136 results about "Immunochromatographic test" patented technology

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention discloses an immunochromatography test strip based on a nano-robot as well as a preparation method and application thereof. The immunochromatography test strip comprises a bottom plate, and a sample pad, filter paper, a nitrocellulose membrane and absorbent paper which are sequentially overlapped and adhered to the bottom plate, when in use, an FPSN (at) Ab buffer solution is dropwise added on the surface of the sample pad; the FPSN (at) Ab buffer solution is prepared from Fe3O4 (at) mPDAamp; the preparation method comprises the following steps: preparing an mSiO2 (at) Nanozyme nano composite material and an antibody to be marked; the Fe3O4 (at) is mPDAamp; the mSiO2 (at) Nanozyme nano composite material is nano particles with an asymmetric structure, one end of the asymmetric structure is a core-shell structure, the core is Fe3O4, the shell is mesoporous polydopamine particles, the other end is rod-like mesoporous SiO2, and noble metal nano particles are loaded on the surfaces of Fe3O4 and mesoporous SiO2; according to the invention, the nano-robot is used as the probe to construct the immunochromatography test strip, so that the detection sensitivity and the detection speed of the test strip are improved, the binding efficiency between the probe and the to-be-detected object is optimized, and the problem that in the prior art, accurate detection of disease markers is difficult to realize under the conditions of small sample size and low concentration is solved.

The invention relates to the technical field of immunochromatography, in particular to time-resolved fluorescent microspheres, a preparation method and application of the time-resolved fluorescent microspheres in PSP / CRP / MxA quantitative detection immunochromatography test strip, and the preparation method of the time-resolved fluorescent microspheres comprises the following steps: 1) preparing surface aminated polystyrene microspheres; (2) carrying out glutaraldehyde crosslinking and 1, 10-phenanthroline-5-amino combination; 3) grafting a rare earth fluorescent dye on the surface; and 4) coating a polymer shell layer and modifying carboxyl. According to the invention, through a dual fixation strategy of surface grafting and polymer coating, the time-resolved fluorescent dye is confined to a functional region between the surface of the microsphere and a polymer shell layer, diffusion and leakage of dye molecules to the external environment are blocked, and the problem of dye shedding of traditional fluorescent microspheres is solved; the polymer shell layer can effectively isolate external ultraviolet light, oxygen and other corrosive factors, reduce photooxidative degradation and non-radiative transition loss of fluorescent molecules, and improve the light stability and fluorescent lifetime of the fluorescent molecules.

The invention discloses an anti-rabies virus N protein monoclonal antibody, a detection reagent and application of the anti-rabies virus N protein monoclonal antibody. The monoclonal antibody for resisting the rabies virus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with relatively high affinity and detection sensitivity for resisting rabies virus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

This invention provides an assembled high-throughput immunochromatographic test strip array sample application device, comprising a 96-well plate fixing bracket (1) and a test strip support plate (2); immunochromatographic test strips (3) are arranged and fixed by slots (4) and limiting blocks (5) on the test strip support plate (2), with their sample application areas (6) protruding from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device. This device can be used for high-throughput sample application in immunochromatographic detection.

The invention discloses an antibody for detecting porcine acute diarrhea syndrome coronavirus, a fluorescence immunochromatography test strip and application of the antibody and the fluorescence immunochromatography test strip, and aims to overcome the defects that an existing SADS-CoV detection technology is low in sensitivity, complex to operate and incapable of being applied on site. The method comprises the following steps: screening high-specificity anti-S1 protein monoclonal antibodies (such as 1B8 and 3C9); a quantum dot labeled antibody is combined with a chromatographic structure containing a T line (S1 protein) and a C line (SPA protein); the kit provided by the invention has the advantages that by optimizing the formula of the buffer solution, the technical effects of high-sensitivity detection (100 TCID50 / mL), on-site rapid visual interpretation and zero cross reaction are realized, the technical bottleneck of on-site rapid and accurate diagnosis is overcome, the operation is simple and convenient, and the kit is suitable for on-site screening of clinical samples.

The utility model is designed for the rapid (up to 20 minutes) detection of at least 10 types of analytes in environmental samples (water, air, soil, etc.) and clinical specimens. The immunochromatographic test strip for multiplex analysis includes a polymer substrate with an applied immunochromatographic membrane and a capture pad. The test and control zones of the test strip are designed as two rows of transverse microstrips occupying no more than half the width of the test strip. The test strip includes a marker for coordinating the cutting of test strips. The technical result consists in reducing the time for recording the luminescent signal from test strips in an automated system. 3 c.p. phyl., 3 fig., 2 tables.

