35 results about "Hemerocallis fulva" patented technology

The invention discloses a tissue culture method of a double-petal hemerocallis fulva scapes, which comprises the following steps: taking a double-petal hemerocallis fulva scapes, taking a growing point as a center, transversely cutting into long sections which are not less than 5cm, washing and disinfecting; putting the disinfected long segments into enzymatic hydrolysate for cell wall enzymolysis, taking out the long segments after enzymolysis is completed, and washing the long segments with sterile water; transversely cutting the long section subjected to enzymolysis into small sections of 1-2cm by taking the growing point as the center; and carrying out tissue culture by taking the small sections as explants. The method applies the double-petal hemerocallis fulva variety, and has the advantages that materials can be taken at any time in the flowering phase, the tissue culture efficiency is high, and the quality is good.

The invention relates to the technical field of interplanting, in particular to a method for interplanting hemerocallis fulva under a nectariferous forest, which comprises the following steps: planning a forest stand light transmission plotting and planting zone; preparing soil layered improvement and base fertilizer; grading and planting hemerocallis fulva seedlings; erecting a dynamic shading system; drip irrigation belts are buried synchronously; synergistically pruning the nectariferous plants; root system isolation barrier construction; and dynamically monitoring and regulating the growth period. According to the method, the planting zone is precisely planned through canopy density surveying and mapping, and the optimal light environment is created for day lily; the layered soil improvement technology specifically meets the requirements of root systems with different depths; the adjustable sunshade net and the drip irrigation system form a light-temperature-water-fertilizer coordinated regulation and control network; the root prevention barrier is combined with the acid isolation belt to thoroughly block interspecific competition; accurate intervention in the whole growth period is achieved based on dynamic management of real-time data. The whole process forms a monitoring, execution and feedback closed loop, and the survival rate, the ornamental quality and the economic output of hemerocallis fulva are remarkably improved on the premise that the ecological function of the nectar forest is guaranteed.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

The invention provides a traditional Chinese medicine gel emplastrum for treating gouty arthritis and a preparation method thereof, the traditional Chinese medicine gel emplastrum for treating gouty arthritis at least comprises a gel layer loaded with a traditional Chinese medicine extract, the traditional Chinese medicine extract comprises the following raw materials: semen coicis, cortex phellodendri, radix clematidis, kadsura coccinea, rhizoma atractylodis, rhizoma dioscoreae hypoglaucae, red psychotria leaf, alangium platanifolium, kadsura pepper stem, radix aconiti, radix aconiti agrestis, semen strychni, frankincense, myrrh, angelica sinensis, safflower carthamus, radix cyathulae, radix paeoniae rubra, rhizoma smilacis glabrae, lycopodium clavatum, radices hemerocallis, weeping willow twig, evodia lepta, swamp mahogany, radix sophorae flavescentis, herba andrographitis, Chinese lobelia, coptis chinensis, edible tulip, cassia twig, pepper and borneol; a matrix of the gel layer is prepared from the following raw materials in parts by weight: silk fibroin, polyvinyl pyrrolidone, azide-polyethylene glycol-carboxylic acid, 1-ethynyl cyclopropyl carboxylic acid, 2, 5-dicarboxylic acid-3, 4-ethylenedioxythiophene and glycerol. The traditional Chinese medicine gel emplastrum disclosed by the invention can achieve a good treatment effect through percutaneous absorption.

The invention provides a breeding method of a new variety of hemerocallis fulva, and relates to the technical field of plant breeding, and the breeding method comprises the following steps: collecting pollen of three hemerocallis fulva stock seeds of hemerocallis fulva, hemerocallis fulva and hemerocallis fulva, and freezing and storing the pollen; after the hemerocallis fulva germplasm resource garden enters a flowering period, collecting pollen of a variety resource with high ornamental value and good resistance, and freezing and storing; the method provided by the invention can overcome the problem of flowering asynchronism of three stock seeds of the early flower type day lily and other horticultural day lily varieties, fully utilizes the early flowering genes of the stock seeds of the early flower type day lily, transforms the stock seeds into the filial generation through reciprocal cross, and hybridizes the three stock seeds and a plurality of horticultural varieties to obtain the hybrid variety of the early flower type day lily. A large number of novel varieties of hemerocallis fulva with various flower colors, high ornamental value and early flowering period can be obtained, and the urgent demand of the market on the novel varieties of the hemerocallis fulva can be met.

