107results about How to "Sensitive detection" patented technology

The invention relates to the technical field of high-throughput sequencing, and discloses a kit and a library construction method and a target variation detection and analysis method thereof, the kit comprises a pre-library construction reagent and a target region liquid phase hybridization capture reagent; wherein the pre-library construction reagent comprises a repair reagent, a joint, a connection reagent, an amplification reagent and purified magnetic beads; the target area liquid phase hybridization capture reagent comprises a hybridization reagent, a cleaning reagent, an amplification reagent, a purification magnetic bead, a target gene probe and a CNV / LOH probe. The invention provides a rapid, accurate and low-cost construction method of a library containing a targeting gene probe and a CNV / LOH probe and application of the library, through one-time library construction and sequencing, the advantages of a high-throughput targeting sequencing technology and a CNV-Seq technology are integrated; multi-gene point mutation, insertion and deletion, gene copy number variation, large-fragment copy number variation and heterozygosity deletion can be detected at the same time.

The invention discloses an immunodetection method based on holographic imaging mode conversion and deep learning, and belongs to the field of immunoassay detection. The deep learning holographic mode conversion system constructed based on the non-paired data set reduces the influence of diffraction interference on target quantification to the greatest extent, so that holographic super-resolution imaging is realized, and challenges in paired data set generation are overcome; besides, a functional glass slide is designed, and rapid and sensitive holographic imaging detection is realized in combination with a click reaction signal amplification strategy; the functionalized glass slide is combined with the portable holographic imaging equipment, so that the detection efficiency of the system is greatly improved while accurate signal amplification is carried out, the application scene is expanded, and antibiotic residues can be rapidly and sensitively detected. The invention opens up a new research direction for high-sensitivity on-site convenient detection of trace target objects in portable equipment and complex samples.

The invention discloses a coarse cereal quality evaluation method and system based on a characteristic spectrum, and relates to the technical field of coarse cereal quality evaluation.The coarse cereal quality evaluation method comprises the following steps that light of a specific wave band is emitted to the surface of quinoa through a hyperspectral imager, and spectral data reflected by the surface of the quinoa are collected; the chenopodium quinoa willd surface spectrum change is captured through the spectrum extreme value attenuation index and the spectrum width index, the spectrum characteristic change coefficient is generated in combination with the machine learning model, and accurate judgment of mildewing of different degrees is achieved. Aiming at insufficient reflection caused by an oxide layer, the light emission duration is dynamically adjusted based on a Bayesian optimization algorithm, reliable spectral information is ensured to be obtained, and the adaptability and the detection precision are improved. In a complex environment, the system can stably and accurately identify mildew, delay is avoided, and high-quality data is provided for coarse cereal quality evaluation.

The invention discloses a rapid streptococcus agalactiae detection kit based on RPA (recombinase polymerase amplification) and CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats) and application of the rapid streptococcus agalactiae detection kit. The rapid streptococcus agalactiae detection kit comprises an amplification primer of a recombinase polymerase amplification technology, CRISPR RNA (ribonucleic acid), an ssDNA (single-stranded deoxyribonucleic acid) probe and Cas12a protein Wherein an upstream primer sequence of an amplification primer of the recombinase polymerase amplification technology is as shown in SEQ ID NO: 1; and the sequence of the downstream primer is as shown in SEQ ID NO: 2. According to the invention, rapid, sensitive, accurate and specific detection of streptococcus agalactiae is realized.

The invention discloses a self-powered sensor for detecting sugarcane smut pathogenic bacteria and a preparation method thereof, the self-powered biosensor comprises an anode, a cathode and an electrolyte, the anode is an Au / N-GDY / GOD / BSA biological anode, the cathode is an Au / N-GDY / DNA chain biosensor, and the electrolyte comprises glucose, [Ru (NH3) 6] 3 + and a PBS buffer solution. The sensor provided by the invention is used for portable detection of pathogenic bacteria of sugarcane smut, and quantitative analysis of a target analyte is realized through change of power output of the enzyme biological fuel cell. The target object can be simply, quickly, sensitively and efficiently detected.

