91 results about "Clostridium" patented technology

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.

Provided are methods, prokaryotic host cells, expression vectors, and kits for the production of psilocybin derivatives produced using at least one psilocybin production gene. The methods enable production of psilocybin derivatives and related compounds including non-naturally occurring species. In certain embodiments, a prokaryotic host cell is selected from the group consisting of from the group consisting of Escherichia coli, Corynebacterium glutamicum, Vibrio natriegens, Bacillus subtilis, Bacillus megaterium, Escherichia coli Nissle 1917, Clostridium acetobutlyicum, Streptomyces coelicolor, Lactococcus lactis, Pseudomonas putida, Streptomyces clavuligerus, and Streptomyces venezuelae.

Provided herein are compositions comprising bacterial mixtures of strains from genera including Roseburia, Dorea, Bacteroides, Coprobacter, Agathobaculum, Lawsonibacter, Tyzzerella, Phascolarctobacterium, Enorma, Faecalimonas, lllomasclavelia, Clostridium, Fournierella, and / or Massilimicrobiota. Also provided are methods of using compositions comprising such bacterial mixtures. In some embodiments, it includes compositions, kits, and methods that are useful, e.g., for treatment and / or prevention of inflammatory bowel disease (IBD), such as ulcerative colitis (UC).

The invention belongs to the technical field of medicine, and particularly relates to a medicine for treating or preventing chicken coccidiosis and chicken necrotic enteritis and application, the medicine is chaulmoogratree seed, and can be in the form of medicinal material powder or chaulmoogratree seed extract. The traditional Chinese medicine composition can be used for preventing and treating chicken coccidiosis diseases, reducing the death rate of broilers with necrotic enteritis and intestinal lesions of necrotic enteritis, effectively improving chicken necrotic enteritis and has a good popularization and application prospect in the aspect of preventing and treating chicken necrotic enteritis diseases; the traditional Chinese medicine composition has a good treatment effect on mixed infection diseases of small intestine coccidiosis and clostridium.

A mixture includes a nickel-containing ore and / or concentrate thereof and an extraction composition including a fermentation broth obtained from an Aspergillus species (spp.), Acetobacter spp., Bacillus spp., Propionibacterium spp., Corynebacterium spp., Rhizopus spp., Clostridium spp., Fusobacterium spp., Pseudomonas spp., Bifidobacterium spp., Saccharomyces spp., Enterobacter spp., Escherichia spp., among others. The extraction composition is configured to selectively solubilize one or more components, such as a magnesium component from the nickel-containing ore and / or concentrate thereof. A method for extracting a metal from a nickel-containing ore and / or concentrate includes selectively solubilizing a magnesium component from the nickel-containing ore and / or concentrate thereof into an extraction composition including the fermentation broth. The method can include extracting a magnesium component from a nickel-containing ore and / or concentrate thereof into an extraction composition, separating the nickel-containing ore and / or concentrate thereof from an extracted magnesium component and broth, separating the broth from the extracted magnesium component, and repeating the extracting. The extracted magnesium component has a purity in a range from 10% to 90%.

The present invention discloses a method containing VB 12 The production method of the medicinal plant additive for suckling pig feed and the product thereof relate to the field of suckling pig breeding. The production method of the medicinal plant additive for suckling pig feed of the present invention comprises the following steps: (1) crushing cardamom, tricholoma, oregano and inulin respectively and enzymolyzing them with cellulase, and then mixing them in proportion, or first mixing cardamom, tricholoma, oregano and inulin in proportion, and then crushing and enzymolyzing them with cellulase to obtain a primary enzymolysis material; (2) adding potassium dihydrogen phosphate and oxalic acid to the primary enzymolysis material, and continuing to degrade to obtain a hot-cooked enzymolysis material; (3) mixing the hot-cooked enzymolysis material with molasses, wormwood, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material; (4) adding pretreated Sanguisorba officinalis and molasses to the primary fermentation material, and continuing to ferment. The medicinal plant additive for suckling pig feed of the present invention can significantly reduce the feed-to-meat ratio of suckling pigs and significantly increase daily weight gain.

