32 results about "Biologically active substances" patented technology

The abstract relates to the field of pharmacy, in particular, to a method for obtaining extracts from medicinal plants and can be used for the production of biologically active additives, cosmetics and medicines with antioxidant, rejuvenating and anti-inflammatory effects. The essence of the utility model is the development of a technology for obtaining a liquid extract rich in biologically active compounds by ultrasonic processing of Rosa platyacantha Schrenk leaf raw materials. The method includes the following steps: 1. Preparation of plant raw materials (grinding to 5 mm); 2. Preparation of extractant (50% ethyl alcohol); 3. Extraction of raw materials and extractant in a ratio of 1:10 (Elma Elmasonic Easy 30H); 4. Filtration of the resulting liquid extract; 5. Packaging and labeling of the finished product. Extraction is carried out at a temperature of 35°C and a frequency of 37 kHz for 30 minutes. The technical result of the proposed approach is the development of a resource-saving technology for obtaining a liquid extract from the leaves of Rosa platyacantha Schrenk, ensuring a reduction in extraction time, a low temperature regime while preserving thermolabile biologically active substances, and an expansion of the raw material base through the use of previously unused parts of the plant.

Disclosed are container and cap assemblies for cryogenic storage of materials at temperatures below negative 150° C. The container and cap assemblies disclosed maintain container closure integrity and fluid-tightness at ambient and cryogenic temperatures, optionally to preserve biologically active substances stored therein.

It is an object of the present invention to provide a method for efficiently producing extracellular vesicles containing biologically active materials such as sonic hedgehog (Shh). The present invention provides, as a solution, a method for producing extracellular vesicles, comprising obtaining extracellular vesicles secreted from cells subjected to a treatment of inactivating Rab GTPase such as Rab18, wherein the cells are preferably cells expressing Shh. In addition, the present invention provides, as a solution, an extracellular vesicle containing Shh and having a particle diameter of 800 nm or more and / or a volume of 109 nm3 or more.

The invention relates to a novel and multifunctional instant curd formulation. This innovation achieves simultaneous lactose hydrolysis and protein coagulation within seconds of reconstitution. This innovation uniquely defers the acidification reaction until the point of use, producing a stable and shelf-friendly powder that instantaneously forms bioactive curd with therapeutic potential. The curd matrix generated serves as a smart carrier capable of delivering medicines, probiotic bacteria, or enzymes, which become activated and effective in situ due to the controlled biochemical environment created by the curd's specific pH. This invention represents a significant advancement over prior art by combining rapid curd formation with simultaneous generation of bioactive substances and enhanced delivery capabilities. It has broad applicability in food technology, pharmaceutical delivery, with particular utility in patient-friendly formulations suitable for diverse populations. The process and product offer improved convenience, efficacy, and multifunctionality without compromising stability or safety.

Provided herein is a particle made of a protein covalently bound to a prebiotic carbohydrate, thereby forming a conjugate, wherein at least two said conjugates are covalently crosslinked via their carbohydrate units (e.g. by a phospho-di-ester bond). The said particle may be used for selectively promoting probiotic bacteria growth in the colon and may be used to deliver additional probiotic growth factors, or other bioactives and drugs to the colon. Furthermore, provided herein are methods for preparing the particle, and for delivering a substance bound to, or entrapped within the particle, into the gastrointestinal tract of a subject in need thereof.

A high-antioxidant tomato flavoring condiment is made from tomato peel and pomace and tomato sauce as raw materials. The mixture undergoes ultrasonic pretreatment and enzymatic pretreatment, followed by a three-step fermentation process using brewer's yeast, lactic acid bacteria, and acetic acid bacteria. The ultrasonic pretreatment involves adding olive oil, glyceryl monostearate, and citric acid to the tomato peel and pomace juice, followed by ultrasonic treatment. This invention enhances the release of bioactive substances and the dissolution of nutrients through ultrasonic pretreatment and enzymatic hydrolysis, thereby significantly increasing the content of antioxidants in the tomato peel and pomace and improving the product's antioxidant capacity. Combined with a three-stage fermentation process, the product develops a unique flavor. The original condiment juice is amber in color, has a refreshing and tangy taste without astringency, and possesses strong antioxidant activity and tomato aroma. Furthermore, marine materials such as seaweed and sea grapes are added as raw materials to prepare a marine-based condiment. After fermentation, these release amino acids, anthocyanins, and other substances, effectively improving the product's nutritional value.

