38 results about "Sheep blood" patented technology

The invention discloses sheep blood phosphopeptide chelated iron prepared by a one-pot method, a preparation method and application, and relates to the technical field of preparation methods of peptide chelated iron, the method comprises the following steps: dissolving sheep blood powder in water, and preheating to 50-55 DEG C to obtain a sheep blood powder solution; papain is added into the sheep blood powder solution for enzymolysis, a sheep blood peptide solution is obtained, sodium pyrophosphate is added into the sheep blood peptide solution for phosphorylation modification, and a sheep blood phosphopeptide reaction solution is obtained; adjusting the pH value of the sheep blood phosphopeptide reaction liquid to 3.0-4.0 to dissociate heme groups to obtain dissociation liquid; and centrifuging the dissociation liquid, and recovering the supernatant to obtain the sheep blood phosphopeptide chelated iron. In the preparation process, endogenous iron ions contained in the heme are utilized, so that the complex preparation process of the current blood peptide iron chelate is simplified.

The invention discloses a sheep blood collection and blood routine and biochemical index intelligent detection system and method, and the system comprises a central control processing unit, and a sheep fixing module, an intelligent blood collection module and a blood detection module which are electrically connected with the central control processing unit. After a sheep to be subjected to blood sampling is mechanically limited and flexibly restrained through the sheep fixing module, the central control processing unit is used for controlling the intelligent blood sampling module to carry out individualized blood sampling, and after blood sampling is completed, the blood detection module is used for carrying out multi-index detection on the collected blood. According to the invention, through a mode of combining the flexible pneumatic air bag and mechanical limitation, accurate and mild constraint is applied to the sheep, automatic and low-stress acquisition of sheep blood is realized, blood routine and biochemical indexes are rapidly detected in combination with multi-parameter analysis, the detection efficiency and data accuracy are improved, and the detection cost is reduced. On-site instant technical support is provided for sheep health monitoring, and the health management level of a large-scale farm is improved.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The invention discloses a transplanted tumor containing helicobacter pylori and a preparation method and application thereof, belongs to the technical field of biology, and further discloses a reagent for preparing a transplanted tumor tissue containing helicobacter pylori, and the reagent comprises tumor cells and helicobacter pylori and further comprises at least one of matrigel and defibrated sheep blood. The helicobacter pylori-containing transplanted tumor obtained by utilizing the reagent and the preparation method disclosed by the invention can be used for preparing a quality control substance or a reference substance in immunological detection, the HP thallus positive staining is stronger, and besides being used as positive quality control, the helicobacter pylori-containing transplanted tumor also has internal control of negative cell hematoxylin staining, so that the helicobacter pylori-containing transplanted tumor can be used for preparing the quality control substance or the reference substance. The same quality control product has both thallus positive staining and histocyte negative staining, has consistency with conventional case tissue detection, and can play a monitoring role in HP immunohistochemical quality control.

The application provides a molecular marker related to sheep growth traits and application thereof, the molecular marker is designed by primers according to the sequence of the LGALS3 gene, DNA is extracted from sheep blood, and through PCR amplification, DNA sequencing and sequence analysis, it is found that a T / C polymorphism site exists at the 298th position of the amplified fragment, further, the polymorphism site of 849 Hu sheep is detected by using AQP primers, a least square model is established, genotype and growth traits are associated, finally, it is determined that Y at the 298th bp of the amplified LGALS3 gene fragment shown in SEQ ID NO. 1 is T or C, the mutation leads to T / C polymorphism of the molecular marker, and the molecular marker can be used as a molecular marker related to sheep growth traits. The molecular marker of the application can be used for screening a new variety of fast-growing high-quality mutton sheep, and can be used for screening assisted breeding, cultivating fast-growing sheep, and helping to increase economic benefits.

