21 results about "Sheep blood" patented technology

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

This invention discloses a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae, belonging to the field of biotechnology. This invention discovers that compound vitamins and / or bovine liver extract powder as auxiliary components can promote the development of early fourth-stage larvae of Haemonchus contortus into late fourth-stage larvae and adults. This invention provides a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae. The culture medium includes a basal culture medium and auxiliary components, including compound vitamins, bovine liver extract powder, pyruvate, non-essential amino acids, L-glutamine, fetal bovine serum, defibrinated sheep blood, and L-glutathione. The cultivation method includes the step of adding early fourth-stage larvae to the culture medium for cultivation. This invention enables the in vitro culture of adult Haemonchus contortus, which is beneficial for promoting the development of related drugs and vaccines for this parasite and has significant implications for the prevention and control of parasitic diseases.

The invention discloses sheep blood soup and a preparation method thereof, and the preparation method comprises the following steps: adding fresh sheep blood into saline water, then adding flour paste, uniformly mixing, then standing for solidification, and then dicing, boiling and shredding to obtain sheep blood shreds; cooking the sheep bone in water to obtain sheep bone soup; the shredded sheep blood and shredded mutton are added into the sheep bone soup to be boiled, floating foam is skimmed, then seasoning is added, shredded scallions, shredded ginger, minced garlic and sesame oil are added, then coriander is scattered, and the sheep blood soup is obtained. The prepared sheep blood soup is more unique in flavor, richer in layer, more balanced in nutrition and fragrant, thick and smooth in taste, the fishy and mutton smell is effectively removed, and the taste and safety of sheep blood are improved.

The invention relates to the technical field of large animal blood sampling auxiliary instruments, and discloses a turnover type blood sampling device special for sheep blood sampling, which comprises a bottom plate, a supporting mechanism, a turnover frame, a head placing frame, a locking mechanism, a driving mechanism, a steering connecting mechanism, a binding rope and a tightening mechanism. According to the turnover type blood sampling device special for sheep blood sampling, by arranging the turnover frame, the driving mechanism and the steering connecting mechanism, the manual operation intensity is remarkably reduced; in the blood sampling process, a user only needs to fix a sheep on the overturning frame through a binding rope, the steering connecting mechanism is driven to rotate through the driving mechanism and human assistance of a single person, and therefore the overturning frame is driven to automatically reset to the supporting mechanism, and it is not needed that multiple persons lift the sheep or depend on expensive hoisting equipment.

The invention provides an attenuated pertussis CS recombinant bacterium, the CS recombinant bacterium is obtained by mutating a ninth arginine residue of an S1 subunit in a CS strain with a preservation number of CMCC 58003 into a lysine residue and mutating a 129th glutamic acid residue into a glycine residue, and the amino acid sequence of the S1 subunit of the CS recombinant bacterium is as shown in SEQ ID NO.1. The invention further provides a preparation method of the attenuated pertussis CS recombinant bacterium. The method for constructing the recombinant bacteria comprises the following steps: constructing recombinant plasmids; preparing competent cells of the wild pertussis CS strain; electrically transferring the recombinant plasmid into competent cells of the wild pertussis CS strain; respectively preparing BG sheep blood screening plates containing Amp and DOG; sequentially screening a first round and a second round of recombinant pertussis CS strains; and preparing the two-round recombinant pertussis CS strain into a competent strain, and screening twice according to the same steps to obtain the attenuated pertussis CS recombinant strain. The constructed recombinant bacteria can efficiently obtain detoxified PT, and can be applied to products for treating, detecting and preventing pertussis diseases.

The invention provides a helicobacter pylori culture medium, a culture method and application thereof, and relates to the technical field of microbiological culture.The helicobacter pylori culture medium comprises a basic culture medium and antibiotics, and when the basic culture medium is a brain heart infusion agar culture medium, the basic culture medium is a solid culture medium and is used for activation by adding defiberized sheep blood or goat serum; when the basic culture medium is a Brinell broth culture medium, the basic culture medium is a liquid culture medium, essential amino acid is provided by adding L-cysteine, and the basic culture medium is used as a reducing agent, so that enrichment culture of anaerobic bacteria is facilitated. Meanwhile, ferric pyrophosphate, L-cysteine and antibiotics are combined for use, so that rapid growth of the helicobacter pylori is promoted, pollution caused by infectious microbes is effectively reduced, and a more stable growth environment is provided for the helicobacter pylori; data show that when the culture medium is used for culturing helicobacter pylori, compared with a traditional solid culture method, the yield of collected wet thalli is increased by nearly three times, and the high survival rate can be kept while high-speed multiplication culture of helicobacter pylori can be achieved.

