148 results about "Linear range" patented technology

The invention relates to the technical field of vacuum measurement, in particular to a device for expanding the linear range of mass spectrometry measurement, which comprises a gas transmission pipeline, an electromagnetic valve, a small hole and a mass spectrometer, and is characterized in that the front end of the gas transmission pipeline is used for allowing a sample to be measured to enter, and the rear end of the gas transmission pipeline is connected with the mass spectrometer; the middle part of the gas transmission pipeline is divided into three channels, namely a first gas transmission pipeline, a second gas transmission pipeline and a third gas transmission pipeline; the first electromagnetic valve and the first small hole are sequentially arranged on the first gas transmission pipeline, the second electromagnetic valve and the second small hole are sequentially arranged on the second gas transmission pipeline, and the third electromagnetic valve and the third small hole are sequentially arranged on the third gas transmission pipeline. According to the method, manual dilution or segmented sample injection is not needed, a wide concentration range can be covered by single analysis, more than 60% of time cost is saved, and measurement in a dynamic range of 106 is realized.

The invention discloses a flexible piezoresistive sensor and a preparation method and application thereof, the flexible piezoresistive sensor comprises an upper layer flexible conductive member, the upper layer flexible conductive member comprises an upper layer flexible substrate and an upper layer conductive layer, one surface of the upper layer flexible substrate is provided with a microstructure formed by sand paper reverse molding, and the upper layer conductive layer is embedded in the surface of the microstructure; the lower-layer flexible conductive member comprises a lower-layer flexible substrate, a lower-layer conductive layer and an elastic conductive layer; the lower-layer flexible substrate is provided with an arc-shaped stripe array microstructure; the elastic conductive layer includes a polymer matrix and a conductive filler dispersed therein. Through the double-gradient design of the gradient conductivity and the gradient structure, the core contradiction that a traditional flexible piezoresistive sensor is difficult to consider high sensitivity and wide linear range at the same time is successfully solved by utilizing the deep coupling and synergistic effect of the conductivity gradient and the structure gradient, and synchronous improvement of the sensitivity and the linear range is realized.

The invention discloses a flexible three-dimensional force tactile sensor and a preparation method thereof, and belongs to the technical field of flexible tactile sensors. The flexible three-dimensional force tactile sensor solves the problem that when an existing flexible three-dimensional force tactile sensor senses tangential force, the wide linear range and the high sensitivity contradict each other, and the requirements that the sensitivity and the linear range can be flexibly adjusted and customized are met. According to the invention, the sensitive layer with transverse gradient change of modulus is constructed in a manner of sleeving and splicing materials with different moduli, so that the sensitivity of the sensitive layer is changed transversely, and the sensitive layer is used for regulating and controlling the change of the sensitivity and the linear range when the sensor detects tangential force; the non-uniformity of the surface stress distribution of the sensitive layer under the action of the tangential load is utilized by utilizing the sensitivity of the non-uniform transverse distribution of the sensitive layer, so that the tangential force sensing performance of the device is further improved, the method is also effective under the normal load, and the three-dimensional force sensor prepared by the method has high sensitivity and wide linear range, and can be applied to the field of sensor devices. The method is widely applied to the aspects of three-dimensional force measurement, direction control and the like.

The invention provides a method for specifically detecting AFP based on a photoinduced electron transfer effect between blood orange RCDots and an AFP-aptamer, and the preparation and AFP detection principles of a near-infrared PET fluorescent probe for AFP detection are as follows: the RCDots with rich carboxyl groups on the surface can strongly adsorb ssDNA to the surface of the RCDots through pi-pi stacking interaction; and a formed RCDots / AFP-aptamer compound is subjected to a photo-induced electron transfer effect, so that the fluorescence of the RCDots is remarkably quenched. However, the affinity between the AFP and the AFP-aptamer is stronger than pi-pi stacking interaction, so that ssDNA is separated from the surface of the RCDots, the light-induced electron transfer effect disappears, the fluorescence of the RCDots is recovered, and the fluorescence intensity is changed along with the change of the concentration of the AFP, so that the concentration of the AFP is detected with one kind of specificity. The detection method is simple, has the characteristics of wide detection linear range, low detection limit, good specificity and the like, and can be widely applied to clinical detection of liver cancer markers.

