295 results about "Naringenin" patented technology

Formulations and methods of treatment and putative prevention for aging (anti-aging composition) and for diseases or conditions of all reactive oxygen species-dependant illnesses, such as Alzheimer's disease, Parkinson's disease, diabetes mellitus, cardiovascular disease, cancer, hepatitis, and disorders associated with estrogen deficiencies including osteoporosis and breast cancer and for improving athletic performance of humans include resveratrol and two (2) or more of the following features or additional active ingredients: (1) slow release formulation of resveratrol; (2) pterostilbene; (3) quercetin; (4) fisetin, and (5) naringenin. Slow release is defined for the purposes of the present invention as releasing 95% of the active agent or agents in eight (8) hours through normal human gastrointestinal absorption.

An application of naringenin and its salt in preparing the medicines for treating cough and resolving phlegm is disclosed. Said naringenin is prepared from naringin through hydrolysis or from the natural medicinal materials through extracting, or by chemical synthesis. Its advantages are high curative effect and no toxic by-effect.

In the invented method, citrus bioflavonoid and corresponding enzyme are used as raw materials, being dissolved in organic solvent, adding glucoside solution at concentration of 1-100g/L, under pH value of 3.0-7.0 and temperature of 20-65 deg.C for 20-60 min. After separation and purification, obtained is citrus bioflavonoid monoglycoside. With this invention, naringoside enzymolysis and cirmtim enzymolysis method for production of naringen in monoglycoside and hesperetin monoglycoside, compared with chemical method, has advantages of:easily to be controlled of enzymolysis, mild reaction, less by-products and easy to be separated of products. By using this invented method, the enzymolysis of naringoside and cirmtim for production of naringenin monocoside and hesperetin monoglycoside can be effectively controlled, and only the first glycosidic bond is broken, so obtained is highest yield of naringenin monoglycoside, hesperetin monoglycoside and rhamnose.

The invention discloses an anti-bacterial high-viscosity medical glue, which is prepared from the following ingredients: 2-cyano-acrylate isopropyl, polyisoprene, polypropylene, povidone iodine, magnesium sulfate, naringenin, halloysitum rubrum nano-powder, vitamin A palmitate, plant essential oil, paraffins, alginate, disodium EDTA, methacrylamide, polyvinyl alcohol, dioctyl phthalate, 1.5g/ml luffa extract, 0.8g/ml rhizoma bletillae extract, glycerol and sterile water. The invention further discloses a preparation method of the anti-bacterial high-viscosity medical glue. The adhesive strength and 48-hour compressive strength of the medical glue are in a relatively high level, the medical glue is high in anti-bacterial ratio, short in hemostasis time, high in overall healing efficiency, free from irritation on wound tissues and excellent in overall performance, and conforms to the application requirements of clinics for the medical glue.

The invention provides a preparation method of naringenin. The preparation method comprises the following steps: (1) preparing solution of naringin in lower alcohol or lower ketone solvent, leading the naringin in the prepared solution to be subjected to acid hydrolysis in an acidic condition to obtain naringenin, distilling under reduced pressure to remove solvent to obtain a naringenin crude product; (2) dissolving the naringenin crude product in absolute ethanol or absolute methanol, adding active carbon, stirring to decolor; and (3) filtering the active carbon, separating and purifying the prepared solution to obtain the naringenin. The preparation method has the advantages of few reaction steps, less used solvent, less emission of the 'three wastes', mild reaction condition and complete reaction and easy operation, is suitable for industrial production, and has higher practical value; and the prepared product has more stable quality, the purity is higher than 99 percent, which is higher than the drug standard.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The present invention discloses a quality control method for effective parts of pomelo flavedo and a pomelo flavedo preparation. According to the present invention, a methanol solution containing neoeriocitrin, prunin, naringin, rhoifolin, meranzin hydrate, 7-(2''-alpha-rhamnose-6''-(3''''-hydroxy-3''-methylglutaryl)-beta-D-glucosyl)naringenin, and naringenin is used as a reference; a methanol extract of pomelo flavedo effective parts or the pomelo flavedo preparation is used as a test sample; in particular chromatographic conditions, the high performance liquid chromatographic (HPLC) fingerprint analysis method is used to establish fingerprints and control fingerprints of a reference HPLC and a test sample HPLC, whether a drug is qualified is determined by comparing peaks of the test sample with corresponding peaks of the reference, and similarity evaluation results with the control fingerprint; and contents of various active ingredients in the test sample are simultaneously determined by a method of multi-component quantitive by one marker, and the quality of the drug is evaluated according to the contents of the active ingredients. The method can achieve scientific, comprehensive, rapid and accurate monitoring of the quality of effective parts and the preparation in production. The production process stability is controlled, the quality is ensured to be stable, uniform and controllable, and the method has the advantages of being advanced, good stability and reproducibility, and high operability.

