4901results about "Animal husbandry" patented technology

The invention provides a laying hen feeding scheme adjustment method and system based on moulting image recognition, and relates to the technical field of intelligent breeding, and the method comprises the steps: obtaining a multi-angle body surface image sequence synchronously collected by a plurality of laying hen individuals in a target chicken flock under a natural light condition; performing semantic segmentation according to the multi-angle body surface image sequence to obtain a feather contour segmentation map set; performing group feature joint extraction according to the feather contour segmentation map set to obtain a multi-dimensional feature tensor representing individual features and group distribution information at the same time; performing group state collaborative inference according to the multi-dimensional feature tensor to obtain a three-dimensional probability distribution vector; performing group nutrition demand mapping according to the three-dimensional probability distribution vector to obtain an optimal nutrition parameter combination suitable for the whole chicken flock; and generating a feeding scheme according to the optimal nutrition parameter combination to obtain a feeding instruction stream. The accuracy and adaptability of nutrition regulation and control in the moulting period are effectively improved.

Compounds having the structure of Formula (I): or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof; wherein R1, R2, R3, R4, R5, L1, L2, a and z are as defined herein, are disclosed. Use of these compounds as a component of lipid nanoparticle formulations for delivery of a therapeutic agent, compositions comprising the compounds, and methods for their use and preparation are also provided.

Disclosed in the present invention are an HLA-A*11-targeted liver cancer mRNA vaccine, and a preparation method therefor and a use thereof. The mRNA vaccine of the present invention is an mRNA vaccine designed on the basis of the HLA-A*11:01 typing of a patient and having high-coverage and high-immunogenic tumor neoantigens, and is formed by transcribing DNA having a nucleotide sequence shown in SEQ ID NO: 32 to form an mRNA, and encapsulating the mRNA in lipid nanoparticles.

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.

The utility model relates to the technical field of aquaculture, and particularly discloses a movable Chinese softshell turtle floating spawning site which comprises a bottom plate, a spawning room frame structure is detachably arranged on the bottom plate, and a spawning room is formed in the space between the spawning room frame structure and the bottom plate. A floating cavity and a buoyancy piece are arranged at the bottom of the bottom plate, a positioning assembly is arranged at the bottom of the floating cavity, and a fixing anchor assembly is arranged at the bottom of the positioning assembly. A frame-shaped inlet and a frame-shaped outlet are formed in the opposite sides of the spawning room frame structure respectively; a house entering ladder is arranged at the frame-shaped inlet; a house outlet ladder is arranged at the frame-shaped outlet, and the surface of the house outlet ladder is a smooth inclined surface; a lifting structure is arranged on the oviposition room frame structure, a plurality of single-layer oviposition plates are detachably arranged on the lifting structure, oviposition cavities are formed in the single-layer oviposition plates, and landslide sections for guiding the oviposition cavities are arranged on the single-layer oviposition plates; a climbing section for guiding the oviposition cavity is arranged on the single-layer oviposition plate. The device has the advantages of being movable in arrangement mode, good in flexibility and low in moving cost.

The invention belongs to the technical field of biotechnology and animal models, and discloses a double-person-derived mouse model for simulating a tumor immune microenvironment and a construction method and application thereof. The method comprises the following steps: firstly, pretreating NSG immunodeficient mice by adopting low-dose whole-body irradiation in combination with double-antibody targeted bone marrow depletion, and transplanting CD34 + hematopoietic stem cells from the same human donor to complete human immune system reconstruction; separating tumor primary cells, tumor-related fibroblasts and tumor vascular endothelial cells of the same donor, performing three-dimensional co-culture to obtain homologous human tumor organs, and performing in-situ inoculation to immune reconstruction mice to obtain a target model. The core defects of MHC mismatching, low immune reconstruction efficiency, poor tumor immune microenvironment simulation degree, low clinical consistency and the like of an existing model are overcome, and the method can be used for tumor immune treatment drug screening, microenvironment mechanism research and personalized tumor treatment scheme verification.

