25results about How to "Sensitive detection" patented technology

This invention relates to the field of valve technology, specifically disclosing an intelligent gas flow regulating valve that integrates leaked gas collection and monitoring. The valve body includes a valve shell and a valve core. A valve stem is located at the upper end of the valve core, and a support cylinder is located at the top of the valve shell. The valve stem has a cavity inside, and a magnetic rack is movably mounted on the side wall of the valve stem. A motor is located on the outer wall of the support cylinder, and the output end of the motor is connected to a gear meshing with the magnetic rack. A magnetic plate with the same polarity as the magnetic rack is movably mounted inside the cavity. The cavity also has a telescopic chamber, and a pressure sensor is located on the side wall of the telescopic chamber. Increasing the volume of the telescopic chamber controls the magnetic plate to move away from the magnetic rack, causing the magnetic rack to disengage from the drive gear. Consequently, the valve core falls, blocking the gas path between the inlet and outlet. This invention can not only monitor gas leaks in pipelines but also collect leaked gas and use it to block the gas supply to the household gas pipeline, preventing leaked gas from escaping into the surrounding environment and causing fire safety accidents.

This invention provides a pretreatment method for detecting metribuzin in soil and broad beans using liquid chromatography-mass spectrometry (LC-MS / MS). The method includes: weighing 10.0 g of soil into a 50.0 ml centrifuge tube, adding 10.0 ml of acetonitrile, vortexing for 3.0 min, adding 3.0 g of NaCl, vortexing for 1.0 min, centrifuging at 5000 rpm for 5.0 min, taking 1.5 ml of the supernatant and adding it to a 2.5 ml centrifuge tube containing 25.0 mg PSA, 25.0 mg C18, and 100 mg anhydrous MgSO4, vortexing for 1.0 min, centrifuging at 8000 rpm for 3.0 min, aspirating the supernatant with a 5.0 ml syringe, filtering through a membrane, and transferring to a 2.5 ml brown sample bottle. This invention is applicable to the detection of metribuzin residues in broad beans, a specialty crop grown in high-altitude regions.

The invention provides an MOF-on-MOF composite material, an aptamer probe as well as a preparation method and application of the aptamer probe. The MOF-on-MOF composite material comprises an HKUST-1 material, the surface of the HKUST-1 material is coated with a Cu-BDC nanosheet; the synthesized HKUST-1 (at) Cu-BDC / Apt aptamer probe can specifically recognize AFB1, the aptamer probe can directly capture the AFB1 and adsorb the AFB1 to active sites on the surface of a material to reduce the nano-enzyme activity of the AFB1, so that quantitative colorimetric detection of the AFB1 is realized, and the aptamer probe has low detection limit, wide detection range, good selectivity and anti-interference performance on the AFB1 and can be used for detecting the AFB1. The AFB1 in a food sample can be rapidly, sensitively and selectively detected. In addition, the composite material has an adsorption effect and high catalytic activity on fungaltoxin, and AFB1 can be efficiently removed through the adsorption effect and the catalytic action.

The application relates to the technical field of molds, and particularly discloses a hot runner structure of a semi-solid magnesium alloy die-casting mold, which comprises a main runner structure and at least one sub-flow nozzle connected with the main runner structure, a sub-flow cavity is formed in the sub-flow nozzle, a valve needle is arranged in the sub-flow cavity, an outer wall of the valve needle is sleeved with a spiral sleeve, an outer wall of the spiral sleeve is provided with spiral guide protrusions, and intervals for fluid passing are formed between the spiral guide protrusions and an inner wall of the sub-flow cavity; and the spiral sleeve is connected with a conductive mechanism for axial displacement. The hot runner structure of the application considers both flow path optimization and real-time state monitoring, so that the stability of a semi-solid magnesium alloy die-casting process and forming quality are improved.

This invention discloses a rapid method for detecting CTCs, belonging to the field of tetracycline detection technology. The invention uses a fluorescence spectrometer to measure the fluorescence intensity of the test solution containing fluorescent probes PEI-CuNCs and a surfactant. Compared with the fluorescence intensity of PEI-CuNCs, if a new blue fluorescence emission band appears at 425 nm and the blue-green fluorescence at 500 nm decreases, then the test solution contains CTCs. The detection method of this invention has advantages such as simple operation, speed, high sensitivity, good selectivity, low detection limit, and low cost. It does not require large-scale instruments or professional operators, and shows good spiked recovery rate in actual water sample testing, with reliable detection results and excellent practicality.

