33 results about "Immunochromatographic test" patented technology

This invention provides an assembled high-throughput immunochromatographic test strip array sample application device, comprising a 96-well plate fixing bracket (1) and a test strip support plate (2); immunochromatographic test strips (3) are arranged and fixed by slots (4) and limiting blocks (5) on the test strip support plate (2), with their sample application areas (6) protruding from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device. This device can be used for high-throughput sample application in immunochromatographic detection.

The utility model is designed for the rapid (up to 20 minutes) detection of at least 10 types of analytes in environmental samples (water, air, soil, etc.) and clinical specimens. The immunochromatographic test strip for multiplex analysis includes a polymer substrate with an applied immunochromatographic membrane and a capture pad. The test and control zones of the test strip are designed as two rows of transverse microstrips occupying no more than half the width of the test strip. The test strip includes a marker for coordinating the cutting of test strips. The technical result consists in reducing the time for recording the luminescent signal from test strips in an automated system. 3 c.p. phyl., 3 fig., 2 tables.

The utility model discloses a three -dimensional solid bipolar ECL lateral flow immunity test paper strip and sensor, three -dimensional solid bipolar ECL lateral flow immunity test paper strip including three -dimensional solid bipolar electrode piece, lateral flow immunity test paper strip and connecting pad, three -dimensional solid bipolar electrode piece is below, lateral flow immunity test paper strip and connecting pad are above, three -dimensional solid bipolar electrode piece adopts the stratified design, including electrode piece layer one and electrode piece layer two, electrode piece layer two is below, and electrode piece layer one is above, electrode piece layer two includes male drive electrode, male drive electrode includes male drive electrode head, male drive electrode tail and its connecting wire, the utility model discloses first design a kind of three -dimensional solid bipolar electrode piece, the electrode piece includes electrode piece layer one and electrode piece layer two, and it is combined by having adhesive back glue, this breaks the shortcoming of traditional bipolar ECL electrode piece, and it is more suitable for the demand of on -the -spot rapid detection.

This invention belongs to the field of molecular biology detection technology, specifically relating to a Nipah virus (NiV) nucleic acid detection kit combining RT-LAMP and immunochromatography, and its application. This kit combines the high sensitivity and specificity of loop-mediated isothermal amplification (LAMP) technology with the rapid and visual interpretation advantages of immunochromatographic test strips, enabling on-site, rapid, and accurate detection of NiV. The core components of this invention include: a set of specific primers designed for conserved regions of the NiV gene, an optimized RT-LAMP reaction system, and specially treated immunochromatographic test strips for detecting LAMP amplification products. This kit eliminates the need for reverse transcription of RNA virus nucleic acids and expensive thermal cycling equipment. The entire detection process is completed within 20–30 minutes, with a sensitivity of up to 300 copies / μL. This solution combines the high efficiency of RT-LAMP technology with an intuitive, equipment-free detection method, achieving truly rapid and accurate on-site diagnosis of NiV.

The application discloses a signal reading method, system, device and medium for a multi-target array test strip, and relates to the technical field of fluorescent immunochromatographic detection. The method collects an RGB fluorescent image of the multi-target array test strip; a binary image is obtained through grayscale processing and adaptive threshold segmentation; an effective signal contour is extracted; a spatial position calibration and automatic sorting of discrete fluorescent signals are performed through an intelligent sorting algorithm combining DBSCAN clustering and principal component analysis; non-uniform background interference is removed through a robust local regression method to obtain a purified signal; and finally, real RG ratio signal values of each target point are extracted. The application solves the problems of difficulty in multi-target positioning and incomplete background removal of the existing array test strip signal reading method through an intelligent algorithm, realizes accurate positioning, automatic sorting and efficient purification of multi-target fluorescent signals, and significantly improves detection accuracy. The application is suitable for quantitative analysis of multi-target immunochromatographic test strips.

