256 results about "Immunochromatographic test" patented technology

The present invention belongs to the field of biological detection technology, specifically relating to a monoclonal antibody combination for detecting HPV18 E7 protein and its application. This combination consists of monoclonal antibodies 5F8 and 6G6, wherein the heavy and light chain variable regions of 5F8 and 6G6, respectively, contain specific complementarity-determining region sequences (as shown in SEQ ID NOs. 1 to 12), conferring high specificity for HPV18 E7 protein. Verification using a double-antibody sandwich ELISA and a biotin-avidin amplification system demonstrated that this antibody combination specifically recognizes HPV18 E7 protein, exhibits no cross-reactivity with other high-risk HPV oncoproteins, and exhibits a detection sensitivity of 1 ng / mL. This combination can be used to construct detection systems such as ELISA kits and immunochromatographic test strips, suitable for rapid screening of HPV18 E7 protein in cervical exfoliated cell samples.

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The method comprises the following steps: collecting a group of raw milk submission test information table images, and intercepting five types of bigeminy colloidal gold test paper on each submission test information table as a to-be-detected data set; replacing a C2f module of the YOLOv8 backbone network with an irregular feature extraction module, adding a position information attention module at the tail of the backbone network, and constructing to obtain a test paper color development recognition model; training the test paper color development recognition model based on an improved WIoU loss function and a self-defined sample set containing different color development conditions; inputting the to-be-detected data set into a trained test paper color development recognition model for recognition to obtain a plurality of prediction frames; and determining a regional color development recognition result by comparing the sizes of the projections accumulated by the pixel values on the same vertical coordinates of the plurality of prediction frames. According to the invention, a target detection technology is applied to an immunochromatography test paper color development recognition task, and a new thought and a solution are provided for color development recognition of batch immunochromatography test paper.

The invention relates to the technical field of in-vitro diagnostic kits, and provides an apolipoprotein E4 detection kit and application thereof. The immunochromatography test paper comprises detection immunochromatography test paper, the detection immunochromatography test paper comprises a bottom plate, a sample pad, a combination pad, an NC membrane and a water absorption pad, the combination pad and the sample pad are sequentially arranged on one side of the NC membrane, and the water absorption pad is arranged on the other side of the NC membrane; the combination pad is marked with gold manganese oxide nanometer developing particles and an apolipoprotein APOE4 monoclonal antibody. According to the technical scheme, the problems of complicated operation, high cost, low detection sensitivity and weak specificity of apolipoprotein E4 detection in related technologies are solved.

The invention provides a reagent for detecting avian leukosis virus, a nano-enzyme immunochromatography test strip and application of the nano-enzyme immunochromatography test strip, and belongs to the technical field of immunology. Aiming at the problems that the existing avian leukosis virus detection method is complicated in operation, time-consuming or strong in equipment dependence, the reagent comprises a capture antibody and a detection antibody; a heavy chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 1, and a light chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 2; a heavy chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 5, and a light chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 6; the antigen binding fragment is a Fab fragment, an F (ab) 2 fragment or a single-chain Fv fragment. The reagent is mainly used for detecting avian leukosis virus, and is suitable for detecting samples such as cloaca swab, meconium, egg white or serum of poultry.

The invention provides an immunochromatographic test strip for quantitatively detecting monkey pox virus based on fluorescent latex microspheres, which takes europium chelate Eu (DBM) 3Phen-loaded monodisperse carboxylated polystyrene copolymer microspheres as a signal substance, and the microspheres are subjected to acid-base treatment before loading to loosen the surface structure of the microspheres so as to improve the loading capacity; the surface compound of the test strip is prepared by coupling carboxyl on the surface of the microsphere with an antibody. The invention also discloses a preparation method of the test strip. The kit is convenient for rapidly and accurately detecting the monkey pox virus, and has the characteristics of simplicity, rapidness, no toxicity and low cost.

The invention belongs to the technical field of colloidal gold immunochromatography test strips, and particularly relates to an enhanced colloidal gold immunochromatography test strip pretreatment solution and a preparation method thereof.The pretreatment solution comprises a sample pad pretreatment solution and a conjugate pad pretreatment solution, and the sample pad pretreatment solution comprises trehalose, Tween 20, BSA, a Proclin300 preservative and a borate buffer solution; the conjugate pad pretreatment solution comprises trehalose, Tween 20, BSA, PEG20000, a Proclin300 preservative, polyvinyl alcohol, chitosan and a borate buffer solution, and the enhanced colloidal gold immunochromatography test strip pretreatment solution can enhance the binding force between an antibody on the conjugate pad and colloidal gold while improving the permeability and diffusivity of a sample pad, and maximally maintain the activity of the antibody; and the detection sensitivity and accuracy are always kept at excellent levels, so that the overall performance of a colloidal gold immunochromatography detection product is improved.

