342 results about "Diterpene" patented technology

The invention belongs to the technical application field of microbial metabolic engineering and synthetic biology, and particularly relates to a pichia pastoris engineering strain for high-yield sclareol as well as a construction method and application of the pichia pastoris engineering strain. The method comprises the following steps: constructing a biosynthetic pathway of sclareol in a host strain, and optimizing an intracellular mevalonic acid metabolic pathway and a central metabolic pathway to obtain an engineering strain A; the host strain is pichia pastoris; or overexpressing or knocking out a metabolism regulation factor in the obtained engineering bacterium A to obtain an engineering bacterium strain B; or, in the obtained engineering bacterium B, a synthetic pathway is targeted to peroxisome and optimized by adopting a cell region compartment strategy, and an engineering bacterium strain C is obtained. The invention provides a pichia pastoris chassis cell for synthesizing a diterpenoid sclareol and an engineering strain for synthesizing sclareol. By introducing and optimizing the expression of a sclareol synthesis pathway, the fermentation yields of sclareol in shake flask batch and bioreactor batch fed-batch fermentation are respectively 631.6 mg / L and 10.5 g / L.

The invention relates to the technical field of high polymer materials, and discloses rosin-based bio-based ultraviolet curing resin as well as a preparation method and application thereof. The structural formula of the rosin-based bio-based ultraviolet curing resin provided by the invention is shown as a formula (I). The ultraviolet curing resin has a tricyclic diterpene rigid structure and carbon-carbon double bonds, has good crosslinking density, adhesion property, mechanical property and thermal stability after being cured into a film, and can be applied to the fields of adhesives, sealants or ultraviolet curing coatings and the like. The ultraviolet curing resin is prepared by taking a bio-based material as a raw material, and the preparation method is simple, green and environment-friendly. # imgabs0 #

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to the technical field of pet food antioxidants, in particular to a compound pet food antioxidant containing plant polyphenol compounds and a preparation method of the compound pet food antioxidant. 10 to 20 parts of a grape seed extract; 8 to 15 parts of a rosemary extract; 5 to 10 parts of L-ascorbyl palmitate; 5 to 10 parts of alpha-tocopheryl acetate; 3-8 parts of citric acid; 8 to 15 parts of soybean lecithin; 20 to 30 parts of medium chain triglyceride; eGCG (epigallocatechin gallate) in the green tea contains a plurality of o-dihydroxyl structures, the grape seed procyanidine has a unique C6-C3-C6 skeleton, and carnosic acid in the rosemary belongs to a diterpenoid compound. Due to the diversity of the structure, the compound can play a role in different types of free radicals and oxidation reactions, and a broad-spectrum anti-oxidation network is formed.

Provided are: a biofilm formation inhibitor that can inhibit the formation of a biofilm even when used at a low concentration at which antibacterial activity is not exhibited; a method for inhibiting the formation of a biofilm; a resin composition; a molded article; and a coating agent. The biofilm formation inhibitor comprises a diterpene compound (A) having an octanol / water partition coefficient (logP) of 6 or more.

The invention provides a euphorbia lathyris acyltransferase gene ElBAHD16 and application of a coding protein thereof. The gene is obtained by cloning from euphorbia lathyris for the first time. The invention also provides a recombinant vector and an engineering bacterium containing the gene. Cinnamoyl coenzyme A, benzoyl coenzyme A or acetyl coenzyme A can be used as an acyl donor, and 7-hydroxy euphorbia lathyris diterpenoid alcohol is used as a substrate to generate a plurality of types of euphorbia lathyris diterpenoid esters through biological catalysis. The content of diterpenoid ester substances in plants such as euphorbia lathyris can be increased through a genetic engineering technology, and the method has a good application prospect.

