548 results about "Biologically active substances" patented technology

There is provided a solid dosage form adapted for the release of a biologically active material in the oral cavity wherein the dosage form includes at least one biologically active material, and at least one matrix forming agent, wherein the dosage form substantially dissolves in the oral cavity. A method of producing the same and a kit including the same are also provided.

The invention provides a gamma-cyclodextrin-based metal organic framework composite material as well as preparation and application thereof. The metal organic framework composite material based on gamma-cyclodextrin comprises chitosan, gamma-CD-MOFs (Metal Organic Frameworks) and an active substance. The preparation method comprises the following steps: by taking chitosan as a film material base material, synthesizing gamma-CD-MOFs by utilizing a hydrothermal method, and then loading cinnamyl aldehyde with antibacterial property, 2-methoxycinnamyl aldehyde with ultraviolet light resistance and curcumin with an antioxidant effect into the gamma-CD-MOFs. And finally, mixing with chitosan with better film-forming property, and spraying on glass or the surfaces of fresh fruits. The gamma-CD-MOFs has the characteristic of slowly releasing bioactive substances, is non-toxic and is a material capable of being applied to food packaging.

The invention relates to a bacterial nanocellulose composite tube and a preparation method and application thereof, a three-dimensional network of bacterial nanocellulose is reserved, and the three-dimensional network is fully utilized as a dispersion phase to disperse introduced other macromolecules to form a homogeneous system; drugs such as heparin are encapsulated in situ or bioactive substances such as heparin are chemically grafted in the wall of the bacterial nanocellulose tube. Proteins such as silk fibroin, collagen, gelatin and fibronectin support adhesion and proliferation of cells, and the composite tube loaded with heparin has excellent anticoagulant activity and an effect of promoting endothelial cell function expression. The preparation process of the composite tube is simple, and the composite tube has a good application prospect in biomedical application of tubular artificial organs such as artificial blood vessels, artificial nerve conduits, artificial esophagus, artificial tracheas, bile ducts and the like.

The invention discloses lactococcus lactis subsp. Lactis and application thereof in fermentation of American cockroach powder. The strain is separated and screened from the intestinal contents of the Amolops nobilis, grows well on an MRS agar culture medium, is not hemolyzed, is sensitive to various common antibiotics, has relatively strong adaptive capacity to the intestinal environment of human and animals, has relatively strong lactic acid production capacity, and has strong inhibition capacity to common intestinal pathogens of human and animals. When the strain is used for fermenting American cockroach powder, the content of bioactive substances such as amino acids, peptides, indoles, pyrimidine and purines can be greatly increased, fermented sterile supernate has an obvious effect on mouse colitis induced by DSS, and the inflammatory factor level is remarkably reduced; the strain opens up a new way for further development and utilization of the traditional Chinese medicinal material periplaneta americana.

The invention provides a staged release type rigid-flexible composite nerve conduit as well as a preparation method and application thereof. A polymer fiber membrane with a double-layer nanofiber membrane structure is used as a tube wall, multi-section hydrogel comprising short-section hydrogel containing anti-inflammatory substances and long-section hydrogel containing growth factors is used as an inner core, and bioactive substance particles containing the growth factors are deposited on the inner wall of the polymer fiber membrane. The staged release type rigid-flexible composite nerve conduit is obtained. The composite nerve conduit provided by the invention is a rigid-flexible composite nerve conduit capable of controllably delivering anti-inflammatory substances and growth factors in time and space to meet the requirements of each stage of injury, and is combined with multiple induction signals such as a topological structure, chemotactic induction and the like to regulate and control the microenvironment of each stage after spinal cord injury and promote subsequent functional recovery.

The invention relates to the technical field of food processing, in particular to cubilose nourishing, conditioning and blood sugar reducing health-care food and a preparation process thereof. The cubilose enters the palace from the clear generation, becomes a defending tonic, is a medicinal and edible tonic, and has the beauty of a rare famous drug and an oriental treasure. The cubilose is rich in sialic acid, protein, amino acid and other bioactive substances, so that the cubilose has a nourishing effect and has certain health-care and conditioning effects on the lung, the kidney and the stomach. According to the preparation method, the cherry and the honeysuckle are subjected to synergistic fermentation through the rhamnolactobacilli and the saccharomycetes, so that the performance is improved, and the prepared cubilose health-care food has the effect of reducing blood sugar.

