47 results about "Neochlorogenic acid" patented technology

The present invention provides an oriental cherry flower extract containing 200 mg / kg or more of neochlorogenic acid as an active ingredient. The oriental cherry flower extract provided by the invention can promote the degradation of saccharification products, and can improve cell oxidative stress or inflammatory response caused by the saccharification products.

The invention belongs to the field of quality control of modern traditional Chinese medicines, and discloses a quality control method for a mixing process of intestine-strengthening and diarrhea-stopping pills based on combination of NIRS spectrum and MSPC technology. The method comprises the following steps: pre-treating medicinal materials according to a prescription proportion; an NIRS spectrometer is adopted to collect data; performing variable expansion and dimensionality reduction on the three-dimensional spectrum data, establishing an MSPC model in batches based on a training set, and setting a control limit according to a standard deviation + / -3 times of a mean value of a principal component score (PC1), Hotelling Tand DModX statistical magnitude; and a new batch of spectrum data is input in real time, and feeding abnormity and process abnormity are jointly monitored through three types of statistics. A PLS quantitative model is synchronously established, and dynamic content prediction of the six chemical components such as neochlorogenic acid is achieved. According to the method, real-time early warning of abnormity in the mixing process and dynamic analysis of chemical components are achieved, and the uniformity and stability of the quality of the intestine-strengthening and diarrhea-stopping pills are remarkably improved.

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The invention discloses construction and application of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum for integrally controlling a multi-component material basis of a traumatic injury bone-growing capsule, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and neochlorogenic acid, protocatechuic aldehyde, cryptochlorogenic acid, vanillic acid, rosmarinic acid, alkannic acid, berberine hydrochloride and salvianolic acid B are taken as reference substances; the fingerprint spectrum is successfully constructed by detecting different batches of traumatic injury bone-growing capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least eight components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be carried out on a plurality of characteristic components of the traumatic injury bone-growing capsule accurately, rapidly and simultaneously, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the traumatic injury bone-growing capsule.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The application relates to the field of traditional Chinese medicine quality control, and particularly discloses a quality control method for Shuanghuanglian inhalation solution. The method can simultaneously determine the fingerprint spectrum and ingredient content of the Shuanghuanglian inhalation solution. The fingerprint spectrum detection method disclosed by the application adopts UPLC determination, has 17 characteristic peaks, and attributes 8 characteristic peaks. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and forsythoside are simultaneously determined. Compared with the prior art, the method for determining the fingerprint spectrum and content of the Shuanghuanglian inhalation solution is improved, and the quality of the Shuanghuanglian inhalation solution can be comprehensively monitored conveniently, quickly and accurately.

The invention discloses a method for separating high-purity chlorogenic acid and neochlorogenic acid from a stevia rebaudiana chlorogenic acid product based on membrane coupling extraction crystallization. The method comprises the following steps: preparing a first to-be-treated solution by taking the stevia rebaudiana chlorogenic acid product as a raw material; treating the first to-be-treated liquid through an ultrafiltration membrane and a nanofiltration membrane in sequence to obtain a trapped fluid II, concentrating the trapped fluid II, and adjusting the pH value to obtain a second to-be-treated liquid; extracting the second to-be-treated liquid with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase I and an extract phase I; adjusting the pH of the raffinate phase I to obtain a third to-be-treated solution; extracting the third to-be-treated liquid with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase II and an extract phase II; drying the extract phase I, crystallizing, pulping and purifying to obtain high-purity chlorogenic acid; and drying the extract phase II, crystallizing, pulping and purifying to obtain the high-purity neochlorogenic acid. According to the method, membrane separation, solvent extraction and fractional crystallization are coupled, and high-efficiency and high-selectivity separation and purification of chlorogenic acid and neochlorogenic acid in a stevia rebaudiana chlorogenic acid product are realized.

