46results about How to "Increase abundance" patented technology

The invention relates to the technical field of intelligent medical treatment, and discloses a pancreatic cancer evolution simulation and dynamic intervention system based on multi-model driving, and the system comprises a multi-source data fusion module which is used for accessing and processing patient data, and forming a multi-dimensional feature set; the model fusion construction module is integrated with a model coupler and can construct a patient-specific integrated calculation model; the twinborn calibration generation module is used for generating pancreatic cancer digital twinborn matched with the initial state of the patient; and the evolution simulation and intervention module is used for running the pancreatic cancer digital twins, simulating the natural evolution trajectory of the tumor and carrying out quantitative simulation on the input virtual treatment scheme effect. According to the method, by constructing the patient-specific digital twins, normal form transformation from static diagnosis to dynamic prediction is achieved, and a quantitative evaluation basis can be provided for selection of clinical treatment schemes.

The application discloses Lactobacillus reuteri and application thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, and relates to the technical field of biological medicine. The application provides application of Lactobacillus reuteri and / or a metabolite thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, wherein the Lactobacillus reuteri is Lactobacillus reuteri (HG001). Limosilactobacillus reuteri The Lactobacillus reuteri is separated from feces of healthy people and used for preparation of the medicine for treating diffuse large B-cell lymphoma, and it is found that the Lactobacillus reuteri is confirmed as a probiotic strain for enhancing the chemotherapy effect of diffuse large B-cell lymphoma and can improve side effects caused by cyclophosphamide treatment of diffuse large B-cell lymphoma; and the Lactobacillus reuteri and the cyclophosphamide have a synergistic effect in treatment of diffuse large B-cell lymphoma.

The invention discloses a synthesis method of a high-abundance C-13 ethylene oxide compound, which comprises the following steps: (1) carrying out reaction on 13CH3I and diphenyl sulfide to obtain [13C] diphenylmethyl iodide; and (2) reacting the [13C] diphenylmethyl iodide with a carbonyl compound to obtain the high-abundance [13C] ethylene oxide compound. Diphenyl sulfide is adopted to replace dimethyl sulfide, the obtained [13C] methyl diphenyl sulfonium iodide avoids C-13 loss in the subsequent reaction process, and the abundance of C-13 is effectively improved.

The invention discloses brown cattle bone marrow collagen polypeptide with an immunoregulation effect as well as a preparation method and application of the brown cattle bone marrow collagen polypeptide, and belongs to the technical field of animal-derived food processing. The preparation method comprises the following steps: pretreating brown cattle bone marrow; the method comprises the following steps: dissolving pretreated brown cattle bone marrow in water, firstly adding alkaline protease to carry out enzymolysis, then simultaneously adding collagenase U and basophilic protease into a system, carrying out coupled enzymolysis under the protection of nitrogen, then cooling the system to 0-4 DEG C, regulating the pH value to 3-4, then carrying out autolysis for 10-30 minutes, and finally carrying out enzyme deactivation; centrifuging an enzyme deactivation product, taking supernatant, adjusting the pH value to be neutral, concentrating, dialyzing and freeze-drying to obtain crude collagen peptide; and carrying out primary purification and secondary refining on the crude collagen peptide to obtain the collagen peptide. The preparation process is simple, the molecular weight of the prepared polypeptide is 366-1514 Da, and the polypeptide has immunoregulation and antioxidant activity, can be used for preparing food, health care products or medicines for improving immunity and resisting oxidation, and realizes high-value utilization of livestock and poultry byproducts.

This invention belongs to the field of pharmaceutical technology, and relates to the use of a combination of sour cherry powder and celery seed powder, and complexes containing this composition. Specifically, the sour cherry powder and celery seed powder composition is used in the preparation of drugs for treating or regulating intestinal flora imbalance caused by hyperuricemia. The preparation method of this composition is as follows: pitting sour cherries, freezing them separately with liquid nitrogen, grinding them into powder using a pulverizer, and sieving them separately to obtain sour cherry powder and celery seed powder; mixing the obtained sour cherry powder and celery seed powder at a ratio of (0.5~3):1. This invention discusses the diversity, richness, and evenness of intestinal flora through diversity analysis, community bar chart analysis, and Venn diagram analysis. The results show that this composition can increase beneficial bacteria (Prevotella, Mulliez, Lactobacillus, etc.), reduce harmful bacteria (Alternaria), improve the structure of intestinal flora, and maintain the balance of intestinal flora.

