The present invention provides an
alcohol-free
virus nucleic acid extraction kit based on a
magnetic bead method. The
alcohol-free
virus nucleic acid extraction kit comprises a lysate, a magnetic beadsolution,
proteinase K, a polyA solution, a deproteinized solution, a washing solution and a
buffer solution. The present invention also provides a method for extracting
nucleic acid by using the above kit. Firstly, a sample, the lysate and magnetic beads are mixed evenly, then nucleic acid molecules are captured while
pyrolysis is conducted, and finally the special
alcohol-free deproteinized solution and the washing solution are respectively used to remove impurities of proteins, salt ions and other impurities on surfaces of the magnetic beads. Compared with a conventional
virus nucleic acidextraction method, the method is simple in operation steps, does not need single addition of binding liquid, realizes the simultaneous
pyrolysis and binding, and co-extracts
DNA and
RNA; the whole operation process is free of alcohol or
isopropyl alcohol, inhibitory substance residues are less, the experimental procedure is simple, and the whole process only takes about 40 minutes; and the method is safe and
pollution-free, and free of toxic reagents of
chloroform,
phenol, etc., and can be operated manually or on an
automation platform.