A new and distinct Hemerocallis plant named ‘Rule of Plum’ characterized by winter-hardy compact habit with clean medium-green foliage that goes dormant in the winter. The flowers are single, rounded, lightly fragrant, deep purple with a yellow-green throat. Inner tepals are reflexed with slightly crisped margins. The new plant flowers on stems up to 57 cm tall with four-way branching beginning about mid-July.

The application discloses a kind of composite plant extract and its application, skin external preparation.The raw material of the composite plant extract includes hemerocallis fulva flower and red clover, and the weight ratio of hemerocallis fulva flower and red clover is (1:5)~(5:1).The plant extract of hemerocallis fulva flower and red clover of the application can inhibit the release of histamine, thereby reducing the occurrence of inflammation and infection;The plant extract of the application cooperates synergistically, and compared with each plant alone, the histamine inhibition effect of the plant extract of the application is significantly improved;The plant extract of the application uses pure natural plant as raw material, and has low side effect and high safety;The plant raw material used in the application is widely planted in China, and the resource is easy to obtain, the price is cheap, and the cost is low.

The present invention discloses a traditional Chinese medicine composition for preventing and treating bee paralysis disease, its preparation method and application, belonging to the field of traditional Chinese medicine preparations. The traditional Chinese medicine composition, by weight, comprises the following components: 5-10 parts of Scutellaria baicalensis, 5-10 parts of Ginkgo biloba leaves, 3-8 parts of Pueraria lobata, 3-8 parts of Glycyrrhiza uralensis, 5-8 parts of Hemerocallis fulva roots, 5-8 parts of Aconitum carmichaelii, 1-5 parts of Taraxacum mongolicum, 1-5 parts of Crataegus pinnatifida and 1-3 parts of Rheum officinale. The traditional Chinese medicine composition has the effects of clearing heat and detoxifying, promoting metabolism and enhancing immunity, has outstanding prevention and treatment effects on bee paralysis disease, can increase the survival rate of infected bee colonies to more than 75%, and has safe components and small side effects.

The invention belongs to the field of plant gene breeding, and particularly relates to an MYB90 gene for regulating and controlling hemerocallis fulva anthocyanin synthesis and application of the MYB90 gene. The nucleotide sequence of the hemerocallis fulva MYB90 gene is shown as SEQ ID NO.1, the amino acid sequence of the hemerocallis fulva MYB90 gene is shown as SEQ ID NO.2, it is found for the first time that the hemerocallis fulva MYB90 gene can regulate and control synthesis of anthocyanin in tobacco flowers, and colors of tobacco leaves, stems, flowers and other positions can be deepened through overexpression of the hemerocallis fulva MYB90 gene. The hemerocallis fulva MYB90 gene provided by the invention not only provides a theoretical reference for research of hemerocallis fulva flower color regulation, but also provides an important gene resource for future flower color breeding work.

The hemerocallis fulva planting and irrigating equipment comprises a bottom plate, a plurality of moving wheels which are evenly distributed are arranged at the bottom of the bottom plate, a water tank is fixedly connected to the top of the bottom plate, a connecting box is fixedly connected to the top of the water tank, and a water pump is fixedly connected to the interior of the water tank. Through the effect of the designed filter screen, rainwater can be filtered, impurities carried in the rainwater can be prevented from entering the rainwater, the influence on subsequent rainwater conveying is prevented, the filtered rainwater can enter the water tank to be collected, subsequent irrigation use is facilitated, in the filtering process of the filter screen, the electric push rod intermittently works, and the rainwater collecting efficiency is improved. The output end of the electric push rod can drive the sliding block to move, the sliding block can drive the moving block to move, the hinged rod and the filter screen can deflect to enable the filter screen to incline, impurities accumulated on the surface of the filter screen can fall down along the inclined filter screen, and it is avoided that the filter screen is blocked, and rainwater collection is affected.