The present application relates to a kind of methods for preparing biological composite material using waste paper shell, comprising the following steps: S100.high purity cellulose intelligent extraction process: waste paper shell is identified and sorted by artificial intelligence system, and high purity cellulose is extracted therefrom;S200.nanometer treatment process: the extracted high purity cellulose is converted into nanocellulose suspension by mechanical nanometerization process or chemical nanometerization process;S300.composite modification process: nanocellulose is used as reinforcing framework, and is mixed with matrix material and biological active substance to form biological composite sol homogeneous;S400.molding solidification process: biological composite sol is carried out molding treatment, and biological medical composite material is prepared.The present application establishes complete high-value technology path from municipal waste to medical implant, realizes full-chain AI integration, and improves the mechanical properties of composite material.

An oral delivery system for at least one biologically active substance includes an orally insertable substrate device having surfaces. Microcapsules including the at least one biologically active substance adhere to the substrate device. The microcapsules define a core encapsulating the at least one biologically active substance. The microcapsules include multi-layer shells including a water-soluble adhesive layer and a pH-responsive layer and the microcapsules are adhered to the substrate surfaces for controlled release triggered by pH and fluid flow. In preferred embodiments the orally insertable device is a pacifier, straw or cutlery.

This invention discloses an apple pomace ESP-Zein-lutein composite nanoparticle delivery system and its preparation method, belonging to the field of functional food carrier and bioactive substance delivery technology. ESP is purified from apple pomace through hot water extraction, TCA deproteinization, and dialysis decolorization. Combined with antisolvent precipitation and electrostatic adsorption technology, composite nanoparticles with uniform particle size and high stability are prepared. The optimized ratio is Zein:ESP:lutein = 1:0.4:0.08. In this system, Zein and lutein form a hydrophobic core, and ESP forms a shell through hydrogen bonding and electrostatic interactions. The lutein encapsulation rate reaches 90.5%, significantly higher than the 54.7% of the single Zein system, making it suitable for targeted delivery of functional food additives, antioxidants, and eye health-related products.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The present invention provides a flow path device and an electroporation method that have high productivity. Provided is a flow path device that is used for electroporation and that, by applying an electric field via an electrode pair to a suspension which contains biological matter and a bioactive substance, introduces the bioactive substance into the biological matter. The flow path device comprises: a main flow path through which a suspension flows; a sheath liquid flow path through which flows only a sheath liquid that is a liquid other than the suspension and which causes the sheath liquid to flow into the main flow path; and an electrode pair which apply an electric field to the suspension flowing through the main flow path. The sheath liquid flow path has a sheath liquid expansion part that expands the flow path of the sheath liquid in the width direction, and a first flow path that is downstream of the sheath liquid expansion part and that is continuous with the sheath liquid expansion part. The total of the length of the first flow path and the length of the sheath liquid expansion part is not less than 0.8 times the width of the main flow path between the electrode pair.

Methods and systems and related particles and compositions that can be used to deliver a biologically active cargo such as a drug to a cornea of an individual, the delivery performed in a rapid, nonsurgical, and / or controllable fashion are described. Particularly, a method is described for controlled ballistic delivery of a cargo in a microparticle to the cornea of an individual, including a biologically active cargo.

