42 results about "Isofraxidin" patented technology

The invention discloses a quality control method for traditional Chinese medicine oral liquid for treating psoriasis. The medicine is prepared from seven types of medicinal materials such as radix paeoniae rubra, rhizoma smilacis glabrae and glabrous sarcandra herbs. According to the specific quality control method, the thin layer chromatography is applied for conducting thin-layer identification on radix paeoniae rubra and rhizoma smilacis glabrae in PSORI-CMO1, meanwhile, the high performance liquid chromatography is combined for measuring the contents of chlorogenic acid, paeoniflorin, isofraxidin, astilbin and engeletin in the oral liquid, it is guaranteed that detected constituents are accurate and reliable, and the purpose of effectively controlling quality of the traditional Chinese medicine oral liquid can be achieved. By means of the quality control method, the defect that a method for detecting multiple constituents of compound traditional Chinese medicine is complex is overcome; the method has the advantages that by identifying the two types of medicine materials and measuring the contents of important active constituents in the medicine, inherent quality, the treatment effect and stability of a preparation are effectively guaranteed, and controllability of the quality standards for the PSORI-CMO1 oral liquid is improved.

The invention discloses a multi-index-based evaluating-optimizing glabrous sarcandra herb preparation technology. The multi-index-based evaluating-optimizing glabrous sarcandra herb preparation technology comprises the following steps of HPLC determination of specified components, process optimization of glabrous sarcandra herb leaves, single factor experimental investigation of glabrous sarcandraherb stems, cutting-preparing design scheme of the glabrous sarcandra herb stems, establishment of an AHP model, a comprehensive scoring method and CCD-RSM optimized glabrous sarcandra herb stem cutting-preparing process and verification test. Through a single-factor test, the comprehensive scores of the content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isofraxidin, astilbin and rosmarinic acid are taken as the evaluation indicators, a central composite design-response surface methodology is used to screen glabrous sarcandra herb softening and cutting-preparing processes, the glabrous sarcandra herb stems and leaves subjected to process optimization by choosing different treatment methods, a multi-index analytic hierarchy process (AHP) is combined with the central composite design-response surface methodology to optimize the glabrous sarcandra herb preparation technology, the study is beneficial to the further development of glabrous sarcandra herb, and an experimental basis is expected to be provided for the establishment of related preparation specifications and quality standards of glabrous sarcandra herb.

The invention provides a sarcandra glabra extract with anti-influenza virus activity. Counted by the total weight of the sarcandra glabra extract, the sarcandra glabra extract comprises 0.020-0.040wt%of chlorogenic acid, 0.000-0.008wt% of isofraxidin, 0.025-0.060wt% of quercetin-3-O-beta-D-glucuronide and 0.083-0.158wt% of rosmarinic acid. The invention further provides a preparation method of the sarcandra glabra extract with the anti-influenza virus activity. The preparation method comprises the following steps: performing reflux extraction on sarcandra glabra for two times with deionized water, performing alcohol precipitation to obtain a dry sarcandra glabra water extract, then preparing the dry extract into a sample with a certain concentration, injecting the sample into macroporousadsorption resin, then eluting with an ethanol solution with the concentration of 20%, and collecting an eluate to obtain the sarcandra glabra extract with the anti-influenza virus activity. The sarcandra glabra extract and the preparation method have the advantages as follows: a help is provided for application of the sarcandra glabra extract to fighting against influenza; by the preparation method, the steps are simple and convenient, the operation is easy, quantification can be achieved, the repeatability is high; the preparation method is suitable for application of the extract to the large-scale industrial production process of a medicine in the later stage.

The invention provides a microbial agent for improving soil acidification and a preparation method thereof. The microbial agent is prepared from the following components: sodium acetate, yeast extract, phycocyanin, folium artemisiae argyi, glucose, ammonium sulfate, astragalus smicus, isofraxidin, calcium hydrophosphate, dolomite dust, paprika powder, protamine lactobacillus powder, agrobacterium bacterial powder and torulaspora delbrueckii bacterial powder, wherein the protamine lactobacillus powder has a living cell concentration of protamine lactobacillus of 8*10<8>CFU / g; the agrobacterium bacterial powder has a living cell concentration of agrobacterium of 4*10<8>CFU / g; and the torulaspora delbrueckii bacterial powder has a living cell concentration of torulaspora delbrueckii of 2*10<9>CFU / g. By adopting the microbial agent, plant root rot and main root nondevelopment caused by soil acidification can be remarkably improved.

The invention relates to a method for extracting isofraxidin, which comprises the following steps of: pulverizing acanthopanax root serving as a raw material, and adding 10 to 20 percent water for wetting; adding biological enzyme for enzymolysis in the normal state for 3 to 10 days, and after the enzymolysis, extracting through the reflux in 80 to 90 percent methyl alcohol; and concentrating extracting solution under reduced pressure, adding ethyl acetate for dissolving to remove aqueous layer, decolorizing through an activated carbon alumina mixed column to remove impurities, concentrating and crystallizing, recrystallizing through the reflux of chloroform and acetone, and drying crystals at the low temperature to obtain the isofraxidin. In the method for extracting the isofraxidin, the product yield is high, the operation is simple, the repeatability is good, and the enlargement production is easy to perform.