The invention provides a zirconium-based solid electrolyte and a preparation method and application thereof, the chemical composition of the inorganic zirconium-based solid electrolyte material is Lix + yZrCl4 + x (OH) y, x is more than or equal to 0 and less than or equal to 2, and y is more than 0 and less than or equal to 2. The hydroxyl group is introduced to induce the material to generate lattice distortion and form a two-phase mixed region, and the rotation characteristic of the hydroxyl group is utilized to reduce the steric hindrance of lithium ion transmission, so that the ionic conductivity is remarkably improved. Besides, the introduction of the hydroxyl group induces in-situ generation of a self-limiting stable interface layer rich in Cl when the electrolyte is in contact with a lithium negative electrode, so that side reaction is effectively inhibited, and direct stable matching of the zirconium-based solid electrolyte and the lithium negative electrode is realized.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

This invention provides a method for rapidly maturing newly reclaimed severely saline-alkali land, comprising: sequentially performing the following steps in the newly reclaimed severely saline-alkali land: returning straw to the field, burying underground pipes, digging open ditches, flood irrigation to leach salt, applying base fertilizer, rotating leafy vegetables, and applying foliar fertilizer. The base fertilizer is a root-growth promoting fertilizer, and the leafy vegetables are selected from one or more of amaranth, Chinese cabbage, spinach, or bok choy. This method for rapidly maturing newly reclaimed severely saline-alkali land combines leafy vegetable rotation with root-growth promoting fertilizer to achieve ecological restoration and improvement of the land, thereby achieving the goal of controlling saline-alkali soil and increasing the output benefits of saline-alkali agriculture.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

The application relates to a long-life electrochromic device with double-ion synergistic working and a preparation method thereof. The long-life electrochromic device with double-ion synergistic working comprises a first transparent electrode layer, an inorganic electrochromic layer, an electrolyte layer, a gel-based ion storage layer and a second transparent electrode layer which are sequentially stacked; the material of the electrolyte layer is at least one of aluminum salt, preferably aluminum silicate, aluminum phosphate, aluminum borate, aluminum perchlorate and aluminum chloride; and the gel-based ion storage layer is a gel-based solid-state electrolyte containing lithium salt.

The invention belongs to the technical field of biological agriculture, and particularly discloses application of graphene oxide to improvement of tea tree quality. According to the application of the graphene oxide in improving the quality of the tea tree, the tea tree is treated through the graphene oxide with an effective dose, the rhizosphere and phyllosphere microflora structures of the tea tree are adjusted, and then the quality is improved. The invention discloses application of graphene oxide to improvement of tea tree quality. The diversity and structure of tea tree rhizosphere microorganisms can be remarkably changed, catechin substances and special flavor substances are improved, the health function value of tea leaves is enhanced, astringency, freshness, acid and bitter balance of the tea leaves is optimized, and a mellow and harmonious unique flavor is formed.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

The invention relates to the technical field of compositions, and particularly discloses a composition for improving EoE and application thereof. The composition for improving EoE comprises choline, taurine and a selenium source. In the composition for improving EoE provided by the invention, each raw material component is reasonably designed, and the effect of remarkably improving EoE is achieved by fully exerting a synergistic effect. Research shows that the composition can significantly improve EoE, which is mainly reflected in that the composition can reduce infiltration and activation of eosinophilic granulocytes in esophageal tissues, effectively reverse esophageal epithelial hyperplasia, reduce levels of cell factors such as IL-13 and the like, improve abundance of bifidobacteria in intestinal tracts and reduce abundance of enterobacter.

This invention provides a strain of *Pseudomonas aeruginosa* RL76, its bacterial suspension, its preparation method, and its applications, belonging to the field of agricultural microbiology technology. The *Pseudomonas aeruginosa* RL76 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 26, 2026, with accession number CGMCC No. 37531. This invention utilizes a mixture of filter mud and soil for sugar beet cultivation. It was found that, under the same pathogen inoculation conditions, the filter mud treatment significantly alleviated the occurrence of damping-off disease in sugar beets compared to the non-filter mud treatment, suggesting that the filter mud plays a role in increasing the relative abundance of beneficial microorganisms in the soil. Therefore, this invention isolated a strain of *Pseudomonas aeruginosa* RL76 with highly efficient antagonistic activity against *Rhizoctonia solani* from the rhizosphere soil of sugar beets cultivated using a mixture of filter mud and soil, and for the first time discovered its effective control of sugar beet damping-off disease while also promoting sugar beet growth, representing a groundbreaking achievement.

