182results about How to "Increase abundance" patented technology

The invention discloses bifidobacterium animalis subsp. Lactis BLa79 as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium animalis subsp. Lactis BLa79 is CGMCC (China General Microbiological Culture Collection Center) NO. 32426, and the preservation number of the bifidobacterium animalis subsp. Lactis BLa79 is CGMCC NO. 32426. Based on the strain, the invention also provides application and related products of the strain. The BLa79 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora, can effectively relieve ulcerative colitis, and can relieve colitis by increasing body weight, reducing DAI score, reducing spleen index, inhibiting colonic atrophy, reducing proinflammatory factor level and promoting SIgA secretion.

The invention discloses a method for improving an acidic clayey soil plough layer, which comprises the following steps of: 1, after autumn rice is harvested, draining water in a rice field, and carrying out conventional tillage, soil turning and leveling on the rice field for later use; 2, green manure planting is conducted in the suitable green manure sowing period from October to November; 3, in the full-bloom stage, green manure is smashed, turned, pressed and returned to the field, lime and organic fertilizer are spread, the rice field is deeply ploughed at a time, and the green manure, the lime and the organic fertilizer are deeply buried in soil of the rice field; 4, the rice field is irrigated and soaked in water for a period of time, and then rice is planted. The method can inhibit and stabilize acid, improve the buffer performance of the soil, effectively expand a deep ploughing layer, improve the physical structure of the soil and improve the utilization rate of rainfall resources by the soil, so that the purposes of minimal ploughing, high yield and farmland protection are achieved.

The invention discloses lactobacillus reuteri CCFM1288 for relieving depressive mood and application of the lactobacillus reuteri CCFM1288, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1288 has the tryptophan metabolism capability, and can survive for 7 days in the intestinal tract; after the lactobacillus reuteri CCFM1288 intervenes a depressive mouse, anxiety-like and depression-like behaviors of the mouse can be obviously relieved, flora alpha-diversity is improved, intestinal symbiotic bacteria are promoted to secrete indole substances, intestinal homeostasis is maintained, hypothalamic-pituitary-adrenal axis hyperfunction is relieved, dopamine and serotonin neurotransmitter nervous system functions are improved, and the effect of preventing and treating depression is achieved. The neurotransmitter release is promoted, and the anti-depression effect is achieved. In clinical tests, intervention of Lactobacillus reuteri CCFM1288 can significantly improve the depressive symptom of MDD patients, and a new safe alleviation approach is provided for depression treatment.

The invention relates to ackermania muciniphila Akk21 with hypoglycemic capacity and application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides an ackermania muciniphila strain Akk21 with a blood sugar reducing capability. The preservation number of the ackermania muciniphila strain Akk21 is CCTCC (China Center for Type Culture Collection) NO: M 20242779. The invention also provides hypoglycemic drugs, functional food for promoting lead excretion, health care products and a method for preventing and treating lead / arsenic pollution based on the strain Akk21. The Ackermania muciniphila Akk21 has excellent lead and arsenic tolerance and hypoglycemic capacity, can effectively reduce the blood lipid level of mice with type II diabetes, has a remarkable reduction effect on inflammation of the whole body of the mice, and can remarkably reduce the number of harmful flora in the intestinal tract by increasing the abundance of effective flora in the intestinal tract, so that the content of the harmful flora in the intestinal tract is reduced, and the content of the harmful flora in the intestinal tract is reduced. The species diversity and abundance of intestinal flora are improved.

The present invention relates to a method of increasing nicotinamide mononucleotide (NMN) and / or nicotinamide adenine dinucleotide (NAD +) in the intestinal tract of a subject, the present invention relates to a method for treating a subject by administering to the subject a combination of at least one Bifidobacterium sp. Capable of growing on a sialylated HMO and having a functional de novo nicotinamide adenine dinucleotide (nad operon (nadC, nadD, and nadE) and at least one fucosylated oligosaccharide.