The invention provides a wide-spectrum dual-photoelectrode-based photoelectrochemical sensor and a preparation method and application thereof, and belongs to the technical field of photoelectrochemical sensors. According to the photoelectrochemical sensor provided by the invention, a BCN / ITO electrode is used as a photoanode, a CuInS2 / ITO electrode is used as a photocathode, BCN is Bi2S3 / C3N4, and ITO is indium tin oxide. The sensor is used for detecting enrofloxacin and ciprofloxacin pollution. According to the present invention, the surface is modified with the aptamer having the specific recognition function, the specific binding effect of the aptamer with the enrofloxacin and the ciprofloxacin is utilized to form the complex of the aptamer and the target object, and the influence of the caused steric hindrance effect on the photoelectric signal and the dual-channel strategy are utilized to achieve the simultaneous detection of the residual marker; the linear ranges for detecting enrofloxacin and ciprofloxacin are 0.1 nM to 1 mu M and 50 pM to 0.5 mu M respectively, the detection limits are 24 pM and 15.5 pM respectively, and the method is successfully applied to simultaneous detection of residual markers in water. The method has the advantages of greenness, environmental protection, wide detection range, high sensitivity, good selectivity and high stability.

The invention relates to a lanthanide oxide electrochemiluminescence aptamer sensor with dual signal amplification for detecting acetamiprid as well as a preparation method and application of the lanthanide oxide electrochemiluminescence aptamer sensor. According to the sensor, terbium-doped europium oxide (Eu2O3: Tb < 3 + >) modified by gold nanoparticles (Au NPs) is used as a luminous body, and the electrochemical luminescence performance can be remarkably improved through multiple mechanisms: on one hand, the Au NPs can effectively promote generation of more sulfate free radicals; on the other hand, the surface defect passivation effect and the efficient synergistic effect of energy transfer jointly achieve effective amplification of signals. In addition, the wool ball-shaped Ag NPs is used as a plasma resonance signal amplification source, so that the ECL intensity of the sensor is further enhanced. The prepared electrochemical luminescence sensor with the dual signal amplification effect is used for detecting acetamiprid (ACE), has a wide linear range and a low detection limit, and has a wide application prospect in detection of acetamiprid residues in a water environment.

The invention belongs to the technical field of biological analysis and detection, and particularly relates to a photoelectrochemical-colorimetric dual-mode biosensor and application thereof in miRNA detection. According to the biosensor prepared by the invention, the bifunctional CuO-Cu2O nanoflower and the three-dimensional network g-C3N4 / AuNPs electrode are integrated, and an enzyme-assisted strand displacement signal amplification mechanism is combined, so that dual-mode collaborative detection of a target miRNA-21 is realized: in a photoelectrochemical mode, the detection limit reaches 0.35 fM (the linear range is 1 fM to 10 fM), and response is performed based on photocurrent polarity reversal; the colorimetric mode is as follows: the detection limit is 3.9 fM (the linear range is 10 fM to 10 nM), and TMB (Tetramethylbenzidine) color development visualization analysis is carried out. Single-mode interference is effectively eliminated through double-signal independent output and cross validation, and the method is suitable for high-reliability detection of trace miRNA (such as a breast cancer marker miRNA-21) in complex samples such as serum. According to the invention, the defects of insufficient sensitivity (pM level), poor anti-interference performance and high platform complexity in the prior art are overcome, and an efficient tool is provided for early diagnosis of cancers.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The invention discloses an underwater CSK-OFDM modulation and detection method based on LED linear constraint, and the method comprises the steps: enabling a transmitter to map a data bit stream of a constellation diagram into a complex symbol stream, converting the complex symbol stream into a real number time domain sequence through an ACO-OFDM module, carrying out the LED amplitude limiting of the time domain sequence, and obtaining a time domain clipping sequence; transmitting the time domain clipping sequence in a UOWC channel through a corresponding LED, introducing symbol timing deviation and amplitude limiting noise into a frequency domain by a receiver, and processing the time domain clipping sequence by using an ACO-OFDM module again to obtain a received signal; under the maximum expectation framework, the probability from each alternative constellation diagram is calculated, symbol timing deviation and additive white Gaussian noise variance are estimated, a globally optimal solution is obtained, and a constellation diagram recognition result is output through maximum likelihood demodulation. According to the method, the linear range constraint of the LED and the complex environment under the UOWC scene are comprehensively considered.