The invention discloses a monoclonal antibody of fecal coccus and application of the monoclonal antibody. The monoclonal antibody is a monoclonal antibody CE1 or a monoclonal antibody CE2; the method comprises the following steps: selecting two proteins expressing an S-layer protein and an anchoring protein binding region of coprococcus faecalis, preparing two specific antibodies CE1 and CE2 for identifying the coprococcus faecalis by using the two proteins, respectively coupling the two antibodies to magnetic beads, and enriching the coprococcus faecalis by using the two magnetic bead-antibody conjugates to obtain the coprococcus faecalis magnetic bead-antibody conjugate. The principle that CE1 and CE2 antibodies are combined with specific antigens on the surfaces of bacteria is utilized, so that the regular faecalis coccus can be specifically enriched.

The invention belongs to the technical field of kitchen waste recycling, and particularly relates to polyaniline magnetic biochar prepared on the basis of biogas residues and application of the polyaniline magnetic biochar in promoting enrichment of anaerobic co-digestion synergistic functional bacteria and methanogenic archaea in an anaerobic co-digestion system. According to the method, a proper amount of phosphate is added into the dry anaerobic digestion system of the kitchen garbage to activate the magnetic enteromorpha hydrothermal carbon so as to optimize the microbial community structure of the digestion system, enhance the microbial activity and enrich functional microorganisms such as clostridium and methane bag-shaped bacteria, so that the dry anaerobic digestion process of the kitchen garbage is effectively promoted, and the digestion efficiency of the kitchen garbage is improved. And the biogas yield is improved. And the method has great significance for realizing reduction, harmlessness and recycling of the kitchen garbage.

The invention discloses a traditional Chinese medicine composition and application thereof in medicine for improving non-alcoholic fatty liver, and the traditional Chinese medicine composition comprises pulvis fellis suis, pericarpium citri reticulatae, hawthorn and clostridium butyricum. On one hand, potential targets and pathways of drug diseases are determined in network pharmacological analysis, and on the other hand, in an in-vivo experiment, the drug provided by the invention improves obesity and impaired glucose tolerance caused by HFD, reduces the blood fat level, alleviates inflammation, protects the liver, alleviates liver fat vacuole deposition and fatty degeneration, and has a good application prospect. It is proved that the bile acid-hawthorn and pericarpium citri reticulatae-clostridium butyricum combination has prevention and treatment and protection effects on the HFD-induced non-alcoholic fatty liver disease.

The invention belongs to the technical field of biological enzymes, and particularly relates to mutant clostridium sporogenes source phenyllactic dehydrogenase, a method for preparing indole-3-lactic acid from the mutant clostridium sporogenes source phenyllactic dehydrogenase and application of the mutant clostridium sporogenes source phenyllactic The mutant type clostridium sporogenes source phenyllactic acid dehydrogenase comprises N53V mutant type clostridium sporogenes source phenyllactic acid dehydrogenase, R154K mutant type clostridium sporogenes source phenyllactic acid dehydrogenase and N53V / R154K mutant type clostridium sporogenes source phenyllactic acid dehydrogenase. The amino acid sequences of the polypeptide are respectively shown as SEQ ID NO: 6, SEQ ID NO: 10 and SEQ ID NO: 12. Constructing a genetically engineered bacterium for expressing the mutant phenyllactic dehydrogenase in escherichia coli; and tryptophan can be efficiently fermented and catalyzed to be converted into indole-3-lactic acid, and the yield of the indole-3-lactic acid generated by fermentation is increased to 1.85 times, so that the mutant has a very good application prospect in the aspect of fermentation preparation of the indole-3-lactic acid.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

The invention belongs to the technical field of aquaculture, and discloses application of chitosan oligosaccharide in preparation of fish feed for promoting fish growth and improving disease resistance of grass carp bodies. Chitosan oligosaccharide is found to be capable of promoting fish growth and improving the disease resistance of grass carp bodies. In the embodiment, chitosan oligosaccharide is added as an immunopotentiator, so that non-specific antioxidant enzyme expression of the liver of the grass carp can be effectively induced, and the autoimmunity of the grass carp is improved. The chitosan oligosaccharide can improve the intestinal flora structure of the grass carp. In the embodiment, the relative abundance of the clostridium of the 3% chitosan oligosaccharide addition group is remarkably higher than that of the control group, and the 3% chitosan oligosaccharide addition group is the most important bacterial genus of the clostridium, so that the chitosan oligosaccharide has a remarkable effect on improving the intestinal flora structure of the grass carp.

