677 results about "Ovary" patented technology

The invention relates to the technical field of biomarkers, in particular to a blood biomarker combination for prediction and auxiliary diagnosis of ovarian reserve hypofunction (DOR), a corresponding detection kit and application of the blood biomarker combination. According to the invention, the content change of the nicotinamide mononucleotide (NMN) and the metabolite combination thereof in serum or plasma is screened for the first time and can be used as a detection index for predicting the ovarian reserve function decline. Based on the biomarker combination, the invention further provides an in-vitro detection kit containing a related standard substance, an internal standard solution and an extracting solution, and a data analysis method combined with a random forest model, so that an individual with a normal ovarian reserve function and an individual with hypofunction can be accurately distinguished. The method is noninvasive, simple, convenient, high in sensitivity and suitable for early recognition and layered screening of clinical ovarian reserve hypofunction.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention discloses sturgeon ovary active peptide with ovary aging resistance and a preparation method of the sturgeon ovary active peptide. According to the invention, sturgeon ovary is taken as a raw material, and peptide with the highest ovarian aging resistance activity is selected from different enzymolysis combinations through an in-vitro anti-oxidation test and an ovarian granular cell and caenorhabditis elegans test; three sturgeon ovary active peptide sequences with ovary senescence resistance activity are screened by virtue of bioinformatics technologies such as mass spectrum identification, molecular docking and the like, and the amino acid sequences of the sturgeon ovary active peptide sequences are Phe-Met-Ile (FMI), Phe-Asp-Pro-Phe (FDPF) and Ser-Trp-Pro-Trp-Gln (SWPWQ). The sturgeon ovarian peptide prepared by the method not only has remarkable anti-premature ovarian failure activity and successfully solves the problem of high-valued utilization of sturgeon byproducts, but also can be used as a natural and efficient functional factor, is widely applied to functional products in the related fields of health care products, cosmetics, medicines and the like, and has a wide application prospect. And the method shows extremely wide market application prospects and huge economic values.

The present disclosure provides a Chinese Hamster Ovary (CHO) cell comprising one or more exogenous nucleotide sequences, wherein the one or more exogenous nucleotide sequences is inserted into a chromosomal locus of the CHO cell, wherein the chromosomal locus is located within contig NW_023276805.1 of chromosome X. Also provided herein are methods of producing a CHO cell capable of expressing a sequence of interest, methods of expressing a sequence of interest comprising culturing the CHO cell as described herein, and kits comprising the CHO cell as described herein.

The embodiment of the invention discloses an ovarian health assessment method and related equipment, the method is applied to first electronic equipment, the first electronic equipment can display a first interface, and the first interface comprises a first control; receiving a first operation acting on the first control; acquiring first data, wherein the first data comprises heart rate variability data of the user in a preset time period; according to the physical sign data of the user, determining an ovary health assessment result; the user sign data comprises first data; the ovarian health assessment result is used for indicating the ovarian health condition of the user in a preset time period; after the preset time period, a second interface is displayed, and the display content of the second interface comprises the ovarian health assessment result. By adopting the method, the ovarian function abnormity of the user can be found in time.

The invention relates to the technical field of biomedical treatment, in particular to bifidobacterium longum subsp. Longum and application thereof in drugs related to reproductive system diseases. The invention provides a strain of Bifidobacterium longum subsp. Longum NCU-06, which is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is January 18, 2023, and the preservation number is CGMCC No.26491. The invention also provides a method for preparing the Bifidobacterium longum subsp. Longum NCU-06. The bifidobacterium longum subsp. Longum NCU-06 has a treatment effect on various female reproductive system diseases, for example, the bifidobacterium longum subsp. Longum NCU-06 can be used for treating endometriosis, ovarian cancer, ovarian cancer parneoplastic thrombocythemia and ovarian hyperstimulation syndrome.

