158results about "Hybrid cell preparation" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing isopentene aromatic compounds as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, synthesis of two precursors of p-coumaric acid and dimethyl aryl pyrophosphate is engineered in parallel in two haploid yarrowia lipolytica strains. And then mating the receptor strain with the donor strain to construct the diploid capable of efficiently synthesizing the drpanin and the artepillin C. The invention further discloses a preparation method of the diploid of the drpanin and the artepillin C. By using a similar method, a strain capable of producing the 8-prenylnaringenin is also constructed. According to the present invention, the basis is provided for the sustainable production of drpanin, artepillin C and 8-prenylnaringenin, and the new way is provided for the rapid engineering of the yeast to produce other isopentenylated compounds.

Agriculturally useful genetically engineered bacteria which express RNAs or proteins that produce agriculturally useful compounds including ammonia, phosphate, and pesticides upon decreases in phosphate concentrations in plant growth media and methods of their use are disclosed.

The present invention discloses a sporeless Pleurotus ostreatus strain 3H02F79 and a breeding method thereof, relating to the fields of edible fungi and molecular marker-assisted breeding technology. The 3H02F79 strain, deposited with CCTCC NO: M 2025718, exhibits superior agronomic traits to its sporeless parent, QHP315. Its spore production is sporeless, resulting in an 18.18% increase in yield compared to its sporeless parent, QHP315. Compared to its commercial spore-forming parent, P2101, it eliminates the harmful effects of spore release and exhibits no significant differences in mycelial growth rate or yield. Compared to its two parent strains, the 3H02F79 strain has the lowest contamination rate and the shortest tide-turning time. Furthermore, through plot and expanded trials, the 3H02F79 strain has demonstrated a 100% fruiting rate, stable properties, and highly consistent phenotypes across different plots, making it a highly promising sporeless cultivar for industrial cultivation.

The invention discloses a preparation method and application of a tetraploid saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. Comprising the following steps: (1) knocking out an HO gene of diploid yeast LFN524, separating haploids through spore production, and identifying mating types of candidate haploid strains to obtain haploid strains with opposite mating types of haploids; (2) respectively culturing haploid strains of different mating types, inducing fusion of the haploid strains, and screening successfully fused diploid strains; and (3) inducing the successfully fused diploid strain to fuse again to obtain the tetraploid saccharomyces cerevisiae strain. The nucleic acid content of the strains 8-3 and 8-4 disclosed by the invention is obviously higher than that of a diploid stock plant, and the nucleic acid content can respectively reach 0.118 g / g-DCW and 0.096 g / g-DCW, which are respectively increased by 63.89% and 33.33%.

The invention belongs to the technical field of bioengineering, and relates to industrial microorganism breeding, in particular to a high-yield ethyl acetate diploid / tetraploid saccharomyces cerevisiae strain as well as a construction method and application thereof. According to the research, a diploid / tetraploid saccharomyces cerevisiae strain is constructed on the basis of an existing high-yield ethyl acetate haploid yeast strain by utilizing a CRISPR / Cas9 mediated mating type conversion technology. And a series of diploid / tetraploid saccharomyces cerevisiae strains with the acetic acid and lactic acid tolerance and the ethyl acetate yield obviously improved compared with the starting strain are obtained. According to the series of strains, ethyl acetate is high in yield while the excellent alcoholic fermentation characteristic is kept in the fermentation process, white spirit fermented by the series of strains is more prominent in ester fragrance and grain fragrance, weaker in foreign flavor and better in taste on the basis of guaranteeing the original style, a new thought is provided for the breeding mode of yeast strains, and the method is worthy of popularization and application. And a reference is provided for production optimization of ethyl acetate in a Baijiu fermentation process.

Provided herein is a conditionally-replicating serotype 5 adenovirus or adenoviral vector expressing a mutant E1A protein under control of a promoter that is responsive to hypoxia and inflammation and one or more immune modulators under control of a tumor-specific promoter. The adenovirus or adenoviral vector also comprises serotype 3 fiber domain. Also provided is a method of inducing cytotoxicity in tumor cells using a composition containing the adenovirus or adenoviral vector.

The invention relates to application of acremonium terricola F2652 in preparation of a preparation for improving the production performance, immunity or antioxidation of weaned piglets, and belongs to the technical field of strain fermentation. The contents of active components such as cordycepin, polysaccharide and the like in secondary metabolites of the acremonium terricola F2652 are obviously increased, so that the conversion and enrichment of the polysaccharide of the acremonium terricola strain can be realized while the content of a symbolic component cordycepin of cordyceps fungi is increased. The acremonium terricola F2652 is subjected to liquid submerged fermentation to obtain a fermentation culture, the advantages of amphiphilic parents are fused, the excellent compound polysaccharide synthesis capacity is reserved, the good cordycepin accumulation potential is also fused, and the acremonium terricola culture with the high cordycepin content can be developed; the practical application of the feed additive as a functional feed additive in different animals can be enriched.

