75 results about "Acetamiprid" patented technology

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The utility model relates to an acetamiprid raw material proportioning device applied to the technical field of insecticides, which comprises a device body with supporting legs, and a supporting plate is fixedly connected between the supporting legs, by adopting the mixing structure, after the acetamiprid raw material is proportioned, a temperature sensor and a servo motor can be started through a controller, and the acetamiprid raw material is proportioned. The servo motor drives the rotating shaft to drive the stirring paddle to rotate, so that the scraping plate on the stirring paddle scrapes off raw materials adhered to the inner wall of the device body, and meanwhile, the stirring brush at the bottom stirs the bottom of the device body and floats the raw materials sinking to the bottom, so that the effect of uniformly mixing the raw materials is achieved; meanwhile, the stirring paddle rotates to drive the temperature sensor on the stirring paddle to rotate, so that the temperature sensor can move in all directions to detect the temperature in the device body, and when the temperature distribution is detected to be non-uniform, the controller automatically adjusts the rotating speed of the servo motor, so that the heat transfer is uniform in the stirring process, and the problem of non-uniform temperature distribution in the reaction kettle is avoided.

The invention discloses a method for detecting pesticide based on a colorimetric sensing array of a copper-based MOF composite material, and relates to the technical field of pesticide detection.The method comprises the steps that a Co, Zn and Cu-MOF (VB2) composite nano-enzyme material with peroxidase-like activity and laccase-like activity is prepared; before and after a pesticide analyte to be detected is added, the peroxidase-like activity and laccase-like activity of the Co, Zn and Cu-MOF (VB2) composite nano-enzyme material catalyze different substrates in a neutral environment, so that catalytic products have different colorimetric responses to detect the pesticide analyte to be detected. The method can be used for simultaneously distinguishing and detecting five pesticides including carbendazim, pendimethalin, thiophanate-methyl, triadimefon and acetamiprid, and has the advantages of simplicity and convenience in operation and high detection speed.

The invention discloses a preparation method and application of a cobalt-doped biomass charcoal catalyst, and particularly relates to the preparation method of the catalyst comprising the following steps: S1, pretreatment of biomass charcoal, S2, preparation of a catalyst precursor, and S3, high-temperature calcination. According to the method, the corn straw is used as a biomass charcoal raw material, the preparation cost of the catalyst is reduced, an economic basis is provided for large-scale application of the catalyst, a degradation experiment of metalaxyl with different catalyst concentrations shows that when the concentration of the cobalt-doped biomass charcoal catalyst is increased, the degradation rate of metalaxyl is also increased, and the degradation rate of metalaxyl is increased. The traditional Chinese medicine residue is used as a biomass charcoal raw material, the traditional Chinese medicine residue is a waste obtained after traditional Chinese medicine production and is used for preparing the catalyst, resource utilization of the waste is achieved, solid waste discharge is reduced, and meanwhile three groups of experimental results show that the catalyst has efficient degradation capacity on acetamiprid.

The invention discloses a compound insecticide and application thereof, the insecticide is a soluble liquid, the insecticide comprises acetamiprid, pyridaben and auxiliary components, and the mass ratio R of the acetamiprid to the pyridaben ranges from 1: 50 to 50: 1. The insecticidal composition is used for preventing and controlling piercing-sucking mouthpart pests. The insecticidal composition can delay the drug resistance process, reduce the dosage, lower the use cost, relieve the environmental pollution and expand the insecticidal spectrum, and has the gain effect of compound addition.

The invention discloses a butenolide composition. The butenolide composition comprises butenolide and one or more insecticides. According to the invention, butenolide is mixed with insecticides such as imidacloprid, acetamiprid, spirotetramat, flonicamid or lambda-cyhalothrin for use, so that a relatively good insecticidal synergistic effect is realized. Compared with a single pesticide preparation, the pesticide preparation containing the butenolide composition has the advantage that the prevention effect on cotton aphids is remarkably improved.

