46 results about "Sodium pyruvate" patented technology

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention relates to the technical field of NK cell culture, and discloses an NK culture medium containing hypotaurine and application of the NK culture medium to improvement of NK cell proliferation and killing activity, and the NK culture medium comprises a basic NK cell culture medium and hypotaurine; the basic NK cell culture medium does not contain hypotaurine and comprises amino acid, vitamins, inorganic salt, trace elements, D-glucose and sodium pyruvate. When the basic NK cell culture medium is an RPMI1640 culture medium, the concentration of the hypotaurine is 0.1-1 mM; when the basic NK cell culture medium is an IMDM culture medium, the concentration of the hypotaurine is 0.4-4 mM. According to the scheme, the hypotaurine is added into the basic NK cell culture medium to obtain the NK culture medium, the NK culture medium can increase the number of NK cells when the NK cells are cultured in vitro, and the killing activity of the cultured NK cells on tumor cells is remarkably improved.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

This invention belongs to the fields of applied environmental microbiology and agricultural biotechnology, and discloses ImALS, an acetyllactone synthase resistant to high concentrations of methoxylamine, its encoding gene, and its applications. Its nucleotide sequence is SEQ ID NO.1, with a full length of 1785 bp, encoding 594 amino acids, and its amino acid sequence is SEQ ID NO.2. ImALS shares less than 58.3% homology with previously reported acetyllactone synthases, thus representing a novel ALS gene. The acetyllactone synthase ImALS provided by this invention exhibits a specific activity of 0.52 mM / mg against sodium pyruvate. ImALS demonstrates strong resistance to the ALS inhibitor methoxylamine herbicide, with a half-maximal inhibitory concentration (IC50) of 1500 μM, indicating that the ImALS gene has significant application potential in methoxylamine-resistant transgenic engineering.

A micronutrient composition comprises of several combinations of individual ingredients in forming Mix 11, G12, G13 and core mix formulation. Core mix contains magnesium citrate, alpha-Ketoglutaric acid, L-Citrulline and Taurine. The Mix 11, G12 and G13 contains Magnesium Citrate, Alpha-Ketoglutaric Acid, Niacinamide, L-Glutamine, L-Citrulline, Sodium Pyruvate, Succinic acid, Vitamin B complex, Taurine, L-Cysteine, L-Methionine, L-Ascorbic Acid, Coenzyme Q10 or combination thereof. A method of administering and treating a specific disease which is due to one of an oxidative stress, impaired mitobiogenesis and ATP production in a mammal is disclosed.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

The invention belongs to the technical field of biology, and particularly relates to an additive composition for CHO cell culture and application of the additive composition. The composition is prepared from 0.1 g / L to 0.5 g / L of carboxymethyl-beta-cyclodextrin, 0.4 g / L to 1.2 g / L of asparagine and 0.02 g / L to 0.1 g / L of sodium pyruvate. By adding the composition, the antibody yield can be effectively increased, meanwhile, the glycosylation modification proportion of G0F is increased, and a simple, convenient and effective yield and quality control method is provided for research, development and production of antibody drugs.

This invention discloses a method for the simultaneous detection of sodium pyruvate in assisted reproductive culture media. High-performance liquid chromatography (HPLC) is employed, where sodium pyruvate in the sample is separated on a liquid chromatography column. Different columns and mobile phases are used in HPLC to separate components in the sample. Sodium pyruvate is separated in the column based on its different chemical properties and interactions with the mobile phase. This establishes an external standard method for quantitative analysis. This method solves the problems of complex operation, poor specificity, and low efficiency in existing detection techniques, achieving efficient separation and accurate quantification of sodium pyruvate. The operation is simple, with a single analysis time of approximately 15 minutes; the recovery rate is consistently within the range of 92.4%–108.6%. This invention provides a reliable analytical method for the quality control of assisted reproductive culture media, and is particularly suitable for the quantitative determination of sodium pyruvate in assisted reproductive culture media.

The application provides a kind of gamma delta T cell preparation and its preparation method and application, it is related to the field of biotechnology.The composition provided by the application includes: coenzyme Q10, vitamin C, human blood albumin, trehalose, sodium pyruvate and amifostine.Using the composition and related preparation provided by the application can effectively improve the killing ability of gamma delta T cells and maintain the function of gamma delta T cells, and can effectively inhibit tumor growth.