This invention relates to a novel substance with in vitro anti-inflammatory activity, its preparation method, and its application. The present invention provides a novel substance with in vitro anti-inflammatory activity, its preparation method, and its application. The molecular formula of hemerocallicin A is C2. 23 H 28 O 10 Methods: 1. Hemerocallis flowers were extracted with 80% ethanol aqueous solvent; 2. The concentrated extract after removing ethanol by rotary evaporation was dispersed in water and loaded onto a D101 macroporous resin column; 3. The fraction was eluted with 50% ethanol from the macroporous resin, and then purified by silica gel column chromatography, gel column chromatography, and preparative high-performance liquid chromatography to obtain hemerocalin A. Hemerocalin A is used to prepare anti-inflammatory drugs. The IC50 of hemerocalin A in this invention inhibited the release of the pro-inflammatory factor NO from LPS-stimulated mouse microglia (BV2) by [the method described in the original text]. 50 The value was 13.3 μmol / L, indicating that it has good in vitro anti-inflammatory activity. This invention belongs to the field of research on effective components of natural drugs.

The invention relates to the field of plant breeding, and particularly discloses application of chrysanthemum MYB-A protein to regulation and control of flower color of day lily, a cultivation method of the chrysanthemum MYB-A protein and a related biological material. According to the application, the MYB-A gene is cloned from chrysanthemum, a recombinant plant expression vector is successfully constructed, and the recombinant plant expression vector containing the chrysanthemum MYB-A gene is transformed into day lily by adopting an agrobacterium transformation method. Researches show that overexpression of the MYB-A gene in hemerocallis fulva remarkably affects the flower color of the hemerocallis fulva, so that the flower color of a transgenic plant is darkened or mottle phenomenon occurs. The discovery proves that the chrysanthemum MYB-A gene plays an important role in regulating and controlling the flower color of the hemerocallis fulva, the effective regulation and control on the flower color of the hemerocallis fulva are realized, and the flower color characteristics of the hemerocallis fulva can be accurately changed under the condition of not depending on traditional hybridization or mutation breeding.

The utility model discloses a slurry injection molding device for processing crispy hemerocallis fulva and hippophae rhamnoides, and relates to the field of food processing. Comprising a working table, a discharging port is formed in the working table, a fixing base and a fixed mold assembly are fixedly installed on the working table, the fixed mold assembly comprises a fixed mold body fixed to the discharging port, iron mold cavities are formed in the fixed mold body, and high-frequency heating coils are fixedly installed on the outer sides of the iron mold cavities. According to the slurry injection molding device for processing the hippophae rhamnoides brittleness, after a power supply of the high-frequency heating coil is switched on, the high-frequency heating coil can synchronously heat the iron mold cavity in the fixed mold body, so that hippophae rhamnoides slurry in the iron mold cavity is heated, and part of the hippophae rhamnoides brittleness is prevented from being adhered in the mold; before mold closing, the edible oil nozzle sprays out edible oil, and the sprayed edible oil forms an oil film on the iron mold cavity and the mold pressing block, so that the hippophae rhamnoides brittle adhesion in the mold is further prevented.

The invention discloses a preparation method of hemerocallis fulva tea, which mainly comprises the following steps: fresh flower picking, rolling processing, fermentation processing and baking processing, the technology discloses core key parameters of the whole process from the harvesting of hemerocallis fulva flowers to the preparation of fermented black tea through an optimal manufacturing process of tests; the technical bottleneck breakthrough of development of the hemerocallis fulva flower black tea beverage is realized, and technical guidance is provided for industrial development and production of the hemerocallis fulva flower black tea in the future; the method comprises the steps of fresh flower picking, rolling processing, fermentation processing, baking processing and hemerocallis fulva black tea making, the making technology and condition parameter setting are scientific and reasonable, and the method is an optimal scheme for making the hemerocallis fulva black tea. The dried day lily black tea is dark black in color and luster, has strong fragrant and sweet smell, has caramel taste but does not have burnt taste after being smelled, and is red and bright in tea soup, mellow and sweet in taste and moderate in caramel taste after being brewed with boiled water.