The invention discloses a genetic engineering stain taking tyrosine as a substrate to synthesize naringenin and a construction method thereof, belonging to the field of synthetic biology or metabolic engineering. According to the construction method disclosed by the invention, a synthesis route constituted by six genes, namely a gene matB encoding a malonic acid transporting enzyme, a gene matC encoding a malonic acid absorption route, a gene encoding a tyrosine ammonialyase (TAL), the gene encoding a 4-cinnamic acid: coenzyme A ligase (4CL), the gene encoding a chalcone synthase (CHS) and the gene encoding a chalcone isomerase (CHI) is introduced into Escherichia coli BL21 to obtain a recombinant strain capable of taking the tyrosine as the substrate to synthesize the naringenin, the synthesis route is further optimized through a modular transformation theory, the strain is finally fermented in a shaking flask for 72 hours by taking the tyrosine as the substrate, and the yield of the naringenin achieves 90mg/L. A strategy adopted by the construction method disclosed by the invention provides certain reference significance for future production of natural small molecular substances by a microbiological method.

The use of naringenin and a salt thereof to enhance the sweetness of a sweetness modifier and to decrease the amount of a sweetness modifier used in a consumable is provided.

The invention provides a method for enzymatically synthesizing astragalin. The method comprises the following steps that 1, key enzymes needed in the synthesizing process of astragalin are cloned, expressed and purified, wherein the key enzymes comprise glycogen phosphorylase GP, glucose pyrophosphorylase GalU, flavanone-3-hydroxylase F3H, flavanone synthase FLS1 and flavonoid3-O-glucanotransferase UGT78K2; 2, glycogen Gn is synthesized into glucose-1-phosphoric acid G-1-P under the GP effect; 3, G-1-P is synthesized into uridine diphosphate UDPG under the GalU effect; 4, naringenin NRN is synthesized into dihydro kaempferol DHK under the F3H effect; 5, DHK is synthesized into kaempferol KMF under the FLS1 effect; 6, KMF and UDPG are synthesized into astragalin under the UGT78K2 effect. According to the method, few steps are adopted, the operation condition is mild, few side products are generated, the yield is large, pollution is avoided, and the production cost is remarkably reduced.

The invention discloses the application of naringenin and aurantiin as the inhibitor of the signal passage of transforming growth factor-Beta1, in particular the application to the treatment or prevention of fibrosis and tumors.

The invention relates to detection of flavonoids in tobacco, in particular to a metabonomics method for detecting multiple flavonoids in fresh tobacco leaves.The method is characterized in that flavonoids in the tobacco leaves are extracted through an ultrasonic extraction method, and rutin, quercetin, naringenin, kaempferol glucoside, astragalin, naringin and isoliquiritigenin in the fresh tobacco leaves are detected through UPLC-QqQ-MS/MS.Compared with the prior art, the method has the advantages that accuracy and sensitivity are high, repeatability is good, and the method is suitable for simultaneously and quantitatively detecting the seven flavonoid compounds in plants and can be used for research on metabolic pathways and gene functions of plant flavonoid matter.

The invention relates to a method for extracting naringenin from grapefruits. The method comprises the following steps: taking dry falling grapefruits, carrying out water-extraction, filtering by using an ultrafiltration membrane, carrying out reduced pressure concentration and crystallization, carrying out acid hydrolysis, and crystallizing the obtained product, so that an over 98% naringenin product is obtained. The method is simple and easy to operate, easily available in raw materials, short in time consuming, less in energy consumption, high in extraction ratio, unnecessary for column passing, and suitable for industrial mass production, less organic solvent is used, the cost is saved, and the environmental pollution is reduced.

The invention discloses a method for preparing scutellarin and analogues thereof. The method comprises the following steps of: preparing a 7-OH receptor compound from naringenin; preparing a uronic acid donor compound from glucuronic acid 3,6 lactone; and reacting the 7-OH receptor compound with the uronic acid donor compound to obtain scutellarin or analogues thereof finally. In the method, naringenin is taken as a raw material, and is cheap and readily available; a reaction route is flexible, and a series of analogues of scutellarin are convenient to obtain; and the obtained analogues of scutellarin have higher bioavailability.

