38 results about "Acetamiprid" patented technology

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The invention discloses a method for detecting pesticide based on a colorimetric sensing array of a copper-based MOF composite material, and relates to the technical field of pesticide detection.The method comprises the steps that a Co, Zn and Cu-MOF (VB2) composite nano-enzyme material with peroxidase-like activity and laccase-like activity is prepared; before and after a pesticide analyte to be detected is added, the peroxidase-like activity and laccase-like activity of the Co, Zn and Cu-MOF (VB2) composite nano-enzyme material catalyze different substrates in a neutral environment, so that catalytic products have different colorimetric responses to detect the pesticide analyte to be detected. The method can be used for simultaneously distinguishing and detecting five pesticides including carbendazim, pendimethalin, thiophanate-methyl, triadimefon and acetamiprid, and has the advantages of simplicity and convenience in operation and high detection speed.

The invention discloses a butenolide composition. The butenolide composition comprises butenolide and one or more insecticides. According to the invention, butenolide is mixed with insecticides such as imidacloprid, acetamiprid, spirotetramat, flonicamid or lambda-cyhalothrin for use, so that a relatively good insecticidal synergistic effect is realized. Compared with a single pesticide preparation, the pesticide preparation containing the butenolide composition has the advantage that the prevention effect on cotton aphids is remarkably improved.

The application provides a soluble liquid agent containing flonicamid and acetamiprid, and a preparation method and application thereof. The soluble liquid agent is composed of flonicamid, acetamiprid, a solvent, a cosolvent, a surfactant, a pH regulator, a penetration enhancer and ethanol. The application reduces ecological toxicity by selecting suitable auxiliaries, has the advantages of small drift, slow evaporation, strong adhesion, good spreading, wetting and adhesion effects on targets, long retention time, rainwater erosion resistance, and improved pesticide penetration, so that the pesticide can act faster. In addition, the soluble liquid agent prepared by the application can meet the requirements of pest control under different water qualities and meet the requirements of field application under complex climate conditions. The soluble liquid agent of the application is an agricultural insecticide with good insecticidal effect, simple production process and no environmental pollution.

The present invention relates to solid water dispersible compositions comprising particulate acetamiprid and paniculate cyantraniliprole characterized by spontaneous and fast disintegration, producing physically stable suspensions of particulate cyantraniliprole in aqueous solutions of acetamiprid, with low agglomeration formation or particle size growth and minimal persistent foam. The present invention also relates to methods of use and process of preparing the solid composition comprising acetamiprid and cyantraniliprole.

The invention belongs to the technical field of food safety and rapid detection, and discloses an acetamiprid residue three-signal (colorimetric, photo-thermal and fluorescent) synergistic detection method based on aptamer and pH response double-gated nanocapsules. According to the invention, UiO-66 is used as a nano container for packaging a signal molecule TMB, and an aptamer (Apt) and chitosan (CS) are assembled on the surface of UiO-66, so that the nano capsule (UiO-66 (at) TMB (at) Apt (at) CS) which has dual response to the aptamer and pH and can accurately release TMB is constructed. The method comprises the following steps: under an acidic condition, after a target object is added, CS cracking and specific binding of Apt and the target object lead to release of TMB, firstly, HRP and H2O2 are added, and then colorimetric detection is carried out; then, irradiating the solution subjected to color development by using 808 nm laser, and carrying out photo-thermal detection; finally, a proper amount of QDs is added for fluorescence detection, and three-signal detection of acetamiprid residues is achieved.

The application belongs to the technical field of food safety rapid detection, and discloses a fluorescence and colorimetric dual-signal biosensor for acetamiprid detection, a preparation method and application thereof.The application uses aptamer (Apts) functionalized Fe3O4@Au@Pt (Fe3O4@Au@Pt@Apt) and fluorescence-labeled (FAM, 6-carboxyfluorescein) aptamer complementary strand (cDNA) functionalized SiO2 (SiO2@cDNA-FAM) as a recognition probe, uses specific recognition of the aptamer to a target, and uses enzyme catalytic activity of Fe3O4@Au@Pt and quenching effect of the fluorescence, and cooperates with a magnetic separation technology to construct a colorimetric and fluorescence dual-signal output high-sensitivity aptamer sensor for acetamiprid detection.