The application provides a soluble liquid agent containing flonicamid and acetamiprid, and a preparation method and application thereof. The soluble liquid agent is composed of flonicamid, acetamiprid, a solvent, a cosolvent, a surfactant, a pH regulator, a penetration enhancer and ethanol. The application reduces ecological toxicity by selecting suitable auxiliaries, has the advantages of small drift, slow evaporation, strong adhesion, good spreading, wetting and adhesion effects on targets, long retention time, rainwater erosion resistance, and improved pesticide penetration, so that the pesticide can act faster. In addition, the soluble liquid agent prepared by the application can meet the requirements of pest control under different water qualities and meet the requirements of field application under complex climate conditions. The soluble liquid agent of the application is an agricultural insecticide with good insecticidal effect, simple production process and no environmental pollution.

The present invention relates to solid water dispersible compositions comprising particulate acetamiprid and paniculate cyantraniliprole characterized by spontaneous and fast disintegration, producing physically stable suspensions of particulate cyantraniliprole in aqueous solutions of acetamiprid, with low agglomeration formation or particle size growth and minimal persistent foam. The present invention also relates to methods of use and process of preparing the solid composition comprising acetamiprid and cyantraniliprole.

The application discloses application of a microbial natural product GameXPeptide-A in improving the control effect of insecticides on pests, and belongs to the technical field of biological control. Experiments prove that GameXPeptide-A can effectively improve the insecticidal activity of insecticides on agricultural pests such as thrips, corn borer and prodenia litura when GameXPeptide-A is combined with insecticides such as Metarrhizium anisopliae, Beauveria bassiana, nuclear polyhedrosis virus, 4% abamectin and pyridaben, 10% dimethoate and spinosad, 30% ethoxazole, 45% bifenthrin and etoxazole, 5% spinosad, 5% chlorantraniliprole, 5% abamectin and the like, and it is indicated that the substance can be combined with different insecticides to improve the insecticidal activity of insecticides on pests such as Lepidoptera, Diptera, Coleoptera, Orthoptera, Hemiptera and Homoptera in agricultural production, and can be used for the development and application of a new type of pest immunosuppressant or insecticide synergist.

The invention belongs to the technical field of food safety and rapid detection, and discloses an acetamiprid residue three-signal (colorimetric, photo-thermal and fluorescent) synergistic detection method based on aptamer and pH response double-gated nanocapsules. According to the invention, UiO-66 is used as a nano container for packaging a signal molecule TMB, and an aptamer (Apt) and chitosan (CS) are assembled on the surface of UiO-66, so that the nano capsule (UiO-66 (at) TMB (at) Apt (at) CS) which has dual response to the aptamer and pH and can accurately release TMB is constructed. The method comprises the following steps: under an acidic condition, after a target object is added, CS cracking and specific binding of Apt and the target object lead to release of TMB, firstly, HRP and H2O2 are added, and then colorimetric detection is carried out; then, irradiating the solution subjected to color development by using 808 nm laser, and carrying out photo-thermal detection; finally, a proper amount of QDs is added for fluorescence detection, and three-signal detection of acetamiprid residues is achieved.

The application belongs to the technical field of food safety rapid detection, and discloses a fluorescence and colorimetric dual-signal biosensor for acetamiprid detection, a preparation method and application thereof.The application uses aptamer (Apts) functionalized Fe3O4@Au@Pt (Fe3O4@Au@Pt@Apt) and fluorescence-labeled (FAM, 6-carboxyfluorescein) aptamer complementary strand (cDNA) functionalized SiO2 (SiO2@cDNA-FAM) as a recognition probe, uses specific recognition of the aptamer to a target, and uses enzyme catalytic activity of Fe3O4@Au@Pt and quenching effect of the fluorescence, and cooperates with a magnetic separation technology to construct a colorimetric and fluorescence dual-signal output high-sensitivity aptamer sensor for acetamiprid detection.

The efficient drying equipment comprises a drying drum machine, a transverse roller shaft is arranged in the middle of the inner end of the drying drum machine, the transverse roller shaft extends to the rear side of the drying drum machine, the lower end of the transverse roller shaft is fixedly connected with a rear side vertical plate, and the lower end of the rear side vertical plate is fixedly connected with an upper side vertical plate. A hollow bottom frame is installed at the upper end of the transverse roller shaft, a U-shaped ejector rod is fixedly connected to the upper end of the hollow bottom frame, springs are symmetrically and fixedly connected to the left side and the right side of the upper end of the U-shaped ejector rod, and a mesh screen plate is fixedly connected to the upper ends of the two springs; according to the scheme, the caked acetamiprid particle materials are screened through the mesh screen plate, so that the caked acetamiprid particle materials are not in the caked state any more, the acetamiprid particle materials can make uniform contact with hot air in the drying roller machine in the remaining drying time, and the heat transfer effect is guaranteed.

