62 results about "Astragalin" patented technology

The present invention is kinder leaf total flavone extract and its preparation process and application, and features that the kinder leaf total flavone extract contains total flavone in 50-95 %, including rutin 0.2-30.0 %, hyperin 0.5-30.0 %, isoquercitrin 10.0-30.0 %, trifolin 3.0-20.0 %, astragaloside 2.0-5.0 %, 6''-O-acetyl isoquercitrin 1.0-6.0 %, quercitrin 0.5-4.0 %, trifolitin 0.2-3.0 %, etc. The kinder leaf total flavone extract is applied in preparing medicine for treating cardiac and cerebral ischemia.

The invention provides a method for enzymatically synthesizing astragalin. The method comprises the following steps that 1, key enzymes needed in the synthesizing process of astragalin are cloned, expressed and purified, wherein the key enzymes comprise glycogen phosphorylase GP, glucose pyrophosphorylase GalU, flavanone-3-hydroxylase F3H, flavanone synthase FLS1 and flavonoid3-O-glucanotransferase UGT78K2; 2, glycogen Gn is synthesized into glucose-1-phosphoric acid G-1-P under the GP effect; 3, G-1-P is synthesized into uridine diphosphate UDPG under the GalU effect; 4, naringenin NRN is synthesized into dihydro kaempferol DHK under the F3H effect; 5, DHK is synthesized into kaempferol KMF under the FLS1 effect; 6, KMF and UDPG are synthesized into astragalin under the UGT78K2 effect. According to the method, few steps are adopted, the operation condition is mild, few side products are generated, the yield is large, pollution is avoided, and the production cost is remarkably reduced.

The invention discloses the application of astragalin in preparation of anti-ovarian-senescence medicaments. Astragalin can treat various ovarian failure diseases, and has a remarkable effect on polycystic ovarian syndrome, dysfunctional uterine bleeding, premature ovarian failure or climacteric syndrome and the like. According to the invention, astragalin is prepared into oral medicaments, percutaneous medicaments or parenteral medicaments for treating ovarian failure diseases.

The invention belongs to the field of traditional Chinese medicine product extraction and separation, particularly relates to an extraction method and application of a red peony synergy whitening extractive, and is used for solving the problems of high content of impurities including pigment and the like and poor whitening effect since the traditional whitening cosmetics in which traditional Chinese medicine extractives are added are generally prepared from traditional Chinese medicine crude extractives. The extraction method of the red peony synergy whitening extractive comprises the following steps: dipping, and carrying out alcohol extraction, concentration, centrifugation and purification to obtain the product. The product contains seven peony monoterpenoid glycoside compounds and four flavonoid compounds, wherein the seven peony monoterpenoid glycoside compounds consist of paeoniflorin sulfurous ester, oxypaeoniflorin, albiflorin std, paeoniflorin, galloyl paeoniflorin, benzoyloxy paeoniflorin and benzoylpaeoniflorin, and the four flavonoid compounds consist of catechinic acid, dihydroquercetin, astragalin and naringenin. The product has a tyrosinase inhibition function, a blood activation function and an oxidation resistance function, and possesses the characteristics of traditional Chinese medicine cosmetic which has a diversity of functional components performing a synergy whitening function.

The present invention relates to a quality control method of ass-hide glue blood-supplementing preparation. Said method is characterized by that in said ass-hide glue blood-supplementing preparation the qualitative identification of pilose asiabell root extract component, cooked rehmannia root extract component and bighead atractylodes rhizome extract component is added, and the quantitative analysis of astragalin content is made, so that said method can make the product quatity obtain effective control.

The invention relates to a separation and preparation method of patuletin-3-O-glucoside and astragalin. The method adopts counter current chromatography to separate and prepare the patuletin-3-O-glucoside and astragalin from alcohol reflux extracts of plant flaveria bidentis; a solvent thereof comprises three components, namely ether or n-alkane or fatty ester, acetonitrile or fatty alcohol or aliphatic ketone and water; the volume ratio is as follows in sequence: 30 to 10:1:30 to 10; and the method is applicable to prepare the monomeric patuletin-3-O-glucoside and astragalin by various types of counter current chromatographs, and can be directly filled with large amount of crude products or synthetic mixtures with the separation purity of more than 95 percent.

