315 results about "Biologically active substances" patented technology

The invention discloses a near-infrared responsive injectable MXene / fibroin / carboxymethyl chitosan / oxidized hyaluronic acid nano composite hydrogel SCO (at) M, and relates to the technical field of biomedical materials. The method disclosed by the invention comprises the following steps: 1) preparing an MXene single-layer nanosheet by adopting LiF-HCl in-situ HF etching; (2) modifying the fibroin through glycidyl methacrylate to obtain SF-MA; 3) oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid OHA; (4) uniformly dispersing MXene in the SF-MA solution, and sequentially adding carboxymethyl chitosan CMCS and OHA, so as to prepare the injectable nano composite soft hydrogel with the shear thinning characteristic; and 5) through ultraviolet irradiation crosslinking, rapid in-situ curing of the hydrogel is realized, and the mechanical strength of the hydrogel is improved. The hydrogel provided by the invention has excellent injectability and mechanical property, has good photo-thermal antibacterial property, oxidation resistance and cell compatibility, can be used as a carrier of cells, medicines and bioactive substances in the field of biological medicines, and has a wide application prospect.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention relates to the technical field of polymer degradable materials, in particular to a biodegradable material as well as a preparation method and application thereof. The material takes modified polylactic acid as a main body, and an antioxidant, a plasticizer, a flame retardant and an inorganic filler are used as auxiliary materials in parts by weight. The method is characterized in that lactic acid and an epicatechin imidazole ammonium chloride polymer are polymerized under the catalysis of stannous octoate, and an epicatechin bioactive group is introduced into a polylactic acid main chain in a chemical bonding manner, so that the biodegradation rate, the mechanical property and the thermal stability of the material are remarkably improved. Bioactive substances and ionic groups in the material promote microbial degradation, and the material can be completely decomposed in a compost environment in a short time; meanwhile, the strength, the toughness and the high-temperature processability of the polylactic acid are effectively improved through molecular-level enhancement and an ionic cross-linking effect. The material can be widely applied to the fields of disposable tableware, food packaging films, degradable agricultural mulching films and the like.

The present invention belongs to the field of probiotic fermentation technology, and specifically relates to a fermented product of Bifidobacterium longum subsp. infantis, and a preparation method thereof, and applications thereof in improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue. The Bifidobacterium longum subsp. infantis NKU FB3-14 composition of the present invention and a probiotic fermented product prepared using the Bifidobacterium longum subsp. infantis NKU FB3-14 composition as a raw material have bioactive substances that are different from the raw materials, thereby achieving the effects of improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The present invention relates generally to compositions, formulations, and methods for immunotherapy and drug delivery, including the treatment of eye diseases such as dry eye disease (DED). In particular, the present invention relates to methods of producing exosomes from mesenchymal stem cells (MSC-Exos) and, optionally, loading said exosomes with one or more bioactive substances. The exosomes may be loaded using electroporation with one or more bioactive substances such as proteins, miRNAs, and / or siRNA. In specific embodiments, the exosomes, and / or one or more biological compounds derived from the exosomes, may be provided to an individual in need thereof, including as part of one or more compositions containing mesenchymal stem cells (MSCs). The individual in need thereof may be an individual having a medical disorder such as DED, immune disorders, cancer, and other disorders. Therefore, the compositions containing MSCs can be used for topical application to the eye. The compositions can contain MSCs, MSC-Exos, one or more MSC-derived biological compounds (e.g., growth factors, proteins, etc.).

A dry drug delivery system comprising a middle layer encased in two outer layers, the middle layer and the two outer layers each comprising a mixture of electrospun fibers. Also disclosed is a water-dissolvable dry device containing a physical mixture of a first fiber and a second fiber in which the first fiber and the second fiber are configured to react with each other and with a biological tissue upon hydration, and the device transforms into a hydrogel upon contact with the biological tissue. Further provided is a method for delivering a bioactive substance to a soft tissue using the system or device.

