The application discloses a
benzofuran glycoside compound, a separation and extraction method thereof, a pharmaceutical composition and application, and the compound 1 is separated from a
macleaya cordata extract. A
benzofuran compound is obtained by separating and purifying a n-
butanol part chemical component of a
macleaya cordata root. The inhibitory activity of all separated compounds 1 on a target point
cyclooxygenase-2 of an inflammatory related
disease is tested, the compound 1 has good inhibitory activity on COX-2
enzyme, and the compound 1 has better
binding energy with the target point
protein. The compound also has good NLRP3 related
inhibitory effect, can be used alone or in combination with other drugs to regulate NLRP3 related proteins, is used for treating NLRP3 related mediated diseases and / or diseases, and is used for preparing drugs for preventing or treating the diseases. The compound 1 also has better
inhibitory effect on
acetylcholinesterase, and can be used for treating diseases such as Alzheimer's
disease,
myasthenia gravis and Parkinson's
disease.