The invention relates to the technical field of biological detection, and particularly discloses a bovine brucella antibody immunochromatography detection test strip as well as a preparation method and application thereof. The bovine brucella antibody immunochromatography detection test strip provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; the combination pad contains a mouse anti-bovine IgG monoclonal antibody marked by quantum dot microspheres; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a brucella LPS antigen, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The bovine brucella antibody immunochromatography detection test strip provided by the invention has the advantages of no subjective influence of a detector, simplicity and convenience in operation, high detection sensitivity and specificity and the like.

The invention provides an immunochromatography kit and an immunochromatography test strip detection method for ricin, and belongs to the technical field of toxin detection. The method comprises the following steps: mixing and incubating an oligonucleotide chain substrate labeled with a fluorophore and biotin, a buffer solution, a BSA solution and a sample to be detected, and adding ammonia water to terminate the reaction to obtain a reaction solution; adding the reaction solution to an immunochromatography test strip, incubating in a dark place, detecting a fluorescence signal value, and determining whether ricin exists according to a difference value between a final sample fluorescence signal value and a final fluorescence signal value of negative control; the sample to be detected is enriched by using antibody-coated magnetic beads; if the fluorescence signal value of the sample is lower than the negative control fluorescence signal value-3 times of the standard deviation, it is judged that ricin exists. No cross reaction exists between the detection method and an abrus precatorius toxin sample, and the detection sensitivity can reach 0.17 ng / mL. When the kit is applied to detection of a milk simulation sample, the sensitivity can reach 1.53 ng / mL.

The invention discloses a portable immunochromatography device for detecting an African swine fever virus neutralizing antibody and application thereof.The device comprises a reading module, a sample preparation module and a detection module, the sample preparation module and the detection module are integrally connected up and down, and the sample preparation module is sequentially provided with a sample adding hole, a filtering chromatography cavity and a communicating pipe which are communicated from top to bottom; a filtering chromatography part is arranged in the filtering chromatography cavity, the detection module is provided with a fluorescence immunochromatography test strip and a shell, the fluorescence immunochromatography test strip is located in the shell, a sample pad, a fluorescence marker pad, an NC membrane and a water absorption pad of the fluorescence immunochromatography test strip are sequentially overlapped and adhered to a PVC bottom plate, and the two ends of a conduction pipe are communicated with the filtering chromatography cavity and the shell respectively. One end of the fluorescence immunochromatography test strip is located below the filtration chromatography component, the other end of the fluorescence immunochromatography test strip is located above the sample pad, the water absorption pad of the fluorescence immunochromatography test strip and the NC membrane are sequentially inserted into the reading module, and the reading module is connected with the shell through a buckle and reads a quality control line and a detection line of the inserted NC membrane.

The invention discloses an immunochromatography test strip based on a nano-robot as well as a preparation method and application thereof. The immunochromatography test strip comprises a bottom plate, and a sample pad, filter paper, a nitrocellulose membrane and absorbent paper which are sequentially overlapped and adhered to the bottom plate, when in use, an FPSN (at) Ab buffer solution is dropwise added on the surface of the sample pad; the FPSN (at) Ab buffer solution is prepared from Fe3O4 (at) mPDAamp; the preparation method comprises the following steps: preparing an mSiO2 (at) Nanozyme nano composite material and an antibody to be marked; the Fe3O4 (at) is mPDAamp; the mSiO2 (at) Nanozyme nano composite material is nano particles with an asymmetric structure, one end of the asymmetric structure is a core-shell structure, the core is Fe3O4, the shell is mesoporous polydopamine particles, the other end is rod-like mesoporous SiO2, and noble metal nano particles are loaded on the surfaces of Fe3O4 and mesoporous SiO2; according to the invention, the nano-robot is used as the probe to construct the immunochromatography test strip, so that the detection sensitivity and the detection speed of the test strip are improved, the binding efficiency between the probe and the to-be-detected object is optimized, and the problem that in the prior art, accurate detection of disease markers is difficult to realize under the conditions of small sample size and low concentration is solved.