The invention discloses goose plasma protein source oligopeptide capable of improving liver lipid metabolism disorder and application of the goose plasma protein source oligopeptide, and relates to the technical field of biology. The goose plasma protein source oligopeptide capable of improving liver lipid metabolism disorder is obtained through screening, and the amino acid sequence is GDWIVK. Animal experiments prove that the goose plasma protein source oligopeptide can effectively reduce the levels of triglyceride, total cholesterol and low-density lipoprotein cholesterol in serum and liver affected by high fat diet, increase the content of high-density lipoprotein cholesterol, reduce liver lipid deposition and improve pathological injury of the liver. Meanwhile, the structure and diversity of intestinal flora can be adjusted, the abundance of beneficial bacteria and the content of short-chain fatty acid in excrement are increased, the reaction of colitis is relieved, and the synergistic adjustment effect is achieved through an intestinal tract-liver axis.

This invention discloses the application of Jerusalem artichoke polysaccharide in the preparation of drugs for regulating intestinal flora, belonging to the field of intestinal flora drugs. The drugs for regulating intestinal flora use Jerusalem artichoke polysaccharide as the active ingredient. These drugs improve intestinal disorders by increasing the proliferation of beneficial intestinal bacteria and the content of short-chain fatty acids, metabolites of intestinal flora. The Jerusalem artichoke polysaccharide prepared by this invention contains uronic acid and pyranose, and is mainly composed of fructose and glucose, with an average molecular weight of 2228 Da. The preparation method of Jerusalem artichoke polysaccharide in this invention has high yield, low cost, and is suitable for industrial production.

The invention is applicable to the technical field of crude oil treatment, and provides a method for separating and screening crude oil degrading functional bacteria. Comprising the following steps: S1, collecting an environmental sample polluted by crude oil for a long time; s2, inoculating the environmental sample into a gradient carbon source inorganic salt enrichment culture medium for enrichment culture to obtain an enrichment culture solution; s3, performing gradient dilution on the enrichment culture solution, coating the enrichment culture solution on a selective solid culture medium containing crude oil, and culturing to obtain a single colony; s4, picking single colonies, inoculating the single colonies into a liquid culture medium containing crude oil, and determining the crude oil degradation rate of the single colonies; s5, screening out strains with the crude oil degradation rate higher than a preset threshold value, namely the crude oil degradation functional bacteria. According to the method, the crude oil is used as a unique carbon source, infectious microbes which can only utilize an easily degradable carbon source are eliminated from the source, rare strains which really have a crude oil-enzyme system are enriched, the abundance of target bacteria can be improved by 2-3 orders of magnitude through first passage, and the subsequent purification period is shortened by more than 50%.

The present application discloses a polysaccharide for preventing, treating or improving chronic constipation, and a preparation method and application thereof. The polysaccharide can improve or restore intestinal barrier integrity, increase the expression amount of colon tight junction proteins, regulate the proportion of intestinal microbe beneficial flora, increase the content of short-chain fatty acids, improve gastrointestinal motility, regulate intestinal oxidative stress, increase the fecal water content and intestinal transport rate of constipation subjects, reduce intestinal tissue damage, and the like, thereby being capable of being used for preventing, treating or improving chronic constipation. In addition, the polysaccharide has the advantages of high safety and good curative effect.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for enriching functional microbial communities in fermentation pit mud. Given that most functional microbial communities in fermentation pit mud are anaerobic fermenting bacteria, their isolation and cultivation are difficult, limiting the improvement of pit mud. This invention provides a method for enriching functional microbial communities in fermentation pit mud, comprising the following steps: inoculating a pit mud suspension into a yellow water culture medium, then adding fortified Daqu (a type of starter culture), and culturing under anaerobic conditions to obtain a pit mud culture solution. Then, the pit mud culture solution is subjected to multiple rounds of inoculation and expansion. The pit mud culture solution obtained by this method shows a significant increase in the number of functional microbial communities *Methanococcus sanguinalis* and *Clostridium*, and an increased abundance of *Caproiciproducens*, which metabolizes characteristic products of baijiu (Chinese liquor), promoting the synthesis of butyric acid and caproic acid, improving the quality of the pit mud, and benefiting subsequent brewing production.