This invention discloses a bivalent anti-Vibrio parahaemolyticus nanobody, its preparation method, a self-sandwich ELISA kit, and a detection method. The aim is to provide a bivalent anti-Vibrio parahaemolyticus nanobody, kit, and detection method with high accuracy, specificity, and sensitivity for detecting Vibrio parahaemolyticus. The amino acid sequence of the bivalent anti-Vibrio parahaemolyticus nanobody is shown in SEQ ID NO.7. This invention belongs to the field of biodetection technology.

The application discloses a method for determining multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, and relates to the technical field of drug and poison analysis and detection, and comprises the following steps: preparing a mixed standard working solution, wherein the mixed standard working solution contains paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride; and determining the multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, wherein the contents of paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride are determined simultaneously. The method is simple in operation, sensitive in detection and reliable in result, and is suitable for poisoning diagnosis and clinical toxicology research.

The application discloses an antibody against ochratoxin A and application thereof. The application uses an artificial antigen obtained by coupling ochratoxin A and a carrier protein as an immunogen to prepare a monoclonal antibody against ochratoxin A. The monoclonal antibody can sensitively and specifically detect ochratoxin A, and the minimum detection limit (LOD) is 0.18 ng / mL. In addition, the half inhibition (IC 50 ) of the icELISA for ochratoxin A established by using the monoclonal antibody against ochratoxin A is 1.42 ng / mL, and the linear range is 0.34-5.40 ng / mL. That is, the application provides an antibody capable of detecting ochratoxin A and a method for detecting ochratoxin A, and has the advantages of simplicity, rapidness, strong specificity, wide linear range and high sensitivity.

The power voltage generator includes a voltage sensor and a power circuit breaker. The voltage sensor is configured to sense a first voltage during a first charge-sharing period of the gate clock signal and a second voltage during a second charge-sharing period of the gate clock signal. The power circuit breaker is configured to disconnect power based on the first and second voltages.

This invention discloses a method for preparing and applying a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure. Based on an epitaxial layer-by-layer growth strategy, this invention constructs a multi-layered core-shell structure to obtain a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure, ZIF-8@coumarin@ZIF-8@rhodamine B, exhibiting an "inner layer reference - outer layer response" relationship. The nanoprobe prepared by this invention not only suppresses signal crosstalk caused by fluorescence resonance energy transfer but also achieves spatial selectivity of the inner filtration effect. Based on the characteristic absorption of malachite green in the 600-620 nm range, it can selectively quench the fluorescence of the outer rhodamine layer while maintaining a stable signal from the inner coumarin layer, thus achieving ratiometric fluorescence detection. This detection method has advantages such as strong anti-interference ability, high signal stability, and high sensitivity, and can be used for the rapid detection of malachite green in aquatic products.

This invention discloses a method for preparing a hierarchical frustum-shaped porous synergistic light-trapping SERS substrate and its application, belonging to the field of surface-enhanced Raman spectroscopy and microfluidics. The preparation steps include: S1, self-assembly of monolayer PS microspheres onto a PI film; S2, formation of a frustum structure on the PI surface via ICP etching; S3, removal of residual PS microsphere template by toluene dissolution and formation of a micron-scale pit array using PDMS molding; S4, self-assembly of monolayer PS nanospheres within the micron-scale pits and covering the nanosphere array with SU-8 photoresist; S5, cross-linking of SU-8 with UV exposure and removal of the nanospheres to form nanopores; S6, electron beam evaporation of a gold film; S7, substrate characterization; S8, substrate functionalization; S9, fabrication of a microfluidic chip and sealing with the SERS substrate; S10, heavy metal ion trace detection. The hierarchical frustum-shaped porous synergistic light-trapping SERS substrate proposed in this invention exhibits excellent structural uniformity and chemical stability, good oxidation resistance, and high-density hotspots. Its micro-nano synergistic structure can effectively improve light energy utilization efficiency and form abundant surface plasmon resonance regions, thereby enhancing Raman scattering signals, which has important application value for trace detection of analytes.