The invention belongs to the technical field of microorganisms, and particularly relates to clostridium butyricum and application thereof, and the preservation number of the clostridium butyricum is CGMCC (China General Microbiological Culture Collection Center) No.27853. According to the present invention, with the application of the bacterial strain, the species diversity of the host intestinal flora can be significantly reduced, the Firmicite / Bacteroidetes ratio can be significantly increased, the abundance of harmful bacteria such as Escherichia coli and Salmonella bacteria can be reduced, and the abundance of beneficial bacteria can be improved; in addition, the yield of butyric acid of the clostridium butyricum is larger than or equal to 1.5 g / L, and the activity of amylase in fermentation liquor of the clostridium butyricum is 16.04 + / -0.46 U / mL. When the feed additive is used in feed, intestinal functions of organisms can be effectively improved.

The invention discloses a preparation method and application of a composite nitrogen, phosphorus and sulfur removal autotrophic filter material, and relates to the technical field of sulfur autotrophic filter materials, and the preparation method comprises the following steps: stacking and combining a basic filter material, a catalyst loaded filter material, a strain-heterojunction loaded filter material and a magnesium oxide microsphere filter material from top to bottom, the composite nitrogen, phosphorus and sulfur removal autotrophic filter material is obtained; in the stacking combination process of the composite nitrogen, phosphorus and sulfur removal autotrophic filter material, the stacking thickness ratio of the basic filter material to the catalyst loaded filter material to the strain-heterojunction loaded filter material to the magnesium oxide microsphere filter material is 10: (3-5): (2-4): (4-5). The efficient and low-cost composite nitrogen, phosphorus and sulfur removal autotrophic filter material is developed according to the synergistic treatment requirements of nitrogen and phosphorus pollution and PNP pollution.

The invention discloses a psychrobacter haeli PN-Ta3 derived from turbot intestinal tracts and application of the psychrobacter haeli PN-Ta3, and belongs to the technical field of microorganisms. The psychrophilus haeli PN-Ta3 is separated from the intestinal tract of a healthy cultured turbot, and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, China, the preservation date is January 25, 2024, and the preservation number is CCTCC NO: M2024201. The psychrobacter nivalis PN-Ta3 disclosed by the invention has the beneficial effects that the psychrobacter nivalis PN-Ta3 has no pathogenicity to healthy turbots and has good colonization performance, and after the psychrobacter nivalis PN-Ta3 is prepared into freeze-dried bacterial powder and is mixed with and added to feed the turbots, not only can the growth and intestinal development of the cultured turbots be promoted, but also the intestinal microbial structure is optimized; the composition can also delay the morbidity and death of turbots caused by infection of aeromonas salmonicida BHAS-1.

The invention discloses an application of enzymatic casein in promotion of porcine endocrine cell proliferation, belongs to the technical field of animal nutrition and feed, and provides an application of enzymatic casein in promotion of porcine endocrine cell proliferation, which does not aim at diagnosis and treatment of diseases. The invention provides a method for promoting pig endocrine cell proliferation by using casein enzymolysis. The method comprises the following steps: adaptively feeding pigs with daily ration with the crude protein content of 13% for 7 days; mixing the daily ration with the crude protein content of 13% with the enzymolysis casein according to the mass ratio of 95.5: 4.5 to obtain the daily ration containing the enzymolysis casein, and feeding twice a day for 28 days. According to the invention, a certain proportion of casein hydrolysate is added into the feed to regulate and control microbe composition of small intestinal mucosa of growing pigs, so that proliferation of small intestinal endocrine cells is further regulated, the secretion level of gastrointestinal hormone is increased, the gastrointestinal transport function of animals is finally enhanced, the absorption of nutrient substances is accelerated, and the growth performance of the pigs is improved.