The application relates to an ecological toilet with the functions of degrading excrement and concentrating urine and a method, and belongs to the technical field of sanitary facilities. The ecological toilet comprises a double-cavity toilet, an excrement cavity opening and closing mechanism, a chemical fertilizer spraying mechanism, a biological chemical fertilizer mechanism and a urine concentration mechanism; the double-cavity toilet is internally provided with an excrement cavity and a urine cavity; the biological chemical fertilizer mechanism is internally provided with a preliminary decomposition cavity and a deep decomposition cavity which are not communicated with each other, and the preliminary decomposition cavity and the deep decomposition cavity are communicated through a flow guide device; the urine concentration mechanism is used for receiving the urine discharged from the urine cavity and performing negative pressure heating and concentration, and finally discharging concentrated urine which retains organic urea and biological active substances. A method for degrading excrement and concentrating urine after defecation is realized based on the ecological toilet. The application solves the harmless treatment and resource utilization problems of the existing separate toilet, realizes excrement resource utilization and urine concentration value increment.

The application discloses a kind of sea cucumber peptide with antitumor activity and ginsenoside composition preparation method and its application, it is related to biological activity material complex technical field, composition includes directional enzymatic sea cucumber oligopeptide, biotransformation rare ginsenoside and tumor microenvironment response carrier;Sea cucumber oligopeptide is proportioned with Rg3, Rh2, Compound K, Rk1, Rg5 and the like saponin, and is loaded by hyaluronic acid / folic acid modified disulfide bond crosslinking chitosan-lipid carrier, is accelerated release under weak acid, high glutathione or hyaluronidase condition.This composition has good stability and delivery efficiency, and can be used for antitumor auxiliary preparation, functional food and combined drug research.

The invention belongs to the technical field of food science and colloidal chemistry, and relates to zein-OSA starch-sodium caseinate ternary composite Pickering particles as well as a preparation method and application of an emulsion of the zein-OSA starch-sodium caseinate ternary composite Pickering particles. The ternary composite particle is formed by self-assembly of zein, OSA starch and sodium caseinate, and has a high zeta potential absolute value and optimal interface adsorption performance. When the particles are used for stabilizing an oil-water emulsion, the interfacial tension can be remarkably reduced, the droplet distribution is improved, and the storage stability is enhanced. The prepared emulsion is suitable for a food emulsification system and a bioactive substance delivery system, and has the advantages of being natural, safe, high in stability and good in industrialization prospect.

The present application relates to the technical field of traditional Chinese medicine research, in particular to a chemical component analysis method of Rosa roxburghii tratt matcha tablets and a mechanism research method of the tablets in treating type 2 diabetes. The present application identifies active substances in the Rosa roxburghii tratt matcha tablets through liquid chromatography-mass spectrometry (LC-MS) analysis, and a total of 43 monomer substances are identified. The present application screens and obtains target points of the identified compounds through various databases, and obtains 21 core target points related to diabetes by combining network pharmacology methods, and determines 9 bioactive substances in the Rosa roxburghii tratt matcha tablets related to the core target points of diabetes, including catechin-(4α→8)-catechin, epicatechin-(4β→8)-catechin, and rose acid, etc., which provides a theoretical basis for the research and development of functional food for assisting in reducing blood sugar and provides a theoretical basis for deep processing products of Rosa roxburghii tratt.

This disclosure relates to compositions including formulated sucralfate or other aluminum-crosslinked sulfated agents and one or more biologically active substances to mucosal surfaces within the GI tract and methods for the manufacture and the use of these compositions.

The application relates to a method for recovering high-purity natural betaine from sugar beet molasses. The method is used for solving the problems of low betaine extraction purity, complex process and high cost in the prior art, and is characterized in that: a chromatographic column is built, a strong acid cation exchange resin column is sequentially eluted with 5% inorganic salt solution and deionized water; the diluted sugar beet molasses is injected into a ligand exchange chromatography column, eluted with deionized water, and the effluent part of the betaine is collected; the concentration of the betaine is concentrated to 100-500 mg / mL; semi-preparative high performance liquid chromatography purification is carried out, the betaine solution is collected; after concentration, freeze-drying is carried out, and the betaine crystal is obtained. The orthogonality between the two chromatographies is adopted, impurities which are difficult to purify are eliminated, and the chromatographic purity of the betaine is improved to 99.5%. The application is applied to the field of separation and purification of bioactive substances in sugar beet molasses.