The invention discloses a method for promoting in-vitro maturation of sheep oocytes. The composition comprises an induction culture solution A and an induction culture solution B. The induction culture solution A and the induction culture solution B comprise a basic culture solution, and the induction culture solution A further comprises AMH; the induction culture solution B also contains FSH; the basic culture solution comprises an M199 basic culture medium, oestrus sheep serum, sodium pyruvate, luteinizing hormone, estradiol and a penicillin and streptomycin mixed solution. According to the composition and the method, through the synergistic effect of FSH treatment and AMH treatment, the maturation rate, the activity and the development potential of sheep oocytes are remarkably improved, so that the success rate of in-vitro fertilization and embryo transplantation is improved, an important scientific basis is provided for sheep reproduction and assisted reproduction technologies, related technologies are further optimized, and the application prospect is wide. The reproduction success rate is improved.

The invention provides a method for efficiently separating and purifying SAR11 bacteria, belongs to the technical field of environmental microorganisms, and aims to solve the problems that the SAR11 bacteria are difficult to culture, are easily inhibited by infectious microbes and the like. The method comprises the following steps: pre-filtering a seawater sample by using a 0.45 m mixed cellulose ester film, and enriching SAR11; the method comprises the following steps: by taking artificial seawater as a matrix, adding a mixture of sodium pyruvate, methionine, glycine and vitamins with specific concentration to prepare a culture medium, inoculating a sample into a 24-pore plate, and culturing under the conditions of 17-26 DEG C and darkness / 3000 / 5000lx; the method comprises the following steps: dyeing a sample for 45 minutes by SYBR Green I, and monitoring according to SAR11 light scattering and nucleic acid characteristics by using a flow cytometer (488nm laser excitation); target cells are subjected to expanding culture to obtain single colonies, and 50% glycerol is used for low-temperature breed conservation. According to the method, the separation success rate is increased, the period is shortened, and a support is provided for researching the physiological and ecological functions and the carbon cycle effect of the SAR11.

The invention belongs to the technical field of biology, and particularly relates to a lymphoma organoid culture medium and a culture method. The culture medium comprises the following components: 0.5 to 2 X of a GlutaMAX additive, 0.2 to 2 X of B-27, 0.5 to 2 X of N-2, 0.8 to 1.5 X of ITS-A, 1 to 10 mmol / L of sodium pyruvate, 50 to 300 ng / ml of WNT-3A, 2.5 to 10 mmol / L of nicotinamide, and the like which are dissolved in an RPMI 1640 culture medium. According to the culture method provided by the invention, in-vitro culture can be specifically carried out on the human lymphoma tissue, and in the culture process, an organoid which is similar to an in-vivo tumor from cell constitution and space structure is formed; the culture medium provided by the invention has clear components, is suitable for culturing different types of lymphoma organoid, and can provide a favorable culture method and research model for an in-vitro research platform in the disease field.

The invention relates to the technical field of biological medicines, and provides a high VEGF protein / miR-210 extracellular vesicle induced by a serum-free combined culture medium and application of the high VEGF protein / miR-210 extracellular vesicle. According to the invention, high VEGF protein / miR-210 extracellular vesicles are obtained based on induction of a serum-free culture medium; the serum-free culture medium comprises a basic culture medium component combination: sugar-free RPMI1640 and MCDB131 in a volume ratio of 4: 1; the metabolic regulation additive is prepared by synergistically adding sodium pyruvate, cysteine and a proline hydroxylase inhibitor and taking alanine glutamine as an energy substitute; the growth factor is a combination of polyvinyl alcohol, 4-hydroxyethylpiperazine ethanesulfonic acid, ITS-X, epidermal growth factors and basic fibroblast growth factors, wherein the ITS-X, the epidermal growth factors and the basic fibroblast growth factors are added before use. CD63 and CD81 are specifically expressed on the surface of the prepared high VEGF protein / miR-210 extracellular vesicle, the high VEGF protein / miR-210 extracellular vesicle is rich in VEGF protein and miR-210, and the high VEGF protein / miR-210 extracellular vesicle is applied to targeted therapy of allergic rhinitis by inhibiting Th2 type immunoreaction.

This invention provides a method for preparing a thermosensitive gel for the prevention and treatment of nasal diseases and its application. By weight and volume percentage, it comprises recombinant human type III collagen, mussel adhesive protein, an anti-allergy agent, traditional Chinese medicine extracts, thermosensitive materials, and a sodium pyruvate-cocamidopropyl betaine ion-pair complex. This invention, by pre-forming the ion-pair complex, simultaneously solves the technical problems of existing nasal gels, such as poor anti-dilution ability, short adhesion time, and easy inactivation of active ingredients. The product is liquid below 30°C and can be sprayed for complete nasal coverage. At body temperature, it transforms into a gel within 2 minutes for long-lasting adhesion, exhibiting rapid anti-inflammatory, nasal patency-clearing, antipruritic, and mucosal repair effects. It is suitable for the prevention and treatment of allergic rhinitis and sinusitis, as well as nasal wound care, with significantly better efficacy than similar commercially available products.

