Application of flavonoid glycosides of blancia in improving intestinal barrier function

A technology of blancillin glycosides and intestinal barrier function, which is applied in the field of drug development, can solve problems such as poor effect, lack of safety assessment, and low immune system, and achieve the effect of improving intestinal barrier dysfunction

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also deficiencies in probiotic therapy (gas production, abdominal distension, constipation, headache, etc.) and low immune system, patients with intravenous intubation or acute pancreatitis should not take probiotics, and the safety assessment is still lacking
At present, some natural medicine treatment methods are adopted, but many medicines are not effective, but natural medicine is receiving more and more attention because of its mildness and safety.

Method used

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  • Application of flavonoid glycosides of blancia in improving intestinal barrier function
  • Application of flavonoid glycosides of blancia in improving intestinal barrier function
  • Application of flavonoid glycosides of blancia in improving intestinal barrier function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Preparation and administration of mouse model

[0031] The mice were randomly divided into 3 groups (n=8), namely the control group, the model group, and the flavonoid glucoside group. The normal group was fed with common feed (14.7% J, 13% fat), and the rest of the groups were fed with HFD (21.8% J, 60% fat, D12492), free to eat. After 8 weeks of dietary intervention, except for the normal group, the other two groups were given 120 mg / kg STZ (dissolved in 10 mM citrate buffer, pH 4.0) after fasting for 12 hours. Mice in the normal group were given injection buffer at the same frequency. Each group continued to maintain the current diet, and after one week blood was collected from the tail vein to measure the blood glucose level. When the fasting blood glucose (FBG) level was higher than 11.1 mmol / L, mice were used as T2DM models.

[0032] Each group continued to maintain the current diet. After 8 weeks, the model group was injected intraperitoneally wi...

Embodiment 2

[0033] Example 2: Application of Buliuxing flavonoid glycosides in improving intestinal barrier function in mice

[0034] After 6 weeks, the mice were taken out, anesthetized by injection of 10% chloral hydrate, blood was collected from the eyeballs, and the ileum tissue and feces of the mice were taken from the mice.

[0035] (1) Changes of endotoxin in mouse serum

[0036] After centrifuging the whole mouse blood at low temperature at 12000rpm, take the supernatant, and use the endotoxin assay kit to quantify the expression of endotoxin in the serum of several groups of mice, such as figure 1 As shown in Table 1, the serum endotoxin content in the ileum of the mice in the model group was significantly increased, but after administration of vaccinia flavone glycosides, the serum endotoxin content could be reduced to a normal level.

[0037] (2) Changes of alkaline phosphatase (IAP) in mouse ileum tissue

[0038] The expression of alkaline phosphatase (IAP) in the ileum tiss...

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PUM

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Abstract

The invention discloses the application of flavonoid glycosides of varicoseline in improving intestinal barrier function, and belongs to the technical field of drug development. In the present invention, the main active ingredient of blalicius seeds - the flavonoid glycosides of blalicius, is applied to improve intestinal barrier function. This natural substance can significantly improve the intestinal barrier function of animals, reduce the serum endotoxin content of mice with impaired barrier function, increase the intestinal alkaline phosphatase level and tight junction protein expression, and short-chain fatty acids in feces Propionic acid and butyric acid content, and can adjust the balance of intestinal flora, reduce the relative abundance of Firmicutes, and increase the relative abundance of Bacteroidetes and Proteobacteria.

Description

technical field [0001] The invention relates to the application of varicose flavonoid glycosides in improving intestinal barrier function, and belongs to the technical field of drug development. Background technique [0002] The intestinal barrier function mainly includes mechanical barrier, ecological barrier and immune barrier, specifically including intestinal flora, mucus layer, intestinal epithelial cells and intercellular junctions, and intestinal immune system. When the intestinal barrier is damaged, the release of bacterial metabolites such as endotoxins entering the blood circulation increases, and intestinal permeability increases, leading to systemic inflammatory responses and further promoting the occurrence and development of chronic diseases. [0003] Currently, probiotic therapy and drug therapy are commonly used methods to improve the intestinal barrier function. Probiotic therapy includes transplanting fecal bacteria or microecological preparations, which ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K36/36A23L33/105A23L2/52A23K10/30A61P1/00A61P37/04A61K131/00
CPCA61K31/7048A61K36/36A61P1/00A61P37/04A23L33/105A23L2/52A23K10/30A23V2002/00A23V2250/2116A23V2200/32A23V2200/324A23V2200/30
Inventor 邱丽颖孙姜楠戚梦婷候豹蔡维维闫宏孙佳凝
Owner JIANGNAN UNIV
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