337 results about "Paecilomyces lilacinus" patented technology

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a compound bacterium for preventing and treating root-knot nematode and a preparation method thereof. The compound bacterium for preventing and treating root-knot nematode is prepared by the following steps: carrying out high-density cultivation on raw material strains of bacillus thuringiensis, pseudomonas fluorescens, bacillus subtilis, streptomycete, aspergillus niger, paecilomyces lilacinus and fusarium oxysporum, inoculating activated and cultivated strains in a fermentation tank and implementing fermentation and extended cultivation; dehydrating and drying various single strains obtained from extended cultivation to obtain resting microorganism dry powder; and mixing 10-20 parts of bacillus thuringiensis, 5-10 parts of pseudomonas fluorescens, 10-20 parts of bacillus subtilis, 10-20 parts of streptomycete, 10-15 parts of aspergillus niger, 10-25 parts of paecilomyces lilacinus and 8-15 parts of fusarium oxysporum to obtain the compound bacterium for preventing and treating root-knot nematode. The compound bacterium disclosed by the invention is used for preventing and treating root-knot nematode; and compared with an existing product, the compound bacterium is environment-friendly, harmless and non-toxic to a human body and good in control efficiency.

The invention provides a multifunctional active organic fertilizer and a preparation method thereof, relating to an organic fertilizer containing bacteria, fungi and spores, particularly an active organic fertilizer with functions of soil restoration and disease/pest resistance. The invention aims to provide a multifunctional active organic fertilizer capable of restoring soil pollution and improving product quality and a preparation method thereof. The multifunctional active organic fertilizer is composed of three layers from inside to outside, wherein the innermost layer is composed of a component A of which the main effective components are Bacillus subtilis and Paecilomyces lilacinus; the middle layer is composed of a component B and a component C containing multiple rich nutritive elements; and the outermost layer is composed of a component D of which the main effective components are Streptomyces vinaceus-drappus and Gliocladium roseum. The multifunctional active organic fertilizer can passivate heavy metals in soil, activate the soil, improve the microecological environment of the rhizosphere, degrade the chemical pesticides and improve the crop quality. The multifunctional active organic fertilizer is used in the field of agricultural planting.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to a nematoid-killing composition containing abamectin and paecilomyces lilacinus, which is characterized by comprising abamectin and paecilomyces lilacinus; 100g of nematoid-killing composition is prepared by the following components of 0.1-5g of abamectin, 0.1-1 billion live spores/g of paecilomyces lilacinus, 1-10g of stabilizing agent, 1-90g of solvent and 10-90g of assistant, wherein the assistant is any one or the combination of any two of attapulgite, kaoline, sepiolite, light calcium and kieselguhr and is prepared into a granular formulation or suspending agent or wettable powder; the stabilizing agent is any one or the combination of any two of castor oil, soybean oil, epoxidized soybean oil, propylene glycol, methyl oleate, ethylene glycol, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA); and the solvent is any one of dimethylbenzene, trimethylbenzene and dimethylformamide. The nematoid-killing composition is used for controlling the knot nematode and has better control effect on the knot nematode.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The invention discloses paecilomyces lilacinus and a cultivating method thereof and applications thereof. The method comprises steps of: A. activation of paecilomyces lilacinus PL461, which comprises steps of: placing paecilomyces lilacinus PL461 bacterial strains on a PDA medium board, cultivating the paecilomyces lilacinus PL461 bacterial strains, and activating the paecilomyces lilacinus PL461 bacterial strains; B. liquid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: putting the activated paecilomyces lilacinus PL461 bacterial strains into an Erlenmeyer flask containing a liquid medium of PDB and Czapek's, cultivating the above bacterial strains with shaking, and taking the above cultivated bacterial strains as standby; C. solid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: mixing a solid fermentation cultivation medium uniformly according to a volume ratio, wherein the medium comprises dry compost rice bran, corn flour and water, putting the mixed medium into a domestic fungus culture bag, inoculating the paecilomyces lilacinus PL461 fermented bacteria solution into the solid fermentation cultivation medium employing culture dish fermentation after disinfection and cooling, and putting the above inoculated solid fermentation cultivation medium into a culture dish after stirring uniformly again to cultivate and take as standby; and D. preparation of a paecilomyces lilacinus bacteria agent from the solid phase culture according to a formula and the method. The paecilomyces lilacinus has an obvious lethal effect on nematode eggs, equipment is simple, production cost is reduced greatly, and the cultivating method is suitable for mass production of paecilomyces lilacinus.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention discloses a new method for controlling vegetable root knot nematode, which is characterized in that one or two from chitin, aropaz, Paecilomyces lilacinus and nematicidal pioneer is/are selected to process a seedbed in 1 to 2 weeks before the field planting of vegetables; 1.0Kg to 2.0Kg /mu (1 acre equals to 6.07 mu) of Fosthiazate and 20Kg to 60Kg/mu of harmonia mundi are mixed to process soil before the field planting; after the field planting, a biological agent is matched and used; the method is convenient to be used, is safe and effective, not only can effectively control the root knot nematode, but also can promote the growing of the vegetable plants, has the double effects of pesticide and fertilizer and can lighten the environment pollution brought by the use of the pesticide in large dosage; in the early period, the numbers of the eelworms in the soil are reduced by the mechanical eelworm pesticide of Fosthiazate; the stress resistance of the vegetables are increased by the chitin and the pesticide-fertilizer harmonia mundi; during the later period of the field planting, the numbers of the eelworms remained in the soil are controlled by using any of the biological agent of chitin, the aropaz, the Paecilomyces lilacinus and the nematicidal pioneer to irrigate the root, thus achieving the effect of controlling the vegetable root knot nematode by the cooperation of biology and chemistry and having very high popularization value.