The invention discloses a 5 / 8 / 5 chitosan clostridium diterpenoid compound, a biosynthetic gene cluster and application of the biosynthetic gene cluster, and relates to the technical field of biological medicines. A 5 / 8 / 5 chitin type diterpenoid biosynthetic gene cluster Thm in trichoderma harzianum is identified through a genome mining system, chitin diterpenoid compounds with diversified structures are obtained by means of heterologous expression, the obtained compounds are subjected to anti-thrombotic activity comprehensive evaluation, and the result shows that the compounds 6 and 31 can obviously inhibit vascular thrombosis, the compound 6 has a remarkable inhibition effect on collagen-induced platelet aggregation, and the compound 31 has a better inhibition capability on thrombin-induced platelet activation. The compounds 6 and 31 show powerful antithrombotic effects in artery, vein and pulmonary artery thrombosis models, so that powerful technical support is provided for development of antithrombotic drugs.

The invention belongs to the technical field of organic substrates, and particularly relates to an organic substrate preparation process based on complex microbial inoculant synergistic fermentation, which comprises the following steps: selecting agricultural wastes, livestock and poultry manure and food industry byproducts, and carrying out staged fermentation by adopting complex microorganisms such as bacillus, streptomyces, clostridium and bacteroides; the decomposition efficiency of degradation-resistant substances such as cellulose and lignin is obviously improved. By combining precise temperature control and oxygen concentration adjustment strategies, the stability of the fermentation process is enhanced, nitrogen volatilization is reduced, and the total nitrogen content is stabilized at 1.2-1.5%. Nitrogen-fixing bacteria and phosphate solubilizing bacteria are introduced in the after-ripening stage, the biological activity of the matrix is improved, the screening process ensures that the finished product is uniform in particle size and few in impurities, the content of organic matter reaches 45% or above, the pH value is stabilized at 6.5-7.5, excellent agricultural performance is achieved, and efficient resource utilization of organic waste is achieved; the problems that in the prior art, organic matter degradation is not thorough, the controllability of the fermentation process is poor, nutrient loss is serious, and the quality of a finished product is unstable are effectively solved.

Interleukin-23 receptor (IL23R) VHH antibodies are disclosed, as well as methods of making and using the same, e.g., for treatment of IL23-mediated diseases and disorders, including, e.g., gastrointestinal-related (Gl-related) diseases (e.g., inflammatory bowel disease (IBD) (e.g., ulcerative colitis (UC) or Crohn's disease (CD)), a colon cancer, a small intestine cancer, a gastric cancer, an irritable bowel syndrome, a gastrointestinal ulcer, a gut-associated infection (e.g., a Salmonella infection or a Clostridium difficile infection), celiac disease, or pathogenic inflammation).

The present application relates to the technical field of animal breeding, and discloses a compound nutritional health supplement for laying hens in the egg production period and a preparation and application thereof, which comprises the following raw materials in mass fractions: motherwort 1200-1400 parts, radix astragali 150-250 parts, radix codonopsis 80-120 parts, betaine hydrochloride 500-700 parts, butyric acid clostridium 50-70 parts, bacillus licheniformis 90-110 parts, vitamin acetate 3-5 parts, coated 25-hydroxy-cholecalciferol 1-2 parts, vitamin E acetate 15-25 parts, and rice husk powder 0-411 parts. The formula can scientifically and comprehensively regulate the digestion and absorption of nutrients, immune function and reproductive system health of laying hens, improve the eggshell quality, egg weight and nutritional quality of egg products, has stable effect and strong universality, greatly reduces the side effects and body burden caused by long-term medication while ensuring the effect, and is more suitable for the concept of healthy breeding; the health level of the body is comprehensively improved, the intestinal flora balance is maintained by probiotics, the immunity and stress resistance of laying hens are overall enhanced, the nutrient utilization rate is improved, and healthy high yield is achieved.

