44 results about "Clostridium" patented technology

The invention discloses a monoclonal antibody of fecal coccus and application of the monoclonal antibody. The monoclonal antibody is a monoclonal antibody CE1 or a monoclonal antibody CE2; the method comprises the following steps: selecting two proteins expressing an S-layer protein and an anchoring protein binding region of coprococcus faecalis, preparing two specific antibodies CE1 and CE2 for identifying the coprococcus faecalis by using the two proteins, respectively coupling the two antibodies to magnetic beads, and enriching the coprococcus faecalis by using the two magnetic bead-antibody conjugates to obtain the coprococcus faecalis magnetic bead-antibody conjugate. The principle that CE1 and CE2 antibodies are combined with specific antigens on the surfaces of bacteria is utilized, so that the regular faecalis coccus can be specifically enriched.

The invention discloses a traditional Chinese medicine composition and application thereof in medicine for improving non-alcoholic fatty liver, and the traditional Chinese medicine composition comprises pulvis fellis suis, pericarpium citri reticulatae, hawthorn and clostridium butyricum. On one hand, potential targets and pathways of drug diseases are determined in network pharmacological analysis, and on the other hand, in an in-vivo experiment, the drug provided by the invention improves obesity and impaired glucose tolerance caused by HFD, reduces the blood fat level, alleviates inflammation, protects the liver, alleviates liver fat vacuole deposition and fatty degeneration, and has a good application prospect. It is proved that the bile acid-hawthorn and pericarpium citri reticulatae-clostridium butyricum combination has prevention and treatment and protection effects on the HFD-induced non-alcoholic fatty liver disease.

The invention belongs to the technical field of biological enzymes, and particularly relates to mutant clostridium sporogenes source phenyllactic dehydrogenase, a method for preparing indole-3-lactic acid from the mutant clostridium sporogenes source phenyllactic dehydrogenase and application of the mutant clostridium sporogenes source phenyllactic The mutant type clostridium sporogenes source phenyllactic acid dehydrogenase comprises N53V mutant type clostridium sporogenes source phenyllactic acid dehydrogenase, R154K mutant type clostridium sporogenes source phenyllactic acid dehydrogenase and N53V / R154K mutant type clostridium sporogenes source phenyllactic acid dehydrogenase. The amino acid sequences of the polypeptide are respectively shown as SEQ ID NO: 6, SEQ ID NO: 10 and SEQ ID NO: 12. Constructing a genetically engineered bacterium for expressing the mutant phenyllactic dehydrogenase in escherichia coli; and tryptophan can be efficiently fermented and catalyzed to be converted into indole-3-lactic acid, and the yield of the indole-3-lactic acid generated by fermentation is increased to 1.85 times, so that the mutant has a very good application prospect in the aspect of fermentation preparation of the indole-3-lactic acid.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

The invention discloses a 5 / 8 / 5 chitosan clostridium diterpenoid compound, a biosynthetic gene cluster and application of the biosynthetic gene cluster, and relates to the technical field of biological medicines. A 5 / 8 / 5 chitin type diterpenoid biosynthetic gene cluster Thm in trichoderma harzianum is identified through a genome mining system, chitin diterpenoid compounds with diversified structures are obtained by means of heterologous expression, the obtained compounds are subjected to anti-thrombotic activity comprehensive evaluation, and the result shows that the compounds 6 and 31 can obviously inhibit vascular thrombosis, the compound 6 has a remarkable inhibition effect on collagen-induced platelet aggregation, and the compound 31 has a better inhibition capability on thrombin-induced platelet activation. The compounds 6 and 31 show powerful antithrombotic effects in artery, vein and pulmonary artery thrombosis models, so that powerful technical support is provided for development of antithrombotic drugs.

Interleukin-23 receptor (IL23R) VHH antibodies are disclosed, as well as methods of making and using the same, e.g., for treatment of IL23-mediated diseases and disorders, including, e.g., gastrointestinal-related (Gl-related) diseases (e.g., inflammatory bowel disease (IBD) (e.g., ulcerative colitis (UC) or Crohn's disease (CD)), a colon cancer, a small intestine cancer, a gastric cancer, an irritable bowel syndrome, a gastrointestinal ulcer, a gut-associated infection (e.g., a Salmonella infection or a Clostridium difficile infection), celiac disease, or pathogenic inflammation).