This invention discloses the use of phytic acid in the preparation of drugs or functional foods for regulating intestinal flora imbalance caused by Streptococcus mutans, belonging to the field of biomedicine and microecological regulation technology. This invention is the first to discover that oral phytic acid can effectively inhibit the abnormal colonization of Streptococcus mutans in the intestine, restore intestinal flora diversity, increase the abundance of beneficial bacteria (such as Bifidobacterium and Lactobacillus), reduce the proportion of pro-inflammatory bacteria, and improve intestinal barrier function and inflammatory status. This invention provides a new strategy for regulating intestinal flora imbalance caused by Streptococcus mutans.

The present application relates to a kind of modified biochar and its preparation method and application, first step, using bamboo to prepare biochar;Second step, preparation modified biochar;Third step, take anaerobic ammonia oxidation flocculation sludge in reactor, and add modified biochar in reactor.Using the method of the present application, the addition of modified biochar promotes the rapid granulation of anaerobic ammonia oxidation flocculation sludge can improve the denitrification activity and stability of anaerobic ammonia oxidation system at the same time, quickly realize the granulation of anaerobic ammonia oxidation flocculation sludge, and improve the relative abundance of anaerobic ammonia oxidation bacteria, has good theoretical guiding significance and practical application value for the cultivation of anaerobic ammonia oxidation granular sludge.

The application discloses a culture device for Mesocyclops leuckarti and application thereof, and belongs to the technical field of fish culture. The culture device for Mesocyclops leuckarti comprises a cylinder, a top cover and a bottom cover, the top cover is movably connected with the top of the cylinder, and the bottom cover is fixedly connected with the bottom of the cylinder; a collecting pipe is arranged in the cylinder, the upper portion of the collecting pipe is a silk screen, the lower portion of the collecting pipe is a closed pipe, and a first light source is arranged in the collecting pipe; a second light source and a third light source are arranged on the inner side of the cylinder, and a temperature regulator and an oxygenator are arranged on the lower portion of the inner side of the cylinder. The culture device can realize large-scale continuous high-density culture of Mesocyclops leuckarti, can be applied to preparation of a biological prevention and treatment device for fish white spot disease, can reduce the abundance of water body Paramoecium caudatum and thus reduce the probability of fish suffering from the white spot disease, can provide other food sources for the fish, is beneficial to growth and development of the fish, and enriches the aquatic food web.

The invention provides a composition for reducing the age of the intestinal tract, an oral composition formed by the composition and application, the composition for reducing the age of the intestinal tract comprises probiotics and prebiotics, and the probiotics are bifidobacteria; the prebiotics comprise two or more than two of polydextrose, isomaltooligosacharide and galactooligosaccharide. The composition can effectively influence and change the composition of intestinal bacteria of a body, promote the abundance of core characteristic bacteria representing young intestinal tracts to be increased and inhibit the growth of bacteria representing aging intestinal tracts, and people tests prove that the composition can effectively change the intestinal flora structure tending to be aged, reduce the age of the intestinal tracts and improve the immunity of the intestinal tracts. The intestinal tract becomes young.

The application discloses a DNA sulfur-modified bacillus subtilis and application thereof in alleviating alcoholic diseases, and relates to the field of biochemistry.The application provides a DNA sulfur-modified bacillus subtilis, which is named as Bacillus subtilis E10-1 and has a preservation number of CCTCC NO: M20253036.It is verified through animal experiments that the DNA sulfur-modified bacillus subtilis E10-1 is more resistant to alcohol than a strain in which sulfur modification is knocked out, can reduce the levels of indexes such as glutathione pyrotransaminase, glutathione transaminase and urea nitrogen in serum of a host after drinking alcohol, and the level of interferon gamma, can increase the levels of total protein, superoxide dismutase and glutathione peroxidase, can alleviate oxidative stress damage caused by alcohol, can reduce inflammatory reactions of various organ tissues, can maintain normal physiological functions of cells, can repair intestinal barriers, and can maintain intestinal flora homeostasis, so that alcoholic related diseases can be prevented and / or treated.

The application discloses a marker for screening aggressive social harmful behavior, a screening method and device and a storage medium, and relates to the technical field of aggressive social behavior screening. The reagent comprises a reagent for detecting relative abundance data of a microbial combination at the genus level. When the 13 kinds of bacteria are used for screening aggressive social harmful behavior, the screening accuracy is extremely high, and the screening method is non-invasive and objective. When the 13 kinds of bacteria are combined with social demographic information as screening factors of aggressive harmful social behavior, the screening accuracy is also extremely high. The application provides a new way for screening and intervention of aggressive social harmful behavior.