The invention discloses an aggregation-induced emission immunochromatography test strip as well as a preparation method and application thereof, and belongs to the technical field of residual pesticide detection. According to the present invention, by using the strong fluorescence characteristic of the AIE material and the autonomous movement ability of the nanometer motor, the binding efficiency of the antigen and the antibody is increased, the detection limit of the acetamiprid is reduced, the detection sensitivity is improved, and the accurate detection of the low-concentration acetamiprid residue in the fruit and vegetable can be achieved. Based on the stability of the AIE material and the motion characteristics of the nano motor, the influence of impurities in the fruit and vegetable matrix on a detection system can be reduced, and it is ensured that the detection performance can be stably exerted in different fruit and vegetable samples. The detection reaction is accelerated by means of active movement of the nano motor, the detection time can be greatly shortened, complex instruments and professional operation are not needed, and the method is suitable for rapidly screening acetamiprid residues in fruits and vegetables in field, market and other field scenes.

The utility model relates to the technical field of pesticide production, and discloses an acetamiprid-methylamine positive pressure absorption device which comprises a reaction kettle, the upper surface of the reaction kettle is in through connection with an electromagnetic valve A, one end of the electromagnetic valve A is in through connection with an air outlet pipe A, and one end of the air outlet pipe A is fixedly connected with a flange plate. According to the positive pressure absorption device for acetamiprid-methylamine, through the arrangement of the controller and the pressure control assembly, when the device is used, firstly, gas containing methylamine generated by the reaction kettle enters the absorption tower through the gas outlet pipe A, the guide pipe, the gas inlet pipe A and the gas distributor, the pressure sensor detects the pressure value and transmits the pressure value to the controller, the controller controls the opening and closing of the back pressure valve, and the pressure control assembly controls the back pressure valve to open and close. And secondly, the infrared temperature sensor detects the temperature in the absorption tower in real time and transmits data to the controller, and the controller adjusts the heating temperature of the heating power grid, so that the pressure and the temperature in the absorption tower can be flexibly adjusted.

The invention discloses a preparation method of a modified nano composite material and application of the modified nano composite material in degradation of new pollutants, and belongs to the field of new pollutant treatment. According to the preparation method disclosed by the invention, LaFeO3 is doped into MIL-88A to synthesize heterojunction MIL-88A-LaFeO3, and citric acid is added, so that the photocatalytic performance of the material is improved. The photo-Fenton degradation efficiency of the MIL-88A-coated LaFeO3 prepared by the preparation method disclosed by the invention on acetamiprid reaches 99.9% within 180 minutes, and the photo-Fenton degradation efficiency of the MIL-88A-coated LaFeO3 prepared by the preparation method disclosed by the invention on sulfamethoxazole reaches 100% within 180 minutes. The photo-Fenton degradation efficiency of the composite material modified by citric acid on acetamiprid reaches 100% within 75 min, and the composite material shows more excellent photocatalytic activity. The preparation method disclosed by the invention is simple in material and low in cost, and the prepared material is high in degradation rate, has broad spectrum and is applied to degradation of various types of neonicotinoid pesticides and antibiotics in a water body.

This invention belongs to the field of pesticides and insecticides, specifically relating to an insecticidal composition containing bis(triflufenoxuron) and its application. The insecticidal composition includes active ingredient A and active ingredient B. Active ingredient A is bis(triflufenoxuron), and active ingredient B is any one of dinotefuran, spinosad, flonicamid, and acetamiprid. The mass ratio of active ingredient A to active ingredient B is 1:30 to 25:1. The insecticidal composition of this invention has advantages such as synergistic effects and reduced resistance. This insecticidal composition can be used to control agricultural, forestry, and horticultural pests.