The present application relates to the technical field of animal breeding, and discloses a compound nutritional health supplement for laying hens in the egg production period and a preparation and application thereof, which comprises the following raw materials in mass fractions: motherwort 1200-1400 parts, radix astragali 150-250 parts, radix codonopsis 80-120 parts, betaine hydrochloride 500-700 parts, butyric acid clostridium 50-70 parts, bacillus licheniformis 90-110 parts, vitamin acetate 3-5 parts, coated 25-hydroxy-cholecalciferol 1-2 parts, vitamin E acetate 15-25 parts, and rice husk powder 0-411 parts. The formula can scientifically and comprehensively regulate the digestion and absorption of nutrients, immune function and reproductive system health of laying hens, improve the eggshell quality, egg weight and nutritional quality of egg products, has stable effect and strong universality, greatly reduces the side effects and body burden caused by long-term medication while ensuring the effect, and is more suitable for the concept of healthy breeding; the health level of the body is comprehensively improved, the intestinal flora balance is maintained by probiotics, the immunity and stress resistance of laying hens are overall enhanced, the nutrient utilization rate is improved, and healthy high yield is achieved.

This invention discloses the application of a morel mycelium extract, belonging to the field of natural products. The morel mycelium extract is mainly composed of α-glucan, with a weight-average molecular weight of 10,000 kDa to 15,000 kDa, a number-average molecular weight of 10,000 kDa to 12,000 kDa, a polydispersity index of 1.0-1.3, and a glucose molar percentage of not less than 99%. The preparation method includes ultrasonic-assisted extraction, alcohol precipitation, enzymatic hydrolysis, dialysis, and ethanol gradient purification. The morel mycelium extract of this invention has the effect of regulating intestinal flora, specifically by improving… Enterococcus , Citrobacter and Bacillus Species abundance of genera, decreased Clostridium and Ruminococcus It has a high species abundance and is more easily utilized by the human gut microbiota than inulin, rapidly decomposing to produce short-chain fatty acids.

This invention relates to the field of biotechnology, specifically to compositions and their application in the preparation of drugs for the prevention and treatment of lymphoma. The invention provides a composition comprising clostridium tumefaciensin and epipitorone, which synergistically enhance the effects of targeting the LCP2-mediated IQGAP2 / LaminA / C / SUV39H1 axis to combat chemotherapy resistance in NK / T cell lymphoma. The combined use of these two drugs can significantly reverse chemotherapy resistance, providing a new treatment strategy and drug combination for relapsed / refractory NKTCL. Experimental comparisons of the efficacy of immunotherapy drugs such as PD-1 inhibitors revealed that the anti-cellular senescence drug combination of clostridium tumefaciensin and epipitorone provided by this invention is significantly more effective than PD-1 inhibitors.

The invention discloses application of an efficient organic material decomposition agent in organic fertilizer fermentation, and belongs to the technical field of organic waste resource utilization. According to the decomposition agent, in-situ microorganisms of an organic fertilizer fermentation system serve as an adaptation basis, and the functional strains are composed of liquid lactobacillus, lactobacillus, acetobacter aceti and clostridium. The thallus concentration of the liquid lactobacillus is 5.0 * 10 < 8 >-1.0 * 10 < 9 > cfu / mL, the thallus concentration of the lactobacillus is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL, the thallus concentration of the acetobacter aceti is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL, and the thallus concentration of the clostridium is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL. The application process of the decomposition agent in organic fertilizer fermentation is optimized, rapid decomposition of organic materials is achieved through the synergistic metabolism effect of functional strains, and the fermentation period is remarkably shortened to 14 days. The application method is easy and convenient to operate, high in adaptability and suitable for organic fertilizer fermentation production of various organic materials such as livestock and poultry manure, straw and wood chips, and has extremely high industrial application value.

It was found that bacteria belonging to the genus Clostridium induce accumulation of regulatory T cells (Treg cells) in the colon. Moreover, the present inventors found that regulatory T cells (Treg cells) induced by from these bacteria suppressed proliferation of effector T-cells. From these findings, the present inventors found that the use of bacteria belonging to the genus Clostridium or a physiologically active substance derived therefrom made it possible to induce proliferation or accumulation of regulatory T cells (Treg cells), and further to suppress immune functions.

This invention provides Bacillus subtilis ( Bacillus subtle The application of strain A45 in the control of plant diseases, its application in the preparation of microbial agents for the control of plant diseases, and the prepared microbial agents. Bacillus subtilis A45 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33289. The liquid microbial agent containing Bacillus subtilis A45 provided by this invention effectively inhibits the cucumber target spot pathogen, *Clostridium multiflorum* (…). Corynespora cassicola The growth mechanism of this strain lies in its encoding a gene cluster for the synthesis of phytosalicylic acid, purchemin, subtilosin A, and surfactant. Among these, the surfactant synthesis gene cluster is unique to this strain, exhibiting a maximum sequence similarity of 82%. Experiments show that the liquid inoculant containing Bacillus subtilis A45 provided by this invention can effectively inhibit the growth of *Clostridium multiflorum*, the pathogen causing target spot disease in cucumbers. Corynespora cashew nut The growth of ) can be controlled by field application with a prevention and control effect of up to 80%.