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

The invention discloses paecilomyces lilacinus and an application thereof. The provided paecilomyces lilacinus refers to paecilomyces lilacinus E16 with a register number of CGMCC No.5951 in the common microorganism center of the China microorganism culture preservation management committee. According to the paecilomyces lilacinus E16, in potting experiments, the control effect on second-instar banana root-knot nematodes is 86.37%, the growth rate to banana root weight is 37.76%, the growth rate to banana plant height is 56.47%, the growth rate to overground parts of banana is 38.03%; and in area experiments, the control effect on banana root-knot nematodes is 83.03% and the growth rate to banana fruit is 28.05%.

The invention provides a selenium-rich bio-organic fertilizer. The selenium-rich bio-organic fertilizer is prepared from the following ingredients in parts by mass: 40-80 parts of organic residue, 0.01-0.1 part of seleniferous compound, 10-15 parts of NPK, 0.5-1 part of microbial agent and 5-10 parts of auxiliaries, wherein the microbial agent is prepared from bacillus licheniformis, paecilomyces lilacinus and bacillus amyloliquefaciens, the effective viable count ratio of the bacillus licheniformis to the paecilomyces lilacinus to the bacillus amyloliquefaciens is (1 to 100): (1 to 200): (10 to 100), and the effective viable count of the microbial agent is greater than 0.2 billion per gram. According to the selenium-rich bio-organic fertilizer, selenium and the paecilomyces lilacinus are in synergism in a reasonable proportioning ratio, and thus the nematode inhibiting effect is better; and the seleniferous compound, organic matters, the microbial agent and the auxiliaries are in synergism, so as to improve the quality and yield of crops.

The invention discloses a facultative anaerobic fermentation fungicide. The facultative anaerobic fermentation fungicide is prepared from facultative anaerobic fermentation flora. The facultative anaerobic fermentation flora is prepared from lactic acid bacteria, lactobacillus acidophilus, bacillus subtilis, bacillus licheniformis, bacillus coagulans, aspergillus oryzae, bacillus megatherium, bacillus laterosporus, bacillus thuringiensis, streptococcus faecium, photosynthetic bacteria, saccharomycetes, paecilomyces lilacinus, nitrogen-fixing bacteria and actinomycetes. The facultative anaerobic fermentation fungicide can be suitable for facultative anaerobic fermentation of organic fertilizer, organic matter can be efficiently converted, and growth of pernicious bacteria can be inhibited. The invention also discloses a preparing method of the organic fertilizer, in the method, facultative anaerobic fermentation is carried out through the facultative anaerobic fermentation fungicide, the fermentation cycle is short, the running cost is low, the organic fertilizer prepared with the method is rich in nutriment, environmentally friendly and harmless, and development of ecological agriculture can be promoted.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion/g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