The invention relates to the technical field of immunochromatography, in particular to time-resolved fluorescent microspheres, a preparation method and application of the time-resolved fluorescent microspheres in PSP / CRP / MxA quantitative detection immunochromatography test strip, and the preparation method of the time-resolved fluorescent microspheres comprises the following steps: 1) preparing surface aminated polystyrene microspheres; (2) carrying out glutaraldehyde crosslinking and 1, 10-phenanthroline-5-amino combination; 3) grafting a rare earth fluorescent dye on the surface; and 4) coating a polymer shell layer and modifying carboxyl. According to the invention, through a dual fixation strategy of surface grafting and polymer coating, the time-resolved fluorescent dye is confined to a functional region between the surface of the microsphere and a polymer shell layer, diffusion and leakage of dye molecules to the external environment are blocked, and the problem of dye shedding of traditional fluorescent microspheres is solved; the polymer shell layer can effectively isolate external ultraviolet light, oxygen and other corrosive factors, reduce photooxidative degradation and non-radiative transition loss of fluorescent molecules, and improve the light stability and fluorescent lifetime of the fluorescent molecules.

The invention provides a colloidal gold immunochromatographic test strip for detecting enramycin, a preparation method, and an application thereof, which belongs to the field of rapid immunoassay; the test strip comprises a backing plate, a sample pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad; the conjugate pad was fixed with colloidal gold-labeled enramycin monoclonal antibody; the nitrocellulose membrane is equipped with a T-line coated with enramycin artificial antigens and a C-line coated with goat anti-mouse secondary antibodies. The invention also provides a kit for detecting enramycin, comprising a test strip, a sample treatment solution, and a sample diluent. The invention further adds a test strip shooting background board to the kit, which can be used for rapid qualitative or quantitative detection of enramycin in animal feed and products of animal meat and egg with a detection limit of 10 mg / k

The invention belongs to the technical field of pesticide residue detection, and particularly relates to an SERS (Surface Enhanced Raman Scattering)-based multi-pesticide joint detection immunochromatography method as well as SERS immunochromatography test paper and an SERS immunoprobe used by the method. According to the invention, a surface enhanced Raman spectroscopy (SERS) technology is utilized, so that a detection signal is obviously enhanced, the detection sensitivity of pesticide residues is improved, and the pesticide residues with extremely low concentration can be detected. According to the present invention, with the combination of the immunochromatography (ICA) and the SERS technology, the pesticide types are distinguished by using different Raman internal standards, such that a variety of different pesticide residues can be simultaneously detected on the same test strip so as to improve the detection efficiency and the detection convenience. By means of high-specificity antibody reaction of immunochromatography, specific pesticide residues can be accurately recognized and detected, and the false alarm rate and the false positive rate are reduced. And the detection process is simple and rapid, does not need complex pretreatment steps, and is suitable for on-site rapid detection and high-throughput screening.

The invention discloses a preparation method and application of a colloidal gold immunochromatography test strip for detecting IDH1 mutant protein, and belongs to the field of rapid immunodetection. The colloidal gold chromatography test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane, a water absorption pad and colloidal gold, the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C in the flowing direction of a sample, the detection line T is coated with IDH1 mutant protein, and the quality control line C is coated with a second antibody corresponding to a monoclonal antibody of the IDH1 mutant protein. Clinical tumor tissue samples collected in real time in glioma resection surgery are treated, an object to be detected and an immune gold label are mixed, incubated and dropped in a test strip sample adding hole, and the object to be detected runs along the test strip under the driving of a buffer solution and generates visual color change. The test strip has the advantages of being rapid in reaction, easy and convenient to operate, convenient to carry, high in specificity and the like, makes up the blank of an IDH1 mutant protein concentration quantitative method, and has good clinical application value.

The invention discloses an immunochromatography test strip and a kit for rapidly detecting human urinary nuclear matrix protein 22, the test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are connected in sequence, the nitrocellulose membrane is provided with a detection line and a quality control line in sequence, the detection line is coated with an NMP22 antibody treated by a blocking agent, and the quality control line is coated with a quality control line. The quality control line is coated with a DNP antigen treated by a blocking agent, and the blocking agent comprises the following components in percentage by mass: 1% of an anti-heterotropism antibody agent, 0.2 mg / mL of an anti-rheumatoid factor agent, 1% of an anti-HAMA agent, 0.02 mol / L of PBS, 5% of sucrose and 0.1% of PC300. The immunochromatography test strip for rapidly detecting the human urinary nuclear matrix protein 22 is high in detection speed, capable of obtaining a detection result within 1-15 min, free of instruments, easy to operate, low in price, suitable for basic medical institutions and suitable for popularization and self-detection.