The invention belongs to the technical field of medicines, and particularly relates to a composition containing mesenchymal stem cells and application of the composition in preparation of products for resisting premature ovarian failure. The invention relates to a composition containing mesenchymal stem cells. The umbilical cord mesenchymal stem cell gel is prepared from the following components in percentage by weight: 10 percent of umbilical cord mesenchymal stem cells, 4 to 6 percent of human serum albumin stock solution, 1 to 2 percent of ginkgo biloba extract, 1 to 3 percent of coix seed oil, 2 to 2.5 percent of flavonol glycoside, 1.1 to 1.3 percent of vitamin E, 0.4 to 0.8 percent of glutathione, 0.5 to 1.5 percent of sodium hyaluronate, 0.5 to 1.0 percent of phycocyanin, 1.2 to 1.8 percent of zinc gluconate, 0.5 to 0.8 percent of heparin calcium, 0.1 to 0.3 percent of pH regulator and the balance of normal saline. The composition disclosed by the invention can be used for preparing products for resisting premature ovarian failure and has a very good effect.

The invention discloses an ovary-targeting mRNA liposome nanoparticle, a preparation method, a drug compound, a targeting delivery system and application, and relates to the technical field of liposome granules, the ovary-targeting mRNA liposome nanoparticle comprises the following components by mass: 20%-80% of SM-102, 1%-20% of PEG2000, 5%-40% of CHO-HP, 1%-20% of DSCP, and 0.01%-10% of targeting polypeptide, the target polypeptide comprises at least one of the following sequences: LVYKDPARPNTQK, RCGPCRLSSSDCGGPRTQPMACDLP, and CALCRRSTSDCGGPKDHPLT (recombinant human serum albumin), and the target polypeptide comprises at least one of the following sequences. The present invention provides a liposome nanoparticle for targeted ovary and uterus delivery of mRNA. The lipidosome nano-particles are specially researched, developed and designed for mRNA, have a good targeted delivery effect in mRNA in-vivo delivery, and can be applied to the field of targeted drug delivery of ovaries and uteruses. When in use, the kit can be directly prepared with a specific mRNA sequence for use, so that different application requirements can be flexibly met.

The invention provides a traditional Chinese medicine composition for treating ovarian reserve function decline. The traditional Chinese medicine composition is prepared from the following medicines in parts by mass: 9-30 parts of radix astragali preparata, 6-20 parts of roasted rhizoma atractylodis macrocephalae, 10-15 parts of poria cocos, 2-10 parts of radix glycyrrhizae preparata, 6-20 parts of semen cuscutae, 9-20 parts of radix dipsaci (dipsacus root), 6-12 parts of herba lycopi and 6-15 parts of lily. Experimental results show that the traditional Chinese medicine composition is suitable for improving the ovarian function of a patient suffering from spleen-kidney deficiency type ovarian reserve function decline.

The invention belongs to the technical field of biology, and particularly relates to a receptor peptidomimetic capable of improving the breeding efficiency of butter crabs and application of the receptor peptidomimetic. The optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hotspot residues are screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hotspot residues is calculated to determine the receptor peptidomimetic sequence. The receptor peptidomimetic can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and the receptor, so that the butter crab product can be produced in a planned manner.