The invention relates to the technical field of microorganisms. The invention particularly discloses a culture medium and a preparation method and application thereof. Each liter of the culture medium comprises 3-8 g of L-asparagine, 0.3-1 g of buffer salt, 8-15 g of a carbon source, 50-100 mL of glycerin, 0.01-0.1 g of ferric ammonium citrate, 0.1-0.5 g of magnesium sulfate, 1-5 g of citric acid and the balance of water. Wherein the carbon source is selected from one or more of sodium pyruvate, glucose and glucose monohydrate; the buffer salt is selected from monopotassium phosphate and / or sodium dihydrogen phosphate. The culture medium is simple in component, wide in source and low in cost, and low-cost culture of mycobacteria can be achieved.

The invention discloses a culture medium, a culture method and application of mouse hepatic sinus vascular endothelial cells. Belongs to the technical field of cell culture. Components of the culture medium are optimized, in the aspect of growth factors, conventional VEGF / bFGF combination is broken through, liver cell growth factors and platelet-derived growth factors are creatively compounded with the growth factors, cell proliferation is driven, and liver-derived specific phenotypes and functional integrity of LSEC are maintained. An anti-oxidation system composed of N-acetylcysteine, ascorbic acid, alpha-tocopherol and riboflavin is constructed, active oxygen is synergistically removed through multiple mechanisms, and the activity and stability of cells in long-term culture are improved. Sodium pyruvate is additionally supplemented as an energy substrate, and taurine, glycine and other specific amino acids simulating the intrahepatic microenvironment provide energy and nutritional support for cell proliferation, and promote function maintenance. The efficient and stable in-vitro amplification and function maintenance of the LSEC are realized, and a solid foundation is laid for constructing a high-fidelity LSEC model.

The invention relates to the technical field of microorganisms, and discloses an improved LST broth culture medium and application, and the improved LST broth culture medium comprises tryptone, ammonium chloride, lactose, dipotassium phosphate, monopotassium phosphate, sodium lauryl sulfate, Rpf recombinant protein, magnesium sulfate, sodium pyruvate and pyridoxal phosphate. The improved LST broth culture medium disclosed by the invention can be used for successfully enriching the VBNC-state escherichia coli, so that researchers can successfully detect the VBNC-state escherichia coli in a culture manner.

The application provides a mycoplasma hyopneumoniae culture medium capable of significantly improving the strain titer and an application thereof, and belongs to the field of veterinary biological products. The mycoplasma hyopneumoniae culture medium is obtained by adding L-asparagine, myo-inositol, arachidonic acid, urea, histamine, sodium pyruvate, valproic acid, glyceric acid, lysophosphatidic acid, L-citrulline, L-glutamine and L-leucine into a KM2 culture medium containing Bama pig serum. The culture medium can significantly improve the growth rate and viable bacterial titer of the mycoplasma hyopneumoniae under a low serum concentration, and reduces the production cost.

The invention relates to a culture medium for inducing embryonic stem cells to differentiate into neural stem cells and an induction method. The culture medium is a basic culture medium added with the following concentration components: 1.5 mM to 2.5 mM of L-glutamine substitute, 0.9 * 1.2 * of non-essential amino acid, 0.8 mM to 1.5 mM of sodium pyruvate, 0.1 v / v% to 0.3 v / v% of beta-mercaptoethanol, 0.5 * 1 * of N2 additive, 0.5 * 1 * of B27 additive, 15 ng / mL to 25 ng / mL of bFGF, 15 ng / mL to 25 ng / mL of activin A, 2 mu M to 4 mu M of CHIR99021 and 1 mu M to 3 mu M of XAV939. When the culture medium is used for culturing the embryonic stem cells for a proper time, the embryonic stem cells can be effectively induced to a state which is more favorable for being differentiated into neural stem cells, and then the neural stem cell differentiation induction culture medium is used for continuous culture, so that the efficiency of differentiating the embryonic stem cells into the neural stem cells can be greatly improved; and the obtained neural stem cell expresses specific labeled proteins Nestin and Sox1 and has a garland structure, and the functional phenotype is stable.

