Hybridoma cell strain secreting potato-virus-Y-resistant monoclonal antibodies and monoclonal antibody application thereof

Abstract

The invention discloses a hybridoma cell strain secreting potato-virus-Y(PVY)-resistant monoclonal antibodies and monoclonal antibody application thereof. Pure PVY virion purified through a differential centrifugation method serves as antigens to immune BALB/c mice, cell fusion, screening and cloning are performed, one hybridoma cell strain 3B2 which is stable in passage and can secrete PVY-resistant monoclonal antibodies is obtained, and the preservation number of the hybridoma cell strain 3B2 is CGMCC No. 12001. The ascites indirect ELISA titer of the monoclonal antibodies secreted by the cell strain reaches 10<-7>, according to the antibody type and the subclass, the monoclonal antibodies are composed of IgG1 and kappa light chains, and the monoclonal antibodies and PVY coat protein of 30 kDa have a specific reaction. The 3B2 monoclonal antibodies are used for establishing ACP-ELISA, dot-ELISA and Tissue blot-ELISA detecting methods of PVY on crops, wherein the diseased leaf detecting sensitivity of the ACP-ELISA method and the diseased leaf detecting sensitivity of the dot-ELISA method reach 1:81920 and 1:10240 fold dilution (w/v, g/mL) respectively. Preparation of the PVY-resistant monoclonal antibodies and establishment of the detection methods of the PVY-resistant monoclonal antibodies provide matter and technologic support for detection and diagnosis, epidemiological analysis and scientific prevention and control of potato virus diseases.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows researchers to create stable immune responses against plant diseases caused by tomatoes or pepperweeds that are infected through both classical and genetic techniques like ELISA (Enzymatic Isolation Immunospatial Fluorescence). These experiments demonstrate how well they have been able to identify certain types of bacteria called pvindyrosporium which causes food poisoning when ingested from crops grown on this type of crop. By measuring their ability to produce special proteins made up mostly of polypeptides containing eight amino acids, we found out about them being effective at identifying different microorganisms involved in causing symptoms associated with those illnesses.

Problems solved by technology

This patented problem addressed in this patents relates to controlling or reducing damage from pvilliviruses like Tobacina luteoviride, Borrelia bacteriophila, Boleta tularis, Toxiconductorum sppage, Plutonimorphora nematodes, Panama yellow fever, Listeriobergenesis, Xanthomonas axolobiae, Phytosporidium spinosynthotoxicum, Helminthes vibrissae, Nitraria solani, Sorghum syribe, Stellate lycopersites, Bactrospirachne cytopathodefense complex, Anonymous Spray Drift Research Group Limited describes various techniques utilizing pure polyporus viricles as an immunogenerative substance called AntiroVirna®. These research groups explored ways to create stable cloned copies of these viral genomes containing certain types of protective genetic materials. They then developed new technologies related to producing non-molluscated crop products based on their ability to transmit diseases between humans.

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