22 results about "Astragalin" patented technology

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The invention provides a glycosyl transferase gene for catalyzing astragalus smicus glycoside synthesis and application of the glycosyl transferase gene, and belongs to the technical field of biology. The invention develops a novel glycosyl transferase, a novel glycosyl transferase mutant is obtained by utilizing a genetic engineering method, and astragalus smicus glycoside can be synthesized through a microbial fermentation or in-vitro enzyme reaction process. The synthetic route is simpler, the synthetic condition is mild, the environmental pollution is small, the synthetic efficiency is higher, the purity is better, the waste of environmental resources is less, a new synthetic route is provided for the synthesis of the chemical substance, namely, the astragalin with high economic value, and a new thought and method are provided for actual production.

The invention relates to the field of traditional Chinese medicine quality detection, in particular to a method for detecting the content of chemical components in cotton flower medicinal materials. The detection method comprises the following steps: providing a reference substance solution, wherein a reference substance comprises one or more of hyperoside, isoquercitrin, astragalus smicus glycoside, quercetin, quercetin-7-O-glucoside and quercetin-3 '-glucoside; preparing a test solution by using a cotton flower medicinal material sample to be detected; and detecting the reference substance solution and the test solution by high performance liquid chromatography, and determining the content of chemical components corresponding to the reference substance in the to-be-detected cotton flower medicinal material sample, the high performance liquid chromatography meets one or more of the following conditions: a stationary phase is a C18 chromatographic column; the mobile phase comprises a mobile phase A, a mobile phase B and a mobile phase C, the mobile phase A comprises methanol, the mobile phase B comprises acetonitrile, and the mobile phase C comprises a phosphoric acid aqueous solution. The whole method is high in sensitivity, simple and convenient to operate, relatively low in cost and short in detection time.

The present invention relates to the field of traditional Chinese medicine processing technology. The present invention quantifies the three factors of frying temperature, frying time, and auxiliary material dosage, establishes an objective evaluation method, optimizes the processing technology of honey mulberry leaves using the Box-Behnken design-response surface method, detects the index components of honey mulberry leaves using modern instrumental analysis methods, calculates the weight coefficients of seven indicators including chlorogenic acid, rutin, isoquercetin, astragalin, total flavonoids, alcohol extract, and moisture using the CRITIC-AHP composite weighting method, and calculates the overall evaluation "normalization value" to obtain the optimal processing technology results. The final optimized processing process steps are as follows: take 7.5g of refined honey, dilute it with boiling water (mulberry leaf: boiling water = 1:2), pour it into 30g of clean mulberry leaf medicinal materials, mix well, simmer for 4 minutes, place it in a hot pot, fry it at 170°C for 10 minutes, remove it and cool it, and then seal it for storage.

The invention discloses application of an astragalus smicus glycoside preparation for promoting collagen synthesis, and belongs to the field of food and biological medicine cosmetics. The invention provides application of astragalus smicus glycoside or a plant extract containing astragalus smicus glycoside in preparation of drugs, cosmetics or food for preventing and / or delaying skin aging. The skin aging is caused by loss of collagen. Mechanism research shows that the astragalin promotes synthesis of mitochondrial NADPH by activating mitochondrial autophagy genes LGG-1 and DCT-1 and further promotes synthesis of collagen precursor proline, so that synthesis of collagen is promoted, loss of the collagen is reduced, and the effects of resisting skin aging and keeping young and elastic states are achieved. The invention discloses the new application of the astragalus smicus glycoside in promotion of collagen synthesis for the first time, and the application range of the astragalus smicus glycoside is expanded.

The present application belongs to the field of food science and technology, and particularly relates to a method for separating and purifying walnut male flower polyphenols based on macroporous adsorption resin and application. Research shows that the X-5 type resin has the best performance in adsorbing and desorbing walnut male flower polyphenols, the adsorption process conforms to the Temkin isothermal model and the pseudo-second-order kinetic model, indicating that it is a spontaneous exothermic physical adsorption process. After process optimization (80% ethanol, 2 BV / h flow rate, 10 BV elution volume), the polyphenol content is significantly improved. The purified product detects 26 kinds of phenolic acid compounds and 22 kinds of flavonoids, the main components are astragalin, isquercitrin, protocatechuic acid, gallic acid and quercetin, accounting for 89.91% of the total phenol content. The antioxidant activity of the purified polyphenol extract is significantly enhanced, and it has obvious bacteriostatic effect on Staphylococcus aureus. The present application provides a theoretical basis for the high-value utilization of walnut male flower resources and its application in the fields of food and health care.

