89 results about "Antibody types" patented technology

Chimeric receptor genes suitable for endowing lymphocytes with antibody-type specificity include a first gene segment encoding a single-chain Fv domain of a specific antibody and a second gene segment encoding all or part of the transmembrane and cytoplasmic domains, and optionally the extracellular domain, of an immune cell-triggering molecule. The chimeric receptor gene, when transfected to immune cells, expresses the antibody-recognition site and the immune cell-triggering moiety into one continuous chain. The transformed lymphocytes are useful in therapeutic treatment methods.

Transgenic mice which constitutively express an antibody-type molecule encoded by the transgene and which has an IgE heavy chain constant region and is specific for a pre-defined antigen, provide an allergic reaction to that antigen without prior sensitization and are useful as allergy models.

The invention relates to an artificial antibody type titanium dioxide (TiO2)/ boron-doped diamond (BDD) membrane electrode and a preparation method thereof, the electrode is in p-n heterostructure, the electrode uses boron-doped diamond (BDD) as a substrate, uniform dense fusiform TiO2 particles are on the surface of the BDD, the particle size of the TiO2 particles is 50-100nm, a modification layer with a thickness of 5 to 10 mum is formed by the densely covered TiO2 particles; the preparation method of the artificial antibody type titanium dioxide (TiO2)/ boron-doped diamond (BDD) membrane electrode is as follows: using of the boron-doped diamond as the substrate, low temperature liquid phase deposition of TiO2 with a target pollutant molecular imprinting on the surface of the substrate, and removal of target pollutant molecules. Compared with the prior art, the artificial antibody type titanium dioxide (TiO2)/ boron-doped diamond (BDD) membrane electrode prepared by the method both has the p-n heterostructure and target pollutant molecular recognition ability, has excellent photoelectrocatalytic integrative performance and high selectivity degradation ability, has the advantages of simple preparation process, can be effectively used for removal of organic pollutants with low concentration, low tendency to biochemistry and high toxicity, and has extensive economic and social benefits.

The invention discloses a hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of the monoclonal antibodies thereof. Ciprofloxacin (CIP) coupled with bovine serum albumin is used as an antigen to immunize BALB/c mice and cell fusion, screening and cloning are carried out so as to obtain one hybridoma cell strain 1F1 capable of stable passage and secretion of monoclonal antibodies (MAb) to quinolones, wherein, the accession number of the hybridoma cell strain 1F1 is CGMCC No. 5608. The titres of ascitic fluids of the 1F1 monoclonal antibodies are up to 10<-7>, and the type and the subclass of the monoclonal antibodies are IgG1 and kappa chain. According to indirect competitive ELISA analysis, the 1F1 monoclonal antibodies perform specific reactions to quinolones like ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxacin, marbofloxacin, sarafloxacin and difloxacin. An ELISA method, a kit and test paper for detecting residual of quinolones in food are developed by using the 1F1 monoclonal antibodies.

The invention discloses a hybridoma cell strain secreting a monoclonal antibody against a barley yellow dwarf virus GAV strain, and an application of the monoclonal antibody thereof. A BALB/c mouse is immunized with barley yellow dwarf virus (BYDV) GAV strain virions which is used as antigens and is purified by a differential centrifugation method, and a hybridoma cell strain 27E1 which can be subcultured stably and secretes a monoclonal antibody against BYDV GAV is obtained through cell fusion, screening, and cloning, and the accession number is CGMCC No. 8781. The monoclonal antibody secreted by the hybridoma cell has an ascetic ELISA titer of more than 10<-6>, and the antibody type and subtype are IgG1, kappa chain. A specific reaction can be carried out between the monoclonal antibody secreted by the hybridoma cell strain 27E1 and the barley yellow dwarf virus GAV strain, but the monoclonal antibody does not react with rice stripe virus, rice ragged stunt virus, Chinese wheat mosaic virus, wheat yellow mosaic virus, barley yellow mosaic virus, barley yellow dwarf virus GPV strains and PAV strains. The hybridoma cell strain 27E1 and the secreted monoclonal antibody provide technical and material support for the diagnosis, detection and scientific prevention and control of wheat viral diseases.

