25 results about "Proteobacteria" patented technology

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

The invention discloses a planting method for improving the yield and quality of sweet potatoes, and relates to the technical field of crop planting and cultivation, the key points of the technical scheme are as follows: deep ploughing is performed on land, ridging planting is performed during planting, furrowing is performed in the middle of a ridge, organic fertilizer is applied, sweet potatoes are planted on two sides, and the organic fertilizer is prepared by anaerobic fermentation of organic household garbage; in the fruit expanding period of the sweet potatoes, selenium fertilizer is sprayed on leaf surfaces for four times. According to the method, the physicochemical properties of soil can be improved, the yield and quality of sweet potatoes can be improved, and the fertility of the soil can be improved by remarkably increasing the content of organic carbon, total nitrogen and total phosphorus in the soil, reducing the volume weight, adjusting the pH value to be alkalescent, enriching functional florae mainly including Proteobacteria and Bacteroida and reducing the content of heavy metals such as Cd, Cr and Pb, so that the yield and quality of the sweet potatoes can be improved, and the yield and quality of the sweet potatoes can be improved. Meanwhile, the selenium content and crude protein content of the sweet potatoes are improved under the condition of ensuring high yield.

Described herein are synthetic inducible promoters including 5′-(UP element)-(−35 element)-(spacer element)-(−10 element)-(discriminator element)-3′. Also included are vectors, α-Proteobacteria strains and γ-Proteobacteria strains including the synthetic inducible promoters. A Mobile-CRISPRi plasmid and methods of partially or fully knocking-down expression of a gene in α-Proteobacteria or γ-Proteobacteria are also described. Further included are methods of making an α-Proteobacteria or γ-Proteobacteria strain.

The invention relates to the technical field of nano materials, in particular to a multifunctional electrospun nanofiber with bacteriostatic and anti-tumor effects and a preparation method and application of the multifunctional electrospun nanofiber. The result of the specific embodiment of the invention proves that the polyethylene oxide / gellan gum-carvacrol electrospun nanofiber can prolong the shelf life of strawberries by 3-4 days, and effectively alleviates the weight loss ratio, hardness, acidity, color and other quality deterioration of strawberry fruits. Microbial colony structure analysis shows that PEO / GG-CAR treatment can effectively reduce the flora richness and species number of strawberries, inhibit related putrefying bacteria such as proteobacteria, and promote the relative abundance of anaerobic microorganisms such as firmicobacteria and the like to be improved at the same time. Principal component analysis proves that the electrospun nanofiber membrane added with the CAR can effectively regulate microbial communities of strawberries, so that the preservative and fresh-keeping effects are achieved. Meanwhile, the electrospun nanofiber membrane also has the effect of inhibiting cancer cells.

The application discloses acetic acid-producing proteobacteria and application thereof in preparation of feed additives. 35 strains with good fiber degradation function are obtained from Holstein cow rumen fluid through preliminary screening, and bran medium is used for screening the 35 strains, and it is found that 25 strains have the ability of converting complex carbon source into reducing sugar, and the most powerful strains of different genera are selected for in-vitro fermentation verification, and the fiber utilization efficiency of the strains on ruminant TMR is observed, and it is found that the acetic acid-producing proteobacteria (microbial preservation number is CGMCC No. 33516) has the most powerful function on feed dry matter degradation rate and fiber degradation rate, and can improve rumen fermentation liquid pH value, and significantly improve the content of acetic acid, isobutyric acid, butyric acid, valeric acid and total rumen volatile fatty acid. The acetic acid-producing proteobacteria provided by the application has application prospect in improving feed fiber utilization rate or improving animal digestion capacity.