The invention discloses yellow yellow wine and a preparation method thereof, and belongs to the technical field of wine brewing. The method comprises the following steps: by taking broomcorn millet as a raw material, soaking, cooking congee, mixing with yeast, carrying out main fermentation, carrying out secondary fermentation, adding vacuum freeze-dried yellow pollen after the secondary fermentation is completed, and then carrying out after-ripening, squeezing, decocting wine, aging and storing and finishing. According to the method, active ingredients in the day lily are fully dissolved out and stably retained by optimizing the day lily adding time and the after-ripening process. In the obtained yellow yellow wine, the total phenol content is not lower than 0.50 mg / mL, the flavonoid content is not lower than 0.12 mg / mL, the vitamin C content is not lower than 11 mu g / mL, the hydroxyl radical scavenging rate is not lower than 85%, the amino acid content is not lower than 3300 mu g / mL, and the yellow yellow wine has remarkable antioxidant activity and enhanced nutrition flavor.

The utility model discloses a day lily air-drying placing device which comprises a first supporting piece which is movably connected with two sets of second supporting pieces. The driving assembly is used for driving the two sets of second supporting pieces to rotate so as to achieve angle adjustment; according to the day lily air-drying device, it is guaranteed that when the wind direction is changed, the angle is adjusted in time, sufficient air circulation is guaranteed, mildewing is prevented, the air-drying time is shortened, limitation of a traditional fixing support is broken through, flexible adjustment can be achieved along with environment changes, and the day lily air-drying device is convenient to use and high in practicability. Natural air-drying conditions are fully utilized, and the air-drying efficiency is improved.

The invention belongs to the technical field of bioengineering, and particularly relates to application of a detection reagent of a molecular marker related to flower color traits of hemerocallis L. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, an SNP site exists at the 44bp position from the 5'end of the sequence, the polymorphism is T / C, and CC and TC genotypes are shown. The invention also develops a reagent for detecting the molecular marker, and the reagent specifically comprises a forward primer with nucleotide sequences as shown in SEQ ID NO.2 and SEQ ID NO.3 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.4. The reagent can be used for breeding the hemerocallis plants, breeding the hemerocallis plants with different flower color characters or identifying the flower color characters; the invention also develops a flower color character identification method, which is beneficial for accelerating the genetic breeding of the hemerocallis plants.

The invention discloses a composition with anti-wrinkle and firming effects. The composition is prepared from the following components: a hemerocallis fulva flower extract, a lilium davidii linn / leaf extract, a fructus chebulae bark extract, a magnolia liliiflora bud extract, a phalaenopsis aphrodite extract, butanediol, hexanediol and water. The composition is safe, stable, efficient and mild, formed wrinkles and relaxation problems can be remarkably improved, new wrinkles can be prevented, through the synergistic effect of plant components, the irritation is greatly reduced while the effect is guaranteed, and sensitive skin can also safely use efficient anti-aging products.

The invention relates to the field of plant breeding, and particularly discloses application of a chrysanthemum MYB-B transcription factor in regulating and controlling the filament length of hemerocallis fulva, a cultivation method and a biological material. According to the application, the MYB-B gene is cloned from chrysanthemum, a recombinant plant expression vector is successfully constructed, and the recombinant plant expression vector containing the chrysanthemum MYB-B gene is transformed into day lily by adopting an agrobacterium transformation method. As the chrysanthemum MYB-B transcription factor has a specific amino acid sequence, the chrysanthemum MYB-B transcription factor can specifically participate in and influence the growth process of hemerocallis fulva filaments so as to regulate and control the lengths of the filaments. The regulation and control effect is favorable for optimizing the structural characteristics of the hemerocallis fulva flowers, so that filaments are lengthened, the exposure degree of anther and pollen is improved, the pollination efficiency is further improved, and positive influences are brought to the ornamental value and the reproductive capacity of the hemerocallis fulva.

