36 results about "Proteobacteria" patented technology

This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.

The invention discloses 3-substituted-1-methyl-quinazoline-2,4-dione compounds shown in formula 1, a preparation method and application of the 3-substituted-1-methyl-quinazoline-2,4-dione compounds. Through in-vitro anti-microbial activity test, it is found that these compounds have certain inhibitory activity on gram-positive bacteria (Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Bacillus subtilis), gram-negative bacteria (Escherichia coli, proteusbacillus vulgaris, Pseudomonas aeruginosa) and fungus (Nostoc, Candida Albicans). Inhibitory activities of partial compounds on partial tested strains are approximate to or even superior to those of existing drugs such as streptomycin sulfate and polyoxin D. The compounds are especially sensitive to methicillin-resistant Staphylococcus aureus, can be prepared into antibacterial and / or anti-fungal drugs, and have the advantages of simple preparation method, easily-accessible materials and low cost.

The invention discloses the application of flavonoid glycosides of varicoseline in improving intestinal barrier function, and belongs to the technical field of drug development. In the present invention, the main active ingredient of blalicius seeds - the flavonoid glycosides of blalicius, is applied to improve intestinal barrier function. This natural substance can significantly improve the intestinal barrier function of animals, reduce the serum endotoxin content of mice with impaired barrier function, increase the intestinal alkaline phosphatase level and tight junction protein expression, and short-chain fatty acids in feces Propionic acid and butyric acid content, and can adjust the balance of intestinal flora, reduce the relative abundance of Firmicutes, and increase the relative abundance of Bacteroidetes and Proteobacteria.

The invention discloses a method for improving Fenton's oxidation combined with solid microbial agents to restore organic-contaminated soil, adding sodium pyrophosphate or malic acid to the sieved soil, adding ferric sulfate solution, and then adding hydrogen peroxide; Proteobacteria, β‑proteobacteria, yeasts, Pseudopora cyanicola, Pleurotus pellagra, Trametesmetamorphus, Bacillus subtilis, photosynthetic bacteria and lactic acid bacteria are cultured in liquid medium; then inoculated with straw, peanuts The microbial strain carrier composed of shell, bran and sawdust is cultivated and dried to obtain a solid bacterial agent; the solid bacterial agent is added to the soil to maintain soil moisture for restoration. The method of the present invention adopts the method of improving the Fenton reaction combined with microorganisms, the degradation rate is very high, up to 85-98.5%, and the repair time is short; the reaction can be carried out under the neutral pH condition of the soil, without additional adjustment of the soil pH; Use Fe3+ instead of Fe2+ to avoid the instability of hydrogen peroxide during Fe2+ reaction.

The invention relates to a biomarker for detecting or assisting in detecting proton ray radiation, a kit, a detection method and application thereof, and belongs to the field of biotechnology. The present invention provides a microbial flora biomarker for detecting or assisting detection of proton ray radiation, said microbial flora including Cyanophyta Cyanobacteria , ε Proteus Epsilonbacteraeota , Anaerovorax , Helicobacter Helicobacter at least one of the In the present invention, by detecting the expression amount or relative expression amount of the microbial flora DNA, it is determined whether the object to be tested has been exposed to proton ray radiation, and timely accident rescue, wounded treatment and clinical application are of great significance to radiation treatment.

The invention discloses a fluorescent quantitative PCR detection kit and a method thereof for predicting sepsis infection. The kit includes: Bacteroidetes forward primer and reverse primer, Firmicutes forward primer and reverse primer, Proteobacteria forward primer and reverse primer, Actinobacteria forward primer and reverse primer Primers, PCR master mixes and reagents for fluorescent quantitative PCR detection methods. The invention provides reliable evidence of infection and the etiological type of infection for the diagnosis of sepsis by detecting the classification level structure of human intestinal flora, can be used for auxiliary diagnosis of clinical sepsis, and provides relevant antibiotic treatment for patients. Effective basis, good prospects for clinical application.

The present invention amplifies the xylose-inducible promoter in Sinorhizobium meliloti, conducts bioinformatics analysis and functional verification, and obtains a gene that can be widely used in Sinorhizobium meliloti, Zymomonas mobilis, Caulobacter crescentus, and denitrification pseudomonas. Xylose for Gene Expression, Genetic Genetic Manipulation, and Strain Improvement in α-Proteobacteria, Agrobacterium tumefaciens, Brucella abortus, Pseudomonas fluorescens, Rhizobium leguminosae, and Sinorhizobium sativaceae Inducible promoter, its nucleotide sequence is SEQ ID NO:1 or SEQ ID NO:2. The invention also relates to a plasmid vector containing a xylose-inducible promoter, a method for constructing a genetically engineered bacterial strain by using the promoter, a corresponding bacterial strain, and an application of host cells to induce and start expression of a target gene under xylose-inducing conditions.

The present invention relates to blood-derived luterial and a method for isolating and culturing the same. The luterial according to the present invention is a cell or cell-like structure having the following characteristics: (1) it is present in body fluids, including blood, sperm, intestinal juices, saliva, and cellular fluids; (2) it shows a positive staining with Janus green B, Acridine Orange and Rhodamine 123 in an immunofluorescence test; (3) in an optimal environment (pH 7.2-7.4), it has the property of expressing the genes homologous to beta-proteobacteria and gamma-proteobacteria, and has a size of 30-800 nm; (4) in an acidic environment, it has the property of expressing not only the genes homologous to beta-proteobacteria and gamma-proteobacteria, but also eukaryote-derived genes (particularly Streptophyta gene), and grows to a size ranging from 400 nm or more to 2000 nm or more; (5) it is involved in ATP production under normal conditions; and (6) it differs from mitochondria, completely differs from exosomes, and has fusion characteristics corresponding to those of an intermediary between a prokaryote and an eukaryote.