The utility model discloses a kind of monitoring devices of broken resin, including being arranged on the fine treatment mixed bed fine treatment mixed bed import sampling point and fine treatment mixed bed export sampling point, fine treatment mixed bed import sampling point and fine treatment mixed bed export sampling point are connected with cooling device entrance, cooling device outlet is divided into two routes, one road is connected with fine treatment mixed bed import on-line TOCi analyzer and DCS host computer display system, another road is connected with DCS host computer display system through fine treatment mixed bed export on-line TOCi analyzer.The utility model can monitor mixed bed inlet and outlet water TOCi value and its change trend in real time.Once detection value is abnormally increased (indicating resin broken leakage), system can send alarm in time, it is convenient for fast response and processing, avoid water vapor system pollution.

This invention relates to the field of power equipment condition monitoring and intelligent diagnosis technology, specifically to a transformer fault location method and system based on a palladium thin-film hydrogen sensor array. The system deploys a palladium thin-film resistive hydrogen sensor array at multiple key locations in the transformer tank to collect real-time dynamic changes in hydrogen concentration in the oil. A virtual transformer fault dataset generated from a digital twin model of the transformer's operating state is used for training using artificial intelligence algorithms. The trained model can intelligently analyze and match features from sensor signals collected during actual operation, thereby achieving rapid identification and spatial location of internal transformer faults. This invention, by integrating the high sensitivity of palladium thin-film resistive sensors, the simulation capabilities of a digital twin system, and the data-driven characteristics of artificial intelligence algorithms, achieves early warning, fuzzy location, and intelligent diagnosis of transformer faults, offering advantages such as fast response, high accuracy, and strong scalability.

The application belongs to the technical field of biological detection, and aims at solving the single color change mode and poor sensitivity of the current ELISA kit, and provides CMO nano-oxidase, a CMO-OVA-MC-LR probe, a kit, a preparation method and application thereof.The CMO nano-oxidase is a raspberry-shaped Co-doped Mn3O4 oxidase, the nano-oxidase is a two-heterojunction CMO of Co3O4 and Mn3O4 synthesized by one-pot hydrothermal method, and the particle size is 110-140 nm.The detection limit of the enzyme-linked immunosorbent kit constructed by the application for microcystin LR (MC-LR) is 0.505 ng / mL, and the quantitative range is 0.195-100 ng / mL.Benefiting from the strong catalytic activity and good biocompatibility of the nano-enzyme, the label and signal response mode open up a new way for the sensitivity improvement of the immunosensor determination, and are conducive to further expanding the application of the immunosensor.

The application relates to the technical field of concentration monitoring, and discloses a concentration monitoring method and system based on multi-modal behavior analysis, which comprises the following steps: acquiring a facial video stream, a body posture video stream and a screen content image of a user; processing the facial video stream and the body posture video stream by adopting a space-time feature recursive aggregation network, extracting multi-scale behavior features, calculating the staying time of a visual line landing point in each semantic region of the screen content image based on the facial video stream, calculating a visual inertia coefficient for each semantic region in combination with the content complexity of the semantic region, constructing a weighted visual line transfer matrix according to the visual inertia coefficient, extracting a main eigenvalue as a visual focus stability measure, calculating and monitoring the instantaneous value of head shaking impact, adjusting the model parameters of the space-time feature recursive aggregation network when the instantaneous value exceeds a preset threshold, and calculating a real-time concentration score of the user. The application improves the comprehensiveness and reliability of concentration monitoring by fusing multi-dimensional and deep features.