The present invention provides a molecular marker associated with resistance to Mycoplasma pneumonia lesions in sheep and its application. The molecular marker was screened from the FOXF1 gene in a GWAS of lung tissue pathology scores. DNA was extracted from sheep blood, and the polymorphic sites of 203 Hu sheep were detected using KASPar primers. A least squares model was established, and the genotypes of the polymorphic sites in the 203 Hu sheep were associated with the pneumonia lesion phenotypic data. Ultimately, it was determined that the FOXF1 gene and its related SNPs screened by the present invention can be used as molecular markers associated with resistance to Mycoplasma pneumonia lesions in sheep. The molecular markers associated with resistance to Mycoplasma pneumonia lesions provided by the present invention can be used to screen high-quality meat sheep with high resistance to Mycoplasma pneumonia, providing a genetic engineering method for rapid screening of disease-resistant sheep breeding, and having significant practical application value.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

The present invention relates to a single nucleotide polymorphism detection primer for the sheep BMPR1B gene and its application, belonging to the field of gene technology. The SNP molecular marker corresponding to the primer of the present invention is located on chromosome 6 of the BMPR1B gene, and the primer is used for sheep marker-assisted selection breeding. The present invention provides a plurality of SNP molecular marker sites related to sheep reproductive traits, and determines the genotype of each site by directly sequencing the PCR product using sheep blood genomic DNA as a template. The present invention can be used as an effective molecular marker to improve sheep reproductive traits. The detection method is simple, rapid, and highly accurate, which can improve the breeding efficiency of sheep. It has high application value and provides more ways for the screening research of sheep reproductive traits.

This invention discloses a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae, belonging to the field of biotechnology. This invention discovers that compound vitamins and / or bovine liver extract powder as auxiliary components can promote the development of early fourth-stage larvae of Haemonchus contortus into late fourth-stage larvae and adults. This invention provides a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae. The culture medium includes a basal culture medium and auxiliary components, including compound vitamins, bovine liver extract powder, pyruvate, non-essential amino acids, L-glutamine, fetal bovine serum, defibrinated sheep blood, and L-glutathione. The cultivation method includes the step of adding early fourth-stage larvae to the culture medium for cultivation. This invention enables the in vitro culture of adult Haemonchus contortus, which is beneficial for promoting the development of related drugs and vaccines for this parasite and has significant implications for the prevention and control of parasitic diseases.

The invention discloses sheep blood soup and a preparation method thereof, and the preparation method comprises the following steps: adding fresh sheep blood into saline water, then adding flour paste, uniformly mixing, then standing for solidification, and then dicing, boiling and shredding to obtain sheep blood shreds; cooking the sheep bone in water to obtain sheep bone soup; the shredded sheep blood and shredded mutton are added into the sheep bone soup to be boiled, floating foam is skimmed, then seasoning is added, shredded scallions, shredded ginger, minced garlic and sesame oil are added, then coriander is scattered, and the sheep blood soup is obtained. The prepared sheep blood soup is more unique in flavor, richer in layer, more balanced in nutrition and fragrant, thick and smooth in taste, the fishy and mutton smell is effectively removed, and the taste and safety of sheep blood are improved.

The invention relates to the technical field of large animal blood sampling auxiliary instruments, and discloses a turnover type blood sampling device special for sheep blood sampling, which comprises a bottom plate, a supporting mechanism, a turnover frame, a head placing frame, a locking mechanism, a driving mechanism, a steering connecting mechanism, a binding rope and a tightening mechanism. According to the turnover type blood sampling device special for sheep blood sampling, by arranging the turnover frame, the driving mechanism and the steering connecting mechanism, the manual operation intensity is remarkably reduced; in the blood sampling process, a user only needs to fix a sheep on the overturning frame through a binding rope, the steering connecting mechanism is driven to rotate through the driving mechanism and human assistance of a single person, and therefore the overturning frame is driven to automatically reset to the supporting mechanism, and it is not needed that multiple persons lift the sheep or depend on expensive hoisting equipment.

The invention relates to a sheep BMPR1B gene single nucleotide polymorphism detection primer and application, and belongs to the technical field of genes. The SNP molecular marker corresponding to the primer is located on the sixth chromosome of the BMPR1B gene, and the primer is used for marker-assisted selective breeding of sheep. The invention provides a plurality of SNP molecular marker sites related to sheep breeding traits, and the genotype of each site is judged by a method of taking sheep blood genome DNA as a template and directly sequencing a PCR product. The molecular marker can be used as an effective molecular marker for improving the breeding traits of the sheep, the detection method is simple and rapid, the accuracy is high, the breeding efficiency of the sheep can be improved, the application value is very high, and more ways are provided for screening and researching the breeding traits of the sheep.