This invention provides a fully automated immunoassay analyzer, comprising: a base, a reaction disk for carrying multiple immunochromatographic test strips installed inside the base, a sample processing area for processing samples installed on the base, and a moving arm assembly movably installed on the base for driving a pipette arm to perform liquid pipetting, mixing, and spotting operations in the sample processing area. Compared with the prior art, this invention has the following advantages: by setting up the moving arm assembly, in conjunction with the pipette arm and negative pressure nozzle, its automated operation can simultaneously perform multiple sample single detection, single sample multiple detection, or multiple sample multiple detection, with high throughput, making up for the insufficient throughput of existing manual experiments. Automated sample pretreatment and automatic recycling of waste after experiments avoid manual errors and are convenient and pollution-free.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

This invention discloses an immunochromatographic assay method for the simultaneous detection of α-AMA and PCD, comprising the following steps: S1. Adding the test sample and an immunoprobe for detecting α-AMA and PCD to an external reaction vessel, allowing the test sample to pre-react with the immunoprobe, wherein the immunoprobe for detecting α-AMA and PCD is a COF@CDs@mAbs probe; S2. Adding the reaction solution obtained in step S1 to the sample pad of the immunochromatographic test strip, and performing chromatography under capillary action; S3. After chromatography is completed, detecting the signals of the first detection line, the second detection line, and the control line, and performing qualitative or quantitative analysis of α-AMA and PCD in the test sample based on the signal changes of the first and second detection lines. This invention sets the first and second detection lines on the same nitrocellulose membrane, enabling the simultaneous analysis of α-AMA and PCD in a single detection process, which is beneficial for improving detection efficiency and reducing sample consumption.

The application relates to the technical field of immunochromatographic medical detection, in particular to a brain natriuretic peptide immunochromatographic test paper based on Yb 3+ core-shell shell upconversion nanocrystals, which comprises a PVC bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially connected and pasted on the PVC bottom plate; the nitrocellulose membrane is provided with a detection line T line and a quality control line C line which are separated from each other; the combination pad is loaded with a fluorescent detection probe and a quality control probe. Through the construction of a core-shell shell structure and the directional control of an energy gradient, near-infrared luminescence is significantly enhanced, and high-sensitivity quantitative detection is realized. Therefore, the problems of complex operation, low sensitivity, easy interference of sample background and difficulty in realizing accurate quantification of traditional brain natriuretic peptide detection methods are solved.

This invention discloses a highly specific and sensitive immunochromatographic test strip, comprising a first conjugation pad, a second conjugation pad, a nitrocellulose membrane coated with C and T lines, an absorption pad, and a base plate. The first conjugation pad is immobilized with antibody A conjugated to oxidized dextran, representing the antigen to be detected. The second conjugation pad is immobilized with ConA-conjugated colloidal gold and anti-DNP antibody conjugated to colloidal gold. The T line is coated with antibody B, representing the antigen to be detected. The C line is coated with DNP. The invention also discloses the preparation method of the test strip, the detection kit, and the detection method. This invention introduces the dextran and ConA system. ConA is a tetrameric globulin, with each subunit containing one sugar-binding site, and each ConA having four sugar-binding sites, thereby enhancing the detection signal, amplifying the signal, and improving detection sensitivity. It is suitable for the detection of various product types and has broad application prospects.

This invention belongs to the field of biotechnology detection, and particularly relates to an immunochromatographic test strip for monitoring the purification efficiency of acridine ester-labeled antibodies, comprising test strip A and test strip B; test strip A has a first detection line T1A and a second detection line T2A on its nitrocellulose membrane, as well as a control line CA; the first detection line T1A is coated with an anti-acrididine ester antibody, and the second detection line T2A is coated with an antigen that specifically binds to the antibody to be tested; test strip B has a first detection line T1B and a second detection line T2B on its nitrocellulose membrane, as well as a control line CB; the first detection line T1B is coated with an antigen that specifically binds to the antibody to be tested, and the second detection line T2B is coated with an anti-acrididine ester antibody. The immunochromatographic test strip provided by this invention for monitoring the purification efficiency of acridine ester-labeled antibodies is a simple, intuitive, rapid, and low-cost tool for monitoring purification efficiency.