The application discloses an anti-LMBV single-chain antibody and application thereof in preparation of a quantum dot fluorescent immunochromatography test strip, and belongs to the technical field of biological detection. The single-chain antibody comprises Ab-K1 and Ab-K2, the amino acid sequence of the Ab-K1 is shown as SEQ ID NO. 11, and the amino acid sequence of the Ab-K2 is shown as SEQ ID NO. 12. The single-chain antibody used in the application can accurately recognize and combine with iridovirus in a sample to be detected, so that the accuracy of detection is ensured. Meanwhile, the antibody labeled with fluorescent quantum dots has good fluorescent characteristics, so that the fluorescent signal is easy to observe and detect, and the specific surface area is relatively large, so that the antibody can be combined with more virus molecules, so that the sensitivity of detection is improved, the accuracy and sensitivity of detection are improved, the operation steps are simplified, and powerful technical support is provided for detection and prevention and control of iridovirus.

The application provides a quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids and an application thereof, and belongs to the technical field of veterinary biological diagnosis products. The quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids comprises a base plate, on the base plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are sequentially connected in a head-to-tail mode, from the combination pad to the water absorption pad, the nitrocellulose membrane is sequentially provided with an antibody detection line, a nucleic acid detection line and a quality control line, the antibody detection line is coated with a goat anti-pig IgG-Fc antibody, the nucleic acid detection line is coated with streptavidin, and the quality control line is coated with a rabbit anti-sheep IgG antibody; the combination pad is coated with a quantum dot microsphere labeled recombinant protein P22 and a quantum dot microsphere labeled sheep anti-digoxin antibody. The test paper can rapidly, highly specifically and highly sensitively detect ASFV antibodies and nucleic acids.

The application discloses a lateral flow immunochromatography test paper based on enzyme / magnetic driving magnetic gold nano probes. First, a magnetic gold nano composite material (Fe3O4@PDA@AuNS, MPAuNS) with a core (Fe3O4)-shell (PDA) structure and modified with gold nano stars (AuNS) on the surface is synthesized, and the magnetic gold nano composite material is applied to lateral flow immunochromatography cardiac troponin I (cTnI) detection as a nano probe. The enzyme / magnetic driving refers to: 1) the glucose oxidase activity of the gold nano star is used to drive the diffusion enhancement of the nano probe by catalytically decomposing glucose in the cTnI solution; and 2) the magnetic response characteristics of the Fe3O4 core are used to realize the magnetic driving movement of the nano probe by placing a magnet on the absorption pad through magnetic attraction. The probe can improve the speed and sensitivity of lateral flow immunochromatography detection to a certain extent.

The utility model relates to the technical field of manufacturing of auxiliary equipment for boxing and packaging finished immunochromatography test paper products, in particular to a quantitative product packaging device which is reasonable in structure, reliable in work and capable of quantitatively adjusting the packaging quantity according to requirements, and is characterized by being provided with a hopper, a product grabbing mechanism, a product conveying mechanism and a counting mechanism, a material taking opening is formed in the hopper, the product grabbing mechanism and the material taking opening are oppositely arranged, the hopper and the product grabbing mechanism are arranged above the product conveying mechanism, and a negative pressure material taking suction cup and a linear piston motion air cylinder used for driving the negative pressure material taking suction cup to be close to / away from the material taking opening of the hopper are arranged in the product grabbing mechanism. The negative pressure material taking suction cup is driven by the linear piston motion air cylinder to suck materials at the material taking opening and then is driven by the linear piston motion air cylinder to leave the material taking opening, negative pressure is relieved, and the grabbed materials are put onto the product conveying mechanism.

The invention provides an immunochromatography test strip for detecting clobetasol propionate in cosmetics and a method for quantitatively detecting clobetasol propionate in cosmetics. According to the invention, a hollow gold nano-labeled material is applied to immunodetection of glucocorticoid clobetasol propionate for the first time, and an immunochromatography test strip with high sensitivity, quantitative function and strong specificity is constructed. The test strip can complete detection within 15 minutes, has high detection sensitivity, and realizes accurate quantification in a range of 0.01-25 ng / mL; meanwhile, the method is high in anti-interference capacity, the detection recovery rate of actual cosmetic samples reaches up to 89%-107%, the false positive rate and the false negative rate are both 0, reliability is good, and a powerful screening analysis tool is provided for rapid screening and quantitative detection of clobetasol propionate addition in cosmetics.

