225 results about "Biologically active substances" patented technology

The invention discloses a preparation method of deer spleen aminopeptide powder in the field of bioactive substance extraction and functional material compositing, and the performance of the deer spleen aminopeptide powder is improved by compositing a multi-stage functional modified material and deer spleen aminopeptide. The preparation method comprises the following steps: cleaning, homogenizing and crushing fresh deer spleen, extracting with a phosphate buffer solution, adjusting the pH value, carrying out enzymolysis, inactivating enzyme, and treating with an ultrafiltration membrane and a nanofiltration membrane in a specific molecular weight range to obtain a concentrated solution; and mixing the concentrated solution with a multi-stage functional modified material under proper conditions, sterilizing, pre-freezing, and freeze-drying to obtain the deer spleen aminopeptide powder. Wherein the modified material is constructed through quaternization modification, nanopore loading, enzyme inhibitor coupling and targeting ligand modification, and has the functions of charge repulsion, steric hindrance, enzyme inhibition and targeting recognition. The deer spleen aminopeptide powder prepared by the invention has high stability and good bioavailability, and is suitable for functional food or health care products.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The invention relates to the technical field of bioactive substance preparation, and provides a lentil low-molecular-weight glucan with immunocompetence and a preparation method of the lentil low-molecular-weight glucan with immunocompetence, and the lentil low-molecular-weight glucan with immunocompetence is obtained by separating and purifying lentil. The extraction method comprises the following steps: crushing dried lentils, extracting by adopting a compound enzyme-assisted extraction method, freeze-drying to obtain crude polysaccharide, dissolving the crude polysaccharide with distilled water, performing deproteinization treatment by adopting a sevag method, separating the deproteinized solution through an anion exchange cellulose column, collecting effluent, and treating to obtain freeze-dried powder, and dissolving the freeze-dried powder, purifying, carrying out color development tracking, collecting a polysaccharide solution of a color development positive part, and freeze-drying to obtain the purified polysaccharide. The invention solves the problems in the prior art that deep exploration on the structure-function relationship and immunocompetence of the polysaccharide is lacked, and the development of the polysaccharide as an immunomodulatory functional raw material is limited.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

A nanoparticle having a solid core comprising a biologically active substance, said core being enclosed by an inorganic coating, a method for preparing the nanoparticle, and the use of the nanoparticle in therapy. A kit comprising the nanoparticle and a pharmaceutical composition comprising the nanoparticle.

The present invention relates to a CD117 antibody and a delivery vector using same, such as lipid nanoparticles (LNPs). Further provided in the present invention are a method for delivering a bioactive substance to CD117+ cells by using the delivery vector and a method for preparing such a lipid nanoparticle conjugate.

The invention discloses a Pickering emulsion for embedding beta-carotene as well as a preparation method and application of the Pickering emulsion. The Pickering emulsion is obtained by embedding beta-carotene in an emulsifying agent; the emulsifier is a suspension prepared according to the following method: S1, extracting freeze-dried water extraction residues of pterocarya palustris by using an alkaline solution to obtain a filtrate; s2, adjusting the pH value of the filtrate to 6.8-7.3, and centrifuging to obtain a precipitate; s3, dispersing the precipitate in a phosphate buffer solution, performing hydration and shearing treatment, and then performing ultrasonic treatment. The Pickering emulsion disclosed by the invention has a higher BC retention rate after being stored for a short time under low-temperature, dark and low-oxygen conditions; the Pickering emulsion disclosed by the invention still has very high biological accessibility after being subjected to simulated in-vitro digestion, so that the Pickering emulsion stabilized by suspension liquid has very large potential in the aspect of conveying bioactive substances, and theoretical support is provided for practical application of the Pickering emulsion.