The invention discloses an aggregation-induced emission immunochromatography test strip as well as a preparation method and application thereof, and belongs to the technical field of residual pesticide detection. According to the present invention, by using the strong fluorescence characteristic of the AIE material and the autonomous movement ability of the nanometer motor, the binding efficiency of the antigen and the antibody is increased, the detection limit of the acetamiprid is reduced, the detection sensitivity is improved, and the accurate detection of the low-concentration acetamiprid residue in the fruit and vegetable can be achieved. Based on the stability of the AIE material and the motion characteristics of the nano motor, the influence of impurities in the fruit and vegetable matrix on a detection system can be reduced, and it is ensured that the detection performance can be stably exerted in different fruit and vegetable samples. The detection reaction is accelerated by means of active movement of the nano motor, the detection time can be greatly shortened, complex instruments and professional operation are not needed, and the method is suitable for rapidly screening acetamiprid residues in fruits and vegetables in field, market and other field scenes.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a working electrode for enhancing electrochemiluminescence performance based on a catalyst, a preparation method of the working electrode and an aptamer sensor for acetamiprid detection. The working electrode for enhancing the electrogenerated chemiluminescence property based on the catalyst is obtained by sequentially dispensing an activated europium-based MOF solution, a cDNA1 solution, a bovine serum albumin solution, a target mixture and a ZiF-67-cDNA2 solution on a glassy carbon electrode, according to the present invention, the nano confinement effect and the oxidation reduction mediation effect of ZIF-67 synergistically improve the electrochemiluminescence signal intensity, and an electrochemiluminescence enhancement strategy using ZIF-67 as the catalyst is provided, such that during acetamiprid detection, the enrichment and the reaction kinetics of active free radicals are significantly promoted through the ternary synergistic effect of ZIF-67, europium-based MOF and tripropylamine so as to significantly improve the detection sensitivity of acetamiprid. Therefore, the sensitivity of the sensing system is improved, and new technical support is provided for agricultural product safety monitoring.

This invention provides an emulsifiable concentrate formulation comprising the active ingredients chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole, wherein the weight ratio of chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole is 1.2:17.8:12. It also includes Silway 241 and Atlox 4916, wherein the mass ratio of Silway 241 to Atlox 4916 is 0.1~0.15:3. The emulsifiable concentrate formulation provided by this invention has strong spreading and adhesion capabilities, which can improve the effective utilization rate of the herbicide solution, exhibit excellent control effects on weeds, and can slow down the development of weed resistance.

The invention relates to a sanitary insecticide, in particular to a bug insecticidal composition containing afluthrin and application of the bug insecticidal composition. The composition comprises an active component A and an active component B in a weight ratio of (1: 20)-(20: 1), wherein the active ingredient A is afluthrin; and the active ingredient B is dinotefuran, acetamiprid, imidacloprid, thiacloprid, thiamethoxam, flupyradifurone or sulfoxaflor. The composition provided by the invention not only solves the problem of resistance of bed bugs to pyrethroid, but also improves the pesticide effect and pesticide effect stability of the composition through the synergistic interaction among the active components, and has the characteristics of high efficiency, low toxicity, environmental friendliness and the like. The binary composition is suitable for preventing and treating bugs in indoor activity places such as families, carriages, hotels, factory dormitories and the like.

