236 results about "Linear range" patented technology

The invention provides an integrated electrochemical biosensor capable of detecting double miRNA with high sensitivity, and the integrated electrochemical biosensor can simultaneously detect double miRNA markers with high sensitivity through self-assembly of a Y-shaped capture probe, preparation of an Au / TiO2-FTO array electrode, catalytic hairpin amplification (CHA) and construction of a micro-droplet chip electrochemical biosensor. The advantages of equalization of double-target miRNA capture probe sites, excellent electrode performance, reliable signal amplification, low sample consumption and the like are integrated, the linear range of miRNA21 and miRNA155 detection is 10 fM to 100 nM, and the detection limits are 5.00 fM and 5.17 fM respectively. By detecting the miRNA21 and miRNA155 double markers, the accuracy of breast cancer diagnosis is up to 98%, and the recognition sequences at the two ends of the Y stent probe are modified, so that the sensor can be used for diagnosing other miRNA marker related diseases. The integrated micro-droplet chip electrochemical biosensor constructed by the invention has a remarkable clinical diagnosis value and a prominent practical application prospect.

The invention relates to the technical field of vacuum measurement, in particular to a device for expanding the linear range of mass spectrometry measurement, which comprises a gas transmission pipeline, an electromagnetic valve, a small hole and a mass spectrometer, and is characterized in that the front end of the gas transmission pipeline is used for allowing a sample to be measured to enter, and the rear end of the gas transmission pipeline is connected with the mass spectrometer; the middle part of the gas transmission pipeline is divided into three channels, namely a first gas transmission pipeline, a second gas transmission pipeline and a third gas transmission pipeline; the first electromagnetic valve and the first small hole are sequentially arranged on the first gas transmission pipeline, the second electromagnetic valve and the second small hole are sequentially arranged on the second gas transmission pipeline, and the third electromagnetic valve and the third small hole are sequentially arranged on the third gas transmission pipeline. According to the method, manual dilution or segmented sample injection is not needed, a wide concentration range can be covered by single analysis, more than 60% of time cost is saved, and measurement in a dynamic range of 106 is realized.

The invention discloses a flexible piezoresistive sensor and a preparation method and application thereof, the flexible piezoresistive sensor comprises an upper layer flexible conductive member, the upper layer flexible conductive member comprises an upper layer flexible substrate and an upper layer conductive layer, one surface of the upper layer flexible substrate is provided with a microstructure formed by sand paper reverse molding, and the upper layer conductive layer is embedded in the surface of the microstructure; the lower-layer flexible conductive member comprises a lower-layer flexible substrate, a lower-layer conductive layer and an elastic conductive layer; the lower-layer flexible substrate is provided with an arc-shaped stripe array microstructure; the elastic conductive layer includes a polymer matrix and a conductive filler dispersed therein. Through the double-gradient design of the gradient conductivity and the gradient structure, the core contradiction that a traditional flexible piezoresistive sensor is difficult to consider high sensitivity and wide linear range at the same time is successfully solved by utilizing the deep coupling and synergistic effect of the conductivity gradient and the structure gradient, and synchronous improvement of the sensitivity and the linear range is realized.

The present invention relates to the technical field of hydrogen concentration detection, and specifically discloses a trace hydrogen concentration sensing system based on TDLAS absorption spectroscopy, which is used to solve the problems of low detection sensitivity, narrow linear range, large system drift and dependence on external calibration in TDLAS hydrogen detection. The system comprises a laser emission module and a photoelectric detection module, wherein the laser emission module and the photoelectric detection module are connected via a dual-light path absorption module, the photoelectric detection module is connected to a harmonic extraction module, the harmonic extraction module is connected to a normalization processing module, and the normalization processing module is connected to a concentration inversion module. The present invention realizes high linearity, high sensitivity, and calibration-free concentration detection of hydrogen within 0.01% to 100% by constructing a symmetrical dual-light path structure, adopting a harmonic signal normalization and differential compensation algorithm, and combining a high-pressure reference gas cell with a segmented fitting model, thereby improving the trace hydrogen identification capability.

