125 results about "Sheep blood" patented technology

The invention relates to the field of livestock and poultry fodder, in particular to special fodder for broilers in a fattening period. The special fodder is made of the following raw materials, by weight, including 180-200 parts of corn, 60-80 parts of wheat bran, 45-50 parts of cornstalks, 35-45 parts of tomato seed meal, 2-3 parts of pepper seed oil, 15-20 parts of sweet potato tubers, 2-3 parts of calcium hydrophosphate, 2-3 parts of English walnut seeds, 2-3 parts of orange peels, 2-3 parts of bean worm powder, 1-2 parts of sheep blood meal, 2-3 parts of shrimp shell meal, 4-5 parts of Doellingeria scaber leaves, 2-3 parts of rhizoma nardostachyos powder, 2-3 parts of helwingia japonica, 2-3 parts of fennel powder, an appropriate amount of table salt and 4-5 parts of phagostimulants. According to the special fodder for the broilers in the fattening period, in combination with the nutritional requirement of the broilers in the fattening period, energy raw materials which can be easily digested and absorbed by the broilers are added into the fodder, so that quick weight increase of the broilers in the fattening period is guaranteed; meanwhile, the other raw materials rich in nutrient content are added, such as the English walnut seeds, the bean worm powder and the rhizoma nardostachyos powder, and as a result, the fodder contains abundant amino acid as well as multiple microelements; the orange peels, the Doellingeria scaber leaves, the helwingia japonica and the like have fragrant smells so that the palatability of the fodder can be improved, and the broilers are in a good feeding state so that the weight of the broilers can be increased quickly.

The invention discloses a method for selecting molecular markers for indicating and identifying sheep wool fiber diameters. The method comprises the following steps of: designing a pair of primers according to a gene sequence KAP 1.1 of sheep; extracting deoxyribonucleic acid (DNA) of a genome from sheep blood, and performing polymerase chain reaction (PCR) amplification on the DNA of the genome of the sheep; performing electrophoretic separation on a PCR product by using polyacrylamide gel, and detecting variant loci inserted/deleted by 60 bp and 30 bp in KAP 1.1 gene exons; and performing polymorphic analysis. The method is easy to operate, low in cost and high in accuracy, and can be used for automated detection. By the method, a simple, effective and convenient molecular marker process is provided for the auxiliary selection of the markers in the sheep breeding process, and an effective molecular marker breeding means is provided for the fineness characters of the sheep, so that the breeding process of high-quality fine-wool sheep can be accelerated.

The invention discloses a fluorescent carbon quantum dot having peroxidase catalytic activity and a preparation method of same. The preparation method includes the following steps: 1) grinding animalblood, (such as hog blood, sheep blood, cattle blood, chicken blood and the like) which are purchased form market, in a mortar into paste, adding deionized water to prepare a turbid liquid, performingultrasonic treatment for 20 min, and adding a less amount of the turbid liquid into a high-pressure reaction kettle to perform a hydrothermal reaction; 2) after the reaction finished, cooling a reaction product to room temperature, filtering the reaction product to remove residues through a filter membrane being of 0.22 [mu]m, and centrifuging a filtrate to remove non-soluble precipitates; 3) performing rotary evaporation to a supernatant to increase the concentration of the carbon quantum dot solution, and freeze-drying the solution to obtain blue fluorescent carbon quantum dots which have strong peroxidase catalytic activity. The synthesized carbon quantum dot powder can be stored for a long time and can be blended according to actual concentration demand when being used. The preparation method has simple operation process and low cost. The carbon quantum dot is high in fluorescence yield, can be directly used in the fields of cell and biological imaging, bio-detection, catalysis, etc.

The invention relates to a bartonia body solid-liquid double-phase slant culture medium as well as a preparation method and an application method thereof. The slant culture medium comprises a slant solid culture medium and a liquid culture medium, wherein the slant solid culture medium comprises beef leach liquid, starch, sodium chloride, agar, defibrinated sheep blood and distilled water; and the liquid culture medium comprises trypticase hydrolysate, soybean protein and papayotin hydrolysate, glucose, sodium chloride, di-potassium phosphate and distilled water. The bartonia body solid-liquid double-phase slant culture medium prepared by the method approximately requires 48 hours to reach 107 CFU/ml, the growth speed is increased by 2.5 times, the cost is saved by 500 times (blood plate culture approximately requires 5 days, and reaching 107 CFU/ml requires 100 flat plates), and the defects of slow bacteria growth, time taking, labor taking, high cost and the like of the traditional bartonia body invitro culture method are remedied .