This invention belongs to the field of photoelectrochemical sensing materials technology, specifically relating to a composite material based on Sb₂S₃ / CaIn₂S₄, its preparation method, and its application. The composite material includes an ITO electrode and Sb₂S₃ / CaIn₂S₄ composited on its surface, wherein Sb₂S₃ and CaIn₂S₄ form a type II heterojunction. This invention also provides an in-situ hydrothermal preparation method for this composite material. By dividing the composite material into multiple regions using insulating stickers and assembling Au@LaNiO₃-Apt signal markers, an optically addressable photoelectrochemical sensor array can be obtained. This sensor array is used for high-throughput sensitive detection of acetamiprid. This invention effectively suppresses carrier recombination through the type II heterojunction, significantly enhancing the photoelectrochemical response and solving the problems of insufficient sensitivity and limited high-throughput detection capability of existing photoelectrochemical sensors.

The application discloses a kind of fluorescence methods for detecting acetamiprid on site based on nano magnetic beads, belong to analytical chemistry and biotechnology field.The method will be modified with biotin acetamiprid specific aptamer Apt probe fixed on the surface of modified with streptavidin magnetic beads MBs, when acetamiprid ACE exists, Apt and acetamiprid are combined to form stable MBs-Apt-ACE complex.Subsequently, labeled with fluorescent quantum dots QD cDNA is added, Apt that has been combined with acetamiprid cannot be combined with cDNA again, at this time, magnet is used to remove MBs, and the supernatant is detected by fluorescence.The higher the concentration of acetamiprid, the greater the fluorescence intensity of supernatant, so as to realize the detection of acetamiprid.

The invention discloses an iron-doped carbon dot nano-enzyme assisted ratio type electrochemical aptamer sensor, a preparation method and application, and belongs to the field of sensors. The method comprises the following steps: (1) preparing a molybdenum disulfide-gold nanoparticle-methylene blue composite material modified electrode; (2) preparing a MoS2 / Au / MB / cDNA modified electrode; (3) preparing the iron-doped carbon dot nano enzyme; (4) preparing the iron-doped carbon dots modified by the acetamiprid aptamer; and (5) preparing the MoS2 / Au / MB / cDNA (at) Apt / Fe-CDs modified electrode. The preparation method disclosed by the invention is low in cost, simple in experimental operation and easy in reaction condition control. The iron-doped carbon dot nano enzyme-assisted ratio-type electrochemical aptamer sensor prepared by the invention can be used as a working electrode for quantitative analysis and detection of acetamiprid, and has good stability, high sensitivity, high specificity and high accuracy in acetamiprid detection.

This invention provides a herbicidal composition comprising the active ingredients chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole, wherein the mass ratio of chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole is 1~3:15~20:10~15. The herbicidal composition has a significant synergistic effect and can effectively solve the problem of weed resistance.

The invention discloses a preparation method and application of an antigen for detecting impurities such as etoxazole in vegetables and fruits. The obtained antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting etoxazole contained in vegetables and fruits, the detection limit is 10 [mu] g / L. The detection result is high in accuracy, especially in an etoxazole antibody specificity experiment, obvious specificity to etoxazole is shown, and the detection result is not interfered by drugs such as carbendazim, triazophos, chlorpyrifos, cyfluthrin, metalaxyl, acetamiprid, difenoconazole, procymidone, chlorfenapyr, profenofos and omethoate.

Pesticidal mixtures comprising as active compounds A) the ethylsulfone compound of formula (I) and B) at least one further compound B selected from fipronil, lambda-cyhalothrin, bifenthrin, tefluthrin, alpha-cypermethrin, thiamethoxam, clothianidin, acetamiprid, imidacloprid, dinotefuran, tri-flumezopyrim, fenmezoditiaz, spinosad, spinetoram, abamectin, afidopyropen, indoxacarb; met-aflumizone, spiropidion, spidoxamat, chlorantraniliprole, cyantraniliprole, tetraniliprole, cyclaniliprole, broflanilide, isocycloseram; dimpropyridaz; and difenoconazole, prothioconazole, flutriafol, ipconazole, tebuconazole, mefentrifluconazole, triticonazole, fluoxytioconazole, metyltetraprole, azoxystrobin, trifloxystrobin, pyraclostrobin, sedaxane, penflufen, fluopyram, fluxapyroxad, pydiflumetofen, isoflucypram, cyclobutrifluram, oxathiapiprolin, fluoxapriprolin, metalaxyl, picarbutrazox, fludioxonil, thiophanat-methyl; methods and use of these mixtures for combating invertebrate pests such as insects, arachnids or nematodes in and on plants, and for protecting such plants being infested with pests, especially also for protecting plant propagation material as like seeds.