The invention discloses a detection method and application of whole genome 5-hydroxymethylcytosine, and relates to the technical field of biochemical detection. Comprising the following steps: 1, closing a 3 '-OH end of DNA to be detected; 2, preparation of glycosylated 5hmC DNA (Deoxyribose Nucleic Acid); 3, carrying out TdT mediated DNA (Deoxyribonucleic Acid) amplification; and 4, fluorescence measurement. According to the invention, a homogeneous and non-template dependent type signal amplification method for detecting the 5-hydroxymethylcytosine in the whole genome is constructed. The method is based on a specific enzyme catalytic reaction and a multiple signal amplification strategy, and has the characteristics of high sensitivity, good specificity, no need of a separation step, no sequence dependence in amplification and capability of detecting 5hmC at any position in a whole genome. In addition, accurate and sensitive detection of actual clinical samples can be realized.

The invention provides a multi-tower series connection method and device for boron isotope separation, and relates to the technical field of isotope separation. According to the multi-tower series connection method for boron isotope separation, four stages of low-temperature rectifying towers which are sequentially connected in series are adopted to achieve low-temperature rectifying separation of boron isotopes at the specific temperature and pressure, tower bottom materials of the previous stage of rectifying tower serve as feeding raw materials of the next stage of rectifying tower, and materials of all stages of rectifying towers are conveyed through pumps; after rectification of the four-stage rectifying tower, a 10BF3 product with abundance greater than 99% can be extracted from the tower bottom of the ultimate rectifying tower; and condensing the gas-phase material at the top of each stage of rectifying tower into liquid through a condenser, extracting the 11BF3 light component, and mixing and collecting the 11BF3 light component through a collecting tank. The method and the device are operated under the normal pressure condition, the boron isotope product can be continuously produced, the energy consumption is low, the production speed is high, the technological operation is simple, no side reaction exists, the product impurity is few, the purity of the boron isotope separated product can be ensured, the cost is low, and the method and the device have good industry prospects.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides bacillus subtilis and application thereof in prevention and control of corn diseases. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The invention also provides a microbial agent produced by using the SEB-34 strain, and the microbial agent can be used for preventing and treating plant diseases caused by fusarium graminearum or pelamys maydis. The bacillus subtilis provided by the invention is applied through seed treatment before sowing or root drip irrigation after seedling emergence, has the effects of preventing diseases, promoting growth and increasing yield, and can effectively reduce the occurrence of corn southern leaf blight and basal stem rot. The microbial agent provided by the invention is in a semi-dormant state, has relatively strong stress resistance, is more stable to store, ensures the effect, and effectively reduces the production and transportation cost at the same time. The invention also provides beneficial microbial resources for green prevention and control of corn diseases, and has good application prospect and high application value.

The invention relates to a method for composting by adopting hermetia illucens fecula. The method comprises the following steps: collecting hermetia illucens fecula and biological wastes; the method comprises the following steps of: mixing the raw materials, so that the carbon-nitrogen ratio in a mixture is (20-30): 1, spraying a microbial complex microbial inoculant, adding glucose, monopotassium phosphate, polysorbate, ferrous sulfate, manganese sulfate and copper sulfate, fully mixing, piling, covering with a heat-insulating film, and sealing to obtain a primary compost; collecting hollow bricks, laying a first hollow brick layer on the plane, enabling pores of the first hollow brick layer to be parallel to the horizontal plane, turning the primary compost to the first hollow bricks to form a first compost layer, flattening, laying a second hollow brick layer, sequentially carrying out the steps for a plurality of times until composting is completed, and covering the periphery of a compost body with a heat-insulating film to form the compost. And keeping for 10-30 days. The method can increase the abundance and strength of microbial metabolic pathways, increase the composting temperature, prolong the high-temperature time of the compost, accelerate the decomposition of the compost, and realize the harmlessness of the black soldier fly feces.

