93 results about "Sodium pyruvate" patented technology

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

The application provides a culture medium and a culture method of ovarian cancer primary cells. The culture medium comprises an MST1 / 2 kinase inhibitor, sodium pyruvate, forskolin, epidermal growth factor, gastrin, fibroblast growth factor 7, nicotinamide, SB431542, and fetal bovine serum. Compared with the existing culture method, the in-vitro culture using the culture medium of the application has higher amplification efficiency; the culture of the ovarian cancer primary cells using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the ovarian cancer primary cell culture.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

The invention relates to an establishment method and application of a micropterus salmoides muscle cell line, and belongs to the technical field of cell culture. The invention provides a culture solution which takes an M199 culture medium as a basic culture medium and further comprises sodium pyruvate, hydroxyethyl piperazine ethanesulfonic acid, beta-mercaptoethanol, penicillin, streptomycin and fetal calf serum. The culture solution provides optimal conditions for micropterus salmoides muscle cell culture, the culture cost is also reduced, the micropterus salmoides muscle cell line is established by adopting the culture solution, the method is simple and easy to operate and high in repeatability, the established cell line is good in stability, continuous passage and ultralow-temperature cryopreservation can be realized, the cell division speed is high, the passage time is short, the adherence rate is high, and the method is suitable for large-scale popularization and application. In the method, the micropterus salmoides muscle primary cell culture time is short, the number of obtained cells is large, the activity is good, and passage can be carried out in only 8 days.

The invention relates to the technical field of NK cell culture, and discloses an NK culture medium containing hypotaurine and application of the NK culture medium to improvement of NK cell proliferation and killing activity, and the NK culture medium comprises a basic NK cell culture medium and hypotaurine; the basic NK cell culture medium does not contain hypotaurine and comprises amino acid, vitamins, inorganic salt, trace elements, D-glucose and sodium pyruvate. When the basic NK cell culture medium is an RPMI1640 culture medium, the concentration of the hypotaurine is 0.1-1 mM; when the basic NK cell culture medium is an IMDM culture medium, the concentration of the hypotaurine is 0.4-4 mM. According to the scheme, the hypotaurine is added into the basic NK cell culture medium to obtain the NK culture medium, the NK culture medium can increase the number of NK cells when the NK cells are cultured in vitro, and the killing activity of the cultured NK cells on tumor cells is remarkably improved.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

This invention belongs to the fields of applied environmental microbiology and agricultural biotechnology, and discloses ImALS, an acetyllactone synthase resistant to high concentrations of methoxylamine, its encoding gene, and its applications. Its nucleotide sequence is SEQ ID NO.1, with a full length of 1785 bp, encoding 594 amino acids, and its amino acid sequence is SEQ ID NO.2. ImALS shares less than 58.3% homology with previously reported acetyllactone synthases, thus representing a novel ALS gene. The acetyllactone synthase ImALS provided by this invention exhibits a specific activity of 0.52 mM / mg against sodium pyruvate. ImALS demonstrates strong resistance to the ALS inhibitor methoxylamine herbicide, with a half-maximal inhibitory concentration (IC50) of 1500 μM, indicating that the ImALS gene has significant application potential in methoxylamine-resistant transgenic engineering.

A micronutrient composition comprises of several combinations of individual ingredients in forming Mix 11, G12, G13 and core mix formulation. Core mix contains magnesium citrate, alpha-Ketoglutaric acid, L-Citrulline and Taurine. The Mix 11, G12 and G13 contains Magnesium Citrate, Alpha-Ketoglutaric Acid, Niacinamide, L-Glutamine, L-Citrulline, Sodium Pyruvate, Succinic acid, Vitamin B complex, Taurine, L-Cysteine, L-Methionine, L-Ascorbic Acid, Coenzyme Q10 or combination thereof. A method of administering and treating a specific disease which is due to one of an oxidative stress, impaired mitobiogenesis and ATP production in a mammal is disclosed.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

