2159results about How to "Sensitive detection" patented technology

A system and method for monitoring photodynamic therapy of a target tissue, where the target tissue contains a photosensitizing substance, include a first light source configured to deliver light to the target tissue, the first light source having a wavelength capable of exciting the photosensitizing substance. An ultrasonic transducer receives photoacoustic signals generated due to optical absorption of light energy by the target tissue, and a control unit in communication with the ultrasonic transducer reconstructs photoacoustic tomographic images from the received photoacoustic signals to provide an indication of optical energy deposition due to the photosensitizing substance in the target tissue.

A system and method for monitoring laser therapy of a target tissue include a therapeutic control unit having a first light source configured to deliver light to the target tissue for therapy, an ultrasonic transducer for receiving photoacoustic signals generated due to optical absorption of light energy by the target tissue, and a monitoring control unit in communication with the ultrasonic transducer for reconstructing photoacoustic tomographic images from the received photoacoustic signals to provide an optical energy deposition map of the target tissue. A second light source utilized for imaging may also be provided.

The present invention provides methods and kits for detecting susceptibility to, or the incidence of, cervical cancer in a sample comprising determining the methylation status of the ESR1 gene, optionally as part of a panel of genes. Also provided are methods and kits which involve determining the expression levels of genes including the ESR1 gene in order to diagnose cervical cancer.

The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

Methods for detecting cardiac injury by detecting one or more cardiac markers are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for a cardiac marker, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

A system and method for highly selective intrusion detection using a sparse array of ultra wideband (UWB) radars. Two or more UWB radars are arranged in a sparse array around an area to be protected. Each UWB radar transmits ultra wideband pulses that illuminate the area to be protected. Signal return data is processed to determine, among other things, whether an alarm condition has been triggered. High resolution radar images are formed that give an accurate picture of the area to be protected. This image is used to detect motion in a highly selective manner and to track moving objects within the protected area. Motion can be distinguished based on criteria appropriate to the environment in which the intrusion detection system operates.

An air filled trench is formed underneath the waveguide to reduce propagation loss, which in turn allowing the waveguide to be in the close proximity of on-chip devices, such as a photodetector. The air filled trench is formed from the back side of the substrate; hence it would not disturb the integration and the formation of components on the front side of the substrate. In another embodiment, for silicon-on-insulator (SOI) based device, with an air filled trench and a metal electrode, a back gate is formed. In yet another embodiment, air filled trench also reduces the substrate loss of RF passive components and passive antenna operating in Giga Hertz range. Air filled trenches can be used for both photonic and electronic circuits in a planar lightwave circuit. Finally, another embodiment is for the trench to effectively guide gases and fluids to pass through the detection area.

Methods and apparatus for analyzing bioprocess fluids are provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Porous members can be positioned so as to partially or fully span channels in microfluidic systems. The porous members can be assembled and/or disassembled in situ. The porous members can be made such that pores are separated by connections including but a single molecule at one location, allowing for a high level of open area in a very small pore size member. The porous member can be made up of colloid particles interconnected with molecular species. These can be used to detect analytes qualitatively and/or quantitatively, or to selectively bind and/or release agents on command for a variety of purposes including first blocking, then opening a channel, concentrating analyte over time followed by release of analyte and detection downstream, etc. Porous members can define valves in multiple-channel systems and, with controlled binding and release of agents at the porous members, these valves can be opened and closed and fluid flow controlled in a multi-channel system. Fluidic systems of the invention can include multiple sensing locations at which different analytes are determined. Systems of the invention provide flexibility for overall microchemical analysis, sequentially, of a variety of agents.