The present invention belongs to the technical field of plant extracts, and more specifically, relates to the use of a carob extract in a drug for improving glucose metabolism / polycystic ovary syndrome in women. The present invention discloses a pharmaceutical composition for improving glucose metabolism and treating polycystic ovary syndrome in women, the pharmaceutical composition comprising: carob extract, baicalein, cedrol, a bioactive peptide, a biopolysaccharide, and excipients. The composition of the present invention has the effects of lowering blood sugar and blood lipids, improving insulin function, and also has antioxidant and immunity-enhancing effects. By combining the above substances, a synergistic effect is achieved, further enhancing the effect of improving / treating polycystic ovary syndrome in women associated with abnormal glucose metabolism.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention discloses a method for efficient maturation of ovaries of industrially cultured plectropomus leopardus. The method comprises the following steps: screening healthy plectropomus leopardus individuals from a plectropomus leopardus population as parent fishes; putting the fish in an indoor culture pond for water adaptation to obtain high-quality parent fish; performing induced culture on the high-quality parent fish, wherein the induced culture comprises nutrient enrichment and temperature induction; the temperature induction time is less than or equal to 35 days to promote the ovary maturation of the parent fish. Through an indoor induction mode combining low-temperature energy storage, gradient rewarming and nutrient enrichment feed, the maturation rate of the female plectropomus leopardus ovary can be remarkably increased to 85% or above, and the maturation period is shortened to be within 40 days. The method is suitable for a standardized breeding process of an indoor parent fish breeding workshop, effectively realizes synchronous maturation and quality improvement of the parent fish group ovary, provides a stable parent guarantee for indoor artificial breeding of plectropomus leopardus, and has a good popularization and application prospect.

The invention discloses a traditional Chinese medicine composition for treating or improving hypoovarian reserve function and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: angelica sinensis, parched white paeony root, parasitic loranthus, dodder, roasted rhizoma atractylodis macrocephalae, ligusticum wallichii, herba lycopi, albizia flower and honey-fried licorice root. And after ovulation, morinda officinalis and glossy privet fruit are added. The formula provided by the invention complies with the natural law of human bodies, and according to the growth of qi and blood, yin and yang, nourishing blood, activating blood and tonifying kidney, yin and yang are tonified without stagnation, and blood stasis is removed without hurting healthy qi; meanwhile, the traditional Chinese medicine is used for strengthening the root of acquired days, quieting the main internal organs, combining dynamic and static, regulating qi-blood yin-yang balance and taking the balance as a period. The invention further provides application of the composition or the traditional Chinese medicine extract or the traditional Chinese medicine preparation in preparation of a medicine for treating or improving ovarian reserve hypofunction.

The invention provides application of combination of antrodia camphorata polysaccharide and forsythin in preparation of drugs for treating metabolic diseases related to insulin resistance, and belongs to the technical field of biological medicines. The metabolic diseases related to insulin resistance include polycystic ovarian syndrome. Animal experiments find that the combination of the antrodia camphorata polysaccharide and forsythin can significantly reduce serum testosterone and luteinizing hormone levels of a rat with the polycystic ovarian syndrome, improve serum follicular hormone and estradiol levels, and achieve the effect of improving the polycystic ovarian syndrome.

The present invention discloses the application of MY-1B in the preparation of drugs for improving ovarian senescence, belonging to the technical field of biomedicine, aiming to solve the problem of how to effectively delay ovarian senescence and improve ovarian function. Experiments have proved that during ovarian senescence, the mRNA m<supgt;5< / supgt;C modification level and the expression level of methyltransferase in ovarian granulosa cells increase. Supplementation with MY-1B can improve the condition caused by mRNA m Nsun2 expression.

The invention provides a polypeptide with an ovary maturation promoting effect and application of the polypeptide in parturition induction of pomfret. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide provided by the invention is derived from the GnRH2 gene of the pomfret, and in-vivo experiments prove that the polypeptide can efficiently stimulate the pituitary of the pomfret to secrete gonadotropin, so that the spawning induction time is remarkably shortened, and the spawning rate and the relative spawning amount of parent fish are increased. The polypeptide is strong in species specificity and good in physiological compatibility, does not generate an antibody, avoids side effects of traditional heterologous hormones, is safe and effective, and has a huge potential of being developed into a special spawning induction agent for the pomfret.

Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa; at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof; and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.