The invention discloses a sheep blood high-activity immune globulin concentrate and a preparation method thereof, belonging to the field of a food additive. Trisodium citrate is added to sheep blood for centrifugation so as to obtain sheep plasma protein solution; air exhaust is carried out in a closed container so as to obtain the sheep plasma protein solution without sheep odor; the sheep plasma protein solution is diluted by water and is ultra-filtered by a filtering membrane, and then freeze drying is carried out on the sheep plasma protein solution, so as to obtain the sheep blood high-activity immune globulin concentrate. Sheep blood immune globulin has the characteristics of high activity, no sheep odor, good taste and safe using. The preparation method can achieve high yield, and every kilogram of sheep blood can prepare more than 30 kilograms of sheep blood immune globulin concentrate. The preparation method is simple as well as easy and needs low cost; moreover, the energy consumption can be reduced by more than 52%. The invention makes full use of the resource of the sheep blood and reduces environmental pollution.

The invention discloses sheep hemoglobin polypeptide powder and preparation method thereof, belonging to the field of food additives. The method mainly includes the following steps: adding trisodium citrate into sheep blood, then separating the mixture through centrifugation to get sheep hemoglobin solution, putting the sheep hemoglobin solution in a sealed container and exhausting the air in the container through a vacuum pump to remove the mutton odor; diluting the sheep hemoglobin solution through water, adding papain to the solution so as to have enzymatic hydrolysis reaction, and collecting the products of the enzymatic hydrolysis reaction; and ultra-filtering the products through an ultrafiltration membrane, concentrating and drying. In this way, sheep hemoglobin polypeptide powder can be prepared. Firstly, the sheep hemoglobin polypeptide powder prepared through the method is of high nutritional value and has antioxidation, immunoregulation and blood pressure reduction functions; secondly, with no mutton odor, the sheep hemoglobin polypeptide powder is easy to be accepted by consumers; thirdly, the sheep hemoglobin polypeptide powder has high safety and no side effects; fourthly, the preparation method is simple and easy and low in cost; and fifthly, the invention reduces the drainage of sheep blood and environment pollution.

The invention discloses a method for preparing heme of sheep blood, belonging to field of foodstuff and foodstuff additive. It comprises following steps: taking fresh sheep blood as raw material, separating through centrifugation, getting plasma and blood cell, spray drying blood cell into blood cell powder, dissloving with acetone, extracting, stirring, moderating pH, filtering to remove deposition, separating and getting supernatant, drying through distilling and getting heme. The invention is characterized by simple process, reduced equippment investment by 25- 35%, high organic disslovant recovery rate of about 98- 99.9%, low production cost, reduced about by 40- 60%, and high purity heme of about 95- 97%. The quantity of heme extracted from each 1kg blood cell powder is 7.0- 8.5g.

The sperm antibody testing reagent includes sensitized sheep erythrocyte suspension of 2-3 % concentration and rabbit antihuamn IgG in the concentration of 1 to 2 to the (8-10)-th power. The sensitized sheep erythrocyte suspension of 2-3% concentration is prepared through taking sheep blood, washing, hydroformylation, tanning, sensitizing and other steps. The preparation of rabbit antihuman IgG in the concentration of 1 to 2 to the (8-10)-th power includes taking rabbit blood, separating blood serum and other steps. The reagent has strong specificity, high detection sensitivity, no false positive and no false negative phenomenon, high stability, and storage life as long as one year. Using the reagent can obtain easy-to-judge detection result sipmle and fast.

The invention discloses an improved formulation of blood agar culture medium, made from tryptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, defibered sheep blood and agar, wherein each 1000 ml of the culture medium contains 10-20 g of tryptone, 3-8 g of beef extract powder, 1-4 g of liver digest, 0.5-2 g of starch, 3-8 g of yeast powder, 3-8 parts of sodium chloride, 40-60 ml of defibered sheep blood, 10-20 g of agar, and has pH of 7.2+/-0.2. The improved formulation can provide complete nutrition, maintain osmotic pressure for bacteria, and repair the damaged bacteria. The formulation optimized herein may meet the complete demands of common bacteria and most fastidious bacteria, and is good for the cultivation and identification of bacteria.

The invention discloses a negative-pressure sheep slaughter blood collection machine and belongs to auxiliary equipment used in the slaughter process. The negative-pressure sheep slaughter blood collection machine structurally comprises a machine frame, wherein the machine frame is provided with a negative-pressure fan and a collection barrel, the negative-pressure fan and the collection barrel are connected with each other through a negative-pressure pipe, the top end of the collection barrel is provided with a barrel cover, the collection barrel is provided with a collection pipe, one end of the collection pipe is communicated with the collection barrel, the other end of the collection pipe is provided with a collection head, a rotator is arranged at the joint of the collection pipe and the collection barrel, a filter screen is arranged at the joint of the collection pipe and the collection head, the collection pipe is provided with a collection pipe switch, and lifting straps are symmetrically arranged on the two sides of the upper edge of the collection head. The negative-pressure sheep slaughter blood collection machine can collect sheep blood in the slaughter process and also avoids contamination by sundries and microorganisms when a container is open in the collection process.

