Magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and preparation method thereof

Abstract

The invention relates to a magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and a preparation method thereof. The test strip is assembled by pasting a coated film, magnetic particles combined with an AFP antibody, a sample pad and a water-absorbing pad which are mutually staggered by 2 millimeters in turn on a bottom plate and then covering an upper layer with a transparent plastic sealing film, wherein the coated film is precoated with an AFP-antibody detection line and a quality control line. As the test strip introduces a magnetic immunochromatography technique and a biotin-avidin system into the quantitative detection of AFP in blood, the test strip has the advantages of greatly increasing detection sensitivity, providing accurate quantitative results, reducing the labor intensity of operators and ensuring that clinicians can quickly diagnose patients.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and a preparation method thereof. Background technique [0002] Alpha-fetoprotein (Alpha-fetoprotein, AFP) is a glycoprotein with a relative molecular weight of 65,000-72,000, which is composed of 18 kinds of amino acids and carbohydrates account for about 4%, which is produced by the human liver and yolk sac (fetus). . The content of alpha-fetoprotein varies greatly in different stages of the human life cycle. It is very high in fetal blood. It is synthesized by liver and yolk sac in the early stage of fetal development, and a small amount is synthesized by fetal vitellin cells and fetal gastrointestinal tract. - Synthesis begins at 10 weeks, reaches peak at 12-15 weeks, and a small amount passes through the placenta to maternal blood to increase AFP. After the fetus is born, alph...

AI Technical Summary

Problems solved by technology

[0003] The current diagnostic techniques for AFP in blood mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence (CLIA), time-resolved fluorescence, colloidal gold immunochromatography and other methods, but these methods have their own Features and disadvantages: radioimmunoassay is a commonly used method in clinical practice. Its advantages are relatively accurate results and wide linear range. The disadvantages are cumbersome and time-consuming operations, and radioactive markers will cause harm to the operator and cause environmental pollution. It has been gradually replaced by other methods

ELISA is similar to chemiluminescence and time-resolved fluorescence method in principle, but there is a difference in sensitivity due to the difference in the final detection system. At present, automation, large-scale, and quantitative detection have been realized, but there are large differences in results between methods, and the linear range is relatively small. At the same time, automated testing is currently monopolized by large foreign manufacturers, and the equipment is expensive, and it is not suitable for single-person and small-batch testing, which greatly limits their application in primary hospitals and clinics.

In recent years, colloidal gold immunochromatography has appeared to detect AFP, which is fast, convenient, and single-serve operation, but the disadvantage is that it can only achieve semi-quantitative, can only indicate a general range, and cannot achieve accurate quantification, and abnormal samples need to use other methods Double-check testing will virtually increase the cost of medical treatment for the testees, and it is difficult to promote in the market, and it is difficult to use it on a large scale

Images