The invention discloses a culture device for improving polycystic ovarian syndrome lactobacillus reuteri, and belongs to the technical field of lactobacillus reuteri culture.The culture device comprises a working frame, the inner side of the working frame is fixedly connected with a feeding mechanism used for conveying, one side of the feeding mechanism is fixedly connected with a culture mechanism, and the other side of the feeding mechanism is fixedly connected with a conveying mechanism; a liquid feeding mechanism is fixedly connected to the rear side of the feeding mechanism, a supporting mechanism is fixedly connected to the position, located between the feeding mechanism and the culture mechanism, of the inner side of the working frame, an auxiliary ladder is fixedly connected to the surface of one side of the working frame, and a protection frame is fixedly connected to the surface of the auxiliary ladder. On the basis of realizing auxiliary culture of lactobacillus reuteri, strain functions and metabolites can be accurately regulated and controlled, and the delivery problem in PCOS treatment can be solved.

The invention discloses a ZaSCL3 gene for regulating and controlling plant growth and development as well as an encoding protein and application thereof, and belongs to the technical field of gene engineering. According to the invention, an over-expression vector of the ZaSCL3 gene is constructed and expressed in a wild tomato, so that stamens of a ZaSCL3 over-expression plant are aborted, ovary is spontaneously expanded without pollination, and seedless fruits are generated, which indicates that the over-expression ZaSCL3 gene can realize the effect of inducing male sterility and apomixis of the plant; a key gene resource is provided for molecular breeding and yield character improvement, and the important application value is achieved.

The invention relates to the technical field of medical health, and particularly discloses a polycystic ovarian syndrome typing management system based on big data, the system integrates insulin level and LH / FSH ratio time sequence data of a patient, and adopts zero sequence processing, first order difference and time frequency analysis methods to extract metabolism and endocrine characteristics; and a comprehensive health feature vector is constructed through fusion and is input into a risk prediction model based on Bayesian network training, so that accurate identification of the type of the polycystic ovarian syndrome is realized, further, the system dynamically generates a personalized intervention scheme according to an identification result, and doctor-patient information synchronization and continuous optimization are realized through a cloud platform. By means of the method, the accuracy and scientificity of polycystic ovarian syndrome type typing are improved, individualized and intelligent disease management is achieved, and the method has good clinical application prospects and popularization value.

The invention relates to the technical field of traditional Chinese medicine conditioning, in particular to eight-treasure brewing paste for treating ovarian reserve hypofunction and a preparation method of the eight-treasure brewing paste. The eight-treasure brewing paste is prepared from the following raw materials: prepared rehmannia root, white paeony root, Chinese angelica, ligusticum wallichii, radix pseudostellariae, poria cocos, bighead atractylodes rhizome, liquorice, astragalus membranaceus, parasitic loranthus, bran-fried Chinese yam, salted fructus alpiniae oxyphyllae, cortex albiziae, vinegar-processed rhizoma cyperi, fructus akebiae, donkey-hide gelatin, tortoise-shell glue, fingered citron, wine-processed rhizoma polygonati, caulis spatholobi, mulberry and pericarpium citri reticulatae through pretreatment, water decoction extraction, vacuum concentration, glue melting and mixing and paste collection. The prepared eight-treasure brewing paste has the effects of benefiting qi, nourishing blood, tonifying kidney, soothing liver and regulating Chong and Ren meridians, can improve related symptoms of ovarian reserve hypofunction and regulate the balance of qi, blood, yin and yang of a body, and is scientific in preparation process, sufficient in effective component dissolution, convenient to take and absorb in dosage form and suitable for large-scale popularization and application. And a safe and convenient new choice is provided for the traditional Chinese medicine conditioning of the ovarian reserve hypofunction.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention discloses application of an ACSL4 inhibitor in preparation of a medicine for improving quality reduction of an elderly ovum, and belongs to the technical field of biological medicines. The invention provides an application of targeting long-chain fatty acid acyl-coenzyme A synthetase 4 (ACSL4) in intervention of low oocyte quality and related fertility decline caused by aging. The ACSL4 inhibitor can be used for preparing drugs for improving ovarian functions and improving oocyte quality. In-vivo and in-vitro experiments prove that by inhibiting the ACSL4, the aging process of the elderly ovary can be effectively relieved, the expression of aging-related proteins p16 and p21 can be reduced, the mitochondrial function of the oocyte can be improved, and spindle assembly abnormality and chromosome arrangement defects can be rescued, so that the maturation rate and the development potential of the oocyte can be remarkably improved.