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention provides dual-response antibacterial repair-promoting injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. Two-dimensional titanium carbide (TiO2 (at) MXene) with the surface modified with titanium dioxide and a bioactive substance are introduced into a hydrogel matrix to serve as functional additives, TiO2 (at) MXene is a photo-thermal and ultrasonic response material, can emit heat under 808 nm near-infrared (NIR) light irradiation and low-intensity pulsed ultrasound (LIPUS) stimulation, and can generate reactive oxygen species (ROS) for sterilization under LIPUS stimulation; when the temperature of the hydrogel is increased, the bioactive substances can be slowly released from the hydrogel, and cell proliferation and tissue repair are promoted. The hydrogel provided by the invention can be coated on the surface of skin or injected under soft tissue, so that the functions of resisting bacteria in the early stage of inflammation and promoting tissue repair in the later stage are realized.

The invention discloses a near-infrared responsive injectable MXene / fibroin / carboxymethyl chitosan / oxidized hyaluronic acid nano composite hydrogel SCO (at) M, and relates to the technical field of biomedical materials. The method disclosed by the invention comprises the following steps: 1) preparing an MXene single-layer nanosheet by adopting LiF-HCl in-situ HF etching; (2) modifying the fibroin through glycidyl methacrylate to obtain SF-MA; 3) oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid OHA; (4) uniformly dispersing MXene in the SF-MA solution, and sequentially adding carboxymethyl chitosan CMCS and OHA, so as to prepare the injectable nano composite soft hydrogel with the shear thinning characteristic; and 5) through ultraviolet irradiation crosslinking, rapid in-situ curing of the hydrogel is realized, and the mechanical strength of the hydrogel is improved. The hydrogel provided by the invention has excellent injectability and mechanical property, has good photo-thermal antibacterial property, oxidation resistance and cell compatibility, can be used as a carrier of cells, medicines and bioactive substances in the field of biological medicines, and has a wide application prospect.

The invention provides a culture method for increasing the content of a bioactive substance Emodic acid in talaromyces purpurascens, and relates to the technical field of medicinal fungus culture. The culture method of the homopurple talaromyces spp. Comprises the following steps: carrying out liquid culture on hyphae of the homopurple talaromyces spp., and then inoculating the hyphae into a liquid culture medium containing black bean powder for culture. The defects in the prior art are overcome, and the pharmacological activity of the talaromyces purpurascens is enhanced by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, so that the content of Emodic acid in the talaromyces purpurascens is increased.

The invention provides a Wujin pig seminal plasma protein crude extract and application of the Wujin pig seminal plasma protein crude extract in an anti-freezing agent and an anti-freezing diluent for freeze preservation of Wujin pig seminal fluid. The Wujin pig seminal plasma protein crude extract is derived from Wujin pig seminal plasma, does not contain other exogenous bioactive substances, has a better freezing protection effect on sperms of Wujin pigs and is safer, and the acrosome integrity rate of frozen sperms of the Wujin pigs can be remarkably increased. According to the present invention, with the anti-freezing diluent, the vitality and the movement performance of the cryopreserved Wujin pig sperm can be significantly improved, the active oxygen level is reduced, the mitochondrial membrane potential and the energy metabolism are improved, and the oxidation resistance is enhanced, such that the vitality of the Wujin pig sperm can be easily improved, and the mitochondrial function can be easily maintained; the research provides key technical support for establishment of a Wujin pig semen cryopreservation technology, and has great practical significance for Wujin pig breeding and population propagation.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention belongs to the field of cell biology, particularly relates to stem cell exosome repair gel and a preparation method thereof, and more particularly relates to stem cell exosome repair gel containing active biological peptide and a preparation method thereof. The invention provides a repairing composition which comprises an active peptide as shown in SEQ ID NO.1 and a stem cell exosome. The repair gel provided by the invention contains the active peptide and the stem cell exosome loaded with the bioactive substances, and the components have a synergistic effect, so that the expression level of related cell factors can be improved, the repair after skin injury is promoted, and the physiological state of the skin is improved.