The invention provides an application of WNT7B in construction of a myopia animal model. Homologous genes wnt7ba and wnt7bb of the WNT7B in the zebra fish are knocked out or knocked down by applying a CRISPR / Cas9 gene editing technology, an animal model of which the eye axis length is remarkably increased, the eyeball movement frequency is remarkably reduced and the light response is reduced is obtained, and the result shows that the WNT7B is related to the high myopia. The model provides a favorable tool for pathogenesis and drug screening of high myopia, and has a good application prospect.

Aspects of the disclosure relate to complexes comprising an anti-TfR1 antibody covalently linked (e.g., via a linker such as a peptide linker) to a lysosomal enzyme (e.g., an acid alpha glucosidase enzyme), and methods of making and using the fusion complexes to treat a lysosomal storage disease (e.g., Pompe disease).

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention discloses a Parkinson's disease animal model, a construction method and application, and relates to the field of animal genetic engineering and disease model construction. According to the model, seven mutation sites related to the human familial Parkinson's disease are introduced behind an initiation codon of a fourth exon of an endogenous SNCA gene, and the mutation sites are A18T, A29S, A30P, E46K, H50Q, G51D and A53T and have genotypes related to the human familial Parkinson's disease. The pig model shows nigra dopaminergic neuron reduction and cortical neuron reduction in the newborn period, and the core pathological process of the human Parkinson's disease can be systematically reproduced. The model can be widely applied to Parkinson's disease pathogenesis research, drug screening and cell therapy effect evaluation, and has important scientific research and preclinical application values.

The invention discloses a humanized osteoporosis mouse model and a construction method thereof, and belongs to the technical field of biotechnology and animal models. The invention aims at the core defects of large species difference, insufficient humanization degree, low pathological simulation degree, unstable phenotype and the like of the existing osteoporosis model. The humanized osteoporosis mouse model is constructed through five core steps of experimental animal pretreatment, collagen-nano hydroxyapatite bionic scaffold preparation and cell pre-implantation, immune-bone metabolism double-derived system construction, progressive osteoporosis induction and model identification and verification. According to the method, double-person origination of immune and bone metabolism systems is realized for the first time, the colonization rate and survival time of human cells are greatly improved, the pathological process of clinical postmenopausal osteoporosis is accurately simulated, the phenotype of the model is stable and uniform, the reactivity matching degree with clinical drugs is high, and the method can be widely applied to research and development of anti-osteoporosis drugs and research of pathological mechanisms.

The invention relates to the technical field of intelligent agriculture, and discloses a laying hen antibiotic-free breeding system and method.The technical defect that a general model cannot adapt to the influences of biological individual heterogeneity and environment hysteresis is overcome by constructing a dynamic metabolism reference containing environment correction logic, dynamic and accurate adaptation of the breeding reference is achieved, and meanwhile, the breeding efficiency of laying hens is improved. By means of parallel time sequence feature extraction and metabolic damping kinetic analysis, medicine forced intervention signals and natural physiological recovery signals are effectively separated and recognized on the physical level, the problem that hidden illegal medicine use is difficult to monitor is solved, in addition, in combination with an antagonism verification mechanism of the law of conservation of biological energy, the accuracy of monitoring is improved. A non-violating physical anti-counterfeiting barrier is constructed, the data modification behavior is effectively identified, and the supervision transparency and compliance credibility of the whole process of antibiotic-free cultivation are remarkably improved.

The invention relates to the field of disease animal model construction, and particularly discloses a postmenopausal osteoporosis and knee osteoarthritis co-disease animal model construction method which comprises the following steps: selecting a female animal as an experimental subject; the selected female animals are injected with a GnRH agonist, meanwhile, low-calcium feed feeding and oxidative stress stimulation are conducted, and feeding is conducted for a preset time; detecting bone mineral density and bone trabecula parameters of the fed female animals, and screening qualified female animals meeting set standards; performing meniscus tearing operation on the qualified female animal, and implanting sustained release microspheres loaded with inflammatory factors at the torn part during the operation; carrying out weight-bearing training on the female animal after the operation, and continuing for a set time length; and performing health detection on the female animals at different time points. According to the method, through multi-factor collaborative induction of the PMOP, minimally invasive construction of the KOA and multi-dimensional health detection, accurate simulation of a co-disease pathological process and clinical characteristics is realized, and the authenticity and stability of the model are improved.

