118 results about "Naringenin" patented technology

The invention belongs to the technical field of biology, discloses application of naringenin in resisting siniperca chuatsi and frog iridovirus, and discovers the antiviral activity of the naringenin to the siniperca chuatsi and frog iridovirus, the naringenin can down-regulate the expression level of NF-kB and MAPK pathway genes triggered by MRV, effectively inhibit MRV-induced excessive inflammatory response and inhibit MRV proliferation, and the application of the naringenin in resisting the siniperca chuatsi and frog iridovirus. In addition, tissue damage caused by MRV infection can be relieved, and the survival rate of MRV infected fishes is effectively increased; a foundation is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus by adopting naringenin; meanwhile, the invention also discloses a drug containing naringenin, a fish feed and a fish feed additive.

The invention discloses radix tetrastigme flavone synthase and application thereof. The radix tetrastigme flavone synthase comprises an amino acid sequence as shown in SEQ ID No. 2. The key enzyme-flavone synthase gene (ThFNS) related to synthesis of apigenin in radix tetrastigme is cloned for the first time, in-vitro recombinant protein expression and enzymatic reaction system verification show that flavone synthase can catalyze naringenin to generate apigenin, and technical support is provided for follow-up metabolic engineering modification for an apigenin biosynthetic pathway.

The invention discloses a gingko GbDRF2 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbDFR2 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbDFR2 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbDFR2 gene is shown as SEQ ID NO.2. A GbDFR2 overexpression vector is constructed and nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the contents of 9 flavonoid substances such as sakuranetin and 3, 7-dimethyl quercetin are increased, and the contents of 10 metabolites such as naringin and chalcone are decreased. The invention discloses a regulation effect of the ginkgo GbDFR2 gene in synthesis of flavonoid compounds, and provides a theoretical basis and a molecular tool for improving plant secondary metabolites through genetic engineering.

The present application relates to the field of microbiology and biotechnology, in particular, the present application relates to an engineered microorganism, a culture comprising the same, a pharmaceutical composition and a dietary supplement, and their use in the manufacture of a medicament. The present application also provides a method for culturing the engineered microorganism and a method for producing naringenin.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention belongs to the field of polymer adhesive materials, and particularly relates to a naringenin modified lignin phenolic resin adhesive and a preparation method thereof. The naringenin modified lignin phenolic resin adhesive is prepared from the following raw materials in parts by mass: 60 to 70 parts of formaldehyde solution, 65 to 85 parts of phenol, 6 to 14 parts of naringenin, 10 to 20 parts of lignin, 12 to 18 parts of alkali metal hydroxide and 25 to 30 parts of water. The mass concentration of the formaldehyde solution is 35%-40%. The phenolic resin adhesive is prepared by taking the biological phenol naringenin and the lignin as raw materials to replace part of phenol, so that the aim of environmental protection is fulfilled by replacing non-renewable resources with renewable resources, the bonding strength and the thermal performance of phenolic resin are improved, and the application field of the biological phenolic resin adhesive is widened.

The invention provides a compound skin care micro-formula based on exocarpium citri grandis and application thereof, and belongs to the field of skin care products. The composition comprises a pomelo fruit extract, a notopterygium root extract, an African chandelia fruit extract, a crambe abyssinica leaf extract and a delivery substance. According to the invention, four bioactive pathways are integrated to form a cascade repair network; and a traditional plant compounding framework is broken through, and two types of novel functional components are introduced. Meanwhile, aiming at the transdermal problem of fat-soluble components such as naringin, a micro-emulsion delivery system is developed, and the particle size is controlled: the micro-emulsion system is not layered for 6 months in a 40 DEG C / RH 75% acceleration test. The composition has both independent use and compounding compatibility, and can meet diversified market requirements.

The invention belongs to the technical field of analysis and detection, and particularly relates to a method for detecting 2-hydroxynaringenin in plants. The detection method comprises the following steps: extracting a plant sample to obtain a liquid to be detected; detecting the liquid to be detected by adopting high performance liquid chromatography-tandem mass spectrometry to obtain the peak area of the 2-hydroxynaringenin in the plant sample; and according to the peak area of the 2-hydroxynaringenin and a preset standard curve, calculating the content of the 2-hydroxynaringenin in the plant sample, the detection conditions of the ultra-high performance liquid chromatography are as follows: a mobile phase A is water; the mobile phase B is acetonitrile; the ion source temperature of the ultra-high performance liquid chromatography is 500 DEG C. The method has the advantages of favorable linear relationship (favorable linear relationship (r gt; 0.999)), favorable precision and favorable repeatability, and can accurately determine the content of 2-hydroxynaringenin in plants.