The present invention discloses a method and application thereof for analyzing the active ingredients of Radix Trichosanthis for treating acute pancreatitis based on HPLC-Q-TOF-MS, network pharmacology, and molecular docking. The analysis method uses HPLC-Q-TOF-MS / MS analysis technology to generate a molecular network of chemical components through accurate mass number, secondary mass spectrometry data, mass spectrometry fragmentation rules, and a database. Then, using a network pharmacology-related database, acute pancreatitis disease-related targets are obtained, a "component-disease-target" network diagram is constructed, a protein interaction network is constructed, and GO function and KEGG pathway enrichment analysis are performed. The 10 key chemical components obtained are: quercetin, coumarin glucoside, gentiopicroside, eriodictyol-7-O-glucoside, protocatechuic acid, 3-O-caffeoylquinic acid, astragalin, isoorientin, isovitexin, and mangiferin.

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a process optimization method for green extraction of hemsley rockvine root flavonoid glycoside through ultrasonic cooperation with a surfactant. The method comprises the following steps: optimizing process parameters for extracting isoorientin, isoquercitrin, kaempferol-3-O-rutinoside and astragalin from radix tetrastigme tuberous roots by using an ultrasonic-assisted lauryl sodium sulfate aqueous solution micelle medium; influences of the type and concentration of a surfactant, a material-liquid ratio, ultrasonic power, ultrasonic time, ultrasonic times and ultrasonic temperature on target components are investigated, and optimal extraction process parameters are determined: the concentration of sodium dodecyl sulfate (SDS) is 35mg / mL, the ultrasonic power is 100W, and the ultrasonic temperature is 50 DEG C. According to the process, a traditional organic solvent is replaced by the surfactant, so that the extraction efficiency is improved; a feasible scheme is provided for green extraction of active ingredients of radix tetrastigme, and industrial amplification feasibility can be further explored subsequently.

The invention provides a feed additive for improving the superovulation effect of cows and a preparation method of the feed additive, and the feed additive comprises the following components in parts by mass: 4-6 parts of astragaloside, 4-6 parts of apigenin, 4-6 parts of kaempferol, 0.4-0.6 part of luteolin, 0.4-0.6 part of isoquercitrin, 0.2-0.4 part of formononetin and 0.2-0.4 part of quercetin. By using the feed additive, the ovulation number and the anti-oxidation and anti-inflammatory capacity of cows can be increased, meanwhile, the natural active ingredients in the feed additive do not need complex chemical synthesis steps, the breeding cost is reduced, animal health is improved, green and safe breeding is guaranteed, and a basis is provided for green development of modern animal husbandry.

The invention discloses a characteristic chromatogram for detecting a Yiyouqing particulate matter basis as well as a construction method and application thereof, and belongs to the field of quality control of traditional Chinese medicine compound preparations. According to the method, hydroxysafflor yellow A, rutin, kaempferol-3-O-rutinoside, astragalus smicus glycoside, flatstem milkvetch glycoside and kaempferol are taken as reference substances, multiple batches of samples of the Yiyouqing granules are analyzed by utilizing a high performance liquid chromatography-mass spectrometry technology, and a chemical component characteristic spectrum is established. Compared with a traditional method, the method has good precision and reproducibility, data are accurate and reliable, the six components can be recognized at the same time under the same chromatographic condition, analysis efficiency is greatly improved, synchronous monitoring of multiple components is achieved, and consistency and stability of preparation quality are guaranteed. The method provides support for improving the quality standard of the Yiyouqing granules, and has important significance on the clinical curative effect and medication safety of the Yiyouqing granules.

The present invention relates to new uses of natural medicines, and particularly to a composition and its application, that is, the application of a pharmaceutical composition in the preparation of a drug for improving or preventing renal fibrosis in diabetic nephropathy, characterized in that the pharmaceutical composition comprises asiatic acid and astragalin. The active components of Cyclocarya paliurus in the present invention, asiatic acid and astragalin, can synergistically balance the TGF-β1 / BMP7 pathway through combined application, block the accumulation of extracellular matrix in renal tubules, and inhibit the process of renal fibrosis in diabetic nephropathy, and the effect is superior to that of the positive drug dapagliflozin group.