The invention provides an attenuated pertussis CS recombinant bacterium, the CS recombinant bacterium is obtained by mutating a ninth arginine residue of an S1 subunit in a CS strain with a preservation number of CMCC 58003 into a lysine residue and mutating a 129th glutamic acid residue into a glycine residue, and the amino acid sequence of the S1 subunit of the CS recombinant bacterium is as shown in SEQ ID NO.1. The invention further provides a preparation method of the attenuated pertussis CS recombinant bacterium. The method for constructing the recombinant bacteria comprises the following steps: constructing recombinant plasmids; preparing competent cells of the wild pertussis CS strain; electrically transferring the recombinant plasmid into competent cells of the wild pertussis CS strain; respectively preparing BG sheep blood screening plates containing Amp and DOG; sequentially screening a first round and a second round of recombinant pertussis CS strains; and preparing the two-round recombinant pertussis CS strain into a competent strain, and screening twice according to the same steps to obtain the attenuated pertussis CS recombinant strain. The constructed recombinant bacteria can efficiently obtain detoxified PT, and can be applied to products for treating, detecting and preventing pertussis diseases.

The invention provides a helicobacter pylori culture medium, a culture method and application thereof, and relates to the technical field of microbiological culture.The helicobacter pylori culture medium comprises a basic culture medium and antibiotics, and when the basic culture medium is a brain heart infusion agar culture medium, the basic culture medium is a solid culture medium and is used for activation by adding defiberized sheep blood or goat serum; when the basic culture medium is a Brinell broth culture medium, the basic culture medium is a liquid culture medium, essential amino acid is provided by adding L-cysteine, and the basic culture medium is used as a reducing agent, so that enrichment culture of anaerobic bacteria is facilitated. Meanwhile, ferric pyrophosphate, L-cysteine and antibiotics are combined for use, so that rapid growth of the helicobacter pylori is promoted, pollution caused by infectious microbes is effectively reduced, and a more stable growth environment is provided for the helicobacter pylori; data show that when the culture medium is used for culturing helicobacter pylori, compared with a traditional solid culture method, the yield of collected wet thalli is increased by nearly three times, and the high survival rate can be kept while high-speed multiplication culture of helicobacter pylori can be achieved.

This invention provides a molecular marker associated with sheep weight traits and its application. Primers were designed based on the TNNT1 gene sequence to extract DNA from sheep blood. Through PCR amplification, DNA sequencing, and sequence analysis, a G / C polymorphism site was found at 176 bp of the amplified fragment. Further analysis using AQP primers detected the polymorphism site in 918 Hu sheep and established a least-squares model to analyze the association between genotype and weight traits. Ultimately, the TNNT1 gene fragment amplified by this invention was determined to serve as a molecular marker associated with sheep weight traits. By detecting the genotype of this molecular marker, this invention allows for the selection of GG homozygous sheep for breeding in the core herd, which can improve sheep weight and contribute to increased economic benefits.

The application provides PRKAA1 The application provides a gene as a molecular marker related to feed conversion rate of sheep and application thereof, wherein the molecular marker is designed according to PRKAA1 A primer is designed according to the gene sequence, DNA is extracted from sheep blood, and through PCR amplification, DNA sequencing and sequence analysis, a C / T polymorphic site is found at the 165th site of the amplified fragment; further, the polymorphic site of 935 Hu sheep is detected by using KASPar primers, a least square model is established, genotype and feed conversion rate are associated, and finally the application of the amplified PRKAA1 The gene fragment can be used as a molecular marker related to feed conversion rate of sheep. The molecular marker of the application can be used for breeding of high-quality meat sheep with less feed, and provides a genetic engineering means for genetic improvement of feed conversion rate of sheep, and has great practical application value.

The present invention provides a method for rapidly separating ovine blood albumin based on an aqueous two-phase system, and the aqueous two-phase system includes ethanol, sodium dihydrogen phosphate and water. By using an aqueous two-phase system with specific components and ratios screened and optimized, protein aggregation or denaturation can be effectively prevented, the quality of ovine blood albumin is guaranteed to a great extent, the recovery rate and purity of albumin are significantly improved, and the utilization rate of ovine blood is greatly increased. With these advantages, this method and the ovine blood albumin extracted by using this method show broad application prospects in many fields such as food and biomaterials.