The invention discloses an exosome nucleic acid detection technology based on magnetism-enriched electrochemical luminescence. The exosome nucleic acid detection technology comprises the steps of sample pretreatment and separation of an exosome standard substance, activation of streptavidin magnetic beads and a biotin antibody, binding of immunomagnetic magnetic beads and an exosome, preparation of exosome nucleic acid sample preparation and target nucleotide sequence detection through electrochemical luminescence. The exosome nucleic acid detection technology has the advantages that exosomesin biological samples such as urine, serum, saliva and a cell culture fluid can be separated and analyzed; antibodies are modified to the surfaces of the magnetic beads through specific binding of biotin and streptavidin, systems are stable, and nonspecific interference is avoided; the systems are controllable, different subtypes of exosomes are separated and purified by changing antibody types, and the technology can be used for nucleic acid expression profile studies and exosome somatotype studies; the final concentration is controllable, required concentration is obtained through re-dilution or re-releasing after magnetic enrichment, and follow-up detection is facilitated; the separation speed is high, the instrument requirement is low, and multiple samples can be treated simultaneously.

The invention provides a preparation method of a European American type pig propagation and syndrome virus antibody differential diagnosis Kit. The method constructs the pronucleus expression vector pGEX-6P-1-N aimed at American type PRRSV N protein regarding the gene deficiency of the conserved epitopes 25aa-30aa, 37aa-52aa and 50aa-66aa of European and American type strain in an ORF7 gene of a PRRSV VR-2332 strain. Regarding the amino acid sequence of the European type PRRSV N protein epitopes 2aa-12aa, 40aa-46aa and 67aa-128aa, the method establishes the pronucleus recombination expression vector PQE30-N of the European type PRRSV N protein. The recombination plasmid expresses successfully in the bacillus coli, and an indirect ELISA diagnosis Kit for PRRS antibody detection is prepared through using the expression depurated North American type N protein. The coincidence rates of the antibody Kit and an IDEXX Kit both reach 90 percent, and the depurated European type protein serves as enveloping antigen, thereby optimizing the European type PRRSV antibody detection indirection ELISA method, and laying a foundation for PRRS antibody typing detection.

Disclosed is a magnetic nanostructure for detecting and isolating circulating tumor cells including a conductive polymer to which an antibody and magnetic nanoparticles are bound, which enables circulating tumor cells from early cancer patients and various circulating tumor cell types to be effectively detected using a small amount of blood, circulating tumor cells to be monitored with the naked eye through colorimetric detection, and a very small amount of circulating tumor cells present in blood to be efficiently captured with a strong magnetic field generated by a large amount of the loaded magnetic nanoparticles; in detecting, isolating, and collecting the circulating tumor cells in a very small amount, a long nanowire structure and various antibody types are used, whereby contact with cancer cells may be increased and strong bonding may be formed; sensitivity is increased and various interactions with cancer cells are facilitated, thereby exhibiting increased detection and isolation effects.

The invention discloses a hybridoma cell strain secreting potato-virus-Y(PVY)-resistant monoclonal antibodies and monoclonal antibody application thereof. Pure PVY virion purified through a differential centrifugation method serves as antigens to immune BALB/c mice, cell fusion, screening and cloning are performed, one hybridoma cell strain 3B2 which is stable in passage and can secrete PVY-resistant monoclonal antibodies is obtained, and the preservation number of the hybridoma cell strain 3B2 is CGMCC No. 12001. The ascites indirect ELISA titer of the monoclonal antibodies secreted by the cell strain reaches 10<-7>, according to the antibody type and the subclass, the monoclonal antibodies are composed of IgG1 and kappa light chains, and the monoclonal antibodies and PVY coat protein of 30 kDa have a specific reaction. The 3B2 monoclonal antibodies are used for establishing ACP-ELISA, dot-ELISA and Tissue blot-ELISA detecting methods of PVY on crops, wherein the diseased leaf detecting sensitivity of the ACP-ELISA method and the diseased leaf detecting sensitivity of the dot-ELISA method reach 1:81920 and 1:10240 fold dilution (w/v, g/mL) respectively. Preparation of the PVY-resistant monoclonal antibodies and establishment of the detection methods of the PVY-resistant monoclonal antibodies provide matter and technologic support for detection and diagnosis, epidemiological analysis and scientific prevention and control of potato virus diseases.

The present invention discloses a fusion gene of compound antibody type with glyphosate and glufosinate, encoded protein and application thereof, The fusion gene composes of a glyphosate-tolerant encoded-protein gene and a glufosinate-tolerant encoded-protein gene. The glyphosate encoded gene is one of the following: CP4, aroA, G7, G10, GOX or GAT; the glufosinate encoded gene is bar or pat. The compound protein can give compound antibodies to the plants, glyphosate and glufosinate; it can be used to produce transgene plants with the antibody property of glyphosate and glufosinate. The provided compound protein with antibody properties of glyphosate and glufosinate can be used in herbicides for monocotyledonous plants and dicotyleons; it is mainly used in herbicides for insect resistant corn, rice, soybean, wheat and rape.

