163 results about "Naringenin" patented technology

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention discloses a Sarcabomyces lipolytica strain for synthesizing (2S)-naringenin and application of the Sarcabomyces lipolytica strain, and belongs to the technical field of engineering and bioengineering. According to the invention, a key enzyme CHS is subjected to semi-rational transformation, so that the yield of the strain (2S)-naringenin expressing the mutant V196A / T132C is increased by 60%. According to the method, the modified CHS is further integrated into a yeast genome, conversion of p-coumaric acid to (2S)-naringenin is promoted by strengthening a shikimic acid pathway and enhancing supply of cofactors, so that the yield of the (2S)-naringenin reaches 776mg / L after the constructed strain is fermented in a 24-pore plate for 96 hours, and the yield of the constructed strain can reach 8.65 g / L after the strain is fermented in a 5L fermentation tank; the method lays a foundation for the subsequent biosynthesis of flavonoid compounds, and has potential value and significance for the development of synthetic biology.

The invention discloses an application of naringenin nanoparticles in preparation of a ceftiofur long-acting preparation. The invention discloses naringenin (5, 7-dihydroxy-2 (4-hydroxyphenyl) chroman-4-ketone), which is a flavonoid substance. The naringenin nanoparticles are prepared, the naringenin nanoparticles are mixed with the ceftiofur for combined use, and it is found that the naringenin can effectively intervene in the pharmacokinetic process of the ceftiofur and significantly prolong the in-vivo half-life period of the ceftiofur, so that the in-vivo action time of the ceftiofur is prolonged, and the curative effect of the ceftiofur is improved. Moreover, the application method is simple, easy to operate, low in cost and suitable for large-scale popularization.

The invention relates to the technical field of bioengineering, and discloses a genetically engineered bacterium and a method for producing icaritin through whole-cell catalysis. By constructing double plasmids, choline kinase, isopentenyl phosphokinase, flavonoid 3-hydroxylase, flavonol synthase, isopentenyl transferase and oxymethyltransferase are simultaneously overexpressed in escherichia coli, so that a genetically engineered bacterium is constructed, and the engineering bacterium is used as a whole-cell catalyst to prepare a whole-cell engineering bacterium. The naringenin and the 3-methyl-2-butene-1-ol are used as substrates to be converted into the icaritin in a whole-cell catalysis mode, only one-step reaction is needed, the method is simple, rapid and high in efficiency, other chemical substances do not need to be additionally added in the conversion process, the cost is reduced, pollution to the environment is relieved, and the method is suitable for industrial production. And the biological production from low-price naringenin to high-added-value icaritin is realized.

The invention provides a high internal phase emulsion as well as preparation and application thereof. The preparation method comprises the following steps: firstly, carrying out hydration, uniform mixing and enzymolysis on walnut protein and protein A, then uniformly mixing a double-protein zymolyte with naringenin dispersed in a dispersing agent, carrying out ultrasonic treatment, adding into water, finally uniformly mixing with coconut oil, and homogenizing to obtain the high internal phase emulsion. According to the present invention, the specific oil-water interface stabilizer naringenin is added to the double-protein zymolyte system, such that the walnut protein can maintain the good solubility and the good emulsifying property even in the pH 5-7 environment so as to significantly improve the physical stability of the high internal phase emulsion, and the daily chemical product prepared from the high internal phase emulsion has the good moisturizing effect, the application of the walnut protein in the field of daily chemical products is widened.

The invention belongs to the technical field of medicines, and particularly relates to a flavonoid compound-meglumine co-amorphous substance and a preparation method thereof. The flavonoid compounds comprise baicalein, hesperetin, naringenin, kaempferol, quercetin, isoliquiritigenin and the like, and the co-amorphous substance consists of a single flavonoid compound and meglumine and is prepared by adopting a melting shock cooling method or a rotary evaporation method. The miscibility of the flavonoid compounds and the meglumine is screened through Hansen solubility. The crystal diffraction characteristics, thermodynamic properties and intermolecular interaction of the co-amorphous substance are measured through PXRD, DSC and FTIR, and it is shown that the six flavonoid compounds and meglumine successfully form a co-amorphous system. The solubility and the dissolution rate of the six flavonoid compound-meglumine co-amorphous substance are obviously superior to those of flavonoid bulk drugs, and the physical stability of the formed co-amorphous substance is obviously superior to that of a single amorphous drug, so that the co-amorphous substance shows good patent medicine performance.

The invention belongs to the technical field of biology, discloses application of naringenin in resisting siniperca chuatsi and frog iridovirus, and discovers the antiviral activity of the naringenin to the siniperca chuatsi and frog iridovirus, the naringenin can down-regulate the expression level of NF-kB and MAPK pathway genes triggered by MRV, effectively inhibit MRV-induced excessive inflammatory response and inhibit MRV proliferation, and the application of the naringenin in resisting the siniperca chuatsi and frog iridovirus. In addition, tissue damage caused by MRV infection can be relieved, and the survival rate of MRV infected fishes is effectively increased; a foundation is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus by adopting naringenin; meanwhile, the invention also discloses a drug containing naringenin, a fish feed and a fish feed additive.