The invention discloses a preparation method of deer spleen aminopeptide powder in the field of bioactive substance extraction and functional material compositing, and the performance of the deer spleen aminopeptide powder is improved by compositing a multi-stage functional modified material and deer spleen aminopeptide. The preparation method comprises the following steps: cleaning, homogenizing and crushing fresh deer spleen, extracting with a phosphate buffer solution, adjusting the pH value, carrying out enzymolysis, inactivating enzyme, and treating with an ultrafiltration membrane and a nanofiltration membrane in a specific molecular weight range to obtain a concentrated solution; and mixing the concentrated solution with a multi-stage functional modified material under proper conditions, sterilizing, pre-freezing, and freeze-drying to obtain the deer spleen aminopeptide powder. Wherein the modified material is constructed through quaternization modification, nanopore loading, enzyme inhibitor coupling and targeting ligand modification, and has the functions of charge repulsion, steric hindrance, enzyme inhibition and targeting recognition. The deer spleen aminopeptide powder prepared by the invention has high stability and good bioavailability, and is suitable for functional food or health care products.

The invention discloses a microbial liquid inoculant containing streptomyces lydicus as well as a preparation method and application of the microbial liquid inoculant, belongs to the technical field of microorganisms, and provides the microbial liquid inoculant containing the streptomyces lydicus, and the microbial liquid inoculant containing the streptomyces lydicus is prepared from 40 to 50 parts of streptomyces lydicus fermentation liquor, 3 to 7 parts of bacillus amyloliquefaciens fermentation liquor, 0.1 to 0.6 part of polysaccharide and 0.1 to 0.5 part of a dispersing agent. According to the microbial liquid fungicide containing the streptomyces lydicus, the streptomyces lydicus has a plant protection function, the polysaccharide has a non-specific protection effect on various bioactive substances, and the bacillus amyloliquefaciens and the streptomyces lydicus have a synergistic effect, so that the effects of resisting diseases, promoting growth and improving the quality and yield of crops are achieved. The preparation method of the microbial liquid inoculant is simple in process, low in preparation cost, low in energy consumption and suitable for popularization and application, and extraction and drying processes are avoided.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The invention relates to a preparation method of aronia melanocarpa relaxing juice, and belongs to the field of food processing. The preparation method comprises the following steps: adding an edible gelatin aqueous solution with a freezing force value of 120-250Bloom g into aronia melanocarpa juice to obtain a mixed solution, adjusting the pH value of the mixed solution to 2-5, carrying out a water bath reaction, rapidly cooling, standing, filtering, sterilizing and canning to obtain the aronia melanocarpa softening juice. According to the method, specific precipitation of condensed tannin is realized by accurately regulating and controlling molecular conformation and reaction pH of gelatin, so that the content of condensed tannin in the aronia melanocarpa juice is reduced, adverse symptoms such as diarrhea and gas displacement increase caused by the condensed tannin are effectively relieved, meanwhile, a bioactive substance anthocyanin in the aronia melanocarpa juice is not destroyed by the method, and the method is suitable for large-scale production of the aronia melanocarpa juice. The raw material composition is simple, and the process is convenient and easy to implement.

The invention provides a preparation method and application of an intestinal adhesion delivery system based on pea 2S albumin. The intestinal adhesion delivery system is a protein-polyphenol compound generated by covalent binding of quinone obtained by oxidizing polyphenol through periodate resin and pea 2S albumin. According to the intestinal adhesion delivery system, cysteine-rich pea 2S albumin is used as a delivery carrier, polyphenol bioactive substances are loaded on the cysteine-rich pea 2S albumin, release of functional components in the digestion process is effectively delayed, and the gastrointestinal stability is improved. The composition is applied to the fields of inflammatory bowel diseases and intestinal infection, can target the diseased region of colitis, and effectively relieves the symptoms of colitis by enhancing the barrier function of intestinal mucosa, regulating intestinal flora and inhibiting inflammatory response. The intestinal adhesion delivery system can also deliver other bioactive substances, is applied to the fields of biological science, functional food and the like, and has good development potential.

The invention relates to the technical field of bioactive substance preparation, and provides a lentil low-molecular-weight glucan with immunocompetence and a preparation method of the lentil low-molecular-weight glucan with immunocompetence, and the lentil low-molecular-weight glucan with immunocompetence is obtained by separating and purifying lentil. The extraction method comprises the following steps: crushing dried lentils, extracting by adopting a compound enzyme-assisted extraction method, freeze-drying to obtain crude polysaccharide, dissolving the crude polysaccharide with distilled water, performing deproteinization treatment by adopting a sevag method, separating the deproteinized solution through an anion exchange cellulose column, collecting effluent, and treating to obtain freeze-dried powder, and dissolving the freeze-dried powder, purifying, carrying out color development tracking, collecting a polysaccharide solution of a color development positive part, and freeze-drying to obtain the purified polysaccharide. The invention solves the problems in the prior art that deep exploration on the structure-function relationship and immunocompetence of the polysaccharide is lacked, and the development of the polysaccharide as an immunomodulatory functional raw material is limited.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

A nanoparticle having a solid core comprising a biologically active substance, said core being enclosed by an inorganic coating, a method for preparing the nanoparticle, and the use of the nanoparticle in therapy. A kit comprising the nanoparticle and a pharmaceutical composition comprising the nanoparticle.