The invention discloses a hybridoma cell strain capable of secreting a tomato yellow leaf curl virus (TYLCV) monoclonal antibody and application of the monoclonal antibody. A coat protein gene of a tomato yellow leaf curl virus separator (TYLCV-SH2) is cloned; the coat protein of the virus is expressed by a prokaryotic expression system; BALB/c mice are immunized by using expression and purification protein as antigen; a hybridoma cell strain D10 capable of performing passage stably and secreting the TYLCV monoclonal antibody is obtained through cell fusion, screening and cloning; and the collection number is CGMCC No.5538. The D10 monoclonal antibody ascites indirect ELISA valence reaches more than 10<-6>; and the antibody type and subclass are IgG1 and kappa chains. A dot-ELISA detection method for detecting the TYLCV of the tomatoes is established by the D10 monoclonal antibody; and when the leaf suffering from the disease is diluted according to the ratio of 1:320 (w/v and g/mL), the virus can still be detected. By the dot-ELISA and Tissue-blot ELISA method, the TYLCV of tomato samples in the field can be detected accurately, specifically and sensitively. Due to establishment of a preparation method for the TYLCV monoclonal antibody and a detection method, technological and material support is provided for diagnosis, prediction and scientific prevention and control of the tomato virus disease.

The invention relates to a parathyroid hormone kit and a preparation method thereof, belongs to the technical field of immunologic diagnosis, and solves the technical problems that a parathyroid hormone method in the prior art is low in accuracy, short in shelf life, complicated in operation, unstable in test result and high in cost. The kit disclosed by the invention comprises a reagent R1, a reagent R2 and a reagent R3; the reagent R1 is a buffer solution I containing streptavidin magnetic particles; the reagent R2 is a parathyroid hormone monoclonal antibody solution labeled with a chemiluminescent marker diluted by the buffer solution I; and the reagent R3 is a coupled labeled parathyroid hormone monoclonal antibody solution diluted by the buffer solution I. The kit detects parathyroidhormone by adopting a chemiluminescence immunoassay, and has the advantages of high detection sensitivity, accurate quantitative detection, simple operation, no radioactive risk, short detection timeand capability of detecting antigen and antibody type target substances.

The invention discloses an antibody type biological magnetic microsphere as well as a preparation method and application thereof. On one hand, the invention provides the biological magnetic microsphere, at least one polymer with a linear main chain and branched chains is fixed onto the outer surface of a magnetic microsphere body, and tail ends of the branched chains of the polymer of the biological magnetic microsphere are connected with antibody type labels. The invention also provides a preparation method and application of the biological magnetic microsphere provided by the invention. The biological magnetic microsphere provided by the invention is convenient in operation and use and can be rapidly dispersed and rapidly settled in a solution, and large-scale experimental facilities such as a high-speed centrifugal machine are not required to be used; the biological magnetic microsphere is an antibody magnetic bead, and the antibody type labels can also be connected to the tail ends of the branched chains of the polymer through interaction of an affinity compound; and the use is wide, and the antibody type labels as a purification medium have flexible selectivity, and are universally and massively applied to separation and purification of a target object.

The invention discloses a hybridoma cell strain excreting a monoclonal antibody (MAb) resisting a rice blackstreaked dwarf virus (RBSDV) and the application of the MAb. A coat protein (CP) of the RBSDV is expressed into antigen immune BALB/c mouse by a prokaryotic expression method, and the hybridoma cell strain 5G1 which can stably passage and excrete the MAb resisting the RBSDV is obtained through cell fusion, selection and cloning, wherein the preserving number is CGMCC No. 5537. Ascitic indirect enzyme-linked immuno sorbent assay (ELISA) valence of the 5G1 MAb is over 10<-6>, and the antibody type and the subtype are IgG1 and kappa chain. The antibody excreted by the cell strain and the coat protein of the RBSDV have specific immunobinding reaction. By the dot-ELISA detection method for detecting rice planthopper and the RBSDV in rice and established by using the 5G1 MAb as a core, the virus can be still detected when a single-head small brown rice planthopper is diluted to 1,600 microlitres and sick leaves are diluted (w/v, g/mL) in the proportion of 1:160. The preparation of the MAb resisting the RBSDV and the establishment of the detection method thereof provide technical and material support for the diagnosis, forecast and scientific prevention and control of rice viruses.