The invention relates to a protective circuit (1) for a device or power circuit breaker comprising, • an active controllable component (B1) which can be controlled in a linear range, • at least one measuring device (S P , S U , S i , S Uin , S Uout ) to determine a power output that is implemented in the actively controllable component (B1), • a processing unit (uC), • where an energy equivalent can be determined based on the specified power and a time between two measurements, • where time-separated energy equivalents are combined by the processing unit (uC), • where at least two time-related limit values ​​(G t1 , G t2 , ... G tn ) are planned, • where, upon reaching and / or exceeding a limit value (G t1 , G t2 , ... G tn) by an energy equivalent or the combined energy equivalents in a limit value (G t1 , G t2 , ... G tn ) assigned time the active controllable component (B1) is controlled by the processing unit (uC) in such a way that the current flow through. Furthermore, the invention relates to a method.

This invention discloses a method and application for constructing a dual-mode sensor for detecting diphenyl phosphate using smartphone-assisted surface molecular imprinting, belonging to the fields of biochemical analysis, functional nanomaterials, and life science technology. In the synthesis process, the solubility, selectivity, and photoinitiation efficiency of the target analyte during polymerization are optimized, and molecularly imprinted nanozymes (MIPs) for diphenyl phosphate are rapidly prepared using molecular imprinting technology. In the detection process, the peroxidase-like activity of Fe,N-CDs catalyzes the colorimetric reaction between H₂O₂ and 3,3,5,5-tetramethylbenzidine (TMB), and the internal filtering effect of oxTMB suppresses the fluorescence signal. Combined with a smartphone, dual-signal cross-validation is achieved, constructing a colorimetric-fluorescence sensor for detecting diphenyl phosphate. This sensor exhibits specific recognition selectivity and binding affinity for diphenyl phosphate, is simple to operate, and low in cost. It can achieve sensitive detection of diphenyl phosphate over a wide linear range, with a detection limit as low as 16 pg / mL, making it suitable for on-site analysis of trace DPH in complex aquatic environments.

The invention provides a method for detecting the content of zinc in a lithium-zinc binary alloy strip based on ICP-OES, and belongs to the technical field of metal material component analysis. According to the method, the lithium matrix is separated by adopting a nitric acid-perchloric acid digestion system, and the stock solution is diluted by 500 times, so that the concentration of the lithium matrix can be efficiently reduced, meanwhile, the signal inhibition effect is effectively eliminated by combining lithium salt matching, and the detection precision is greatly improved. According to the method, Zn 213.856 nm analysis spectral lines are carefully selected, RF power is optimized, spectral interference of other elements can be avoided, the linear range can be expanded, and the method is suitable for rapid determination of the zinc content of 1%-20%. According to the method, the detection limit can reach 0.01%, the relative standard deviation is smaller than or equal to 1.0%, the recovery rate is 98.5%-101.2%, and high-precision detection is achieved.