The invention provides a direct ammoxidation coupled autotrophic denitrification sewage treatment method and a bioreactor. The sewage treatment method by coupling direct ammonia oxidation with autotrophic denitrification comprises the following steps: sewage enters a continuous flow bioreactor, the bioreactor comprises a first-stage aerobic section, a second-stage anoxic section and a third-stage aerobic section which are arranged in sequence, a direct ammonia oxidation microbial agent is put into the first-stage aerobic section, and the third-stage aerobic section is put into the second-stage anoxic section; a ferrous sulfide autotrophic-heterotrophic denitrification filler is put into the second-stage anoxic section; the sewage comprises high-ammonia-nitrogen low-carbon-nitrogen-ratio wastewater; sewage staying in the third-stage aerobic section enters a secondary sedimentation tank and then is discharged. According to the method, no additional carbon source is needed, the sludge yield is low, and green, low-carbon and efficient treatment of the high-ammonia-nitrogen and low-carbon-nitrogen-ratio wastewater is realized.

The invention belongs to the technical field of drug delivery and food medicine, and particularly relates to resistant starch of intestinal targeted slow-release polyphenol and a preparation method and application of the resistant starch of the intestinal targeted slow-release polyphenol. Performing annealing recrystallization on the heat-dispersed high-amylose corn starch in an ethanol solution to obtain single-spiral structure resistant corn starch; and self-assembling the resistant corn starch with the single-spiral structure and the quinoa polyphenol extract in an alcohol-water system to obtain the intestinal targeted slow-release polyphenol resistant starch. According to the resistant starch of the intestinal targeted slow-release polyphenol prepared by the method disclosed by the invention, the specific starch spiral structure can realize the intestinal targeted controlled release of the polyphenol contained in the intestinal targeted slow-release polyphenol, so that the loss of phenolic compounds in the gastrointestinal digestion process is reduced, and the accurate and continuous release of the polyphenol compounds in the intestinal digestion stage is realized; the residence time of the polyphenol in the intestinal tract is prolonged, so that the bioavailability of the polyphenol is improved.

The invention provides a uric acid reducing composition containing fructus gardeniae and a preparation method of the uric acid reducing composition, and belongs to the technical field of medicines. The preparation method comprises the following steps: fermenting mulberry leaves, chicory, cape jasmine and kudzu vine roots through lactobacillus rhamnosus and bifidobacterium longum, separating probiotics from fermentation liquor, adding immobilized ionic liquid into the fermentation liquor, introducing carbon dioxide for adsorption, filtering, adding solids into ethanol, heating for desorption, concentrating to obtain an extract 1, precipitating filtrate with ethanol to obtain an extract 2, and carrying out freeze-drying to obtain the folium mori-chicory-cape jasmine extract. The preparation method comprises the following steps: embedding probiotics together with sodium alginate to prepare a uric acid-reducing embedding material, and uniformly mixing the uric acid-reducing embedding material with the extract 1, vitamin C and tea polyphenol to prepare the uric acid-reducing composition containing fructus gardeniae. The prepared uric acid reducing composition containing fructus gardeniae can promote uric acid excretion, inhibit sodium urate crystallization, improve the antioxidant effect, repair intestinal barriers, relieve inflammation, inhibit xanthine oxidase (XOD) activity and expression and reduce the blood uric acid level, has a good uric acid reducing effect and has a wide application prospect.

The invention provides a carbon-manganese-based composite material as well as a preparation method and application thereof. The carbon-manganese-based composite material is a MnO2-BC composite material formed by MnO2 and biochar, and MnO2 nanoparticles are loaded on the surface of the biochar; by adding 60mg of MnO2-BC / g.VS into a WAS anaerobic fermentation system, the anaerobic fermentation performance of the WAS can be greatly improved, and the degradation rates of polysaccharide, protein and SCOD (soluble chemical oxygen demand) are improved; the method can promote the directional enrichment of Desulfovibrio and Geobacter from the angles of energy metabolism regulation and electron directional transduction optimization, stably and efficiently solves the bottleneck problems of easy acid accumulation, poor flora synergistic effect, easy environmental stress in methane production and the like in the traditional WAS anaerobic fermentation, and has excellent application potential.