The invention relates to a luring agent special for luring mandarin fish, which comprises the following components: 1,000g of fresh rough fish, 1,000g of fresh lobster tail, 1,000g of fresh chicken blood, 1,000g of fresh loach, 1,000g of fresh sheep blood, 1,000g of fresh chicken intestine, 500g of silkworm meal, 500g of fish hunger-promoting meal, 500g of fish swelling meal, 300g of fish exhilarant, 300g of fish feed-promoting agent meal, 300g of fish aquatic spice meal and 10,000g of spice albumen powder. The preparation method comprises the following steps: getting the fresh materials according to a certain proportion, respectively spreading and airing; respectively placing into a dryer, controlling the temperature of the dryer to be 65 DEG C, drying for 2h, controlling the dryness of dried materials to be 85 percent; getting out, combining all together, placing into a grinder to be ground into grains of 100 meshes; combining the ground fresh materials with the balance of the materials together after getting out, placing into a mixer to mix for 30min; getting out, placing into the grinder to be ground into powder, and then placing all into a bulking machine to be bulked, controlling the temperature of the bulking machine to be 60 DEG C, bulking for 1-2h, controlling the dryness of the bulked materials to be 75 percent; waiting for cooling after getting out, and then all placing into a pattern hard granulator, producing hard grains, and packaging.

The invention discloses a prevotella intermedia (Pi) culture medium, which is characterized in that: a formula for preparing 1,000 ml of the culture medium contains 15g of peptone, 8g of beef heart infusion, 5 mg of protohemin, 10 mg of vitamin K1, 5.5g of sodium chloride, 15g of agar, 4 mg of doxycycline, 20 mg of metronidazole, 90 ml of sterile sheep blood and the balance being distilled water.Compared with the prior art, the Pi culture medium has the characteristics of reliability in detection and high separation rate of Pi.

The invention provides a blood agar plate, and relates to the technical field of culture media. The blood agar plate contains a plurality of substances such as casein tryptone and soy peptone. The casein tryptone, the soy peptone, yeast extract powder and beef heart infusion provide suitable carbon and nitrogen sources for bacteria; defibrillated sheep blood is rich in nutritional factors to promote the growth of common bacteria, and at the same time the hemolysis of the bacteria can be observed; sodium chloride maintains the osmotic pressure balance of the bacteria; and agar is a culture medium coagulant. The blood agar plate has more comprehensive nutrients, can effectively promote the growth of the bacteria, and improve the isolation rate of the bacteria; according to a preparation method of the blood agar plate, after a culture solution is sterilized at high temperature, cooling is performed to 80-90 DEG C, so that full gas discharging time is given to the culture solution to avoidformation of air bubbles during rapid cooling, and at the same time, before adding the defibrillated sheep blood, full preheating is performed to avoid the problem of agar agglomeration caused by toolarge temperature difference between the defibrillated sheep blood and the culture solution; and the method has simple operation and low requirements for equipment, and is suitable for industrial production.

The invention discloses a disposable selective XV agar culture medium which is characterized in that the culture medium comprises the following components according to the ratios thereof: 3g-5g of beef extract powder, 10g of casein tryptone, 5g of sodium chloride, 15g of agar, 5g of dusty yeast, 15mg-20mg of hemachrome, 15mg-20mg of coenzyme, 9mg of vancocin, and 1,000ml of distilled water or de-ionized water. The culture medium of the invention dispenses with the collection process of sterile defibrinated sheep blood. In the preparation process, well-prepared X and V factors are directly added before pouring, thereby avoiding complex operation of manual shaking in the corpuscle rupture process, greatly reducing preparing time and improving production efficiency.

The present invention relates to a method for specially-catching scale-less fishes, belonging to the field of fishing technology. Said method includes two portions of bait and fishing device. Said bait is made up by using 2000g of small miscellaneous fishes powder, 2000g of lobster powder, 3000g of fresh sheep blood powder, 200g of hungry-promoting powder, 300g of kaempferia root powder, 200g of puffed flour, 500g of adhesive, 300g of aqueous amino acid agent and others as raw material through a certain preparation process. The described fishing device is characterized by that it includes a fishing cage, said fishing cage has a cage body frame, the exterior of said cage body frame is covered with a fishing net, on two opposite end faces of said cage body frame are respectively set a fish inlet and a fish-taking outlet, said fish inlet and fish-taking outlet are barbed holes respectively. Said invention is simple in structure and its fishing efficiency is high.