The invention discloses a culture medium and a culture method for promoting development of haemonchus contortus larvae, and belongs to the technical field of biology. The compound vitamin and / or the bovine liver extract powder as the auxiliary component can promote the development of the four-stage early larvae of the haemonchus contortus to the four-stage final larvae and adults of the haemonchus contortus. The invention provides a culture medium and a culture method for promoting development of haemonchus contortus larvae, the culture medium comprises a basic culture medium and auxiliary components, the auxiliary components comprise composite vitamins, bovine liver extract powder, pyruvic acid, non-essential amino acids, L-glutamine, fetal calf serum, defibrin sheep blood and L-glutathione; the culture method comprises the step of adding fourth-stage early larvae into the culture medium for culture. According to the method, in-vitro culture of haemonchus contortus adults is achieved, research and development of related drugs and vaccines of parasites are promoted, and the method has important significance on prevention and control of parasitic diseases.

The application provides a molecular marker related to a fat deposition trait of sheep and application thereof. TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP TM The application is characterized in that the molecular marker is designed according to a primer of an ABCC3 gene sequence, DNA is extracted from sheep blood, and a A / T polymorphic site is found at the 354th bp of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, and the AQP

The invention discloses a histophilic somniphilus, a hemagglutination antigen, a hemagglutination inhibition antibody detection method, a kit and application of the histophilic somniphilus and the hemagglutination antigen. The method comprises the following steps: screening a histophilic sleep bacteria Yunnan isolate ZY25055 with high hemagglutination activity, culturing, and carrying out beta-propiolactone inactivation to prepare the histophilic sleep bacteria hemagglutination antigen. 1% hydroformylated sheep red blood cells are prepared by collecting sheep blood. Immunizing goat posterior jugular vein blood sampling with beta-propiolactone inactivated histophilic sleep bacteria liquid for three times, and centrifugally separating serum to prepare histophilic sleep bacteria standard positive serum. The method and the kit for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody are established by utilizing the prepared histophilic sleeping bacteria hemagglutination antigen, 1% hydroformylated sheep red blood cells and histophilic sleeping bacteria standard positive serum, the method has no cross reaction with positive serum of common pathogens of cattle and sheep, and the method has the advantages of strong specificity, simplicity and convenience in operation, no need of complex equipment and the like, and can be used for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody. The kit can be used for detecting the histophilic somniphilus antibody in bovine serum, sheep serum and goat serum.

The invention provides a molecular marker related to sheep feed conversion rate and application thereof, the molecular marker is obtained by designing a primer for an FABP5 gene sequence, extracting DNA (deoxyribonucleic acid) from sheep blood, performing PCR (polymerase chain reaction) amplification, DNA sequencing and sequence analysis, and finding that a C / G polymorphic site exists at the 2063rd site of an amplified fragment as shown in SEQ ID NO.1, and the C / G polymorphic site exists at the 2063rd site of the amplified fragment as shown in SEQ ID NO.2. An AQP primer is further used for detecting polymorphic sites of 791 sheep and establishing a least square model, correlation analysis is performed on genotypes and growth character chest circumference and tube circumference, and the polymorphic site with S at the 2063bp as shown in SEQ ID NO.1 being C or G is determined and can be used as a molecular marker related to the feed conversion rate of the sheep. The molecular marker provided by the invention can be used for screening new varieties of high-quality mutton sheep with high feed efficiency, can be used for screening assisted breeding and breeding grain-saving sheep, and is beneficial to increase of economic benefits.