This invention provides a method for fabricating a tactile sensing unit capable of independently adjusting sensitivity and linear range, belonging to the fields of flexible wearables, tactile sensing, and human-computer interaction. The method includes the following steps: S1, determining the structure of the tactile sensing unit; S2, establishing a correlation model between the structural parameters of the bi-phase constrained elastomer and the range and sensitivity of the sensing unit; S3, based on the target linear range and target sensitivity, obtaining the structural parameters of the high-stiffness outer ring and the negative Poisson's ratio inner ring through the correlation model; S4, fabricating the bi-phase constrained elastomer according to the structural parameters of the high-stiffness outer ring and the negative Poisson's ratio inner ring, and assembling and packaging it. This invention achieves synergistic optimization of wide linear range and high sensitivity through the bi-phase constrained elastomer structure, and supports reverse design with calculable parameters, manufacturable structure, and integrable functions based on target performance indicators.

This invention relates to the field of pressure (0~200 kPa) detection, and more particularly to MEMS integrated pressure sensor devices or monolithically integrated multi-frequency capacitive micromechanical ultrasonic transducers (CMUTs) for wide-range pressure measurement. The monolithically integrated multi-frequency CMUTs integrate a first CMUT, a second CMUT, and a third CMUT on a single chip. By adjusting the diaphragm thickness, cavity height, diaphragm radius of the first, second, and third CMUT units, as well as the pressure value within the cavity of the second CMUT unit, the first and second CMUT units operate within a linear range of small deflection deformation, and the third CMUT unit operates within a linear range of large deflection deformation. Furthermore, the operating ranges of the first, second, and third CMUT units constitute a continuous and complete measurement interval, with resonant frequencies that do not overlap, thus realizing an integrated pressure sensor device usable for measuring the entire pressure range of 0~200 kPa.

This invention relates to a method for detecting defoamer 204, belonging to the field of bioproduct quality testing. This invention employs HPLC-ELSD to detect defoamer 204, providing a solution for determining the content of defoamer 204 in bioproducts by optimizing sample pretreatment conditions, HPLC parameters (mobile phase selection, elution gradient), and ELSD parameters (carrier gas flow rate, drift tube temperature, detector gain). Furthermore, this invention exhibits a wide linear range (R04 at 2.5~50 μg / mL) when used for defoamer 204 detection. 2 With advantages such as >0.99, high accuracy (recovery rate of 91.4~98.5%), high sensitivity (limit of detection of 1.0 μg / mL, limit of quantitation of 2.5 μg / mL), good specificity, and simple operation, it can accurately detect the presence and content of defoamer 204 in biological products, filling a gap in the field and having great significance for the quality monitoring and control of biological products.

The invention discloses a method for detecting the content of methanol in histidine, which adopts headspace sampling-gas chromatography and comprises the following steps: preparing a reference solution; preparing a test solution; performing chromatographic analysis; and measuring and calculating. According to the gas chromatography determination method for the methanol content in the histidine, high-sensitivity and high-accuracy detection of methanol residues in the histidine is realized by adopting a headspace sampling mode and combining with optimized chromatographic conditions. The method is high in specificity, wide in linear range, good in repeatability, excellent in durability and suitable for conventional detection of methanol residues in drug quality control.

The invention relates to a method for determining the total amount of impurity elements in a natural high-purity quartz mineral, which comprises the following steps of: preparing a laser sheet of the quartz mineral, observing and photographing by using a polarizing microscope, designing an analysis test point on a picture, and carrying out point-by-point analysis and determination on a laser ablation and inductively coupled plasma spectrum according to the design point. Accurate determination of impurity elements of quartz minerals in a natural high-purity quartz raw material is realized, comprehensive analysis and evaluation are carried out, and grade characteristics of the quartz minerals in the high-purity quartz raw material are further determined. The method is suitable for analysis and determination of impurity elements in natural quartz minerals, has the advantages of low detection limit, high sensitivity, wide linear range and the like, can be applied to effective and rapid evaluation, quality monitoring and the like of high-purity quartz raw materials, and provides quartz mineral raw materials with stable grades for the high-purity quartz sand industry.