The invention discloses an anti-neurodegeneration exosome composition as well as a preparation method and application thereof. The anti-neurodegeneration exosome composition is prepared from the following components in parts by mass: 5 to 25 parts of human exfoliated deciduous tooth stem cell exosome, 25 to 85 parts of plant-derived exosome-like nano-vesicles, 12 to 10 parts of ginsenoside Rg, 2 to 10 parts of curcumin and 10 to 20 parts of auxiliary materials. The anti-neurodegeneration exosome composition contains a large amount of cell factors, growth factors, miRNA, mRNAs and other components, is easy to fuse with cell membranes of intestinal epithelial cells, can selectively deliver bioactive substances to recipient cells through a blood-brain barrier, carries out information transmission among different cells, regulates signal transduction among the cells, and has a good anti-neurodegeneration effect. The effects of resisting oxidation, resisting inflammation, resisting aging and the like are achieved.

The invention relates to the technical field of polymer degradable materials, in particular to a biodegradable material as well as a preparation method and application thereof. The material takes modified polylactic acid as a main body, and an antioxidant, a plasticizer, a flame retardant and an inorganic filler are used as auxiliary materials in parts by weight. The method is characterized in that lactic acid and an epicatechin imidazole ammonium chloride polymer are polymerized under the catalysis of stannous octoate, and an epicatechin bioactive group is introduced into a polylactic acid main chain in a chemical bonding manner, so that the biodegradation rate, the mechanical property and the thermal stability of the material are remarkably improved. Bioactive substances and ionic groups in the material promote microbial degradation, and the material can be completely decomposed in a compost environment in a short time; meanwhile, the strength, the toughness and the high-temperature processability of the polylactic acid are effectively improved through molecular-level enhancement and an ionic cross-linking effect. The material can be widely applied to the fields of disposable tableware, food packaging films, degradable agricultural mulching films and the like.

The present invention belongs to the field of probiotic fermentation technology, and specifically relates to a fermented product of Bifidobacterium longum subsp. infantis, and a preparation method thereof, and applications thereof in improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue. The Bifidobacterium longum subsp. infantis NKU FB3-14 composition of the present invention and a probiotic fermented product prepared using the Bifidobacterium longum subsp. infantis NKU FB3-14 composition as a raw material have bioactive substances that are different from the raw materials, thereby achieving the effects of improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue.

A device for transdermal electrochemical measurements, comprising a polymeric support having a surface provided for contact with skin, an array of polymeric microneedles integral with the support and projecting outward from the surface of the support, and a porous and / or nano / micro-structured bioelectrode. A biologically active species is immobilized on the surface of a conductive material. The microneedles and a surface of contact of the support with the skin are formed of a biocompatible crosslinked hydrogel, non-electron-conductive in the dry state and electrolyte-conductive upon contact with an aqueous fluid. The bioelectrode is arranged in contact with the hydrogel and is free of direct contact with the skin. Methods for preparing and using the device are also described.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention provides a composite nanoparticle with active substance sequential release capability, a multifunctional microneedle patch based on curcumin-loaded ZIF-67 nanoparticles, preparation methods of the composite nanoparticle and the multifunctional microneedle patch and application of the multifunctional microneedle patch, and belongs to the technical field of biology. The composite nano-particles with the active substance sequential release capability are CurZIF-67NPs, the average particle size is 403 + / -7 nm, the microstructure is dodecahedral structure particles with rough textures on the surface, and the composite nano-particles have the effects of slowly releasing bioactive substances, improving the inflammatory microenvironment and regulating and controlling osteogenesis under certain conditions. The composite nano-particle comprises curcumin powder and a ZIF-67 nano-material, the ZIF-67 nano-material can be decomposed to quickly release Co < 2 + >, macrophages are regulated and controlled to be polarized to an M2 type, and excessive immune inflammation reaction is improved; the curcumin powder is slowly released, so that the periodontal ligament stem cells can be regulated and controlled to be differentiated into osteoblasts, and the osteogenic activity is enhanced.

The present invention relates generally to compositions, formulations, and methods for immunotherapy and drug delivery, including the treatment of eye diseases such as dry eye disease (DED). In particular, the present invention relates to methods of producing exosomes from mesenchymal stem cells (MSC-Exos) and, optionally, loading said exosomes with one or more bioactive substances. The exosomes may be loaded using electroporation with one or more bioactive substances such as proteins, miRNAs, and / or siRNA. In specific embodiments, the exosomes, and / or one or more biological compounds derived from the exosomes, may be provided to an individual in need thereof, including as part of one or more compositions containing mesenchymal stem cells (MSCs). The individual in need thereof may be an individual having a medical disorder such as DED, immune disorders, cancer, and other disorders. Therefore, the compositions containing MSCs can be used for topical application to the eye. The compositions can contain MSCs, MSC-Exos, one or more MSC-derived biological compounds (e.g., growth factors, proteins, etc.).