The present invention provides a method for constructing a characteristic spectrum of a Glechoma galbana medicinal material, a standard decoction, or a traditional Chinese medicine formula granule, comprising the following steps: preparing a chlorogenic acid reference substance and a chlorogenic acid reference substance solution; preparing a mixed reference substance solution and a neochlorogenic acid and cryptochlorogenic acid reference substance solution; preparing a test solution and subjecting the chlorogenic acid reference substance solution, the mixed reference substance solution, and the test solution to ultra-high performance liquid chromatography analysis, respectively; wherein the ultra-high performance liquid chromatography analysis conditions include: mobile phase A is acetonitrile, mobile phase B is aqueous phosphoric acid, and a gradient elution procedure is employed. The characteristic spectrum constructed by the method described in the present invention is rich in information, highly characteristic, and has good reproducibility.

The invention discloses application of neochlorogenic acid in preparation of a medicine for preventing and / or treating hand-foot-and-mouth disease caused by A16 type coxsackie virus (CVA16) infection, and belongs to the technical field of biological medicine. Researches prove that the neochlorogenic acid has the effect of resisting CVA16 infection on HEK293T cells and H4 cells, after the two cells treated by the neochlorogenic acid are infected by the CVA16, the viral genome RNA abundance is obviously reduced, and the cell lesion death condition is obviously relieved; meanwhile, a cytotoxicity test shows that the neochlorogenic acid has no obvious cytotoxicity to HEK293T cells and only has weak cytotoxicity to H4 cells. Therefore, in a reasonable dosage range, the neochlorogenic acid, which is a low-toxicity natural small molecule compound, can be used for preparing the anti-CVA16 medicine for preventing and / or treating the hand-foot-and-mouth disease caused by CVA16 infection.

The present application relates to a kind of Huahong composition effective part extract and its preparation method, the Huahong composition effective part extract includes the following components: syringic acid, new chlorogenic acid, deacetyl carlina acid methyl ester, geniposidic acid, N-acetyl-L-tyrosine, fraxidin, 4-pyridoxinic acid, 7,8-dihydroxy-4-methyl coumarin, chlorogenic acid, ferulic acid;The total content of above components is 80%-95% of effective part extract.The Huahong composition effective part extract of the present application is from the effective part of Huahong composition.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

Provided herein are polyphenol-enriched lettuce powders comprising particles of a polyphenol-enriched lettuce or extract, wherein the polyphenol-enriched lettuce comprises from 40-280 mg / g of polyphenols. The polyphenols include any combination of a chlorogenic acid, a neochlorogenic acid, a chicoric acid, a quercetin, a quercetin derivative, and an anthocyanin. The disclosure also provides compositions and methods of using the polyphenol-enriched lettuce powders to promote wound healing and treat inflammation and skin disorders.

The invention belongs to the technical field of medicinal material quality control, and discloses a honeysuckle flower QAMS (quantitative analysis of multi-components by single marker) quality control method, which comprises the following steps: preparing a single reference solution and a mixed reference solution of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C, and preparing a test solution; after high performance liquid chromatography detection, the relative correction factors of other components in the honeysuckle are calculated by taking chlorogenic acid as an internal reference substance through chromatographic peak positioning, and the contents of the components in the honeysuckle are obtained by utilizing the relative correction components, so that the synchronous determination of the multiple components in the honeysuckle is realized. The method has the advantages of cost saving, simplicity in operation, high detection accuracy and good stability, has great significance in quality control and clinical curative effect guarantee of the honeysuckle medicinal material, and provides a basis for establishing a quality evaluation standard of the honeysuckle medicinal material.

The present application relates to the technical field of traditional Chinese medicine detection, and provides a genuine three-leafed knot thin-layer identification kit and method, comprising: a chrysophanol control solution, a neochlorogenic acid control solution and a rapid extraction solvent, the rapid extraction solvent contains a low-carbon alcohol, which is used for extracting flavonoid glycoside components in three-leafed knot stem and leaf samples, and the kit is used for identifying the origin of three-leafed knot by detecting the content of chrysophanol and the presence of neochlorogenic acid. The present application uses a chrysophanol and neochlorogenic acid double-index mutual exclusion identification logic, combined with a rapid extraction solvent system without drying, to realize on-site, rapid and accurate identification of the genuineness of three-leafed knot, and effectively solves the problem of origin confusion.