The application discloses a three-dimensional bipolar ECL lateral flow immunochromatographic test strip and application thereof in immunodetection, and relates to the technical field of immunodetection. The three-dimensional bipolar ECL lateral flow immunochromatographic test strip comprises a three-dimensional bipolar electrode sheet, a lateral flow immunochromatographic test strip and a connecting pad; the three-dimensional bipolar electrode sheet is arranged below, the lateral flow immunochromatographic test strip and the connecting pad are arranged above; the three-dimensional bipolar electrode sheet is designed in a layered mode and comprises an electrode sheet layer one and an electrode sheet layer two; the electrode sheet layer two is arranged below, and the electrode sheet layer one is arranged above; the electrode sheet layer two comprises a positive driving electrode; the positive driving electrode comprises a positive driving electrode head end, a positive driving electrode tail end and connecting wires thereof. The three-dimensional bipolar electrode sheet is designed for the first time, the electrode sheet layer one and the electrode sheet layer two are combined through back glue with adhesion, the shortcomings of a traditional bipolar ECL electrode sheet are broken, and the three-dimensional bipolar ECL lateral flow immunochromatographic test strip is more suitable for the demand of on-site rapid detection.

This application relates to an ethyl maltol hapten, a complete antigen, and their applications. The ethyl maltol hapten prepared by this invention fully retains the negatively charged hydroxyl group on the pyran ring of ethyl maltol, and introduces a linear arm with an active group from the oxygen atom of the pyran ring, completely exposing the structural features of ethyl maltol and improving the immunogenicity of the antigen. The further prepared ethyl maltol complete antigen and monoclonal antibody exhibit high sensitivity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 4.5 μg / L; ethyl maltol complete antigen and monoclonal antibody are used in colloidal gold immunochromatography, which can quickly and conveniently detect ethyl maltol. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 1 µg / L for ethyl maltol.

This invention relates to the field of multispectral image fusion technology, specifically to an immunochromatographic quantitative algorithm and system based on multispectral image fusion. The algorithm acquires the detection area of ​​an immunochromatographic test strip and a visible light image Im(x,y) of the same detection area. Cross-modal registration is performed between the acquired multispectral signal Sp(λ) and the acquired visible light image Im(x,y). Through image contour recognition and spectral acquisition area calibration, the C and T regions of the test strip are accurately located and matched to obtain the registered target detection areas T and C. Baseline correction and feature extraction are performed on the multispectral signal Sp(λ) of the registered T and C regions to calculate the spectral feature vector Spf. The advantages of this invention are: by fusing multispectral signals and image signals, it compensates for the information limitations of a single modality, and combined with a robust temperature compensation and batch compensation mechanism, it achieves precise quantitative detection at the μg / mL or ng / mL level.

This invention belongs to the field of drug detection technology, and relates to a platinum-iridium bimetallic nanozyme, its preparation method, and its application in immunochromatographic detection. The method includes: first, preparing a bimetallic nanozyme with a platinum core and an iridium shell; then, conjugating it with an antibody to create a colorimetric enhanced immunolabel. Based on this, a competitive immunochromatographic test strip is constructed, with the corresponding drug antigen immobilized on the T line and a quality control capture agent immobilized on the C line. During detection, the sample and label are mixed and added dropwise. If the sample does not contain the target drug, the labeled antibody binds to the antigen on the T line, resulting in color development; if the target drug is present, it competes with the T line for binding to the labeled antibody, leading to a weakening of the T line signal. A colorimetric solution is added, utilizing the catalytic activity of the nanozyme to amplify the signal, significantly improving sensitivity. This invention achieves detection limits of 0.41 ng / mL for vancomycin and 0.12 ng / mL for chlorpheniramine maleate, exhibiting advantages such as speed, high sensitivity, and ease of operation, making it suitable for therapeutic drug monitoring and trace drug residue detection.

The present invention provides a method for amplifying a signal in an immunoassay test for high sensitivity detection of an analyte and an immunoassay kit for carrying out the method.

This invention discloses a self-triggered radial multi-index test strip device, relating to the field of immunochromatographic test strip technology. It features a circular, integrated snap-lock sealed structure, comprising, from top to bottom, a high-transparency top cover layer, a functional core layer, and a pressable base layer. The functional core layer integrates a concealed self-triggered blood collection module, a central negative pressure blood storage and dispensing module, and a radial multi-arm parallel test strip core. The pressable base layer includes a base mechanism and a pressing mechanism. The concealed self-triggered blood collection module is located at the center of the device and is linked to the pressing mechanism of the pressable base layer, enabling the needle to extend automatically upon puncture and retract immediately, generating negative pressure for automatic sample aspiration. The central negative pressure blood storage and dispensing module connects the concealed self-triggered blood collection module to the radial multi-arm parallel test strip core. Each testing arm can obtain sufficient samples, further improving the consistency and accuracy of the test results.