The invention provides to an external-framework type bidirectional force feedback data glove and relates to a force feedback data glove which is used for detecting human figure moving status and is provided with a bidirectional active drive to feed back force borne by an auxiliary hand. The external-framework type bidirectional force feedback data glove aims to resolve the problems that an existing force feedback data glove is separated in measurement mechanism and feedback mechanism, complex in system, tumid in structure, difficult to maintain, unachievable in bidirectional drive, expensive in price and the like. According to the external-framework type bidirectional force feedback data glove, a joint detection mechanism is connected with a force feedback mechanism into a whole, a unique connecting mode and an ingenious joint measurement point layout are used, five fingers of a user are enabled to keep the maximum flexibility when the force feedback data glove is used, the moving status of each joints of the fingers can be detected accurately, the movement of each finger joint of the controlled auxiliary hand is enabled to be coordinated with the movement of a corresponding finger joint of an operator, the force bearing conditions of the auxiliary hand in a specific working environment can be fed back to the operator, and the immediacy sense of a virtual reality or a remote operation is enhanced.

The invention discloses a method for detecting a piconet island state according to the self-characteristic of a piconet. The method comprises the following steps of: (1) detecting electrical characteristic value when the piconet runs; (2) judging whether the voltage at a load end is higher than the definite value or not, if yes, entering step (3), and if not, considering that the piconet is not in the island state; (3) judging whether the frequency changing rate is higher than the upper limit of the definite value or not, if yes, sending out a trip signal and then finishing, and if not, entering step (4); (4) detecting and judging whether the frequency changing rate is higher than the lower limit of the definite value or not, if yes, judging whether the phase difference is more than the phase difference setting valve or not, if yes, finishing the detection and sending out the trip signal, and if not entering step (5); and (5) after delaying for a period of time, judging whether the phase difference is more than the phase difference setting valve or not, if yes, sending out the trip signal, and if not, considering that the piconet is in the island state. Compared with the existing detection method, the method has the characteristics of rapid and reliable detection, and guarantees comparatively high anti-interference capability at the same time.

The invention relates to system and method for detecting the transformer winding state by using constant-current sweep frequency power source excitation. A measurement and control analysis module controls sweep frequency power source and applies output constant-current sweep frequency excitation signals by an exciting transformer at a high-voltage side of the transformer to be detected; a vibration sensor measures vibration response signals of a transformer winding to be detected at different excitation frequencies; a vibration signal collector collects and preprocesses the signals measured by the vibration sensor; and the measurement and control analysis nodule carries out spectrum analysis to the received and processed vibration response signal data, displays and records a resonant frequency curve of the transformer winding on a frequency domain, compares with the resonant frequency curve previously measured and recorded as well as the vibration frequency curve of a transformer three-phase coil, and judges the state of the transformer winding to obtain the measuring result. The invention can effectively and sensitively detect the states of looseness or deformation of the transformer winding for prompt repair or replacement so as to avoid a fault of abrupt short circuit of the transformer caused by damage of the winding structure.

Methods and systems for evaluating and/or monitoring chromatography column performance are provided. Embodiments apply multivariate analysis (MVA) methods to process data as well as transition analysis data to provide a comprehensive evaluation of chromatography column performance. In embodiments, transition analysis data generated over extended periods of time can be analyzed together with process data to evaluate column performance. Further, embodiments enable a compact and robust tool for combining and presenting performance evaluation results, which allows for time-efficient performance examination. According to embodiments, MVA methods applied on transition analysis and process data provide (1) near real-time ability to comprehensively monitor column packing quality; (2) sensitive detection of column integrity breaches; (3) sensitive detection of subtle changes in column packing; (4) sensitive detection of different types of changes in column packing; (5) sensitive detection of fronting/tailing; and (6) sensitive detection of changes in process performance.

The invention provides a cTn I (cardiac troponin I) hypersensitive detection kit and a preparation method thereof. The kit comprises at least one strain of a first anti-cTn I antibody marked with a tracing marker and at least one strain of a second anti-cTn I antibody coated with magnetic micro-spheres, wherein a binding site between the first anti-cTn I antibody and cTn I is different from a binding site between the second anti-cTn I antibody and cTn I; and the kit can preferably further comprise a diluent, and by adopting the diluent, the non-specific binding in a detection process can be significantly reduced, and the detection accuracy and sensitivity can be further improved. The invention also provides a method for detecting cTn I by using the kit, and the method provided by the invention has very high sensitivity, can be used for sensitively and accurately detecting the cTn I content in a sample, and can provide more timely and reliable information for early diagnosis and treatment of AMI.

