284 results about "Diterpene" patented technology

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to the technical field of pet food antioxidants, in particular to a compound pet food antioxidant containing plant polyphenol compounds and a preparation method of the compound pet food antioxidant. 10 to 20 parts of a grape seed extract; 8 to 15 parts of a rosemary extract; 5 to 10 parts of L-ascorbyl palmitate; 5 to 10 parts of alpha-tocopheryl acetate; 3-8 parts of citric acid; 8 to 15 parts of soybean lecithin; 20 to 30 parts of medium chain triglyceride; eGCG (epigallocatechin gallate) in the green tea contains a plurality of o-dihydroxyl structures, the grape seed procyanidine has a unique C6-C3-C6 skeleton, and carnosic acid in the rosemary belongs to a diterpenoid compound. Due to the diversity of the structure, the compound can play a role in different types of free radicals and oxidation reactions, and a broad-spectrum anti-oxidation network is formed.

The invention belongs to the field of genetic engineering and synthetic biology, and provides a novel cytochrome P450 enzyme derived from cephalotaxus plants, a nucleotide, a carrier and a host of the cytochrome P450 enzyme, and application of the cytochrome P450 enzyme in preparation of cephalotaxane diterpenoid compounds. The cytochrome P450 enzyme provided by the invention has multiple catalytic functions, can efficiently catalyze oxidation of C-5 site and C-19 site of cephalotaxene, lays a solid foundation for analysis of a cephalotaxane diterpene biosynthetic pathway, and provides a new method for obtaining important active molecules such as cephalotaxus hainanensis lactone and the like by adopting a synthetic biological means.

The invention discloses a preparation method and application of a novel bird nest alkane diterpenoid compound, and relates to the technical field of medicinal chemistry. The invention aims to solve the problems that chemical synthesis needs harsh conditions and the cost is difficult to control when diterpenoid compounds are obtained in the prior art, and a large amount of resources are wasted and new molecules are difficult to obtain under conventional fermentation conditions. The prepared bird nest alkane type diterpene has dual functions of promoting neurite growth and nourishing neurons, is expected to be developed into a neuroprotective agent, and is used for treating neurodegenerative diseases and peripheral nerve injury diseases; meanwhile, the problems that in the prior art, when the compounds are obtained, conditions needed by chemical synthesis are harsh, the cost is difficult to control, a large number of resources are wasted under conventional fermentation conditions, but new molecules are difficult to obtain, and production and smooth follow-up biological activity research are not facilitated are effectively solved. The invention provides the preparation method and the application of the novel bird nest alkane diterpenoid compound.

The invention relates to the technical field of medicines, in particular to application of lasiokaurin and cis-platinum combined medicine in preparation of a medicine for treating liver cancer. As a diterpenoid compound, lasiokaurin shows significant potential in the anti-cancer field. However, in medical research and clinical application at present, related reports about combined use of lasiokaurin and a classical anti-cancer drug cis-platinum for treating liver cancer do not exist. Based on the current research situation, the invention creatively provides the application of the lasiokaurin and cis-platinum combined medicine in the aspect of preparing the liver cancer treatment medicine. In-depth study finds that lasiokaurin and cis-platinum can generate a synergistic effect and jointly play a strong anti-tumor role, and a brand new way is opened up for treatment of liver cancer.

The invention discloses a cadinane type sesquiterpene as well as a preparation method and application thereof. The cadinane type sesquiterpene is separated from cinnamon bark, and has at least one of structural formulas shown as a formula (I), a formula (II), a formula (III) and a formula (IV). Liver protection activity evaluation is carried out by applying an alcohol-induced hepatocyte injury model, and it is found that the cadinane type sesquiterpene can effectively relieve alcohol-induced AML-12 hepatocyte injury; the dumarane sesquiterpenes can inhibit the alcoholic liver disease and relieve fat accumulation, so that the dumarane sesquiterpenes have the anti-alcoholic liver disease activity, can be applied to preparation of the anti-alcoholic liver disease drugs, and have good research and development prospects; belongs to the field of natural medicinal chemistry.