The invention discloses a flexible three-dimensional force tactile sensor and a preparation method thereof, and belongs to the technical field of flexible tactile sensors. The flexible three-dimensional force tactile sensor solves the problem that when an existing flexible three-dimensional force tactile sensor senses tangential force, the wide linear range and the high sensitivity contradict each other, and the requirements that the sensitivity and the linear range can be flexibly adjusted and customized are met. According to the invention, the sensitive layer with transverse gradient change of modulus is constructed in a manner of sleeving and splicing materials with different moduli, so that the sensitivity of the sensitive layer is changed transversely, and the sensitive layer is used for regulating and controlling the change of the sensitivity and the linear range when the sensor detects tangential force; the non-uniformity of the surface stress distribution of the sensitive layer under the action of the tangential load is utilized by utilizing the sensitivity of the non-uniform transverse distribution of the sensitive layer, so that the tangential force sensing performance of the device is further improved, the method is also effective under the normal load, and the three-dimensional force sensor prepared by the method has high sensitivity and wide linear range, and can be applied to the field of sensor devices. The method is widely applied to the aspects of three-dimensional force measurement, direction control and the like.

The invention relates to the technical field of hydrogen concentration detection, and particularly discloses a TDLAS (tunable diode laser absorption spectroscopy) absorption spectrum-based trace hydrogen concentration sensing system, which is used for solving the problems of low detection sensitivity, narrow linear range, large system drift and dependence on external calibration in TDLAS hydrogen detection, and comprises a laser emission module and a photoelectric detection module, the laser emission module and the photoelectric detection module are connected through the dual-optical-path absorption module, the photoelectric detection module is connected with a harmonic extraction module, the harmonic extraction module is connected with a normalization processing module, and the normalization processing module is connected with a concentration inversion module; according to the device and the method, a symmetrical double-light-path structure is constructed, a harmonic signal normalization and differential compensation algorithm is adopted, and a high-pressure reference gas pool and a segmented fitting model are combined, so that high-linearity, high-sensitivity and calibration-free concentration detection of hydrogen within 0.01-100% is realized, and the hydrogen trace recognition capability is improved.

The invention provides a detection method of an end face integrated reflection type SPR optical fiber biosensor manufactured based on a 2PP technology. An experiment system mainly comprises a single-mode optical fiber, an optical fiber coupler, an SPR probe, a tungsten lamp light source, a sample pool, a spectrum analyzer and a computer. The method is characterized in that a volcanic three-dimensional microstructure is directly constructed on the end face of a single-mode fiber through a 2PP technology, a metal film with the thickness of about 50nm is deposited on the inner wall of the volcanic three-dimensional microstructure, and a stable and efficient SPR excitation structure is formed; the structural parameters are subjected to FDTD simulation optimization design, and have the characteristics of high quality factor and high sensitivity. The sensor can be used for AFP detection, the sensitivity reaches 0.684 nm / (ng / mL) within the linear range of 0-10 ng / mL, the normal and risk early warning concentration interval of human serum AFP is covered, and the sensor has good biological suitability and early hepatocellular carcinoma screening capacity; in addition, the probe structure is small in size, detection can be completed only by a few microliters of liquid to be detected, and the probe is suitable for a microfluidic platform with an extremely low sample size or a clinical sampling scene. Compared with the prior art, the device has the advantages of high structural integration, high sensitivity, low sample consumption, convenience in manufacturing, stability, reliability and the like.

The invention provides a photometric device, a sample analyzer and a sample detection method. The sample analyzer comprises a loading device, a reaction device; a detection device; the controller obtains the photon counting result of each detection assembly and obtains a target counting result according to the photon counting results of the at least two detection assemblies, the linear ranges of the at least two detection assemblies are at least partially not overlapped, the photoelectric detectors of the at least two detection assemblies receive optical signals emitted by the reaction liquid in the same sample container, and the target counting result is obtained according to the optical signals. The photon counting result obtained by final detection can be prevented from being influenced by the pulse accumulation effect, so that the upper limit of the linear photometric range of the chemical reaction is improved, and the detection accuracy and the detection efficiency of a relatively strong light section of the chemical reaction are improved.

