15 results about "Proteobacteria" patented technology

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

The invention discloses a planting method for improving the yield and quality of sweet potatoes, and relates to the technical field of crop planting and cultivation, the key points of the technical scheme are as follows: deep ploughing is performed on land, ridging planting is performed during planting, furrowing is performed in the middle of a ridge, organic fertilizer is applied, sweet potatoes are planted on two sides, and the organic fertilizer is prepared by anaerobic fermentation of organic household garbage; in the fruit expanding period of the sweet potatoes, selenium fertilizer is sprayed on leaf surfaces for four times. According to the method, the physicochemical properties of soil can be improved, the yield and quality of sweet potatoes can be improved, and the fertility of the soil can be improved by remarkably increasing the content of organic carbon, total nitrogen and total phosphorus in the soil, reducing the volume weight, adjusting the pH value to be alkalescent, enriching functional florae mainly including Proteobacteria and Bacteroida and reducing the content of heavy metals such as Cd, Cr and Pb, so that the yield and quality of the sweet potatoes can be improved, and the yield and quality of the sweet potatoes can be improved. Meanwhile, the selenium content and crude protein content of the sweet potatoes are improved under the condition of ensuring high yield.

The application discloses acetic acid-producing proteobacteria and application thereof in preparation of feed additives. 35 strains with good fiber degradation function are obtained from Holstein cow rumen fluid through preliminary screening, and bran medium is used for screening the 35 strains, and it is found that 25 strains have the ability of converting complex carbon source into reducing sugar, and the most powerful strains of different genera are selected for in-vitro fermentation verification, and the fiber utilization efficiency of the strains on ruminant TMR is observed, and it is found that the acetic acid-producing proteobacteria (microbial preservation number is CGMCC No. 33516) has the most powerful function on feed dry matter degradation rate and fiber degradation rate, and can improve rumen fermentation liquid pH value, and significantly improve the content of acetic acid, isobutyric acid, butyric acid, valeric acid and total rumen volatile fatty acid. The acetic acid-producing proteobacteria provided by the application has application prospect in improving feed fiber utilization rate or improving animal digestion capacity.

The application discloses Slackia a new application of sp.D-G6, and particularly relates to Slackia application of sp.D-G6 bacterial powder in pig breeding, and belongs to the field of agricultural animal breeding and microbial technology. The bacterial strain has been preserved in the Guangdong Microbial Culture Collection Center on May 8, 2019, and the preservation number is GDMCC NO: 60661. Research shows that the bacterial powder has no obvious adverse effect on pigs, has good feeding safety, can promote pig feeding and weight gain, improve average daily feed intake and average daily weight gain, and improve growth performance. Further, the bacterial powder can regulate the microbial composition of the ileum of growing pigs, increase the abundance of Firmicutes and Lactobacillus, Lactobacillus and reduce the abundance of Proteobacteria and / or Escherichia-Shigella, Escherichia-Shigella and has application prospect of being developed into a new green feed additive.

The invention relates to a composite crust material for desertification control and a preparation method and application thereof. A composite crust material for desertification control comprises a kaolin substrate layer and a polymer covering layer, the two layers are combined through hypha cross-linking, and the kaolin substrate comprises kaolin, nano titanium dioxide and natural plant fibers. The thickness of the kaolin substrate layer is 5-15 mm; and the thickness of the polymer covering layer is 2-5mm. The hyphae are selected from at least one of proteobacteria, the hyphae are sphingomonas and helicobacter, and the dry weight of the hyphae is 0.1-0.3 g / m. According to the invention, the kaolin substrate layer supplements clay particles, optimizes pores and improves the water-retaining property; a cross-linked network of the polymer covering layer enhances adhesion and improves corrosion resistance; hyphae penetrate through the two layers to form biological bridging, so that the permeation-water retention contradiction is solved.

The invention provides a composite complete set of health-care product composition based on double extraction of gold and radix rehmanniae as well as a preparation method and application of the composite complete set of health-care product composition. The complete set of health-care product composition is constructed based on yin deficiency and internal heat and circadian rhythm regulation and control concepts of traditional Chinese medicine. The beverage set contains three kinds of a honeysuckle probiotic composite beverage, a Jinzhi double-extraction fermentation beverage and spina date seed composite particles. Through systematic compatibility, a specific'golden-land symbiotic fermentation 'process and a systematic time-sharing intervention scheme based on circadian rhythm, cell and animal experiments prove that the product can significantly inhibit release of inflammatory factors, improve the activity of antioxidant enzymes, relieve liver cell lipid accumulation and endoplasmic reticulum stress, improve the immunity of liver cells and improve the immunity of the liver cells. The traditional Chinese medicine composition is capable of improving anxiety-like behaviors and sleep structures, regulating intestinal flora structures, increasing abundance of beneficial bacteria such as phylum firmicalis and reducing abundance of harmful bacteria such as proteobacteria, so that an overall conditioning effect is achieved at multiple targets, and a systematic intervention scheme based on circadian rhythm is provided for improving the sub-health status of modern pressure people.

This invention discloses a method for enhancing the degradation of petroleum hydrocarbons in soil by endogenous microorganisms using goethite. Goethite is used as a biostimulant to activate the activity of endogenous functional bacteria in the soil in degrading petroleum hydrocarbons. The dominant phyla of these endogenous functional bacteria are Proteobacteria, Actinobacteria, and Firmicutes, with a relative abundance of 50%–80%. Under conditions of soil pH 6.0–9.0 and soil moisture content 20%–50%, the degradation rate of total petroleum hydrocarbons in the soil is ≥ 60%, and the degradation rate of components such as colloids and asphaltene is ≥ 45%.

