81 results about "Neochlorogenic acid" patented technology

The invention discloses a quality detection method for carpesium abrotanoides formula granules, and belongs to the technical field of traditional Chinese medicine detection. The detection method comprises the following steps: testing the total content of the specific chromatogram, neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid, and testing the content of carabrone. The method for testing the specific chromatogram and the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid comprises the following steps: a, preparing a neochlorogenic acid reference substance solution, a chlorogenic acid reference substance solution and a cryptochlorogenic acid reference substance solution; b, preparing a test solution; and c, carrying out liquid chromatography detection, and calculating the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid. The carabrone content test comprises the following steps: A, preparing a carabrone reference substance solution; b, preparing a test solution; and C, carrying out liquid chromatography detection, and calculating the carabrone content. The quality of the carpesium abrotanoides formula granules is effectively controlled, and the medication effectiveness and safety of the carpesium abrotanoides formula granules are guaranteed.

The present invention provides an oriental cherry flower extract containing 200 mg / kg or more of neochlorogenic acid as an active ingredient. The oriental cherry flower extract provided by the invention can promote the degradation of saccharification products, and can improve cell oxidative stress or inflammatory response caused by the saccharification products.

The invention belongs to the field of quality control of modern traditional Chinese medicines, and discloses a quality control method for a mixing process of intestine-strengthening and diarrhea-stopping pills based on combination of NIRS spectrum and MSPC technology. The method comprises the following steps: pre-treating medicinal materials according to a prescription proportion; an NIRS spectrometer is adopted to collect data; performing variable expansion and dimensionality reduction on the three-dimensional spectrum data, establishing an MSPC model in batches based on a training set, and setting a control limit according to a standard deviation + / -3 times of a mean value of a principal component score (PC1), Hotelling Tand DModX statistical magnitude; and a new batch of spectrum data is input in real time, and feeding abnormity and process abnormity are jointly monitored through three types of statistics. A PLS quantitative model is synchronously established, and dynamic content prediction of the six chemical components such as neochlorogenic acid is achieved. According to the method, real-time early warning of abnormity in the mixing process and dynamic analysis of chemical components are achieved, and the uniformity and stability of the quality of the intestine-strengthening and diarrhea-stopping pills are remarkably improved.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method of specific chromatograms of common carpesium fruit and a preparation thereof. Comprising the following steps: by taking octadecylsilane chemically bonded silica as a filling agent and a mobile phase comprising methanol and an acid-containing aqueous solution, carrying out gradient elution, and screening through a large number of experiments to obtain a specific elution procedure, so as to obtain 13 or more common characteristic peaks, and realizing the purification of the chlorogenic acid, the caffeic acid, the chlorogenic acid, the cryptochlorogenic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid and the 1, 4-dicaffeoylquinic acid. The method can effectively separate characteristic peaks of 2, 5-dicaffeoylquinic acid, cosmosiin and carabrone, is good in peak shape, stable in base line, short in detection time, provides a basis for quality detection and control of the common carpesium fruit and the preparation thereof, can fully reflect the integrity and characteristics of the common carpesium fruit and the preparation thereof, is simple to operate, and is suitable for industrial production. The method is suitable for quality control of different preparation types of common carpesium fruit.

The invention provides a folium ilicis cornutae extract as well as a preparation method and application thereof. The core component of the holly leaf extract comprises polyphenol compounds, the total polyphenol content is 10-500mg / g, and the polyphenol components comprise neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoquercitrin, isochlorogenic acid B, isochlorogenic acid C and isochlorogenic acid A according to a ratio of (95-100): (300-450): (70-109): (120-256): (20-46): (21-156): (24-43): (27-61). The preparation method of the Chinese holly leaf extract comprises the whole processes of raw material pretreatment, multi-solvent synergistic extraction, multi-stage double-effect filtration and impurity removal, deep concentration and drying, parameters of all links are accurately controlled, the method is high in extraction rate, and the obtained Chinese holly leaf extract can effectively reduce the uric acid level and prevent and treat hyperuricemia.