The invention provides a method for specifically detecting AFP based on a photoinduced electron transfer effect between blood orange RCDots and an AFP-aptamer, and the preparation and AFP detection principles of a near-infrared PET fluorescent probe for AFP detection are as follows: the RCDots with rich carboxyl groups on the surface can strongly adsorb ssDNA to the surface of the RCDots through pi-pi stacking interaction; and a formed RCDots / AFP-aptamer compound is subjected to a photo-induced electron transfer effect, so that the fluorescence of the RCDots is remarkably quenched. However, the affinity between the AFP and the AFP-aptamer is stronger than pi-pi stacking interaction, so that ssDNA is separated from the surface of the RCDots, the light-induced electron transfer effect disappears, the fluorescence of the RCDots is recovered, and the fluorescence intensity is changed along with the change of the concentration of the AFP, so that the concentration of the AFP is detected with one kind of specificity. The detection method is simple, has the characteristics of wide detection linear range, low detection limit, good specificity and the like, and can be widely applied to clinical detection of liver cancer markers.

The invention relates to a piezoelectric-piezoresistive collaborative mechanism-based sensor array and a preparation method and application thereof. The sensor array comprises a flexible packaging substrate layer, a piezoelectric layer, a piezoelectric layer and a piezoelectric layer which are sequentially integrated from bottom to top, the bottom electrode layer is composed of a stretchable silver electrode array subjected to silk-screen printing and serves as a lower electrode of the piezoelectric sensor; the piezoelectric functional layer is composed of a lead zirconate titanate-glass fiber composite film, and the piezoelectric functional layer is subjected to corona polarization treatment; the piezoresistive sensing layer is formed by spraying a polyvinylpyrrolidone modified carbon material dispersion liquid, the piezoresistive sensing layer is used as an upper electrode of the piezoelectric sensor, the piezoresistive sensing layer is directly deposited on the surface of the piezoelectric functional layer, and the piezoresistive sensing layer and the piezoelectric functional layer pass through a common electrode; and packaging a protection layer on the top layer. Compared with the prior art, the method has the advantages of high sensitivity, wide linear range and multi-parameter synchronous detection capability, high-resolution real-time deformation identification can be realized, and key technical support is provided for high-precision sensing application such as profile inversion.

The invention provides a wide-spectrum dual-photoelectrode-based photoelectrochemical sensor and a preparation method and application thereof, and belongs to the technical field of photoelectrochemical sensors. According to the photoelectrochemical sensor provided by the invention, a BCN / ITO electrode is used as a photoanode, a CuInS2 / ITO electrode is used as a photocathode, BCN is Bi2S3 / C3N4, and ITO is indium tin oxide. The sensor is used for detecting enrofloxacin and ciprofloxacin pollution. According to the present invention, the surface is modified with the aptamer having the specific recognition function, the specific binding effect of the aptamer with the enrofloxacin and the ciprofloxacin is utilized to form the complex of the aptamer and the target object, and the influence of the caused steric hindrance effect on the photoelectric signal and the dual-channel strategy are utilized to achieve the simultaneous detection of the residual marker; the linear ranges for detecting enrofloxacin and ciprofloxacin are 0.1 nM to 1 mu M and 50 pM to 0.5 mu M respectively, the detection limits are 24 pM and 15.5 pM respectively, and the method is successfully applied to simultaneous detection of residual markers in water. The method has the advantages of greenness, environmental protection, wide detection range, high sensitivity, good selectivity and high stability.

The invention relates to a lanthanide oxide electrochemiluminescence aptamer sensor with dual signal amplification for detecting acetamiprid as well as a preparation method and application of the lanthanide oxide electrochemiluminescence aptamer sensor. According to the sensor, terbium-doped europium oxide (Eu2O3: Tb < 3 + >) modified by gold nanoparticles (Au NPs) is used as a luminous body, and the electrochemical luminescence performance can be remarkably improved through multiple mechanisms: on one hand, the Au NPs can effectively promote generation of more sulfate free radicals; on the other hand, the surface defect passivation effect and the efficient synergistic effect of energy transfer jointly achieve effective amplification of signals. In addition, the wool ball-shaped Ag NPs is used as a plasma resonance signal amplification source, so that the ECL intensity of the sensor is further enhanced. The prepared electrochemical luminescence sensor with the dual signal amplification effect is used for detecting acetamiprid (ACE), has a wide linear range and a low detection limit, and has a wide application prospect in detection of acetamiprid residues in a water environment.