The invention discloses a concentrated solution for selective preservation of legionella and application of the concentrated solution, and belongs to the technical field of microbiological detection. The concentrated solution provided by the invention is formed by synergistically combining the following components: a) an HEPES buffer system; b) a selective inhibitor combination comprising glycine, a cephalosporin antibiotic, a glycopeptide antibiotic, polymyxin B and an antifungal agent; c) a legionella physiological activity protective agent composition which comprises sodium pyruvate, betaine and L-cysteine; and d) a storage stabilizer combination comprising trehalose and a chemically modified cyclodextrin. The concentrated solution disclosed by the invention can efficiently and selectively inhibit infectious microbes in sample collection and sample preservation stages, and meanwhile, the physiological activity of legionella is effectively protected.

According to the modification method of the PVA for the high-durability polaroid, boric acid and sodium pyruvate are adopted for modification in the three steps of washing, extending and color fixing, so that the PVA can be better crosslinked, the effect of enhancing the water resistance is achieved, then the modified PVA is applied to the polaroid, the water absorption influence of the PVA in the middle layer can be blocked through the high water resistance, and therefore the water resistance of the polaroid is improved. The water-resistant polaroid glue has good water resistance and durability, can solve the problem of poor water resistance of the existing polaroid glue, improves the durability of the polaroid, realizes that the periphery is not blackened in a double-85 reliability experiment for more than 1000 hours, and is suitable for large-scale industrial production.

The application relates to the field of tumor cell amplification and metabolic regulation, and discloses a novel tumor cell amplification culture medium without animal components and a process, which comprises the following steps: inoculating tumor cells with a culture medium without animal components, monitoring residual glucose concentration in the exponential growth phase, stopping the supply of a basic liquid when the glucose concentration decreases to a trigger threshold interval, single-injecting a sugar-free metabolic reverse pulse liquid containing sodium pyruvate and N-acetyl cysteine into the culture system, maintaining the intervention state, utilizing the concentration gradient formed by the exogenous pyruvate to drive the reverse catalysis of lactate dehydrogenase, inducing the tumor cells to uptake and assimilate lactic acid, and restoring the supply of the sugar-containing basic liquid after the intervention is over. The application establishes an endogenous closed-loop regulation mechanism for metabolic flow, realizes self-assimilation of acidic metabolic waste through endogenous lactic acid removal, avoids self-secretion microenvironment damage caused by physical dilution, and ensures the stability of the genetic characteristics of the cell line.

This invention relates to the field of NK cell culture technology, and discloses an NK culture medium containing taurine and its application in improving NK cell proliferation and killing activity. The medium includes a basal NK cell culture medium and taurine; the basal NK cell culture medium does not contain taurine and includes amino acids, vitamins, inorganic salts, trace elements, D-glucose, and sodium pyruvate. When the basal NK cell culture medium is RPMI_1640 medium, the concentration of taurine is 0.1~1mM; when the basal NK cell culture medium is IMDM medium, the concentration of taurine is 0.4~4mM. This method obtains an NK culture medium by adding taurine to the basal NK cell culture medium. This NK culture medium not only increases the number of NK cells during in vitro culture, but also significantly enhances the killing activity of the cultured NK cells against tumor cells.

The application discloses a method for separating and extracting sodium pyruvate from enzyme conversion liquid for producing sodium pyruvate, which uses enzyme conversion liquid containing sodium pyruvate produced by catalyzing lactic acid with lactic acid oxidase as raw material, removes bacteria bodies, concentrates, sprays, dries and extracts the enzyme conversion liquid with an organic solvent, and comprises the following steps: 1) removing bacteria bodies by filtering the enzyme conversion liquid containing sodium pyruvate through a ceramic membrane; 2) adding activated carbon to perform decolorization treatment; 3) performing concentration by vacuum distillation, so that the content of sodium pyruvate in the enzyme conversion liquid is concentrated to 500-700 g / L; 4) performing spray drying; 5) adding an organic solvent, performing heating, stirring and reflux extraction, cooling to room temperature, centrifugation and drying, and thus pure sodium pyruvate is obtained. The method is simple in operation, low in organic solvent consumption and capable of completely recovering the organic solvent, the produced sodium pyruvate is white in color, the content of the sodium pyruvate is more than 99.6%, the one-time yield is about 96%, the method is low in energy consumption, high in yield and high in product purity, and can be large-scale industrialized production.