This invention provides a molecular marker associated with sheep weight traits and its application. Primers were designed based on the TNNT1 gene sequence to extract DNA from sheep blood. Through PCR amplification, DNA sequencing, and sequence analysis, a G / C polymorphism site was found at 176 bp of the amplified fragment. Further analysis using AQP primers detected the polymorphism site in 918 Hu sheep and established a least-squares model to analyze the association between genotype and weight traits. Ultimately, the TNNT1 gene fragment amplified by this invention was determined to serve as a molecular marker associated with sheep weight traits. By detecting the genotype of this molecular marker, this invention allows for the selection of GG homozygous sheep for breeding in the core herd, which can improve sheep weight and contribute to increased economic benefits.

The application provides PRKAA1 The application provides a gene as a molecular marker related to feed conversion rate of sheep and application thereof, wherein the molecular marker is designed according to PRKAA1 A primer is designed according to the gene sequence, DNA is extracted from sheep blood, and through PCR amplification, DNA sequencing and sequence analysis, a C / T polymorphic site is found at the 165th site of the amplified fragment; further, the polymorphic site of 935 Hu sheep is detected by using KASPar primers, a least square model is established, genotype and feed conversion rate are associated, and finally the application of the amplified PRKAA1 The gene fragment can be used as a molecular marker related to feed conversion rate of sheep. The molecular marker of the application can be used for breeding of high-quality meat sheep with less feed, and provides a genetic engineering means for genetic improvement of feed conversion rate of sheep, and has great practical application value.

The invention discloses a culture medium and a culture method for promoting development of haemonchus contortus larvae, and belongs to the technical field of biology. The compound vitamin and / or the bovine liver extract powder as the auxiliary component can promote the development of the four-stage early larvae of the haemonchus contortus to the four-stage final larvae and adults of the haemonchus contortus. The invention provides a culture medium and a culture method for promoting development of haemonchus contortus larvae, the culture medium comprises a basic culture medium and auxiliary components, the auxiliary components comprise composite vitamins, bovine liver extract powder, pyruvic acid, non-essential amino acids, L-glutamine, fetal calf serum, defibrin sheep blood and L-glutathione; the culture method comprises the step of adding fourth-stage early larvae into the culture medium for culture. According to the method, in-vitro culture of haemonchus contortus adults is achieved, research and development of related drugs and vaccines of parasites are promoted, and the method has important significance on prevention and control of parasitic diseases.

The application provides a molecular marker related to a fat deposition trait of sheep and application thereof. TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP

The invention discloses a histophilic somniphilus, a hemagglutination antigen, a hemagglutination inhibition antibody detection method, a kit and application of the histophilic somniphilus and the hemagglutination antigen. The method comprises the following steps: screening a histophilic sleep bacteria Yunnan isolate ZY25055 with high hemagglutination activity, culturing, and carrying out beta-propiolactone inactivation to prepare the histophilic sleep bacteria hemagglutination antigen. 1% hydroformylated sheep red blood cells are prepared by collecting sheep blood. Immunizing goat posterior jugular vein blood sampling with beta-propiolactone inactivated histophilic sleep bacteria liquid for three times, and centrifugally separating serum to prepare histophilic sleep bacteria standard positive serum. The method and the kit for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody are established by utilizing the prepared histophilic sleeping bacteria hemagglutination antigen, 1% hydroformylated sheep red blood cells and histophilic sleeping bacteria standard positive serum, the method has no cross reaction with positive serum of common pathogens of cattle and sheep, and the method has the advantages of strong specificity, simplicity and convenience in operation, no need of complex equipment and the like, and can be used for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody. The kit can be used for detecting the histophilic somniphilus antibody in bovine serum, sheep serum and goat serum.