The application discloses application of a medicine composition in preparation of a medicine for treating rheumatoid arthritis (RA), and belongs to the field of RA treating medicines. The medicine composition is composed of swertiamarin (SW) and neochlorogenic acid (NCA) and is screened from two active ingredients in Danteng Yimu Decoction. With the help of animal model and cell model experiments, the action mechanism of the medicine composition in treating RA is explored, the related symptoms and indexes of collagen-induced arthritis (CIA) rats can be effectively improved, joint swelling can be relieved, arthritis inflammation index can be reduced, inflammatory factor release can be inhibited, joint bone quality can be protected, arthritis inflammation infiltration and cartilage destruction can be reduced, and the medicine safety is good. The composition can effectively inhibit the inflammatory factor release and migration ability of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). Animal experiments and cell experiments prove that the combination of neochlorogenic acid and swertiamarin (SW+NCA) can be effectively applied to clinical treatment of RA and has a wide application prospect.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The present invention discloses a method for constructing a characteristic spectrum of a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction, comprising the following steps: preparing a reference solution, the reference solution comprising a control medicinal material reference solution and a reference substance reference solution, the reference substance reference solution comprising a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance, and a chlorogenic acid reference substance; taking a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction to prepare a test solution; taking a preset amount of the reference solution and the test solution, injecting them into a liquid chromatograph, the liquid chromatograph using octadecylsilane bonded silica gel as a filler, methanol as mobile phase A, and phosphoric acid solution as mobile phase B for gradient elution to establish a characteristic spectrum. The present invention can provide a data basis for the quality control of Corydalis guanzhong medicinal materials, formula granules, decoction pieces or standard decoctions, and ensure the stability and controllability of product quality.

The invention belongs to the technical field of traditional Chinese medicine component detection and analysis, and particularly relates to a method for detecting various active components in lonicerae flos by quantitative analysis of multi-components by single marker. According to the QAMS detection method for the various active ingredients in the lonicera confusa, based on a simple, convenient and rapid high performance liquid chromatography technology, QAMS of the active ingredients such as flavonoids, flavonoid glycosides, iridoid glycosides and organic acids in the lonicera confusa can be achieved, and qualitative and quantitative detection of the various active ingredients is included. The detection target objects comprise 14 active components, namely neochlorogenic acid, cumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, swertiamarin, secologanin, rutin, quercitrin, galuteolin, ferulic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C. The invention further provides a method for detecting the content of the isochlorogenic acid. Tests prove that the method disclosed by the invention meets methodological requirements, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, accuracy in determination and strong applicability, is beneficial to accurately evaluating the quality of the lonicerae flos and provides a reliable basis for safe application of the lonicerae flos.

The invention discloses a quality marker of cortex acanthopanacis semticosi and a preparation thereof and application of the quality marker in quality detection of cortex acanthopanacis semticosi, and belongs to the technical field of traditional Chinese medicine markers. The invention provides a quality marker of acanthopanax trifoliatus and a preparation thereof. The quality marker comprises neochlorogenic acid, chlorogenic acid and caffeic acid. According to the invention, the fingerprint spectrum of the cortex acanthopanacis semticosi standard decoction is firstly established, and then the change of the chemical components in the fingerprint spectrum is associated with the drug effect, so that the effective active component group of the cortex acanthopanacis semticosi standard decoction is screened out, the quality marker of the effective active component group is disclosed, and theoretical support is provided for deeply exploring the drug effect substances of the cortex acanthopanacis semticosi and carrying out quality research; and meanwhile, a scientific basis is provided for the quality improvement research of subsequent cortex acanthopanacis semticosi standard decoction and formula granules.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

The invention discloses an UPLC (Ultra Performance Liquid Chromatography) identification method for phenolic acid medicinal materials and formula granules, which is characterized in that the number of characteristic peaks of the phenolic acid medicinal materials and the formula granules is determined by a high performance liquid chromatography and is identified by a reference substance. The method can effectively identify medicinal materials and formula granules containing phenolic acid components. The preparation method comprises the following steps: preparing chlorogenic acid; chlorogenic acid; cryptochlorogenic acid; isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid A; isochlorogenic acid C; rutin; linarin; by analyzing the characteristic chromatograms of the traditional Chinese medicinal materials and formula granules and identifying the traditional Chinese medicinal materials and formula granules containing the components, a traditional Chinese medicinal preparation standard containing phenolic acid components is established, so that the detection and analysis of samples are facilitated, and the detection time and cost are saved.