The invention discloses an efficient mouse small intestine epithelial cell isolated culture method. The method comprises the steps of fetal mouse small intestine tissue obtaining, flushing digestion, sediment obtaining through centrifugal separation, density gradient centrifugal purification, differential adhesion method purification, inoculated culture and culture medium replacement for further culture. During culture, a first culture medium and a second culture medium based on William's E basic culture media are sequentially used, and the first culture medium comprises fetal calf serum, EGF, L-glutamine, mannitol, methylprednisone, bovine insulin and double antibodies; the second culture medium is prepared from fetal calf serum, vitamin C, L-glutamine, resveratrol, sodium pyruvate, bovine insulin and double antibodies. The rapid adherent proliferation and long-term stable culture of the mouse small intestine epithelial cells on the 2D surface are realized through mutual cooperation of the two culture media, so that the separation and in-vitro culture method of the mouse small intestine epithelial cells, which is simple, easy to operate, stable and efficient, is established, and convenience is provided for metabolic research of various in-vitro drugs and inflammatory factors.

The present application belongs to the technical field of sewage treatment, and particularly relates to a composite carbon source additive and a preparation method thereof. The composite carbon source additive comprises the following components in parts by weight: 30-40 parts of sugarcane residue, 8-12 parts of polyvinyl alcohol derivative, 2-4 parts of functional filler, 5-8 parts of potassium citrate and 5-8 parts of sodium pyruvate. The preparation process of the polyvinyl alcohol derivative is as follows: polyvinyl alcohol, 1,3,5-benzene trisulfonic acid, triethylamine and 4-dimethylaminopyridine are added into dichloromethane for reaction, and then filtration, washing and drying are performed to obtain the polyvinyl alcohol derivative. The composite carbon source additive can slowly release and continuously and stably provide carbon source in the denitrification process, promote the denitrification process and improve the denitrification efficiency.

The present invention provides the use of pyruvate in the treatment of lipodystrophy syndrome, belonging to the technical field of endocrine and metabolic disease treatment. This invention proposes for the first time the use of pyruvate, such as sodium pyruvate and potassium pyruvate, in the treatment of lipodystrophy syndrome. It has been found that pyruvate can restore atrophic adipose tissue and improve the progression of diabetic complications and acute pancreatitis associated with hypertriglyceridemia, thereby increasing patient survival and improving overall prognosis.

A culture medium and a culture method for constructing prostate cancer organoids are provided. The culture medium includes a basic culture medium, and the following components with final concentrations: R-spondin1 15-200 ng / mL, Noggin 20-150 ng / mL, FGF2 5-15 ng / mL, FGF10 1-10 ng / mL, CHIR99021 0.5-1 μM, ALK inhibitor 0.25-1 μM, ROCK inhibitor 5-30 μM, nicotinamide 5-10 mM and sodium pyruvate 0.5-3 mM. The present culture medium and culture method can effectively improve the survival rate and formation efficiency of organoids through the compounding of each component.

The invention belongs to the technical field of biology, and particularly relates to an additive composition for CHO cell culture and application of the additive composition. The composition is prepared from 0.1 g / L to 0.5 g / L of carboxymethyl-beta-cyclodextrin, 0.4 g / L to 1.2 g / L of asparagine and 0.02 g / L to 0.1 g / L of sodium pyruvate. By adding the composition, the antibody yield can be effectively increased, meanwhile, the glycosylation modification proportion of G0F is increased, and a simple, convenient and effective yield and quality control method is provided for research, development and production of antibody drugs.

The invention discloses a CHO cell culture medium additive formula and a culture method. The CHO cell culture medium additive formula can be used for an upstream stage of in-vitro CHO cell culture and can remarkably reduce the proportion of Man5 in antibody glycosylation. The additives comprise an additive G1, an additive G2 and an additive G3; the additive G1 is a mixed mother solution with the concentration of 5g / L prepared from uridine, cytidine and hypoxanthine; the additive G2 is a mixed mother solution with the concentration of 1g / L prepared from sodium selenite and cadmium sulfate; the additive G3 is a mixed mother solution with the concentration of 100g / L prepared from asparagine, glutathione and sodium pyruvate. Compared with the prior art, the serum-free culture medium for culturing CHO cells provided by the invention has the advantages of simple components, low cost, no protein and no serum. Under the condition that cell growth and expression are not affected, the Man5 proportion in antibody glycosylation can be remarkably reduced.