The present invention relates to a polymeric nanoparticle for oral administration of physiologically active substances and a preparation method therefor and, more specifically, to a carrier or polymeric hydrogel for delivery of a physiologically active substance and a preparation method therefor, wherein the carrier or polymeric hydrogel comprises: polymeric micelles including a PEG-based block copolymer prepared by a reversible addition–fragmentation chain transfer (RAFT) radical polymerization method using a polymer that has a specific functional group at the chain end thereof and a boronic acid group introduced into the backbone thereof; and a physiologically active substance that can be encapsulated in the polymeric micelles, including a type 2 diabetes drug, insulin, an anti-obesity agent, a DPP-4 inhibitor, or an anticancer agent.

This invention discloses a purification method applicable to collagen, hyaluronidase, and coenzymes, belonging to the field of bioengineering technology. This invention provides a universal purification system of "directional pretreatment - gradient membrane separation - multi-mode chromatography - precise purification - low-temperature molding," integrating innovative technologies such as hypotonic-mild fragmentation coupling, composite membrane-chromatographic synergistic enhancement, in-situ online cleaning enhancement, and real-time monitoring and control of product stability, achieving efficient separation and purification of various bioactive substances. It solves the problems of traditional purification processes, such as strong targeting, poor versatility, significant product activity loss, unstable purity, and high industrialization costs. Product purity is ≥98%, activity retention rate is ≥97%, and media lifespan is extended by more than 3 times. It is suitable for high-end fields such as pharmaceuticals, cosmetics, and food, and has significant industrialization value.

The application provides a Hermetia illucens antibacterial peptide extraction method, and relates to the technical field of bioactive substance extraction and biological preparation, which comprises the following steps: adding brown sugar, Aspergillus oryzae and lactic acid bacteria into water, standing and fermenting at 30 DEG C for 24h-48h to prepare a fermentation induction solution, spraying and uniformly mixing 5th instar Hermetia illucens larvae with the fermentation induction solution according to a mass-volume ratio of 5g:1mL after the 5th instar Hermetia illucens larvae are starved for 6h, wherein the Hermetia illucens antibacterial peptide extraction method realizes efficient extraction and powder preparation of the antibacterial peptide by implementing fermentation induction on the 5th instar Hermetia illucens larvae and sequentially performing homogenization, complex enzymolysis, acidification and centrifugation, ultrafiltration concentration and spray drying treatment. The application systematically integrates the processes of induced generation, release separation and enrichment drying of the antibacterial peptide, which is beneficial to improving the extraction efficiency of the antibacterial peptide and the enrichment degree of active ingredients of the Hermetia illucens antibacterial peptide, and obtaining the antibacterial peptide powder product which is convenient to store, transport and apply.

This invention relates to the field of pharmaceutical formulation technology, specifically to a hydrophobically modified alginate oligosaccharide micelle, its preparation method, and its application. The alginate oligosaccharide micelle provided by this invention uses alginate oligosaccharide as a raw material, and is obtained by reacting alginate oligosaccharide with hydrophobic fatty acids. The micelles provided by this invention are nanoscale, and their structure of a hydrophobic core and hydrophilic shell can effectively load lipid-soluble substances. Simultaneously, by introducing hydrophobic fatty acids for derivatization modification, on the one hand, derivatization modification can structurally alter the amphiphilicity of alginate oligosaccharide, developing its function of encapsulating hydrophobic bioactive substances; on the other hand, derivatization modification of alginate oligosaccharide can endow the micelles with active targeting or responsive release capabilities, significantly improving the bioavailability of hydrophobic bioactive substances.

A substrate (12) for delivering a biologically active substance, the substrate (12) comprising: a cross-linked silicone matrix formed by cross-linking a silicone polymer using at least one crosslinking agent comprising a tetraalkoxysilane, the cross-linked silicone matrix defining a plurality of domains, and a composition contained within the domains of the cross-linked silicone matrix, wherein the composition comprises: at least one monomeric glycol and at least one biologically active substance.