The invention provides a kit for detecting drug-resistant mutation of mycoplasma pneumoniae based on RPA-CRISPR and application of the kit. The invention provides an RPA primer pair and a crRNA sequence for detecting the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene, an RPA-CRISPR / Cas13a detection system is established, the detection system formed by the primer pair and the crRNA can accurately detect the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene within 30-40 minutes under an isothermal condition, the sensitivity and the specificity are very high, and the detection system can be used for detecting the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene. Amplification detection can be completed only by performing isothermal amplification at the temperature of 39 DEG C without using a variable-temperature instrument like PCR (Polymerase Chain Reaction); in addition, a lateral flow immunochromatography test strip is further combined, so that the on-site instant detection of the mycoplasma pneumoniae drug-resistant mutation can be realized.

A nanobody classical swine fever virus (CSFV)-E0-Nb1 against a CSFV E0 protein, and an encoding gene and use thereof are provided, belonging to the technical field of biological detection. The nanobody CSFV-E0-Nb1 has an amino acid sequence shown in SEQ ID NO: 1 and can be expressed using an expression system. The nanobody is coupled with a quantum dot to obtain an immunochromatographic test strip for distinguishing antibodies against a CSFV E2 subunit vaccine strain from those of a wild strain infected on site, and there is a simple production process of the test strip. The immunochromatographic test strip can differentiate and diagnose the antibodies against the CSFV E2 subunit vaccine strain and the wild strain, and has the advantages of rapid, convenient, and instant detection, thus providing a new method for the detection of classical swine fever (CSF) purification.

The invention discloses an anti-rabies virus N protein monoclonal antibody, a detection reagent and application of the anti-rabies virus N protein monoclonal antibody. The monoclonal antibody for resisting the rabies virus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with relatively high affinity and detection sensitivity for resisting rabies virus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

This invention provides an assembled high-throughput immunochromatographic test strip array sample application device, comprising a 96-well plate fixing bracket (1) and a test strip support plate (2); immunochromatographic test strips (3) are arranged and fixed by slots (4) and limiting blocks (5) on the test strip support plate (2), with their sample application areas (6) protruding from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device. This device can be used for high-throughput sample application in immunochromatographic detection.

The invention discloses an antibody for detecting porcine acute diarrhea syndrome coronavirus, a fluorescence immunochromatography test strip and application of the antibody and the fluorescence immunochromatography test strip, and aims to overcome the defects that an existing SADS-CoV detection technology is low in sensitivity, complex to operate and incapable of being applied on site. The method comprises the following steps: screening high-specificity anti-S1 protein monoclonal antibodies (such as 1B8 and 3C9); a quantum dot labeled antibody is combined with a chromatographic structure containing a T line (S1 protein) and a C line (SPA protein); the kit provided by the invention has the advantages that by optimizing the formula of the buffer solution, the technical effects of high-sensitivity detection (100 TCID50 / mL), on-site rapid visual interpretation and zero cross reaction are realized, the technical bottleneck of on-site rapid and accurate diagnosis is overcome, the operation is simple and convenient, and the kit is suitable for on-site screening of clinical samples.

The utility model is designed for the rapid (up to 20 minutes) detection of at least 10 types of analytes in environmental samples (water, air, soil, etc.) and clinical specimens. The immunochromatographic test strip for multiplex analysis includes a polymer substrate with an applied immunochromatographic membrane and a capture pad. The test and control zones of the test strip are designed as two rows of transverse microstrips occupying no more than half the width of the test strip. The test strip includes a marker for coordinating the cutting of test strips. The technical result consists in reducing the time for recording the luminescent signal from test strips in an automated system. 3 c.p. phyl., 3 fig., 2 tables.

The application discloses an anti-LMBV single-chain antibody and application thereof in preparation of a quantum dot fluorescent immunochromatography test strip, and belongs to the technical field of biological detection. The single-chain antibody comprises Ab-K1 and Ab-K2, the amino acid sequence of the Ab-K1 is shown as SEQ ID NO. 11, and the amino acid sequence of the Ab-K2 is shown as SEQ ID NO. 12. The single-chain antibody used in the application can accurately recognize and combine with iridovirus in a sample to be detected, so that the accuracy of detection is ensured. Meanwhile, the antibody labeled with fluorescent quantum dots has good fluorescent characteristics, so that the fluorescent signal is easy to observe and detect, and the specific surface area is relatively large, so that the antibody can be combined with more virus molecules, so that the sensitivity of detection is improved, the accuracy and sensitivity of detection are improved, the operation steps are simplified, and powerful technical support is provided for detection and prevention and control of iridovirus.