The invention belongs to the technical field of biological analysis and detection, and particularly relates to a photoelectrochemical-colorimetric dual-mode biosensor and application thereof in miRNA detection. According to the biosensor prepared by the invention, the bifunctional CuO-Cu2O nanoflower and the three-dimensional network g-C3N4 / AuNPs electrode are integrated, and an enzyme-assisted strand displacement signal amplification mechanism is combined, so that dual-mode collaborative detection of a target miRNA-21 is realized: in a photoelectrochemical mode, the detection limit reaches 0.35 fM (the linear range is 1 fM to 10 fM), and response is performed based on photocurrent polarity reversal; the colorimetric mode is as follows: the detection limit is 3.9 fM (the linear range is 10 fM to 10 nM), and TMB (Tetramethylbenzidine) color development visualization analysis is carried out. Single-mode interference is effectively eliminated through double-signal independent output and cross validation, and the method is suitable for high-reliability detection of trace miRNA (such as a breast cancer marker miRNA-21) in complex samples such as serum. According to the invention, the defects of insufficient sensitivity (pM level), poor anti-interference performance and high platform complexity in the prior art are overcome, and an efficient tool is provided for early diagnosis of cancers.

The invention discloses a strabismus mode ground interference source positioning method and system based on a satellite platform, a storage medium and electronic equipment, and the method comprises the following steps: firstly, carrying out the range pulse compression of a received interference signal according to the related parameters estimated by the prior information of an orbit and the time-frequency relation; constructing a matched filter, realizing linear range migration correction, and eliminating a first-order component of range migration in a range frequency domain-azimuth time domain; a CTF search algorithm is used, and under different search distances, distance direction search is achieved through a cooperation mechanism of global search and step length constraint local optimization; constructing matched filters at different distances to realize quadratic term range migration correction, and eliminating a range migration quadratic term in a two-dimensional frequency domain; azimuth distance compression is carried out at different distances, so that signals are focused; and obtaining a two-dimensional positioning position opposite to the interference source through coordinate transformation. According to the invention, the problem of signal defocusing caused by the distance-orientation strong coupling effect is solved in the squint mode, and the interference source positioning precision is significantly improved.

The invention discloses a construction method of an aptamer modified photonic crystal sensor for visually detecting oxytetracycline, and belongs to the technical field of sensing. In the invention, a photonic crystal array obtained by a vertical evaporation self-assembly method is combined with polyacrylamide hydrogel to form the photonic crystal hydrogel membrane material. Gold nanoparticles serve as a bridge, one side of each gold nanoparticle is connected with an amide framework of the hydrogel through chelation, the other side of each gold nanoparticle is connected with the oxytetracycline aptamer through covalent bonding, and the aptamer modified photonic crystal sensor is constructed. The oxytetracycline aptamer and oxytetracycline are highly selectively combined through pi-pi stacking and hydrogen bonds, so that the hydrogel swells, the lattice spacing of the photonic crystal is increased, a reflected signal and a structural color are subjected to red shift, and high-selectivity detection of the oxytetracycline is realized. The rapid detection requirements in the fields of food safety, environment monitoring, biological medicine and the like are met. The linear range of oxytetracycline detection is 0.1 mu g / mL-80 mu g / mL, and the lowest detection limit is 0.033 mu g / mL.