The invention belongs to the field of genetic engineering and synthetic biology, and provides a novel cytochrome P450 enzyme derived from cephalotaxus plants, a nucleotide, a carrier and a host of the cytochrome P450 enzyme, and application of the cytochrome P450 enzyme in preparation of cephalotaxane diterpenoid compounds. The cytochrome P450 enzyme provided by the invention has multiple catalytic functions, can efficiently catalyze oxidation of C-5 site and C-19 site of cephalotaxene, lays a solid foundation for analysis of a cephalotaxane diterpene biosynthetic pathway, and provides a new method for obtaining important active molecules such as cephalotaxus hainanensis lactone and the like by adopting a synthetic biological means.

The invention discloses a preparation method and application of a novel bird nest alkane diterpenoid compound, and relates to the technical field of medicinal chemistry. The invention aims to solve the problems that chemical synthesis needs harsh conditions and the cost is difficult to control when diterpenoid compounds are obtained in the prior art, and a large amount of resources are wasted and new molecules are difficult to obtain under conventional fermentation conditions. The prepared bird nest alkane type diterpene has dual functions of promoting neurite growth and nourishing neurons, is expected to be developed into a neuroprotective agent, and is used for treating neurodegenerative diseases and peripheral nerve injury diseases; meanwhile, the problems that in the prior art, when the compounds are obtained, conditions needed by chemical synthesis are harsh, the cost is difficult to control, a large number of resources are wasted under conventional fermentation conditions, but new molecules are difficult to obtain, and production and smooth follow-up biological activity research are not facilitated are effectively solved. The invention provides the preparation method and the application of the novel bird nest alkane diterpenoid compound.

The invention relates to the technical field of medicines, in particular to application of lasiokaurin and cis-platinum combined medicine in preparation of a medicine for treating liver cancer. As a diterpenoid compound, lasiokaurin shows significant potential in the anti-cancer field. However, in medical research and clinical application at present, related reports about combined use of lasiokaurin and a classical anti-cancer drug cis-platinum for treating liver cancer do not exist. Based on the current research situation, the invention creatively provides the application of the lasiokaurin and cis-platinum combined medicine in the aspect of preparing the liver cancer treatment medicine. In-depth study finds that lasiokaurin and cis-platinum can generate a synergistic effect and jointly play a strong anti-tumor role, and a brand new way is opened up for treatment of liver cancer.

The invention discloses a microbial chassis cell capable of producing terpenoids at high yield and application of the microbial chassis cell. Based on gene homologous recombination of a specific site mediated by a conjugational transfer technology, a key rate-limiting enzyme gene of an MEP pathway is overexpressed in an integrated manner at a gene high expression site in a genome, streptomyces chassis cells with high yield of terpenes are established, and establishment of an efficient production system of sesquiterpenes and diterpenes is realized. By utilizing the microorganism provided by the embodiment of the invention, the yields of bisabolol, 18-carboxyl labdate, kaurene and atelonic acid are obviously improved, the production period is short, and the production efficiency is high.

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for producing lycopene as well as a system construction method and application thereof, the engineering bacterium for producing lycopene is escherichia coli and carries two plasmids, namely a pCDF-Duet-MKI4 plasmid and a pET28a-crtBI plasmid; a pCDF-Duet plasmid is adopted to express an MKI4 gene, a five-carbon compound substrate is converted into a diterpene precursor geranyl geranyl pyrophosphate (GGPP), a pET28a plasmid is adopted to express crtB and crtI genes, the GGPP is converted into phytoene, and the phytoene is further converted into lycopene. According to the invention, a biosynthesis system of lycopene is constructed in Escherichia coli, a five-carbon compound substrate can be efficiently converted into lycopene, and the shake flask fermentation yield can reach up to 900 mg / L.