The invention discloses a hog house environment regulation and control method based on AI visual analysis, and relates to the technical field of livestock breeding. The defects in the prior art are overcome. The method specifically comprises the following steps: monitoring internal environment parameters of a pig house and behavior postures of pigs in real time; analyzing the current perceptual comfort level of the pig by using a behavior-environment correlation model; and dynamically regulating and controlling the temperature control environment facility in the hog house according to the analysis result, wherein the monitoring mode of the internal environment parameters of the hog house is realized through an Internet of Things sensor network uniformly distributed at each key position in the hog house. The AI vision and the Internet of Things technology are fused, a closed-loop control system of pig behavior posture-environment parameter-growth performance is established, the pig behavior posture serves as a decision-making basis for regulating and controlling the environment in the pig house, the regulation and control response speed is effectively increased, parameters such as the temperature and humidity in the pig house are intelligently adjusted, and the control efficiency is improved. The pigs are in the best state 24 hours a day, and the production benefits are improved.

The invention discloses acinetobacter baumannii and application of the acinetobacter baumannii in construction of a mastitis animal model. The acinetobacter baumannii strain is acinetobacter baumannii AbST3475NMG202308, and the preservation number of the acinetobacter baumannii strain is CGMCC (China General Microbiological Culture Collection Center) No.34450 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Experiments prove that the acinetobacter baumannii has high pathogenicity and can be used for establishing a typical animal model caused by acinetobacter baumannii infection, the animal model can be specifically a mastitis animal model, and a certain technical support is provided for subsequent research and development of drugs or vaccines; the acinetobacter baumannii can also be used for screening antibacterial drugs and developing diagnostic reagents and vaccines. The method has an important application value.

The application belongs to the technical field of animal models for basic scientific research, and particularly relates to a method for establishing an acute lung injury animal model, which comprises the following steps: sending a podophyllotoxin or a podophyllotoxin reagent into a rat body through oral administration or intragastric perfusion to obtain the acute lung injury animal model. The method for establishing the acute lung injury animal model provided by the application uses the podophyllotoxin as an inducer for establishing a mouse acute lung injury model, and the mouse can orally take or intragastrically perfuse the podophyllotoxin to achieve the method, without needing a special medicine injection device, so that the method is simple in medicine taking, few in experimental steps, and does not need complex surgical operations.

The invention belongs to the field of agricultural cultivation application, and discloses a photocatalytic drawer type three-dimensional frog cultivation method, a plurality of frog cultivation drawers are arranged in a supporting frame in a three-dimensional mode, a photocatalytic reaction tank is arranged on the uppermost portion of the supporting frame, the photocatalytic reaction tank and a lowermost water purification drawer form a closed water circulation self-purification system, and a plant deep water purification structure is used in an auxiliary mode. And the wall of the supporting frame is surrounded by a photocatalytic net. The device has good ventilation and spraying water passing performance, frog breeding water closed circulation self-purification can be achieved, unit captivity or combined application can be achieved, screening, grading and fine management are convenient, land saving and efficiency increasing are achieved, frog breeding environment factors are optimized, morbidity is reduced, high-density healthy breeding is achieved, and in addition, the device can be used for breeding reptile aquatic products such as crabs and soft-shelled turtles.

Provided herein are compositions, methods, and uses involving antibodies that immunospecifically bind glycosylated forms of MUC16, a tethered mucin protein. Also provided herein are uses and methods for managing, treating, or preventing disorders, such as cancer.

The present invention provides, among other things, improved pharmaceutical compositions comprising codon-optimized mRNA encoding a peptide or polypeptide encapsulated in a lipid nanoparticle comprising one or more of the cationic lipids that are particularly effective for pulmonary delivery.