A method of alignment and fusion of cells with an electrical field includes firing a first fluid dispenser of an electrofusion device until a first impedance sensor in a first microfluidic channel of the electrofusion device detects a presence of a first cell. The method includes firing a second fluid dispenser of the electrofusion device until a second impedance sensor in a second microfluidic channel of the electrofusion device detects a presence of a second cell. The method includes moving the first cell and the second cell into a merging chamber of the electrofusion device by firing a third fluid dispenser of the electrofusion device, in response to alignment of the first cell in the first microfluidic channel with the second cell in the second microfluidic channel. The method further includes fusing the first cell and the second cell in the merging chamber by creating an electrical field in the merging chamber.

The invention belongs to the technical field of biosynthesis, and discloses a method for improving the yield of valarene by using a saccharomyces cerevisiae rearrangement technology, which comprises the following steps: firstly, constructing a saccharomyces cerevisiae rearrangement strain library by using a synthetic chromosome rearrangement technology; then, the haploid strains in the saccharomyces cerevisiae rearrangement strain library and the valarene haploid production strains are mated to generate diploid valarene production strains; and finally, fermenting the diploid valarene production strain to obtain a valarene product. Fermentation verification is carried out on a diploid ceraene production strain and a haploid ceraene production strain (contrast), and results show that the yield of the ceraene haploid production strain is 163.81 mg / L, the highest yield of the diploid production strain is 194.14 mg / L, the yield is improved by 18.5% compared with that of an original strain, and it is proved that the production capacity of the ceraene is improved through the method.

The invention belongs to the technical field of edible mushroom biological breeding, and particularly discloses a Stropharia rugoso-annulata breeding method based on Tricholoma giganteum and Stropharia rugoso-annulata protoplast fusion, which comprises the following steps: respectively preparing Stropharia rugoso-annulata protoplast and Tricholoma giganteum protoplast; performing thermal inactivation on the stropharia rugoso-annulata protoplast, and performing ultraviolet inactivation on the tricholoma giganteum protoplast; mixing the two inactivated protoplasts, and adding an induction reagent for protoplast fusion; coating the fusion product on a regeneration culture medium for culturing to obtain a fusion strain; screening a target fusion strain through an antagonism test and a molecular marker technology; fruiting management is carried out, and a new high-temperature-resistant stropharia rugoso-annulata strain is screened out. By optimizing protoplast preparation and PEG fusion promotion conditions, the distant fusion efficiency is remarkably improved, and a novel stropharia rugoso-annulata strain which can normally fruit at the high temperature of 30 DEG C and is excellent in agronomic character and good in flavor is successfully obtained in combination with an efficient screening and identification system.

The present invention discloses a fusion strain of Lactobacillus paracasei and Bifidobacterium adolescentis, as well as its preparation method and application, belonging to the field of strain breeding technology. A fusion strain of Lactobacillus paracasei and Bifidobacterium adolescentis was successfully prepared using the method provided by the present invention. The prepared fusion strain has a new morphology, a broadened carbon source utilization range, and significantly improved inhibitory effects on three pathogens. Furthermore, it is resistant to both vancomycin and mupirocin lithium salts. It also has good fermentation characteristics, with a pH of 4.05 and a titer of 114°T after 28 days of storage, and the viable cell count can still be maintained at 1×10 7 The protoplasts have a CFU / mL value of above 2260 mPa·s, a viscosity value of 37.462 g, a hardness of 37.462 g, and a consistency of 282.04 g·s, and can be used for the development and application of dairy products. The preparation method provided provides protoplasts with strong regeneration ability, high vitality, and high fusion rate, providing support for the selection of excellent lactic acid bacteria strains using protoplast fusion technology.

The present invention relates to a method for transforming a strain of the Lactococcus genus through natural competence. The present invention further relates to strains obtained or obtainable by said method. The present invention also relates to a method for identifying a strain of the Lactococcus genus which is transformable through natural competence.

The present disclosure is directed to genetically modified bacteria that produce a diffusible signal factor (DSF). The disclosure also provides methods for treating or preventing Salmonella infection / invasion in a subject. The disclosure further provides vectors and compositions for carrying out the methods disclosed herein.

This disclosure provides a technology for adapting host cells to maximize production of viral vectors and particles. Cell hybrids are formed from parental cell lines, and divided or cloned into multiple aliquots for testing. Aliquots are chosen that have high production capacity and phenotypic features for virus production, such as an optimal level of intracellular organelles, and used to establish producer cell lines. The producer cells can be genetically altered to express a transgene that encodes viral elements for production of the viral vectors or particles with a therapeutic payload. The hybrid producer cells generate more viral vectors or particles per cell with higher functional titer, thereby lowering the cost of production of pharmaceutical agents for use in gene therapy and immunization.