The invention provides a degradable vaginal repair injection material and application thereof, and relates to the technical field of biomedical materials. The degradable vaginal repair injection material is formed by compounding a dispersing agent and ECM particles, the mass percent of the ECM particles is 20%-80%, and the particle size of the ECM particles is 100-1000 microns. According to the application, the ECM particles are used as a core repairing component, and the ECM particles are rich in bioactive substances such as growth factors and collagen, so that repairing and regeneration of vaginal tissues can be effectively promoted. After being injected into the vagina, the degradable vaginal repair injection material can fill relaxed sites to increase stress after injection, and stimulate tissue collagen regeneration along with the degradation of ECM particles to repair the mechanical properties of the vagina.

A dry drug delivery system comprising a middle layer encased in two outer layers, the middle layer and the two outer layers each comprising a mixture of electrospun fibers. Also disclosed is a water-dissolvable dry device containing a physical mixture of a first fiber and a second fiber in which the first fiber and the second fiber are configured to react with each other and with a biological tissue upon hydration, and the device transforms into a hydrogel upon contact with the biological tissue. Further provided is a method for delivering a bioactive substance to a soft tissue using the system or device.

The invention discloses a preparation method of deer spleen aminopeptide powder in the field of bioactive substance extraction and functional material compositing, and the performance of the deer spleen aminopeptide powder is improved by compositing a multi-stage functional modified material and deer spleen aminopeptide. The preparation method comprises the following steps: cleaning, homogenizing and crushing fresh deer spleen, extracting with a phosphate buffer solution, adjusting the pH value, carrying out enzymolysis, inactivating enzyme, and treating with an ultrafiltration membrane and a nanofiltration membrane in a specific molecular weight range to obtain a concentrated solution; and mixing the concentrated solution with a multi-stage functional modified material under proper conditions, sterilizing, pre-freezing, and freeze-drying to obtain the deer spleen aminopeptide powder. Wherein the modified material is constructed through quaternization modification, nanopore loading, enzyme inhibitor coupling and targeting ligand modification, and has the functions of charge repulsion, steric hindrance, enzyme inhibition and targeting recognition. The deer spleen aminopeptide powder prepared by the invention has high stability and good bioavailability, and is suitable for functional food or health care products.

The invention provides a poultry probiotic preparation prepared by fermenting selenium-resistant duck-origin lactobacillus plantarum. The poultry probiotic preparation is prepared by inoculating duck-origin lactobacillus plantarum with high selenium ion loading capacity into a culture medium and fermenting, the duck-origin lactobacillus plantarum with high load of selenium ions has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30182. The high-load selenium duck-origin lactobacillus plantarum obtained by domesticating and screening by adopting a method for gradually increasing the concentration of selenium ions in a culture medium contains small peptides, small molecular organic acids, digestive enzymes and the like. As the antibacterial peptide is rich in targeted antibacterial peptide, the antibacterial peptide has an obvious inhibition effect on harmful bacteria such as escherichia coli, salmonella, riemerella anatipestifer and the like. Other bioactive substances such as digestive enzyme contained in the feed can promote the growth and development of poultry and improve the product quality.

The invention provides a method for controlling batch-to-batch difference and improving specificity by changing raw material adding time after microsphere activation, and belongs to the technical field of biomedicine. The bioactive substances are added for coupling after the microspheres are activated for a certain time. According to the method, extra reagents or steps do not need to be introduced, batch-to-batch difference reduction and false positive reduction can be achieved only by optimizing the coupling object adding time, operation is easy, control is easy, reduction of false positive results caused by non-specific binding is facilitated, biological coupling reaction is more sensitive and reliable, and the method is suitable for industrial production. Therefore, the detection sensitivity, specificity and accuracy can be improved, and the kit has extremely high practical value in the fields which need large-scale production and have high requirements on product quality. The method provided by the invention can provide a more reliable and stable tool for biomedical research and clinical diagnosis, and has a good application prospect.