The present invention belongs to the field of microbial metabolic engineering and synthetic biology technology applications, and particularly relates to a Pichia pastoris engineered strain that produces high sclareol production, as well as its construction method and application. A sclareol biosynthesis pathway is constructed in a host strain, and the intracellular mevalonate metabolic pathway and the central metabolic pathway are optimized, thereby obtaining an engineered bacterial strain A; the host strain is Pichia pastoris; or, metabolic regulatory factors are overexpressed or knocked out in the above-obtained engineered bacterial strain A, thereby obtaining an engineered bacterial strain B; or, in the above-obtained engineered bacterial strain B, a cell compartmentalization strategy is used to target the synthesis pathway to the peroxisome and optimize it, thereby obtaining an engineered bacterial strain C. The present invention provides a Pichia pastoris chassis cell for synthesizing the diterpenoid compound sclareol and an engineered strain for synthesizing sclareol; by introducing and optimizing the expression of the sclareol synthesis pathway, the sclareol yields in shake flask batch fermentation and bioreactor batch fed-batch fermentation reach 631.6 mg / L and 10.5 g / L, respectively.

The present application relates to the technical field of microbial medicine, and particularly to a preparation method and application of indole diterpenoid compounds. Specifically, the extract of plant endophytic fungus F4a after solid fermentation culture is separated and purified to obtain indole diterpenoid compounds; the plant endophytic fungus F4a is a strain with the preservation number CCTCC NO: M 2012531. The indole diterpenoid compounds provided by the present application have significant antifeedant, insecticidal and hypotensive activities, and they can be used as ideal candidate compounds of antifeedants, insecticides or hypoglycemic drugs. The compounds can be produced through fungal fermentation, and the preparation method is simple, efficient and high in product purity. The present application has good application prospect.

The present invention discloses a method of treatment and / or prevention of obesity using a composition comprising of brominated diterpenes from red algae of the genus Laurencia as active ingredients and preferably the diterpene neorogioldiol, isolated from Laurencia sp. or chemically synthesized. The said diterpenes suppress pre-adipocyte differentiation to adipocytes, modulate metabolism in 3T3-L1 cells, restrict weight gain and development of glucose intolerance when administered intraperitoneally in vivo, in the mouse model of high fat diet-induced obesity.

The invention discloses a polyfunctional rosin-based reactive diluent. The structural formula of the polyfunctional rosin-based reactive diluent is shown as a formula (I) or a formula (II). The polyfunctional rosin-based reactive diluent has a rigid tricyclic diterpenoid structure of rosin, is small in molecular weight and low in viscosity, can be used for preparing an ultraviolet curing material, has excellent hardness and scratch resistance after light curing and film forming, and is simple in preparation method, low in cost and suitable for industrial production. And a polycarboxylic acid functional group is efficiently constructed under a mild condition.

To provide an andrographis paniculata diterpene lactone compound as well as extraction method and application thereof.SOLUTION: The andrographis paniculata diterpene lactone compound provided by the invention has a structure as shown in any one of formulas 1-7 and is novel in structure. The andrographis paniculata diterpene lactone compound extracted and separated from andrographis paniculata has a good anti-inflammatory effect, and has a good down-regulation effect on secretion of NO, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a lipopolysaccharide (LPS) induced mouse mononuclear macrophage (RAW264.7 cell) inflammation model; the compound also has a good inhibition effect on expression of inducible nitric oxide synthase (INOS) and cyclooxygenase-2 (COX-2) proteins, and can be applied to preparation of anti-inflammatory drugs.SELECTED DRAWING: Figure 1

The application relates to the medical technical field, in particular to application of neoline in treatment of Parkinson's disease. 19 The C 19 The type-II diterpene alkaloid neoline can significantly improve movement disorder in a MPTP-induced Parkinson's disease model mouse, the drug efficacy is equivalent to that of the positive drug madopar, the effective dose is much lower than that of the positive drug, the administration mode is oral administration, drug compliance and use convenience are good, and the neoline can be used for development of anti-Parkinson's disease drugs.