The present application belongs to the field of immune detection technology, and particularly relates to a colloidal gold immunochromatographic test strip for detecting antibodies of multiple species of Toxoplasma gondii, a preparation method and application thereof. The test strip uses Staphylococcus aureus protein A as a detection line, and can detect serum antibodies in various animals such as pigs, sheep, goats and cats. A DNP system is used as an independent quality control line, avoiding the dependence of a traditional quality control line on the spatial conformation of SAG1 protein, and improving the stability and repeatability of the test strip. The test strip is used in combination with a sample diluent to detect negative samples, and the negative coincidence rate reaches 100%. The test strip is used in combination with a sample diluent to detect clinical serum samples, and the coincidence rate with IFA reaches 97.6-100%, indicating that the test strip and the sample diluent have good diagnostic reliability, specificity and accuracy, are portable and simple to operate, and can determine the result by naked eye within 5 minutes without relying on professional equipment, and are suitable for on-site rapid detection and clinical detection in farms.

This invention belongs to the field of biotechnology and discloses a detectable and therapeutic antibody against the venom of the Chinese cobra and its uses. This invention provides an antibody or its antigen-binding fragment that can target and recognize different key toxin components or antigenic epitopes in venom, exhibiting excellent diagnostic specificity and therapeutic and preventative capabilities. Utilizing the high specificity of this antibody or its antigen-binding fragment in recognizing snake venom antigens, it can be developed into a rapid detection kit (such as a lateral flow immunochromatographic test strip) for rapid identification of the biting snake species and detection of the presence of specific snake venom in the environment or sample, providing crucial technical support for precise treatment and on-site diagnosis. It can also be used directly as a neutralizing agent for emergency treatment after snakebite. Through injection, the antibody can specifically neutralize the snake venom toxins entering the body, blocking its toxic effects and providing a novel and specific treatment method for clinical care.

A problem to be solved is to provide a test strip capable of avoiding occurrence of a so-called bleaching phenomenon in an immunochromatographic test strip to enable better detection and visibility. Another problem to be solved is to provide an immunochromatographic test strip capable of preventing a variation in detection value on a test line.The problems are solved by an immunochromatographic test strip including at least a porous membrane and having a sample application region, a development region, and a detection region in which a specific binding substance is immobilized, wherein the detection region is immobilized on a front surface of the porous membrane, and wherein an opaque base material having a thickness of 30 μm or more and 200 μm or less is placed on the back surface of the porous membrane.

This invention discloses a dual-color quantum dot fluorescent ball immunochromatographic test strip and its preparation method. The test strip's reaction pad is coated with a first antibody labeled with green quantum dot fluorescent balls and a second antibody labeled with red quantum dot fluorescent balls. The reaction pad is sequentially equipped with a T1 line, a T2 line, a C line, and a calibration line. The T1 line is coated with the first antibody, the T2 line with the second antibody, the C line with the secondary antibody, and the calibration line with yellow water-soluble quantum dots emitting a wavelength of λ3. This invention uses two quantum dot fluorescent balls of different particle sizes and colors as fluorescent markers for two different detection indicators, reducing the influence of cross-reactivity during multi-indicator detection in immunochromatographic assays and avoiding interference between fluorescent markers that occurs during simultaneous detection of multiple indicators.

The application belongs to the technical field of biological detection, and discloses a pig delta coronavirus lateral flow immunochromatographic detection test strip based on nanobodies and application thereof. The test strip takes PDCoV nucleocapsid protein as a detection target, adopts a sandwich type immunodetection principle, and the core recognition element is two high-affinity nanobodies recognizing different epitopes of PDCoV N protein. 62Nb is fused with a pig IgG Fc fragment to form 62Nb-pFc, and colloidal gold is coupled as a detection probe; 94Nb is fixed on the T line as a capture antibody, and the C line is fixed with a goat anti-pig IgG. The nanobodies are prepared by a Pichia pastoris secretion expression system. The test strip is simple to operate and does not require instruments, and can complete visual detection of PDCoV in complex samples such as pig fecal swabs and breeding environment wastewater in 10-15 minutes. The test strip has high sensitivity, strong specificity, good stability, good batch consistency, and is suitable for on-site screening and biological safety monitoring in breeding farms.