The invention discloses radix tetrastigme flavone synthase and application thereof. The radix tetrastigme flavone synthase comprises an amino acid sequence as shown in SEQ ID No. 2. The key enzyme-flavone synthase gene (ThFNS) related to synthesis of apigenin in radix tetrastigme is cloned for the first time, in-vitro recombinant protein expression and enzymatic reaction system verification show that flavone synthase can catalyze naringenin to generate apigenin, and technical support is provided for follow-up metabolic engineering modification for an apigenin biosynthetic pathway.

The invention discloses a Polygonatum cyrtonema PcF3H (Polygonatum cyrtonema PcF3H) gene. The invention also discloses a method for promoting the synthesis of the flavanone compound. The invention also discloses an application of the Polygonatum cyrtonema PcF3H gene in promoting the synthesis of flavanone compounds. The invention also discloses an application of the Polygonatum cyrtonema PcF3H gene in catalysis of naringenin hydroxylation. The polygonatum cyrtonema PcF3H gene is obtained through cloning, a plant expression vector is constructed, the polygonatum cyrtonema PcF3H gene is instantaneously transformed into tobacco, and compared with a control, the content of flavanone compounds in tobacco leaves over-expressed with the polygonatum cyrtonema PcF3H gene is remarkably increased. An applicant utilizes a prokaryotic expression system to induce and purify a protein product PcF3H protein expressing the polygonatum cyrtonema PcF3H gene, the PcF3H protein takes naringenin as a substrate, and a result shows that the product PcF3H protein of the polygonatum cyrtonema PcF3H gene has a function of catalyzing naringenin hydroxylation reaction.

The invention relates to the technical field of crop planting, and particularly discloses application and method of Naringenin in improving the flooding stress resistance of tomatoes. Naringenin is sprayed to leaves of tomato plants in the seedling stage and irrigated to roots of the tomato plants, so that the flooding resistance of the tomatoes can be improved, and Naringenin can be used as a regulator for tomato seedlings to resist a flooding environment. According to the method, 0.25 mM to 1.00 mM of naringenin solution is applied to tomato plants, so that lodging, leaf dryness and seedling leaf withering of tomato seedlings are relieved, and the flooding stress resistance of tomatoes is remarkably improved.

The invention discloses a gingko GbDRF2 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbDFR2 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbDFR2 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbDFR2 gene is shown as SEQ ID NO.2. A GbDFR2 overexpression vector is constructed and nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the contents of 9 flavonoid substances such as sakuranetin and 3, 7-dimethyl quercetin are increased, and the contents of 10 metabolites such as naringin and chalcone are decreased. The invention discloses a regulation effect of the ginkgo GbDFR2 gene in synthesis of flavonoid compounds, and provides a theoretical basis and a molecular tool for improving plant secondary metabolites through genetic engineering.

The present application relates to the field of microbiology and biotechnology, in particular, the present application relates to an engineered microorganism, a culture comprising the same, a pharmaceutical composition and a dietary supplement, and their use in the manufacture of a medicament. The present application also provides a method for culturing the engineered microorganism and a method for producing naringenin.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention belongs to the field of polymer adhesive materials, and particularly relates to a naringenin modified lignin phenolic resin adhesive and a preparation method thereof. The naringenin modified lignin phenolic resin adhesive is prepared from the following raw materials in parts by mass: 60 to 70 parts of formaldehyde solution, 65 to 85 parts of phenol, 6 to 14 parts of naringenin, 10 to 20 parts of lignin, 12 to 18 parts of alkali metal hydroxide and 25 to 30 parts of water. The mass concentration of the formaldehyde solution is 35%-40%. The phenolic resin adhesive is prepared by taking the biological phenol naringenin and the lignin as raw materials to replace part of phenol, so that the aim of environmental protection is fulfilled by replacing non-renewable resources with renewable resources, the bonding strength and the thermal performance of phenolic resin are improved, and the application field of the biological phenolic resin adhesive is widened.

The present invention discloses a L-glutamine-zein-naringenin nanoparticle and its application, which is a nanoparticle formed by zein self-assembly encapsulating naringenin, indicating that electrostatic adsorption has chitosan oligosaccharide grafted with L-glutamine. The present invention is prepared by natural and non-toxic materials such as naringenin, zein, chitosan oligosaccharide, etc. The results show that the nanoparticle has been successfully synthesized, and at the same time, the drug encapsulation rate is high and the expectation of no toxicity to cells is achieved. Through in vitro cell experiments, it is confirmed that it has antioxidant activity, has pH responsiveness so that it can be specifically selected to be released in large quantities in the small intestine, and in addition, it can also improve cell insulin resistance and fat metabolism. The present invention uses nanoparticles constructed from natural plant materials, and the system is safe and non-toxic, can play the function of targeted intestinal transportation of hydrophobic active substances, improve drug absorption and utilization, and then produce therapeutic effects.