The invention discloses an automatic sheep blood sample collecting device, and relates to the technical field of livestock breeding, the automatic sheep blood sample collecting device comprises a blood collecting box and a blood collecting arm, a rotating frame with a mounting groove is arranged in the blood collecting box, and the rotating frame is used for bearing blood collecting tubes; the blood sampling arm comprises a fixing frame and a plurality of hinge frames, the fixing frame is provided with a second circulating belt and a second clamping block, the hinge frames are provided with a first circulating belt and a first clamping block, and the first telescopic rod, the second telescopic rod and the positioning clamp are matched to clamp and move a blood sampling needle; the opening of the top plate corresponds to the blood collection tube and is matched with an infrared positioning sensor and a control system for precise regulation. The device can automatically complete blood taking needle feeding, puncturing, blood taking, sample collecting and continuous operation, manual intervention is not needed, the blood taking efficiency and accuracy are improved, the sample integrity and operation safety are guaranteed, and the device is suitable for large-scale sheep blood collecting scenes.

The application provides a molecular marker related to an immune trait of sheep and application thereof. The molecular marker is designed according to a primer of a GRB10 gene sequence, DNA is extracted from sheep blood, PCR amplification, DNA sequencing and sequence analysis are carried out, a G / A polymorphic site is found at 119bp of the amplified fragment, further, the polymorphic site of 1086 Hu sheep is detected by using an AQP primer, a least square model is established, genotype and immune traits are associated, and finally it is determined that the G / A polymorphic site at 119bp of the amplified GRB10 gene fragment can be used as the molecular marker related to the immune trait of sheep. By detecting the genotype of the molecular marker, the application can select and keep GG homozygous sheep into a core group for breeding, can be used for improving the immune capacity of sheep, and is helpful to increasing economic benefits.

The invention provides a molecular marker related to sheep tail fat deposition and application of the molecular marker in breeding. According to the molecular marker, a primer is designed for an LPL gene sequence, DNA is extracted from sheep blood, PCR amplification, DNA sequencing and sequence analysis are carried out, and it is found that a T / C polymorphic site exists at the 174th site of an amplified fragment shown in SEQ ID NO.1; a KASPar primer is further used for detecting polymorphic sites of 752 sheep and establishing a least square model, correlation analysis is carried out on genotypes and tail fat deposition characters, it is determined that Y at the 174bp position shown in SEQ ID NO.1 is the polymorphic site of T or C, and the polymorphic site can be used as a molecular marker related to sheep tail fat deposition. The molecular marker disclosed by the invention can be used for screening low-tail fat type sheep varieties, can be used for screening assisted breeding and cultivating grain-saving sheep, and is beneficial to increase of economic benefits.

The utility model discloses a portable sheep brucellosis detection device which comprises a detection box, a fixed block is fixed on the back of the detection box, a movable frame is connected in the detection box, and an observation window is embedded in the top of the detection box; and a sliding block is fixed to the bottom of the movable frame, a liquid storage box is installed at the top of the fixed block, a material storage air bag is arranged above the left side in the fixed block, and pipelines are fixed to the upper portions of the left side and the right side of the inner wall of the detection box. According to the portable sheep brucellosis detection device, by means of the arranged detection box, various microorganisms, dust and other impurities in the external environment can be prevented from falling into collected sheep blood or tissue samples, meanwhile, when the movable frame is pushed into the detection box, disinfectant in the material storage air bag can enter the pipeline, and after detection is completed, the disinfectant can enter the pipeline through the pipeline. And the disinfectant is sprayed out through the plurality of spray heads, so that the detection card is disinfected, and the spreading and diffusion of pathogens are avoided.

The application provides a molecular marker related to sheep immunity traits and application thereof. The application uses a method for detecting a PLEKHH2 gene SNP site of Lake sheep by combining DNA pool sequencing with KASPar technology. A primer is designed according to a PLEKHH2 gene sequence, DNA is extracted from sheep blood, and PCR amplification, DNA sequencing and sequence analysis are carried out, so that a C / G polymorphic site at the 368th position of the amplified fragment is found. Further, KASPar primers are used to detect the polymorphic site of 889 Lake sheep and to establish a least square model, and correlation analysis is carried out on the genotype and immunity trait indexes, so that it is found that the site is significantly related to the platelet count (PLT) and the mean corpuscular hemoglobin concentration (MCHC) of sheep. Through detection of the molecular marker of the application, the application can be used in marker-assisted disease-resistant breeding of sheep and can accelerate the breeding process of sheep.