The application discloses Slackia a new application of sp.D-G6, and particularly relates to Slackia application of sp.D-G6 bacterial powder in pig breeding, and belongs to the field of agricultural animal breeding and microbial technology. The bacterial strain has been preserved in the Guangdong Microbial Culture Collection Center on May 8, 2019, and the preservation number is GDMCC NO: 60661. Research shows that the bacterial powder has no obvious adverse effect on pigs, has good feeding safety, can promote pig feeding and weight gain, improve average daily feed intake and average daily weight gain, and improve growth performance. Further, the bacterial powder can regulate the microbial composition of the ileum of growing pigs, increase the abundance of Firmicutes and Lactobacillus, Lactobacillus and reduce the abundance of Proteobacteria and / or Escherichia-Shigella, Escherichia-Shigella and has application prospect of being developed into a new green feed additive.

The invention relates to a composite crust material for desertification control and a preparation method and application thereof. A composite crust material for desertification control comprises a kaolin substrate layer and a polymer covering layer, the two layers are combined through hypha cross-linking, and the kaolin substrate comprises kaolin, nano titanium dioxide and natural plant fibers. The thickness of the kaolin substrate layer is 5-15 mm; and the thickness of the polymer covering layer is 2-5mm. The hyphae are selected from at least one of proteobacteria, the hyphae are sphingomonas and helicobacter, and the dry weight of the hyphae is 0.1-0.3 g / m. According to the invention, the kaolin substrate layer supplements clay particles, optimizes pores and improves the water-retaining property; a cross-linked network of the polymer covering layer enhances adhesion and improves corrosion resistance; hyphae penetrate through the two layers to form biological bridging, so that the permeation-water retention contradiction is solved.

The invention provides a composite complete set of health-care product composition based on double extraction of gold and radix rehmanniae as well as a preparation method and application of the composite complete set of health-care product composition. The complete set of health-care product composition is constructed based on yin deficiency and internal heat and circadian rhythm regulation and control concepts of traditional Chinese medicine. The beverage set contains three kinds of a honeysuckle probiotic composite beverage, a Jinzhi double-extraction fermentation beverage and spina date seed composite particles. Through systematic compatibility, a specific'golden-land symbiotic fermentation 'process and a systematic time-sharing intervention scheme based on circadian rhythm, cell and animal experiments prove that the product can significantly inhibit release of inflammatory factors, improve the activity of antioxidant enzymes, relieve liver cell lipid accumulation and endoplasmic reticulum stress, improve the immunity of liver cells and improve the immunity of the liver cells. The traditional Chinese medicine composition is capable of improving anxiety-like behaviors and sleep structures, regulating intestinal flora structures, increasing abundance of beneficial bacteria such as phylum firmicalis and reducing abundance of harmful bacteria such as proteobacteria, so that an overall conditioning effect is achieved at multiple targets, and a systematic intervention scheme based on circadian rhythm is provided for improving the sub-health status of modern pressure people.

The application discloses a fermentation feed feeding method for promoting growth of Ictalurus punctatus and improving intestinal flora. When cultivated, the Ictalurus punctatus is fed with puffed compound feed and fermentation feed; every other week, 20% of the puffed compound feed is replaced by the fermentation feed, and the mixed feed is fed for one week, and the feed is fed twice a day in the morning and in the evening. When cultivated by the method, the weight of the Ictalurus punctatus in the experimental group with the added fermentation feed is higher than that in the control group, and the weight of the Ictalurus punctatus in the interval feeding group is the highest and is significantly higher than that in the control group. Because the fermentation feed is continuously added, the diversity and richness of the intestinal flora of the Ictalurus punctatus decrease, especially the abundance of the actinobacteria and cyanobacteria, and the relative abundance of the opportunistic pathogen proteobacteria is increased, thereby affecting the growth of the Ictalurus punctatus.