The invention provides a compound skin care micro-formula based on exocarpium citri grandis and application thereof, and belongs to the field of skin care products. The composition comprises a pomelo fruit extract, a notopterygium root extract, an African chandelia fruit extract, a crambe abyssinica leaf extract and a delivery substance. According to the invention, four bioactive pathways are integrated to form a cascade repair network; and a traditional plant compounding framework is broken through, and two types of novel functional components are introduced. Meanwhile, aiming at the transdermal problem of fat-soluble components such as naringin, a micro-emulsion delivery system is developed, and the particle size is controlled: the micro-emulsion system is not layered for 6 months in a 40 DEG C / RH 75% acceleration test. The composition has both independent use and compounding compatibility, and can meet diversified market requirements.

The invention belongs to the technical field of analysis and detection, and particularly relates to a method for detecting 2-hydroxynaringenin in plants. The detection method comprises the following steps: extracting a plant sample to obtain a liquid to be detected; detecting the liquid to be detected by adopting high performance liquid chromatography-tandem mass spectrometry to obtain the peak area of the 2-hydroxynaringenin in the plant sample; and according to the peak area of the 2-hydroxynaringenin and a preset standard curve, calculating the content of the 2-hydroxynaringenin in the plant sample, the detection conditions of the ultra-high performance liquid chromatography are as follows: a mobile phase A is water; the mobile phase B is acetonitrile; the ion source temperature of the ultra-high performance liquid chromatography is 500 DEG C. The method has the advantages of favorable linear relationship (favorable linear relationship (r gt; 0.999)), favorable precision and favorable repeatability, and can accurately determine the content of 2-hydroxynaringenin in plants.

The invention relates to application of naringenin in treatment of non-renaturable pulpitis.

The invention provides high-naringenin-content modified shaddock peel fiber gel and a preparation method thereof.The preparation method comprises the following steps that after shaddock peel white pulp is subjected to quick freezing and smashing, pretreatment is conducted through an electrochemistry-assisted gradient enzymolysis technology, then customized immobilized naringinase is adopted for treatment, and high-naringenin-content shaddock peel fibers are obtained; and finally, uniformly mixing the fiber with carnosic acid and soybean saponin, and adding magnesium chloride for cross-linking and curing to obtain the modified shaddock peel fiber gel. According to the preparation method, electrochemical assisted gradient enzymolysis is adopted, so that a compact network structure of fibers can be destroyed to increase active sites, the immobilized naringinase not only can efficiently convert naringin and accurately enrich naringenin, but also can effectively improve bitter taste, and magnesium ions regulate and control a multiple cross-linked network formed by the components, so that the gel structure tends to be more stable; meanwhile, naringenin is stably loaded so as to avoid migration and aggregation of the naringenin, and the bioavailability and active efficacy of the naringenin are remarkably improved.

The application belongs to the field of food processing and biomedical technology, and discloses a preparation method of a salty taste enhancing peptide-naringenin compound and application of the compound in improving cognitive decline. The compound is prepared by compounding salty taste enhancing peptides from soft-shelled turtle eggs and naringenin at a ratio of 20-150:1 (w:w). The preparation process comprises the following steps: soft-shelled turtle egg enzymolysis, ultrafiltration, freeze-drying to obtain salty taste enhancing peptides, mixing the salty taste enhancing peptides with a naringenin solution, concentration, and freeze-drying. The compound has good stability, salty taste enhancing and antioxidant activities, can reduce the use of salt and maintain salty taste. The compound can improve cognitive decline and anxiety caused by aging and diabetes, improve autonomous activity, memory and social skills, strengthen physical fitness, protect nerve cells and skeletal muscles, maintain brain tissue health, and can be applied to the preparation of related preparations for improving cognitive decline.

The application discloses a naringenin-antibacterial peptide composite liposome hydrogel and a preparation method and application thereof, and relates to the technical field of medicine, in particular to a naringenin-antibacterial peptide composite liposome hydrogel, which is prepared by the following steps: dissolving soybean lecithin, cholesterol and naringenin in a chloroform-methanol mixed solvent, rotary evaporation under reduced pressure, and vacuum solvent removal to obtain a naringenin-loaded lipid film; dissolving a bacillus subtilis antibacterial peptide in a PBS buffer solution to prepare a hydration solution, adding the lipid film to the hydration solution to rotate and hydrate to obtain a crude liposome suspension, and extruding the liposome suspension through polycarbonate membranes at room temperature to obtain a co-loaded liposome suspension; dispersing carbomer 940 in pure water, standing and swelling to prepare a gel matrix, mixing a humectant and a preservative into the gel matrix, and adding a neutralizing agent dropwise to adjust the pH value to 6.8-7.0; and mixing the liposome suspension and the gel matrix at room temperature, adding a PBS buffer solution to the mixture to prepare the naringenin-bacillus subtilis antibacterial peptide co-loaded liposome hydrogel, so as to solve the problems of skin irritation and bacterial drug resistance caused by long-term use of traditional acne treatment drugs.