The invention relates to a specific chemiluminescent probe for determining CYP3A in the technical field of medicines, which is structurally characterized by being shown in the following formula: # imgabs0 #. The probe structurally comprises a high-specificity reaction response group of CYP3A, a chemiluminescent group, a self-hyperchromic group and a self-leaving linking group connected with the high-specificity reaction response group, the chemiluminescent group and the self-hyperchromic group, the chemiluminescent probe does not need to be excited by an external light source, is free of phototoxicity, has extremely high signal-to-noise ratio and sensitivity, can be used for cell and living body imaging, has the advantages of being free of interference of biological matrixes, different in detection mode and the like compared with a traditional fluorescent probe, guarantees the accuracy of experiments, has the characteristics of detection convenience and high-throughput detection, and is suitable for large-scale popularization and application. The potential application value is realized in new drug research and development and individualized drug treatment.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) kit for detecting African swine fever viruses I and II and application of the multiplex fluorescent quantitative PCR kit. The multiplex fluorescent quantitative PCR detection reagent comprises a first primer probe group used for detecting a B646L gene, a second primer probe group used for detecting an MGF110 gene and a third primer probe group used for detecting an EP402R gene. The multiplex fluorescent quantitative PCR detection reagent for detecting the African swine fever viruses, provided by the invention, is high in sensitivity and good in specificity, and can be used for rapidly and sensitively detecting the African swine fever viruses I and II.

The invention discloses a method for rapid broad-spectrum detection of salmonella, and relates to the technical field of bacterial detection. Comprising the following steps: (1) preparing a labeled SERS probe; (2) preparing a Fe3O4 (at) Au (at) Ag magnetic composite material; (3) combining Fe3O4 (at) Au (at) AgNPs with Ag (at) 4MBA (at) AuNPs to construct an aptamer sensor; and (4) carrying out SERS detection on the salmonella. According to the novel surface enhanced Raman scattering (SERS) aptamer sensor constructed on the basis of noble metal nanoparticles and a magnetic composite material, according to the inverse proportion relation that the higher the salmonella concentration is, the weaker the Raman signal is generated according to the principle of a competition law, the ultrasensitive rapid field detection is carried out on different salmonella.

The invention discloses a method and a device for rapid in-situ monitoring of sodium precipitation at a negative electrode side of a soft-package sodium-ion battery. According to the method, the on-line nondestructive monitoring of the negative electrode sodium precipitation is realized by monitoring the pressure change of the sodium ion battery cell in the charging and discharging process. A first-order differential curve and a second-order differential curve of the pressure of the battery cell are obtained and analyzed by measuring and recording the pressure curve of the battery cell in the charging process on line, and when the change rate of the pressure of the battery cell exceeds a set threshold value and the second-order differential curve of the pressure is always larger than 0 under the high SOC, the second-order differential curve can serve as an external indication signal for judging sodium precipitation of the negative electrode. According to the technical scheme, the occurrence of the sodium separation reaction of the negative electrode can be efficiently and sensitively detected without any modification or damage to the target sodium ion battery cell. The method provides a simple and effective lossless online monitoring means which is easy to integrate and popularize for effectively defining the safe use condition of the sodium ion battery cell and monitoring and predicting the healthy operation condition of the sodium ion battery.

The invention relates to the technical field of urinary tract infection detection, and discloses a phage-based rapid and accurate urinary tract infection detection method, which comprises the following steps: S1, constructing a CRISPR / Cas9 system; s2, constructing a homologous recombinant plasmid; s3, constructing three plasmids BL21; s4, constructing, screening, verifying and reporting a phage T4:: Nluc; according to the detection method, a CRISPR / Cas9 system is combined with a homologous recombination technology, so that the efficiency of bacteriophage gene editing is remarkably improved, based on the report bacteriophage T4:: Nluc, rapid, sensitive, specific, accurate, simple and convenient detection on clinical urinary tract infection escherichia coli is realized, and the method can be used for detecting an escherichia coli urinary tract infection sample which is not pretreated, and has a wide application prospect. The kit shows excellent detection timeliness and specificity, is a powerful tool for identifying clinical urinary tract infection escherichia coli, and provides a new technical path for timely and accurate diagnosis and effective treatment of clinical urinary tract infection.