The present invention discloses an application of tea lovage polysaccharide in the preparation of a drug for regulating intestinal flora in inflammatory bowel disease, and belongs to the technical field of polysaccharide extraction process optimization and intestinal flora regulation. Specifically, the present invention provides an economical and efficient extraction process and new use of tea lovage polysaccharide for the preparation of a preparation or composition. By increasing the relative abundance of probiotic flora, reducing the relative abundance of harmful bacteria, regulating the structure of intestinal flora, etc., the symptoms of intestinal flora disorder in inflammatory bowel disease are alleviated and treated. Tea lovage polysaccharide improves ulcerative colitis in mice by upregulating the relative abundance of probiotics such as Bacteroides and Faecalibacterium prausnitzii, and downregulating the relative abundance of pathogenic bacteria such as Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia, thereby reversing the abnormal proliferation of bacteria in mice with inflammatory bowel disease and improving the disordered intestinal flora in mice with inflammatory bowel disease.

This invention discloses a method for enhancing the degradation of petroleum hydrocarbons in soil by endogenous microorganisms using goethite. Goethite is used as a biostimulant to activate the activity of endogenous functional bacteria in the soil in degrading petroleum hydrocarbons. The dominant phyla of these endogenous functional bacteria are Proteobacteria, Actinobacteria, and Firmicutes, with a relative abundance of 50%–80%. Under conditions of soil pH 6.0–9.0 and soil moisture content 20%–50%, the degradation rate of total petroleum hydrocarbons in the soil is ≥ 60%, and the degradation rate of components such as colloids and asphaltene is ≥ 45%.

The present application relates to the technical field of plant fermentation, in particular to a fermented product of Desmodium adscendens, a preparation method thereof and a Desmodium adscendens tea. The preparation method of the fermented product of Desmodium adscendens comprises: fermenting Desmodium adscendens by using Aspergillus. The taste of the fermented product of Desmodium adscendens prepared by the preparation method is changed from 'bitter and astringent' to 'fresh, sweet and mellow', and the flavor is changed from 'green grass fragrance' to 'fruit sweet fragrance'; meanwhile, the bioavailability of active ingredients and in-vitro activities such as antioxidant activity, alcoholism relief and liver protection are significantly improved; in addition, the fermented Desmodium adscendens performs excellently in regulating intestinal microecology, and can promote the proliferation of beneficial bacteria and inhibit the excessive growth of harmful Proteobacteria.

The application discloses a Pseudomonas capable of degrading microcystins, an immobilized bacterial agent and application, and particularly relates to Pseudomonas chengduensis DMC-X1, which is isolated and screened from surface water samples of a cyanobacterial bloom outbreak in Nanyi Reservoir in Nanjing County, Zhangzhou City, Fujian Province. The strain belongs to gamma-proteobacteria, has high degradation capacity for microcystins (MCs), and has a degradation capacity superior to most Pseudomonas reported at present, and can be used for treating water polluted by microcystins. In the application, sodium alginate is used as a carrier, CaCl2 is used as a crosslinking agent, and the Pseudomonas DMC-X1 is embedded to obtain an immobilized bacterial agent, which can degrade microcystins in a short time and can be recycled for multiple times. The immobilized bacterial agent is convenient to store and recycle, and has important application value.

The invention discloses application of enzyme powder prepared from bamboo shoots to improvement of ulcerative colitis, which comprises the following steps: S1, pretreatment culture and grouping: 72 C57BL / 6J male mice are adaptively fed for one week and then are divided into 6 groups, each group comprises 12 male mice, and the number of the male mice is 12; the method comprises the following steps: sequentially naming a normal control group (NC), a model group (DSS), a sulfasalazine group (SASP, 300 mg / kg), a square bamboo shoot enzyme low-dose group (CUJ-L), a square bamboo shoot enzyme medium-dose group (CUJ-M) and a square bamboo shoot enzyme high-dose group (CUJ-H); experimental data of mice show that the square bamboo shoot enzyme treatment can significantly improve colon shortening and DAI, improve weight loss and reduce the colitis symptom factor level; besides, the chimonobambusa quadrangularis ferment intervention improves the diversity and the structure of intestinal bacteria, the composition of intestinal microbiota is remodeled, the abundance of Firmicite gate is improved, and the abundance of Proteobacteria and Verrucomicrobiota, including Escherichia-Shiga, Akkermansia and other species, is reduced, and the application of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment is promoted. The intestinal metabolite and the intestinal flora have a close relationship; according to the method, 32 key metabolites are screened out through cecum metabolite analysis, and the key metabolites mainly relate to a Propionate metabolite metabolic pathway; the result shows that the square bamboo shoot enzyme has the potential of treating colitis.