The present invention discloses a blackberry flavonol synthase gene RuFLS2 and its application, which belongs to the field of genetic engineering technology. The present application successfully identified and isolated the RuFLS2 gene, whose ORF is 996bp long and encodes a protein composed of 331 amino acids. Spatiotemporal expression analysis showed that RuFLS2 is highly expressed in blackberry fruits, and the expression level reaches a peak when the fruit develops to reddish purple. Heterologous overexpression of tobacco found that the synthesis content of flavonol substances such as naringenin-7-O-glucoside, cyanidin, apigenin-7-glucoside, kaempferol-3-O-rutinoside, astragalin and quercetin increased, indicating that RuFLS2 is a key enzyme gene for regulating the synthesis of blackberry flavonol substances, and can increase the synthesis of flavonols in transgenic plants, and has broad application prospects in the field of plant medicine preparation technology.

The invention provides a thin-layer identification method for flavonoid compounds in humulus lupulus, and belongs to the technical field of traditional Chinese medicine component detection. The thin-layer analysis method for the flavonoid compounds in the humulus lupulus comprises the following steps: adopting a thin-layer chromatography method, taking ethyl acetate, trichloromethane, methanol, water and formic acid in a volume ratio of 7: 2: (0.4-1.2): (0.1-0.9) as a developing solvent, taking a silica gel thin-layer plate as a thin-layer plate, and adopting a color developing agent to identify rutin, isoquercetin and astragalin in the humulus lupulus. According to the present invention, the rapid separation and the qualitative analysis of the rutin, the isoquercetin and the astragalus smicus glycoside in the humulus lupulus are achieved, the separation degree of the rutin, the isoquercetin and the astragalus smicus glycoside is improved, and the trailing phenomenon of the rutin, the isoquercetin and the astragalus smicus glycoside is reduced.

This invention discloses a method for determining the quality of *Hemiberlesia lingua* stems and leaves. The method includes fingerprint spectroscopy establishment and similarity analysis, content determination, cluster analysis, principal component analysis, and partial least squares discriminant analysis. The method of this invention involves UPLC fingerprint analysis and similarity analysis of 15 batches of *Hemiberlesia lingua* stems and leaves from different origins. A total of 28 common peaks were found, and 7 of these common peaks were identified using reference standards: peak 4 (neochlorogenic acid), peak 8 (chlorogenic acid), peak 9 (caffeic acid), peak 11 (cryptochlorogenic acid), peak 22 (isoquercetin), peak 24 (astragalin), and peak 28 (isochlorogenic acid C). This invention's method is highly sensitive, specific, stable, and feasible, providing a reference for the quality evaluation and control of *Hemiberlesia lingua* stems and leaves.

The invention discloses a thin-layer chromatography detection method, quality control and evaluation of pogonatherum crinitum. The thin-layer chromatography detection method comprises the following steps: providing a pogonatherum crinitum test solution; respectively providing reference substance solutions of hyperoside, quercetin, astragalus smicus glycoside and kaempferol; and dripping the test solution and the reference solution on the same thin layer chromatography plate, developing by using a mixed solution of trichloromethane, methanol and formic acid in a volume ratio of (8-12): (1-2.2): (1-2.2) as a developing solvent, taking out, airing, developing, and inspecting. According to the method, rapid separation and identification of hyperoside, quercetin, astragalin and kaempferol in the pogonatherum crinitum are achieved, thin-layer chromatography spots are clear, the accuracy and comprehensiveness of pogonatherum crinitum quality control are improved, and establishment and overall evaluation of pogonatherum crinitum medicinal material quality standards are facilitated.

The invention relates to a composition comprising Astragalin (K) and Miquélianin (Q) in a K / Q mass ratio of between 1 / 1.5 and 1 / 3.5. The invention also relates to the use of a composition according to the invention, in particular to prevent and / or reduce the virulence of S. aureus, and / or to prevent and / or reduce its uncomfortable and / or unsightly manifestations on healthy skin and / or healthy mucous membranes, and / or to treat skin and / or mucous membrane infections caused by S. aureus.