The present invention discloses a method for preparing a radioactive probe precursor, which belongs to a chemical synthesis method. This method uses 5-bromoisatin and sodium pyruvate, which are common and inexpensive products on the market, as raw materials. FAPI-46 can be successfully prepared through 20 reactions, including a Pfitzinger reaction, high-temperature decarboxylation, tert-butyl protection of carboxyl groups, a C-N coupling reaction, removal of tert-butyl protection, methyl protection of tert-butyl groups, removal of methyl protection, and a condensation reaction. The shortest route requires only 10 reaction steps. Compared with methods reported in the literature, this method has good reproducibility, mild reaction conditions, few by-products, and is easy to purify. It is more practical and economical, and has obvious advantages.

This invention discloses a method for the simultaneous detection of sodium pyruvate in assisted reproductive culture media. High-performance liquid chromatography (HPLC) is employed, where sodium pyruvate in the sample is separated on a liquid chromatography column. Different columns and mobile phases are used in HPLC to separate components in the sample. Sodium pyruvate is separated in the column based on its different chemical properties and interactions with the mobile phase. This establishes an external standard method for quantitative analysis. This method solves the problems of complex operation, poor specificity, and low efficiency in existing detection techniques, achieving efficient separation and accurate quantification of sodium pyruvate. The operation is simple, with a single analysis time of approximately 15 minutes; the recovery rate is consistently within the range of 92.4%–108.6%. This invention provides a reliable analytical method for the quality control of assisted reproductive culture media, and is particularly suitable for the quantitative determination of sodium pyruvate in assisted reproductive culture media.

The application provides a kind of gamma delta T cell preparation and its preparation method and application, it is related to the field of biotechnology.The composition provided by the application includes: coenzyme Q10, vitamin C, human blood albumin, trehalose, sodium pyruvate and amifostine.Using the composition and related preparation provided by the application can effectively improve the killing ability of gamma delta T cells and maintain the function of gamma delta T cells, and can effectively inhibit tumor growth.

The invention discloses a method for promoting in-vitro maturation of sheep oocytes. The composition comprises an induction culture solution A and an induction culture solution B. The induction culture solution A and the induction culture solution B comprise a basic culture solution, and the induction culture solution A further comprises AMH; the induction culture solution B also contains FSH; the basic culture solution comprises an M199 basic culture medium, oestrus sheep serum, sodium pyruvate, luteinizing hormone, estradiol and a penicillin and streptomycin mixed solution. According to the composition and the method, through the synergistic effect of FSH treatment and AMH treatment, the maturation rate, the activity and the development potential of sheep oocytes are remarkably improved, so that the success rate of in-vitro fertilization and embryo transplantation is improved, an important scientific basis is provided for sheep reproduction and assisted reproduction technologies, related technologies are further optimized, and the application prospect is wide. The reproduction success rate is improved.

The present invention provides a bovine sex-sorted sperm sorting solution mediated by a small molecule compound and its application. The sperm sorting solution comprises a sperm sorting protection solution and a sex control functional component. The sperm sorting protection solution contains sodium chloride, potassium chloride, calcium chloride dihydrate, sodium dihydrogen phosphate, glucose, sodium pyruvate, sodium bicarbonate, penicillin-streptomycin, and phenol red. The sex control functional component includes creatine and a 24e drug. The concentration of creatine is 450 μM-550 μM, and the concentration of the 24e drug is 0.27 μM-0.33 μM. The 24e drug is a pyrido [3,2-d]pyrimidine-based TLR7&8 dual agonist. The present invention can meet the large demand for sex-sorted semen in production. Moreover, the sorting process is carried out under the suitable conditions for sperm and does not require complex treatment of sperm. It takes less time for sorting and causes less damage to sperm.

