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This invention discloses a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae, belonging to the field of biotechnology. This invention discovers that compound vitamins and / or bovine liver extract powder as auxiliary components can promote the development of early fourth-stage larvae of Haemonchus contortus into late fourth-stage larvae and adults. This invention provides a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae. The culture medium includes a basal culture medium and auxiliary components, including compound vitamins, bovine liver extract powder, pyruvate, non-essential amino acids, L-glutamine, fetal bovine serum, defibrinated sheep blood, and L-glutathione. The cultivation method includes the step of adding early fourth-stage larvae to the culture medium for cultivation. This invention enables the in vitro culture of adult Haemonchus contortus, which is beneficial for promoting the development of related drugs and vaccines for this parasite and has significant implications for the prevention and control of parasitic diseases.