The invention relates to the technical field of angle measurement, and discloses a magnetoresistive element, an angle sensor and electronic equipment. The magnetoresistive element comprises a plurality of magnetoresistive sub-elements, and the magnetoresistive sub-elements are used for inducing in-plane magnetic field components acting on the magnetoresistive sub-elements in a magnetic field generated by the magnetic field source and outputting a linearly changing first signal in response to the in-plane magnetic field components within a preset magnetic field range; the magnetoresistive sub-element comprises a plurality of magnetic tunnel junctions, and sensing layers in the magnetic tunnel junctions are provided with closed vortex magnetization patterns; and the plurality of magnetic orbital transfer elements are positioned around the magnetic resistance sub-elements and are used for adjusting the magnetic field distribution of the magnetic field generated by the magnetic field source around the magnetic resistance sub-elements, so that the first signal is related to the rotation angle of the magnetic field source. The magnetoresistive element can measure the angle information of the magnetic field source in a linear interval, avoids the problem that a strong magnetic field generated by the magnetic field source causes fine offset of the reference layer and further affects the angle measurement precision, and has the advantages of being simple in structure and easy to implement.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The invention discloses an underwater CSK-OFDM modulation and detection method based on LED linear constraint, and the method comprises the steps: enabling a transmitter to map a data bit stream of a constellation diagram into a complex symbol stream, converting the complex symbol stream into a real number time domain sequence through an ACO-OFDM module, carrying out the LED amplitude limiting of the time domain sequence, and obtaining a time domain clipping sequence; transmitting the time domain clipping sequence in a UOWC channel through a corresponding LED, introducing symbol timing deviation and amplitude limiting noise into a frequency domain by a receiver, and processing the time domain clipping sequence by using an ACO-OFDM module again to obtain a received signal; under the maximum expectation framework, the probability from each alternative constellation diagram is calculated, symbol timing deviation and additive white Gaussian noise variance are estimated, a globally optimal solution is obtained, and a constellation diagram recognition result is output through maximum likelihood demodulation. According to the method, the linear range constraint of the LED and the complex environment under the UOWC scene are comprehensively considered.

The invention discloses a three-dimensional magnetic sensor based on an abnormal Hall effect, and belongs to the technical field of spin electron sensors. The three-dimensional magnetic sensor comprises a substrate, an AHE thin film material layer with PMA, a rare earth material layer, a protective layer and a metal electrode layer which are sequentially arranged from bottom to top, the AHE thin film material layer with PMA is of a Pt / Co / Pt multi-layer thin film heterostructure, and the rare earth material layer is Dy. According to the three-dimensional magnetic sensor based on the abnormal Hall effect provided by the invention, a rare earth material Dy layer with a proper thickness grows on a Pt / Co / Pt structure, Co and Dy form antiferromagnetic coupling based on RKKY interaction, residual magnetism adjustment driving current and power consumption are reduced, so that magnetic hysteresis is reduced, and the driving current and power consumption are correspondingly reduced; and the existence of RKKY interaction does not change the multilayer film PMA, so that the linear range is kept unchanged.

The invention relates to a protective circuit (1) for a device or power circuit breaker comprising, • an active controllable component (B1) which can be controlled in a linear range, • at least one measuring device (S P , S U , S i , S Uin , S Uout ) to determine a power output that is implemented in the actively controllable component (B1), • a processing unit (uC), • where an energy equivalent can be determined based on the specified power and a time between two measurements, • where time-separated energy equivalents are combined by the processing unit (uC), • where at least two time-related limit values ​​(G t1 , G t2 , ... G tn ) are planned, • where, upon reaching and / or exceeding a limit value (G t1 , G t2 , ... G tn) by an energy equivalent or the combined energy equivalents in a limit value (G t1 , G t2 , ... G tn ) assigned time the active controllable component (B1) is controlled by the processing unit (uC) in such a way that the current flow through. Furthermore, the invention relates to a method.

The invention discloses a perchlorate selective electrode based on an electron / ion dual-channel material and a preparation method of the perchlorate selective electrode. The electron / ion dual-channel material is synthesized by polymerizing part of polypyrrole on the surface of Mxene and introducing long-chain alkyl substituted quaternary ammonium salt, and the material combines the dual-channel characteristics of ion enrichment and conductivity, and can significantly improve the perchlorate detection capability. Experimental results show that a perchlorate selective electrode made of the dual-channel material shows extremely high selectivity and ultra-low detection limit recognition on perchlorate, the lowest detection limit can reach 12.4 mu g / L, meanwhile, the response time is extremely short (-4s), and therefore sensitive and rapid detection on the perchlorate under the complex water body condition can be achieved. The perchlorate selective electrode provided by the invention has the characteristics of wide detection linear range, low detection limit, strong anti-interference capability, simplicity in operation, high efficiency, rapidness, low cost and the like when being used for detecting perchlorate. Therefore, the method has a wide application prospect.

