Method used for extracting cembrenediols from tobacco inflorescence
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A technology for ceberane diterpenes and tobacco inflorescences is applied in the field of extracting biologically active substances, which can solve the problems of wasting tobacco leaf resources, requiring expensive instruments, complicated extraction steps, etc., and achieves reduction in the use of chemical pesticides, simple steps, and saving of solvent consumption. and the effect of chromatographic material consumption
Active Publication Date: 2015-10-28
TOBACCO RES INST CHIN AGRI SCI ACAD
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[0008] In order to overcome the problems of wasting tobacco leaf resources, cumbersome extraction steps, and needing expensive instruments in the current extraction process of cetrazane diterpene, the present invention uses waste tobacco inflorescences as the extraction material of cetrazane diterpene, and applies solvent precipitation technology at the same time Activated carbon was added to remove the higher alkanes and pigments in the extract respectively to obtain the crude extract of cetahane diterpene, which was used to inhibit the pathogen of apple rot
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Embodiment 1
[0039] 1. Sampling
[0040] Tobacco field flowering period, select 667m 2 (one mu) planted flue-cured tobacco varieties, tobacco inflorescences are obtained after topping, and fresh flue-cured tobacco inflorescences are used as extraction materials.
[0041] 2. Leaching
[0042] The fresh tobacco inflorescences were continuously leached twice in 3 glass beakers filled with 3L of dichloromethane, each time for 1s-2s. During the leaching process, when the color of the dichloromethane in the beakers turned yellowish brown, the solvent was replaced in time.
[0043] 3. Concentrate
[0044] The above extracts were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure at 40° C. to obtain 180 g of dark brown extract. The content of cembradin diterpene in the extract reached 60%, and the solvent was recovered at the same time.
[0045] 4. Precipitation of alkanes and removal of pigments
[0046] Take 5g of the extract, add 35mL of 70% eth...
Embodiment 2
[0056] Tobacco field flowering period, select 667m 2 (one mu) planted flue-cured tobacco varieties, collect tobacco inflorescences after topping, dry in the shade, use the dried flue-cured tobacco tobacco inflorescences as raw material, and all the other treatments are the same as in Example 1.
[0057] α-4,8,13-Cembratriene-1,3-diol and β-4,8,13-Cembratriene-1,3-diol have a purity higher than 95%, relative to flower surface secretion The recoveries of the extracts were 25.3% and 8.79%, respectively.
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Abstract
The invention relates to a method used for extracting cembrenediols from tobacco inflorescence. The method comprises following steps: 1, an organic solvent is adopted to extraction and condensation so as to obtain a tobacco flower surface glandular hair secreta crude extract; 2, the crude extract is subjected to solvent precipitation so as to remove higher alkanes, and pigments are removed with active carbon so as to obtain a tobacco diterpene extract product, wherein the tobacco diterpene extract product possesses broad-spectrum inhibition effect on plant pathogenic fungi, and especially excellent inhibition effect on apple rot; 3, the tobacco diterpene extract product is subjected to silica gel column chromatography successively so as to obtain two main diterpene compounds alpha-4,8,13-cembratriene-1,3-diol, and beta-4,8,13-cembratriene-1,3-diol with antitumor activity, neuroprotective agent activity, and bacteriostatic activity. Waste tobacco inflorescence is taken as the raw material, so that utilization of waste resources is realized; extraction step is simplified greatly, and cost is reduced; and product yield and purity are high.
Description
technical field [0001] The invention relates to a method for extracting biologically active substances from tobacco inflorescences, in particular to a method for extracting cembradane diterpenes from tobacco inflorescences. Background technique [0002] China is the world's largest tobacco leaf producing country, and its total tobacco leaf output accounts for about 41.5% of the world's total output. At present, my country's tobacco is mainly planted in impoverished mountainous areas such as Yunnan, Guizhou, and Sichuan. Tobacco planting has become an important way for farmers in impoverished areas to get rid of poverty. In recent years, driven by domestic and foreign tobacco control campaigns, the use of tobacco as a raw material for cigarettes has decreased significantly, posing challenges to rural economies in many tobacco-growing regions. In order to alleviate the dependence of tobacco cultivation on the demand for cigarette raw materials, scientists at home and abroad h...
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