The present invention discloses a method for controlling Plutella xylostella based on a reduction-responsive nano-acetamiprid pesticide, belonging to the field of nano-pesticides. First, a mesoporous silica nano-carrier and didodecyl disulfide are prepared. Then, the mesoporous silica nano-carrier is modified with a thiol group, and the thiol group-modified mesoporous silica nano-carrier is blocked with didodecyl disulfide. Finally, it is compounded with acetamiprid to prepare a reduction-responsive nano-acetamiprid pesticide. The reduction-responsive nano-acetamiprid pesticide adopted by the present invention has good pH, temperature, light and long-term storage stability, and has broad application value in the field of controlling Plutella xylostella in farmland; moreover, the method for using the reduction-responsive nano-acetamiprid pesticide to control Plutella xylostella is simple and has strong versatility, without the need for special equipment.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a working electrode for enhancing electrochemiluminescence performance based on a catalyst, a preparation method of the working electrode and an aptamer sensor for acetamiprid detection. The working electrode for enhancing the electrogenerated chemiluminescence property based on the catalyst is obtained by sequentially dispensing an activated europium-based MOF solution, a cDNA1 solution, a bovine serum albumin solution, a target mixture and a ZiF-67-cDNA2 solution on a glassy carbon electrode, according to the present invention, the nano confinement effect and the oxidation reduction mediation effect of ZIF-67 synergistically improve the electrochemiluminescence signal intensity, and an electrochemiluminescence enhancement strategy using ZIF-67 as the catalyst is provided, such that during acetamiprid detection, the enrichment and the reaction kinetics of active free radicals are significantly promoted through the ternary synergistic effect of ZIF-67, europium-based MOF and tripropylamine so as to significantly improve the detection sensitivity of acetamiprid. Therefore, the sensitivity of the sensing system is improved, and new technical support is provided for agricultural product safety monitoring.

This invention provides an emulsifiable concentrate formulation comprising the active ingredients chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole, wherein the weight ratio of chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole is 1.2:17.8:12. It also includes Silway 241 and Atlox 4916, wherein the mass ratio of Silway 241 to Atlox 4916 is 0.1~0.15:3. The emulsifiable concentrate formulation provided by this invention has strong spreading and adhesion capabilities, which can improve the effective utilization rate of the herbicide solution, exhibit excellent control effects on weeds, and can slow down the development of weed resistance.

The present invention discloses an aqueous emulsion containing acetamiprid and lambda-cyhalothrin and a preparation method thereof. The aqueous emulsion is composed of the following components in parts by weight: 10-100 g / L of active ingredient, 60-120 g / L of emulsifier, 100-200 g / L of solvent, 50-150 g / L of cosolvent, 1-10 g / L of thickener, 0.5-2 g / L of defoamer, 3-10 g / L of antifreeze, 1-2 g / L of pH regulator, 1-2 g / L of preservative, and the balance of water; wherein, the mass ratio of acetamiprid to lambda-cyhalothrin is 1-20:1. The aqueous emulsion of the present invention has strong stability over time, can withstand the high and low temperature range of 0°C to 54°C, effectively reduces or solves the influence of environmental changes during transportation on the stability of the dosage form, reduces or avoids product quality problems such as stratification or sedimentation, flocculation, coalescence, crystal precipitation, decomposition, etc. of the dosage form, improves the stability of the preparation, and improves the insecticidal effect.

The invention relates to a sanitary insecticide, in particular to a bug insecticidal composition containing afluthrin and application of the bug insecticidal composition. The composition comprises an active component A and an active component B in a weight ratio of (1: 20)-(20: 1), wherein the active ingredient A is afluthrin; and the active ingredient B is dinotefuran, acetamiprid, imidacloprid, thiacloprid, thiamethoxam, flupyradifurone or sulfoxaflor. The composition provided by the invention not only solves the problem of resistance of bed bugs to pyrethroid, but also improves the pesticide effect and pesticide effect stability of the composition through the synergistic interaction among the active components, and has the characteristics of high efficiency, low toxicity, environmental friendliness and the like. The binary composition is suitable for preventing and treating bugs in indoor activity places such as families, carriages, hotels, factory dormitories and the like.