The invention discloses a cadinane type sesquiterpene as well as a preparation method and application thereof. The cadinane type sesquiterpene is separated from cinnamon bark, and has at least one of structural formulas shown as a formula (I), a formula (II), a formula (III) and a formula (IV). Liver protection activity evaluation is carried out by applying an alcohol-induced hepatocyte injury model, and it is found that the cadinane type sesquiterpene can effectively relieve alcohol-induced AML-12 hepatocyte injury; the dumarane sesquiterpenes can inhibit the alcoholic liver disease and relieve fat accumulation, so that the dumarane sesquiterpenes have the anti-alcoholic liver disease activity, can be applied to preparation of the anti-alcoholic liver disease drugs, and have good research and development prospects; belongs to the field of natural medicinal chemistry.

The present invention belongs to the field of microbial metabolic engineering and synthetic biology technology applications, and particularly relates to a Pichia pastoris engineered strain that produces high sclareol production, as well as its construction method and application. A sclareol biosynthesis pathway is constructed in a host strain, and the intracellular mevalonate metabolic pathway and the central metabolic pathway are optimized, thereby obtaining an engineered bacterial strain A; the host strain is Pichia pastoris; or, metabolic regulatory factors are overexpressed or knocked out in the above-obtained engineered bacterial strain A, thereby obtaining an engineered bacterial strain B; or, in the above-obtained engineered bacterial strain B, a cell compartmentalization strategy is used to target the synthesis pathway to the peroxisome and optimize it, thereby obtaining an engineered bacterial strain C. The present invention provides a Pichia pastoris chassis cell for synthesizing the diterpenoid compound sclareol and an engineered strain for synthesizing sclareol; by introducing and optimizing the expression of the sclareol synthesis pathway, the sclareol yields in shake flask batch fermentation and bioreactor batch fed-batch fermentation reach 631.6 mg / L and 10.5 g / L, respectively.

The invention discloses a method for efficiently inducing hairy roots of begonia for producing ingenol. The obtained hairy roots can be used for extracting an active substance ingenol with the content higher than that in an original plant. The method comprises the following steps: 1) preparing a sterile explant; (2) activating agrobacterium and preparing a bacterium solution; (3) inducing hairy roots; (4) carrying out degerming induction culture; (5) subculture; and 6) amplification culture of hairy roots. By applying the induction culture method disclosed by the invention, efficient induction and large-scale culture of the euphorbia hairy roots can be realized, and the hairy roots of the euphorbia which grow quickly, are stable and good in state and contain a large amount of ingenol are obtained; the technical support is provided for the production of the active euphorbia pekinensis diterpenoid compound of the medicinal plant, namely the lepidogramme, and the development and utilization of medicinal resources.

The invention relates to a composition for use in the oral treatment of nemathelminthiasis, said composition comprising eleganolone and / or a bifurcanol-type linear diterpene, and a process for preparing a corresponding anthelmintic.

The invention relates to application of a diterpene derivative ST-4 and a sodium salt ST-4Na thereof, the compound is ST-4 or the sodium salt ST-4Na thereof, activation of NLRP3 in microglial cells can be inhibited, neuroinflammation activated and mediated by the microglial cells is further inhibited, loss of dopaminergic neurons induced by MPTP can be reversed, and the effect of inhibiting neuroinflammation is achieved. The apoptosis of dopaminergic neurons is reduced, so that the effect of treating Parkinson's disease is achieved. The treatment effect on the Parkinson's disease is equivalent to or even better than that of an existing medicine L-Dopa, other neuronal cells cannot be damaged, and the L-Dopa can be applied to preparation of the medicine for treating the neurodegenerative disease, especially the medicine for treating the Parkinson's disease.

The present application relates to the technical field of microbial medicine, and particularly to a preparation method and application of indole diterpenoid compounds. Specifically, the extract of plant endophytic fungus F4a after solid fermentation culture is separated and purified to obtain indole diterpenoid compounds; the plant endophytic fungus F4a is a strain with the preservation number CCTCC NO: M 2012531. The indole diterpenoid compounds provided by the present application have significant antifeedant, insecticidal and hypotensive activities, and they can be used as ideal candidate compounds of antifeedants, insecticides or hypoglycemic drugs. The compounds can be produced through fungal fermentation, and the preparation method is simple, efficient and high in product purity. The present application has good application prospect.