The invention discloses a construction method of a characteristic chromatogram of a rhizoma dryopteris crassirhizomae medicinal material, formula granules, decoction pieces or standard decoction, and the construction method comprises the following steps: preparing a reference substance solution which comprises a reference substance solution of a reference medicinal material and a reference substance solution of a reference substance, the reference substance solution comprises a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance and a chlorogenic acid reference substance; taking a rhizoma dryopteris crassirhizomae medicinal material, formula granules, decoction pieces or a standard decoction to prepare a test solution; a preset amount of the reference substance solution and the test solution are taken and injected into a liquid chromatograph, the liquid chromatograph takes octadecylsilane chemically bonded silica as a filler, methanol as a mobile phase A and a phosphoric acid solution as a mobile phase B for gradient elution, and the characteristic chromatogram is established. The method can provide a data basis for quality control of rhizoma dryopteris crassirhizomae medicinal materials, formula granules, decoction pieces or standard decoction, and ensures the stability and controllability of product quality.

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The invention discloses construction and application of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum for integrally controlling a multi-component material basis of a traumatic injury bone-growing capsule, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and neochlorogenic acid, protocatechuic aldehyde, cryptochlorogenic acid, vanillic acid, rosmarinic acid, alkannic acid, berberine hydrochloride and salvianolic acid B are taken as reference substances; the fingerprint spectrum is successfully constructed by detecting different batches of traumatic injury bone-growing capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least eight components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be carried out on a plurality of characteristic components of the traumatic injury bone-growing capsule accurately, rapidly and simultaneously, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the traumatic injury bone-growing capsule.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The application relates to the field of traditional Chinese medicine quality control, and particularly discloses a quality control method for Shuanghuanglian inhalation solution. The method can simultaneously determine the fingerprint spectrum and ingredient content of the Shuanghuanglian inhalation solution. The fingerprint spectrum detection method disclosed by the application adopts UPLC determination, has 17 characteristic peaks, and attributes 8 characteristic peaks. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and forsythoside are simultaneously determined. Compared with the prior art, the method for determining the fingerprint spectrum and content of the Shuanghuanglian inhalation solution is improved, and the quality of the Shuanghuanglian inhalation solution can be comprehensively monitored conveniently, quickly and accurately.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to an establishment method of a mulberry paste fingerprint spectrum and the fingerprint spectrum. The establishment method of the mulberry paste fingerprint spectrum comprises the following steps: preparing a test solution by taking mulberry paste as a raw material, and respectively preparing reference solutions by taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethylfurfural, rutin and isoquercitrin; respectively injecting the test solution and the reference solution into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting the chromatograms of the test solutions of multiple batches of mulberry paste, introducing the chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, generating a reference map, and calculating the similarity by taking the peak area and retention time as parameters. The method can be used for simultaneously detecting a plurality of components in the mulberry paste, and can be effectively used for evaluating the product quality stability of the mulberry paste.

The invention discloses a method for separating high-purity chlorogenic acid and neochlorogenic acid from a stevia rebaudiana chlorogenic acid product based on membrane coupling extraction crystallization. The method comprises the following steps: preparing a first to-be-treated solution by taking the stevia rebaudiana chlorogenic acid product as a raw material; treating the first to-be-treated liquid through an ultrafiltration membrane and a nanofiltration membrane in sequence to obtain a trapped fluid II, concentrating the trapped fluid II, and adjusting the pH value to obtain a second to-be-treated liquid; extracting the second to-be-treated liquid with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase I and an extract phase I; adjusting the pH of the raffinate phase I to obtain a third to-be-treated solution; extracting the third to-be-treated liquid with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase II and an extract phase II; drying the extract phase I, crystallizing, pulping and purifying to obtain high-purity chlorogenic acid; and drying the extract phase II, crystallizing, pulping and purifying to obtain the high-purity neochlorogenic acid. According to the method, membrane separation, solvent extraction and fractional crystallization are coupled, and high-efficiency and high-selectivity separation and purification of chlorogenic acid and neochlorogenic acid in a stevia rebaudiana chlorogenic acid product are realized.