Disclosed are a solid product for preparing a Hemerocallis citrina extract, and a preparation method therefor. The method comprises the following steps: A) carrying out enzymatic hydrolysis on raw mat

The invention belongs to the technical field of ice cream processing, and discloses day lily ice cream as well as a preparation method and application thereof, and raw materials for preparing the day lily ice cream comprise day lily powder, milk powder, ice cream powder and the like in a specific weight ratio. According to the day lily ice cream, day lily powder is prepared by adopting a vacuum freeze-drying process based on color protection of L-ascorbyl palmitate, then the day lily powder is uniformly mixed with other raw materials, and the day lily ice cream is prepared by adopting an ultra-micro emulsification process and adjusting the expansion rate of the ice cream. The product has the effects of dense texture, good stability, unique hemerocallis citrina baroni flavor, health, no toxicity and the like. The hemerocallis fulva ice cream can be used for preparing food such as pastries and drinks containing ice cream. The method is used for producing and processing the flavored ice cream.

The invention belongs to the technical field of plant breeding, and particularly relates to a method for increasing the germination rate of hemerocallis fulva seeds. According to the method, the germination rate of the hemerocallis fulva seeds can be increased by harvesting, storing and treating the seeds to break dormancy and accelerating germination, the time is short, the operation is simple, the germination rate can be increased to 86% or above, the technical problem of seed germination in new variety breeding of hemerocallis fulva is thoroughly solved, and a powerful technical guarantee is provided for hemerocallis fulva breeding.

A new and distinct cultivar of Hemerocallis plant named ‘G221407’ is disclosed, characterized by flowers of an apricot-orange color, with a yellow throat, a darker orange-red eye and a slight ruffle to the petal edge. ‘G221407’ is a diploid, dormant type daylily, losing foliage with frost each fall, and re-emerging from the crown in the following spring. ‘G221407’ grows quickly and vigorously following emergence from dormancy, showing a growth rate during bare-root production which exceeds most comparable daylilies. ‘G221407’ blooms continuously from first-flower in early June until hard frost in the autumn. Plants are observed winter-hardy through at least USDA Zone 5. The new variety is a Hemerocallis, normally used for outdoor ornamental purposes.

The utility model discloses a hemerocallis fulva seed screening device for agricultural processing, which belongs to the technical field of agricultural processing and comprises a bottom frame, a supporting frame is fixedly connected to the top of the bottom frame, a top frame is fixedly connected to the top of the supporting frame, a mounting frame is fixedly connected to the bottom of the top frame, and a supporting assembly is arranged on the inner side of the top frame. The hemerocallis fulva seed screening device for agricultural processing has the beneficial effects that a driving motor works to drive a supporting plate to rotate, so that a first supporting shaft is driven to rotate, a first sleeve, a connecting plate, a second sleeve and a second supporting shaft are matched, a fixing frame and an adjusting frame can be pushed to move, and meanwhile by means of limiting supporting of a supporting assembly, the screening efficiency is improved. The movement is limited, the driving motor works, the adjusting frame and the filtering frame can repeatedly move back and forth in the limited direction, seeds and impurities can be driven to roll and flow, and the seeds can be continuously and repeatedly screened in cooperation with the filtering frame.

This invention discloses a method for tissue culture of double-flowered daylily scapes, comprising: taking the scape of a double-flowered daylily variety, transversely cutting it into segments no less than 5 cm long with the growing point as the center, and rinsing and disinfecting the segments; placing the disinfected segments into an enzymatic hydrolysis solution for cell wall enzymatic hydrolysis, removing the segments after hydrolysis, and rinsing them with sterile water; transversely cutting the hydrolyzed segments into 1-2 cm segments with the growing point as the center; and using the segments as explants for tissue culture. This method, using double-flowered daylily varieties, has the advantages of allowing for the collection of materials at any time during the flowering period, resulting in high tissue culture efficiency and good quality.