This invention belongs to the field of photoelectrochemical sensing materials technology, specifically relating to a composite material based on Sb₂S₃ / CaIn₂S₄, its preparation method, and its application. The composite material includes an ITO electrode and Sb₂S₃ / CaIn₂S₄ composited on its surface, wherein Sb₂S₃ and CaIn₂S₄ form a type II heterojunction. This invention also provides an in-situ hydrothermal preparation method for this composite material. By dividing the composite material into multiple regions using insulating stickers and assembling Au@LaNiO₃-Apt signal markers, an optically addressable photoelectrochemical sensor array can be obtained. This sensor array is used for high-throughput sensitive detection of acetamiprid. This invention effectively suppresses carrier recombination through the type II heterojunction, significantly enhancing the photoelectrochemical response and solving the problems of insufficient sensitivity and limited high-throughput detection capability of existing photoelectrochemical sensors.

The invention discloses a broad-spectrum insecticide based on RNA interference technology and a preparation method thereof, and relates to the technical field of broad-spectrum insecticides, the preparation method comprises the following steps: S1, material selection; s2, preparing double-stranded siRNA (small interfering Ribonucleic Acid); s3, preparing hyaluronic acid; s4, preparing an acetamiprid solution; s5, mixing; and S6, packaging a finished product. According to the broad-spectrum insecticide based on the RNA interference technology and the preparation method of the broad-spectrum insecticide, firstly, preparation raw materials of double-stranded siRNA, hyaluronic acid and an acetamiprid solution are prepared respectively, so that three mixed materials can be formed, then the three mixed materials are put into a mixing tank to be uniformly mixed to form a uniform compound solution, and the compound solution is added into the mixing tank to prepare the broad-spectrum insecticide based on the RNA interference technology. The preparation method comprises the following steps: mixing a plurality of substances in a compound solution, then filling and packaging the solution after mixing is finished so as to facilitate subsequent selling and transportation use, and because the double-stranded siRNA in the prepared compound solution is a double-stranded RNA molecule and can specifically bind and degrade specific mRNA in cells, the expression of a target gene is inhibited, and a key gene in a pest body can be effectively silenced.

The application discloses a kind of fluorescence methods for detecting acetamiprid on site based on nano magnetic beads, belong to analytical chemistry and biotechnology field.The method will be modified with biotin acetamiprid specific aptamer Apt probe fixed on the surface of modified with streptavidin magnetic beads MBs, when acetamiprid ACE exists, Apt and acetamiprid are combined to form stable MBs-Apt-ACE complex.Subsequently, labeled with fluorescent quantum dots QD cDNA is added, Apt that has been combined with acetamiprid cannot be combined with cDNA again, at this time, magnet is used to remove MBs, and the supernatant is detected by fluorescence.The higher the concentration of acetamiprid, the greater the fluorescence intensity of supernatant, so as to realize the detection of acetamiprid.

The utility model relates to an acetamiprid efficacy detection device in the technical field of acetamiprid processing. The acetamiprid efficacy detection device comprises an operation table, and the upper end of the operation table is fixedly connected with a fixed frame; during use, the electric push rod needs to be started, the multiple second movable plates are pushed into the fixed frame, a sealing block is formed between every two adjacent second movable plates, the corresponding first movable plate and the corresponding second movable plate close to the first movable plate so that different kinds of pests can be contained, then the different kinds of pests and pesticide are placed in the different blocks, and after a period of time, the pesticide can be used for pesticide spraying. After detection is completed, an electric push rod is started, a second movable plate is pushed to continue to move, and when the second movable plate moves, the second movable plate drives a scraping plate to scrape off a pesticide and pest mixture attached to the operation table and push the pesticide and pest mixture out of a fixed frame, so that personnel can conveniently clean the operation table; and the pushed-out pesticide and pest mixture enters the waste box, so that people can conveniently collect the pesticide and pest mixture.