The present disclosure relates to a novel isolated binding protein including a N-terminal pro-brain natriuretic peptide (NT-proBNP) antigen binding domain, and athe preparation method therefor. The antigen-binding domain includes at least one complementarity determining region selected from the amino acid sequences as defined in the present disclosure: or; has at least 80% sequence identity with the complementarity determining region of the following amino acid sequence and has an affinity of KD≤2.26×10-8 to NT-proBNP. The binding protein may be used in the detection field of NT-proBNP protein.

The invention discloses a bacterial outer membrane mixed vesicle carrier, the mixed vesicle is a mixed membrane structure formed by fusing two heterotype bacterial outer membrane vesicles, and the two heterotype bacteria are respectively gram-positive bacteria and gram-negative bacteria. The mixed vesicle carrier can simulate a natural internalization path of pathogenic bacteria (G < + > / G <->) invading cells, accurately locks cells which are easily invaded by bacteria, can identify corresponding homologous bacteria surfaces, efficiently enriches drugs at bacterial infection parts, and effectively improves treatment on mixed bacterial infection parts.

This disclosure provides anti-5T4 antibodies, variants thereof and humanized versions. The newly disclosed antibodies exhibited high affinity to the 5T4 protein and can be used to treat cancers.

This disclosure provides, among other things, strategies for minimizing antibody diversification in a transgenic animal that uses gene conversion for antibody diversification. In some embodiments, the animal may comprise a genome comprising an endogenous immunoglobulin light chain locus comprising: (a) a functional immunoglobulin light chain gene comprising a nucleic acid encoding a light chain variable region; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin light chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding a light chain variable region, wherein the pseudogenes are upstream or downstream of the functional immunoglobulin light chain gene and encode the same amino acid sequence as the light chain variable region of the functional immunoglobulin light chain gene of (a). In other embodiments, the locus may have a tandem array of coding sequences for the light chain.

The invention discloses a hybrid vesicle for regulating purine metabolism and a preparation method and application thereof.The hybrid vesicle is formed by fusing extracellular vesicles derived from macrophages and extracellular vesicles derived from endothelial cells, CD39 and CD73 are arranged on membranes of the hybrid vesicle at the same time, purine metabolism can be efficiently and stably catalyzed, and pro-inflammatory ATP is converted into ADO with the immunoregulation function; the preparation method of the hybrid vesicle is simple and suitable for mass production; the application of the hybrid vesicle is helpful for overcoming the limitation of the existing autoimmune disease treatment method, improving the accuracy and effectiveness of autoimmune disease treatment and reducing the side effect of treatment, and has important clinical application value and wide market prospect.

The invention belongs to the food industry, relates to a novel method for producing casein compositions by culturing transgenic microorganisms in the presence of whey, and limits the environmental impact.

A system for expressing a chloramphenicol split protein is disclosed. Uses thereof are also disclosed.

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and / or reducing carbon losses resulting from the production of byproducts.

Provided are compositions and methods for prophylaxis and / or therapy of a variety of cancers which express a Claudin 6 (CLDN6) antigen. Included are recombinant T cell receptors (TCRs), polynucleotides encoding them, expression vectors that include the polynucleotides, and cells into which the polynucleotides have been introduced to produce modified cells, including CD4+ T cells, CD8+ T cells, and progenitor cells, such as hematopoietic stem cells. The modified cells are capable of direct and indirect recognition of a cancer ell expressing a CLDN6 antigen by human leukocyte antigen (HLA) class I and II restricted binding of the TCR to the CLDN6 antigen expressed by the cancer cell, with or without presentation of the antigen by antigen presenting cells. Also included is a method for prophylaxis and / or therapy of cancer by administering modified cells that express a recombinant TCR that binds to CLDN6.

The present invention relates to microorganisms, genes, materials and methods for improving gene competence. In particular, the present invention provides individual genes / proteins and combinations thereof for improving mating competence in Firmicutes, as well as methods and uses comprising such genes, proteins and respective combinations.

The present disclosure is related to compositions and methods comprising genetically modified Arthrospira platensis strains that express methanogen-killing lytic enzymes (methanolysins) to inhibit methane emission produced by enteric archaea.

Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase / isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The invention provides an Isaria cicadae Miquel hybrid strain with high yield of spore powder and a preparation method thereof, the Isaria cicadae Miquel hybrid strain with high yield of spore powder is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.65980, and the ITS sequence of the Isaria cicadae Miquel hybrid strain with high yield of spore powder is as shown in SEQ ID NO.1. The cordyceps sobolifera conidial powder collected and separated from Shoushan of Taiwan and Wuyi Mountain of Fujian is hybridized to breed the cordyceps sobolifera hybridized strain with high yield of conidial powder, so that the breeding period of the cordyceps sobolifera is greatly shortened. The hybrid strain inherits excellent characters of parent cordyceps sobolifera in Wuyi Mountain of Fujian and cordyceps sobolifera in Shoushan of Taiwan, not only can generate a large number of spores, but also has the characteristics of early grass growth, good regularity, early spore production, high yield and the like. According to the cordyceps sobolifera hybrid strain, the conidial powder content is remarkably increased, and then the breeding efficiency is improved.