The invention provides a method, a device and equipment for acquiring cores with different saturations based on nuclear magnetic resonance, which can be applied to the technical field of carbon emission and geological storage. The method comprises the steps that nuclear magnetic resonance testing is conducted on an initial rock core, an initial peak area corresponding to a nuclear magnetic resonance spectrogram is obtained, and the initial rock core represents a rock core obtained after a to-be-detected rock core is subjected to pressure applying and water injection treatment; a target peak area is determined based on a preset saturation degree and the initial peak area, the preset saturation degree is determined based on a target saturation degree of a target rock, and the target rock represents a rock in the reservoir environment; the difference value between the initial peak area and the target peak area when gas is injected into the initial rock core is detected, and the initial peak area is larger than the target peak area; and determining a target rock core based on the difference value and a preset difference threshold value. According to the method, damage to the rock core can be reduced, nondestructive and sensitive detection is achieved, and the acquisition requirements of the rock cores with different saturations are met.

The invention relates to the technical field of data model analysis, in particular to an old people living behavior analysis method for intelligent old people care. Comprising the following steps: acquiring multi-modal data of living behaviors of old people to obtain synchronized multi-modal data; preprocessing the synchronized multi-modal data, and performing feature extraction and optimization on the preprocessed multi-modal data to obtain comprehensive feature data; acquiring historical living behavior data of old people to obtain historical comprehensive feature data; and based on the historical comprehensive feature data, constructing a hierarchical time sequence dynamic neural network model to obtain a trained and verified hierarchical time sequence dynamic neural network model, and processing the comprehensive feature data through the trained and verified hierarchical time sequence dynamic neural network model to obtain an old people living behavior analysis result. The technical problems that the multi-modal data feature extraction of the living behavior is not accurate enough and the sensitivity of an analysis model is low in the aged living behavior analysis are solved.

The invention provides a liquid drop surface action testing method based on an acoustic suspension technology and application, the liquid drop surface action testing method adopts the acoustic suspension technology to realize high-frequency deformation of liquid drops without external force interference, and the high-frequency surface expansion modulus of a surfactant is measured and calculated; according to the method, the liquid drop deformation hindering error caused by needle contact in a traditional oscillation dropping method is avoided, the defects that the high-surfactant concentration low-frequency expansion modulus value is small, and the foam stability correlation is poor are overcome, the foam performance of the chemical oil displacement formula can be rapidly and accurately evaluated, and research and development of the chemical oil displacement formula are guided.

The present invention provides a molecular beacon probe and a kit thereof for detecting isoniazid-resistant Mycobacterium tuberculosis. The molecular beacon probe is composed of a single-stranded nucleic acid molecule labeled with a fluorescent excitation group and a quenching group, wherein the fluorescent excitation group is selected from any three of FAM, VIC, TEXRED, CY5, JOE, NED, TET, HEX, and TAMARA; and the fluorescent quenching group is selected from BHQ1, BHQ2, and / or TAMARA. The detection kit provided by the present invention rationally distributes primers and probes to two reaction systems, effectively avoiding mutual interference during the PCR reaction process, and at the same time has high sensitivity and specificity to pathogens, reduces the cost of detecting isoniazid resistance of Mycobacterium tuberculosis, improves detection efficiency and accuracy, and is conducive to rapid and accurate clinical diagnosis.

This invention relates to the field of valve technology, specifically disclosing an intelligent gas flow regulating valve that integrates leaked gas collection and monitoring. The valve body includes a valve shell and a valve core. A valve stem is located at the upper end of the valve core, and a support cylinder is located at the top of the valve shell. The valve stem has a cavity inside, and a magnetic rack is movably mounted on the side wall of the valve stem. A motor is located on the outer wall of the support cylinder, and the output end of the motor is connected to a gear meshing with the magnetic rack. A magnetic plate with the same polarity as the magnetic rack is movably mounted inside the cavity. The cavity also has a telescopic chamber, and a pressure sensor is located on the side wall of the telescopic chamber. Increasing the volume of the telescopic chamber controls the magnetic plate to move away from the magnetic rack, causing the magnetic rack to disengage from the drive gear. Consequently, the valve core falls, blocking the gas path between the inlet and outlet. This invention can not only monitor gas leaks in pipelines but also collect leaked gas and use it to block the gas supply to the household gas pipeline, preventing leaked gas from escaping into the surrounding environment and causing fire safety accidents.