The utility model relates to the technical field of sampling detection, in particular to a portable microorganism enrichment device which comprises a box body, a sample filtering assembly, a liquid collecting tank, micro pump equipment and a drying bottle are integrated in an inner cavity of the box body, and the bottom end of the sample filtering assembly is communicated with the liquid collecting tank through a water pipe. The drying bottle is connected with the liquid collecting tank and the micro pump device through air pipes, and the surface of the liquid collecting tank is connected with a drainage pipe with a valve. When target microorganisms in a water body are enriched, sample liquid only needs to be injected from the liquid inlet pipe, and the equipment can perform two-stage filtration through the plankton net and the water system microfiltration membrane, so that the concentration of the enriched microorganisms is improved, and the accuracy of later characteristic identification is improved; a compact integrated design is adopted in the structure of the device, so that a worker can conveniently carry the device; meanwhile, the filtrate is regularly discharged through a drainage pipe with a valve below, so that the stable operation of the device is effectively guaranteed, and the operation convenience is improved.

The invention discloses an enzymolysis-fermentation synergistic biological peptide feed additive and a preparation method, and relates to the technical field of feed additives, the enzymolysis-fermentation synergistic biological peptide feed additive comprises a fermented wet material, and the fermented wet material comprises 100 parts of rapeseed meal, cellulase, pectinase, neutral protease, a complex microbial inoculant, astragalus polysaccharide, xylooligosaccharide, linoleic acid, a traditional Chinese medicine decoction and a proper amount of hydrochloric acid; and the complex microbial inoculant is compounded from lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis. The feed additive is prepared from rapeseed meal, cellulase, neutral protease, astragalus polysaccharide and other components, an enzymolysis-fermentation synergistic biotransformation process is realized, the problem that a single additive is difficult to meet multiple requirements of nutrition, immunity and the like at the same time is solved, the nutritional value and utilization efficiency of feed raw materials are improved, and the feed additive is suitable for industrial production. The immunity and disease resistance of animals are improved.

This invention belongs to the fields of bioengineering, environmental engineering, and clean energy technology, and discloses a method and its application for improving the enrichment rate of biofilm at the anode of a microbial electrolysis cell. The method includes the following steps: constructing a microbial electrolysis cell reactor; adding inoculum to the reactor; adding PBS buffer nutrient solution and acclimatization substrate to the reactor; purging the air from the reactor to induce an anaerobic state; applying a constant micro-voltage and connecting a resistor in series to the anode and cathode of the reactor; culturing microorganisms at a constant temperature of 25-35℃, monitoring the voltage across the series resistor in real time, and repeating steps S3-S7 when the output voltage drops below 20mV, with the proportion of acclimatization substrate gradually increasing in each cycle. This invention, through a gradient acclimatization scheme that gradually increases the proportion of acclimatization substrate, can enrich dominant bacterial communities, resulting in biofilms with complete bacterial structure and high abundance of functional bacteria.

This invention relates to the field of biotechnology, specifically to a method for constructing high-throughput sequencing libraries, a kit, and a method for detecting aminoacylated tsRNA. The method for constructing high-throughput sequencing libraries includes the following steps: (1) first-level enzymatic treatment; (2) second-level chemical treatment; (3) third-level enzymatic repair; (4) library construction. This invention constructs a triple cascade treatment system of "specific enzyme digestion labeling - denaturation deaminoacylation - enzymatic modification and repair", which overcomes the three major technical bottlenecks that have long existed in the sequencing of transport tRNA-derived fragments: "signal missed detection", "full-length truncation" and "confusion of active state".

The application belongs to the technical field of biology, and specifically discloses a method for relieving the adverse effects of glufosinate-ammonium on bumblebees by using astaxanthin. It is found that feeding astaxanthin to bumblebees can effectively relieve the adverse reactions caused by glufosinate-ammonium residues; in terms of the content of active oxygen in the intestinal tract, astaxanthin can significantly eliminate the active oxygen caused by glufosinate-ammonium; in terms of feeding behavior, astaxanthin can significantly restore the reduced feeding amount of bumblebees caused by glufosinate-ammonium exposure; in terms of the integrity of the midgut, astaxanthin can significantly relieve the dissolution of the peritrophic membrane of the midgut of bumblebees caused by glufosinate-ammonium exposure; and in terms of the intestinal microbial layer, astaxanthin can restore the level of core bacteria of bumblebees caused by glufosinate-ammonium exposure. The application also provides bumblebee feed for relieving the toxicity of glufosinate-ammonium and the application of astaxanthin in the preparation of a preparation for relieving the adverse effects of glufosinate-ammonium on bumblebees.