54 results about "Oxymatrine" patented technology

The invention discloses a special composition for resisting superficial fungal bacterial infection and a preparation method thereof. The composition mainly comprises an antibacterial agent, a synergist, a repairing agent and a solubilizer. The component of the antibacterial agent is epsilon-polylysine hydrochloride; the synergist is prepared from oxymatrine, hexanediol, caprylyl glycol and sodium diacetate; the repairing agent comprises the following components: glycerol, urea, allantoin and dipotassium glycyrrhizinate; the solubilizer is prepared from the following components: triton X-100, polyvinylpyrrolidone, sodium pyrrolidone carboxylate, sodium pyrithione and polyvinyl alcohol. According to the invention, food-grade and traditional Chinese medicine-derived antibacterial components are used, so that the cleaning agent is free of hormones and irritant substances, is non-corrosive and is friendly to human bodies. Meanwhile, the composition disclosed by the invention is in a water-soluble and spray form, is convenient to use, can soften cutin and dead skin, has good permeability, continuously plays a role in killing fungi and bacteria, and achieves the purpose of treating the root causes.

The invention relates to a temperature-sensitive gel nose drop and a preparation method and application thereof, and belongs to the technical field of biological medicines.The temperature-sensitive gel nose drop is composed of a temperature-sensitive gel matrix, active ingredients and pharmaceutic adjuvants, the temperature-sensitive gel matrix comprises poloxamer 407 and poloxamer 188, the active ingredients comprise matrine and oxymatrine, and the pharmaceutic adjuvants comprise the temperature-sensitive gel matrix, the active ingredients and the pharmaceutic adjuvants. The pharmaceutic adjuvants comprise one or more of a solvent, a humectant, an antioxidant, a preservative, a wetting agent, a thickening agent, a coloring agent, a wetting agent and a buffering agent. The temperature-sensitive gel nose drop disclosed by the invention is simple in preparation process, controllable in cost and suitable for large-scale production. The thermo-sensitive gel nose drop can improve the expression level of serum IgE, IL-4 and IFN-gamma, improves the symptom of allergic rhinitis by correcting Th1 / Th2 imbalance, is suitable for clinical treatment and prevention of allergic rhinitis, and has a remarkable clinical application prospect.

The invention belongs to the field of crop genetic breeding, and relates to cultivation of plants with high matrine and oxymatrine content. In particular discloses a transcription factor for positively regulating matrine and oxymatrine in plants, and a nucleotide sequence of the transcription factor has 90% similarity with a sequence as shown in SEQ ID No.1. Furthermore, the nucleotide sequence of the transcription factor is as shown in SEQ ID No. 1. By constructing an overexpression vector of a subprostrate sophora transcription factor (StNAC46 gene) and performing genetic transformation on the overexpression vector, it is proved that the StNAC46 gene can improve accumulation of matrine and oxymatrine in plants, and a new thought is provided for cultivation and production of subprostrate sophora with high matrine and oxymatrine content.

The invention relates to application of oxymatrine in preparation of a medicine for treating a patient suffering from erythroderma psoriasis and the like.

The invention provides a monascus strain ZX-99 capable of expressing multiple beneficial active substances and application of the monascus strain ZX-99, and belongs to the technical field of microorganisms. The preservation number of the monascus strain ZX-99 provided by the invention is CGMCC No.40962, the strain does not produce citrinin (the detection limit is 25 mg / kg), and compared with other monascus cheese and commercially available mould cheese, the prepared cheese sample has the advantages that the content of citrinin in the cheese sample is greatly reduced, and the content of citrinin in the cheese sample is greatly reduced. Substances such as oxymatrine, bufalin, azadirachtin, gamma-aminobutyric acid, hydroxybenzylamine, maleic acid, maltotriose, maltotetraose and diacetyl are enriched in the monascus cheese ZX-99, and the monascus cheese ZX-99 is expected to be used for developing biological functional dairy products or compounding flavor strains.

