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Metabonomics method for detecting multiple flavonoids in fresh tobacco leaves

A technology of metabolomics and fresh tobacco leaves, applied in the field of detection of flavonoids in tobacco, can solve the problems of low accuracy, low cost, and interference of colorimetric results, and achieve good recovery, high sensitivity, and high accuracy Effect

Active Publication Date: 2016-06-22
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

[0004] Some progress has been made in the separation, identification and detection of tobacco flavonoids at home and abroad, but it still lags behind research needs and production practices
At present, there is still a lack of a method system for the comprehensive detection and analysis of the main flavonoids in tobacco. At present, the most commonly used methods for the detection of tobacco flavonoids are colorimetry and chromatography. Colorimetry takes less time and is low in cost. Complicated, there will be other substances that interfere with the results of color contrast, so the accuracy of the results is not high

Method used

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  • Metabonomics method for detecting multiple flavonoids in fresh tobacco leaves
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  • Metabonomics method for detecting multiple flavonoids in fresh tobacco leaves

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Embodiment Construction

[0019] The present invention is further described below in conjunction with specific examples, but does not limit the content of the present invention.

[0020] The embodiment includes standard curve determination, linear range of method, detection limit, limit of quantification, determination of seven flavonoid contents in transgenic tobacco and control samples

[0021] 1. Main experimental materials, instruments and reagents:

[0022] (1) Plant material: The transgenic tobacco leaf material used is from the cultivated tobacco K326 that overexpressed the chalcone synthase gene (CHS) in Yuwang for a long time, which was constructed, verified and preserved by our laboratory in the early stage. The CHS gene is the first key gene in the flavonoid metabolic pathway, overexpression of the CHS gene can increase the content of flavonoids in plants. The leaves of the control tobacco were taken from Yuwangchangchang common K326 tobacco. The leaves obtained from the transgenic tobacco...

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Abstract

The invention relates to detection of flavonoids in tobacco, in particular to a metabonomics method for detecting multiple flavonoids in fresh tobacco leaves.The method is characterized in that flavonoids in the tobacco leaves are extracted through an ultrasonic extraction method, and rutin, quercetin, naringenin, kaempferol glucoside, astragalin, naringin and isoliquiritigenin in the fresh tobacco leaves are detected through UPLC-QqQ-MS / MS.Compared with the prior art, the method has the advantages that accuracy and sensitivity are high, repeatability is good, and the method is suitable for simultaneously and quantitatively detecting the seven flavonoid compounds in plants and can be used for research on metabolic pathways and gene functions of plant flavonoid matter.

Description

technical field [0001] The present invention relates to the detection of flavonoids in tobacco, in particular to a metabonomics method for detecting various flavonoids in fresh tobacco leaves, including rutin, quercetin, naringenin, kaempferol glycosides, astragalin, and naringin Simultaneous quantitative detection of seven flavonoids, isoliquiritigenin, can be used in the research of metabolic pathways and gene functions of plant flavonoids. Background technique [0002] Flavonoids are an important class of plant natural products, which play many functions in plants, such as pigments, transport factors and signaling molecules, and play an important role in plants' resistance to biotic and abiotic stresses. In recent years, flavonoids have received more and more attention. One of the reasons is that plant flavonoid extracts can play an important role in health care in vitro, such as reducing the risk of heart disease and cancer. These properties may be related to the antiox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N30/88G01N2030/027G01N2030/062G01N2030/884
Inventor 金立锋赵明月胡贲周会娜许国旺刘萍萍郑庆霞陈千思陈霞翟妞王燃李锋武明珠林福呈
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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