The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

The application discloses application of an NtBCP gene in synthesis of cembranoid diterpenoids in tobacco and improvement of tobacco germplasm resources and belongs to the technical field of tobacco genetic engineering. The application proves through experiments that the NtBCP gene is specifically expressed in tobacco trichome stalk cells and is highly related to metabolism of cembranoid diterpenoids in tobacco, and the nucleotide sequence of the NtBCP gene is shown as SEQ ID NO. 1. Further through overexpression technology, the application finds that after overexpression of the NtBCP gene in cultivated tobacco, the obtained transgenic tobacco has obviously increased cembranoid diterpenoids compared with normal control plants. The application can provide a new strategy and path for genetic engineering breeding of tobacco or cultivation of new varieties of other crops.

The present invention refers to a method for the preparation of a compound according to formula (45), its enantiomers, diastereomers, a racemic mixture, and the pharmaceutically acceptable salts thereof. Furthermore the invention refers to a compound of formula (45) prepared by said method for use in treatment of neurodegenerative disorders and to pharmaceutical compositions.

The present invention discloses terpene compounds derived from polar fungi, their preparation methods and applications, and respectively provides a calamenane-type sesquiterpene compound eutyacorane and a pimarane-type diterpene compound libertellenone Z derived from polar fungi. The structures are shown in the following formulas I and II respectively: The experimental results show that the two compounds have a better inhibition rate on the release of NO in the LPS-induced inflammatory model of mouse macrophages RAW264.7, providing a new type of choice for anti-inflammatory drugs.

The invention relates to the technical field of medicines, in particular to a composition with ingenane diterpenoid compounds as chemotherapeutic drug sensitizers and application of the composition. The chemotherapeutic drug sensitizer and the composition thereof provided by the invention can be used as the chemotherapeutic drug sensitizer, especially the sensitizer of breast cancer treatment drugs, and can be used for increasing the sensitivity of breast cancer cells to the chemotherapeutic drugs by inhibiting CHK1 (Ser345) phosphorylation so as to realize sensitization and provide a new treatment strategy for treating breast cancer.

The application belongs to the field of genetic engineering and biosynthesis, and particularly relates to biosynthesis of a class of diterpenoids. The application provides genes having a key role in the formation of a 5-8-5 tricyclic mother nucleus and a five-membered heterocyclic ring in biosynthesis of the class of diterpenoids, which are respectively named as PrcA and PrcB and polypeptides encoded by the genes, expression of PrcA and PrcB is carried out through a heterologous expression system of Aspergillus oryzae, and five diterpenoids with novel structures and anti-metabolic related fatty liver disease activity are obtained. The application provides application of the polypeptides in biosynthesis of the class of diterpenoids, and enriches a diterpenoid library, thereby providing lead compound resources for discovering new anti-metabolic related fatty liver disease medicinal raw materials.

The invention discloses a plant extract for killing pests and a preparation process of the plant extract, and relates to the technical field of biopesticides, and the technical key points are as follows: the plant extract is selected from one or more of adhatoda vasica Nees, azadirachta indica, nigra sativa, parthenium hysterium and oriental arborvitae Thuja orientia, and the extract contains phytosterol, saponin, diterpene, flavone and alkaloid. Contact and residual toxicity tests are carried out on the cigarette beetles cultured in a laboratory, the experimental result shows that the neem and adhatoda vasica extracts have remarkable toxicity and repelling effects on the cigarette beetles, the neem has the highest toxicity, and the LC50 value is the lowest. The method comprises the steps of plant collection, drying, grinding, solvent extraction, rotary evaporation and the like so as to obtain high-concentration active ingredients. The plant extract of the invention can be incorporated into the manufacture of novel biological insecticides to reduce the harm of cigarette beetles.