Compositions comprising microalgal extracellular vesicles (MEVs) formulated for intranasal delivery are provided, whereby upon intranasal administration, the MEVs are transported by a specific route, to specific regions in the brain via the olfactory nerve after intranasal administration, and to interconnected brain regions through the lateral olfactory tract (LOT). The MEVs are transported via neuronal axonal transport. The MEVs have the ability to cross synapses including: (i) synapses between olfactory sensory neurons (OSNs) and mitral / tufted neurons; (ii) synapses between mitral / tufted neurons and local neurons in multiple brain regions colonized by the lateral olfactory tract (LOT); and (iii) synapses between neurons in brain regions colonized by the LOT and neurons from the frontal cortex, hippocampus, thalamus, and hypothalamus. The compositions contain extracellular vesicles (MEVs) from microalgae loaded with bioactive cargo for the treatment, detection, diagnosis, or monitoring of diseases, disorders, or conditions of or involving the brain, particularly neuronal delivery of the cargo. The compositions and methods have multiple applications as therapeutic and diagnostic agents for the treatment, diagnosis, and monitoring of diseases, disorders, or conditions of or involving the brain. The compositions can be used in methods and uses for the treatment of cancers involving the brain, and can be used, for example, to deliver therapeutic agents for psychiatric diseases, disorders, conditions, and to deliver therapeutic agents for neurodegenerative diseases, disorders, and conditions.

The invention discloses an intelligent barley pest breeding system, and relates to the technical field of barley pest breeding, and the system comprises an environment regulation and control module, an automatic feeding module, a growth monitoring module, an intelligent processing module and an early warning prompt module. A full-life-cycle data acquisition system covering an egg stage, a larva stage, a pupal stage and an adult stage is constructed, AI image analysis of a visual monitoring unit can accurately identify insect body length and body surface abnormity, a physiological monitoring unit tracks body weight change and body temperature fluctuation in real time, and a growth prediction model is combined to predict the growth of insects. According to the method, the incubation time and key growth nodes of an ecdysis window are accurately pre-judged, the strategy optimization unit dynamically adjusts environment parameters based on the growth stage, cross-dimension correlation analysis of breeding data is achieved, data support is provided for formulating a standardized breeding process, and the industry is promoted to be changed into intelligent management of data driving from experience driving.

The invention relates to a pheretima bombycis farm, and belongs to the technical field of earthworm breeding. The problem that earthworm suffocation death is caused by cross contamination of pathogenic microorganisms and unsmooth drainage due to mixing of functional areas of a traditional farm is solved. The layout of the farm is specifically as follows: a raw material pretreatment and storage area provided with a fermentation tank is arranged in the upwind direction of a site entrance; a core breeding production area is arranged in the center of the field, the interior of the core breeding production area is divided into an earthworm breeding area, a breeding area and a fattening area according to breeding stages, and mechanical channels are arranged in the areas and are of a ridge type structure; arranging an annular drainage ditch along the boundary of the field, and communicating the furrow with the annular drainage ditch by gradient; a finished product treatment and storage area containing an airing field is arranged in the downwind direction of the field exit; an isolation belt is arranged on the periphery, a management and auxiliary area containing a tool room is arranged at an entrance, and a feces treatment area containing a sedimentation tank is arranged in the downwind direction; the hardened main road is connected with all the functional areas, and the branch is communicated with the breeding district. According to the scheme, pollution source isolation and efficient drainage are achieved, and the method is mainly used for improving the earthworm survival rate and breeding benefits.

The invention relates to the technical field of medicines, and discloses an application of a traditional Chinese medicine composition in a humanized flora transplantation model.The application comprises the following steps that an experimental body is pretreated through sterile feeding or broad-spectrum antibiotics, then coprophilous fungus suspension of a functional dyspepsia patient is transplanted, and the humanized flora transplantation model is constructed; acquiring physiological data of the humanized flora transplantation model, and performing intervention on the traditional Chinese medicine composition after the humanized flora transplantation model is completed; acquiring physiological data of the traditional Chinese medicine composition intervened humanized flora transplantation model, and analyzing an evaluation result of the traditional Chinese medicine composition intervened humanized flora transplantation model by combining the evaluation parameters of the humanized flora transplantation model. The bottleneck that the traditional animal model is greatly different from the human microflora and the curative effect and the mechanism are difficult to transform is overcome, so that the research result is closer to the clinical practice, and the molecular mechanism of the traditional Chinese medicine composition is reliably revealed.

