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60 results about "Oxathiapiprolin" patented technology

Method for quickly identifying point mutation of nucleotide of phytophthora capsici leonian PcORP1 gene and pesticide resistance of mutant phytophthora capsici leonian PcORP1 gene to oxathiapiprolin

The invention relates to a method for identifying point mutation of nucleotide of a phytophthora capsici leonian oxysteroid binding protein related gene PcORP1 and an application of the mutant phytophthora capsici leonian PcORP1 gene to monitoring resistance to a bactericide oxathiapiprolin, and specifically discloses two molecular detecting methods and special primers for identifying drug resistance of mutation of 2379-site nucleotide of the phytophthora capsici leonian PcORP1 gene. The mutation site refers to G and T heterozygosis or T homozygosis of 2379-site nucleotide from 5' tail end of the phytophthora capsici leonian PcORP1 gene, so that the 769-site amino acid from the N-terminal of PcORP1 protein is heterozygous glycine and tryptophane or homozygous tryptophane. The molecular detecting method provided by the invention is high in sensitivity, good in stability and short in detecting period, can be used for monitoring resistance gene frequency as well as resistance occurence and development condition of field phytophthora capsici leonian to the bactericide oxathiapiprolin with high throughput, so that early warning of resistance to diseases is realized, the control strategy of the phytophthora capsici leonian is guided and timely regulated, the pesticide use is rational, the occurrence and the development of pesticide resistance are delayed, and an important role is played in a sustainable management system of diseases.
Owner:CHINA AGRI UNIV
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