The invention relates to a tolfenpyrad-containing pesticide composition for preventing and controlling nematodes, and an application thereof. The pesticide composition comprises an effective active component A and an effective active component B, the active ingredient A is tolfenpyrad, the active component B is selected from one of thiodicarb, monosultap, abamectin, fosthiazate, fluazaindolizine,fluensulfone, fluxapyroxad, trifluoro nematicide, Tioxazafen, phoxim, spirotetramat, dithiocyano-methane, berberine and carvacrol, and the weight ratio of the active component A to the active component B is 1:40 to 40:1. The pesticide composition disclosed by the invention has a relatively good prevention effect on crop root-knot nematodes, pine wood nematodes, cyst nematodes, stem nematodes and the like, and provides a preparation and an application mode which have a synergistic effect, are convenient to use, safe and efficient for preventing and controlling crop nematodes.