The utility model provides a fire extinguishing device based on a cable joint, which belongs to the technical field of cable accessories and comprises an outer sheath, a mounting seat, a butt joint block, a flange seat, a mounting tube, a sealing plug, a limiting plate, a fire extinguishing agent storage bottle, a container valve, a nozzle, a temperature sensing glass ball, a thermosensitive joint, a stamping fire detection tube and a pressure gauge, according to the utility model, the mounting pipe is welded on the side surface of the outer sheath, the fire extinguishing agent storage bottle is mounted in the mounting pipe through the limiting plate, and the stamping fire detection pipe on the fire extinguishing agent storage bottle is adhered to the inner wall of the outer sheath through the adhesive tape; when the stamping fire detection tube meets fire, the stamping fire detection tube is softened and exploded at the highest heated temperature position, the container valve is started through pressure reduction in the stamping fire detection tube, dry powder in the fire extinguishing agent storage bottle is released to the nozzle through the release tube to extinguish fire, the dry powder is filled in the outer sheath and rapidly extinguishes the fire when the fire breaks out from the source, the fire is effectively restrained from spreading outwards, and the fire extinguishing effect is improved. And loss caused by cable fire is reduced.

This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The MNP marker site includes at least one of MNP-1 to MNP-19. The primer set includes at least one primer pair from the first to the 19th primer pair. The forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the 19th primer pair, and the reverse primer of the 19th primer pair are shown sequentially as SEQ ID NO: 1 to SEQ ID NO: 38 in the sequence listing. This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The primer set targets 19 MNP marker sites, and each pathogen can be detected by 1 to 3 marker sites. It can identify multiple pathogens in wastewater, providing comprehensive, efficient, and accurate detection, and is suitable for identifying multiple pathogens in wastewater.

The invention relates to the technical field of flexible sensor preparation, and discloses a preparation method and application of a ZIF-8-based flexible pressure sensor with a wide linear range. According to the flexible sensor provided by the invention, a sensing solution with high sensitivity and a wide linear range is realized through an innovative design of growing a metal organic framework (MOF) compound. The preparation method comprises the following steps: immersing the cotton fabric in a carbon nanotube dispersion liquid to obtain a carbon nanotube conductive fabric, and immersing the obtained conductive fabric in a ZIF-8 growth solution to obtain the flexible pressure sensor based on ZIF-8. The sensor has the advantages of high sensitivity, wide linear range, quick response and good stability and repeatability, can be applied to the fields of gesture recognition and intelligent control, intelligent health monitoring, environment monitoring and the like, and has a wide application prospect.

This invention belongs to the field of pharmaceutical technology, specifically relating to a FRET-based cell sensor for detecting botulinum toxin types A and E and its applications. The invention unexpectedly discovered the use of EGFP / tDimer2 as the donor and acceptor fluorophores, and SNAP-25 as the linker, to fuse and express EGFP-SANP-25-tDimer2. A cell sensor was constructed by transiently transfecting Neuro-2a cells with a plasmid expressing EGFP-SANP-25-tDimer2. This cell sensor can be applied to assess the bioactivity of materials containing BoNT / A and BoNT / E in the environment and during pharmaceutical manufacturing. It can also meet the in vitro screening requirements for botulinum toxin inhibitors and can be used for the diagnosis of BoNT / A and BoNT / E botulinum toxin poisoning.

This utility model discloses a steel wire diameter detection platform, relating to the field of steel wire detection technology. It aims to solve the problem that most existing steel wire diameter detection devices use a single-direction detection method, making it difficult to comprehensively and accurately detect the steel wire diameter and prone to detection blind spots. The measuring housing has a fixed plate inside, on which a detection component is mounted. The detection component has multiple sets of annular arrays. A limiting component is located on the other side of the fixed plate, connected to the detection component via a connecting component. By setting up four sets of annular array detection components on the steel wire diameter detection platform, the steel wire diameter can be detected from two directions, enabling comprehensive and blind-spot-free acquisition of steel wire diameter data, effectively avoiding detection blind spots. Compared with traditional single-direction detection methods, this significantly improves the accuracy and reliability of the detection results.