The present application relates to edible mushroom cultivation and biological detection technical field, more specifically, the present application relates to a kind of for tea tree mushroom substrate tox dead klab and fipronil rapid detection colloidal gold immunochromatographic test strip preparation method.The method is labeled with monoclonal antibody by EDC / NHS covalent coupling in carboxyl containing colored latex microspheres, resuspended after spotting and drying in coated micropore bottom;Sample pad is pretreated with buffer salt and tween, detection line and quality control line are set on nitrocellulose membrane and completed assembly cutting strip.Detection is carried out by pre-reaction in coated micropore before entering test strip chromatography color development, realize the rapid screening of tox dead klab and fipronil related target in tea tree mushroom substrate;Multiple detection lines or multiple micropores can be used to realize multiple target parallel detection, operation window is clear, and the result is stable.

The immunochromatographic test device of the present application includes: a loading section that detachably loads a kit including a carrier having a droplet application area to which a test object is applied and a detection area whose color development state changes depending on whether the test object is positive or negative; a photographing section that photographs the detection area; and a processor that performs determination of whether the test object is positive or negative, i.e., main determination, based on a detection area image of the detection area photographed by the photographing section. When a diffusion direction of the test object on the carrier is set as a row direction and a direction intersecting the row direction is set as a column direction, the detection area is a linear area extending in the column direction, the detection area image is an image in which a plurality of pixels are two-dimensionally arranged in a matrix shape, and the processor performs the main determination using remaining pixels obtained by excluding one or more high-concentration pixels having relatively high concentration within each column from the detection area image.

An immunochromatographic test strip (100) includes: a sample pad (101) into which a sample is introduced; and a detection unit (103) for detecting a substance to be detected in the sample. The detection unit (103) of the immunochromatographic test strip (100) carries: a proteinaceous or peptidic capture substance that specifically binds to the substance to be detected; and at least one sugar selected from a group consisting of sugars having three or more saccharide units and polysaccharides.

Compared to conventional immunochromatographic test kits, this kit reduces the amount of capture antibody used, providing a lower-cost immunochromatographic test kit. [Solution] The immunochromatographic test kit comprises a sample application section onto which the sample is dropped, a conjugate section on which a labeled antibody having the property of binding to the target substance in the sample is immobilized, and a detection section on which a capture antibody having the property of binding to the target substance is attached. The detection section is formed on a carrier and cannot be observed by visual inspection. The immunochromatographic test kit is designed to allow direct observation of the labeling substance of the labeled antibody bound to the target substance captured in the detection section using a microscope.

This invention discloses an immunochromatographic assay kit, preparation method, and application for the detection of apolipoprotein E4. The kit includes a test card and sample diluent. The test card comprises a test strip, which includes a PVC base, absorbent paper, a glass fiber pad, and a nitrocellulose membrane. The glass fiber pad is loaded with polystyrene latex microspheres for physical adsorption. The surface of the polystyrene latex microspheres is modified with sulfonyl or hydroxyl groups, and the zeta potential ranges from -15V to -20V. After the polystyrene latex microspheres are uniformly coated on a glass slide and dried, the contact angle between them and water droplets is greater than 90°. The nitrocellulose membrane is loaded with anti-APOE4 antibody, coating solution, and protein A. The sample diluent includes PBS, TWEEN-20, Tetronic 1307, and CHAPS. This invention eliminates the need for monoclonal antibody-labeled latex microspheres, reducing false positives and reagent batch-to-batch variation, and achieving higher sensitivity.

This invention discloses an immunochromatographic assay card, detection method, and application for broadening the quantitative detection range. The immunochromatographic assay card for broadening the quantitative detection range includes: a sample pad, a conjugation pad, a nitrocellulose membrane pad, and absorbent paper sequentially connected on a base plate; the nitrocellulose membrane pad includes a detection zone and a control zone; the detection zone is provided with a capture antibody detection line and an antigen detection line, and the control zone is provided with a control line; the conjugation pad is coated with a first label and a second label, the first label including a labeled antibody that binds to the target analyte, and the second label including a label that specifically binds to the capture reagent coated on the control line. This invention, by combining the sandwich method with a competitive method, effectively broadens the linear detection range, avoids the hook effect, and improves the accuracy and sensitivity of the detection, making it particularly suitable for quantitative detection of items with a wide concentration range.