The present invention discloses a method of treatment and / or prevention of obesity using a composition comprising of brominated diterpenes from red algae of the genus Laurencia as active ingredients and preferably the diterpene neorogioldiol, isolated from Laurencia sp. or chemically synthesized. The said diterpenes suppress pre-adipocyte differentiation to adipocytes, modulate metabolism in 3T3-L1 cells, restrict weight gain and development of glucose intolerance when administered intraperitoneally in vivo, in the mouse model of high fat diet-induced obesity.

The invention discloses a polyfunctional rosin-based reactive diluent. The structural formula of the polyfunctional rosin-based reactive diluent is shown as a formula (I) or a formula (II). The polyfunctional rosin-based reactive diluent has a rigid tricyclic diterpenoid structure of rosin, is small in molecular weight and low in viscosity, can be used for preparing an ultraviolet curing material, has excellent hardness and scratch resistance after light curing and film forming, and is simple in preparation method, low in cost and suitable for industrial production. And a polycarboxylic acid functional group is efficiently constructed under a mild condition.

To provide an andrographis paniculata diterpene lactone compound as well as extraction method and application thereof.SOLUTION: The andrographis paniculata diterpene lactone compound provided by the invention has a structure as shown in any one of formulas 1-7 and is novel in structure. The andrographis paniculata diterpene lactone compound extracted and separated from andrographis paniculata has a good anti-inflammatory effect, and has a good down-regulation effect on secretion of NO, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a lipopolysaccharide (LPS) induced mouse mononuclear macrophage (RAW264.7 cell) inflammation model; the compound also has a good inhibition effect on expression of inducible nitric oxide synthase (INOS) and cyclooxygenase-2 (COX-2) proteins, and can be applied to preparation of anti-inflammatory drugs.SELECTED DRAWING: Figure 1

The application relates to the medical technical field, in particular to application of neoline in treatment of Parkinson's disease. 19 The C 19 The type-II diterpene alkaloid neoline can significantly improve movement disorder in a MPTP-induced Parkinson's disease model mouse, the drug efficacy is equivalent to that of the positive drug madopar, the effective dose is much lower than that of the positive drug, the administration mode is oral administration, drug compliance and use convenience are good, and the neoline can be used for development of anti-Parkinson's disease drugs.

The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a parent structure of a PKM2 agonist and application of the PKM2 agonist, the PKM2 agonist is hypaconitine screened from a monkshood alkaloid part, the chemical formula of the PKM2 agonist is C33H45NO10, and the structural formula of the PKM2 agonist is shown in the description. The hypaconitine has the functions of inhibiting phosphorylation of tumor cell PKM2 protein, inhibiting conversion from tetramer to dimer, enhancing PKM2 activity and inhibiting PKM2 nuclear translocation; the parent structure of the hypaconitine is composed of diterpenoid skeleton atoms, and the parent nucleus of the hypaconitine is C19 diterpenoid alkaloid; according to the invention, a PKM2 activating component in monkshood alkaloid is screened by using computer-aided drug design, and it is reported for the first time that hypaconitine has a PKM2 activating effect; a certain foundation is laid for the research on the anti-tumor mechanism of the radix aconiti carmichaeli, a basis is provided for the clinical application of the anti-tumor of the radix aconiti carmichaeli, and a new parent structure is provided for the research and development of a PKM2 agonist.