The application belongs to the technical field of health care products, and specifically discloses a composition for enhancing immunity, a health care food and a preparation method, which comprises the following components in parts by weight: 6-10 parts of ganoderma extract, 10-15 parts of astragalin, 12-18 parts of collagen, 18-22 parts of chitosan oligosaccharide, 19-24 parts of polygonatum extract and 24-28 parts of selenium-rich yeast. The ganoderma extract, the astragalin, the collagen, the chitosan oligosaccharide, the polygonatum extract and the selenium-rich yeast are combined, and the synergistic effect of the ganoderma extract, the astragalin, the collagen, the chitosan oligosaccharide, the polygonatum extract and the selenium-rich yeast is utilized to improve the immunity of the human body from different angles, aspects and all aspects.

The application discloses application of a natural product composition in preparation of medicines for preventing or treating azoospermia. The natural product composition is composed of astragalin and curcumin. The natural product composition composed of astragalin and curcumin can inhibit spermatogenic cell apoptosis by synergistically regulating Nrf2 / HO-1 / HIF-1a, and improve rat azoospermia. Compared with western medicines, the natural product composition has a wider treatment window and smaller toxic side effect, and has application prospects in the medicines for preventing or treating azoospermia.

The application discloses a method for detecting the content of multiple components in persimmon calyx samples and application thereof. The detection method comprises the following steps: taking persimmon calyx sample powder, precisely weighing, adding an extraction solvent, performing extraction, centrifugation, taking supernatant, evaporating to dryness, constant volume, filtration, taking the filtrate, and obtaining a test sample solution; the mixed control sample solution and the test sample solution are respectively injected into an ultra-high performance liquid chromatograph, and the characteristic maps of the control sample solution and the test sample solution are compared; the content of the components in the persimmon calyx sample is calculated by using the regression equation of the sample injection amount and the peak area; the components are hyperoside, isoquercitrin, 2"-O-galloyl hyperoside, astragalin, quercetin or kaempferol. The quality detection means of the persimmon calyx sample provided by the application can simultaneously determine the content of six components, is simple in operation, good in reproducibility and stability, high in accuracy, reliable in recovery rate, and high in detection efficiency, and provides a basis for standardizing the persimmon calyx medicinal material and preparation market and reasonably developing.

The invention discloses a rapid analysis and content determination method for components of an alcohol extract of sedum emarginatum, relates to the technical field of quality control of medicinal materials, and solves the problem that the detection efficiency is low when the contents of various components of sedum emarginatum are determined in the prior art. According to the method, under the same chromatographic condition, six components including schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid contained in the sedum emarginatum alcohol extract are subjected to high performance liquid chromatography determination at the same time, and the problem that the detection efficiency is low when the content of various components of sedum emarginatum is determined at present is solved. The content of various components such as sedum emarginatum schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid can be determined under the same high performance liquid chromatography condition, and the determination method is accurate, high in sensitivity, good in repeatability and high in stability.

The application provides a flavoring agent composition and application thereof. The flavoring agent composition of the application comprises menthol, rose essence and astragalin, can not only cover and relieve the taste and smell of Chinese patent medicine preparations when taken, but also can eliminate the gastrointestinal adverse reactions such as vomiting generated after the patient takes the Chinese patent medicine, has remarkable flavoring effect, and has high user satisfaction.

The invention discloses a raspberry salt roasting processing method based on deterministic screening experiment design and a product detection method thereof, the raspberry salt roasting processing method comprises the following steps: adding selected raspberry into a saline solution, uniformly stirring, moistening for 1-3 hours, placing in a stir-frying container, processing at 100-150 DEG C for 1-3 hours, and filtering to obtain the raspberry salt roasting processing method, wherein the mass ratio of raspberry to edible salt is 50: 1, and the use amount of the saline solution is 10-40% of the mass of raspberry; the processing time is 10-30 minutes, cooling, and screening out dust. According to the present invention, the optimal salt roasting processing process condition is obtained by combining the analysis of Design-Expert 12 software, the ellagic acid content and the linden glycoside content after the salt roasting are improved, the content change of the kaempferol-3-O-rutinoside and the astragalus smicus glycoside before and after the salt roasting is not significant, and the scientific basis is provided for the quality standard formulation and the clinical application of the salt-roasted raspberry.