of 52% or above; and the obtained second-stage eluate undergoes reduced pressure distillation, and the obtained product is dissolved in methanol, and is continuously purified by a gel chromatographic column to obtain the quercetin-3-O-sophoroside having a purity of 93% or above and isoquercetin having a purity of 97% or above. The above separation method allows highly pure quercetin-3-O-sophoroside (93% or above), isoquercetin (97% or above ) and chlorogenic acid (52% or above) to be simultaneously obtained in a simple process, so the method has the advantages of simple and feasible process, high production efficiency, high raw material utilization rate, suitableness for industrial production, and high production and practical values.