The present invention discloses a method for simultaneously determining multiple chemical components in Cymbidium heterophyllum, and relates to the technical field of separation and analysis of natural products. Ultra-high performance liquid chromatography is used to simultaneously determine the chemical components of Cymbidium heterophyllum, and the chemical components include at least neochlorogenic acid, cryptochlorogenic acid, and diosgenin-7-O-glucuronide; the detection conditions of ultra-high performance liquid chromatography include: chromatographic column stationary phase: diisobutyl group is bonded to the silica gel surface through siloxane; mobile phase A is selected from methanol or acid-containing methanol, and mobile phase B is selected from water or acid-containing aqueous solution; mobile phase A and mobile phase B are used as a mixed mobile phase for gradient elution. The present invention establishes a UPLC content determination method for multiple chemical components in Cymbidium heterophyllum, which is simple to operate, has reliable results, and good repeatability, and can provide a certain reference for the determination of the content of chemical components in Cymbidium heterophyllum and the improvement of existing quality standards.

The invention discloses a preparation method of an acanthopanax trifoliatus granular preparation based on a quality marker, and belongs to the technical field of traditional Chinese medicines. The preparation method of the cortex acanthopanacis semticosi granular preparation is researched by taking the extract yield of cortex acanthopanacis semticosi, the content of effective components and the consistency of characteristic chromatograms as investigation indexes, and the extraction process parameters of the cortex acanthopanacis semticosi granular preparation are optimized by taking quality markers of neochlorogenic acid, chlorogenic acid and caffeic acid as evaluation index components and utilizing a Box-Behnken response surface method, so that the cortex acanthopanacis semticosi granular preparation is obtained. Meanwhile, a concentration process, a drying mode and a forming process are respectively investigated, the preparation method of the cortex acanthopanacis semticosi granular preparation is determined, and a scientific basis is provided for commercialized production of cortex acanthopanacis semticosi formula granules.

The invention discloses a quality control method for a standard sample of a five-ingredient disinfection drink. The quality control method comprises the following steps: (1) establishing a thin layer chromatography to identify honeysuckle, dandelion, herba violae and wild chrysanthemum in the standard sample; (2) establishing a fingerprint spectrum with relatively good separation degree and reproducibility by adopting a high performance liquid chromatography; (3) quantitatively researching 12 index components including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and the like through high performance liquid chromatography, and establishing a thin-layer identification method for honeysuckle, dandelion, philippine violet herb and wild chrysanthemum flower in the standard sample of the five-flavor disinfection drink; a method for determining the content of 12 index components such as neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid is established, a reference sample characteristic spectrum method is established, and the similarity of 15 batches of five-ingredient disinfection drink reference samples reaches 0.90 or above.

The present invention provides a method for establishing a fingerprint of a gypsophila preparation, comprising the following steps: S1, preparation of a test solution: weighing the gypsophila preparation, adding methanol, ultrasonically treating, filtering, and obtaining a test solution; S2, preparation of a reference solution: weighing eight reference substances, including cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, polydatin, astilbin, oxidized resveratrol, quercetin, and resveratrol, mixing, adding methanol, and obtaining a reference solution; S3, establishment of a fingerprint: respectively measuring the test solution and the reference solution prepared above by HPLC, recording the chromatograms and comparing them, identifying the common peaks, and obtaining a fingerprint of the gypsophila preparation. The invention successfully established a fingerprint of the gypsophila preparation by optimizing the extraction conditions and HPLC chromatographic conditions of the gypsophila preparation. The fingerprint establishment method is accurate, reliable, and simple to operate, and the detection method has high precision, good specificity, stability, and repeatability.