The invention discloses an analog signal range judgment and switching system with calibration and self-diagnosis functions, which relates to the technical field of analog signal monitoring and comprises an analog signal acquisition module, an analog signal range analysis module, an analog signal range switching module and a database, the method comprises the following steps: firstly, analyzing the influence of different environment temperature intervals on the optimal linear interval of the ADC to obtain the optimal linear interval of the ADC in the different environment temperature intervals, determining the optimal linear interval of the ADC according to the current environment temperature, and judging whether an analog signal falls into the optimal linear interval of the ADC and whether the current range gear loses efficacy; and then the target range gear is obtained by analyzing the influence among the range gears under different gear working conditions, and the current gear is switched to the target range gear, so that the effectiveness of range analysis and the precision of ADC sampling are guaranteed, and the rationality of range switching is also guaranteed.

The invention relates to a fluorescence and colorimetric dual-mode biosensing method for detecting caffeine and application thereof, the method causes CRISPR / Cas13a to regulate and control Fe-MOFs to fall off from a signal probe based on to-be-detected liquids with different concentration gradients, the fallen Fe-MOFs collapses under the action of sodium pyrophosphate to release 2-aminoterephthalic acid and ferric ions, and the 2-aminoterephthalic acid and ferric ions are subjected to fluorescence and colorimetric dual-mode biosensing method is used for detecting caffeine. 2-aminoterephthalic acid is converted into a fluorescence signal, meanwhile, Prussian blue generated by reaction of ferric ions and potassium ferrocyanide is converted into a colorimetric signal, and a fluorescence and colorimetric dual-mode biosensing method is constructed; according to the sensor, interference factors can be effectively eliminated by outputting two detection signals and performing cross validation; the advantages of high sensitivity and wide linear range of a fluorescence method and the advantages of simple operation and visual result of a colorimetric method are combined, so that a target object can be accurately recognized, the lower detection limit can be reduced, and the accuracy of a quantitative result can be ensured; according to the sensing method, visual observation can be achieved, precise instruments are not needed, and good application value is achieved.

The invention discloses a preparation method of an Fc (at) COFs / rGO / GCE electrode, an electrochemical sensor and application of the electrochemical sensor in detection of fenitrothion. The sensor is prepared by modifying a glassy carbon electrode with a ferrocene functionalized covalent organic framework / reduced graphene oxide (Fc (at) COFs / rGO) composite material. The Fc (at) COFs / rGO composite material is prepared by the following steps: firstly synthesizing COFs from 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-dimethoxybenzene-1, 4-dicarboxaldehyde, and then respectively adding Fc and rGO to enable the Fc and the rGO to be combined on the surface of the COFs to form the Fc (at) COFs / rGO composite material. The electrochemical sensor can provide a stable internal standard output signal through Fc, the signal and a response signal of fenitrothion are synchronously output in the detection process and do not interfere with each other, so that a stable ratio sensor is formed to sensitively and accurately detect fenitrothion, the linear range of the sensor is 1-50000 ng / mL, and the detection limit is 0.33 ng / mL; the sensor has the advantages of high detection speed, high sensitivity, high precision and the like.

A magnetoresistive sensor with harmonically broadened linear range comprises a substrate (100) and a multiple push-pull magnetoresistive sensing bridge arms. The push arms (91) of the sensing bridge comprise N types of push magnetoresistive sensing units (94). The pull arms (92) comprise N types of pull magnetoresistive sensing units. Key characteristic parameters of each magnetoresistive sensing unit comprise R0i, MRi, Hsi, ±αpi and ai. The Key characteristic parameters of the push magnetoresistive sensing units (94) and corresponding pull magnetoresistive sensing units are different from the key characteristic parameters of standard magnetoresistive sensing unit of a standard push-pull linear magnetoresistive sensor. There is at least one set of key characteristic parameters [(R0j, MRj, Hsj, ±αpj), aj]. Linear parts and nonlinear harmonic parts of R-H characteristic parameters that characterize the push magnetoresistive sensing units and the pull magnetoresistive sensing units are superimposed, so that the linear range of the multiple push-push magnetoresistive sensing bridge is greater than the linear range of the standard push-pull linear magnetoresistive sensor.

