Preparation method of magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood
A magnetic immunochromatography, alpha-fetoprotein technology, applied in the field of medical testing, can solve the problems of narrow linear range, operator harm, difficult to use in a large area, etc., and achieve the effects of simplified preparation process and easy operation.
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Embodiment 1
[0035] Preparation method of magnetic immunochromatography test strip and test box for quantitative detection of alpha-fetoprotein in blood
[0036] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:
[0037] A. Antibody preparation: commercial AFP paired antibody (Hytest company, cat: 4F16) was selected, dialyzed against 20 mM PBS at pH 7.2 (pH7.0-7.6 is applicable) overnight at 4°C for later use.
[0038] B. Preparation of coating film:
[0039] Preparation of coating buffer: 0.02M phosphate buffer (PB) with pH 7.2 was used as coating buffer, sterilized by 0.22 μm microporous membrane filter and stored at 4°C for later use, valid for two weeks.
[0040] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA, pH 7.2 (pH 7.0-7.6 is applicable), 0.22μm microporous membrane filter sterilization, and store at 4°C for later use , valid for one week.
[0041] Preparation of coating...
Embodiment 2
[0070] Except in the step of the preparation of streptavidinized magnetic particles: streptavidin makes the ratio of streptavidin and magnetic particles 1:2, other steps are the same as in Example 1,
Embodiment 3
[0072] Except for the step of preparing streptavidinized magnetic particles: streptavidin makes the ratio of streptavidin to magnetic particles 1:10, other steps are the same as in Example 1.
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