The invention provides a biomass charcoal adsorption material and a preparation method thereof, and belongs to the field of pesticide degradation. The preparation method comprises the following steps: preparing biochar, preparing modified biochar and compounding. The compounding method comprises the following steps: adding modified biochar, bacillus subtilis, pseudomonas FH2, bacillus licheniformis and phanerochaete chrysosporium into deionized water, stirring, filtering, adding a filter cake into a chitosan solution, then adding Tween 60, stirring for 2-3 hours at the speed of 500-600r / min, slowly dropwise adding a glutaraldehyde solution, continuously stirring for 8-10 hours after dropwise adding, finally centrifuging at the speed of 7000-8000r / min, and drying to obtain the biochar composite material. Collecting solids, and drying to obtain a biomass charcoal adsorption material; the biomass charcoal adsorption material disclosed by the invention has broad spectrum on pesticide degradation, the degradation rate on trichlorfon reaches 87 to 89 percent, the degradation rate on acetamiprid reaches 89 to 93 percent, the degradation rate on deltamethrin reaches 93 to 96 percent, and the degradation rate on DDT reaches 65 to 70 percent.

The invention discloses a quarantine treatment method for phoenix heterothoracic beetles, belongs to the technical field of plant quarantine, and provides a quarantine treatment method for phoenix heterothoracic beetles harmful to palm plants (coconuts, phoenix and the like). The treatment method comprises the following steps: placing the phoenix dactylifera plants to be quarantine in an environment with the temperature of 0-5 DEG C, treating for 12-24 hours, spraying an acetamiprid medicament with the concentration of 10-20 mg / L, and standing for at least 24 hours. After the treatment is completed, the phoenix dactylicapnostoma on the phoenix seedlings is detected, and the result shows that the death rate of pests reaches 98% or above, and phoenix has good growth condition and no obvious damage.

The invention discloses an electrochemical luminescence (ECL) sensor based on a luminous hydrogel and graphene oxide (GO)-aptamer interface and an acetamiprid detection method thereof. The luminescent hydrogel is formed by loading manganese dioxide nanosheets (MnO2NSs), N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and gold nanoparticles (AuNPs) grown in situ in a three-dimensional network of polyethyleneimine-glutaraldehyde (PEI-GA) together, and is used as an ECL luminescent layer with high density and stable microenvironment; a GO-aptamer composite layer is constructed on the surface of the GO-aptamer composite layer, and signal transduction of GO short-range quenching-target induced desorption-signal recovery (signal-on) is achieved. An ECL signal is collected in a buffer system containing a hydrogen peroxide co-reactant, and a quantitative relation is established by delta IECL or I / I0, so that sensitive detection of acetamiprid is realized; good linearity is realized in a range of 1.0 * 10 <-3 > to 1.0 * 10 < 3 > nM, and the detection limit is about 0.3 pM. The method is good in repeatability and stability, and can be combined with QuEChERS pretreatment to be used for sample detection of vegetables such as spinach, celery and leek.