The invention provides application of plant source monoterpene in activating soil nitrogen cycle and promoting plant growth, and relates to the technical field of agricultural biology. Pot experiments prove that the monoterpene Trichodesmic acid can remodel a rhizosphere microbial community structure, significantly enrich proteobacteria and actinomycetes flora, up-regulate expression of nitrogen cycle key functional genes, and push a soil nitrogen metabolism pathway to be converted from a nitrogen fixation dominant mode to a nitrification-assimilation dominant mode, so that the content of total nitrogen and alkali-hydrolyzable nitrogen in soil is significantly increased. The synergistic effect of improving soil nutrients and promoting crop growth is achieved, and a novel plant source substance and an application scheme are provided for improving soil fertility and crop growth.

The invention discloses application of ligusticum chuanxiong hort polysaccharide in preparation of medicines for regulating intestinal flora of inflammatory bowel diseases, belongs to the technical field of polysaccharide extraction process optimization and intestinal flora regulation, and particularly provides an economical and efficient extraction process and new application of the ligusticum chuanxiong hort polysaccharide, and the ligusticum chuanxiong hort polysaccharide is used for preparing a preparation or a composition. The intestinal flora disorder symptom in the inflammatory bowel disease state is relieved and treated by improving the relative abundance of probiotic flora, reducing the relative abundance of harmful bacteria, adjusting the intestinal flora structure and the like. Ligusticum wallichii polysaccharide up-regulates the relative abundance of probiotics such as bacteroides, clostridium praeparatum and the like and down-regulates the relative abundance of pathogenic bacteria such as proteobacteria, gamma proteobacteria, enterobacteriaceae, escherichia and the like so as to improve ulcerative colitis of mice, reverse abnormally proliferating bacteria in mice with inflammatory bowel diseases and improve the intestinal flora disorder state of the mice with inflammatory bowel diseases.

Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase / isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The application discloses application of Qijiao Shengbai capsules in preparation of medicines for regulating intestinal microorganism flora disorder. The Qijiao Shengbai capsule is composed of Xue Renshen, Huangqi, Ajiao, Danggui, Da Zao, Ku Shen and Yinyanghe. By using the Qijiao Shengbai capsule, the microorganism flora is increased in the level of Firmicutes, increased in the level of Proteobacteria, reduced in the level of Bacteroidetes, reduced in the level of Akkermansia muciphila, and reduced in the level of Actinobacteria; in the level of genus, the microorganism flora is increased in the level of Prevotella, increased in the level of Lactobacillus, increased in the level of Parabacteroides, reduced in the level of Bacteroides, reduced in the level of Enterobacter, and reduced in the level of Staphylococcus, so as to regulate the intestinal microorganism flora. The application provides a new idea for research and development of medicines for preventing and treating intestinal flora disorder.

The invention belongs to the technical field of monopterus albus breeding, and particularly relates to application of beta-glucan in promoting monopterus albus growth and / or enhancing disease resistance of monopterus albus. It is found and verified that beta-glucan can significantly improve the growth of ricefield eel and the activity of digestive enzyme in the intestinal tract and improve the tissue structure of the intestinal tract; besides, the intestinal metagenome shows that by adding the beta-glucan, the proportion of beneficial bacteria (such as proteobacteria flora and / or spirillum flora) in the intestinal tracts of the ricefield eels can be increased, the proportion of harmful bacteria (such as desulfurization vibrio) can be reduced, then the intestinal environment is optimized, the growth of the ricefield eels is promoted, and the disease resistance of the ricefield eels is enhanced.