The invention provides a fluorescence immunochromatography test strip rapid reading and analyzing method based on deep learning and application. The method comprises the following steps: acquiring fluorescence spectrum data of a to-be-detected sample on a fluorescence immunochromatography test strip; constructing a signal preprocessing model; constructing a signal feature extraction model; constructing a time sequence prediction model; constructing a detection result output model; the method comprises the following steps: inputting a collected reaction phase fluorescence intensity signal to a signal preprocessing model for time domain distinguishing labeling, performing signal feature vector extraction by a signal feature extraction model, performing reaction complete feature vector prediction by a time sequence prediction model, and finally obtaining a detection result of a to-be-detected object of a to-be-detected sample through a detection result output model. Through a deep learning method, a to-be-detected object can be predicted and detected according to a reaction signal without waiting for complete reaction on the fluorescence immunochromatography test strip, and the requirement for rapid detection is met.

The present invention discloses a method for preparing water-soluble quantum dots. The preparation method uses a surfactant solution to coat oil-soluble quantum dots, retaining the fluorescence of the oil-soluble quantum dots, and uses water-soluble polymer ligands (polyvinyl alcohol and polyvinyl pyrrolidone) to complete functional modification, thereby solving the problems of easy quenching of quantum dot fluorescence, inability of nanoparticles to stably agglomerate, and prone to nonspecific adsorption, resulting in low detection sensitivity. When the water-soluble quantum dots prepared by the preparation method of the present invention are used for biomarker detection, they can be directly dissolved in water, and the fluorescent group is stable, can stably agglomerate and specifically adsorb biological targets; therefore, the immunochromatographic test strips prepared using the water-soluble quantum dots can simply and stably adsorb drug antibodies, thereby improving detection sensitivity, with the detection sensitivity for morphine reaching 0.1 ng / mL.

The invention belongs to the technical field of immunodetection, and particularly relates to an SERS (Surface Enhanced Raman Scattering) lateral flow immunochromatographic test strip and a preparation method thereof.The SERS signal molecule 4-NTP is encapsulated between a silver core and a gold shell by preparing Ag4-NTP-coated Au NPs nanoparticles and an SERS layered magnetic colorimetric nano-label, and a Raman signal is enhanced; semi-quantitative analysis is carried out by observing the color of the T line after competitive immunoreaction, or quantitative analysis is realized by measuring the SERS signal intensity of the T line; under the condition that the T line of the test strip can perform multi-color display, compared with the traditional test strip detection method, the Raman signal detection involved in the invention has more direct and sensitive display on the actual detection result.

This immunochromatographic test piece is used for detecting a test substance in a sample containing blood cells by immunochromatography and comprises: a matrix for developing a development liquid; a blood cell removal pad that is layered on the matrix and includes an addition section for the sample; and a waterproof layer layered between the matrix and the blood cell removal pad. The blood cell removal pad has a protruding section protruding further onto the matrix in the development direction of the development liquid than the waterproof layer.

The application provides a quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids and an application thereof, and belongs to the technical field of veterinary biological diagnosis products. The quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids comprises a base plate, on the base plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are sequentially connected in a head-to-tail mode, from the combination pad to the water absorption pad, the nitrocellulose membrane is sequentially provided with an antibody detection line, a nucleic acid detection line and a quality control line, the antibody detection line is coated with a goat anti-pig IgG-Fc antibody, the nucleic acid detection line is coated with streptavidin, and the quality control line is coated with a rabbit anti-sheep IgG antibody; the combination pad is coated with a quantum dot microsphere labeled recombinant protein P22 and a quantum dot microsphere labeled sheep anti-digoxin antibody. The test paper can rapidly, highly specifically and highly sensitively detect ASFV antibodies and nucleic acids.

The invention belongs to the technical field of food safety monitoring and immunoassay detection, and particularly relates to a colloidal gold immunochromatography test strip, a preparation method and application thereof, the colloidal gold immunochromatography test strip comprises a bottom plate, a nitrocellulose membrane, absorbent paper, a conjugate pad and a sample pad; a mycobacterium paratuberculosis monoclonal antibody marked by colloidal gold is adsorbed on the combination pad; a detection line coated with the mycobacterium paratuberculosis monoclonal antibody and a quality control line formed by goat anti-mouse-IgG are arranged on the nitrocellulose membrane; and the bottom plate is a polyethylene chloride bottom plate, so that the MAP in the fresh milk can be rapidly and accurately detected.