The invention discloses a nanofiber based on a polypeptide derivative and an enzymatic self-assembly method and application of the nanofiber based on the polypeptide derivative, the sequence of the polypeptide derivative is Nap-Gly-Phe-Phe-pTyr-Lys-Trp-Tyr-Gln-Asn-Met-Ile-Arg, and the Gly-Phe-Phe-pTyr is of an L configuration or a D configuration. The self-assembled polypeptide derivative disclosed by the invention contains an alkaline phosphatase restriction enzyme cutting site, and the self-assembled polypeptide derivative is self-assembled at a specific part with high enzyme specificity expression through enzyme catalysis to form nanofibers. The nanofiber hydrogel obtained through enzymatic treatment is carried out under physiological conditions, high temperature or ultraviolet irradiation is not needed, damage of high temperature to bioactive substances is avoided, the nanofiber hydrogel is more suitable for a temperature-sensitive in-vivo environment, toxic chemical cross-linking agents or photoinitiators do not need to be introduced, the cytotoxicity of the material is remarkably reduced, active enzymes or drugs can be embedded in situ, and the nanofiber hydrogel can be used for in-situ treatment. The biological function is maintained.

The present invention relates to a CD117 antibody and a delivery vector using same, such as lipid nanoparticles (LNPs). Further provided in the present invention are a method for delivering a bioactive substance to CD117+ cells by using the delivery vector and a method for preparing such a lipid nanoparticle conjugate.

The invention provides lotus seed wine and a preparation method thereof.The lotus seed wine is prepared through the steps of fermentation main material pretreatment, fermentation auxiliary material pretreatment, vertical imgabs0 # fermentation wine production, continuous imgabs1 # fermentation wine production, storage and blending. The lotus seed wine is high in wine yield and has high-content bioactive substances and flavor substances, particularly, the content of alkaloid in the lotus seed wine is obviously increased, the wine body is mellow, soft, sweet, full, fine, harmonious and refreshing, and the aftertaste of the lotus seed is long-lasting.

The invention belongs to the technical field of fermentation and metagen preparations, and particularly relates to application of bifidobacterium breve metagen in the aspects of scavenging free radicals and relieving oxidative aging. According to the present invention, the oxidative free radical scavenging experiment results show that the bifidobacterium breve NKU BB 1-2 metagen has good oxidative free radical scavenging ability, and has good scavenging ability on hydroxyl free radicals, superoxide anions and DPPH free radicals; meanwhile, through component analysis, it is found that the bifidobacterium breve NKU BB 1-2 metagen contains various antioxidant and anti-aging bioactive substances such as flavonoids, indoles, amino acids, bile acids and ceramides; a cell model experiment verifies that the bifidobacterium breve NKU BB 1-2 metagen can obviously relieve oxidative damage to the cells and improve the oxidation resistance of the cells, so that oxidative aging is relieved.

The invention discloses a Pickering emulsion for embedding beta-carotene as well as a preparation method and application of the Pickering emulsion. The Pickering emulsion is obtained by embedding beta-carotene in an emulsifying agent; the emulsifier is a suspension prepared according to the following method: S1, extracting freeze-dried water extraction residues of pterocarya palustris by using an alkaline solution to obtain a filtrate; s2, adjusting the pH value of the filtrate to 6.8-7.3, and centrifuging to obtain a precipitate; s3, dispersing the precipitate in a phosphate buffer solution, performing hydration and shearing treatment, and then performing ultrasonic treatment. The Pickering emulsion disclosed by the invention has a higher BC retention rate after being stored for a short time under low-temperature, dark and low-oxygen conditions; the Pickering emulsion disclosed by the invention still has very high biological accessibility after being subjected to simulated in-vitro digestion, so that the Pickering emulsion stabilized by suspension liquid has very large potential in the aspect of conveying bioactive substances, and theoretical support is provided for practical application of the Pickering emulsion.