The invention discloses a method for measuring the total number of plate bacterial colonies and application of the method. The determination method comprises the following steps: preparing a plate counting culture medium, and carrying out water bath heat preservation on the plate counting culture medium; the plate counting culture medium contains peptone, yeast extract powder, glucose, agar, sodium pyruvate, L-cysteine hydrochloride, magnesium sulfate and hemin; performing gradient dilution on the target bacteria; fully and uniformly mixing the sample homogenate with a plate counting culture medium; covering the surface of the solidified plate counting culture medium with a layer of same plate counting culture medium to form a covering layer; carrying out constant-temperature culture on the solidified flat plates with various dilution degrees; when macroscopic bacterial colonies are formed in the culture medium plate, selecting a plate with 30-300 bacterial colonies on each plate for counting. The method does not need coating operation, can effectively reduce the pollution risk, shortens the detection time, has clear colonial morphology and reliable data, and is suitable for rapid determination of the total number of colonies in various samples.

The application discloses a kit for rapidly detecting bacterial energy metabolism and a preparation method and application thereof, and belongs to the technical field of microorganism analysis, drug screening and detection. The kit comprises reaction premixed powder, anaerobic reaction liquid and optional positive control; the reaction premixed powder comprises the following components in parts by weight: 1 part of benzyl viologen; 0.43-0.64 parts of sodium pyruvate; 0.054-0.082 parts of coenzyme A; 0.037-0.055 parts of MgCl2.7H2O; the anaerobic reaction liquid is a buffer solution containing sodium sulfide-9 hydrate; and the positive control is nitazoxanide or glychalcone A. The application further discloses a preparation method and application of the kit, and the application comprises the following steps: rapidly detecting bacterial energy metabolism activity; and / or screening substances for inhibiting bacterial energy metabolism. The kit can rapidly, sensitively and at low cost evaluate the physiological activity of bacteria, and can also be used for rapidly screening and evaluating anaerobic microorganism energy metabolism inhibitors.

Provided is a transfection reagent, comprising a solvent, organic substances, amino acids, vitamins and inorganic salts, wherein the organic substances comprise D-glucose, sodium pyruvate, i-inositol, phenol red and choline chloride; the amino acids comprise glycine, L-glutamine, L-valine, L-isoleucine, L-leucine, L-threonine, L-lysine hydrochloride, L-phenylalanine, L-serine, L-tyrosine disodium salt, L-arginine hydrochloride, L-methionine, L-cystine dihydrochloride, L-histidine hydrochloride and L-tryptophan; the vitamins comprise nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, folic acid, D-calcium pantothenate and riboflavin; and the inorganic salts comprise sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, sodium dihydrogen phosphate, magnesium sulfate, hydrochloric acid and ferric nitrate. The transfection reagent is suitable for a wide range of applications, achieves the rapid onset of transfection, and has a good transfection effect.

The invention discloses an NK cell culture solution and an NK cell culture method. Comprising a basic culture solution, human serum, recombinant human interleukin-2, recombinant human interleukin-15, recombinant human interleukin-21, recombinant human stem cell factors, a recombinant human FMS-like tyrosine kinase 3 ligand, L-glutamine, beta-mercaptoethanol, non-essential amino acid and sodium pyruvate, wherein the basic culture solution is an IMDM culture solution; the invention relates to the technical field of cell culture. According to the NK cell culture solution and the NK cell culture method, a proper environment is provided for growth, proliferation and activity maintenance of NK cells through reasonable matching of various cell factors, nutritional ingredients and the like, and the culture efficiency of the NK cells can be remarkably improved; meanwhile, all the components in the culture solution have a synergistic effect, the activity and functional stability of the NK cells are effectively maintained, and the culture method is used for culturing the NK cells with the culture solution, operation is easy, and repeatability is high.

The invention discloses a high-efficiency denitrification heterotrophic nitrification-aerobic denitrification bacterium and denitrification application thereof, and relates to the technical field of microbial denitrification. The strain is Achromobacter insolitus H5-1, the preservation number of the Achromobacter insolitus H5-1 is CGMCC (China General Microbiological Culture Collection Center) No.36087, and the 16S rRNA (Ribose Nucleic Acid) gene sequence of the Achromobacter insolitus H5-1 is shown as SEQ ID NO.1 in a sequence table. The strain can be used for synchronous nitrification and denitrification under the aerobic condition, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen can be efficiently degraded, the degradation rate of the ammonia nitrogen within 24 hours reaches 99% or above, the degradation rate of the nitrate nitrogen reaches 85% or above, and nitrite nitrogen accumulation is avoided. The optimal culture conditions are as follows: the carbon source is selected from sodium pyruvate or sodium succinate, the C / N ratio is 15, the pH value is 7.0-8.0, the temperature is 25-35 DEG C, and the rotating speed is 140-180 rpm. The strain can be used for aquaculture tail water, fishery water quality regulation and control and nitrogen-containing sewage treatment.