The invention discloses a preparation method of a naringenin molecular imprinting electrochemical sensor. The preparation method is characterized in that firstly, a glassy carbon electrode is modified with a nano silver/carbon nanotube, and a nano silver/carbon nanotube modified electrode is obtained; 50%-60% of ethanol, 15%-20% of dipentaerythritol triacrylate, 4%-8% of methacrylic acid, 12%-18% of 1-vinyl-3-ethyl imidazole tetrafluoroborate, 1.0%-2.0% of azo-bis-iso-heptonitrile and 3.0%-6.0% of naringenin are added to a reactor, stirred, dissolved and stirred to react at the temperature of 65+/-2 DEG C for 12-15 h in an oxygen-free atmosphere, template molecules are removed through a methanol and acetic acid mixed solution, and naringenin molecular imprinting polymers are obtained; the naringenin molecular imprinting polymers are dispensed to the nano silver/carbon nanotube modified electrode, and the naringenin molecular imprinting electrochemical sensor is obtained. The sensor has high affinity, selectivity and sensitivity and good specificity, can realize rapid detection and can be repeatedly used.

The invention relates to an electronic cigarette liquid. The embodiment of the invention provides the electronic tobacco liquid. The electronic tobacco liquid comprises naringenin, wherein the contentof the naringenin in the electronic cigarette liquid is 0.09%-10% of the total weight of the electronic cigarette liquid. The electronic cigarette liquid is atomized, wherein the naringenin containedin the electronic cigarette liquid can be atomized together with the tobacco liquid and absorbed by a human body, so that the effects of diminishing inflammation, relieving cough and eliminating phlegm, inhibiting oral cavity and respiratory tract bacteria, and enabling the electronic cigarette liquid to be more comfortable in taste after being atomized can be achieved, the fragrance of the cigarettes is relatively mild, the fragrance of the cigarettes is enriched, and the smoking quality is improved.

The invention relates to a method for preparing solid naringenin and particularly relates to a method for preparing naringenin ultrafine particle by using a supercritical compressed fluid anti-solvent precipitation process, so as to produce the naringenin ultrafine particle with an improved dissolution rate. By regulating pressure, temperature, solution concentration, CO2 flow rate and the like, the particle size of the naringenin ultrafine particle can be controlled effectively; the particle size of the prepared naringenin ultrafine particle is in a range of 0.5mu m to 6mu m and the product yield is above 85%. The accumulative release rate of the prepared naringenin ultrafine particle within 240min is increased by 3.2 times in comparison with that of a naringenin raw material.

The invention belongs to the field of traditional Chinese medicine product extraction and separation, particularly relates to an extraction method and application of a red peony synergy whitening extractive, and is used for solving the problems of high content of impurities including pigment and the like and poor whitening effect since the traditional whitening cosmetics in which traditional Chinese medicine extractives are added are generally prepared from traditional Chinese medicine crude extractives. The extraction method of the red peony synergy whitening extractive comprises the following steps: dipping, and carrying out alcohol extraction, concentration, centrifugation and purification to obtain the product. The product contains seven peony monoterpenoid glycoside compounds and four flavonoid compounds, wherein the seven peony monoterpenoid glycoside compounds consist of paeoniflorin sulfurous ester, oxypaeoniflorin, albiflorin std, paeoniflorin, galloyl paeoniflorin, benzoyloxy paeoniflorin and benzoylpaeoniflorin, and the four flavonoid compounds consist of catechinic acid, dihydroquercetin, astragalin and naringenin. The product has a tyrosinase inhibition function, a blood activation function and an oxidation resistance function, and possesses the characteristics of traditional Chinese medicine cosmetic which has a diversity of functional components performing a synergy whitening function.

The present invention relates to delivery systems to enhance the bioavailability of flavonoids to improve human health. Flavonoids of interest include but are not limited to curcumin, resveratrol, ellagic acid, naringenin, and quercetin. Flavonoids are important in part because they are known to have beneficial effects on human health, including cardioprotective, antioxidant, and anticancer effects. Utility has been limited by low bioavailability, both in the sense of requiring high doses and in that it has been difficult to carry out proper dose-response studies in the absence of methods to control the actual dose administered. In addition to pharmaceutical applications, there are potential nutraceutical uses, for example in supplements that might be sold in health food stores and pharmacies.

The invention discloses purpose of naringenin, naringenin nanoliposome and a preparation method and application thereof. The naringenin is applied in the preparation of drugs for treating non-alcoholic fatty liver. The naringenin nanoliposome comprises naringenin and nanoliposome. The nanoliposome comprises phosphatide and cholesterol. The mass ratio of naringenin to phosphatide to cholesterol is 1:4-9:1-2. The preparation method comprises the following steps: 1) dissolving naringenin, phosphatide and cholesterol in a solvent, mixing, and removing the solvent to obtain a mixture; 2) hydrating the mixture obtained in the step 1) by the use of an aqueous medium to obtain a naringenin nanoliposome crude suspension; and 3) successively carrying out water-bath ultrasonic treatment and probe ultrasonic treatment on the naringenin nanoliposome crude suspension obtained in the step 2), so as to obtain the naringenin nanoliposome. The naringenin nanoliposome can raise oral bioavailability of naringenin and enhance the control effect of naringenin on non-alcoholic fatty liver.