The application discloses a microbial nano-enzyme probe, a method for detecting salbutamol and application, and comprises a salbutamol monoclonal antibody and a signal carrier, wherein the signal carrier is a yeast cell loaded with iridium oxide nanoparticles. The application firstly constructs a probe by using a yeast cell loaded with iridium oxide nanoparticles in the detection of an immunochromatographic test strip, uses [3, 3', 5, 5' tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2)] as a substrate to trigger a color developing reaction, can realize high-sensitivity target detection, and the detection limit is as low as 0.012 ng / mL, which is 55 times higher than the sensitivity of a traditional gold-labeled test strip. In addition, the application has a good application prospect, can provide a universal platform for sensitive, accurate and rapid detection of food-borne stimulants, and can be successfully applied to the detection of salbutamol in pig liver and beef samples.

The invention discloses an immunochromatography test strip based on an asymmetric near-infrared driving nano motor and a preparation method and application thereof.The immunochromatography test strip comprises a bottom plate and a sample pad, filter paper, a nitrocellulose membrane and absorbent paper which are sequentially overlapped and pasted to the bottom plate, and when the immunochromatography test strip is used, a PZSA-Ab buffer solution is dropwise added to the surface of the sample pad; the PZSA (at) Ab buffer solution is prepared from a PS-SiO2 (at) Au nano composite material and an antibody to be marked; the PS-SiO2 (at) Au nano composite material is nanoparticles of an asymmetric snowman-shaped structure, polystyrene nanospheres are arranged at one end of the asymmetric snowman-shaped structure, hemispherical SiO2 nanoparticles are arranged at the other end of the asymmetric snowman-shaped structure, and Au nanoparticles are loaded on the surface of the asymmetric snowman-shaped structure. According to the present invention, the PS-SiO2 (at) Au nano-composite material is adopted as the nano-motor to be combined with the antibody to be labeled, and the near-infrared light is adopted to drive the snowman-shaped nano-motor to directionally move so as to achieve the active capture and enrichment of the tumor marker, such that the detection sensitivity and the detection speed of the immunochromatography test strip can be significantly improved.

The invention belongs to the technical field of medical supplies, and discloses a weakly ionized colloidal gold immunochromatography test strip based on a cationic polymer as a quality control line and a preparation method thereof. The weak ionized colloidal gold immunochromatography test strip comprises a substrate, a nitrocellulose membrane, a combination pad and a water absorption pad, the nitrocellulose membrane, the combination pad and the water absorption pad are all distributed on the surface of the substrate; the combination pad is lapped on the nitrocellulose membrane, the water absorption pad is lapped on the nitrocellulose membrane, and the combination pad and the water absorption pad are distributed on two sides of the nitrocellulose membrane; a detection line and a quality control line are arranged on the nitrocellulose membrane; the quality control line is coated with poly dimethyl diallyl ammonium chloride; the weak ionized colloidal gold immunochromatography test strip further comprises a CEA monoclonal antibody Ab1-weak ionized colloidal gold compound. Directional charge adsorption synergy is formed through weak ionized colloidal gold and a PDDA quality control line with strong positive electricity, and gt is still kept in a high-salt environment; the capture stability is 95%, and the production cost of the C line is reduced by 90%.

The invention discloses a monoclonal antibody for resisting canine coronavirus N protein, a detection reagent and application of the monoclonal antibody and the detection reagent. The monoclonal antibody for resisting the canine coronavirus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with high affinity and detection sensitivity to canine coronavirus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention provides spread immunochromatography test paper and a preparation method thereof. The immunochromatography test paper comprises a sample pad, a marking pad and an immunochromatography membrane, at least one of the sample pad, the marking pad and / or the immunochromatography membrane comprises a surfactant, and the surfactant is used for reducing the surface tension of a solution to be lower than 25 mN / m, preferably lower than 22 mN / m, and further preferably lower than 20 mN / m, so that the hyper-spreading of the solution is promoted. Compared with the prior art, the immunochromatography test paper provided by the invention has the advantages that the detection speed is higher, the consumed time is shorter, and the working efficiency of workers in the field can be greatly improved. And the signal strength is also accidentally improved.