The utility model relates to the technical field of medical instruments, and discloses a tissue repair spraying device which comprises a storage tank, the top of the storage tank is fixedly connected with a concave circular ring, the top of the concave circular ring is fixedly connected with a sleeve, one side of the sleeve is provided with a through hole, and the circumferential inner wall of the sleeve is rotationally sleeved with a rotating cylinder. A plurality of spraying holes with different sizes are formed in the circumferential surface of the rotating cylinder, a rotating rod is fixedly connected to the middle position of the top of the rotating cylinder, the top of the rotating rod penetrates through the sleeve to be fixedly connected with a rotating handle, the spraying holes with different sizes can reach the through hole through rotation of the rotating handle, and the spraying range is adjusted; a round hole matched with the rotating rod is formed in the middle of the top of the sleeve. According to the utility model, the technical effects that the spraying range can be quickly adjusted according to the area or shape of the damaged tissue of a patient, so that the bioactive substances at the damaged tissue are uniformly sprayed and are not easy to waste are achieved.

The abstract relates to the field of pharmacy, in particular, to a method for obtaining extracts from medicinal plants and can be used for the production of biologically active additives, cosmetics and medicines with antioxidant, rejuvenating and anti-inflammatory effects. The essence of the utility model is the development of a technology for obtaining a liquid extract rich in biologically active compounds by ultrasonic processing of Rosa platyacantha Schrenk leaf raw materials. The method includes the following steps: 1. Preparation of plant raw materials (grinding to 5 mm); 2. Preparation of extractant (50% ethyl alcohol); 3. Extraction of raw materials and extractant in a ratio of 1:10 (Elma Elmasonic Easy 30H); 4. Filtration of the resulting liquid extract; 5. Packaging and labeling of the finished product. Extraction is carried out at a temperature of 35°C and a frequency of 37 kHz for 30 minutes. The technical result of the proposed approach is the development of a resource-saving technology for obtaining a liquid extract from the leaves of Rosa platyacantha Schrenk, ensuring a reduction in extraction time, a low temperature regime while preserving thermolabile biologically active substances, and an expansion of the raw material base through the use of previously unused parts of the plant.

The present invention provides novel triazolopyrimidines derived from nucleobase thymine, and methods of producing the same, as well as various biologically active substances obtained by the methods, particularly antitumor agents. The triazolopyrimidine compounds represented by the following formulas (I) to (VIII) (wherein R represents a hydrogen atom, an alkyl group, or an aryl group) and methods of producing the same.

A method for producing particles is provided. The method includes: preparing a bioactive-substance-containing liquid that includes a bioactive substance and a solvent; discharging the bioactive-substance-containing liquid as droplets; and removing the solvent from the droplets to form particles, wherein a biological activity ratio represented by {(biological activity level B / biological activity level A)×100} is 80% or greater, where the biological activity level A is a biological activity level of the bioactive substance before the preparing the bioactive-substance-containing liquid, and the biological activity level B is a biological activity level of the bioactive substance after the preparing of the bioactive-substance-containing liquid.

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

Disclosed are container and cap assemblies for cryogenic storage of materials at temperatures below negative 150° C. The container and cap assemblies disclosed maintain container closure integrity and fluid-tightness at ambient and cryogenic temperatures, optionally to preserve biologically active substances stored therein.

the present invention provides a method for producing a protein having a supramolecular structure in which a bioactive substance is encapsulated, comprising: (I) bringing a subunit of a protein, which forms a supramolecular structure, a bioactive substance, and a solution for forming the protein having the supramolecular structure from the subunit into contact with one another in a flow micro mixer.

The present invention relates to a method for evaluating the binding affinity of a bioactive substance to cardiolipin based on the use of an acridinium salt as a fluorescent probe.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.

The present invention relates to a biologically active material conjugate in which a biological active material is conjugated with a biotin moiety, a fatty acid moiety, or a combination thereof and, more specifically, to a biologically active material conjugate having a biotin moiety and a fatty acid moiety coupled thereto. With a biotin moiety and a fatty acid moiety coupled thereto, the biologically active material according to the present invention exhibits an excellent in-vivo oral absorption rate and has an excellent pharmacokinetic effect.