The present application relates to a kind of compound preparation for preventing and treating poultry enteritis diarrhea, it is mainly made of the following weight parts proportion raw materials: catnip 10-12 parts, 10-12 parts of banlangen, 8-10 parts of heartleaf hedyotis, 10-12 parts of pepper, 10-15 parts of green wormwood, 8-10 parts of Chinese clematis, 10-12 parts of atractylodes, 3-5 parts of xylo-oligosaccharide, 1-3 parts of garlicin, 1-3 parts of capsaicin, 5-7 parts of vitamin C, 0.1-0.3 parts of vitamin A, 4-5 parts of bacillus licheniformis, 4-5 parts of butyric acid clostridium, 10-12 parts of brown sugar.The compound preparation can effectively improve the immune function of organism, prevent and treat diarrhea caused by poultry enteritis, improve intestinal health;While preparation enters organism, is easily absorbed, and clinical administration is convenient, suitable for group administration, save manpower and material resources.

The present application relates to the technical field of feed additives, and specifically discloses a composite microbial feed additive and a preparation method thereof. The composite microbial feed additive comprises the following components in parts by weight: 10-30 parts of sodium butyrate; 30-50 parts of silicon dioxide; 40-60 parts of bran; 60-80 parts of modified potato starch; 7-15 parts of Clostridium butyricum; 4-9 parts of Lactobacillus plantarum; and 2-6 parts of Bacillus licheniformis. The composite microbial feed additive provided in the present application can effectively increase the daily feed intake and daily weight gain of broilers, reduce the feed-to-weight ratio, and reduce the mortality rate of broilers.

This invention discloses the application of a morel mycelium extract, belonging to the field of natural products. The morel mycelium extract is mainly composed of α-glucan, with a weight-average molecular weight of 10,000 kDa to 15,000 kDa, a number-average molecular weight of 10,000 kDa to 12,000 kDa, a polydispersity index of 1.0-1.3, and a glucose molar percentage of not less than 99%. The preparation method includes ultrasonic-assisted extraction, alcohol precipitation, enzymatic hydrolysis, dialysis, and ethanol gradient purification. The morel mycelium extract of this invention has the effect of regulating intestinal flora, specifically by improving… Enterococcus , Citrobacter and Bacillus Species abundance of genera, decreased Clostridium and Ruminococcus It has a high species abundance and is more easily utilized by the human gut microbiota than inulin, rapidly decomposing to produce short-chain fatty acids.

This invention relates to the field of biotechnology, specifically to compositions and their application in the preparation of drugs for the prevention and treatment of lymphoma. The invention provides a composition comprising clostridium tumefaciensin and epipitorone, which synergistically enhance the effects of targeting the LCP2-mediated IQGAP2 / LaminA / C / SUV39H1 axis to combat chemotherapy resistance in NK / T cell lymphoma. The combined use of these two drugs can significantly reverse chemotherapy resistance, providing a new treatment strategy and drug combination for relapsed / refractory NKTCL. Experimental comparisons of the efficacy of immunotherapy drugs such as PD-1 inhibitors revealed that the anti-cellular senescence drug combination of clostridium tumefaciensin and epipitorone provided by this invention is significantly more effective than PD-1 inhibitors.

The invention relates to direct-fed microbials for use in E. coli, Salmonella, and / or Clostridium inhibition in animals. More particularly, the invention relates to isolated Bacillus strains CM256 (NRRL No. B-67706), CM857 (NRRL No. B-67705), CM978 (NRRL No. B-67703), CM1004 (NRRL No. B-67708), and MDG1607 (NRRL No. B-67666), and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. The invention also relates to the use of isolated Bacillus strains CM256 (NRRL No. B-67706), CM857 (NRRL No. B-67705), CM978 (NRRL No. B-67703), and CM1004 (NRRL No. B-67708), and strains having all of the identifying characteristics of these strains, to treat plants having diseases caused by E. coli, Salmonella, and / or Clostridium.