This invention provides a lipid compound and its applications, the results of which are shown in Formula I. The lipid compound provided by this invention can be used for targeted delivery of bioactive molecules to splenic T cells, particularly suitable for transporting negatively charged nucleic acid molecules, such as mRNA, providing a potential delivery strategy for in vivo specific gene editing of T cells. This invention also provides a bioactive substance delivery system comprising the lipid compound, which is loaded with bioactive substances. The bioactive substance delivery system of this invention, after intravenous administration, can efficiently target spleen tissue and selectively deliver active substances to T cells within the spleen, achieving efficient T cell transfection and immune activation. The delivery system of this invention has a simple synthesis process and strong structural tunability, and has broad application prospects in the fields of T cell immunotherapy and gene therapy.

The present application relates to a kind of mannose modified macrophage targeted nanoparticles and its preparation method and application, and the nanoparticles include mannose-soy protein peptide copolymer and its loaded bioactive substance, wherein the mass ratio of soy protein peptide and mannose in the mannose-soy protein peptide copolymer is (1-3):(1-2), and the mass ratio of the mannose-soy protein peptide copolymer and bioactive substance is (20-60):1.Compared with the prior art, the bioactive substance-loaded nanoparticles of the present application are mannose-modified, the wall material used has good biocompatibility and high safety, the preparation method is simple, easy to control and operate, and there is no toxic organic solvent residue, which can significantly promote the phagocytosis of active substances by macrophages, improve the bioavailability of bioactive substances and promote the activity change of macrophages.

The present invention provides a flow channel device with high productivity. Provided is a flow channel device which is used for electroporation, and which introduces a biologically active substance into a biologically derived material by applying, by means of an electrode pair, an electric field to a suspension that contains the biologically derived material and the biologically active substance. The flow channel device comprises: a main flow channel through which the suspension flows; and the electrode pair that applies the electric field to the suspension flowing through the main flow channel. The main flow channel comprises: a suspension inflow port through which the suspension flows into the main flow channel; an expanded part in which the flow channel is expanded in the width direction with the suspension inflow port used as the starting point; and a main flow channel body part that is disposed in the downstream of the expanded part and is continuous with the expanded part. A side surface of the expanded part does not have a bent part.

This invention discloses a curcumin-loaded oleogel and its preparation method. The method includes: extracting nanocellulose from pineapple leaves, compounding it with chitosan, and freeze-drying to prepare a chitosan / nanocellulose composite aerogel; dissolving curcumin in edible oil to obtain a curcumin-containing oil phase solution; immersing the composite aerogel in the oil phase solution and shaking to adsorb excess oil from the surface, thus obtaining the curcumin-loaded oleogel. The composite aerogel prepared by this invention forms a dense network structure internally, exhibiting excellent oil absorption (16.20 g / g) and oil retention (68.57%). The obtained oleogel has a curcumin loading rate as high as 52.24%, can achieve sustained release in a simulated gastrointestinal environment, and has good antioxidant activity, with a DPPH free radical scavenging rate of 75.24% after 14 days of storage. This invention provides a new solution for functional oil substitutes and the delivery of bioactive substances.

The application discloses a preparation method of a three-acid chelating compound fertilizer, and belongs to the technical field of new-type fertilizers. The three acids include gamma-aminobutyric acid (GABA), polyglutamic acid (PGA) and fulvic acid. The application innovatively adopts a three-step process of "pre-chelation reaction-molecular embedding-low-temperature granulation", first forms pre-chelation complexes of the three acids under specific pH and temperature conditions, then protects the heat-sensitive three acids by a sodium alginate-calcium chloride embedding technology, and finally granulates under a temperature lower than 55 DEG C, thereby solving the technical problem that bioactive substances are prone to inactivation in traditional high-temperature processes. Through the intermolecular synergistic chelation and hierarchical release mechanism of the three acids, the application realizes the synergy of rapid stress resistance response and long-acting nutrient activation, and the phosphorus utilization rate is increased by more than 40%, and is suitable for use in the seedling stage, flowering stage and stress period of crops.