The invention relates to application of naringenin in treatment of non-renaturable pulpitis.

The invention provides high-naringenin-content modified shaddock peel fiber gel and a preparation method thereof.The preparation method comprises the following steps that after shaddock peel white pulp is subjected to quick freezing and smashing, pretreatment is conducted through an electrochemistry-assisted gradient enzymolysis technology, then customized immobilized naringinase is adopted for treatment, and high-naringenin-content shaddock peel fibers are obtained; and finally, uniformly mixing the fiber with carnosic acid and soybean saponin, and adding magnesium chloride for cross-linking and curing to obtain the modified shaddock peel fiber gel. According to the preparation method, electrochemical assisted gradient enzymolysis is adopted, so that a compact network structure of fibers can be destroyed to increase active sites, the immobilized naringinase not only can efficiently convert naringin and accurately enrich naringenin, but also can effectively improve bitter taste, and magnesium ions regulate and control a multiple cross-linked network formed by the components, so that the gel structure tends to be more stable; meanwhile, naringenin is stably loaded so as to avoid migration and aggregation of the naringenin, and the bioavailability and active efficacy of the naringenin are remarkably improved.

The application belongs to the field of food processing and biomedical technology, and discloses a preparation method of a salty taste enhancing peptide-naringenin compound and application of the compound in improving cognitive decline. The compound is prepared by compounding salty taste enhancing peptides from soft-shelled turtle eggs and naringenin at a ratio of 20-150:1 (w:w). The preparation process comprises the following steps: soft-shelled turtle egg enzymolysis, ultrafiltration, freeze-drying to obtain salty taste enhancing peptides, mixing the salty taste enhancing peptides with a naringenin solution, concentration, and freeze-drying. The compound has good stability, salty taste enhancing and antioxidant activities, can reduce the use of salt and maintain salty taste. The compound can improve cognitive decline and anxiety caused by aging and diabetes, improve autonomous activity, memory and social skills, strengthen physical fitness, protect nerve cells and skeletal muscles, maintain brain tissue health, and can be applied to the preparation of related preparations for improving cognitive decline.

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.

The present invention provides a sweetener composition with improved taste quality of a sweet substance. The present invention relates to a sweetener composition containing the following components (A), (B), and (C): (A) at least one γ-glutamyl peptide selected from the group consisting of a compound represented by the formula (I): γ-Glu-X-Gly (I) wherein X is an amino acid residue or an amino acid derivative residue, and a compound represented by the formula (II): γ-Glu-Y (II) wherein Y is an amino acid residue or an amino acid derivative residue, or a salt thereof, (B) at least one compound selected from the group consisting of naringenin, hesperetin, and pinocembrin, or a salt thereof, and (C) a sweet substance.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention belongs to the technical field of naringenin producing enzyme modification. The invention provides an At4CL3 enzyme mutant. The amino acid sequence of the At4CL3 enzyme mutant is shown in SEQ ID NO. 2 in a sequence table. The At4CL3 enzyme mutant provided by the invention can be used for effectively improving the yield of naringenin.

The present application relates to the technical field of microbial pesticides, in particular to a deep-sea streptomyces with antifeedant function and antibacterial effect and application thereof. The streptomyces from the South China Sea deep sea is Streptomyces sp. NA13, which was preserved in China Center for Type Culture Collection on August 29, 2022, and the preservation number is CCTCC M 20221343. The strain has the ability to prepare antibiotics such as Antimycin, Candicidin, Naringenin, Surugamide, Fredericamycin, SAL-2242, Albaflavenone, Hopene, SGR PTMs, etc. In particular, the fermentation is used to prepare antimycin or cyclic octapeptide surugamide compounds, including novel antimycin compounds antimycin Q (1). The prepared antimycin compounds are used as insect pest antifeedants or antifungal drug lead compounds, and have application prospects of developing into natural source antibacterial and insecticidal pesticides.

The invention relates to the technical field of preparation of veterinary antiviral traditional Chinese medicines, in particular to application of naringenin in preparation of an anti-EHV-8 medicine, including application of a naringenin monomer, a plant extract containing the naringenin monomer and a traditional Chinese medicine compound in preparation of an EHV-8 replication inhibiting medicine. The naringenin disclosed by the invention plays an antiviral role in an EHV-8 replication stage, cannot directly inactivate viruses, has the maximum safe concentration of 100 mu M, has a remarkable inhibition effect on a plurality of strains, can reduce lung injury of a mouse model caused by EHV-8 infection, is natural in source and low in toxicity, has various pharmacological activities such as anti-inflammation and the like, and provides a reference for anti-EHV-8 clinical medication.