This invention provides a pretreatment method for detecting metribuzin in soil and broad beans using liquid chromatography-mass spectrometry (LC-MS / MS). The method includes: weighing 10.0 g of soil into a 50.0 ml centrifuge tube, adding 10.0 ml of acetonitrile, vortexing for 3.0 min, adding 3.0 g of NaCl, vortexing for 1.0 min, centrifuging at 5000 rpm for 5.0 min, taking 1.5 ml of the supernatant and adding it to a 2.5 ml centrifuge tube containing 25.0 mg PSA, 25.0 mg C18, and 100 mg anhydrous MgSO4, vortexing for 1.0 min, centrifuging at 8000 rpm for 3.0 min, aspirating the supernatant with a 5.0 ml syringe, filtering through a membrane, and transferring to a 2.5 ml brown sample bottle. This invention is applicable to the detection of metribuzin residues in broad beans, a specialty crop grown in high-altitude regions.

The present invention relates to the field of biotechnology detection, and in particular to a primer and a probe and a kit for detecting Salmonella pullorum. The present invention provides a primer and a probe for detecting Salmonella pullorum, wherein the primer includes a primer for detecting Salmonella pullorum ( Salmonella Pullorum , SP) citE2 SP-F and SP-R of a gene target sequence, wherein the probe is modified with a fluorescent reporter group and a fluorescent quencher group. citE2 The primer pair SP‑F and SP‑R that specifically bind to the gene target sequence, combined with the probe for atopic recognition, can accurately and sensitively detect Salmonella pullorum.

The invention belongs to the field of underwater information detection, and particularly relates to a bottom-supported underwater multi-physics-field information detection device, which comprises a mounting rack, an information detection module, a buoyancy module and an information integration module, and is characterized in that the mounting rack comprises an open top-layer three-dimensional frame, a middle-layer three-dimensional frame and a bottom-layer three-dimensional frame, and the top-layer three-dimensional frame is provided with a sound releaser; an anchor block base is movably arranged at the bottom of the bottom-layer three-dimensional frame and is connected with an execution end of the sound releaser, an information detection module of the sound releaser is located on the top-layer three-dimensional frame, and the top-layer three-dimensional frame is of a fully-open and unshielded structure and has higher perceptibility to the outside, so that underwater information can be monitored more easily in an omnibearing and more sensitive manner; however, most of existing similar devices are of semi-closed or closed structures, signal sources received by information detection modules are unidirectional, and if information needs to be monitored in all directions, the information detection modules need to be arranged in multiple directions.

The invention provides an MOF-on-MOF composite material, an aptamer probe as well as a preparation method and application of the aptamer probe. The MOF-on-MOF composite material comprises an HKUST-1 material, the surface of the HKUST-1 material is coated with a Cu-BDC nanosheet; the synthesized HKUST-1 (at) Cu-BDC / Apt aptamer probe can specifically recognize AFB1, the aptamer probe can directly capture the AFB1 and adsorb the AFB1 to active sites on the surface of a material to reduce the nano-enzyme activity of the AFB1, so that quantitative colorimetric detection of the AFB1 is realized, and the aptamer probe has low detection limit, wide detection range, good selectivity and anti-interference performance on the AFB1 and can be used for detecting the AFB1. The AFB1 in a food sample can be rapidly, sensitively and selectively detected. In addition, the composite material has an adsorption effect and high catalytic activity on fungaltoxin, and AFB1 can be efficiently removed through the adsorption effect and the catalytic action.