The invention belongs to the technical field of isotope separation, discloses a device and a method for preparing oxygen 18 and heavy water by using tap water, overcomes the defect of high energy consumption caused by a water rectification method, reduces the production cost, and is suitable for production of nuclear power and civil stable isotope oxygen 18 and heavy water. The device comprises a first large tower, a second large tower, a first tower group, a second tower group, a sixth small tower, a seventh small tower and an eighth small tower which are sequentially connected in series, the first large tower and the second large tower are used for pre-concentrating large-flow tap water entering the towers, and an intermediate material obtained at the bottoms of the towers is conveyed to the first tower group and the second tower group through a pump for moderate concentration; tower bottom concentrate is conveyed to the next-stage rectifying tower to be highly concentrated. According to the method, pre-concentration, primary concentration and concentration enrichment are carried out, intermediate products obtained at the tower bottom converge and enter a next-stage rectifying tower, then high-concentration enrichment is carried out, and finally oxy-18 water and heavy water are obtained.

The invention relates to the technical field of ecological restoration, in particular to a method for improving the transplanting survival rate of saplings in a hydro-fluctuation belt of a hydropower station reservoir area in a dry-hot valley area, which comprises the following steps: mixing a composite slow-release fungicide composition, saplings and seedling raising soil, putting the mixture into planting pits, and maintaining; the composition comprises the following raw materials: arbuscular mycorrhizal fungus powder, plant growth-promoting rhizobacteria, an alginate aqueous solution, a salicylate aqueous solution and a calcium chloride solution. The preparation method of the composition comprises the following steps: mixing the arbuscular mycorrhizal fungi, the plant growth-promoting rhizobacteria and the salicylate aqueous solution to obtain a mixture; dropwise adding the mixture into an alginate aqueous solution to form a mixed solution; and mixing the mixed solution with a calcium chloride aqueous solution, and curing to obtain the composite slow-release fungicide composition. The composite slow-release fungicide composition is mixed with saplings, so that the survival rate of the saplings is increased, and the ecological restoration rate of a hydropower station reservoir area in a dry and hot valley area is increased.

The invention discloses bifidobacterium breve with hypoglycemic capacity and application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides a bifidobacterium breve (Bifidobacterium breve) strain BBr37 with the blood sugar reducing capacity, and the preservation number of the bifidobacterium breve strain BBr37 is CGMCC (China General Microbiological Culture Collection Center) NO.32423. The invention also provides an application of the strain BBr37 in preparation of hypoglycemic drugs or health-care foods with an auxiliary hypoglycemic function or bacteriostatic agents or non-treatment-destination bacteriostasis, and a product and a method based on the strain BBr37. The strain BBr37 disclosed by the invention has a relatively strong inhibition effect on escherichia coli, staphylococcus aureus, salmonella, haemophilus parasuis, haemophilus parainfluenza, campylobacter jejuni and enterocolitis yarrowia, and can effectively achieve the effects of reducing blood sugar and regulating intestinal flora.

The invention discloses a copper ion-luteolin nano-composite and application thereof in treatment of inflammatory bowel disease, the obtained nano-composite has very strong stability and active oxygen scavenging ability, and has excellent anti-oxidation and anti-inflammatory ability, specifically, the nano-composite is negatively charged, can effectively target intestinal inflammation lesion, and can be used for treating inflammatory bowel disease, so that the copper ion-luteolin nano-composite can be applied to treatment of inflammatory bowel disease. Mucosal barrier repair is promoted, intestinal flora is regulated, and the inflammation microenvironment is remarkably regulated; the antioxidant and anti-inflammatory capacities of cells can be enhanced by regulating and controlling an oxidative stress pathway of a nuclear factor erythroid 2 related factor 2 / heme oxygenase-1 (Nrf2 / HO-1) and a nuclear factor kappa B (NF-kappa B) signal pathway; the compound shows a good treatment effect in a typical inflammatory bowel disease mouse model, including ulcerative colitis and Crohn's disease. The invention provides a new way for constructing a multi-layer treatment platform for treating the inflammatory bowel disease by using natural products.