The invention discloses an automatic sheep blood sample collecting device, and relates to the technical field of livestock breeding, the automatic sheep blood sample collecting device comprises a blood collecting box and a blood collecting arm, a rotating frame with a mounting groove is arranged in the blood collecting box, and the rotating frame is used for bearing blood collecting tubes; the blood sampling arm comprises a fixing frame and a plurality of hinge frames, the fixing frame is provided with a second circulating belt and a second clamping block, the hinge frames are provided with a first circulating belt and a first clamping block, and the first telescopic rod, the second telescopic rod and the positioning clamp are matched to clamp and move a blood sampling needle; the opening of the top plate corresponds to the blood collection tube and is matched with an infrared positioning sensor and a control system for precise regulation. The device can automatically complete blood taking needle feeding, puncturing, blood taking, sample collecting and continuous operation, manual intervention is not needed, the blood taking efficiency and accuracy are improved, the sample integrity and operation safety are guaranteed, and the device is suitable for large-scale sheep blood collecting scenes.

The application provides a molecular marker related to an immune trait of sheep and application thereof. The molecular marker is designed according to a primer of a GRB10 gene sequence, DNA is extracted from sheep blood, PCR amplification, DNA sequencing and sequence analysis are carried out, a G / A polymorphic site is found at 119bp of the amplified fragment, further, the polymorphic site of 1086 Hu sheep is detected by using an AQP primer, a least square model is established, genotype and immune traits are associated, and finally it is determined that the G / A polymorphic site at 119bp of the amplified GRB10 gene fragment can be used as the molecular marker related to the immune trait of sheep. By detecting the genotype of the molecular marker, the application can select and keep GG homozygous sheep into a core group for breeding, can be used for improving the immune capacity of sheep, and is helpful to increasing economic benefits.

The invention relates to a mutton smell removing agent, mutton smell-free quick-frozen fresh sheep blood and a preparation method thereof, and relates to the technical field of quick-frozen fresh sheep blood, the mutton smell removing agent comprises the following components by weight: 0.1%-0.5% of modified soybean phospholipid, 1%-5% of glucose, 0.01%-0.05% of sodium carbonate, 0.1%-0.3% of a rosemary extract, 1%-5% of sodium erythorbate, 0.1%-0.5% of guar gum, and the balance of water. 0.01%-0.06% of ethyl maltol and 1%-5% of spice juice; and 83%-97% of water. The mutton smell removing agent provided by the invention can be applied to preparation of quick-frozen fresh sheep blood, can effectively remove the mutton smell of the sheep blood in a low-temperature state, ensures the palatability of the sheep blood, not only can effectively remove the mutton smell, but also can maintain the nutritional ingredients and taste of the sheep blood, and makes up for the defects in the prior art.

The invention provides a molecular marker related to growth traits of sheep at different stages and application of the molecular marker. The molecular marker is characterized in that a primer is designed for an XPNPEP2 gene sequence, DNA is extracted from sheep blood, and a C / T polymorphic site exists at the 95th site of an amplified fragment, such as SEQ ID NO.1, through PCR amplification, DNA sequencing and sequence analysis; an AQP primer is further used for detecting polymorphic sites of 740 sheep and establishing a least square model, correlation analysis is carried out on genotypes and chest circumference and tube circumference of growth traits, and it is determined that Y at the 95bp position as shown in SEQ ID NO.1 is C or T polymorphic sites, and the polymorphic sites can be used as molecular markers of the growth traits of the sheep at different stages. The molecular marker disclosed by the invention can be used for screening new varieties of rapid growth type high-quality mutton sheep, can be used for screening assisted breeding and breeding rapid growth type sheep, and is beneficial to increase of economic benefits.

The invention provides a molecular marker related to sheep tail fat deposition and application of the molecular marker in breeding. According to the molecular marker, a primer is designed for an LPL gene sequence, DNA is extracted from sheep blood, PCR amplification, DNA sequencing and sequence analysis are carried out, and it is found that a T / C polymorphic site exists at the 174th site of an amplified fragment shown in SEQ ID NO.1; a KASPar primer is further used for detecting polymorphic sites of 752 sheep and establishing a least square model, correlation analysis is carried out on genotypes and tail fat deposition characters, it is determined that Y at the 174bp position shown in SEQ ID NO.1 is the polymorphic site of T or C, and the polymorphic site can be used as a molecular marker related to sheep tail fat deposition. The molecular marker disclosed by the invention can be used for screening low-tail fat type sheep varieties, can be used for screening assisted breeding and cultivating grain-saving sheep, and is beneficial to increase of economic benefits.