The application discloses an aggregation-induced emission immunochromatographic test strip as well as a preparation method and application thereof, and belongs to the technical field of residual pesticide detection. The aggregation-induced emission immunochromatographic test strip utilizes the strong fluorescence characteristics of an AIE material and the autonomous movement ability of a nanomotor, increases the binding efficiency of an antigen and an antibody, reduces the detection limit of acetamiprid, improves the detection sensitivity, and can realize precise detection of low-concentration acetamiprid residues in fruits and vegetables. The aggregation-induced emission immunochromatographic test strip can reduce the influence of impurities in fruit and vegetable matrixes on the detection system based on the stability of the AIE material and the movement characteristics of the nanomotor, and can ensure stable detection performance in different fruit and vegetable samples. The aggregation-induced emission immunochromatographic test strip can greatly shorten the detection time by virtue of the active movement of the nanomotor to accelerate the detection reaction, does not need complex instruments and professional operation, and is suitable for rapid screening of acetamiprid residues in fruits and vegetables in field, market and other on-site scenes.

This invention relates to the field of compound pesticides, specifically to a dispersible liquid formulation, its preparation method, and its application. The invention provides a dispersible liquid formulation comprising an active ingredient, a polar solvent, an emulsifier, and a dispersant; the mass ratio of acetamiprid to pyriproxyfen in the active ingredient is (0.1-10):1; the relative permittivity of the polar solvent is 10-90. The dispersible liquid formulation of this invention possesses low foaming, easy dispersibility, and high suspension properties, greatly simplifying field operations, avoiding spray blockage and uneven pesticide application, and improving application efficiency and safety. The dispersible liquid formulation provided by this invention achieves a comprehensive performance balance of "low foaming, high stability, and ease of use," and has promising prospects for large-scale application.

This invention provides a method for controlling the olive psyllid, belonging to the field of olive cultivation technology. The method includes the following steps: S1: Applying organic fertilizer, superphosphate compound fertilizer, urea, and ammonium bicarbonate to the soil to promote the growth of new shoots and leaves; S2: Collecting adult psyllids, eggs, larvae, or pupae of natural enemies of the psyllid manually and releasing them to fully utilize natural control; simultaneously, spraying with highly effective and low-toxicity pesticides that have low toxicity to natural enemies; S3: Checking for pests each time new shoots emerge and spraying with pesticides, namely acetamiprid wettable powder, milbemycin, and spirotetramat; S4: Winter orchard cleanup. This invention, by spraying acetamiprid wettable powder, milbemycin, and spirotetramat during the control of the olive psyllid, achieves a synergistic effect, synergistically improving the control efficacy of the olive psyllid.

The invention relates to the technical field of chemical analysis, in particular to a method for analyzing the content of 4-(4-methylphenoxy) benzonitrile, which adopts high performance liquid chromatography for analysis and comprises the following steps: (1) preparing a standard substance solution and a to-be-detected sample solution; (2) sequentially carrying out sample introduction analysis by using a high performance liquid chromatograph according to the sequence of the standard substance 1, the to-be-detected sample 1, the standard substance 2 and the to-be-detected sample 2; and (3) calculating the average value of the peak areas of the 4-(4-methylphenoxy) benzonitrile in the standard substance and the sample to be detected, and calculating the content of the 4-(4-methylphenoxy) benzonitrile in the sample to be detected according to an external standard method formula. The analysis method has the advantages of strong specificity, good repeatability and good precision, and is especially suitable for the quality control of intermediate products in the pesticide production process, thereby meeting the production requirements of the high-quality pesticide raw pesticide tolfenpyrad.

The invention relates to an azotobacter and application thereof in repairing co-pollution of insecticides acetamiprid and sulfoxaflor, belongs to the technical field of microorganisms, and provides an Ensifer sp. DA6. Resting cells of the Ensifer sp. DA6 can degrade the insecticides acetamiprid and sulfoxaflor at the same time in phosphate buffer. Immobilized cells of the strain can effectively repair co-pollution residues of insecticides acetamiprid and sulfoxaflor in surface water; the strain can effectively repair the co-pollution residues of two insecticides, namely acetamiprid and sulfoxaflor, and a microbial remediation method is provided for repairing the co-pollution residues of the insecticides, namely acetamiprid and sulfoxaflor; meanwhile, the strain has nitrogen fixation activity, and can be used as a plant growth-promoting bacterium while repairing the co-pollution of acetamiprid and sulfoxaflor; the invention further provides a new nitrile hydratase derived from Ensifer sp. DA6, and the nitrile hydratase can degrade acetamiprid and sulfoxaflor when the acetamiprid and the sulfoxaflor coexist.