The invention discloses low-molecular-weight fucoidin as well as a preparation method and application thereof. The low-molecular-weight fucoidin comprises 52%-65% of polysaccharide, 32%-47% of sulfate radical, 0-2% of glucuronic acid and 0-1% of protein. And the average molecular weight is 2.0-4.5 kDa. The low-molecular-weight fucoidin prepared by a specific extraction, separation and degradation method can resist obesity induced by high fat, the content of fasting blood-glucose, total cholesterol, triglyceride and low-density lipoprotein cholesterol is reduced, meanwhile, the relative abundance of obesity negative correlation flora is promoted, and the relative abundance of obesity positive correlation flora is inhibited; therefore, obesity development is prevented, and the compound has a huge application prospect in candidate drugs for preventing and / or treating obesity.

The invention discloses an optimization method for microscopic counting of free viruses in farmland soil, and belongs to the technical field of biological tests. According to the method, the test is carried out from the aspects of instrument selection, parameter setting, soil sample usage amount and microscopic observation key influence factors in the soil virus extraction test; analyzing the proportion of the farmland soil sample to the virus leaching solution, different oscillation instruments and parameter setting, and the influence of an anti-fluorescence quenching agent and an anti-fading solution in microscopic observation on the soil virus extraction efficiency and microscopic counting, and optimizing the farmland soil free virus microscopic counting process according to the analysis result. The invention provides an operation process suitable for the farmland soil virus fluorescence staining-microscopic counting method from instrument selection and parameter setting in soil virus extraction, soil sample usage amount and key influence factor control in microscopic observation. And a feasible technical reference can be provided for subsequent quantitative analysis and infection mode research of soil viruses.

The invention relates to the technical field of intelligent medical treatment, and discloses a pancreatic cancer evolution simulation and dynamic intervention system based on multi-model driving, and the system comprises a multi-source data fusion module which is used for accessing and processing patient data, and forming a multi-dimensional feature set; the model fusion construction module is integrated with a model coupler and can construct a patient-specific integrated calculation model; the twinborn calibration generation module is used for generating pancreatic cancer digital twinborn matched with the initial state of the patient; and the evolution simulation and intervention module is used for running the pancreatic cancer digital twins, simulating the natural evolution trajectory of the tumor and carrying out quantitative simulation on the input virtual treatment scheme effect. According to the method, by constructing the patient-specific digital twins, normal form transformation from static diagnosis to dynamic prediction is achieved, and a quantitative evaluation basis can be provided for selection of clinical treatment schemes.

The application discloses a goose plasma protein-derived oligopeptide capable of improving liver lipid metabolism disorder and application thereof, and relates to the field of biotechnology.The goose plasma protein-derived oligopeptide capable of improving liver lipid metabolism disorder is screened by the application, and the amino acid sequence is GDWIVK.Animal experiment verification shows that the goose plasma protein-derived oligopeptide can effectively reduce the levels of triglyceride, total cholesterol and low-density lipoprotein cholesterol in serum and liver affected by high-fat diet, increase the content of high-density lipoprotein cholesterol, reduce liver lipid deposition and improve liver pathological damage.Meanwhile, the goose plasma protein-derived oligopeptide can regulate the structure and diversity of intestinal flora, increase the abundance of beneficial bacteria and the content of short-chain fatty acids in feces, reduce the inflammatory response of colon, and play a synergistic regulation role through the intestinal-liver axis.