The invention discloses a one-step nucleic acid test method based on CRISPR/Cas (clustered regularly interspaced short palindromicrepeats/CRISPR-associated protein) and isothermal amplification and akit. The method is a quick field test method used for testing nucleic acid and displaying visual test results through fluorescent light or lateral side immunochromatographic test paper. The test accuracy is effectively ensured through specificity of crRNA (RISPR-derived ribonucleic acid); at a room temperature, whether a test sample contains test nucleic acid can be determined through the test paper or the fluorescent light within 1-2h; and compared with the traditional test method, the precision, simplicity and convenience in operation of the test method are improved to some extent. The kit with purified freeze-drying LbaCas12a, crRNA and RPA (recombinase polymerase amplification) can be formed; a nucleic acid segment of a specific sequence can be conveniently and quickly tested on site;a complicated temperature control instrument such as a PCR (polymerase chain reaction) instrument and other electrophoresis and centrifugation equipment are not required; and nucleic acid of the specific sequence can be tested quickly and sensitively only by a thermostatic device and fluorescence detection equipment and even visual observation.

The invention provides preparation and application of a quantitative and rapid detection biosensor of microcystic toxin-LR, and belongs to the biotechnology field. The detection sensor is an impedance type electrochemical sensor, and consists of a CHI760C type electrochemical work station and a three-electrode system in which an immunity electrode is taken as a working electrode, a saturated calomel electrode is taken as a reference electrode and a platinum wire electrode is taken as a counter electrode; L-cysteine, colloidal gold and microcystic toxin antibody compound are respectively enveloped on a surface of a bare mitallic electrode to be taken as the working electrode; a change of electric potential difference between the reference electrode and the working electrode takes place when the microcystic toxin antibody is combined with the antigen (MC-LR) in sample solution, and is subject to a quantitative relation with the concentration of the antigen in the sample, then the detection of the microcystic toxin is carried out; the biosensor for rapidly, specifically and sensitively detecting microcystic toxin antigens is successfully established; the detection linear range is 0.05-300ng/mL, and the detection limit is 1.82*10<-2>ng/mL. The biosensor can perform real-time monitoring on natural lake water, and can also perform real-time monitoring on preparation process of drinking water in water supply works.

An unmet need of orthopaedic practitioners is a clinically-relevant measurement device and technique to objectively quantify joint (e.g., knee) stability, including rotational stability. An apparatus and method is disclosed for performing a mechanical joint movement test, such as a knee pivot shift test, through the application of standardized dynamic loads (e.g., using a constant-tension spring or other mechanism). When used for knee evaluation, this test reliably induces the characteristic “pivot” tibial subluxation/reduction event in an ACL-deficient knee, allowing for more sensitive detection of deficiencies in rotational knee stability, quantification of the relative importance of knee stabilizers, and comparison of the performance of contemporary ACL reconstruction techniques. The test allows for better understanding joint biomechanics and for defining unique kinematic and kinetic signatures for common joint injuries, such as knee injuries, that will be valuable for diagnostic purposes.

The invention relates to a manufacturing method of a graphene-porphyrin modified electrode and application of the electrode, aiming to solve the problems of complex operation, long time and relatively high price in the conventional method for detecting the content of ascorbic acid in a living body. The method comprises the following steps: I, preparing pre-oxidized graphene; II, preparing a graphene oxide solid; III, preparing acyl-chlorinated graphene; IV, preparing tetraphenylporphyrin; V, preparing 5-(4-nitro)-10,15,20-triphenyl porphyrin; VI, preparing 5-(4-amino)phenyl-10,15-20-triphenyl porphyrin; VII, preparing a pure manganese porphyrin coordination compound; VIII, preparing a functional composite material of graphene oxide and porphyrin; IX, modifying a glassy carbon electrode. The modified electrode has excellent interference resistance, stability and reproducibility, and can be taken as a biosensor for detecting ascorbic acid in practical samples.