The invention relates to a preparation method of a nitraria tangutorum seed extract as well as a product and application of the nitraria tangutorum seed extract, the preparation method comprises the following steps: performing ultrasonic-assisted enzymolysis on crushed and sieved nitraria tangutorum seeds to obtain an enzymolysis product; carrying out subcritical extraction on the enzymolysis product by using subcritical water to obtain extract liquor; filtering and concentrating the extracting solution to obtain a nitraria meadowrue seed crude extract; and carrying out flocculation treatment and decoloration treatment on the nitraria meadowrue seed crude extract to obtain the nitraria meadowrue seed extract. According to the preparation method, ultrasonic-assisted enzymolysis and subcritical water extraction technologies are adopted, purification is further carried out through flocculation treatment and decoloration treatment, the nitraria sibirica seed extract rich in polysaccharide and oxymatrine can be obtained, no organic solvent or toxic and harmful chemical substances are used in the preparation process, and the preparation method is green, environmentally friendly and suitable for industrial production. The obtained nitraria seed extract is safe and non-irritant, has definite biological activity, can be applied to the field of skin care products, and realizes industrial production.

The present application relates to the application of traditional Chinese medicine active ingredient composition and its preparation in synergistic anti-tumor of PD-1 inhibitor. The traditional Chinese medicine active ingredient composition is a composition of oxymatrine and astragaloside B. The combination of the two can improve the anti-tumor effect of PD-1 inhibitor. In addition, it can also be used in the form of preparation to realize in vivo drug delivery in cooperation with its pharmaceutically acceptable carrier. The carrier can be selected from iron-based MOF and liposome, etc. to realize the co-loading of oxymatrine and astragaloside B with different polarity and efficient drug delivery. The iron-based MOF carrier has the advantages of high drug loading capacity and Fe ion which can cooperate with astragaloside B to improve the activity of tumor infiltrating T cells. The ferromagnetism and platelet membrane modification can also precisely target tumor tissue. The preparation technology of liposome is mature, which is conducive to industrialized production and has broad prospects for clinical transformation. The combination of the two with PD-1 inhibitor can significantly improve the anti-tumor effect of PD-1 inhibitor.

The invention relates to a pharmaceutical composition for treating hashimoto thyroiditis, and further relates to application of the pharmaceutical composition. The pharmaceutical composition comprises crocin and oxymatrine as active pharmaceutical ingredients, has an obvious improvement effect on serum TPO-Ab, TG-Ab, TSH, FT4, IFN-gamma, TNF-alpha and IL-10 levels of rats suffering from hashimoto thyroiditis, and is obviously superior to crocin and oxymatrine with the same dosage; the crocin and the oxymatrine have a synergistic effect in treatment of hashimoto thyroiditis, and the combination of the crocin and the oxymatrine has enhanced safety and wider application scenarios.

The invention discloses a novel application of oxymatrine in improving psoriasis symptoms by activating PPP2CA to inhibit ERalpha activity. The novel application comprises the following steps: animal experiments; cell experiments; determining the activity of the CCK-8 cell; carrying out Giemsa staining; the ELISA kit is used for detecting inflammatory cytokines IL-17 and IL-23; carrying out DIA proteomics; carrying out PPP2CA target verification; a PPP2CA siRNA knockout experiment is carried out; a PPP2CA overexpression plasmid experiment is carried out; evaluating the curative effect of the PPP2CA inhibitor; chemical proteomics; molecular docking; determining the enzyme activity of the PPP2CA; carrying out a thermal drift experiment (CETSA) / Western Blot experiment; phosphorylated proteomics; an estrogen signal channel key protein siRNA knock-down experiment; determining the ERalpha activity through a dual-luciferase reporter gene experiment; and statistical analysis. According to the invention, oxymatrine can be promoted to directly activate PPP2CA, so that phosphorylation in estrogen signal cascade is reduced, and the activity of an estrogen receptor alpha (ER alpha) is reduced.