The invention relates to the field of natural medicines. In particular to a novel skeleton compound spirolathyrisin A which is obtained by taking roots of euphorbia lathyris as a raw material and extracting and separating through a natural product chemical method, the skeleton of the spirolathyrisin A belongs to euphorbia lathyris spiro-diterpenoid, and the spirolathyrisin A has a relatively strong inhibition effect on plant pathogenic fungi and can provide a novel lead compound for screening of prevention and treatment of plant fungal diseases.

The invention discloses a metabolic pathway of a clerodane type diterpenoid compound. The metabolic pathway is characterized in that related metabolic genes in the biosynthesis process of annonene, hardwickic acid, hatriwaic acid and divinatorin A are analyzed. The invention further discloses a preparation method of the clerodane type diterpenoid compound, and application of the clerodane type diterpenoid compound in the biosynthesis process of the clerodane type diterpenoid compound in the biosynthesis process of the clerodane type diterpenoid compound. The invention also verifies the function of some cytochrome P450 enzymes in the aspect of producing the clerodane diterpenoid compounds by utilizing the fermentation of yeast engineering bacteria, and lays a foundation for the industrialization of the clerodane diterpenoid compounds and intermediates of the clerodane diterpenoid compounds.

The present invention provides a method for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, the method comprising modifying the tobacco plant or the tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides the use of a diterpene synthesis gene for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, and tobacco cells, tobacco plants, tobacco plant propagation materials, leaves harvested, processed tobacco or tobacco products obtainable according to the present invention.

The present invention discloses a method of treatment and / or prevention of obesity using a composition comprising of brominated diterpenes from red algae of the genus Laurencia as active ingredients and preferably the diterpene neorogioldiol, isolated from Laurencia sp. or chemically synthesized. The said diterpenes suppress pre-adipocyte differentiation to adipocytes, modulate metabolism in 3T3-L1 cells, restrict weight gain and development of glucose intolerance when administered intraperitoneally in vivo, in the mouse model of high fat diet-induced obesity.

This invention discloses an isosporanoid diterpenoid compound, its pharmaceutical composition, its preparation method, and its application, relating to the field of pharmaceutical technology. This invention is the first to extract the isosporanoid diterpenoid compound 3-epi-kravanhin A from the stems and leaves of *Amomum villosum*, and the structure of this isosporanoid diterpenoid is a novel compound reported for the first time. The isosporanoid diterpenoid compound 3-epi-kravanhin A isolated from the stems and leaves of *Amomum villosum* exhibits significant GLP-1 secretion-promoting and DPP-4-inhibiting activities. Experiments show that compound 3-epi-kravanhin A has hypoglycemic and weight-loss effects.

The present invention provides a tricyclic diterpenoid compound having anti-influenza virus activity. The tricyclic diterpenoid compound not only has better anti-H1N1 virus activity, but also is low in toxicity. The invention also discloses a preparation method of the tricyclic diterpenoid compound with antiviral activity. The preparation method comprises the following steps: (1) obtaining a fermentation product rich in the tricyclic diterpenoid compound through microbial fermentation culture; a recombinant vector adopted in the microbial fermentation culture carries nucleotide sequences: PQ793156 (dioxygenase gene), PQ793157 (FAD dependent monooxygenase gene), PQ793158 (terpenoid synthase gene), PQ793159 (UbiA family isopentenyl transferase gene), PQ793160 (GGPP synthase gene) and PQ793161 (amino transferase gene). And (2) separating and purifying the fermentation product obtained in the step (1) to obtain the tricyclic diterpenoid compound.