The invention discloses a compound enzyme bacterium fermented agricultural and sideline product feed and a production process thereof, and relates to the technical field of biological fermentation, the compound enzyme bacterium fermented agricultural and sideline product feed is obtained by anaerobic fermentation of compound enzyme bacterium fermented agricultural and sideline products; the agricultural and sideline products are cotton straws, corn flour, cottonseed hulls, cottonseed meal and bran; the compound enzyme bacteria are obtained by mixing soft rot fungi, trichoderma, cellulase, clostridium, manganese peroxidase and lactic acid bacteria; according to the compound enzyme bacterium fermented agricultural and sideline product feed disclosed by the invention, the cotton byproducts are treated by adopting a microbial fermentation method, after microbial fermentation, straw lignin, hemicellulose, gossypol, crude fibers and other components, which are not suitable for being used as feed, in the cotton byproducts are degraded, and the anti-nutritional factor level in the feed is remarkably reduced; the nutritional value of the feed is greatly improved; the feed can be used as a total mixed ration, can be used for raising livestock such as cattle and sheep, greatly reduces the feeding cost, and solves the problem of low utilization rate of cotton by-products.

The invention discloses clove dreg polysaccharide with immune regulation and intestinal flora regulation functions and a preparation method thereof. The preparation method comprises the steps that dried medicine residues obtained after clove is subjected to oil extraction are taken and smashed, then water extraction, concentration, alcohol precipitation, deproteinization and other steps are carried out to obtain clove medicine residue polysaccharide, the novel clove heteropolysaccharide is further obtained through anion exchange chromatography purification, and the heteropolysaccharide is I-type rhamnogalacturonic acid pectic polysaccharide, is composed of eight monosaccharides and has specific structural characteristics. Experiments prove that the polysaccharide prepared by the invention can obviously improve the levels of serum IL-2, IL-6, IFN-gamma, IgG, IgA and IgM, enhance the immunity of the organism, regulate the diversity of intestinal flora, promote the proliferation of beneficial bacteria such as Brautella and clostridium, and inhibit harmful bacteria such as tremospirillum. According to the invention, the resource utilization of the clove dregs is realized, and a new way is provided for developing immunoregulation and intestinal health products.

The invention belongs to the field of biological medicines, and particularly relates to application of Clostridium baumannii in preparation of a product for treating and / or preventing inflammatory bowel diseases.

The present invention relates to the use of one or more ratios of the amount of bacteria in the microbiota of a farm animal as a marker to determine the status of the intestinal microbiota of a farm animal and whether this status is satisfactory, or to the use of an extract for selecting a satisfactory intestinal microbiota of a farm animal, intended to preserve or improve the gut microbiota of different farm animals in need thereof, said ratio being selected from the group consisting of Phylum Thallungiae / Phylum Proteinae; clostridium IV and XlVa cluster / Enterobacteriaceae; a ruminaceae family and a hispiraceae / enterobacteriaceae family; butyrate-producing bacteria / phylum of Thalictrum; aerobic bacteria / anaerobic bacteria.

The invention discloses a medicinal and edible traditional Chinese medicine composition for preventing and controlling necrotic enteritis of poultry. The traditional Chinese medicine composition is an extraction process for optimizing an acacia flower extract (taking total flavonoids as a core index), a fructus chebulae extract (taking gallic acid as a core index) and a honeysuckle flower extract (taking chlorogenic acid as a core index), aims at extraction efficiency and active ingredient retention rate, and combines a single factor test and an orthogonal test; the system investigates the influence of the water adding times, the decoction time and the decoction times on the extraction effect. Results show that the traditional Chinese medicine composition with homology of medicine and food composed of the robinia flower extract, the fructus chebulae extract and the honeysuckle flower extract can effectively prevent and control necrotic enteritis of poultry (especially chicken) caused by clostridium perfringens; and the process is stable and feasible and has a good prevention and control effect.