The invention relates to the technical field of enzyme activity detection, in particular to a standardized Glu-C incision enzyme activity spectrum detection method. The method provided by the invention realizes rapid, accurate and low-cost enzyme activity detection by using a specific substrate Z-Phe-Leu-Gl-4-pNA and optimized reaction conditions, and specifically comprises the following steps: diluting a Glu-C endonuclease sample, mixing the diluted Glu-C endonuclease sample with a substrate solution, putting the mixture into a preheated Tris-HCl buffer solution, monitoring absorbance change at the wavelength of 405nm, and calculating the enzyme activity through a specific formula. A standardized Glu-C incision enzyme activity detection system is established, the detection time is short, the cost is low, the detection method has a wide linear range, a low detection limit and high precision, and a technical basis is provided for establishing an industrial unified standard.

The present invention belongs to the field of analytical chemistry and biosensor technology, and relates to a preparation method and application of a photoelectrochemical-surface enhanced Raman spectroscopy sensor. The sensor utilizes the excellent light absorption ability and Raman scattering characteristics of Zr-MOF, and can simultaneously generate obvious PEC and SERS signals under low-power 532nm Raman excitation light. And compared with porphyrin molecules, the Raman signal stability of Zr-MOF is better. Prepare AuNDs@AgNPs as a dual-functional enhanced substrate to enhance the photoelectric and Raman signals of Zr-MOF; use aptamers to connect the dual-functional nanoprobe Zr-MOF and AuNDs@AgNPs to construct a photoelectrochemical-surface enhanced Raman spectroscopy sensor, and use the dual signals to collect simultaneously on the same electrode and refer to each other, thereby achieving high sensitivity and high precision detection of penicillin, with a detection linear range of 1pg mL ‑1 ~100ng mL ‑1 .

The present invention belongs to the technical field of biomedical detection and in-vitro diagnosis. Disclosed are a colorimetric-fluorescent dual-mode nanoprobe and microfluidic chip for detecting cMyBP-C. In the present invention, a colorimetric-fluorescent dual-mode nanoprobe, which comprises UCNPs as a core and MOF as a shell and is loaded with horseradish peroxidase and a labeled antibody, is prepared by means of a "layer-by-layer self-assembly" technique; and then a microfluidic chip is prepared on the basis of the nanoprobe. The microfluidic chip realizes dual-mode detection of colorimetric and fluorescent signals of cMyBP-C, thereby solving technical problems such as limited sensitivity, susceptibility to background interference, limited linear range, photobleaching and signal instability in single-signal detection.

The embodiment of the invention provides a high-gain broadband low-noise amplifier with a high linear range. The circuit is applied to the radio frequency integrated circuit technology and comprises a first-stage amplification circuit, a second-stage amplification circuit and a matching network, and the first-stage amplification circuit and the second-stage amplification circuit are connected through the matching network; the first-stage amplification circuit adopts a cascode structure formed by a single tube and is used for performing first-stage amplification on an input signal; the second-stage amplification circuit adopts a cascode structure formed by composite tubes and is used for performing second-stage amplification on the input signal; and the matching network is used for receiving an output signal of the first-stage amplifying circuit and inputting the output signal of the first-stage amplifying circuit into the second-stage amplifying circuit. The design meets the requirements of high gain, low noise and high linearity.

A magnetoresistive sensor with harmonically broadened linear range comprises a substrate (100) and a multiple push-pull magnetoresistive sensing bridge arms. The push arms (91) of the sensing bridge comprise N types of push magnetoresistive sensing units (94). The pull arms (92) comprise N types of pull magnetoresistive sensing units. Key characteristic parameters of each magnetoresistive sensing unit comprise R0i, MRi, Hsi, ±αpi and ai. The Key characteristic parameters of the push magnetoresistive sensing units (94) and corresponding pull magnetoresistive sensing units are different from the key characteristic parameters of standard magnetoresistive sensing unit of a standard push-pull linear magnetoresistive sensor. There is at least one set of key characteristic parameters [(R0j, MRj, Hsj, ±αpj), aj]. Linear parts and nonlinear harmonic parts of R-H characteristic parameters that characterize the push magnetoresistive sensing units and the pull magnetoresistive sensing units are superimposed, so that the linear range of the multiple push-push magnetoresistive sensing bridge is greater than the linear range of the standard push-pull linear magnetoresistive sensor.