This invention belongs to the field of molecular biology detection technology, specifically relating to a Nipah virus (NiV) nucleic acid detection kit combining RT-LAMP and immunochromatography, and its application. This kit combines the high sensitivity and specificity of loop-mediated isothermal amplification (LAMP) technology with the rapid and visual interpretation advantages of immunochromatographic test strips, enabling on-site, rapid, and accurate detection of NiV. The core components of this invention include: a set of specific primers designed for conserved regions of the NiV gene, an optimized RT-LAMP reaction system, and specially treated immunochromatographic test strips for detecting LAMP amplification products. This kit eliminates the need for reverse transcription of RNA virus nucleic acids and expensive thermal cycling equipment. The entire detection process is completed within 20–30 minutes, with a sensitivity of up to 300 copies / μL. This solution combines the high efficiency of RT-LAMP technology with an intuitive, equipment-free detection method, achieving truly rapid and accurate on-site diagnosis of NiV.

The present application relates to the technical field of antibody detection, in particular to a TGEV IgA antibody fluorescent microsphere immunochromatography test strip and preparation and application thereof.The TGEV IgA antibody fluorescent microsphere immunochromatography test strip comprises a base plate, and a sample pad, a nitrocellulose membrane and a water absorption pad which are sequentially and lapped and pasted on the base plate; the sample pad is a glass fiber membrane, and the glass fiber membrane is coated with a fluorescent microsphere labeled TGEV-S1 recombinant protein conjugate and a fluorescent microsphere labeled rabbit IgG antibody conjugate; the nitrocellulose membrane is provided with a detection line and a quality control line, wherein the detection line is coated with a goat anti-pig IgA antibody, and the quality control line is coated with a goat anti-rabbit IgG antibody.The test strip can accurately capture low-titer TGEV IgA antibodies, is suitable for high-throughput rapid on-site detection, and has the characteristics of low reagent cost and detection difficulty, high sensitivity and high specificity.

The invention provides high-specificity immunochromatography test paper as well as a preparation method and application thereof, and relates to the technical field of biological detection, the high-specificity immunochromatography test paper sequentially comprises a sample pad, a blood filtering pad and a combination pad which are lapped on a bottom plate in the chromatography direction; the blood filtering pad contains a pretreatment reagent, the pretreatment reagent comprises an anti-human RBC antibody, Tween-20 and bovine serum albumin, a blood filtering pad assembly is added between the combination pad and the detection pad of the immunochromatography test paper, and the blood filtering pad contains the pretreatment reagent, namely the anti-human RBC antibody, Tween-20 and bovine serum albumin, so that a sample is filtered to remove interfering substances; the reaction is homogenized; data show that the membrane surface background is free of redness, clean and free of interference in blood screen item detection, high specificity of products is guaranteed, and blocking agents are not used. The technical problem that nonspecific interference of an immunoreaction inhibitor is generated by using a blocking agent in immunochromatography test paper in the prior art is solved.

The invention relates to the technical field of detection, and discloses an immunochromatography test strip, a kit and application of the immunochromatography test strip, the immunochromatography test strip comprises a chromatography membrane, and a protein compound formed by mixing and incubating a recombinant protein A dimer and a capture antibody is fixed on a detection line of the chromatography membrane. The double-antibody sandwich immunochromatography detection method has the advantages that the high-efficiency capture of antigens is realized, the detection performance of double-antibody sandwich immunochromatography is enhanced, the sensitivity of the detection method can be improved, the implementation is simple and convenient, and the method can be applied to the development of the double-antibody sandwich immunochromatography method for various detection targets.

The invention relates to the technical field of biological detection, and particularly discloses a preparation method and application of a Brucella antibody immunochromatography detection test strip based on colloidal carbon. The Brucella antibody immunochromatography detection test strip provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; the binding pad contains lipopolysaccharide of colloidal carbon labeled brucella, the nitrocellulose membrane is provided with brucella lipopolysaccharide and a goat anti-mouse igG antibody which are respectively used as a detection line (T) and a quality control line (C), and the colloidal carbon technology-based immunochromatography test paper is established by detecting different specimens and comparing with other detection methods. The test strip prepared by the invention has the advantages of no subjective influence of a detector, simplicity and convenience in operation, high detection sensitivity, strong specificity and the like.

The invention discloses an anti-rabies virus G protein monoclonal antibody, a detection reagent and application of the anti-rabies virus G protein monoclonal antibody and the detection reagent. The anti-rabies virus G protein monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody provided by the invention has high affinity and detection sensitivity to rabies virus G protein, and lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The present invention relates to an immunochromatographic test strip (1) which can be encoded such that the test result cannot be understood by the user who performs the test. The test strip (1) subject of invention, includes at least one sample pad (2) in which the samples are loaded, at least one conjugate pad (3) having analyte-specific labeled antibodies present in the sample, at least one membrane (4) wherein the antibodies bound or unbound to the analyte moves forward and at least one absorption pad (5) providing movement of the sample and a two-dimensional barcode (6) on the membrane (4) that provides the authorities to interpret the results and avoids one who takes test image after test so that the test taker does not immediately understand the test result.