The invention discloses application of an efficient organic material decomposition agent in organic fertilizer fermentation, and belongs to the technical field of organic waste resource utilization. According to the decomposition agent, in-situ microorganisms of an organic fertilizer fermentation system serve as an adaptation basis, and the functional strains are composed of liquid lactobacillus, lactobacillus, acetobacter aceti and clostridium. The thallus concentration of the liquid lactobacillus is 5.0 * 10 < 8 >-1.0 * 10 < 9 > cfu / mL, the thallus concentration of the lactobacillus is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL, the thallus concentration of the acetobacter aceti is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL, and the thallus concentration of the clostridium is 2.5 * 10 < 8 >-5.0 * 10 < 8 > cfu / mL. The application process of the decomposition agent in organic fertilizer fermentation is optimized, rapid decomposition of organic materials is achieved through the synergistic metabolism effect of functional strains, and the fermentation period is remarkably shortened to 14 days. The application method is easy and convenient to operate, high in adaptability and suitable for organic fertilizer fermentation production of various organic materials such as livestock and poultry manure, straw and wood chips, and has extremely high industrial application value.

The invention discloses a feed additive for reducing the cholesterol content of pork as well as a preparation method and application thereof, and belongs to the technical field of feeds. The additive is prepared from the following components in percentage by mass: 10 to 20 percent of phytosterol, 8 to 15 percent of tea polyphenol, 5 to 10 percent of soybean isoflavone, 5 to 10 percent of bacillus, 5 to 8 percent of clostridium butyricum, 3 to 5 percent of lactobacillus fermentum, 2 to 5 percent of selenium yeast, 8 to 12 percent of xylooligosaccharide, 5 to 8 percent of chitosan oligosaccharide and the balance of corn starch. The preparation method comprises the steps of superfine grinding of the plant extract, microencapsulation activation of the probiotics, homogenization treatment of the functional oligosaccharide, low-temperature mixing and the like. When in use, the feed additive is added into basic ration according to the proportion of 0.5-1.5%. Through a ternary compound system of the plant extract, the probiotics and the functional oligosaccharide, endogenous synthesis is inhibited through multi-target synergism, catabolism is promoted, exogenous absorption is reduced, the cholesterol content of pork can be remarkably reduced by 23% or above, meanwhile, the growth performance and meat quality of pigs are improved, economic benefits are remarkable, and the feed additive is suitable for producing high-added-value healthy pork.

The present invention relates to the technical field of probiotic screening and application, and specifically to a strain of Lactobacillus rhamnosus and its application in preventing or treating dental caries and periodontal disease. The Lactobacillus rhamnosus has a deposit number of CCTCC NO: M2022171, and has a significant inhibitory effect on pathogenic bacteria such as Streptococcus mutans and Actinomyces viscosus that cause dental caries, and Aggregatibacterium semiactinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum that cause periodontal disease, and can be used as an oral probiotic to effectively prevent and improve oral diseases. The lysate of Lactobacillus rhamnosus can also effectively remove the biofilm formed by Streptococcus mutans and inhibit the growth and reproduction of Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Actinomyces viscosus, and Aggregatibacterium semiactinomycetemcomitans, and can be added to medicines and tooth care products for use.

It was found that bacteria belonging to the genus Clostridium induce accumulation of regulatory T cells (Treg cells) in the colon. Moreover, the present inventors found that regulatory T cells (Treg cells) induced by from these bacteria suppressed proliferation of effector T-cells. From these findings, the present inventors found that the use of bacteria belonging to the genus Clostridium or a physiologically active substance derived therefrom made it possible to induce proliferation or accumulation of regulatory T cells (Treg cells), and further to suppress immune functions.