The application discloses a use and a preparation method of cobalt tin hydroxide CoSn(OH)6 for catalytic degradation of nicotine pollutants, wherein the cobalt tin hydroxide is a bimetallic hydroxide with a cubic structure and can catalyze and activate peroxymonosulfate to oxidatively degrade nicotine pesticides under normal temperature conditions, so as to remove the pollutants in water, and especially has a good degradation effect on acetamiprid (ACE).

The present application relates to the field of pesticide composition, especially to a stable and efficient pyrethrum insecticide composition and its preparation method. The composition comprises at least 1-10wt% of pyrethrum, 1-25wt% of the second active substance, 5-25wt% of functional adjuvant and the rest of solvent. The composition provided in the present application is different from the existing water dispersible granules, emulsifiable concentrate and suspension concentrate of pyrethrum and acetamiprid combination, which is a soluble concentrate composition with extremely high active ingredient content. The composition has obvious advantages in drug efficacy compared with solid preparations, and is more environmentally friendly than the existing emulsifiable concentrate type, and has excellent market prospect in the field of pesticides.

This invention discloses a colorimetric sensor for detecting pesticides, its preparation method, and its application, belonging to the field of hazardous substance detection technology. The colorimetric sensor of this invention consists of a capture probe and a signal probe; wherein the capture probe is selected from a thiol-containing nucleic acid aptamer modified with Au-SSM membrane material, i.e., SH-aptamer-Au-SSM; the signal probe is selected from a thiol-containing cDNA modified with AuPt@IL@Fe3O4 nanomaterial, i.e., SH-cDNA-AuPt@IL@Fe3O4. The colorimetric sensor of this invention can specifically identify acetamiprid, and the detection method is simple to operate, requiring no complex pretreatment of the test sample; the detection cost is low, the detection is rapid, and the requirements for detection instruments are low.

The invention discloses a self-powered electrochemical sensor for detecting acetamiprid, the self-powered electrochemical sensor comprises an anode, a cathode and an electrolyte, the anode is an AuNPs / GDY / GOD biological anode, and the cathode is an AuNPs / GDY / DNA chain biological sensor. The self-energized electrochemical sensor integrates a self-energized sensing technology, a multi-cascade nucleic acid amplification technology, capacitor auxiliary amplification and auxiliary reading by using a smart phone or a computer and the like. The sensor is used for high-sensitivity detection of acetamiprid ACE residues in food. The ACE detection method provided by the invention has the advantages of simplicity, rapidness, ultrasensitivity, intelligence and the like, has remarkable and excellent performance, and shows huge application potential in accurate monitoring of ACE in food and environment.

The invention discloses a ZIF8-based self-reinforced composite material, an electrochemiluminescence-electrochemical aptamer sensor and a preparation method and application thereof, a novel self-reinforced electrochemiluminescence composite material (Ru-N-at-ZIF8) is designed and prepared by utilizing a one-step self-assembly method, and a luminous body (Ru (dcbpy) 3 < 2 + >) and a co-reactant (NGQDs) are simultaneously wrapped in the same material (ZIF8), so that the electron transfer distance is shortened, and the electrochemical luminescence-electrochemical aptamer sensor has the advantages that the electrochemical luminescence-electrochemical aptamer sensor is more stable in performance. The electrochemical luminescence efficiency is obviously improved. The self-enhanced electrochemical luminescence-electrochemical dual-mode aptamer sensor based on the Ru-N-coated ZIF8 is used for detecting acetamiprid, the linear range of electrochemical luminescence and the linear range of electrochemistry are both 1 pM to 100 nM, and the detection limits are 0.046 pM and 0.050 pM respectively. The sensor provided by the invention shows better selectivity, and has better reproducibility and stability.