The present invention discloses a multi-exposure, multi-brightness, dual-parameter analysis and detection method and device, belonging to the field of immunochromatographic analysis and detection. The analysis and detection method includes obtaining a multi-exposure, multi-brightness image set under multiple multi-exposure time sequences and different light intensities. In the process of restoring the camera response function of the image acquisition system, an HDR algorithm based on multi-exposure and multi-brightness fusion is used. The weights of images with different exposures and different current values ​​are adjusted by adopting a segmented weight function. By fine-tuning the parameters of the segmented weight function to more accurately reflect the characteristics of each concentration segment in the immunochromatography, an optimal weight function is established to enhance the accuracy of the dual-parameter camera response function fitting, thereby restoring the highest-quality immunochromatographic HDR image and improving the linear range of quantitative detection.

This invention discloses a method and application for constructing a dual-mode sensor for detecting diphenyl phosphate using smartphone-assisted surface molecular imprinting, belonging to the fields of biochemical analysis, functional nanomaterials, and life science technology. In the synthesis process, the solubility, selectivity, and photoinitiation efficiency of the target analyte during polymerization are optimized, and molecularly imprinted nanozymes (MIPs) for diphenyl phosphate are rapidly prepared using molecular imprinting technology. In the detection process, the peroxidase-like activity of Fe,N-CDs catalyzes the colorimetric reaction between H₂O₂ and 3,3,5,5-tetramethylbenzidine (TMB), and the internal filtering effect of oxTMB suppresses the fluorescence signal. Combined with a smartphone, dual-signal cross-validation is achieved, constructing a colorimetric-fluorescence sensor for detecting diphenyl phosphate. This sensor exhibits specific recognition selectivity and binding affinity for diphenyl phosphate, is simple to operate, and low in cost. It can achieve sensitive detection of diphenyl phosphate over a wide linear range, with a detection limit as low as 16 pg / mL, making it suitable for on-site analysis of trace DPH in complex aquatic environments.

The invention discloses a method for high-precision detection of carbon stable isotope and content thereof in an environmental sample. The method provided by the invention comprises the following steps: detecting environmental sample powder by using an elemental analysis-stable isotope mass spectrometer; the sampling amount of the environmental sample powder is greater than or equal to 0.4 mg; in the detection process, inert gas is introduced into an opening flow divider in a Conflo universal interface of the elemental analysis-stable isotope mass spectrometer, and the inert gas is mixed with sample gas obtained by combustion in an elemental analyzer. According to the method provided by the invention, the situation that a sample signal is too high and exceeds a linear range is avoided, the representativeness of the sample is improved, the possibility of data abnormity caused by nonuniformity of the sample is reduced, and the aim of rapidly and accurately detecting the stable carbon isotope and the content of the stable carbon isotope at the same time is fulfilled.

According to the self-powered photoelectrochemical sensor for tetracycline sensitive detection and degradation, firstly, a CuBi2O4 / MoSe2 heterojunction with good photoelectric response is successfully prepared by adopting an in-situ generation method, and relatively strong and stable initial photocurrent is generated; under optimized experimental conditions, the linear range of the sensor is 1nM-10uM, the detection limit is 0.2 nM, and meanwhile, the degradation rate of the sensor to tetracycline within 30min reaches 93%. The photoelectrochemical sensor shows excellent stability, high response speed, high selectivity and good detection reproducibility. According to the invention, a synergistic effect of a detection signal and a photocatalytic degradation function is realized through integrated design, an innovative strategy is provided for real-time monitoring and synchronous efficient degradation of tetracycline pollutants, and development of a photoelectric detection-catalytic degradation integrated platform and innovative application of the photoelectric detection-catalytic degradation integrated platform in the field of environmental governance are promoted.