The present invention discloses a blood sugar reducing composition and an application thereof, and belongs to the technical field of medicines. The blood sugar reducing composition is prepared from 3-O-methyl quercetin and naringenin according to a mass concentration ratio of (1:25) to (6:25). Preferably, the mass concentration ratio of the 3-O-methyl quercetin to the naringenin is (2:25) to (6:25). The 3-O-methyl quercetin and naringenin composition has the obvious alpha-glucosidase inhibiting synergistic effect, the effect is superior to that of a flavone compound used alone, the medicine dosage can be reduced, and the occurrence of drug resistance is reduced; and the composition has the wide application prospect in the preparation of diabetes treatment medicines, health care products orfoods.

The invention discloses a wikstroemia indica (L.) C.A.Mey extract as well as a preparation method and application thereof. The wikstroemia indica (L.) C.A.Mey extract has the effective compositions of: naringenin, 5, 6, 7- trihydroxy-4'-methoxyflavanone, p-hydroxybenzoic acid, benzoic acid, quercetin glycoside,genkwano1 B, daphnoretin, Lirioresinol, Sikokianin and the like. The invention simultaneously provides application of the wikstroemia indica (L.) C.A.Mey extract, in particular the favorable application in preparing anti-cancer or anti-inflammation analgesia drug. A cytotoxicity experiment shows that the wikstroemia indica (L.) C.A.Mey extract disclosed by the invention has cytotoxicity functions on breast cancer of human, breast cancer drugresistant cell strain, mouse breast adenocarcinoma, which are in linear relation.

The invention belongs to the field of a medical technology and specifically relates to a co-crystal formed by naringenin and isonicotinamide, wherein stoichiometric ratio of naringenin to isonicotinamide in the co-crystal is 1:2. The co-crystal has an X-ray powder diffraction pattern, a differential scanning calorimetry pattern and an infrared spectrogram which are different from those of naringenin crystals and a physical mixture of naringenin and isonicotinamide, is a novel crystalline form completely different from naringenin, and has remarkably-enhanced solubility.

The invention relates to a hypoglycemic compound, its preparation method and a health food with hypoglycemic function. The compound contains the following components of: by weight, 300-600 parts of propolis, 300-600 parts of naringenin, 60-120 parts of anthocyanidin, 0.5-1.5 part of momordica polypeptide, 0.001-0.01 part of vitamin D3, 0.001-0.1 part of vitamin H, 8-15 parts of vitamin E, 0.01-0.2 part of chromium element and 1-20 parts of zinc element. The hypoglycemic compound can be prepared by directly mixing raw materials containing the above components. According to the invention, with the combination of the above components, a good synergistic effect is generated among all the components. The invention has advantages of obvious hypoglycemic function, easily-obtained raw materials, simple preparation and relatively low cost. In addition, the components of the hypoglycemic compound are all natural bioactive substances. Therefore, the compound has good security and no toxic and side-effect and is especially applicable to be used as a health food for adjunctive treatment of diabetic patients.

The invention provides a salmon and trout feed based on a peroxisome proliferator activated receptor (PPAR) and a method for preparing the salmon and trout feed. The salmon and trout feed comprises proteins, fat, trace elements and other basic substances which are required by the salmon and trout growth, and further contains natural PPAR alpha and/or PPAR beta binding active substances, such as daidzin, curcumin, isoflavone, arachidonic acid, berberine, naringenin, polydatin, kaempferol, isoflavoues aglycone, conjugated linoleic acid and the like. The salmon and trout feed can regulate the fat and fatty acid anabolism of the cultured salmon and trout, the fat content of the salmon and trout is lowered, the beneficial omega-3 fatty acid content is improved, and the salmon and trout quality is improved.

The invention discloses a semi compounding method for apium celery element that uses natural extract as basic raw material. It uses pomelo peel element as raw material, 1, 4-dioxy cyclohexane as reaction solvent, taking oxidative dehydrogenation reaction with iodine to form raw apium celery element. Taking multi-steps recrystallizing, the refined apium celery element would be gained. The invention is easy to operate, stable reacting condition, easy to control. The yield would be 60-70% and the purity could be over 97%. And it is low cost, no harmful to the environment.