The present invention provides a method for constructing a characteristic spectrum of a Glechoma galbana medicinal material, a standard decoction, or a traditional Chinese medicine formula granule, comprising the following steps: preparing a chlorogenic acid reference substance and a chlorogenic acid reference substance solution; preparing a mixed reference substance solution and a neochlorogenic acid and cryptochlorogenic acid reference substance solution; preparing a test solution and subjecting the chlorogenic acid reference substance solution, the mixed reference substance solution, and the test solution to ultra-high performance liquid chromatography analysis, respectively; wherein the ultra-high performance liquid chromatography analysis conditions include: mobile phase A is acetonitrile, mobile phase B is aqueous phosphoric acid, and a gradient elution procedure is employed. The characteristic spectrum constructed by the method described in the present invention is rich in information, highly characteristic, and has good reproducibility.

The invention provides a method for establishing a fingerprint spectrum of a bock greenbrier rhizome preparation, which comprises the following steps: S1, preparation of a test solution: weighing the bock greenbrier rhizome preparation, adding methanol, carrying out ultrasonic treatment, and filtering to obtain the test solution; s2, preparation of a reference substance solution: weighing eight reference substances including cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, polydatin, astilbin, oxyresveratrol, quercitrin and resveratrol, mixing, and adding methanol to prepare the reference substance solution; and S3, establishment of a fingerprint: respectively measuring the prepared test solution and the reference solution by using HPLC, recording and comparing chromatograms, and identifying common peaks to obtain the fingerprint of the bock greenbrier rhizome preparation. According to the method, the fingerprint spectrum of the bock greenbrier rhizome preparation is successfully established by optimizing the extraction conditions of the bock greenbrier rhizome preparation and the HPLC chromatographic conditions, the establishment method of the fingerprint spectrum is accurate, reliable and simple to operate, and the detection method is high in precision and good in specificity, stability and repeatability.

The invention discloses a method for continuously preparing neochlorogenic acid through a two-phase system, and belongs to the technical field of plant byproduct utilization. The method comprises the following steps: dissolving a raw material containing isochlorogenic acid in ethyl acetate, adding a proper amount of water, adding alkali to adjust the pH value to 7-11, and carrying out a first hydrolysis reaction in a water-ethyl acetate two-phase system reaction solution; after the first hydrolysis reaction is finished, adjusting the pH value of a reaction liquid water layer to 2-6, and separating the water layer from an ethyl acetate layer; adding water into the ethyl acetate layer, and continuing to repeat the hydrolysis reaction; collecting and combining water layers separated each time, performing macroporous adsorption resin purification and methanol desorption on the water layers, collecting desorption liquid, concentrating and spraying to obtain the neochlorogenic acid. The total content of chlorogenic acid in the prepared product can reach 75% and above, and the content of neochlorogenic acid 5-CQA can reach 50% and above. The method has the advantages of simple process, easiness in operation and the like, and is suitable for large-scale industrial production.

The invention discloses application of neochlorogenic acid in preparation of a medicine for preventing and / or treating hand-foot-and-mouth disease caused by A16 type coxsackie virus (CVA16) infection, and belongs to the technical field of biological medicine. Researches prove that the neochlorogenic acid has the effect of resisting CVA16 infection on HEK293T cells and H4 cells, after the two cells treated by the neochlorogenic acid are infected by the CVA16, the viral genome RNA abundance is obviously reduced, and the cell lesion death condition is obviously relieved; meanwhile, a cytotoxicity test shows that the neochlorogenic acid has no obvious cytotoxicity to HEK293T cells and only has weak cytotoxicity to H4 cells. Therefore, in a reasonable dosage range, the neochlorogenic acid, which is a low-toxicity natural small molecule compound, can be used for preparing the anti-CVA16 medicine for preventing and / or treating the hand-foot-and-mouth disease caused by CVA16 infection.