The invention relates to a strain of strangles streptococcus (also known as streptococcus. equi subsp equi) XZ1 with the preservation number of CGMCC No.7830. The strangles streptococcus strain has the16S rRNA gene sequence shown in the sequence table 1; the strain is isolated from sick horses with strangles and has gram positive bacteria, and cells are spherical and are arranged in pairs or cells are bent chain-shaped, which are in poor growth in ordinary broth and plate; beta type hemolysis happens on a plate with 5% of sheep blood, and a completely transparent hemolytic tape with the width of 3 mm can be formed around small dewdrop-shaped bacterial colonies; facultative anaerobic growth can be realized with the growth temperature from 25 DEG C to 40 DEG C, and the optimum growth temperature is 37 DEG C, and the optimum pH value for growth is between 7.4 and 7.5; glucose is fermented for production of acid, and lactose, mannitol and sorbitol cannot be fermented. The strangles streptococcus strain can be used for preparation of strangles inactivated vaccine, and the prepared strangles inactivated vaccine is good in pertinence, low in cost, safe and good in protection effect.

Meat cat feed comprises: black shrimp powder, chicken liver powder, pig viscera powder, white fish meal, pine needle powder, bean cake powder, sheep liver powder, sheep blood meal, bitter melon protein powder, milk protein powder, corn protein powder, sheep bone powder, compound vitamin B, mineral additives, fructus psoralea powder, salt, licorice powder, hawthorn powder, ginkgo powder, and safflower powder; based on the above scheme, the feed is rich in protein and vitamin; the combination of various nutrient components is reasonable; and the requirements of meat cats for fat, carbohydrates, crude fiber, and mineral matter are all met by the feed; additionally, natural Chinese herbal medicine components such as licorice powder, hawthorn powder, ginkgo powder, and safflower powder are contained in the formula, so the feed has good prevention and treatment effects on cold, cough, snivel, vomit, dehydration, diarrhea, parasite, gastrointestinal diseases and digestive system diseases of meat cats.

A crab bait belongs to fishing baits, which comprises 5,000 grams of lobster powder, 5,000 grams of fresh fish powder, 10,000 grams of fresh sheep blood, 3,000 grams of the powder of flour weevil, 3,000 grams of earthworm powder, 2,000 grams of sea shrimp med, 2,000 grams of raw sheep bone meal, 3,000 grams of animal saprotrophic solution, 1,000 grams of fish-smell fragrance, and 2,000 grams of liquid of silkworm pupa, wherein the preparation method is that the fresh sheep blood is dried in the air, grinded into a fine powder, and mixed with the materials as above in the proper ratios, and then the mixture is put into a pulverization machine with the temperature of 80 DEG C for 30-minute pulverization to obtain the final product of boluses, each of which with a weight of 30 grams is dried in the air for later use. The crab bait has thick fragrance and sweet flavor with a strong fish-smell after being put under water, thereby making the crabs smell the fish-smell even from a long distance and see the baits dispersing and sinking slowly in the water, and luring a great amount of crabs in the shortest period which accumulate together and never leave. The prescribed combination helps lure and capture a great deal of crabs with great quantity with good luring effect and a high capturing rate reaching to 98 percent, and effectively solves the actual puzzle of the difficulty to lure the crabs together which are bred on a complicated terrain.

Bait for fishing the Asian swamp eel comprises, by weight percent, 9-11% of earthworm meal, 6-8% of freshwater shrimp meal, 7-9% of white fish meal, 3-5% of sheep bone meal, 4-6% of chicken meal, 10-12% of sheep liver meal, 4-8% of bean worm meal, 2-6% of blood worm meal, 5-7% of sheep intestine meal, 9-11% of sheep blood meal, 10-12% of sweet potato meal, 1-3% of Chinese liquorice meal, 2-4% of Lysimachia Sikokiana meal, 4-6% of muskmelon meal, 2-4% of banana meal, and 4-6% of pumpkin meal. The expanding bait has good shape and is more convenient for the Asian swamp eel to swallow. The bait similar to live bait is capable of attracting the Asian swamp eels together in a shortest time to grab and eat the bait, and the eels failing to eat the bait tend to swim to and fro in the water in which the bait is put and are unwilling to leave. Therefore, the defects that the traditional bait for the Asian swamp eel is less fragrant, less fishes can be fished with the traditional bait, the Asian swamp eel swallows a hook slowly, and fishing the Asian swamp eel is poorly effective are overcome.