The present invention relates to diterpenoid alkaloid compounds in Delphinium aemulans Nevski var. aemulans and a preparation method and use thereof. The compounds use Delphinium aemulans Nevski var. aemulans as a raw material, are extracted with a solvent, treated with acid and base, subjected to solvent extraction, and separated by two, three or four of silica gel column chromatography, Sephadex LH-20 column chromatography, preparative thin layer chromatography or high performance liquid chromatography, and detected and analyzed by thin layer chromatography or high performance liquid chromatography to obtain two new compounds: Compound 1 named aemulanosine A; Compound 2 named aemulanosine B. The voltage-gated potassium channel activities of the two compounds were measured. The experimental results show that aemulanosine A and aemulanosine B isolated from Delphinium aemulans Nevski var. aemulans show inhibitory effects on voltage-gated potassium channel currents and can be used for the preparation of drugs with the activity of inhibiting voltage-gated potassium channel currents.

The invention relates to the technical field of medicines, in particular to an enantiomer-atesane type diterpenoid compound as well as a preparation method and an application of the enantiomer-atesane type diterpenoid compound. The enantiomer-atesane diterpenoid compound disclosed by the invention is derived from plants, has anti-tumor activity, has cytotoxicity to human leukemia cells, human liver cancer cells and human cervical cancer cells, and is relatively good in inhibitory activity. Wherein the compound 3 has the best effect, the IC50 values of the compound 3 to three human tumor cells are 7.5 + / -1.2 micromole per liter, 9.8 + / -1.0 micromole per liter and 10.1 + / -0.9 micromole per liter respectively, and the activity of the compound 3 is equivalent to that of a positive control drug mitomycin. Particularly, the compound 3 also has good cytotoxicity to drug-resistant liver cancer cells (Huh7-LR cells), and the IC50 value of the compound 3 is 9.6 + / -1.1 micromole per liter. The compound 3 shows huge potential as a lead compound by virtue of the novel chemical structure and potent cytotoxicity shown in various cell lines.

The invention belongs to the technical field of PET, and discloses a flame-retardant high-strength PET composite material and a preparation process thereof. The composite material comprises 80-97 parts by weight of polyethylene terephthalate, 3-20 parts by weight of rosin-based phosphate polyester flame retardant, and 0.2-0.3 parts by weight of an antioxidant; the PET polyester is blended and modified by the rosin-based phosphate polyester flame retardant; the PET polyester contains an ethylene terephthalate structural unit and has a high structural similarity with the PET polyester, so that the polyester flame retardant can be uniformly dispersed in the PET polyester without affecting the mechanical properties of the material; and the composite material contains a condensed ring structure of rosin tricyclic diterpenes and a phosphate flame retardant group, so that the high temperature resistance and flame retardancy of the PET material are improved.

The application discloses a Daphnane type macrocyclic diterpenoid compound with a 4,7-oxygen bridge structure and a preparation method and application thereof. The monomer compound is obtained by repeatedly separating and purifying an ethyl acetate part extract of Daphne giraldii by using various separation fillers and separation technologies. The new compound is subjected to structural identification by using various 1D and 2D nuclear magnetic resonance spectrometry and high resolution mass spectrometry (HR-ESI-MS) and the like, and a molecular formula and a structural formula thereof are deduced. Further in-vitro cell activity researches show that the Daphnane type macrocyclic diterpenoid compound with the 4,7-oxygen bridge structure separated from the Daphne giraldii has good inhibitory activity on melanoma cells. The application is helpful to development and utilization of natural compounds of the Daphne giraldii.

The invention belongs to the field of marine natural products, and relates to indole diterpene alkaloid and application thereof as an antibacterial agent. The indole diterpenoid alkaloid compound 1 has a strong cell proliferation inhibition effect, and the IC50 value of the indole diterpenoid alkaloid compound 1 on a human gastric cancer cell strain SGC-7901 is 19.16 [mu] g / mL; and the IC50 value on a human hepatoma cell line HepG2 is 6.27 mu g / mL. The IC50 of the positive drug cis-platinum to SGC-7901 and the IC50 of the positive drug cis-platinum to HepG2 are 3.56 mu g / mL and 1.99 mu g / mL respectively. The structure of the compound 1 is shown in the specification.