Provided is a brightening use of a composition including sodium pyruvate and one or more selected from the group consisting of urea and hydroxyethyl urea. Since the composition exhibits a synergistic brightening effect, even when a low concentration of sodium pyruvate is included, a brightening effect at the same level as when a high concentration of sodium pyruvate is used can be obtained. Therefore, the composition can be effectively applied in cosmetics, medicines, external preparations, and the like.

The invention provides a method for efficiently separating and purifying SAR11 bacteria, belongs to the technical field of environmental microorganisms, and aims to solve the problems that the SAR11 bacteria are difficult to culture, are easily inhibited by infectious microbes and the like. The method comprises the following steps: pre-filtering a seawater sample by using a 0.45 m mixed cellulose ester film, and enriching SAR11; the method comprises the following steps: by taking artificial seawater as a matrix, adding a mixture of sodium pyruvate, methionine, glycine and vitamins with specific concentration to prepare a culture medium, inoculating a sample into a 24-pore plate, and culturing under the conditions of 17-26 DEG C and darkness / 3000 / 5000lx; the method comprises the following steps: dyeing a sample for 45 minutes by SYBR Green I, and monitoring according to SAR11 light scattering and nucleic acid characteristics by using a flow cytometer (488nm laser excitation); target cells are subjected to expanding culture to obtain single colonies, and 50% glycerol is used for low-temperature breed conservation. According to the method, the separation success rate is increased, the period is shortened, and a support is provided for researching the physiological and ecological functions and the carbon cycle effect of the SAR11.

The invention belongs to the technical field of biology, and particularly relates to a lymphoma organoid culture medium and a culture method. The culture medium comprises the following components: 0.5 to 2 X of a GlutaMAX additive, 0.2 to 2 X of B-27, 0.5 to 2 X of N-2, 0.8 to 1.5 X of ITS-A, 1 to 10 mmol / L of sodium pyruvate, 50 to 300 ng / ml of WNT-3A, 2.5 to 10 mmol / L of nicotinamide, and the like which are dissolved in an RPMI 1640 culture medium. According to the culture method provided by the invention, in-vitro culture can be specifically carried out on the human lymphoma tissue, and in the culture process, an organoid which is similar to an in-vivo tumor from cell constitution and space structure is formed; the culture medium provided by the invention has clear components, is suitable for culturing different types of lymphoma organoid, and can provide a favorable culture method and research model for an in-vitro research platform in the disease field.

The invention relates to the technical field of aquaculture, in particular to a culture method and application of gill explants of micropterus salmoides. According to the technical scheme, the culture method of the gill explant of the largemouth bass comprises the following steps: culturing the gill explant of the largemouth bass by using an explant culture solution; the explant culture solution is formed by adding fetal calf serum, fish serum, three antibodies, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In cooperation with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be reserved, and the metabolic activity and functional integrity of the gill tissue can be maintained. According to the technical scheme, the technical problem that an ideal research model capable of maintaining the structural integrity of the gill tissue and accurately reflecting the functional characteristics of the gill tissue cannot be provided in the prior art can be solved. By means of the culture method, research models of ammonia nitrogen stress and the like can be efficiently constructed, and technical support is provided for micropterus salmoides gill tissue physiological mechanism research, disease prevention and control, environmental risk assessment and the like.

The invention discloses a method for amplifying and purifying CD8 < + > T cells, a culture medium and application of the culture medium, and relates to the technical field of cell purification, the culture medium for amplifying and purifying the CD8 < + > T cells comprises the following components: 5-25ng / mL of interleukin-2, 1mM of sodium pyruvate, 2mM of L-alanyl-L-glutamine dipeptide, 55mu M of 2-mercaptoethanol and the balance of water. The culture medium comprises 50-400ng / mL of anti-CD3 antibody, a basic culture medium, serum and antibiotics. The culture medium provided by the invention can effectively activate and proliferate CD8 < + > T cells, but cannot effectively promote proliferation and activation of CD4 < + > T cells and other cells, thereby playing a role in purifying the CD8 < + > T cells. The CD8 + T cells can be amplified and purified by incubating the mouse lymph node cells with the culture medium, the operation is simple and convenient, and the cost is low.