The application discloses application of an NtBCP gene in synthesis of cembranoid diterpenoids in tobacco and improvement of tobacco germplasm resources and belongs to the technical field of tobacco genetic engineering. The application proves through experiments that the NtBCP gene is specifically expressed in tobacco trichome stalk cells and is highly related to metabolism of cembranoid diterpenoids in tobacco, and the nucleotide sequence of the NtBCP gene is shown as SEQ ID NO. 1. Further through overexpression technology, the application finds that after overexpression of the NtBCP gene in cultivated tobacco, the obtained transgenic tobacco has obviously increased cembranoid diterpenoids compared with normal control plants. The application can provide a new strategy and path for genetic engineering breeding of tobacco or cultivation of new varieties of other crops.

The present invention refers to a method for the preparation of a compound according to formula (45), its enantiomers, diastereomers, a racemic mixture, and the pharmaceutically acceptable salts thereof. Furthermore the invention refers to a compound of formula (45) prepared by said method for use in treatment of neurodegenerative disorders and to pharmaceutical compositions.

The present invention discloses terpene compounds derived from polar fungi, their preparation methods and applications, and respectively provides a calamenane-type sesquiterpene compound eutyacorane and a pimarane-type diterpene compound libertellenone Z derived from polar fungi. The structures are shown in the following formulas I and II respectively: The experimental results show that the two compounds have a better inhibition rate on the release of NO in the LPS-induced inflammatory model of mouse macrophages RAW264.7, providing a new type of choice for anti-inflammatory drugs.

The invention relates to the technical field of medicines, in particular to a composition with ingenane diterpenoid compounds as chemotherapeutic drug sensitizers and application of the composition. The chemotherapeutic drug sensitizer and the composition thereof provided by the invention can be used as the chemotherapeutic drug sensitizer, especially the sensitizer of breast cancer treatment drugs, and can be used for increasing the sensitivity of breast cancer cells to the chemotherapeutic drugs by inhibiting CHK1 (Ser345) phosphorylation so as to realize sensitization and provide a new treatment strategy for treating breast cancer.

The application belongs to the field of genetic engineering and biosynthesis, and particularly relates to biosynthesis of a class of diterpenoids. The application provides genes having a key role in the formation of a 5-8-5 tricyclic mother nucleus and a five-membered heterocyclic ring in biosynthesis of the class of diterpenoids, which are respectively named as PrcA and PrcB and polypeptides encoded by the genes, expression of PrcA and PrcB is carried out through a heterologous expression system of Aspergillus oryzae, and five diterpenoids with novel structures and anti-metabolic related fatty liver disease activity are obtained. The application provides application of the polypeptides in biosynthesis of the class of diterpenoids, and enriches a diterpenoid library, thereby providing lead compound resources for discovering new anti-metabolic related fatty liver disease medicinal raw materials.

The invention discloses an application of ultrasound and teuvincenone H diterpenoid treatment in reduction of tenebrio molitor protein allergy, the tenebrio molitor protein is treated by ultrasound and teuvincenone H diterpenoid, so that the binding capacity of the tenebrio molitor protein and immunoglobulin E (IgE) can be reduced, and the allergy of the tenebrio molitor protein is further reduced. The invention discloses other effects of teuvincenone H diterpene except for anti-tumor and anti-inflammatory effects for the first time, and the method has the advantages of simple process steps, short treatment time, low temperature, high efficiency, rapidness and environmental friendliness, is beneficial to wide application of related technologies in the field of food industry, and provides a brand new scheme for developing safe and high-quality insect protein food.

The invention discloses a forsythia alkaloid B and its preparation method and anti-inflammatory and antiviral applications, belonging to the technical field of pharmaceutical chemistry. The forsythia alkaloid B is a norcisan-type diterpene alkaloid with a molecular formula of C 17 H 23 NO2, the structural formula is as follows. The invention is beneficial in that: the forsythia alkaloid B is separated from the fruit of forsythia by an innovative extraction and separation process, the forsythia alkaloid B has a novel structure, and has good anti-inflammatory and anti-H9N2, H5N1 and H1N1 influenza virus activities, providing a new material basis for the research on the anti-inflammatory and antiviral activities of forsythia. #imgabs0#Forsythia alkaloid B.