The invention relates to a method used for separating flavonoid components of the similar structures. The method is invented based on high performance liquid chromatography; the flavonoid components of the similar structures at least comprise astragalin and quercitrin; the chromatography conditions are as following: octadecylsilane chemically bonded silica is taken as a fixing phase, acetonitrileis taken as a mobile phase A, 0.05 to 0.5% (percent by volume) of a phosphoric acid aqueous solution is taken as a mobile phase B, and gradient elution adopted, 0 to 40min of 7%-25% A, and the balanceB; or 40 to 60min of 25%-50% A, and the balance B. The method is capable of realizing separation of astragalin and quercitrin for the first time. The invention also provides applications of method indetecting flavonoid components in persimmon leaf extract product, and especially applications in measuring of the content of flavonoid components in persimmon leaf extract product or preparations ofpersimmon leaf extract product based on quantitative analysis of multi-components by single-marker.

The present invention discloses the fingerprint method for detecting the quality of astragalus medicine material and its extract. The fingerprint method includes: adopting octodecyl silane bonded silicon gel as filler, acetoitrile-water as flow phase for gradient elution and astragalin A dissolved in methanol as contrast; taking detected astragalus sample through crushing astragalus root, methanol extracting and other steps; detecting the astragalus sample in high efficiency liquid phase chromatographic process to obtain the fingerprint of the detected astragalus sample; and comparing the fingerprint of the detected astragalus sample and the standard fingerprint for similarity degree over 0.90.

The medicine preparation consists of chuanxiongzine or its physiologically acceptable salt and engelitin with astragalin A content not less than 20 wt% in the weight ratio of 0.2-5 as effective medicinal components and pharmacologically acceptable supplementary material. During the preparation process, astragalus root material is processed through water decoction to obtain astragalus root extractive liquid, filtering, adsorption in macroporous resin, washing with water and ethanol solution successively, collecting the ethanol eluted liquid, eliminating ethanol, extraction with n-butanol, alkali solution washing , eliminating n-butanol, and reflux with acetone to defat to obtain engelitin. Engelitin is then mixing with chuanxiongzine compound and corresponding supplementary material to prepare the medicine preparation. Test shows that the medicine preparation has obvious synergistic effect.

The invention relates to the field of extraction of effective components of traditional Chinese medicines and discloses a method for extracting total flavonoid in pteridium aquilinum by an ultrasound-assisted compound enzyme method. The method comprises the following steps: (1) carrying out fixation on fresh pteridium aquilinum, drying, crushing and sieving to obtain dry powder; (2) putting the pteridium aquilinum dried powder into a conical flask; adding ethanol according to the ratio and extracting for 15 to 25min under the condition that the ultrasonic power is 280 to 320W; extracting for 2to 4 times, wherein the time interval is 14 to 16min; (3) cooling to room temperature and adding a compound enzyme; then extracting for 110 to 120min under the condition that the temperature is 50 to55 DEG C; filtering and collecting filtrate; (4) decompressing and concentrating until paste is formed, and freezing and drying; (5) carrying out polyamide column purification, freezing and drying. The method disclosed by the invention has the advantages that the ultrasound-assisted compound enzyme method is adopted; in the extracted pteridium aquilinum, the content of flavonoid is 9.0 to 9.2 percent, the content of rutin is 0.95 to 1.2 percent and the content of astragalin is 0.80 to 0.90 percent.

The invention discloses application of astragalin in preparation of a drug for treating diabetic testicular dysfunction. An experimental result shows that when the astragalin is used in a safety doserange, the dose is 30 mg/kg of astragalin, the astragalin can remarkably protect sperm injury induced by a diabetes model, improve pathologic change of testicular tissue, improve the oxidation resistance ability and regulate and control protein expression of inflammatory cytokines, and it is proved that the astragalin has the effect of improving the diabetic testicular dysfunction.

The invention discloses a high-temperature resistant flavonoid aglycone invertase and applications thereof. Amino acid sequence of the high-temperature resistant flavonoid aglycone invertase is represented by SEQ ID No.1. Thermal stability of the high-temperature resistant flavonoid aglycone invertase is excellent; the high-temperature resistant flavonoid aglycone invertase is capable of enduring high temperature, and possesses relatively excellent catalytic conversion capability on flavone glycosides of different mother nucleus types and different glucosidic bond types. After a certain time of incubation of the high-temperature resistant flavonoid aglycone invertase with typical flavone glycosides (isoquercetin, astragalin, daidzin, and saprosma ternatum glucoside), it is observed via detection that almost all of the flavone glycosides are converted into corresponding flavonoid aglycones (quercetin, kaempferol, isoflavoues aglycone, and genistein).

