The present invention provides an 
alcohol-free 
virus nucleic acid extraction kit based on a 
magnetic bead method. The 
alcohol-free 
virus nucleic acid extraction kit comprises a lysate, a magnetic beadsolution, 
proteinase K, a polyA solution, a deproteinized solution, a washing solution and a 
buffer solution. The present invention also provides a method for extracting 
nucleic acid by using the above kit. Firstly, a sample, the lysate and magnetic beads are mixed evenly, then nucleic acid molecules are captured while 
pyrolysis is conducted, and finally the special 
alcohol-free deproteinized solution and the washing solution are respectively used to remove impurities of proteins, salt ions and other impurities on surfaces of the magnetic beads. Compared with a conventional 
virus nucleic acidextraction method, the method is simple in operation steps, does not need single addition of binding liquid, realizes the simultaneous 
pyrolysis and binding, and co-extracts 
DNA and 
RNA; the whole operation process is free of alcohol or 
isopropyl alcohol, inhibitory substance residues are less, the experimental procedure is simple, and the whole process only takes about 40 minutes; and the method is safe and 
pollution-free, and free of toxic reagents of 
chloroform, 
phenol, etc., and can be operated manually or on an 
automation platform.