The present invention discloses a device and method for lifting seabed nutrients in a shellfish raft culture area. The shellfish raft includes a raft and a limit cable connected between the seabed and the raft. The device for lifting seabed nutrients in a shellfish raft culture area includes a raft arranged on the seabed, a plurality of groups of jet pipes are fixedly arranged on the raft, and a plurality of jet nozzles are opened on each group of jet pipes. The jet pipes are connected with an air duct, and an upper pressurizing mechanism and a lower pressurizing mechanism are connected to the air duct. The present invention uses the upper pressurizing mechanism and the lower pressurizing mechanism to provide power for the air duct to introduce gas above the sea surface into the seabed, forming an air curtain, so that an upwelling is generated in the raft culture area, thereby lifting the bottom water with a high nutrient salt content to the surface layer, providing bait microalgae for growth, improving the abundance of bait microalgae in the culture area, improving the primary productivity of the raft area, and thus improving the survival rate and yield of raft cultured shellfish. At the same time, it can increase the dissolved oxygen in the surface water, thereby playing a role in optimizing the seawater environment in the culture area.

The invention discloses a morchella esculenta-ganoderma lucidum cultivation method for improving continuous cropping soil of morchella esculenta, and relates to the technical field of morchella esculenta cultivation, and the method comprises the following steps: in the same land parcel, planting a first round of medium-mushroom morchella esculenta No.1 in November of the first year, and planting ganoderma lucidum after planting a second round of medium-mushroom morchella esculenta No.1 in November of the second year; after harvesting of the medium mushroom morchella No.1 every time, the soil is turned over and aired. By means of the method, the morchella planting frequency can be increased, the morchella yield can be increased, fungus communities in soil can be adjusted by planting ganoderma lucidum twice, vacant land parcels are not needed, the land utilization rate is increased, flora distribution can be adjusted, and ganoderma lucidum harvest can be obtained.

The application discloses Lactobacillus reuteri and application thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, and relates to the technical field of biological medicine. The application provides application of Lactobacillus reuteri and / or a metabolite thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, wherein the Lactobacillus reuteri is Lactobacillus reuteri (HG001). Limosilactobacillus reuteri The Lactobacillus reuteri is separated from feces of healthy people and used for preparation of the medicine for treating diffuse large B-cell lymphoma, and it is found that the Lactobacillus reuteri is confirmed as a probiotic strain for enhancing the chemotherapy effect of diffuse large B-cell lymphoma and can improve side effects caused by cyclophosphamide treatment of diffuse large B-cell lymphoma; and the Lactobacillus reuteri and the cyclophosphamide have a synergistic effect in treatment of diffuse large B-cell lymphoma.

The invention discloses a synthesis method of a high-abundance C-13 ethylene oxide compound, which comprises the following steps: (1) carrying out reaction on 13CH3I and diphenyl sulfide to obtain [13C] diphenylmethyl iodide; and (2) reacting the [13C] diphenylmethyl iodide with a carbonyl compound to obtain the high-abundance [13C] ethylene oxide compound. Diphenyl sulfide is adopted to replace dimethyl sulfide, the obtained [13C] methyl diphenyl sulfonium iodide avoids C-13 loss in the subsequent reaction process, and the abundance of C-13 is effectively improved.

The invention belongs to the field of modern agricultural technology and relates to a method for soil improvement, and specifically relates to a method for improving saline-alkali land, wherein a composite bacterial agent of Huajinpu alkaline halobacter BH‑8 and coal gangue is applied to saline-alkali land soil, wherein the concentration of Huajinpu alkaline halobacter BH‑8 in the composite bacterial agent is 1×10 9 ~3×10 9 CFU / mL, the concentration of coal gangue particles is 5-10g / L; the present invention uses Huajinpu alkaline halobacter and coal gangue to prepare a composite bacterial agent for saline-alkali land improvement for the first time. By comparing with the blank control group, coal gangue alone, and microorganisms alone, the combination of Huajinpu alkaline halobacter and coal gangue improves the soil fertility and various enzyme activities of saline-alkali land, and optimizes the structure and function of soil microbial communities in saline-alkali land. The method of the present invention is scientific and reliable, has good repeatability, has important scientific value, provides an important reference for saline-alkali land improvement, helps to play an important role in saline-alkali land improvement, and improves the efficiency of saline-alkali land improvement.