The invention discloses a hybridoma cell strain secreting citrus yellow vein clearing viru(CYVCV)-resistant monoclonal antibodies and monoclonal antibody application thereof. Coat protein of prokaryotic-expression CYVCV serves as antigens to immune BALB/c mice, cell fusion, screening and cloning are performed, one hybridoma cell strain 18H5 which is stable in passage and can secrete CYVCV-resistant monoclonal antibodies is obtained, and the preservation number of the hybridoma cell strain 18H5 is CGMCC No. 12003. The ascites indirect ELISA titer of the monoclonal antibodies secreted by the cell strain reaches 10<-7>, according to the antibody type and the subclass, the monoclonal antibodies are composed of IgG1 and kappa light chains, and the monoclonal antibodies and CYVCV coat protein subunits of 32 kD have a specific reaction. The 18H5 monoclonal antibodies are used for establishing TAS-ELISA, dot-ELISA and Tissue blot-ELISA detecting methods of CYVCV in citrus trees, wherein the diseased leaf detecting sensitivity of the TAS-ELISA method and the diseased leaf detecting sensitivity of the dot-ELISA method reach 1:2560 and 1:20480 fold dilution (w/v, g/mL) respectively. Preparation of the CYVCV-resistant monoclonal antibodies and establishment of the detection methods of the CYVCV-resistant monoclonal antibodies provide matter and technologic support for detection and diagnosis, epidemiological analysis and scientific prevention and control of citrus virus diseases.

The invention discloses a monoclonal antibody hybridoma cell line for secreting southern rice black-streaked dwarf resistant viruses and monoclonal antibody application thereof. Polypeptide of 12 amino acids at the C end of a capsid protein of southern rice black-streaked dwarf viruses (SRBSDVs) coupled with bovine serum albumin is used as an antigen to immune a BALB/c mouse, one hybridoma cell line 3F1 capable of performing stable passage and secreting an SRBSDV monoclonal antibody is obtained through cell fusion, screening and cloning, and the preservation number of the hybridoma cell line 3F1 is China general microbiological culture collection center number (CGMCCNo.) 5535. Monoclonal antibody ascites indirect enzyme-linked immunosorbent assay (ELISA) valence of the 3F1 reaches above 10-6, an antibody type and a subclass are immunoglobulin G (IgG1) and a kappa chain, the monoclonal antibody of the hybridoma cell line has specific reaction with a capsid protein subunit of SRBSDV56kD, and a method for detecting rice planthoppers and dot-ELISA of SRBSDV in rice is built by using the monoclonal antibody of the 3F1. When a single-head white backed rice planthopper is diluted to 6400mul, the viruses can still be detected when diseased leaves are diluted according to 1:320 times (w/v, g/mL). The preparation of the SRBSDV resistant monoclonal antibody and the building of the detection method provide technology and material supports for diagnosis, prediction and scientific prevention and control of the rice viruses.

The invention discloses a hybridoma strain capable of secreting monoclonal antibodies for preventing tobacco mosaic virus (TMV) and monoclonal antibody applications thereof. The purified TMV particles are taken as the antigen to immunize a BALB/c mouse, through the steps of cell fusion, screening, and cloning, a hybridoma strain 3A7, which can stably pass the characteristics to the next generation and secret monoclonal antibodies for preventing TMV, is obtained, and the preservation number of the hybridoma strain is CGMCC No.9338. The ascites indirect ELISA titer of monoclonal antibody secreted by the hybridoma strain can reach 10-8, and the antibody type and subtype are IgG1, kappa chain. The monoclonal antibody secreted by the hybridoma strain can carry out specific reactions with TMV. A dot-ELISA virus detection method is established on the basis of the 3A7 monoclonal antibody and is used to detect TMV in plants such as tobaccos, and the like. The hybridoma strain 3A7, the monoclonal antibody secreted by the hybridoma 3A7, and the establishment of related serum detection method provide technological and material support for the detection, diagnosis, and scientific prevention and control on TMV.

The invention discloses a hybridoma cell strain capable of secreting a monoclonal antibody with resistance to maize chlorotic mottle virus and an application of the monoclonal antibody. The hybridoma cell strain capable of stably propagating and secreting a monoclonal antibody with resistance to MCMV (Maize Chlorotic Mottle Virus) can be prepared by taking virus particles of purified maize chlorotic mottle virus (MCMV) as antigen immune BALB/c mice, carrying out cell fusion, screening and cloning; the preservation number of the hybridoma cell strain is CGMCC No.9339; the monoclonal antibody secreted by the hybridoma cell strain has ascites indirect ELISA valence which reaches 10<-8>; the type and subclass of the antibody is IgG1; a chain is a kappa chain. The monoclonal antibody only has specific reactivity with MCMV; an immunological detection method is built by 24F6 monoclonal antibody and is used for detecting MCMV in virus-transferring amboceptor thrips, maize plates and seeds; the hybridoma cell strain 24F6 and the monoclonal antibody are acquired and a relevant serological detection method is built, so that the technical support is provided for the quarantine, detection, prediction and scientific prevention and control of the virus disease.