The invention relates to a method for detecting bisphenol A and bisphenol AF in food, belonging to technical field of analytical chemistry determination method. With ionic liquid (1-butyl-3-methylimidazole hexafluorophosphate) as an extractant and TritonX-100 or acetone as an dispersant, ultrasonic oscillation is carried out to form emulsion; after centrifugal separation, extraction drops at the lower layer are directly subjected to HPLC (high performance liquid chromatography) quantitative analysis. The method disclosed by the invention has the advantages of simpleness in detection steps, easiness in operation, low detection limit and high enrichment multiple, greatly reduced detection cost. The method disclosed by the invention is a rapid, efficient and environmentally friendly pre-treatment technology and has a wider application prospect in the field of food analysis.

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I/D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

A field effect transistor-based rapid human tumor diagnosis apparatus comprises a field effect transistor biochip, a microfluidic channel sample packaging system and a set of electronic detection system. The semiconductor manufacturing technology is utilized to construct a source electrode, a drain electrode and a semiconductor channel between the source electrode and the drain electrode on a silicon substrate, silane reagent is covalently modified on the channel to serve as connecting molecules, which are connected with the specific antibody protein of a certain tumor marker, a microfluidic channel which is constructed with polydimethylsiloxane is then tightly attached and packaged on the silicon substrate, and finally, the source electrode and the drain electrode are connected with the signal detection system. By assaying the tumor marker in human blood, the tumor diagnosis apparatus can rapidly, accurately and early diagnose whether a patient suffers from a certain type of cancer, and is an integrated, high-flux and portable tumor diagnosis apparatus.

The invention provides a Taqman-MGB fluorescent quantitative PCR kit and a method for detecting 12 common viruses and bacteria of pigs at the same time. The kit comprises PCR reaction liquids A/B/C, wherein the PCR liquids comprise primer pairs and Taqman probes for porcine parvovirus (PPV), type-II streptococcus suis (SS-II), a porcine pseudorabies virus (PRV), type-II porcine circovirus (PCV-2), a hog cholera virus (CSFV), a pig foot and mouth disease virus (FMDV), a porcine reproductive and respiratory syndrome virus (PRRSV), a high pathogenicity porcine reproductive and respiratory syndrome virus strain (Hp-PRRSV), a transmissible gastroenteritis virus (TGEV), an epidemic diarrhea virus (PEDV), rotavirus (PRTV) and a swine influenza virus (SIV) respectively. 12 pathogens of pigs can be detected rapidly and effectively at the same time, the detection method is high in accuracy, specificity and sensitivity and is good in stability, and rapid diagnosis and effective detection on pathogens to be detected can be achieved.

The invention discloses an independent photoelectric smoke detection alarm which comprises an alarm base, an alarm upper cover and a circuit board. The alarm upper cover covers the alarm base to form a photoelectric darkroom, and only smoke and less light are allowed to enter the photoelectric darkroom. A space is surrounded by a lower recessed cavity in the alarm base and an upper recessed cavity in the alarm upper cover to form the photoelectric darkroom. The circuit board is arranged in the lower recessed cavity. A signal processing module, an infrared transceiver module and a wireless communication module are integrated on the circuit board. The infrared transceiver module comprises an infrared light emitting diode and an infrared receiving diode. A voice alarm module and a display module are built in the alarm upper cover. A test button is arranged on the alarm upper cover. A power supply module is arranged in the alarm base. The independent photoelectric smoke detection alarm provided by the invention has the advantages of strong anti-interference ability, sensitive detection, simple structure, reasonable design, accurate detection and long service life. A 3-volt lithium manganese battery with the service life of 10 years is built in the independent photoelectric smoke detection alarm.