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.

The present invention provides a sweetener composition with improved taste quality of a sweet substance. The present invention relates to a sweetener composition containing the following components (A), (B), and (C): (A) at least one γ-glutamyl peptide selected from the group consisting of a compound represented by the formula (I): γ-Glu-X-Gly (I) wherein X is an amino acid residue or an amino acid derivative residue, and a compound represented by the formula (II): γ-Glu-Y (II) wherein Y is an amino acid residue or an amino acid derivative residue, or a salt thereof, (B) at least one compound selected from the group consisting of naringenin, hesperetin, and pinocembrin, or a salt thereof, and (C) a sweet substance.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention belongs to the technical field of naringenin producing enzyme modification. The invention provides an At4CL3 enzyme mutant. The amino acid sequence of the At4CL3 enzyme mutant is shown in SEQ ID NO. 2 in a sequence table. The At4CL3 enzyme mutant provided by the invention can be used for effectively improving the yield of naringenin.

The present application relates to the technical field of microbial pesticides, in particular to a deep-sea streptomyces with antifeedant function and antibacterial effect and application thereof. The streptomyces from the South China Sea deep sea is Streptomyces sp. NA13, which was preserved in China Center for Type Culture Collection on August 29, 2022, and the preservation number is CCTCC M 20221343. The strain has the ability to prepare antibiotics such as Antimycin, Candicidin, Naringenin, Surugamide, Fredericamycin, SAL-2242, Albaflavenone, Hopene, SGR PTMs, etc. In particular, the fermentation is used to prepare antimycin or cyclic octapeptide surugamide compounds, including novel antimycin compounds antimycin Q (1). The prepared antimycin compounds are used as insect pest antifeedants or antifungal drug lead compounds, and have application prospects of developing into natural source antibacterial and insecticidal pesticides.

The invention relates to the technical field of preparation of veterinary antiviral traditional Chinese medicines, in particular to application of naringenin in preparation of an anti-EHV-8 medicine, including application of a naringenin monomer, a plant extract containing the naringenin monomer and a traditional Chinese medicine compound in preparation of an EHV-8 replication inhibiting medicine. The naringenin disclosed by the invention plays an antiviral role in an EHV-8 replication stage, cannot directly inactivate viruses, has the maximum safe concentration of 100 mu M, has a remarkable inhibition effect on a plurality of strains, can reduce lung injury of a mouse model caused by EHV-8 infection, is natural in source and low in toxicity, has various pharmacological activities such as anti-inflammation and the like, and provides a reference for anti-EHV-8 clinical medication.

The present invention belongs to the field of functional foods, and specifically relates to a composition for lowering glucose and stabilizing an insulin level, a preparation method therefor, and use thereof. The composition of the present invention comprises a component I and a component II. The component I is cyclodextrin and a derivative thereof, the component II is naringenin and a derivative thereof, and the mass ratio of the component I to the component II is (0.1-100):(0.01-1). After being eaten with a meal or added to food, especially high-carbohydrate food, the composition of the present invention can significantly maintain the stability of the insulin level in the body while significantly controlling the postprandial blood glucose level. Long-term use can achieve the effects of stabilizing triglyceride, low-density lipoprotein, and high-density lipoprotein levels.

The invention belongs to the technical field of circuit board repair, and particularly relates to a PCB copper surface repair process. The process comprises the following steps: detecting the copper surface of the PCB, identifying and positioning defects, and generating a three-dimensional damage map; a cleaning solution containing tripotassium citrate, sodium lauroyl sarcosinate, propyl gallate, glycerin and Tr ici ne is used for conducting fixed-point cleaning on the defect area; spraying a functional nano activator dispersion liquid prepared from a layered zirconium oxide nanosheet, carboxymethyl chitosan, ammonium tripolyphosphate, a naringenin-dopamine-maleic acid copolymer and a polyvinyl alcohol aqueous solution to form a nano-structure activation layer; and finally, applying a conductive material for defect filling and repairing. According to the process, the copper surface micro-defects can be accurately repaired, the conductivity and the interface bonding force of the repaired area are enhanced, the repaired PCB has excellent long-term stability, the overall reliability of the PCB is effectively improved, and the service life of the PCB is effectively prolonged.

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.