The invention discloses an improved chocolate agar culture medium and a preparation method thereof. The culture medium is prepared by the materials with the following weight percentage: 4 to 5 percent of CM375, 6 to 8 percent of defibering sheep blood, 0.0030 to 0.0050 percent of vancocin and the balance of water. Pharmacological test and clinical trial show that the improved chocolate agar culture medium has abundant nutrient components; the mixture ratio of each component is scientific and reasonable; and strains like hemophilus can grow well in the culture medium and have high survival probability. The improved chocolate agar culture medium brings convenience for clinic microbiological examination and the diagnosis of clinic doctors, and ensures the accuracy of the examination effect. Meanwhile, the materials of the improved chocolate agar culture medium are easily obtained, the preparation method technique of the improved chocolate agar culture medium is simple and the maneuverability is strong; moreover, the invention is suitable for industrial production.

The present invention provides a feed for breeding rice field eels. The feed comprises the following components in weight: 10-15 parts of rapeseed cake meal, 15-30 parts of sheep blood meal, 10-25 parts of pig liver powder, 5-10 parts of oatmeal, 1-15 parts of earthworm powder, 1-20 parts of biogas residues, 10-15 parts of tea saponin, 10-15 parts of bamboo flavonoids, 5-8 parts of tea powder and 3-5 parts of a Chinese herbal medicinal recipe. The sheep blood powder, pig liver powder and earthworm powder are combined with the rapeseed cake meal, the raw materials are used as the main raw materials to prepare the feed, and the feed can provide a large amount of proteins for the growth of the rice field eels. The tea saponin is added into the recipe, so that the feed has the pharmacological functions of preventing against bacteria, preventing viruses, inflammation and oxidation, etc., the added bamboo flavonoids have good immune regulation, and the added tea powder can accelerate the growth of the rice field eels, enable the individual gain weights, and improve the individual disease resistance. By using the recipe to feed the rice field eels, the feed can meet the needs of the markets.

The invention relates to instant fish tofu containing sheep plasma protein and a processing method of the instant fish tofu. The method comprises the following steps: firstly, adding trisodium citrateinto sheep blood, then performing centrifuging, collecting sheep plasma protein obtained by separation, performing freeze-drying to obtain sheep plasma protein powder, taking minced freshwater fish as a raw material, and performing unfreezing, mixing, chopping, heating, forming, frying, sterilizing and cooling to obtain the instant fish tofu. In the invention, the addition amount of the sheep plasma protein powder is 2-3%, the addition amount of iodine-free salt is 2-3%, and konjac glucomannan, curdlan, soy isolate protein, glutamine transferase and the like are added, so that the product hasrelatively high gel strength and water binding capacity, tastes good, and is convenient and instant. The product quality is guaranteed, the requirements of consumers for health, convenience and deliciousness of modern food are met, sheep blood can be fully utilized, instant minced fillet products are enriched, and the market prospect is wide.

The invention relates to a digging bait for luring mandarin fish, which comprises the following components: 600g of small rough fish meal, 600g of lobster meal, 600g of chicken blood meal, 600g of sheep blood meal, 600g of chicken intestine meal, 300g of fish aquatic spice powder, 300g of swelling powder, 300g of atomized powder, 200g of glossy ganoderma powder, 200g of lysimachia sikokiana powder, 500g of powdered milk, 500g of milk albumen powder, 300g of hunger-promoting powder, 300g of binding agent, 500g of spice albumen powder and 10,000g of sweet corn meal. The digging bait is prepared by the following steps: combining the small rough fish meal, the lobster meal, the chicken blood meal, the sheep blood meal and the chicken intestine meal together according to the proportion; putting into a mixer to mix for 60min, getting out, putting into a dryer; controlling the temperature of the dryer to be 50 DEG C, drying for 2h, controlling the dryness of dried materials to be 80 percent, combining the dried materials with the balance materials after getting out, putting into the mixer to mix for 80min, putting into a bulking machine for bulking after getting out, controlling the temperature of the bulking machine to be 80 DEG C, bulking for 150min, controlling the dryness of bulked materials to be 90 percent, waiting for cooling after getting out, putting all into a pattern hard granulation machine to produce hard grains, and packaging normally.

The invention discloses a nocardia asteroid selective medium for a sputum specimen, and belongs to the field of inspective microorganism culture. The nocardia asteroid selective medium is characterized in that 1000ml of the medium comprises beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, argutone, vitamin K1, sterile defibered sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the characteristics of reliable detection and high nocardia asteroid separation rate.