The invention discloses a preparation method of a ficus microcarpa extract and application of the ficus microcarpa extract in intestinal flora disorder caused by circadian rhythm disorder. Pharmacological experiments prove that the ficus striata flavone has a remarkable regulating effect in a circadian rhythm disorder injury model: the ficus striata flavone can be used for effectively solving the problems that the diversity of intestinal flora is reduced, the excessive propagation of firmictures, the expression of Bacteroidetes is reduced and the like, which are caused by circadian rhythm disorder, and can be used for preventing and treating the diseases caused by circadian rhythm disorder, so that the ficus striata flavone can be used for preventing and treating the diseases caused by circadian rhythm disorder. Meanwhile, circadian rhythm oscillation of key bacteria such as prevotella, Bacteroides and Clostridium can be adjusted, and increase of the F / B ratio is reduced, so that the rhythm and stability of intestinal flora are improved. The invention simultaneously discloses two preparation processes, namely an ethanol reflux-resin purification method and a water extraction-concentration-alcohol precipitation method, so that the stable quality of the extract is ensured, and the industrial feasibility is realized.

The present disclosure is related to ex vivo methods and devices for identifying a presence of or predicting a risk for a condition in a subject, wherein the risk or condition is correlated with an oxidation-redox potential (ORP) of a biological sample obtained from the subject, such as a fecal sample. The methods and devices of the present disclosure can be used to diagnose and monitor gut microbiome health and assess the risk and / or presence of conditions such as obesity, metabolic dysfunction associated steatotic liver disease (MASLD), type 2 diabetes, hyperlipidemia, hypertension, cardiovascular disease, a gut specific condition, irritable bowel syndrome, an inflammatory bowel disease, ulcerative colitis, Crohn's disease, a Clostridium difficile infection, or any combination thereof based on an ORP of the subject's sample.

The present application belongs to the technical field of kitchen waste resource, and particularly relates to a modified Enteromorpha hydrothermal carbon and application thereof in promoting enrichment of Clostridium and / or Methanosaeta in a dry digestion system of kitchen waste. The present application optimizes the microbial community structure of the digestion system, enhances the microbial activity, and enriches functional microorganisms such as Clostridium and Methanosaeta by adding an appropriate amount of phosphate-activated magnetic Enteromorpha hydrothermal carbon in the dry anaerobic digestion system of kitchen waste, thereby effectively promoting the dry anaerobic digestion process of kitchen waste and improving the biogas production. The present application has great significance for realizing kitchen waste reduction, harmlessness and resource.

The application discloses a kind of colorectal cancer plasma circulating microbial markers, reagent, kit and its application, belong to biomedical detection technical field.The colorectal cancer plasma circulating microbial markers of the present application include alternaria alternata, xanthomonas campestris, clostridium perfringens, human rumblus, pseudomonas syringae, alternaria alternata, parabacillus licheniformis and pseudomonas szechuanensis.The colorectal cancer plasma circulating microbial markers of the present application can be used for early screening of colorectal cancer, with high sensitivity and specificity, significantly improve the accuracy and reliability of detection;Break through the limitation of traditional ctDNA detection, improve the detection rate of early colorectal cancer.Through low-depth whole genome sequencing, the colorectal cancer plasma circulating microbial markers in colorectal cancer plasma sample are detected, the requirement for depth in experiment is reduced, while ensuring high sensitivity and specificity, cost can be greatly reduced, more suitable for large-scale screening.

[Problem] The purpose of the present invention is to provide: a composition capable of promoting the secretion of serotonin; food or drink, a cosmetic, and a medicine containing the composition; and methods for producing the same. [Solution] Provided is a serotonin secretion-promoting composition containing at least two types of enteric bacteria. In particular, the enteric bacteria are preferably selected from enteric bacteria belonging to the genus Eubacterium, Clostridium, or Bifidobacterium.

The invention discloses a Clostridium strain with a prevention or treatment effect on neurodegenerative brain diseases and an application of the Clostridium strain. The Clostridium strain provided by the invention has a neuroprotective effect, has a remarkable improvement effect in animal models of degenerative brain diseases such as Parkinson's disease and Alzheimer's disease, and has the effects of relieving dyskinesia and non-dyskinesia in the animal models of Parkinson's disease, including cognitive impairment, gastrointestinal dysfunction and the like; therefore, the strain can be effectively applied to foods and medicines for preventing or treating neurodegenerative brain diseases including Parkinson's disease, Alzheimer's disease, cognitive function impairment and the like.

Provided are a method for enhancing the immunogenicity of a recombinant protein antigen and use thereof. The method comprises conjugating a polysaccharide with a recombinant protein antigen to form a nano-scale protein antigen. The polysaccharide is selected from sodium hyaluronate, chitosan, glucan, fucoidan, and sodium alginate. The recombinant protein antigen is a protein antigen derived from a bacterium. The bacterium belongs to the genus of Staphylococcus, Neisseria, Klebsiella, Escherichia, Clostridium, Salmonella, Shigella, Pseudomonas, Acinetobacter, Bordetella, Enterococcus, Haemophilus, Mycobacterium, or Streptococcus. The method not only improves the immunogenicity of the recombinant protein antigen, but also overcomes the defects of competitive immunoregulation and poor immune enhancement effects caused by bacterial capsular polysaccharide modification. The method also has the advantages of high clinical application value, simple starting material acquisition, low cost, good process stability, ease in scale expansion, high safety, and the like.