One aspect provides a dual-scale counter circuit that includes a counter logic unit for storing the current counter value, a range determination logic unit for determining the operating range of the dual-scale counter circuit based on the current counter value and a predetermined threshold, and a counter increment logic unit. The counter increment logic unit is used to linearly increment the current counter value for an increment event in response to the dual-scale counter circuit operating in a linear range, and to probabilistically increment the current counter value for the increment event in response to the dual-scale counter circuit operating in a probabilistic range.The dual-scale counter circuit also includes a linear feedback shift register for generating a random binary bit sequence, on the basis of which the counter increment logic unit is to determine whether the current counter value should be increased for the increment event when the dual-scale counter circuit is operating in the probabilistic range.

The invention relates to the technical field of chemical analysis, and discloses a photoelectrochemical aptamer sensor as well as a preparation method and application thereof. The photoelectrochemical aptamer sensor comprises a conductive substrate, and a Bi / Bi2MoO6 / phosphorus-doped g-C3N4 heterojunction composite material, an aptamer and bovine serum albumin which are sequentially modified on the surface of the conductive substrate. According to the photoelectrochemical aptamer sensor provided by the invention, photocurrent response is remarkably improved through the Bi / Bi2MoO6 / phosphorus-doped g-C3N4 heterojunction composite material, and high-sensitivity, wide-linear-range, quick-response and excellent-selectivity detection of tetracycline is realized by combining high-specificity recognition of the aptamer and an anti-interference blocking effect of bovine serum albumin. Compared with a traditional method, the photoelectrochemical aptamer sensor has the advantages of being simple in equipment, low in cost, free of external voltage, good in stability, good in anti-interference performance and the like.

This invention provides a method for preparing and applying a LIG molecularly imprinted electrochemical sensor based on the synergistic enhancement of hydrophobic comonomers N-MCz-PABA and AuNPs. Using LIG as the substrate electrode, the invention copolymerizes the hydrophobic monomer N-MCz, the electropolymerizable monomer PABA, and AuNPs precursors onto the electrode surface via a one-step electropolymerization method, forming an N-MCz-PABA@Au molecularly imprinted composite film with both high-affinity recognition sites and high conductivity. This sensor utilizes the enhanced hydrophobic interaction provided by N-MCz to dominate the specific recognition of cortisol, and leverages the in-situ embedded AuNPs to improve interfacial electronic conductivity, combined with the exogenous redox probe K3[Fe(CN)6] to achieve signal transduction. Its detection linear range for cortisol is 0.01–100 μM, with a detection limit of 4.6 nM. This invention features a simple preparation process, low cost, high sensitivity, and good selectivity, providing an effective platform for the immediate and rapid detection of hydrophobic small molecules in complex biological matrices such as sweat.

The invention provides a photoelectrochemical sensing detection module and system and application thereof. The detection module comprises an electrolytic tank with an upper opening and a lower opening, a working electrode, a counter electrode, a light source and a packaging structure, the working electrode and the electrolytic tank are hermetically connected to form a cavity; at least part of the counter electrode is arranged in the cavity; the light source provides exciting light for the surface of the working electrode; the packaging structure fixedly connects all the components into an integrated structure, and a light path between the light source and the working electrode is fixed. The module is compact in structure, easy and convenient to operate and portable, light path deviation is avoided through fixed packaging of the light source, the cavity stability is guaranteed through the sealing ring, and the module is low in signal background noise, high in signal-to-noise ratio and good in result reproducibility in the detection process; the photoelectrochemical sensing detection system is matched with the specific molecularly imprinted material layer, high-performance detection of bisphenol A is achieved, the linear range reaches 0.1 microgram L <-1 > to 500 microgram L <-1 >, and the detection limit is as low as 0.1 microgram L <-1 >.