The present application relates to the technical field of plant fermentation, in particular to a fermented product of Desmodium adscendens, a preparation method thereof and a Desmodium adscendens tea. The preparation method of the fermented product of Desmodium adscendens comprises: fermenting Desmodium adscendens by using Aspergillus. The taste of the fermented product of Desmodium adscendens prepared by the preparation method is changed from 'bitter and astringent' to 'fresh, sweet and mellow', and the flavor is changed from 'green grass fragrance' to 'fruit sweet fragrance'; meanwhile, the bioavailability of active ingredients and in-vitro activities such as antioxidant activity, alcoholism relief and liver protection are significantly improved; in addition, the fermented Desmodium adscendens performs excellently in regulating intestinal microecology, and can promote the proliferation of beneficial bacteria and inhibit the excessive growth of harmful Proteobacteria.

The application discloses a Pseudomonas capable of degrading microcystins, an immobilized bacterial agent and application, and particularly relates to Pseudomonas chengduensis DMC-X1, which is isolated and screened from surface water samples of a cyanobacterial bloom outbreak in Nanyi Reservoir in Nanjing County, Zhangzhou City, Fujian Province. The strain belongs to gamma-proteobacteria, has high degradation capacity for microcystins (MCs), and has a degradation capacity superior to most Pseudomonas reported at present, and can be used for treating water polluted by microcystins. In the application, sodium alginate is used as a carrier, CaCl2 is used as a crosslinking agent, and the Pseudomonas DMC-X1 is embedded to obtain an immobilized bacterial agent, which can degrade microcystins in a short time and can be recycled for multiple times. The immobilized bacterial agent is convenient to store and recycle, and has important application value.

The invention discloses application of enzyme powder prepared from bamboo shoots to improvement of ulcerative colitis, which comprises the following steps: S1, pretreatment culture and grouping: 72 C57BL / 6J male mice are adaptively fed for one week and then are divided into 6 groups, each group comprises 12 male mice, and the number of the male mice is 12; the method comprises the following steps: sequentially naming a normal control group (NC), a model group (DSS), a sulfasalazine group (SASP, 300 mg / kg), a square bamboo shoot enzyme low-dose group (CUJ-L), a square bamboo shoot enzyme medium-dose group (CUJ-M) and a square bamboo shoot enzyme high-dose group (CUJ-H); experimental data of mice show that the square bamboo shoot enzyme treatment can significantly improve colon shortening and DAI, improve weight loss and reduce the colitis symptom factor level; besides, the chimonobambusa quadrangularis ferment intervention improves the diversity and the structure of intestinal bacteria, the composition of intestinal microbiota is remodeled, the abundance of Firmicite gate is improved, and the abundance of Proteobacteria and Verrucomicrobiota, including Escherichia-Shiga, Akkermansia and other species, is reduced, and the application of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment is promoted. The intestinal metabolite and the intestinal flora have a close relationship; according to the method, 32 key metabolites are screened out through cecum metabolite analysis, and the key metabolites mainly relate to a Propionate metabolite metabolic pathway; the result shows that the square bamboo shoot enzyme has the potential of treating colitis.

The invention provides application of plant source monoterpene in activating soil nitrogen cycle and promoting plant growth, and relates to the technical field of agricultural biology. Pot experiments prove that the monoterpene Trichodesmic acid can remodel a rhizosphere microbial community structure, significantly enrich proteobacteria and actinomycetes flora, up-regulate expression of nitrogen cycle key functional genes, and push a soil nitrogen metabolism pathway to be converted from a nitrogen fixation dominant mode to a nitrification-assimilation dominant mode, so that the content of total nitrogen and alkali-hydrolyzable nitrogen in soil is significantly increased. The synergistic effect of improving soil nutrients and promoting crop growth is achieved, and a novel plant source substance and an application scheme are provided for improving soil fertility and crop growth.

Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase / isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The application discloses application of Qijiao Shengbai capsules in preparation of medicines for regulating intestinal microorganism flora disorder. The Qijiao Shengbai capsule is composed of Xue Renshen, Huangqi, Ajiao, Danggui, Da Zao, Ku Shen and Yinyanghe. By using the Qijiao Shengbai capsule, the microorganism flora is increased in the level of Firmicutes, increased in the level of Proteobacteria, reduced in the level of Bacteroidetes, reduced in the level of Akkermansia muciphila, and reduced in the level of Actinobacteria; in the level of genus, the microorganism flora is increased in the level of Prevotella, increased in the level of Lactobacillus, increased in the level of Parabacteroides, reduced in the level of Bacteroides, reduced in the level of Enterobacter, and reduced in the level of Staphylococcus, so as to regulate the intestinal microorganism flora. The application provides a new idea for research and development of medicines for preventing and treating intestinal flora disorder.

This invention discloses a metabolomics-gut microbiome biomarker combination for the diagnosis of HIV-related asymptomatic neurocognitive impairment (ANI), comprising metabolomics biomarkers and gut microbiome biomarkers. The metabolomics biomarkers include plasma and fecal biomarkers. Plasma biomarkers include Asp-Asn and arachidonic acid, while fecal biomarkers include histidine, lysine, ornithine, and indolelactone. The gut microbiome biomarkers include phylum-level and genus-level biomarkers. Phylum-level biomarkers include Firmicutes, Actinobacteria, and Proteobacteria, while genus-level biomarkers include *Clostridium*, *Microbacterium*, *Klebsiella*, and *Prevotella*. This biomarker combination integrates the core differential features of metabolomics and gut microbiota, exhibiting high diagnostic specificity and sensitivity. It can effectively distinguish ANI patients from CI patients and healthy individuals, providing a clear target for HAND targeted intervention and possessing significant clinical application value.