The invention provides a preparation method of a hamamelis virginiana extract. The method employs a two-step method to extract quercetin and astragalin in hamamelis virginiana respectively. In the first step, water-acid coarse extraction is carried out, then 65-70% ethanol is utilized for fine extraction at 70-75DEG C so as to obtain quercetin finally, and the quercetin extraction rate reaches 0.75%; the second step adopts ultrasonic alcohol extraction to extract astragalin, under an ultrasonic condition, astragalin in hamamelis virginiana can be dissolved in a high concentration alcohol solution. The method provided by the invention has clear extraction target, can effectively extract quercetin and astragalin in hamamelis virginiana, has high extraction rate, and the obtained extract canbe applied to acne removing cosmetics, can be absorbed by skin, and synergistically reach the efficacy of efficient bacteriostasis, inflammation resistance and acne removal.

The invention relates to an air conditioning plastic with functions of high temperature resistance and oxidation resistance. The formula of the air conditioning plastic comprises raw materials in parts by weight as follows: 100 parts of PE (polyethylene), 15 parts of silkworm cocoon hydrolyzed extracts, 8 parts of hydroxyl silicone oil, 10 parts of turpentine, 5 parts of butyl phosphate, 2.5 parts of an antioxidant, 2.5 parts of a cross-linking agent, 2 parts of silicon dioxide, 1.5 parts of astragalin, 1.5 parts of an aminopolysiloxane emulsion and 1.5 parts of conchiolin. The plastic has functions of high temperature resistance and oxidation resistance and is particularly applicable to an air conditioning casing.

The invention discloses a group of DNA molecules and recombinant plasmids thereof as well as recombinant escherichia coli and an application of the recombinant escherichia coli in producing astragalin. The DNA molecules include an arabidopsis glycosyltransferase gene 78D2, a sucrose phospholylase gene Basp, a uridyltransferase ugpA and a sucrose permease gene cscB which are optimized; and the nucleotide sequences of the genes are separately shown as SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:6. The recombinant bacterium (the recombinant escherichia coli) can achieve the efficient expression of complete-gene optimized glycosyltransferase gene op78D2, and glycosyltransferase can efficiently and specifically conduct glycosylation on kaempferol 3rd-site hydroxyl; and meanwhile, by enhancing a UDP-glucose synthesis route of the recombinant bacterium, the recombinant bacterium can efficiently supply UDP-glucose, so that the bottleneck of the recombinant bacterium in synthesizing the astragalin is relieved. Finally, by optimizing fermentation conditions, the efficient synthesis of the astragalin is achieved.

The invention relates to biogenic controlled-release sterilization liquid and a preparation method and application thereof, belonging to the technical field of biopesticides. The biogenic controlled-release sterilization liquid is characterized by taking methyl alcohol as a solvent, wherein per kilogram of methyl alcohol contains the following active ingredients: 160-280g of sinomenine, 50-170g of kaempferol, 70-180g of astragalin, and 100-240g of gentiopicroside, and also contains 70-230g of penetrant, and 100-300g of controlled-release additive. The biogenic controlled-release sterilization liquid takes natural substances in a plant as the active ingredients, can control the release rate of pesticide by the pesticide release rate of the controlled-release agent, can resist bacteria continuously and efficiently, can reduce photolysis and losses of active substances in environment, avoids peak valley fluctuation of pesticide concentration, enables the pesticide to play a role smoothly and continuously so as to reduce the dosage of the pesticide and prolong the effective duration. The biogenic controlled-release sterilization liquid has the advantages of being convenient to manufacture, low in cost, stable and efficient.

The invention discloses an HPLC fingerprint for the ethyl acetate position of a longan leaf and an establishing method of the HPLC fingerprint. The method comprises the steps that a solution of the tested object is prepared; a contrast solution including ethyl gallate, astragalin and quercetin is prepared; and HPLC is used for determination to obtain the fingerprint. The method has the advantagesof being rapid, accurate, high in precision, repeatability and stability, abundant in chromatographic peak information, and good in separating effect. The established HPLC fingerprint for the ethyl acetate position of the longan leaf can effectively represent the quality of the longan leaf, especially the quality of the ethyl acetate position of the longan leaf, and furhter the medical standard ofthe longan leaf is improved, the longan leaf quality evaluation system is improved, standardization of traditional Chinese medicines is facilitated, theoretical and practical basis is provided for comprehensive and effective control for the longan leaf quality, and accurate reliable basis is provided for the safety and effectiveness of clinical medicine.