The present application relates to a kind of Huahong composition effective part extract and its preparation method, the Huahong composition effective part extract includes the following components: syringic acid, new chlorogenic acid, deacetyl carlina acid methyl ester, geniposidic acid, N-acetyl-L-tyrosine, fraxidin, 4-pyridoxinic acid, 7,8-dihydroxy-4-methyl coumarin, chlorogenic acid, ferulic acid;The total content of above components is 80%-95% of effective part extract.The Huahong composition effective part extract of the present application is from the effective part of Huahong composition.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The invention relates to the technical field of traditional Chinese medicine, in particular to a composition, application of the composition in preparation of medicine for treating liver cancer or / and breast cancer and a screening method. According to the invention, the composition is obtained through screening by combining the hemogenic component of the marsdenia tenacissima tablets with targeted network pharmacological research, and the composition comprises neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, marsdenia tenacissima glycoside I and marsdenia tenacissima glycoside H. Each active component in the composition corresponds to a plurality of targets, each target is connected with a plurality of active components, and the plurality of targets correspond to a plurality of pathways, so that the plurality of active components in the composition act on the liver cancer or / and the breast cancer from the plurality of targets and the plurality of pathways in a synergistic manner, and a new research direction is provided for developing medicines for treating the liver cancer or / and the breast cancer.

Provided herein are polyphenol-enriched lettuce powders comprising particles of a polyphenol-enriched lettuce or extract, wherein the polyphenol-enriched lettuce comprises from 40-280 mg / g of polyphenols. The polyphenols include any combination of a chlorogenic acid, a neochlorogenic acid, a chicoric acid, a quercetin, a quercetin derivative, and an anthocyanin. The disclosure also provides compositions and methods of using the polyphenol-enriched lettuce powders to promote wound healing and treat inflammation and skin disorders.

The invention belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a quantitative analysis method for effective components of common goldenrop medicinal materials. The method comprises the following steps: a, preparation of a reference substance solution: precisely weighing neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, 3, 5-O-dicaffeoylquinic acid, quercitrin and 4, 5-O-dicaffeoylquinic acid reference substances, and adding methanol to prepare a mixed reference substance solution; b, preparation of a test solution: crushing a common goldenrop herb, adding a methanol solution for extraction, and filtering to obtain the test solution; and c, determination: respectively and precisely sucking the reference substance solution and the test solution, injecting into an ultra-high performance liquid chromatograph, determining, and calculating the content by adopting an external standard method. The method can simultaneously detect and analyze the contents of seven chemical components in the common goldenrop herb, has good linearity, repeatability, stability and recovery rate, and can effectively control the quality of the common goldenrop herb.

The invention belongs to the technical field of medicinal material quality control, and discloses a honeysuckle flower QAMS (quantitative analysis of multi-components by single marker) quality control method, which comprises the following steps: preparing a single reference solution and a mixed reference solution of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C, and preparing a test solution; after high performance liquid chromatography detection, the relative correction factors of other components in the honeysuckle are calculated by taking chlorogenic acid as an internal reference substance through chromatographic peak positioning, and the contents of the components in the honeysuckle are obtained by utilizing the relative correction components, so that the synchronous determination of the multiple components in the honeysuckle is realized. The method has the advantages of cost saving, simplicity in operation, high detection accuracy and good stability, has great significance in quality control and clinical curative effect guarantee of the honeysuckle medicinal material, and provides a basis for establishing a quality evaluation standard of the honeysuckle medicinal material.

The present application relates to the technical field of traditional Chinese medicine detection, and provides a genuine three-leafed knot thin-layer identification kit and method, comprising: a chrysophanol control solution, a neochlorogenic acid control solution and a rapid extraction solvent, the rapid extraction solvent contains a low-carbon alcohol, which is used for extracting flavonoid glycoside components in three-leafed knot stem and leaf samples, and the kit is used for identifying the origin of three-leafed knot by detecting the content of chrysophanol and the presence of neochlorogenic acid. The present application uses a chrysophanol and neochlorogenic acid double-index mutual exclusion identification logic, combined with a rapid extraction solvent system without drying, to realize on-site, rapid and accurate identification of the genuineness of three-leafed knot, and effectively solves the problem of origin confusion.