The present invention belongs to the field of functional foods, and specifically relates to a composition for lowering glucose and stabilizing an insulin level, a preparation method therefor, and use thereof. The composition of the present invention comprises a component I and a component II. The component I is cyclodextrin and a derivative thereof, the component II is naringenin and a derivative thereof, and the mass ratio of the component I to the component II is (0.1-100):(0.01-1). After being eaten with a meal or added to food, especially high-carbohydrate food, the composition of the present invention can significantly maintain the stability of the insulin level in the body while significantly controlling the postprandial blood glucose level. Long-term use can achieve the effects of stabilizing triglyceride, low-density lipoprotein, and high-density lipoprotein levels.

The invention belongs to the technical field of circuit board repair, and particularly relates to a PCB copper surface repair process. The process comprises the following steps: detecting the copper surface of the PCB, identifying and positioning defects, and generating a three-dimensional damage map; a cleaning solution containing tripotassium citrate, sodium lauroyl sarcosinate, propyl gallate, glycerin and Tr ici ne is used for conducting fixed-point cleaning on the defect area; spraying a functional nano activator dispersion liquid prepared from a layered zirconium oxide nanosheet, carboxymethyl chitosan, ammonium tripolyphosphate, a naringenin-dopamine-maleic acid copolymer and a polyvinyl alcohol aqueous solution to form a nano-structure activation layer; and finally, applying a conductive material for defect filling and repairing. According to the process, the copper surface micro-defects can be accurately repaired, the conductivity and the interface bonding force of the repaired area are enhanced, the repaired PCB has excellent long-term stability, the overall reliability of the PCB is effectively improved, and the service life of the PCB is effectively prolonged.

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.

The invention discloses a hemolysin-like protease inhibition composition for regulating vaginal micro-ecological balance as well as a preparation method and application of the hemolysin-like protease inhibition composition. The hemolysin-like protease inhibition composition disclosed by the invention is prepared from 10 to 20 percent of lactobacillus fermentation liquor, 10 to 30 percent of phloretin, 10 to 30 percent of naringenin, 30 to 40 percent of quercetin and 5 to 10 percent of sodium hyaluronate. The preparation method comprises the following steps: dissolving phloretin, naringenin, quercetin and sodium hyaluronate in 50% butanediol in proportion, and ultrasonically mixing uniformly to obtain a mixed solution; and slowly adding the mixed solution into the lactic acid bacteria fermentation solution, and uniformly stirring to obtain the hemolysin-like protease inhibition composition. According to the invention, natural active components with a hemolysin-like protease inhibition effect are screened to construct a synergistic system with three mechanisms of acidification environment regulation, enzyme activity inhibition and barrier repair, so that hemolysin-like protease can be directly inhibited, and the effect of inhibiting the hemolysin-like protease can be achieved by adjusting the local pH value and the micro-ecological environment of the vagina. Propagation of pathogenic bacteria is inhibited, and growth of dominant flora such as lactobacillus is promoted.

The application discloses a CRP mutant gene and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the CRP mutant gene is shown in SEQ ID NO. 7 or SEQ ID NO. 8 or SEQ ID NO. 9, the CRP mutant gene can significantly eliminate the 'glucose inhibition' effect, activate glycolysis and TCA cycle in a basic medium with glucose as a carbon source, strengthen the utilization and biosynthesis of glucose, and promote the growth of a strain, and the CRP mutant gene with the amino acid sequence shown in SEQ ID NO. 7, 8 or 9 also has the effect of improving the expression amount of vanillin or naringenin of the strain.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The application belongs to the technical field of circuit board repair, and particularly relates to a PCB copper surface repair process. The process comprises the following steps: detecting the copper surface of a PCB, identifying and positioning defects, and generating a three-dimensional damage atlas; using a cleaning solution containing tri-potassium citrate, sodium lauroyl sarcosinate, propyl gallate, glycerol and Trici ne to perform spot cleaning on the defect area; spraying a functional nano-activating agent dispersion liquid made of layered zirconium oxide nanosheets, carboxymethyl chitosan, ammonium tripolyphosphate, naringenin-dopamine-maleic acid copolymer and polyvinyl alcohol aqueous solution to form a nano-structure activation layer; and finally applying a conductive material to fill and repair the defects. The process can realize accurate repair of copper surface micro-defects, enhance the conductivity and interface bonding force of the repaired area, and the repaired PCB has excellent long-term stability, effectively improving the overall reliability and service life of the PCB.