A medical composition for preventing and treating infection and viral diseases, antipyressis, and analgesia is prepared from sodium neohouttuyfonate or its pharmacologically acceptable salt (1-20 portions) and scutelloside (30-500 portions). Its preparing process is also disclosed.

The invention relates to a natural medicine composition, a preparation method thereof, and an application thereof in preparing an attenuating and synergic medicine used in cancer chemotherapy. The natural medicine composition is composed of a Mongolian milkvetch root and Baikal skullcap root water decoction concentrate extract, astragalin, Baikal skullcap root total flavonoid, and baicalin according to a ratio of 2-5:2-6:3-7:4-7. The composition provided by the invention has an excellent treatment effect against cancer-chemotherapy-related diarrhea, and provides a synergetic effect for cancer chemotherapy.

The invention discloses a pharmaceutical composition for treating chronic gastroenteritis, which is prepared from the following raw materials: belladonna and aluminium dispersible tablets, levocarnitine, bismuth potassium citrate, metronidazole, ranitidine hydrochloride, vitamin B, lovastatin, niacin, calcium pantothenate, niacinamide, L-arginine, jujube polysaccharide, cassia seed, fructus crataegi, radix et rhizoma salviae miltiorrhizae, radix et rhizoma rhei, ginger, tartary buckwheat, radix puerariae lobatae, leucocyanidin, vitexin, astragalin, cryptoxanthin and quercetin. The pharmaceutical composition for treating chronic gastroenteritis has the synergistic effects through the compounding of the raw materials, has obvious anti-inflammatory activity, and has the advantages of both symptoms and roots treatment of the disease, obvious curative effects, convenient taking, no recurrence and no rebound after cure, little toxic and side effects, no complication, long-term use, less residues and low price, so that the production cost is reduced and the waste of resources is avoided.

The invention relates to the field of medicine, in particular to application of a tilia amurensis inflorescence extractive to preparation of a medicine for treating insomnia. A preparation method of the tilia amurensis inflorescence extractive comprises the following steps: extracting tilia amurensis inflorescence by using 70% ethyl alcohol, then extracting the tilia amurensis inflorescence by using ethyl acetate to prepare a crude extractive, carrying out gradient elution on the crude extractive through a dichloromethane-methyl alcohol mixed solution by using a silica gel column chromatography, collecting eluant prepared by the dichloromethane-methyl alcohol mixed solution with dichloromethane and methyl alcohol in a volume ratio of 15 to 1, and recycling a solvent to prepare a purified extractive, wherein the purified extractive of the tilia amurensis inflorescence is separated by an ODS column chromatography and then is eluted by a 50% methanol aqueous solution to obtain two monomeric compounds; by virtue of NMR identification, the two monomeric compounds are tiliroside and astragalin; the tiliroside is an active component; the purified extracted is analyzed by HPLC; the mass content of the tiliroside is 31.7%. Animal experiments show that the tilia amurensis inflorescence extractive is obvious in resistance to the insomnia; the dose-effect relationship research shows that the tilia amurensis inflorescence extractive is free of obvious dose dependence and low in addiction possibility; the safety of the tilia amurensis inflorescence extractive is superior to that of commonly-available chemical medicines at present.

The invention belongs to the field of medicine and particularly relates to in-situ gel for subcutaneous injection for treating breast cancer and its pharmaceutical use. The active ingredient of the in-situ gel for subcutaneous injection is astragalin or a composition of astragalin and laurolitsine in the weight ratio of the astragalin to laurolitsine is 63 to 69: 19. The excipients of the in-situgel for subcutaneous injection comprise soy lecithin, medium chain triglyceride and 90% ethanol. The in-situ gel for subcutaneous injection for treating breast cancer exerts a sustained release effectby a gel drug reservoir formed by subcutaneous injection, has a good therapeutic effect on estrogen-dependent breast cancer and can reduce toxic or side effects caused by systemic administration suchas oral administration or intravenous injection.

The invention discloses a method for separating astragalus polysaccharide and astragalin astragaloside. The method is characterized by adopting the process combining the separation of an ultrasonic film separator and the separation of reversed-phase preparative column chromatography. The whole technological process is simple, quick and large in processing amount and purifies two active ingredients, namely the astragalus polysaccharide and the astragalin astragaloside, at the same time. The obtained product has high purity. The method has the advantages of simple process, high automation control degree and simple industrial application.