This invention discloses a method and application for constructing a dual-mode sensor for detecting
diphenyl phosphate using smartphone-assisted surface
molecular imprinting, belonging to the fields of biochemical analysis, functional
nanomaterials, and life science technology. In the synthesis process, the
solubility, selectivity, and photoinitiation efficiency of the target
analyte during
polymerization are optimized, and molecularly imprinted nanozymes (MIPs) for
diphenyl phosphate are rapidly prepared using
molecular imprinting technology. In the detection process, the
peroxidase-like activity of Fe,N-CDs catalyzes the colorimetric reaction between H₂O₂ and 3,3,5,5-tetramethylbenzidine (TMB), and the internal filtering effect of oxTMB suppresses the
fluorescence signal. Combined with a smartphone, dual-
signal cross-validation is achieved, constructing a colorimetric-
fluorescence sensor for detecting
diphenyl phosphate. This sensor exhibits specific recognition selectivity and binding affinity for diphenyl
phosphate, is simple to operate, and low in cost. It can achieve sensitive detection of diphenyl
phosphate over a wide
linear range, with a
detection limit as low as 16 pg / mL, making it suitable for on-
site analysis of trace DPH in complex aquatic environments.