The invention relates to a method and a device for expanding the linear range of a detection reagent. The method comprises the following steps: applying to a finished product sample analyzer and an immunodetection kit, obtaining a first detection mode, detecting a sample according to the first detection mode to obtain first calculation information, constructing a second detection mode according to the first detection mode, and detecting the sample according to the second detection mode to obtain second calculation information, the first calculation information is adopted to calculate and obtain concentration data in the linear range of the detection reagent, the first calculation information and the second calculation information are adopted to jointly calculate and obtain concentration data outside the linear range of the detection reagent, and the ratios of the dosage of the sample to the dosage of the reagent in the first detection mode and the second detection mode are different. According to the scheme provided by the invention, on the premise of not changing reagent components, concentration data outside a linear range can be quickly and accurately obtained through result operation, and the accuracy and efficiency of sample detection are improved.

The invention provides a method for detecting the content of zinc in a lithium-zinc binary alloy strip based on ICP-OES, and belongs to the technical field of metal material component analysis. According to the method, the lithium matrix is separated by adopting a nitric acid-perchloric acid digestion system, and the stock solution is diluted by 500 times, so that the concentration of the lithium matrix can be efficiently reduced, meanwhile, the signal inhibition effect is effectively eliminated by combining lithium salt matching, and the detection precision is greatly improved. According to the method, Zn 213.856 nm analysis spectral lines are carefully selected, RF power is optimized, spectral interference of other elements can be avoided, the linear range can be expanded, and the method is suitable for rapid determination of the zinc content of 1%-20%. According to the method, the detection limit can reach 0.01%, the relative standard deviation is smaller than or equal to 1.0%, the recovery rate is 98.5%-101.2%, and high-precision detection is achieved.

The present invention discloses an exosome fluorescence detection kit and application based on in-situ initiated ARGET ATRP signal amplification. The kit includes: carboxyl magnetic beads, BSA, EDC, NHS, BMP, EGFR antibody, Apt, FA, AA, CuBr2, ME6TREN, DMSO, and PBS buffer. The kit significantly improves the detection sensitivity based on the fluorescence performance of FA and the in-situ initiated ARGET ATRP signal amplification strategy. Through calculation, it is found that there is a good linear relationship between the logarithm of exosome concentration in the range of 5×10 4 ~5×10 9 exosomes / mL and the fluorescence signal intensity. The linear equation is: F(au) = 35956lgC exosomes -118474, and the correlation coefficient (R 2 ) is 0.997. The detection limit (LOD) of this method for detecting exosomes is 1.161×10 4 exosomes / mL (S / N = 3). Compared with the linear range and detection limit for detecting exosomes in other literatures, the fluorescence signal detection kit designed by the present invention has a wider detection range and a lower detection limit.

This invention provides a method for fabricating a tactile sensing unit capable of independently adjusting sensitivity and linear range, belonging to the fields of flexible wearables, tactile sensing, and human-computer interaction. The method includes the following steps: S1, determining the structure of the tactile sensing unit; S2, establishing a correlation model between the structural parameters of the bi-phase constrained elastomer and the range and sensitivity of the sensing unit; S3, based on the target linear range and target sensitivity, obtaining the structural parameters of the high-stiffness outer ring and the negative Poisson's ratio inner ring through the correlation model; S4, fabricating the bi-phase constrained elastomer according to the structural parameters of the high-stiffness outer ring and the negative Poisson's ratio inner ring, and assembling and packaging it. This invention achieves synergistic optimization of wide linear range and high sensitivity through the bi-phase constrained elastomer structure, and supports reverse design with calculable parameters, manufacturable structure, and integrable functions based on target performance indicators.

This invention relates to the field of pressure (0~200 kPa) detection, and more particularly to MEMS integrated pressure sensor devices or monolithically integrated multi-frequency capacitive micromechanical ultrasonic transducers (CMUTs) for wide-range pressure measurement. The monolithically integrated multi-frequency CMUTs integrate a first CMUT, a second CMUT, and a third CMUT on a single chip. By adjusting the diaphragm thickness, cavity height, diaphragm radius of the first, second, and third CMUT units, as well as the pressure value within the cavity of the second CMUT unit, the first and second CMUT units operate within a linear range of small deflection deformation, and the third CMUT unit operates within a linear range of large deflection deformation. Furthermore, the operating ranges of the first, second, and third CMUT units constitute a continuous and complete measurement interval, with resonant frequencies that do not overlap, thus realizing an integrated pressure sensor device usable for measuring the entire pressure range of 0~200 kPa.