The abstract relates to the field of pharmacy, in particular, to a method for obtaining extracts from medicinal plants and can be used for the production of biologically active additives, cosmetics and medicines with antioxidant, rejuvenating and anti-inflammatory effects. The essence of the utility model is the development of a technology for obtaining a liquid extract rich in biologically active compounds by ultrasonic processing of Rosa platyacantha Schrenk leaf raw materials. The method includes the following steps: 1. Preparation of plant raw materials (grinding to 5 mm); 2. Preparation of extractant (50% ethyl alcohol); 3. Extraction of raw materials and extractant in a ratio of 1:10 (Elma Elmasonic Easy 30H); 4. Filtration of the resulting liquid extract; 5. Packaging and labeling of the finished product. Extraction is carried out at a temperature of 35°C and a frequency of 37 kHz for 30 minutes. The technical result of the proposed approach is the development of a resource-saving technology for obtaining a liquid extract from the leaves of Rosa platyacantha Schrenk, ensuring a reduction in extraction time, a low temperature regime while preserving thermolabile biologically active substances, and an expansion of the raw material base through the use of previously unused parts of the plant.

A method for producing particles is provided. The method includes: preparing a bioactive-substance-containing liquid that includes a bioactive substance and a solvent; discharging the bioactive-substance-containing liquid as droplets; and removing the solvent from the droplets to form particles, wherein a biological activity ratio represented by {(biological activity level B / biological activity level A)×100} is 80% or greater, where the biological activity level A is a biological activity level of the bioactive substance before the preparing the bioactive-substance-containing liquid, and the biological activity level B is a biological activity level of the bioactive substance after the preparing of the bioactive-substance-containing liquid.

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

Disclosed are container and cap assemblies for cryogenic storage of materials at temperatures below negative 150° C. The container and cap assemblies disclosed maintain container closure integrity and fluid-tightness at ambient and cryogenic temperatures, optionally to preserve biologically active substances stored therein.

the present invention provides a method for producing a protein having a supramolecular structure in which a bioactive substance is encapsulated, comprising: (I) bringing a subunit of a protein, which forms a supramolecular structure, a bioactive substance, and a solution for forming the protein having the supramolecular structure from the subunit into contact with one another in a flow micro mixer.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.

The present invention relates to a biologically active material conjugate in which a biological active material is conjugated with a biotin moiety, a fatty acid moiety, or a combination thereof and, more specifically, to a biologically active material conjugate having a biotin moiety and a fatty acid moiety coupled thereto. With a biotin moiety and a fatty acid moiety coupled thereto, the biologically active material according to the present invention exhibits an excellent in-vivo oral absorption rate and has an excellent pharmacokinetic effect.

The invention discloses a Pickering emulsion stabilized by full-component egg white protein-EGCG (epigallocatechin gallate) as well as a preparation method and application of the Pickering emulsion, and belongs to the field of functional carrier emulsion preparation. The full-component egg white protein-EGCG composite stabilizer is formed by compounding a cooked egg white protein microparticle solution and an EGCG solution through pH regulation and control, the full-component egg white protein-EGCG composite stabilizer can be used for well stabilizing a Pickering emulsion and loading bioactive substances; the prepared Pickering emulsion with the stable astaxanthin-loaded full-component egg white protein-EGCG compound has relatively strong self-supporting property, relatively high encapsulation efficiency, excellent rheological property, moisture distribution property and biologically active substance slow release property, can be used as a 3D printing material, and has good 3D printing stability and accuracy.

The invention belongs to the technical field of biological medicines, and particularly relates to a dual-targeting broad-spectrum chimeric bacteriophage antibacterial pharmaceutical preparation as well as a preparation method and application thereof. The antibacterial drug preparation comprises a bioactive substance and a liposome for coating the bioactive substance, the bioactive substance is a targeting chimeric M13 bacteriophage coupled with an antibacterial substance, and the surface of the liposome is coupled with a targeting antibody. According to the invention, the liposome and bacteriophage dual-targeting design is adopted, specific targeting to bacterial focuses can be realized, and the antibacterial substance is loaded on the mild M13 bacteriophage, so that the dosage of the antibacterial substance is reduced, and meanwhile, uncontrollable release of endotoxin is avoided; and finally, the intracellular and extracellular bacteria at the in-vivo focus are thoroughly killed under the cooperation of the liposome with the targeting capability and the customizable chimeric bacteriophage.