The application discloses a signal reading method, system, device and medium for a multi-target array test strip, and relates to the technical field of fluorescent immunochromatographic detection. The method collects an RGB fluorescent image of the multi-target array test strip; a binary image is obtained through grayscale processing and adaptive threshold segmentation; an effective signal contour is extracted; a spatial position calibration and automatic sorting of discrete fluorescent signals are performed through an intelligent sorting algorithm combining DBSCAN clustering and principal component analysis; non-uniform background interference is removed through a robust local regression method to obtain a purified signal; and finally, real RG ratio signal values of each target point are extracted. The application solves the problems of difficulty in multi-target positioning and incomplete background removal of the existing array test strip signal reading method through an intelligent algorithm, realizes accurate positioning, automatic sorting and efficient purification of multi-target fluorescent signals, and significantly improves detection accuracy. The application is suitable for quantitative analysis of multi-target immunochromatographic test strips.

The invention belongs to the technical field of biological detection, and discloses a rapid immunochromatography detection kit for gastrointestinal pathogenic bacteria based on a viral nano-enzyme probe. The invention discloses a nano-enzyme probe, the virus-shaped nano-enzyme probe provided by the invention takes Fe3O4 nano-particles as a core, Pt nano-particles and Au (at) Pt nano-particles are coated in sequence, a specific antibody is modified, and the nano-enzyme probe has a magnetic enrichment effect and a multi-catalytic-site characteristic at the same time; rapid capture and high-sensitivity quantitative detection of various gastrointestinal pathogenic bacteria in complex matrixes (river water samples, lake water samples and excrement samples) can be realized, and matrix interference in practical application is eliminated; and the kit has huge potential in the aspects of high-sensitivity field screening and clinical monitoring of infection of gastrointestinal bacteria (such as escherichia coli, salmonella and helicobacter pylori).

This invention provides a fully automated immunoassay analyzer, comprising: a base, a reaction disk for carrying multiple immunochromatographic test strips installed inside the base, a sample processing area for processing samples installed on the base, and a moving arm assembly movably installed on the base for driving a pipette arm to perform liquid pipetting, mixing, and spotting operations in the sample processing area. Compared with the prior art, this invention has the following advantages: by setting up the moving arm assembly, in conjunction with the pipette arm and negative pressure nozzle, its automated operation can simultaneously perform multiple sample single detection, single sample multiple detection, or multiple sample multiple detection, with high throughput, making up for the insufficient throughput of existing manual experiments. Automated sample pretreatment and automatic recycling of waste after experiments avoid manual errors and are convenient and pollution-free.

The invention relates to the technical field of in-vitro detection, in particular to a portable device for AQP4-IgG instant detection and a preparation method and application of the portable device. The portable device for the instant detection of the AQP4-IgG comprises a lateral flow immunochromatography test strip, a detection line of a reaction pad of the lateral flow immunochromatography test strip is coated with gelling cells over-expressing AQP4 protein. The confining liquid is used for mixed incubation with a to-be-detected sample, and the confining liquid is dispersion liquid of gelatinized cells. And the back surface of the position of the detection line of the reaction pad is modified with Nafion-CNF hybrid hydrogel. According to the technical scheme, the technical problem that a rapid, accurate and low-cost AQP4-IgG detection method is lacked in the prior art can be solved. On the basis of cell transfection, intracellular gelling and lateral flow immunochromatography technologies, detection can be completed within 10 min, the detection limit is as low as 2 ng / mL, and the kit has ideal application prospects.

A problem to be solved is to provide a test strip capable of avoiding occurrence of a so-called bleaching phenomenon in an immunochromatographic test strip to enable better detection and visibility. Another problem to be solved is to provide an immunochromatographic test strip capable of preventing a variation in detection value on a test line.The problems are solved by an immunochromatographic test strip including at least a porous membrane and having a sample application region, a development region, and a detection region in which a specific binding substance is immobilized, wherein the detection region is immobilized on a front surface of the porous membrane, and wherein an opaque base material having a thickness of 30 μm or more and 200 μm or less is placed on the back surface of the porous membrane.