The invention relates to a microbial agent for promoting rootage, preventing continuous cropping and performing ecological restoration on soil for planting bananas and a preparation method thereof, and the microbial agent is prepared by inoculating a production strain into a sterile culture medium containing a carbon source, a nitrogen source, an inorganic salt and water, performing aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.4-7.6, performing primary amplification culture respectively, mixing primary amplification culture solutions with equal mass, further performing secondary and tertiary amplification culture, finally adsorbing fermentation end products and immobilizing on a carrier for preparing the microbial agent, wherein the production strain is selected from bacillus subtilis, bacillus licheniformis, saccharomyces bayanus, paecilomyces lilacinus, streptomyces microflavus and bacillus polymyxa. The microbial agent can be used for effectively preventing dead seedlings, yellow seedlings, wilt, leaf spots, root rot and other soil ecological diseases during the planting process of the bananas, the production cost is low, the fermentation period is short, the flora density is high, and the microbial agent can be used for further promoting the growth of roots, loosening the soil, strengthening the seedlings, removing the pollution caused by residues of pesticides and fertilizers and playing a soil ecological restoration role.

The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg/mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.

The invention provides a preparation method of composite biological organic fertilizer granules. The fertilizer granules comprise the following effective components: 50 to 55 parts of bacillus subtilis, 10 to 15 parts of actinomycetes, 8 to 10 parts of photosynthetic bacteria, 6 to 10 parts of paecilomyces lilacinus, 3 to 5 parts of saccharomycetes and 2 to 4 parts of bifidobacteria. The fertilizer granules which are prepared in a compositing manner have complete functions and high complementarity and can be mixed with chemical fertilizers and organic fertilizers, thereby improving the utilization rate of the fertilizer granules by 30% or so. The fertilizer granules are used for resisting diseases and inhibiting bacteria, improving soil fertility, activating soil, increasing yield and improving quality, resisting the lodging and decreasing pesticide residues, and are long in storage life, wherein the flower retention rate is improved by 50%; the fruit-bearing is advanced by 7 to 10 days; and the yield is increased by 15% to 20%. In addition, the fertilizer granules contain the white spines actinomycete streptomyces, thereby having obvious effects for inhibiting the pathogenic fungi of the fusarium wilt of cucumbers from growing. The experimental result proves that the morbidity of the fusarium wilt of the cucumbers is below 10% after the fertilizer granules provided by the invention are applied to the soil in which the fusarium wilt of the cucumbers occurs.

The invention relates to a paecilomyces lilacinus-containing composition for preventing and controlling nematodes. The composition contains paecilomyces lilacinus and acephate in a weight ratio of (50-600):(1-7), wherein the living bacterium content of the paecilomyces lilacinus is between 1 and 10 billion colony-forming units (cfu)/gram. The composition can prevent and control the nematodes effectively and exert a synergistic effect on the prevention and control over the nematodes. In addition, the acephate also can kill the nematodes, so pests in growth environment of crops also can be killed while an effect of killing the nematodes by the paecilomyces lilacinus is enhanced; and an effect of preventing and controlling the nematodes and other pests simultaneously by applying pesticides at one time is achieved.

The present invention discloses a strain of Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape. The Paecilomyces lilacinus of the present invention is Paecilomyces lilacinus YT08, has the preservation number of CGMCC No.10026, is preserved in the China General Microbiological Culture Collection Center (CGMCC) in November 26, 2014, provides strong prevention and control effects for meloidogyne of tomato and grape, and can be developed as the novel biological prevention and control agent for the agriculture meloidogyne diseases.