The present application belongs to the technical field of kitchen waste resource, and particularly relates to a kind of polyaniline magnetic biochar prepared based on biogas residue and its application in promoting anaerobic co-digestion system anaerobic co-digestion synergistic function bacteria and methanogenic archaea enrichment. The present application optimizes the microbial community structure of the dry anaerobic digestion system of kitchen waste by adding an appropriate amount of phosphate activated magnetic Enteromorpha hydrothermal carbon to enhance microbial activity, enrich functional microorganisms such as Clostridium and Methanocella, effectively promote the dry anaerobic digestion process of kitchen waste, and increase the amount of biogas production. It has great significance for realizing kitchen waste reduction, harmless and resource.

The application belongs to the technical field of biomedical diagnosis, and provides an intestinal flora marker combination for retinopathy of premature infants and application thereof, wherein the intestinal flora marker combination comprises Klebsiella aerogenes and / or Clostridium butyricum as detection targets. The application first identifies specific intestinal flora markers related to the risk of retinopathy of premature infants at the species level. The abundance of Klebsiella aerogenes in the intestinal flora is positively correlated with the risk of retinopathy of premature infants, and can be used as a risk warning marker for retinopathy of premature infants. The abundance of Clostridium butyricum in the intestinal flora is negatively correlated with the risk of retinopathy of premature infants, and can be used as a protective marker for retinopathy of premature infants. The application provides a new non-invasive detection target for early warning, risk assessment and prognosis of retinopathy of premature infants.

The present disclosure is related to ex vivo methods and devices for identifying a presence of or predicting a risk for a condition in a subject, wherein the risk or condition is correlated with an oxidation-redox potential (ORP) of a biological sample obtained from the subject, such as a fecal sample. The methods and devices of the present disclosure can be used to diagnose and monitor gut microbiome health and assess the risk and / or presence of conditions such as obesity, metabolic dysfunction associated steatotic liver disease (MASLD), type 2 diabetes, hyperlipidemia, hypertension, cardiovascular disease, a gut specific condition, irritable bowel syndrome, an inflammatory bowel disease, ulcerative colitis, Crohn's disease, a Clostridium difficile infection, or any combination thereof based on an ORP of the subject's sample.

The invention belongs to the technical field of plant extracting solutions, and particularly relates to a roselle extracting solution for optimizing intestinal flora and a preparation method of the roselle extracting solution. The roselle calyx is stewed with the rice meal, gamma-aminobutyric acid or glutathione in the rice meal can permeate into the calyx, the effect of the calyx is enhanced, and the roselle calyx is more suitable for being absorbed and utilized by the human body subsequently; the simmered calyx is subjected to ultrasonic extraction, so that effective components of the calyx can be sufficiently separated out; in addition, the calyx filtrate and the leaf filtrate are compounded according to a specific proportion and have a synergistic effect, so that the effect of the roselle extracting solution is improved. Therefore, the hibiscus sabdariffa extracting solution provided by the invention can promote the increase of the number of lactobacillus in the intestinal tract and inhibit the reproduction of enterobacter and clostridium perfringens in the intestinal tract, so that the hibiscus sabdariffa extracting solution can be used for optimizing intestinal flora and preparing corresponding products.

The invention provides a temperature response type intelligent slow-release carbon source, a preparation method thereof and application of the temperature response type intelligent slow-release carbon source in sewage treatment, and belongs to the technical field of sewage treatment. According to the temperature-responsive intelligent slow-release carbon source disclosed by the invention, temperature-sensitive hydrogel is taken as a skeleton, and the skeleton of the temperature-sensitive hydrogel is formed by copolymerization and cross-linking reaction of N-vinyl caprolactam, acrylamide and carboxymethyl cellulose; a slow-release carbon source material is embedded in the temperature-sensitive hydrogel skeleton, and the slow-release carbon source material comprises corn bran and polyhydroxyalkanoate. The temperature response type intelligent slow-release carbon source obtained by the invention can effectively promote efficient removal of nitrate, can significantly enrich key denitrifying bacteria such as Hydrogenphaga, Clostridium and the like, and enhances the network modularity of microbial communities and the positive correlation between species. The invention provides a theoretical and technical basis for developing an intelligent carbon source for self-adaptive tail water treatment.