The application discloses application of a medicine composition in preparation of a medicine for treating rheumatoid arthritis (RA), and belongs to the field of RA treating medicines. The medicine composition is composed of swertiamarin (SW) and neochlorogenic acid (NCA) and is screened from two active ingredients in Danteng Yimu Decoction. With the help of animal model and cell model experiments, the action mechanism of the medicine composition in treating RA is explored, the related symptoms and indexes of collagen-induced arthritis (CIA) rats can be effectively improved, joint swelling can be relieved, arthritis inflammation index can be reduced, inflammatory factor release can be inhibited, joint bone quality can be protected, arthritis inflammation infiltration and cartilage destruction can be reduced, and the medicine safety is good. The composition can effectively inhibit the inflammatory factor release and migration ability of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). Animal experiments and cell experiments prove that the combination of neochlorogenic acid and swertiamarin (SW+NCA) can be effectively applied to clinical treatment of RA and has a wide application prospect.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The present invention proposes a method for simultaneously determining the contents of six active ingredients of the Zhuang medicine Sophora flavescens by HPLC, which belongs to the technical field of traditional Chinese medicine analysis. The method comprises the following steps: S1. Preparation of standard stock solution; S2. Preparation of standard mixed solution; S3. Preparation of test solution; S4. Chromatographic conditions; S5. Determination. The present invention compares and analyzes the content determination results of 10 batches of samples and finds that there are differences in the average contents of the six components of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C in different batches of Sophora flavescens medicinal materials. The present invention establishes a method for simultaneously determining the contents of the six components of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C in the Zhuang medicine Sophora flavescens, which will provide a theoretical basis for the quality control and standardized planting of Sophora flavescens.

The present invention discloses a method for constructing a characteristic spectrum of a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction, comprising the following steps: preparing a reference solution, the reference solution comprising a control medicinal material reference solution and a reference substance reference solution, the reference substance reference solution comprising a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance, and a chlorogenic acid reference substance; taking a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction to prepare a test solution; taking a preset amount of the reference solution and the test solution, injecting them into a liquid chromatograph, the liquid chromatograph using octadecylsilane bonded silica gel as a filler, methanol as mobile phase A, and phosphoric acid solution as mobile phase B for gradient elution to establish a characteristic spectrum. The present invention can provide a data basis for the quality control of Corydalis guanzhong medicinal materials, formula granules, decoction pieces or standard decoctions, and ensure the stability and controllability of product quality.

The invention relates to the technical field of medicine quality control, and particularly discloses a quality control method of honeysuckle and radix scutellariae buccal dripping pills based on a fingerprint spectrum, which comprises three steps of preparation of a test solution, preparation of a reference solution and chromatographic detection. On the basis that only chlorogenic acid and baicalin are detected originally, cryptochlorogenic acid peaks, neochlorogenic acid peaks and baicalein peaks are also included in the quality control standard of the honeysuckle and radix scutellariae buccal dripping pills, so that the problem that in the prior art, the quality control of different batches of honeysuckle and radix scutellariae buccal dripping pills takes the content of chlorogenic acid and baicalin as the current quality standard; and quality control cannot be carried out on products with the same content of chlorogenic acid and baicalin and different curative effects.

The present invention provides a method for simultaneously determining the contents of 8 components in Xiongju Shangqing Tablets. The HPLC method is used in the present invention to determine neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid. The HPLC method is adopted in the present invention to establish a method for simultaneously determining the contents of 8 components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid in Xiongju Shangqing Tablets. The method is simple and highly specific, providing a basis for the establishment of the quality standard of Xiongju Shangqing Tablets. The established method can play a role in process control to a certain extent and provide a reference for the quality control and quality evaluation of the product.

The invention belongs to the technical field of traditional Chinese medicine component detection and analysis, and particularly relates to a method for detecting various active components in lonicerae flos by quantitative analysis of multi-components by single marker. According to the QAMS detection method for the various active ingredients in the lonicera confusa, based on a simple, convenient and rapid high performance liquid chromatography technology, QAMS of the active ingredients such as flavonoids, flavonoid glycosides, iridoid glycosides and organic acids in the lonicera confusa can be achieved, and qualitative and quantitative detection of the various active ingredients is included. The detection target objects comprise 14 active components, namely neochlorogenic acid, cumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, swertiamarin, secologanin, rutin, quercitrin, galuteolin, ferulic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C. The invention further provides a method for detecting the content of the isochlorogenic acid. Tests prove that the method disclosed by the invention meets methodological requirements, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, accuracy in determination and strong applicability, is beneficial to accurately evaluating the quality of the lonicerae flos and provides a reliable basis for safe application of the lonicerae flos.