This invention relates to the field of cosmetic technology, providing a barrier repair composition for dry and sensitive skin containing purslane extract and its application. The composition comprises purslane extract, Bletilla striata extract, Hemerocallis fulva extract, ceramide complex lipids, and ectoine. This invention uses purslane as a soothing and stabilizing base, combined with Bletilla striata for film-forming repair, Hemerocallis fulva for soothing repair, barrier lipid replenishment, and anti-stress components, forming a multi-pathway synergistic care system that can improve dry and sensitive skin problems such as dryness, tightness, stinging, itching, flaking, fine cracks, barrier fragility, and seasonal discomfort. Experimental results show that the composition of this invention is mild and stable, suitable for industrial production and daily dry and sensitive skin care.

The utility model discloses a sunlight drying day lily pretreatment device which comprises a cleaning box, a support is fixedly installed on the rear side of the cleaning box, an electric push rod is fixedly installed on one side of the support, a lifting plate is installed at the output end of the electric push rod, a rotating block is rotationally connected to the bottom face of the lifting plate, and a transverse plate is fixedly installed on the bottom face of the rotating block. A net plate is installed on the bottom face of the transverse plate, a liftable containing assembly is installed on the bottom face of the transverse plate, a connecting block is fixedly installed on the top face of the transverse plate, a limiting hole is formed in the surface of the connecting block, and a lifting assembly penetrating through the inner side of the limiting hole is installed on the top face of the lifting plate. The electric push rod can drive the lifting plate, the rotating block, the transverse plate and the containing assembly to ascend and descend, and the lifting assembly can drive the transverse plate and the containing assembly to swing, so that impact of water on hemerocallis fulva in the containing assembly is enhanced, and the cleaning effect is guaranteed.

The invention discloses a tissue culture method of hemerocallis middendorfii'white swan '. The method comprises the following steps: (1) obtaining a sterile material; (2) callus induction; (3) bud differentiation; (4) rooting culture; and (5) seedling hardening and transplanting. According to the method, underground buds serve as explants, complete plants are cultured through a series of culture medium formulas including explant disinfection, callus induction, bud differentiation and rooting culture, seedlings can be formed after 1-2 months of seedling hardening and transplanting, and the survival rate reaches 98%. The tissue culture method of the hemerocallis middendorfii'white swan 'is efficient and rapid, and lays a foundation for rapid propagation, popularization and application of the hemerocallis middendorfii.

The invention discloses a tissue culture method of root stocks of hemerocallis fulva, which comprises the following steps: step 1, selecting a robust root stock of a hemerocallis fulva variety as an explant, transversely cutting the root stock into small sections, and cleaning and disinfecting the small sections; 2, the disinfected explant is longitudinally cut under the sterile condition and then rapidly transferred to an MS solution containing 0.4-0.6 mg / L of 6-BA and 0.08-0.12 mg / L of NAA to be soaked for 30 min, the temperature of the solution is controlled to be about 20 DEG C, and then the explant is inoculated to an induction culture medium in an upright arrangement mode to induce adventitious buds; step 3, inoculating the adventitious buds on a proliferation culture medium in a single plant or cluster bud mode for proliferation; 4, dividing the adventitious buds into single plants, and inoculating the single plants into a rooting culture medium to induce rooting; 5, after the rooting seedlings are hardened, transplanting is conducted, and hemerocallis fulva plants are obtained. The method applies the double-petal hemerocallis fulva variety, and has the advantages of being capable of obtaining materials all year round, high in propagation coefficient and the like.

The invention belongs to the technical field of agricultural breeding, and particularly relates to a day lily doubling method. Through a ploidy breeding method, a group culture seedling induced by an explant is used for treatment, a hemerocallis fulva hybridized seed sowing seedling is directly subjected to double induction or a hemerocallis fulva field seedling is treated, so that the hemerocallis fulva is doubled. The result of the embodiment shows that the success rate of the hemerocallis fulva doubling method is 100%, the doubling efficiency is high, the seedling loss is small, and a powerful technical guarantee is provided for hemerocallis fulva ploidy breeding.