The invention relates to a double-drug liposome co-carrying oxymatrine and an STING agonist prodrug as well as a preparation method and application of the double-drug liposome. The double-drug liposome comprises a drug carrier and oxymatrine, wherein the drug carrier consists of a membrane material; the oxymatrine is loaded in a hydrophilic inner cavity of the carrier; the membrane material is prepared from distearoyl phosphatidylcholine, distearoyl phosphatidyl ethanolamine-polyethylene glycol and a cholesterol-STING agonist prodrug conjugate; the cholesterol-STING agonist prodrug conjugate is a conjugate formed by coupling an STING agonist prodrug with cholesterol through a chemical bond. According to the invention, the traditional Chinese medicine active component oxymatrine and the STING agonist prodrug are creatively integrated into the same liposome, so that synergistic controlled release and mechanism complementation can be realized; wherein the STING prodrug is embedded into the carrier in a chemical bond coupling manner, so that drug leakage caused by physical entrapment is avoided, the acute toxicity of the free STING agonist is remarkably reduced, and meanwhile, the innate immune activation capability of the free STING agonist is enhanced.

The present invention discloses a multifunctional thermosensitive sustained-release hydrogel, its preparation method, and its use in the preparation of antipruritic and analgesic preparations. The present invention utilizes microspheres formed by a composite of hyaluronic acid and quaternary ammonium salt chitosan. These microspheres are sequentially mixed with a drug, a solubilizing agent-dissolved cooling substance, and poloxamer 407 using a simpler magnetic stirring method. This multifunctional thermosensitive sustained-release hydrogel is prepared without the use of a thickener. The hydrogel prepared by the present invention exhibits a strong cooling odor and sustained-release effect, along with good biocompatibility. When the drug used is an antipruritic and analgesic substance, such as oxymatrine, the hydrogel also has therapeutic and analgesic effects for non-histamine-dependent pruritus.

The application provides mefenamic acid-oxymatrine co-amorphous substance and a preparation method and application thereof, and belongs to the technical field of medicines. The mefenamic acid-oxymatrine co-amorphous substance is composed of mefenamic acid and oxymatrine with a molar ratio of 1:(0.5-3), and the co-amorphous substance is prepared by a grinding method or a solution crystallization method. The mefenamic acid-oxymatrine co-amorphous substance provided by the application has good stability, can significantly improve the solubility of mefenamic acid in water, thereby improving the bioavailability of mefenamic acid, and can also have synergistic effect.

The invention belongs to the technical field of soil conditioners, and particularly relates to a green and environment-friendly soil conditioner. The green and environment-friendly soil conditioner comprises a biopesticide component, the biopesticide component is formed by compounding paecilomyces lilacinus and pydiflumetofen or oxymatrine, and the spore content of the paecilomyces lilacinus is 20 billion live spores / g. The green and environment-friendly soil conditioner disclosed by the invention comprises the biopesticide component and the potassium humate, so that the green and environment-friendly soil conditioner not only can prevent and treat crop diseases, but also can condition soil and enhance the stress resistance of crops, and is beneficial to the growth of the crops and increase the yield of the crops.

The invention belongs to the technical field of biopesticides, and particularly relates to a biopesticide for preventing and treating tea lesser leafhopper, the active component of the biopesticide is formed by compounding metarhizium anisopliae and oxymatrine or tenvermectin; wherein the metarhizium anisopliae is a metarhizium anisopliae CQMa421 mother drug with a concentration of 15 billion spores per gram. According to the invention, the microbe source metarhizium anisopliae with insecticidal activity is reasonably compounded with other biopesticide components, and compared with the singly used source metarhizium anisopliae, the control effect on tea lesser leafhopper can be improved.

The application belongs to the technical field of biological medicine, and specifically discloses application of seco-oxymatrine in preparation of an antidepressant, wherein the antidepressant further contains a pharmaceutically acceptable carrier; the antidepressant is one of an oral preparation, an inhalant and an injection; the oral preparation is one of a suspension, an emulsion, a solution, a powder, a tablet, a granule and a capsule. When seco-oxymatrine is used as an effective component of the antidepressant, the depressive behavior of a LPS-induced depressive model mouse can be effectively improved.