A model that closely resembles human excessive myopia can be prepared by mounting a minus lens (2) and a protector (4) to a juvenile mouse, the minus lens having an angle and a width adjustable in response to growth of the mouse. Further, this model analysis shows that myopia induction causes endoplasmic reticulum stress in a sclera and the endoplasmic reticulum stress induces myopia. Furthermore, it is revealed that an endoplasmic reticulum stress suppressant, particularly, phenylbutyrate and tauroursodeoxycholic acid act as a myopia prevention / suppression agent.

The invention discloses a method for constructing an atrial fibrillation small animal model through succinic acid induction and application, and the method comprises the following steps: in an animal model construction period, enabling a small animal to ingest an aqueous solution containing succinic acid or all pharmaceutically acceptable salts of succinic acid every day, and finishing the modeling period to obtain the animal model. Compared with a traditional angiotensin AngII administration method, the atrial fibrillation small animal model has the advantages that the cost of a medicine for constructing the animal model is lower, the economic benefit is higher, and the atrial fibrillation induction effect is better; the model represents the most common atrial fibrillation type in clinic, and compared with an acute transient atrial fibrillation model, the research and application range is wider; compared with tail vein injection, intraperitoneal injection, subcutaneous pump burying and other modes, the non-creative model has the advantages that damage to small animals is smaller, and the requirement for the operation technology is low.

Disclosed are systems and methods for detecting extracellular ligands and / or inducing expression of an exogenous or endogenous gene. The disclosed systems and methods typically include and / or utilize (i) first and second exogenous extracellular sensors, and third and fourth exogenous extracellular sensors; and (ii) an expression vector comprising a target gene operably linked to a hybrid promoter sequence. The hybrid promoter sequence of the expression vector includes a minimal promoter for inducing transcription and the hybrid promoter sequence further includes interspaced transcription regulator binding sites upstream of the minimal promoter that bind two or more transcription regulators of the extracellular sensors that are released from the extracellular sensors when the extracellular sensor bind an extracellular ligand.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention relates to the technical field of disease animal models, in particular to a construction method, an evaluation method and application of a bipolar affective disorder liver stagnation and spleen deficiency syndrome combined animal model. According to the method, a liver depression and spleen deficiency syndrome modeling method is adopted for model construction, evaluation indexes are formulated by combining manic and depression behavior expressions of mice and innovatively combining typical expressions of liver depression and spleen deficiency, a liver depression and spleen deficiency syndrome animal model evaluation method is explored, and a thought is provided for achieving standardization and normalization of liver depression and spleen deficiency syndrome model evaluation. The modeling method is simple and economical, provides an experimental animal model for syndrome differentiation treatment of diseases, particularly provides a corresponding animal model for traditional Chinese medicine syndrome differentiation treatment and new drug pharmacological experiments, accelerates the progress of traditional Chinese medicine disease syndrome research and new drug development, provides technical service for drug evaluation, and has great application value.

The utility model relates to a drawer type parasitic wasp circulating feeding box which comprises a feeding layer box body with an opening in the upper end, an upper cover body capable of being pulled transversely is installed at the upper end of the feeding layer box body, a wasp releasing hole is formed in one side wall of the feeding layer box body, a blocking mechanism is installed on one side wall of the feeding layer box body, and a detachable filter screen is transversely arranged at the lower end of the feeding layer box body. And a garbage collection box body is detachably mounted at the lower end of the feeding layer box body and located on the lower side of the filter screen. The breeding box is compact in structure, not only facilitates breeding of parasitic wasps, but also facilitates replacement of breeding leaves and collection of garbage.

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.