The invention discloses a microbial liquid inoculant containing streptomyces lydicus as well as a preparation method and application of the microbial liquid inoculant, belongs to the technical field of microorganisms, and provides the microbial liquid inoculant containing the streptomyces lydicus, and the microbial liquid inoculant containing the streptomyces lydicus is prepared from 40 to 50 parts of streptomyces lydicus fermentation liquor, 3 to 7 parts of bacillus amyloliquefaciens fermentation liquor, 0.1 to 0.6 part of polysaccharide and 0.1 to 0.5 part of a dispersing agent. According to the microbial liquid fungicide containing the streptomyces lydicus, the streptomyces lydicus has a plant protection function, the polysaccharide has a non-specific protection effect on various bioactive substances, and the bacillus amyloliquefaciens and the streptomyces lydicus have a synergistic effect, so that the effects of resisting diseases, promoting growth and improving the quality and yield of crops are achieved. The preparation method of the microbial liquid inoculant is simple in process, low in preparation cost, low in energy consumption and suitable for popularization and application, and extraction and drying processes are avoided.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The utility model relates to the technical field of extraction equipment, and discloses equipment for extracting bioactive substance polypeptide from animals and plants, which comprises a base, a high-frequency alternating electromagnetic field generator and a condenser, a barrel is fixedly connected to the top surface of the base, a barrel cover is detachably arranged on the top surface of the barrel, and a heating plate is arranged in the base; the high-frequency alternating electromagnetic field generator is fixed with the cylinder body, and the working end of the high-frequency alternating electromagnetic field generator corresponds to the cylinder body; the condenser is arranged on one side of the barrel. In the application, the arranged high-frequency alternating electromagnetic field generator can generate a high-frequency alternating electromagnetic field, animal and plant raw materials under the action of the high-frequency alternating electromagnetic field can be decomposed into very small bioactive peptides, and the bioactive peptides and water vapor generated by the animal and plant raw materials are dissociated together; and the bioactive peptide which is free together with the water vapor can be condensed into liquid through the condenser, so that the extraction of the bioactive substance polypeptide is completed.

The invention relates to the technical field of extraction and processing of radix puerariae and perilla frutescens, and particularly discloses a compound extract of radix puerariae and perilla frutescens as well as a preparation method and application of the compound extract. The preparation method comprises the following steps: slicing radix puerariae, cleaning with purple perilla, drying, adding water to adjust the water content, adding a leavening agent, and fermenting to a specific pH value to obtain a fermented product; and performing reflux extraction on the fermentation product by using a deep-eutectic solvent to obtain the deep-eutectic solvent extract. Mixing the extract with ethanol, and performing supercritical carbon dioxide extraction to obtain a supercritical carbon dioxide extract. Carrying out water extraction and alcohol precipitation treatment on the extract to obtain a primarily purified extract; and finally, separating by using macroporous resin to obtain the final compound extract of the radix puerariae and the perilla frutescens. According to the method, the extraction rate of flavonoid compounds and other bioactive substances in the compound extract is remarkably increased, so that the compound extract shows excellent effects in the aspects of reducing blood pressure, reducing blood sugar, reducing blood fat and the like, can be used as a daily diet supplement, and is widely applied to the fields of foods, medicines and health-care products.

The invention relates to a preparation method of aronia melanocarpa relaxing juice, and belongs to the field of food processing. The preparation method comprises the following steps: adding an edible gelatin aqueous solution with a freezing force value of 120-250Bloom g into aronia melanocarpa juice to obtain a mixed solution, adjusting the pH value of the mixed solution to 2-5, carrying out a water bath reaction, rapidly cooling, standing, filtering, sterilizing and canning to obtain the aronia melanocarpa softening juice. According to the method, specific precipitation of condensed tannin is realized by accurately regulating and controlling molecular conformation and reaction pH of gelatin, so that the content of condensed tannin in the aronia melanocarpa juice is reduced, adverse symptoms such as diarrhea and gas displacement increase caused by the condensed tannin are effectively relieved, meanwhile, a bioactive substance anthocyanin in the aronia melanocarpa juice is not destroyed by the method, and the method is suitable for large-scale production of the aronia melanocarpa juice. The raw material composition is simple, and the process is convenient and easy to implement.