The invention discloses a method for detecting effective components of a cervicitis treatment composition, which adopts high performance liquid chromatography for detection, and the chromatographic conditions are as follows: a chromatographic column is a C18 chromatographic column; the detection wavelength is 207 nm; a mobile phase A is a 0.2% phosphoric acid aqueous solution, a mobile phase B is acetonitrile, gradient elution is adopted, the mobile phase proportion is volume percent, and the elution procedure is as follows: 0-5 min, the mobile phase A is 100%, and the mobile phase B is 0%; the mobile phase A accounts for 100%-96% and the mobile phase B accounts for 0%-4% in 5-40 min; 40-60 min, the mobile phase A is 88%-58%, and the mobile phase B is 12%-42%; the mobile phase A accounts for 92% and the mobile phase B accounts for 8% in 60-80 min. The method disclosed by the invention can be used for simultaneously determining the components such as sophocarpidine, sophoridine, oxymatrine and amygdalin in the cervicitis composition, is convenient to operate, accurate in detection result and good in stability, and has very high practicability.

The invention provides a plant disease control composition, which comprises the following components: 8-25% of sophora alopecuroide total alkaloids, the sophora alopecuroide total alkaloids comprise sophocarpidine, oxymatrine and sophocarpidine, the mass ratio of the sophocarpidine to the oxymatrine to the sophocarpidine is (5-7): (2-3): (1-2); 10%-35% of tea tree oil; 3%-15% of a natural synergist; 5%-18% of a natural emulsifier; 12%-30% of a food-grade solvent; and the balance of deionized water. According to the composition for preventing and treating the plant diseases, the sophora alopecuroide total alkaloids and the high-purity tea tree oil in a specific proportion form a synergistic antibacterial-antiviral system, so that the composition generates synergistic control on fungal diseases and viral diseases, the limitation of narrow action spectrum of a single plant extract is broken through, and the CTC and passivation rate are remarkably increased by combining with a natural synergist; the invention further provides a method for preventing and treating plant diseases.

The invention belongs to the technical field of matrine preparation, and particularly relates to a method for converting sophocarpine in radix sophorae flavescentis percolate into matrine. Comprising the following steps: adding Na2SO3 into the radix sophorae flavescentis percolate, reacting at the temperature of 20-25 DEG C, namely near room temperature, and converting sophocarpine in the radix sophorae flavescentis percolate into sophocarpine; the mass volume ratio of the Na2SO3 to the radix sophorae flavescentis percolate solution is (0.022 g to 0.024 g): 1 mL. According to the method, efficient conversion of sophocarpine into sophocarpidine can be achieved by accurately controlling the adding amount of Na2SO3, the whole conversion process is mild, a catalyst and a high-temperature environment do not need to be added, and the efficiency of converting sophocarpidine into sophocarpidine is effectively improved.

The invention discloses matrine type alkaloid SA as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicine preparation. The matrine type alkaloid with a new structure is extracted from sophora moorcroftiana, and cell experiments find that the matrine type alkaloid not only has the effect of resisting hepatitis B virus (HBV), but also has the inhibitory effect on hepatitis B surface antigen (HBsAg) superior to that of matrine and oxymatrine of the same kind, and also superior to that of lamivudine. Therefore, the matrine type alkaloid can be used for preparing the medicine for treating or preventing hepatitis B virus (HBV) infection.

The invention belongs to the technical field of natural pharmaceutical chemistry, and particularly relates to a method for extracting chemical components in vietnamese sophora roots and a method for measuring the content of the chemical components in the vietnamese sophora roots. The preparation method comprises the following steps: mixing subprostrate sophora powder and a deep-eutectic solvent, soaking, and performing ultrasonic extraction to obtain a subprostrate sophora powder extracting solution; the radix sophorae tonkinensis powder extracting solution contains at least one of oxymatrine, oxysophocarpine, maackiain and matrine; the eutectic solvent comprises a hydrogen bond donor and a hydrogen bond acceptor; the hydrogen bond donor is ethylene glycol; the hydrogen bond receptor is betaine. The extraction efficiency of the four target compounds in the vietnamese sophora root can be remarkably improved by adopting the deep-eutectic solvent assisted by ultrasonic extraction.