The invention discloses a nopinone carbazole ratiometric fluorescent probe for detecting benzoyl peroxide as well as a preparation method and application of the nopinone carbazole ratiometric fluorescent probe. The fluorescent probe is BTHC-CV-BB (beta, beta-dihydroxybenzene)- The method comprises the following steps: by taking THC as a raw material, carrying out substitution reaction on THC and 1-bromobutane to obtain a compound BTHC; the preparation method comprises the following steps: carrying out formylation reaction on a compound BTHC to obtain BTHC-C; the compound BTHC-C and 4-pyridine acetonitrile are subjected to a condensation reaction, and BTHC-PC is obtained; the compound BTHC-PC and 4-bromomethyl phenylboronic acid pinacol ester are subjected to a quaternization reaction, and the fluorescent probe BTHC-CV-BB is obtained. A solution of the probe BTHC-CV-BB reacts with benzoyl peroxide, and under the sunlight, the color of the solution becomes colorless from pink; under the irradiation of ultraviolet light with the wavelength of 365 nm, the fluorescence color of the solution is changed from red to blue-green. The compound BTHC-CV-BB can sensitively detect the content of benzoyl peroxide in a solution, the detection range is 0-90 [mu] M, the lowest detection limit is 32 nM, the corresponding time is 5 min, and the compound BTHC-CV-BB has the characteristics of high sensitivity, high response speed, good selectivity, wide detection range and the like, and has a good application prospect as a fluorescent probe for detecting benzoyl peroxide.

This invention relates to the field of fluorescence sensing materials technology, specifically to a biporous cationic covalent organic framework material, its preparation method, a ratiometric fluorescent probe, and its applications. The unit cell parameters of the biporous cationic covalent organic framework material are: a = 72.3305 Å, b = 72.3305 Å, c = 3.7877 Å, α = β = 90.000°, γ = 120.000°; its structure is shown in Formula I. This invention utilizes the strong electrostatic interaction between the nanoscale pore wall ion sites of the biporous cationic covalent organic framework material and the charged groups of fluoroquinolone antibiotics, combined with a spatial matching effect, to achieve highly selective recognition of FQs.

The application provides a bispecific nanobody against SARS-CoV-2 and related nucleic acids, proteins, cell products and applications thereof. The bispecific nanobody is constructed by concatenating two specific nanobodies targeting different epitopes of the novel coronavirus in a specific manner, and exhibits excellent neutralization activity against each type of variant strain of the novel coronavirus, has the significant advantages of broad-spectrum and high-efficiency anti-novel coronavirus infection, and provides an effective treatment strategy for preventing or treating infection of the novel coronavirus and its variant strains in the clinic. In addition, the bispecific nanobody can also achieve sensitive and reliable detection of the novel coronavirus.

The invention relates to the field of chemical analysis, in particular to a digestion method and a detection method for ICP-MS (Inductively Coupled Plasma Mass Spectrometry) detection of cationic guar gum. The invention relates to a digestion method for cationic guar gum ICP-MS detection. The digestion method comprises the following steps: S1, accurately weighing a sample; s2, pre-digestion: enabling the sample to be in full contact with concentrated nitric acid; s3, adding hydrogen peroxide; s4, carrying out digestion in a high-pressure tank; and S5, post-processing: fixing the volume for later use. The ICP-MS determination method using the digestion method comprises the following steps: A1, preparing a standard curve and an internal standard solution; a2, ICP-MS instrument condition determination: using helium as collision gas; and A4, calculating a result. According to the method, a nitric acid-hydrogen peroxide and high-pressure tank assisted digestion technology is adopted to thoroughly decompose organic macromolecules, and then the digested solution is detected by using an ICP-MS method, so that the characteristic elements in the cationic guar gum can be accurately and sensitively measured.

This invention relates to a multimodal imaging capsule endoscopy system, comprising: a capsule endoscope, an external wireless power supply module, and an image receiving and processing module. The capsule endoscope includes a white light and narrow band imaging module for acquiring white light and narrow band images, an autofluorescence imaging module for acquiring autofluorescence images, a wireless transmission module for transmitting the white light, narrow band, and autofluorescence images to the external image receiving and processing module, a light source control module for controlling the on / off state of different light sources within the capsule endoscope, a wireless power supply receiving module for providing power to the capsule endoscope, a position control module for controlling the position and orientation of the capsule endoscope in the gastrointestinal tract, and a capsule shell encapsulation. The external wireless module provides power to the capsule endoscope via the wireless power supply receiving module. The image receiving and processing module receives and analyzes images acquired by the capsule endoscope via the wireless transmission module. Compared with existing technologies, this invention achieves sensitive, accurate, and non-destructive detection of early-stage atypical hyperplasia or superficial gastrointestinal cancer, significantly reducing missed detections and false positives.