Methods, tools and kits allowing genetic modification, involving in particular a nucleic acid sequence used to facilitate the transformation of bacteria of the phylum Firmicutes, the sequence comprising i) all or part of the sequence SEQ ID NO: 126 and ii) a sequence allowing the modification of the genetic material of a bacterium and / or expression, within a bacterium, of a DNA sequence partially or totally absent from the genetic material present within the wild-type version of the bacterium are provided. Also provided are genetically modified bacteria and uses thereof, in particular for producing a solvent, preferably on an industrial scale.

The present invention provides a method for ecological restoration of mangroves in high-salinity high-tide beaches, which includes the following steps: S1. Soil improvement, S2. Seedling cultivation, S3. Seedling planting, S4. Seedling replanting, S5. Setting up a bank protection device, S6. Pest and disease control. The soil structure is improved and the soil salinity is reduced by using a soil conditioner. The seedlings are salt-resistant acclimated by gradually increasing the salinity of the irrigation water to improve the salt resistance of the seedlings. The use of organic fertilizers, iron porphyrin, abscisic acid, and brassinolide provides nutrients for the seedlings, regulates the osmotic balance in the plants, regulates the opening and closing of stomata, and regulates the plasticity of the cell wall, thereby enhancing the salt resistance of the plants. By inoculating the roots of the seedlings with Thalassohalophilus sp. and Proteobacteria, a symbiotic relationship is formed with the roots of the plants to form a microbial community, improving the absorption efficiency of nutrients in the soil by the plants. The present invention improves the planting survival rate and the plant growth rate through the overall synergistic effect of multiple pathways, thereby enhancing the biodiversity.

This invention discloses a metabolomics-gut microbiome biomarker combination for the diagnosis of HIV-related asymptomatic neurocognitive impairment (ANI), comprising metabolomics biomarkers and gut microbiome biomarkers. The metabolomics biomarkers include plasma and fecal biomarkers. Plasma biomarkers include Asp-Asn and arachidonic acid, while fecal biomarkers include histidine, lysine, ornithine, and indolelactone. The gut microbiome biomarkers include phylum-level and genus-level biomarkers. Phylum-level biomarkers include Firmicutes, Actinobacteria, and Proteobacteria, while genus-level biomarkers include *Clostridium*, *Microbacterium*, *Klebsiella*, and *Prevotella*. This biomarker combination integrates the core differential features of metabolomics and gut microbiota, exhibiting high diagnostic specificity and sensitivity. It can effectively distinguish ANI patients from CI patients and healthy individuals, providing a clear target for HAND targeted intervention and possessing significant clinical application value.

The invention provides a compound microbial agent for repairing uranium-contaminated soil and application of the compound microbial agent for repairing the uranium-contaminated soil, and the compound microbial agent for repairing the uranium-contaminated soil is prepared by mixing micromycobacterium and bacillus cereus. Through microbial agent treatment, the inhibition effect on seed germination of the sudangrass is remarkably relieved, and the plant biomass, the stress-resistant enzyme activity, the uranium enrichment coefficient, the total biomass of the sudangrass, the total nitrogen content of soil and the rapidly available phosphorus content are also remarkably improved; in the aspect of uranium pollution remediation efficiency, the complex microbial inoculants can reach 20%; the activity of beta-glucosidase is respectively improved by 10.57%-42.47%, and the activity of urease and the activity of catalase are also synchronously improved; the abundance of proteobacteria and cyanobacteria in a microbial community structure is obviously increased. According to the technical scheme provided by the invention, a uranium pollution treatment mode of'functional fungicide-rhizosphere micro-ecological regulation-plant synergistic remediation 'is constructed. A new thought and method are provided for repairing the uranium-polluted soil, and a scientific basis and a feasible repairing scheme are provided for subsequent large-scale application.