The invention discloses application of oroxin B in preparation of a medicine for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, hydrogel of the oroxin B and a preparation method of the hydrogel, and belongs to the technical field of biomedical materials. The invention firstly provides application of oroxin B in preparation of a medicine for promoting osteogenic differentiation of bone marrow mesenchymal stem cells. The invention also provides a self-assembled polypeptide hydrogel loaded with the oroxin B. The hydrogel is obtained by combining the oroxin B with the self-assembled polypeptide with a specific sequence (SEQ ID No.1) and carrying out mild physical blending and incubation. The hydrogel forms a three-dimensional nanofiber network, can entrap and continuously release oroxin B in situ, not only can effectively collect bone marrow mesenchymal stem cells, but also can provide an ideal microenvironment for proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells, and remarkably up-regulate osteogenic related gene expression, so that bone defect repair is expected to be accelerated.

The invention provides application of oxymatrine in preparation of a medicine for inhibiting jellyfish toxin, and belongs to the technical field of biological medicine. Screening tests are carried out on multiple drugs, and it is found that the oxymatrine OMT has a better antagonistic effect on TE toxin than other drugs, and cytotoxicity is not exposed. Further research finds that oxymatrine can reduce H9C2 cell apoptosis, antagonize cell apoptosis and oxidative damage caused by the ormopilema esculentum toxin, increase the survival rate of mice injected with the ormopilema esculentum toxin and improve mouse heart tissue damage caused by the ormopilema esculentum toxin, has huge potential in preparation of TE antagonistic drugs, and can be used for preparing TE antagonistic drugs. The medicine can be used as a candidate medicine for treating the stings of the sandfish.

The invention provides a strain of rhizopus arrhizus JHK31, a fungicide containing the rhizopus arrhizus JHK31 and application of the rhizopus arrhizus JHK31 and the fungicide in sophora flavescens fermentation. The rhizopus arrhizus JHK31 and the fungicide thereof can convert oxymatrine into matrine through fermentation, so that the yield of matrine is increased, the production cost of matrine is reduced, the problem of high production cost of matrine in the prior art is solved, the production cost of matrine biopesticide is indirectly reduced, and the application prospect is wide. And the matrine can be widely applied and popularized in sophora flavescens fermentation.

The invention belongs to the technical field of hydrogel and fruit preservation, and relates to glycyrrhizic acid / oxymatrine / berberine co-assembled hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving glycyrrhizic acid in water, continuously stirring at 70-90 DEG C until the glycyrrhizic acid is completely dissolved to obtain a glycyrrhizic acid solution, adding oxymatrine, then adding berberine, uniformly stirring, and then cooling to room temperature to form glycyrrhizic acid / oxymatrine / berberine co-assembled hydrogel; the molar ratio of oxymatrine to glycyrrhizic acid is below 1: 2, and the molar ratio of glycyrrhizic acid to berberine is 12: (0.25-6.0). According to the invention, glycyrrhizic acid, oxymatrine and berberine are taken as raw materials, and are co-assembled to form the hydrogel; the fruit preservative is sprayed before picking, is easy to adhere to and accumulate on the surfaces of fruits, enhances the fresh-keeping and antibacterial capabilities, improves the fresh-keeping effect of the fruits, and reduces the risk of physical damage caused by post-picking treatment at the same time.

The application provides a flufenamic acid-oxymatrine co-amorphous substance and a preparation method and application thereof, and belongs to the technical field of medicines.The flufenamic acid-oxymatrine co-amorphous substance is composed of flufenamic acid and oxymatrine in a molar ratio of 3:1-1:3, and the co-amorphous substance is prepared by a grinding method or a solution crystallization method.The flufenamic acid-oxymatrine co-amorphous substance has good stability, can significantly improve the solubility of flufenamic acid in water, thereby improving the bioavailability of flufenamic acid, and can also have synergistic effects.