The invention discloses a method for detecting NR2F1 antigen and a reaction pool thereof, belongs to the field of biotechnology, solves the problems of high detection cost of existing detection methods, and comprises the following steps: step A: preparing a second NR2F1 antibody solution modified by glucose oxidase; step B: preparing an electrode paper sheet of a working electrode modified by a first NR2F1 antibody; step C: inserting the electrode paper sheet in step B into the reaction pool, dripping NR2F1 antigen extract into the reaction pool, dripping the second NR2F1 antibody solution modified by glucose oxidase in step A, reacting for 1 to 3 minutes, dripping glucose solution, reacting for 20 to 40 seconds, and detecting the current; step D: dripping NR2F1 antigen extracts of different concentrations and repeating step C, and making a standard curve according to the relationship between the antigen concentration and the current value; step E: taking a sample to be tested for detection, and obtaining the NR2F1 antigen concentration value in the sample to be tested. The invention has the advantages of portable detection and low cost.

The application relates to the technical field of molds, and particularly discloses a hot runner structure of a semi-solid magnesium alloy die-casting mold, which comprises a main runner structure and at least one sub-flow nozzle connected with the main runner structure, a sub-flow cavity is formed in the sub-flow nozzle, a valve needle is arranged in the sub-flow cavity, an outer wall of the valve needle is sleeved with a spiral sleeve, an outer wall of the spiral sleeve is provided with spiral guide protrusions, and intervals for fluid passing are formed between the spiral guide protrusions and an inner wall of the sub-flow cavity; and the spiral sleeve is connected with a conductive mechanism for axial displacement. The hot runner structure of the application considers both flow path optimization and real-time state monitoring, so that the stability of a semi-solid magnesium alloy die-casting process and forming quality are improved.

The invention relates to the technical field of medical biological detection, provides a new application of FCN2, particularly relates to an application of FCN2 as a target spot in screening or treating Alzheimer's disease, and further provides a kit for performing early screening or curative effect evaluation on Alzheimer's disease by utilizing quantitative detection aiming at FCN2 and a medicine taking the kit as the target spot. The kit and the detection method disclosed by the invention are simple, convenient, reliable, short in period, high in specificity and easy to clinically popularize. The liver-derived plasma protein Ficolin-2 (FCN2) can be used as an early screening target and a drug treatment target of the Alzheimer's disease (AD), is particularly used as a kit and other medical equipment products to be applied, and has important significance on early discovery, precise treatment, curative effect monitoring and the like of high-risk patients.

This invention discloses a rapid method for detecting CTCs, belonging to the field of tetracycline detection technology. The invention uses a fluorescence spectrometer to measure the fluorescence intensity of the test solution containing fluorescent probes PEI-CuNCs and a surfactant. Compared with the fluorescence intensity of PEI-CuNCs, if a new blue fluorescence emission band appears at 425 nm and the blue-green fluorescence at 500 nm decreases, then the test solution contains CTCs. The detection method of this invention has advantages such as simple operation, speed, high sensitivity, good selectivity, low detection limit, and low cost. It does not require large-scale instruments or professional operators, and shows good spiked recovery rate in actual water sample testing, with reliable detection results and excellent practicality.

The present invention discloses a zwitterionic chemiluminescent reactive oxygen species probe that is cleared by the kidneys, and a preparation method and application thereof, and relates to the technical field of preparation of ROS-responsive fluorescent nanoprobes. The preparation method comprises the following steps: S1, using NaBH 3 CN is used as a reducing agent, and MSA-AuNPs protected by the thiol ligand MSA are prepared by chemical reduction; S2, ABEI is chemically coupled to the surface of MSA-AuNPs prepared in step S1 by covalent bonds to prepare nanoprobe ABEI-MSA-AuNPs. The present invention discloses a zwitterionic chemiluminescent reactive oxygen species probe that is cleared by the kidneys, and a preparation method and application thereof. The chemiluminescent molecule ABEI is chemically coupled to the surface of MSA-AuNPs with near-infrared fluorescence properties by covalent bonds to prepare nanoprobe ABEI-MSA-AuNPs. The zwitterionic effect induced by charge pairing effectively inhibits the protein adsorption of the nanoprobe in the body, increases the clearance rate of the nanoprobe by the kidneys, and can achieve rapid and sensitive detection of ROS levels in disease sites in the body by dual-channel ratio urine analysis.