The invention discloses an iron-doped carbon dot nano-enzyme assisted ratio type electrochemical aptamer sensor, a preparation method and application, and belongs to the field of sensors. The method comprises the following steps: (1) preparing a molybdenum disulfide-gold nanoparticle-methylene blue composite material modified electrode; (2) preparing a MoS2 / Au / MB / cDNA modified electrode; (3) preparing the iron-doped carbon dot nano enzyme; (4) preparing the iron-doped carbon dots modified by the acetamiprid aptamer; and (5) preparing the MoS2 / Au / MB / cDNA (at) Apt / Fe-CDs modified electrode. The preparation method disclosed by the invention is low in cost, simple in experimental operation and easy in reaction condition control. The iron-doped carbon dot nano enzyme-assisted ratio-type electrochemical aptamer sensor prepared by the invention can be used as a working electrode for quantitative analysis and detection of acetamiprid, and has good stability, high sensitivity, high specificity and high accuracy in acetamiprid detection.

The present invention belongs to electrochemiluminescent aptamer sensor and relates to an electrochemical luminescent aptamer sensor based on Ag. + @Eu MOF / HOF resonance energy transfer electrochemiluminescence aptamer sensor and its application in acetamiprid detection. Using rod-shaped hydrogen bonded organic framework as precursor, doped with lanthanide element Eu 3+ Using Ag + As a co-reactant promoter, the synthesis of Ag + @Eu MOF / HOF was used as the energy donor of the resonance energy transfer system, and leaf-shaped CdS@Au-cDNA was used as the energy acceptor to prepare the electrochemiluminescent aptamer sensor for resonance energy transfer. The sensor was used for the detection of ACE, and it had a wide linear range and a low detection limit. + @Eu MOF / HOF electrochemiluminescent material was first used in electrochemiluminescence detection to detect the residues of acetamiprid in water, opening up an effective and convenient strategy for the analysis and detection of pollutants in water environments.

This invention provides a herbicidal composition comprising the active ingredients chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole, wherein the mass ratio of chlorpyrifos, oxazolidinyl acetamiprid, and isoxaflutole is 1~3:15~20:10~15. The herbicidal composition has a significant synergistic effect and can effectively solve the problem of weed resistance.

Pesticidal mixtures comprising as active compounds A) the ethylsulfone compound of formula (I) and B) at least one further compound B selected from fipronil, lambda-cyhalothrin, bifenthrin, tefluthrin, alpha- cypermethrin, thiamethoxam, clothianidin, acetamiprid, imidacloprid, dinotefuran, triflumezopyrim, fenmezoditiaz, spinosad, spinetoram, abamectin, afidopyropen, indoxacarb; metaflumizone, spiropidion, spidoxamat, chlorantraniliprole, cyantraniliprole, tetraniliprole, cyclaniliprole, broflanilide; dimpropyridaz, M.UN.1: 1-[6-(2,2- difluoro-7-methyl-[1,3]dioxolo[4,5-f]benzimidazol-6-yl)-5-ethylsulfonyl-3-pyridyl]cyclopropanecarbonitrile), M.UN.2: 6- (5-cyclopropyl-3-ethylsulfonyl-2-pyridyl)-2,2-difluoro-7-methyl-[1,3]dioxolo[4,5-f]benzimidazole); and difenoconazole, prothioconazole, flutriafol, ipconazole, tebuconazole, mefentrifluconazole, triticonazole, fluoxytioconazole, metyltetraprole, azoxystrobin, trifloxystrobin, pyraclostrobin, sedaxane, penflufen, fluopyram, fluxapyroxad, pydiflumetofen, isoflucypram, cyclobutrifluram, oxathiapiprolin, fluoxapriprolin, metalaxyl, picarbutrazox, fludioxonil, thiophanat-methyl; methods and use of these mixtures for combating invertebrate pests such as insects, arachnids or nematodes in and on plants, and for protecting such plants being infested with pests, especially also for protecting plant propagation material as like seeds.

The invention discloses a preparation method and application of an antigen for detecting impurities such as etoxazole in vegetables and fruits. The obtained antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting etoxazole contained in vegetables and fruits, the detection limit is 10 [mu] g / L. The detection result is high in accuracy, especially in an etoxazole antibody specificity experiment, obvious specificity to etoxazole is shown, and the detection result is not interfered by drugs such as carbendazim, triazophos, chlorpyrifos, cyfluthrin, metalaxyl, acetamiprid, difenoconazole, procymidone, chlorfenapyr, profenofos and omethoate.