The invention discloses a method for preparing hollow nanoparticles from soybean protein isolate-wheat gliadin compound protein and delivering naringenin, and belongs to the technical field of food application. The method comprises the following steps: extracting soybean protein isolate, preparing a soybean protein isolate ethanol solution, preparing a wheat gliadin ethanol solution, carrying out alkaline heating, preparing hollow nanoparticles by taking sodium carbonate as a hard template, preparing a naringenin ethanol stock solution, and preparing the naringenin-loaded hollow nanoparticles. The functional characteristics of the soybean protein isolate and the wheat gliadin are improved, the requirements of the green food industry for environment-friendly and natural auxiliary materials are closely combined, a new path is provided for widening the application of plant protein in the field of green functional ingredients, and the method is simple to operate, low in cost and suitable for industrial production. And the food industry can be promoted to be transformed to a more nutritional, safer and more sustainable direction. The method for preparing the hollow nano-particle loaded naringenin by combining alkali heat treatment with an anti-solvent precipitation method lays a foundation for application of the hollow nano-particles in the food field and research in biological medicine.

The invention discloses an injectable bone repair material and a preparation method thereof, and belongs to the technical field of biological materials. The material is characterized in that N-formylglycine, allyl lactate and S-allylcysteine react and then react with chitosan to obtain grafted chitosan, the grafted chitosan, methacrylated gelatin and icariin-naringenin lipidosome are formed, and the stable three-dimensional interpenetrating network structure hydrogel is formed through photocuring. The preparation method comprises the following steps: firstly synthesizing grafted chitosan, then preparing icariin-naringenin liposome, finally mixing a grafted chitosan solution, a methacrylated gelatin solution and liposome, adding a photoinitiator, and carrying out ultrasonic degassing and ultraviolet curing to obtain a finished product. The material utilizes thioether bonds to realize active oxygen responsive drug intelligent release, liposome encapsulation improves the drug utilization rate, a double-network structure endows the material with excellent injectability, mechanical strength and biocompatibility, and the material can effectively adapt to irregular bone defects and promote bone regeneration.

Disclosed is an aptamer capable of binding to luteolin and other naringenin derived flavonoids, wherein the aptamer is: an RNA aptamer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2; or a DNA aptamer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NO: 4; wherein for SEQ ID NO: 1 and 2, N is one nucleotide selected from the group consisting of A, G, C and U, and wherein for SEQ ID NO: 3 and 4, N is one nucleotide selected from the group consisting of A, G, C and T. Also disclosed is a riboswitch and a DNA expression cassette comprising the aptamer, a composition thereof, and a microbe containing the riboswitch or DNA expression cassette. Further disclosed is a method of detecting luteolin and other naringenin derived flavonoids produced in a microbe using the aptamer or riboswitch as disclosed herein.

The invention discloses bio-based waterborne polyurethane as well as a preparation method and application thereof. The bio-based waterborne polyurethane is prepared from the following raw materials in parts by weight: 25-50 parts of a bio-based polyurethane prepolymer, 1-2 parts of a chain extender, 0.1-0.5 part of a cross-linking agent, 0.3-1.5 parts of an end-capping reagent and 0.5-2 parts of a neutralizer. The bio-based polyurethane prepolymer is a bio-based polyurethane prepolymer modified by a natural polyphenol compound. The natural polyphenol compound naringenin is used as a rigid ultraviolet absorption and crosslinking unit, gallic acid is used as a strong hydrogen bond construction unit, and the units are chemically bonded into a polyurethane main chain, so that the prepared waterborne polyurethane has excellent mechanical properties and full-wave-band ultraviolet shielding capability; and the prepared bio-based waterborne polyurethane emulsion has excellent stability and long-acting corrosion resistance, and is suitable for the fields of high-end corrosion prevention, outdoor weather resistance, flexible protective coatings and the like.

The present invention relates to the combination of guava leaf extract, naringenin and an enzyme selected from lysozyme, labiase or a combination thereof for use in a dental or oral care product.

The invention relates to the technical field of biology, in particular to a preparation method of naringenin-7, 4 '-di-O-beta-D-glucopyranoside and application of the naringenin-7, 4'-di-O-beta-D-glucopyranoside in cosmetics, the structural formula of the naringenin-7, 4 '-di-O-beta-D-glucopyranoside is as follows: # imgabs0, and the naringenin derivative is prepared by taking naringenin as a raw material. The obtained product is high in purity and yield, meanwhile, the effect of the product is not transmitted to naringenin, and the product has excellent water solubility, bioavailability and cell affinity.