The method of obtaining modified carbon quantum dots by N-substitution of compounds from the coumarin group, consists, according to the invention, in the fact that the carbon quantum dots, obtained by carbonisation of biomass constituting, inter alia, 1 biologically active substance having free carboxyl groups, are modified by the formation of an amide bond in an aqueous medium, under normal conditions, and then purified using dialysis membranes. During grafting, the ratio of dye to EDC is 1 to 1.1. The invention also relates to the carbon quantum dots with a fluorescence emission spectrum in the range 380- 800 nm, obtained by this method.

Disclosed herein are compositions for use in a variety of agricultural methods, the compositions comprising a plurality of naturally derived lipid particles comprising a phospholipid, a non-polar lipid, and a surface modifier, and further comprising a biologically active substance.

Provided in the present invention are a steroid-cationic lipid compound having a structure as shown in formula (I) and the use thereof. The compound can be used in the preparation of a lipid nanoparticle (LNP) for delivering a therapeutic agent and / or a preventive agent. The LNP prepared using the steroid-cationic lipid compound of the present invention has relatively good stability and transfection efficiency. The use of the LNP for delivering a nucleic acid, e.g., an mRNA, can realize efficient and stable delivery of biologically active substances to target cells or organs, and can induce a high level of specific antibody responses and cellular immune responses in experimental animals. Moreover, the compound has better safety.

The application discloses starch-based colon double-targeting microgels, a preparation method and application thereof. The preparation method comprises the following steps: covalently connecting chondroitin sulfate to beta-cyclodextrin by EDC to prepare beta-cyclodextrin-chondroitin sulfate; encapsulating a hydrophobic bioactive substance in the hydrophobic cavity of the beta-cyclodextrin in the beta-cyclodextrin-chondroitin sulfate to prepare beta-cyclodextrin-chondroitin sulfate loaded with the bioactive substance; modifying dextrin by octenyl succinic anhydride to prepare modified dextrin; and making a first mixed reaction system comprising the beta-cyclodextrin-chondroitin sulfate loaded with the bioactive substance, the modified dextrin and sodium trimetaphosphate to have a crosslinking reaction to prepare starch-based colon double-targeting microgels. The starch-based colon double-targeting microgels have good encapsulation and protection effects on the bioactive substance, and have a targeting effect in the colon, so that the bioactive substance can be more effectively delivered.

The invention discloses a centella asiatica fermentation product and application thereof. According to the method, specific strains are adopted, and the probiotic fermentation process of centella asiatica is optimized, so that the content of bioactive substances in fermented centella asiatica is greatly increased, and the bioavailability and functional characteristics of the centella asiatica are remarkably improved. The centella asiatica fermentation product disclosed by the invention has excellent effects on inhibiting bacteria, resisting aging, relieving, moisturizing, scavenging free radicals, resisting oxidation and resisting inflammation, and is expected to be widely applied to cosmetics, daily necessities, food, health care products and medicines.

This invention discloses a method for constructing a three-dimensional in vitro blood-brain barrier model with barrier function and its application, belonging to the biomedical field. The method includes: preparing a light-controlled biofunctionalized hydrogel with methacrylamide gelatin as the main framework; preparing a suspension of neurovascular matrix components using astrocytes and neurons; uniformly mixing the light-controlled biofunctionalized hydrogel and the neurovascular matrix component suspension to obtain a homogeneous cell-hydrogel composite solution, and preparing it into three-dimensional hydrogel microspheres; transferring the three-dimensional hydrogel microspheres to the bottom of a Transwell culture dish insert to form a stable neurolateral matrix layer; and adding a suspension of brain microvascular endothelial cells into the lower chamber of the Transwell culture dish. This invention achieves the synergistic effect of multiple bioactive substances, overcoming the shortcomings of the single bioactive substance loading method in the prior art.