This invention discloses a method for constructing a fingerprint spectrum of a throat-clearing hard candy and a method for detecting its component content, belonging to the field of component detection technology. In this invention, the throat-clearing hard candy sample solution is analyzed by high-performance liquid chromatography (HPLC) to obtain a fingerprint spectrum of the candy; and the content information of the components is obtained through the external standard method, realizing qualitative and quantitative analysis of the components of the throat-clearing hard candy. Specifically, it involves the simultaneous qualitative and quantitative analysis of the following 10 components: gallic acid, neochlorogenic acid, chlorogenic acid, isorhamnetin-3-O-glucose-7-O-rhamnoside, rutin, luteolin, isochlorogenic acid B, isorhamnetin-3-O-glucose, glycyrrhizic acid, and kaempferol, providing a reliable and quantifiable standard for the quality assessment of throat-clearing hard candy. The chromatogram obtained by the method of this invention has the characteristics of stable baseline, high peak resolution, strong specificity, and good peak symmetry.

The present invention relates to a method for establishing a fingerprint of Hyssopus officinalis, and specifically to a method for determining the fingerprint of 18 characteristic peaks of Hyssopus officinalis, including neochlorogenic acid, chlorogenic acid, diosmin, rosmarinic acid, salvianolic acid B, and montanin, by using an HPLC method. In the method for establishing the fingerprint of the present invention, 328 nm is selected as the measurement wavelength; acetonitrile-0.1% phosphoric acid is used as the mobile phase and gradient elution is performed; preferably 70% methanol is used as the extraction solvent and the extraction method is heated under reflux for 40 minutes. The established method can accurately reflect the overall chemical characteristics of Hyssopus officinalis, is simple and quick, and provides a scientific basis for comprehensively improving its quality.

The present application belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a method for quantitatively analyzing effective components of Xiaochaihu medicinal materials. The method comprises the following steps: a, preparation of a control solution: precisely take new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, 3,5-O-dicaffeoylquinic acid, quercitrin and 4,5-O-dicaffeoylquinic acid control samples, and add methanol to prepare a mixed control solution; b, preparation of a test solution: crush Xiaochaihu medicinal materials, add a methanol solution for extraction, and filter to obtain the test solution; and c, determination: precisely take the control solution and the test solution, inject them into an ultra-high performance liquid chromatograph, and determine the content by using an external standard method. The method can simultaneously detect and analyze the content of seven chemical components in Xiaochaihu medicinal materials, has good linearity, repeatability, stability and recovery rate, and can effectively control the quality of Xiaochaihu medicinal materials.

The invention discloses preparation of a folium cortex eucommiae extract and application of the folium cortex eucommiae extract in improvement of age-related macular degeneration, and belongs to the technical field of traditional Chinese medicine extraction. The technical problem to be solved is how to prepare the folium cortex eucommiae extract which is higher in content of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, aucubin and geniposidic acid and has a better anti-age-related macular degeneration effect. According to the technical scheme, the folium cortex eucommiae extract is characterized in that the content of chlorogenic acid is 50-100 mg / g, the content of cryptochlorogenic acid is 40-60 mg / g, the content of neochlorogenic acid is 35-55 mg / g, the content of aucubin is 56-79 mg / g, and the content of geniposidic acid is 65-105 mg / g. The folium cortex eucommiae extract obtained by the method can better retain effective components in the folium cortex eucommiae extract, and a better anti-age-related macular degeneration effect is embodied.

The present application relates to the technical field of cultivation, in particular to a preparation method of astringent horehound extract, a feed additive and application thereof. The present application takes astringent horehound as raw material, the plant can be planted in large area, and fresh grass yield per mu can reach about 10 tons, and dry grass can reach more than 1 ton. The natural source feed additive active component (i.e. astringent horehound extract) is obtained by ethanol extraction combined with column chromatography. The content of chlorogenic acid in the component can reach more than 12.9%, and the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid can reach more than 35%. The active component as a feed additive shows good disease resistance in the feeding of Tibetan sheep, Ippy rabbits and small crayfish, and the yield of the animals is greatly improved, and the effect is better than that of the same dose of chlorogenic acid adding group. In addition, the astringent horehound extract prepared by the preparation method can significantly improve the meat quality and increase the yield.