The invention discloses a microbial flora for producing methane and organic acid by anaerobic degradation of biomass as well as a culture method and application of the microbial flora. The microbial flora comprises the following components in percentage by weight: 5%-30% of Clostridium, 2%-10% of Paludibacter, 1%-25% of Thermovirga, 2%-20% of DMER64, 2%-20% of Mesotoga, 1%-10% of Methanosaeta and 1%-10% of Methanobacterium. The culture method of the flora comprises the following steps: S1, taking pectin, straws and fallen leaves, drying, and grinding the straws into powder by using a mortar; s2, mixing pectin degrading bacteria and residual sludge, inoculating the mixture into a culture bottle, adding a culture medium, and adding the powder obtained in the step S1 into the culture medium; s3, mixed gas of nitrogen and carbon dioxide is introduced, the culture bottle is sealed, continuous culture is performed for 30-100 days at the temperature of 30-38 DEG C, and pectin and cellulose hydrolysis flora enrichment is performed; the microbial flora can quickly hydrolyze and convert biomass such as straws and fallen leaves into methane, acetic acid, propionic acid, butyric acid or hexanoic acid products; compared with conventional pretreatment modes such as alkali treatment and high-temperature treatment, the method has the advantages of high yield, mild operation conditions, low operation cost and the like.

The invention provides a combined biological agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The combined biological agent is prepared from a penicillium oxalicum fermentation solution, a chaetophyllum fermentum fermentation solution, a thiamine-solubilizing clostridium fermentum fermentation solution, a nitrogen-fixing functional bacterium fermentation solution, a phosphorus-solubilizing bacillus megaterium fermentation solution, a bacillus mucilaginosus fermentation solution and a pseudomonas aeruginosa fermentation solution. According to the combined biological agent, through the synergistic effect of multiple functional strains, the effective state concentration of heavy metal in soil is effectively reduced, meanwhile, the soil fertility is improved, the survival rate and colonization ability of the agent in an extreme environment are enhanced, the vegetation coverage rate is remarkably increased, and the soil quality is improved. And an efficient solution is provided for ecological restoration of the solid waste storage yard of the nonferrous metal mining area in the northwest arid region.

This invention provides Bacillus subtilis ( Bacillus subtle The application of strain A45 in the control of plant diseases, its application in the preparation of microbial agents for the control of plant diseases, and the prepared microbial agents. Bacillus subtilis A45 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33289. The liquid microbial agent containing Bacillus subtilis A45 provided by this invention effectively inhibits the cucumber target spot pathogen, *Clostridium multiflorum* (…). Corynespora cassicola The growth mechanism of this strain lies in its encoding a gene cluster for the synthesis of phytosalicylic acid, purchemin, subtilosin A, and surfactant. Among these, the surfactant synthesis gene cluster is unique to this strain, exhibiting a maximum sequence similarity of 82%. Experiments show that the liquid inoculant containing Bacillus subtilis A45 provided by this invention can effectively inhibit the growth of *Clostridium multiflorum*, the pathogen causing target spot disease in cucumbers. Corynespora cashew nut The growth of ) can be controlled by field application with a prevention and control effect of up to 80%.

The invention discloses a compound enzyme bacterium fermented agricultural and sideline product feed and a production process thereof, and relates to the technical field of biological fermentation, the compound enzyme bacterium fermented agricultural and sideline product feed is obtained by anaerobic fermentation of compound enzyme bacterium fermented agricultural and sideline products; the agricultural and sideline products are cotton straws, corn flour, cottonseed hulls, cottonseed meal and bran; the compound enzyme bacteria are obtained by mixing soft rot fungi, trichoderma, cellulase, clostridium, manganese peroxidase and lactic acid bacteria; according to the compound enzyme bacterium fermented agricultural and sideline product feed disclosed by the invention, the cotton byproducts are treated by adopting a microbial fermentation method, after microbial fermentation, straw lignin, hemicellulose, gossypol, crude fibers and other components, which are not suitable for being used as feed, in the cotton byproducts are degraded, and the anti-nutritional factor level in the feed is remarkably reduced; the nutritional value of the feed is greatly improved; the feed can be used as a total mixed ration, can be used for raising livestock such as cattle and sheep, greatly reduces the feeding cost, and solves the problem of low utilization rate of cotton by-products.

A chimeric endolysin ClyRD of the present disclosure has antibacterial activity against bacteria that may cause foodborne diseases. Therefore, the chimeric endolysin ClyRD may be used as a natural controlling agent, a therapeutic agent or a preventive agent in the food industry.