The present invention relates to a capacitive induction detection and identification circuit, comprising a display or execution module, a processing unit, a capacitive induction detection processing conversion unit and an induction detection unit, wherein the display or execution module, the processing unit, the capacitive induction detection processing conversion unit and the induction detection unit are electrically connected in sequence. The beneficial effects of the present invention are as follows: the change in circuit charge caused by approaching an object is converted into a change in detection end capacitance and is detected, thereby realizing the detection of the approach or distance of the detected object, and the circuit can realize non-contact object detection, has a long service life, and is sensitive to detection.

This invention discloses a bivalent anti-Vibrio parahaemolyticus nanobody, its preparation method, a self-sandwich ELISA kit, and a detection method. The aim is to provide a bivalent anti-Vibrio parahaemolyticus nanobody, kit, and detection method with high accuracy, specificity, and sensitivity for detecting Vibrio parahaemolyticus. The amino acid sequence of the bivalent anti-Vibrio parahaemolyticus nanobody is shown in SEQ ID NO.7. This invention belongs to the field of biodetection technology.

The application discloses a method for determining multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, and relates to the technical field of drug and poison analysis and detection, and comprises the following steps: preparing a mixed standard working solution, wherein the mixed standard working solution contains paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride; and determining the multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, wherein the contents of paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride are determined simultaneously. The method is simple in operation, sensitive in detection and reliable in result, and is suitable for poisoning diagnosis and clinical toxicology research.

The invention discloses a primer probe group for detecting and distinguishing infectious pancreatic necrosis viruses 1 and 5 and application of the primer probe group. The invention provides a primer probe group for detecting and distinguishing infectious pancreatic necrosis virus type 1 and infectious pancreatic necrosis virus type 5. The primer probe group consists of a primer pair (SEQ ID No.1 and SEQ ID No.2) and a probe (SEQ ID No.3) for detecting the infectious pancreatic necrosis virus, a primer pair (SEQ ID No.4 and SEQ ID No.5) for identifying the infectious pancreatic necrosis virus type 1 and a primer pair (SEQ ID No.6 and SEQ ID No.7) for identifying the infectious pancreatic necrosis virus type 5. The primer probe group and the method provided by the invention can specifically, quickly and sensitively detect the IPNV and carry out typing of type 1 or type 5, and can be used for detecting the IPNV in samples of Atlantic salmon, rainbow trout and the like.

The present invention relates to a LAMP primer set for detecting BCR-ABL fusion gene, including a LAMP primer set for detecting BCR-ABL1 gene, a LAMP primer set for detecting BCR-ABL2 gene, and a LAMP primer set for detecting BCR-ABL3 gene, with a total of 17 LAMP primers, and the sequence is shown in SEQ ID NO: 1-17. Detection method: Using the sample DNA to be tested as a template, the above-mentioned LAMP primer set for detecting BCR-ABL1 gene, the LAMP primer set for detecting BCR-ABL2 gene, and the LAMP primer set for detecting BCR-ABL3 gene are used for loop-mediated isothermal amplification, and then the amplified product is detected, and judged according to the test result. The detection method of the present invention can quickly, sensitively and specifically detect three types of BCR-ABL fusion genes, and is simple to operate, does not require complex instruments, and has low detection cost.

The invention relates to the technical field of salmonella detection, and provides a photonic crystal fluorescence sensor based on recombinase polymerase amplification and a CRISPR / Cas12a system, and the photonic crystal fluorescence sensor comprises a biosensing platform and a photonic crystal array; the reaction components of the biosensing platform comprise an RPA reagent, an upstream primer, a downstream primer, Cas12a protein, crRNA, an ssDNA probe and a buffer solution; the photonic crystal array is mainly constructed by a PDMS substrate, polystyrene microspheres, ethanol and a microwell plate analyzer; the detection sensitivity of the salmonella is obviously improved.