The invention discloses a hybrid tumor cell strain capable of secreting a sugarcane-mosaic-virus-resistant Yunnan strain monoclonal antibody and application of the monoclonal antibody of the hybrid tumor cell strain. Purified sugarcane mosaic virus (SCMV) particles serve as antigens to conduct immunity on BALB/c mice, cell fusion, screening and cloning are conducted, a hybrid tumor cell strain 22A6 which can conduct passage stably and secrete the SCMV Yunnan strain monoclonal antibody is obtained, and the preservation number is CGMCC No. 12007. The indirect ELISA titer of ascites of the monoclonal antibody secreted by the hybrid tumor cell strain reaches 10<-7>, and the antibody type and subclass are an IgG1 light chain and a kappa light chain. The antibody has a specific immunity response with SCMV shell protein of 36kDa; the 22a6 monoclonal antibody is utilized as the core to establish an ACP-ELISA method and a dot-ELISA method for detecting SCMV in corn, and the sensitivity of the two methods for detecting diseased leaves reaches 1: 163840-time attenuation (w/v, g/mL) and 1: 5120-time attenuation (w/v, g/mL). Obtaining of the hybrid tumor cell strain 22A6 and the monoclonal antibody thereof and establishment of a serological detecting method provide material and technological support for diagnosis and detection of the viral disease, breeding for disease resistance and scientific prevention and control.

The invention discloses application of a hybridoma cell line for secreting a monoclonal antibody resisting rice strip mosaic virus (RSMV) and the monoclonal antibody of the hybridoma cell line. The purified RSMV is an antigen immune BALB/c mouse; a hybridoma cell line 1D4 which can stably pass and secrete the monoclonal antibody resisting the RSMV is obtained by cell fusion, screening and colonizing, and the preservation number of the hybridoma cell line 1D4 is CGMCC (China General Microbiological Culture) No.14898. The ascites indirect ELISA (Enzyme Lined Immunosorbent Assay) titer of the monoclonal antibody secreted by the hybridoma cell reaches 10<-10>, and the type and subclass of the antibody are IgG1 and kappa chains. The monoclonal antibody has specific reaction with N protein of the RSMV. An ACP-ELISA method and a dot-ELISA method for detecting the RSMV are established by taking the 1D4 as a core; the sensitivities of the two methods for detecting disease leaves respectively reach the dilution of 1 to 2621440 and the dilution of 1 to 40960 (w/v, g/mL). The obtaining of the hybridoma cell line and the monoclonal antibody thereof and establishment of a serological testing method provide material and technical support for detection and diagnosis and scientific prevention and control of virus disease.

The invention relates to the field of biotechnology and discloses a nucleic acid detection method and a test strip. According to the method, an upstream primer with tag sequence and a downstream primer with a first counterpart are designed according to nucleic acid to be detected; nucleic acid to be detected undergoes PCR amplification; an amplification product undergoes denaturation and the denaturation product is combined with a colloidal gold-coated second counterpart; the combination product is then respectively combined with a detection line capable of capturing target detection objects; and result interpretation is carried out according to developing condition of the detection line. According to the invention, the target nucleic acid is captured through the tag sequence and anti-tag sequence, and the target nucleic acid can be specifically detected at room temperature. By the tag sequence and anti-tag sequence, the problem that the antigen-antibody types in the traditional antigen-antibody colloidal gold technology are few is overcome, and parallel detection of various target detection objects can be realized. The detection method of the invention is simple to operate, can be adopted to read results by naked eyes, is time-saving and labor-saving, and is more beneficial to practical application.

A method for synthesizing an antiserum for rapid-turnaround therapies includes collecting antibody-secreting cells from a test subject, wherein the test subject has been exposed to a target biological agent and has produced an antibody response; selecting a subset of the antibody-secreting cells, the subset of the antibody-secreting cells producing antibodies that neutralize the target biological agent; generating variable-region-coding DNA sequences from the antibodies that neutralize the target biological agent; tagging amplicons of the variable-region-coding DNA sequences with unique nucleic acid identifiers to associate the variable-region-coding DNA sequences derived from individual ones of the subset of the antibody-secreting cells; analyzing antibody-type distribution in a natural immune response; synthesizing antibodies from the variable-region-coding DNA sequences to form synthetic antibodies; and mixing the synthetic antibodies in a proportion equal to the antibody-type distribution in the natural immune response to form the antiserum.