The utility model discloses a portable sheep brucellosis detection device which comprises a detection box, a fixed block is fixed on the back of the detection box, a movable frame is connected in the detection box, and an observation window is embedded in the top of the detection box; and a sliding block is fixed to the bottom of the movable frame, a liquid storage box is installed at the top of the fixed block, a material storage air bag is arranged above the left side in the fixed block, and pipelines are fixed to the upper portions of the left side and the right side of the inner wall of the detection box. According to the portable sheep brucellosis detection device, by means of the arranged detection box, various microorganisms, dust and other impurities in the external environment can be prevented from falling into collected sheep blood or tissue samples, meanwhile, when the movable frame is pushed into the detection box, disinfectant in the material storage air bag can enter the pipeline, and after detection is completed, the disinfectant can enter the pipeline through the pipeline. And the disinfectant is sprayed out through the plurality of spray heads, so that the detection card is disinfected, and the spreading and diffusion of pathogens are avoided.

The invention belongs to the technical field of animal models, and particularly relates to a preparation method for constructing a rat embryo implantation failure model based on streptococcus agalactiae. The method comprises the following steps: culturing a streptococcus agalactiae strain with the number of BNCC336970 by using a solid culture medium containing sheep blood, adjusting the concentration of a bacterial solution to 1 * 10 < 8 > CFU / mL, and carrying out vaginal perfusion on an SPF-grade female SD rat every day for 21 days continuously. And after the perfusion is finished, the female mouse is mated with the male mouse at the early oestrus stage, the detected vaginal suppository is marked as the 0.5 th day of pregnancy, the mouse is killed at the 7.5 th day of pregnancy, and the model is evaluated by counting the embryo implantation quantity and detecting the expression level of endometrial tissue receptivity factors MMP9 and VEGF (Vascular Endothelial Growth Factor). A clinical pathogen pathogenic mechanism is simulated through a genital tract infection way for the first time, and the problem of physiological status deviation caused by traditional hormone intervention, immune agonists or genetic engineering methods is solved. The constructed model can stably induce the obvious reduction of the embryo implantation number and the reduction of endometrial receptivity, and provides a standardized tool for the pathological mechanism research of infectious embryo implantation failure and the screening of therapeutic drugs.

The invention provides a molecular marker related to sheep mycoplasmal pneumonia lesion resistance and application of the molecular marker. The molecular marker is screened from an FOXF1 gene in lung tissue pathological score GWAS, DNA is extracted from sheep blood, a KASPar primer is used for detecting polymorphic sites of 203 Hu sheep, a least square model is established, correlation analysis is performed on genotypes of the polymorphic sites of the 203 Hu sheep and pneumonia lesion phenotype data, and the genotypes of the polymorphic sites of the 203 Hu sheep and pneumonia lesion phenotype data are analyzed. Finally, it is determined that the FOXF1 gene screened in the invention and the related SNP thereof can be used as the molecular marker related to the sheep mycoplasma pneumonia disease resistance. The molecular marker related to the mycoplasmal pneumonia disease resistance of sheep can be used for screening high-quality mutton sheep with high mycoplasmal pneumonia resistance, provides a genetic engineering means for rapid screening of sheep disease resistance breeding, and has great practical application value.

The invention belongs to the technical field of screening and preparation of sheep molecular markers, and relates to a molecular marker related to sheep feed conversion rate and application thereof. The molecular marker is characterized in that a primer is designed according to an FABP7 gene sequence, DNA is extracted from sheep blood, an A / G polymorphic site exists at the 255th site of an amplified fragment through PCR amplification, DNA sequencing and sequence analysis, an AQPTM primer is further used for detecting the polymorphic sites of 821 Hu sheep and establishing a least square model, and the FABP7 gene sequence is used for detecting the polymorphic sites of the 821 Hu sheep. The genotype and the feed conversion rate are subjected to correlation analysis, and finally it is determined that the amplified FABP7 gene segment can serve as the molecular marker related to the sheep feed conversion rate. The molecular marker disclosed by the invention can be used for breeding a new variety of grain-saving high-quality mutton sheep, provides a genetic engineering means for genetic improvement of the feed conversion rate of sheep, and has an important practical application value.