This invention provides the application of oxymatrine in the preparation of drugs that inhibit jellyfish toxins, belonging to the field of biomedical technology. Screening experiments were conducted on various drugs, revealing that oxymatrine (OMT) was more effective than other drugs in antagonizing jellyfish toxins without exhibiting cytotoxicity. Further research showed that oxymatrine can reduce H9C2 cell apoptosis, antagonize apoptosis and oxidative damage induced by jellyfish toxins, improve the survival rate of mice after injection of jellyfish toxins, and improve cardiac tissue damage caused by jellyfish toxins in mice. It shows great potential in the preparation of jellyfish toxin antagonistic drugs and can serve as a candidate drug for treating jellyfish stings.

The invention provides a flufenamic acid-aloperine co-amorphous substance as well as a preparation method and application thereof, and belongs to the technical field of medicines. The flufenamic acid-aloperine co-amorphous substance is prepared from flufenamic acid and aloperine in a molar ratio of (3: 1)-(1: 3), and the co-amorphous substance is prepared by a grinding method or a solution crystallization method. The flufenamic acid-aloperine co-amorphous substance provided by the invention has good stability, and the solubility of flufenamic acid in water can be remarkably improved, so that the bioavailability of the flufenamic acid is improved, and the flufenamic acid-aloperine co-amorphous substance can achieve synergistic interaction.

The invention belongs to the technical field of biomedical materials. The invention provides a preparation method of an ultrasonic piezoelectric composite scaffold for promoting bone repair, which comprises the following steps: mixing oxymatrine and saikoside C as a mixed medicine; the mixed medicine and a stent obtained by sintering hollow barium titanate nanoparticles and hydroxyapatite powder and performing high-pressure polarization treatment are jointly placed in water, after adsorption equilibrium is achieved through stirring, the free mixed medicine is removed, and the ultrasonic piezoelectric composite stent for promoting bone repair is obtained. Wherein the weight ratio of the oxymatrine to the saikoside C is (3-5): 1, and the weight ratio of the hollow barium titanate nanoparticles to the hydroxyapatite powder is 1: 1. According to the invention, the effect of the stent material and the carried medicine on promoting bone repair is fully exerted, the synergistic effect of the material and the carried medicine on promoting osteogenic differentiation is realized, and the stent has a good application prospect.

The application discloses a quality control method of radix stephaniae tetrandrae decoction pieces, adopts liquid chromatography-mass spectrometry to detect the efficacy components in the radix stephaniae tetrandrae decoction pieces, and the efficacy components include any one or several of tetrandrine, fangchinoline, cycilanol, stepholidine, oxystepholidine, stephaoxime, corynoxeine, miliradin and oxymatrine. The specific detection condition can detect multiple efficacy components in the radix stephaniae tetrandrae decoction pieces, which is helpful for comprehensively controlling the quality of the radix stephaniae tetrandrae decoction pieces, and finding the possibility of replacing wild radix stephaniae tetrandrae through efficacy evaluation. In addition, the thin layer chromatography is used to identify the radix stephaniae tetrandrae decoction pieces before detection, and the identification sensitivity can be improved by adjusting the thin layer chromatography condition.

The invention discloses multifunctional temperature-sensitive slow-release hydrogel, a preparation method thereof and application of the multifunctional temperature-sensitive slow-release hydrogel in preparation of an itching-relieving and pain-easing preparation. The preparation method comprises the following steps: sequentially mixing microspheres formed by compounding hyaluronic acid and quaternary ammonium salt chitosan with a medicine, a substance which is dissolved by a solubilizer and has a cool smell and poloxamer 407 by adopting a simpler magnetic stirring method, and preparing the multifunctional temperature-sensitive slow-release hydrogel on the premise of not using a thickening agent. The hydrogel prepared by the preparation method disclosed by the invention has relatively strong cool smell and slow release effect, and has good biocompatibility; when the used medicine is a substance with the itching-relieving and pain-relieving effect, such as oxymatrine, the hydrogel also has the effects of treating non-histamine-dependent pruritus and easing pain.