The invention relates to the field of antibacterial gels, in particular to an ovary maintenance antibacterial gel as well as a preparation method and application thereof. The invention relates to an ovary maintenance antibacterial gel, which comprises a component A, a component B and a component C, the component A is prepared from 64 to 84 parts of deionized water, 0.05 to 0.15 part of preservative synergist, 0.6 to 0.9 part of carbomer U-30, 0.06 to 0.12 part of ultra-high molecular weight hyaluronic acid, 3 to 7 parts of hydrogel, 3 to 6 parts of glycerol and 3 to 6 parts of propylene glycol; the component B is prepared from 3 to 7 parts of selective estrogen receptor regulator and 0.5 to 2.5 parts of flos lonicerae extract; the component C is prepared from the following components in parts by weight: 0.1 to 0.3 part of silver ion solution, 0.3 to 0.6 part of preservative, 0.05 to 0.15 part of tween-20 and 0.05 to 0.25 part of triethanolamine. The gel obtained in the invention is applied to ovaries, can play a role in bacteriostasis, and can also effectively improve premature ovarian failure.

The invention discloses a construction method of a zebra fish reproductive toxicity model, a method for evaluating reproductive toxicity of chemical substances and application, and relates to the technical field of reproductive development toxicity of chemical substances. According to the method for evaluating the reproductive toxicity of the chemical substance provided by the invention, the change of a diatrix behavior can be concerned by shooting behavioral pictures or videos without sacrifice of an experimental animal or professional fluorescence imaging instrument equipment and tissue slicing equipment; the method is more in line with animal ethics, cost is saved, and continuous observation can be realized by using the same batch of organisms. The test courtship behavior can quickly utilize software to analyze and obtain zebra fish behavior changes. By adopting the evaluation method provided by the invention, both female ovarian malformation and male reproductive toxicity can be detected, and the evaluation method has the advantage of more evaluation dimensions. Based on the method provided by the invention, a zebrafish reproductive toxicity model can be constructed, and the model can be used as a drug screening platform for reproductive function damage of zebrafish.

The invention discloses a traditional Chinese medicine composition for preventing and treating premature ovarian failure and application thereof. Belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition comprises gordon euryale seed, agilawood, sandalwood, processed rhizoma pinelliae, rheum officinale, amomum cardamomum, alpinia katsumadai, processed rhizoma arisaematis, processed radix aconiti, asarum, fructus aurantii and fructus piperis longi. The composition follows the compatibility principle of monarch, minister, assistant and guide in traditional Chinese medicine, belongs to a composite traditional Chinese medicine preparation, and needs to regulate the reproductive endocrine function through the synergistic effect of multiple medicinal materials. The traditional Chinese medicine focuses on phlegm-dampness stasis and kidney-yang deficiency type infertility and premature ovarian failure. The prior art purely focuses on tonifying the kidney (such as prepared rehmannia root and dogwood) or activating blood (such as peach kernel and safflower), while the formula disclosed by the invention integrates multiple effects of resolving dampness, warming and dredging, removing stasis, promoting the circulation of qi and the like, and is stronger in pertinence.

The invention relates to health-preserving conditioning tea and a preparation method thereof, and belongs to the technical field of tea drinks, and the health-preserving conditioning tea comprises a paederia scandens extract, a radix fici simplicissimae extract, a Chinese yam-eclipta alba distilled liquid medicine and an angelica sinensis extract. The prepared health-preserving conditioning tea can help human body aging. Meanwhile, ovarian senescence can be delayed, and the ovarian health state can be improved.