It is an object of the present invention to provide a method for efficiently producing extracellular vesicles containing biologically active materials such as sonic hedgehog (Shh). The present invention provides, as a solution, a method for producing extracellular vesicles, comprising obtaining extracellular vesicles secreted from cells subjected to a treatment of inactivating Rab GTPase such as Rab18, wherein the cells are preferably cells expressing Shh. In addition, the present invention provides, as a solution, an extracellular vesicle containing Shh and having a particle diameter of 800 nm or more and / or a volume of 109 nm3 or more.

A PLG copolymer material, called a PLG(p) polymer material adapted for use in a controlled release formulation for a bioactive material is provided, wherein the formulation exhibits a reduced initial burst effect when introduced into the tissue of a patient in need thereof. A method of preparing the PLG copolymer material is also provided, as are methods of use.

The present invention relates to an oral pharmaceutical composition comprising (i) a biologically active material conjugate in which a biological active material is conjugated with a biotin moiety, a

The invention relates to the technical field of nano microspheres, in particular to a preparation method and application of salicylic acid nano microspheres. Through the synergistic effect of a series of key technical links such as raw material selection, step-by-step crosslinking, particle size control and fine purification, the salicylic acid nano microspheres are prepared; salicylic acid is prepared into nano microspheres with low irritation, high stability, good solubility, high drug loading capacity, high encapsulation efficiency and controllable drug release behavior, controlled release and slow release of drugs are achieved, the problem that traditional salicylic acid is uneven in in-vitro release rate is solved, the controlled release characteristic compared with an original drug is shown after encapsulation, and the drug release behavior is controllable. Especially in an acidic environment, the microspheres can delay and continuously release drugs, and have intelligent responsiveness. The salicylic acid derivative-based nano-microspheres can simultaneously deliver various drugs or bioactive substances, so that synergistic treatment is realized, and the salicylic acid derivative-based nano-microspheres can be widely applied to the fields of cosmetics and medicines.

The invention relates to the field of medical beauty devices, in particular to a needle-length-adjustable vacuum pump type seal microneedle which is characterized in that a vacuum pump assembly is arranged, bioactive liquid such as essence is stored, the liquid is directly conveyed to a needle wire through the negative pressure effect, and when the needle wire punctures the skin, the liquid can be directly guided into the subcutaneous part along the needle wire; and the trouble of step-by-step operation of firstly smearing liquid and then rolling the needle in the prior art is avoided. By arranging the adjusting assembly, a user is allowed to change the length of the needle wire extending out of the needle cover by rotating the adjusting knob, so that stepless adjustment of the needle length is realized. The necessity of purchasing a plurality of microneedle devices with fixed needle lengths is eliminated, and a user can quickly adjust the needle lengths according to different treatment parts such as the face and the body or different skin conditions. The problems that according to an existing micro-needle, the functions of micro-needle piercing and liquid leading-in of bioactive substances and the like are separated, and due to the fixed structure, switching of micro-needles of different specifications is not flexible, and the use cost is high are solved.

A method for managing an individually consumable bioactive substance unit as it travels along a distribution pathway of a substance units management system, comprising: loading the substance unit into at least one container, wherein the container comprises a plurality of subsections for a plurality of substance units, wherein each subsection is configured to be associated with a single substance unit of the plurality of substance units; storing substance unit data of the substance unit with relation to the subsection accommodating it; and transferring the substance unit between subsections of the same container and / or between containers; and further storing substance unit data based on the transferring.

The invention relates to the technical field of medical instruments, in particular to an intelligent biological response regulation and control system for wound repair, which comprises a biological signal sensing module, a decision center and a regulation and control module. The biological signal sensing module monitors pH, temperature, inflammatory factor concentration and tissue fluid exudation rate of the wound surface in real time; the decision center carries out fusion analysis on the multi-source biological signals, intelligently discriminates a healing stage and generates a regulation and control instruction; the regulation and control module is used for accurately controlling the time sequence and dosage release of various bioactive substances according to instructions through a micro-fluidic chip structure. According to the invention, monitoring and intervention on wound healing are realized, and the repairing efficiency is improved.