The utility model relates to the technical field of blood filtering and collecting devices, in particular to a blood filtering and collecting device of a cattle and sheep blood drawing operation table, which comprises a support, a filtering piece is fixed on the surface of the support through a fixing piece in a butt joint mode, the filtering piece comprises a puncture needle, and the bottom end of the puncture needle is connected with a drainage tube. The tail end of the drainage pipe is in butt joint with a polyethersulfone membrane filter, the bottom end of the polyethersulfone membrane filter is in butt joint with a hopper cover, the hopper cover is buckled on the surface of a hopper, the bottom end of the hopper is in butt joint with a collecting bag, and a speed adjusting part is arranged at the pipeline end of the polyethersulfone membrane filter. According to the utility model, by pressing the scale strip, the scale strip is controlled to push the pressing plate, so that the pressing plate applies pressure to the pipeline surface of the polyethersulfone membrane filter, and the flow velocity of blood in the pipeline of the polyethersulfone membrane filter is adjusted and changed; therefore, the filtering effect of the polyethersulfone membrane filter is ensured, the filter is prevented from being blocked, blood components are protected, the detection accuracy is ensured, and the detection efficiency is improved to a certain extent.

The invention discloses a sheep blood bean curd and a preparation method thereof, and the preparation method comprises the following steps: adding fresh sheep blood into saline water, then adding flour paste, and uniformly mixing to obtain mixed sheep blood; the mixed sheep blood is subjected to standing solidification and then diced, and sheep blood clots are obtained; and adding the sheep blood clots into boiled water, boiling, and cooling to obtain the sheep blood bean curd. According to the preparation method disclosed by the invention, a unique sheep blood treatment process is adopted, the sheep blood is mixed with the flour paste at normal temperature, and the mixture is solidified, so that the digestion and absorption rate of nutritional ingredients is increased, and the prepared sheep blood bean curd is soft, tender and smooth in mouth feel and small in fishy smell; meanwhile, the process is simpler and more convenient, standardized production is easier, and the raw material manufacturing cost is reduced.

The application provides a molecular marker related to different stage growth traits of sheep and application thereof, the molecular marker is obtained by designing a primer for a XPNPEP2 gene sequence, extracting DNA from sheep blood, amplifying by PCR, DNA sequencing and sequence analysis, finding that a C / T polymorphism site exists at the 95th position of the amplified fragment as shown in SEQ ID NO.1, further detecting the polymorphism site of 740 sheep by using AQP primer, establishing a least square model, and performing correlation analysis on the genotypes and growth traits of chest circumference and pipe circumference, and determining that the Y of the 95bp of SEQ ID NO.1 is a C / T polymorphism site, which can be used as a molecular marker of different stage growth traits of sheep. The molecular marker can be used for screening a new variety of fast growth type high-quality meat sheep, and can be used for screening assisted breeding, cultivating fast growth type sheep, and is helpful to increase economic benefits.

The utility model relates to the technical field of blood treatment, and discloses a defibrating device for sheep blood, which comprises a cylinder body, glass beads are arranged in the cylinder body, a liquid inlet pipe for sheep blood to enter is fixedly mounted at the upper end of the cylinder body, and a liquid outlet pipe for defibrating sheep blood and discharging the defibrated sheep blood is fixedly mounted at the lower end of the right side of the cylinder body. A filter pipe for filtering sheep blood is movably mounted at one end, far away from the barrel, of the liquid outlet pipe, and a liquid extraction pipe is fixedly connected to the upper part of the filter pipe. According to the utility model, the barrel body, the first-stage filter plate, the second-stage filter plate and the liquid pumping pipe are matched, and the first-stage filter plate and the second-stage filter plate are arranged, so that the effect of filtering blood in the liquid outlet pipe again is realized, and the problem of incomplete defibering of the blood in the disclosed technical content is effectively solved; the defibrated blood is primarily filtered by the first-stage filter plate, and the primarily filtered blood is filtered again by the second-stage filter plate, so that the quality of the defibrated blood is improved.