The present invention provides an alcohol-free virus nucleic acid extraction kit based on a magnetic bead method. The alcohol-free virus nucleic acid extraction kit comprises a lysate, a magnetic beadsolution, proteinase K, a polyA solution, a deproteinized solution, a washing solution and a buffer solution. The present invention also provides a method for extracting nucleic acid by using the above kit. Firstly, a sample, the lysate and magnetic beads are mixed evenly, then nucleic acid molecules are captured while pyrolysis is conducted, and finally the special alcohol-free deproteinized solution and the washing solution are respectively used to remove impurities of proteins, salt ions and other impurities on surfaces of the magnetic beads. Compared with a conventional virus nucleic acidextraction method, the method is simple in operation steps, does not need single addition of binding liquid, realizes the simultaneous pyrolysis and binding, and co-extracts DNA and RNA; the whole operation process is free of alcohol or isopropyl alcohol, inhibitory substance residues are less, the experimental procedure is simple, and the whole process only takes about 40 minutes; and the method is safe and pollution-free, and free of toxic reagents of chloroform, phenol, etc., and can be operated manually or on an automation platform.