The invention belongs to the technical field of capsule preparation quality control, and particularly relates to a quality control method of a capsule preparation for treating gastric ulcer. The quality control method is characterized in that the contents of scopolin, dehydroevodiamine, scopoletin, isofraxidin, rutaecarpin, evocarpine and evodiamine in galangal gastric ulcer capsules are determined by a HPLC (High Performance Liquid Chromatography) method, thereby building a quality control method of a galangal gastric ulcer capsule preparation for treating gastric ulcer. The adopted quality control method is scientific and reasonable, has high accuracy and high reproducibility, can comprehensively and effectively control the quality of the galangal gastric ulcer capsule preparation for treating duodenal ulcer, and can ensure the clinical effect of the preparation.

The invention relates to a method for inspecting the quality of slender acanthopanax stilbenes powder. The method comprises qualitative analysis and quantitative determination for the slender acanthopanax stilbenes powder. Compared with a method for inspecting the quality of a lingqijia oral solution, the method adopts a high performance liquid chromatography for measuring the content of astragaloside in the slender acanthopanax stilbenes powder, and a measuring method for the total sugar content is also adopted, a thin layer chromatography is adopted for respectively inspecting the astragaloside and isofraxidin in the slender acanthopanax stilbenes powder. The method is good in specificity, repeatability and accuracy and is used for performing comprehensive evaluation on the quality of the slender acanthopanax stilbenes powder so that the quality of a product is effectively controlled and safe, the effective and stable clinical use can be guaranteed.

The invention provides a quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali). The method comprises the processes of performing qualitative analysis and quantitative determination on the wujiaqi oral liquid, wherein the process of qualitative analysis is characterized in that thin layer chromatography is used for respectively authenticating astragaloside and isofraxidin in the wujiaqi oral liquid; the process of quantitative determination is characterized in that high performance liquid chromatography and ultraviolet-visible spectrophotometry are respectively adopted to detect the astragaloside content and contents of total sugar in the Wujiaqi oral liquid. Compared with the quality inspection method of Linqijia oral liquid (Chinese patent medicine prepared from lucid ganoderma, radix astragalus, acanthopanax root and saccharose), the inspection method disclosed by the invention is good in specificity, repeatability and accuracy, and can be used for comprehensively evaluating the quality of the Wujiaqi oral liquid, thereby effectively controlling the quality of the product, and guaranteeing the security, effectiveness and stabilization of the oral liquid in clinical use.

The invention provides a living cell vitrification freezing fluid and a freezing thawing method. The freezing fluid is prepared by that on the basis of an MEM culture medium and an F12 culture mediumin a volume ratio of 1:1, 50-60nmol / L of isofraxidin, 20-30nmol / L of chlorogenic acid and 40-48Mumol / L of a protective agent are added, wherein the protective agent comprises one or more of hydroxyethyl starch, tea polyphenol, 4-hydroxyethyl piperazine ethanesulfonic acid, lentinan and adenosine triphosphate. The living cell vitrification freezing fluid has the advantage that the living cell survival rate, the in-vitro fertilization and development rate, the cleavage rate and the blastocyst rate can be increased.

Vaccines containing adjuvant comprising eleutherosides and related compounds are shown to be useful for the prevention of viral infections, bacterial infections and parasitic infections. The adjuvant compounds have been shown to modulate the expression of a wide variety or proteins involved in the immune response and inflammatory response. Exemplary eleutherosides and related compounds include eleutheroside A, eleutheroside B, eleutheroside C, eleutheroside D, eleutheroside E, eleutheroside F, and eleutheroside G, coniferylaldehyde, caffeic acid ethyl ester, chlorogenic acid, sinapinalcohol, isofraxidin, syringaresinol and 6,8-dimethoxy-7-hydroxycoumarin.

The invention relates to a method for determining the content of compound Caoshanhu lozenge prepared from glabrous sarcandra herb extractum, menthol and dementholised peppermint oil. The thin-layer chromatography is used for distinguishing the menthol and the glabrous sarcandra herb extractum in the lozenge. The method is characterized in that the high performance liquid chromatography is used forsimultaneously determining the content of two marker components, including isofraxidin and rosmarinic acid in the lozenge under the chromatographic condition of isocratic elution; or the high perform ance liquid chromatography is used for simultaneously testing the content of four marker components, including protocatechuic acid, caffeic acid, the isofraxidin and the rosmarinic acid in the lozengeunder the chromatographic condition of gradient elution.

The invention relates to an isofraxidin crystalline compound and glabrous sarcandra herb dispersible tablets and dropping pills containing the isofraxidin crystalline compound. X-ray powder diffraction spectrogram characteristic peaks of the isofraxidin crystalline compound are measured by Cu-Ka rays and displayed at positions with 2theta being 6.8 degrees, 11.4 degrees, 12.0 degrees, 12.6 degrees, 14.8 degrees, 15.8 degrees, 18.0 degrees, 19.4 degrees, 20.1 degrees, 21.7 degrees, 23.4 degrees, 25.6 degrees, 28.0 degrees, 29.0 degrees and 30.6 degrees. Stability, bioavailability and quality of the glabrous sarcandra herb dispersible tablets and dropping pills are evidently improved, so that medication safety is guaranteed for patients. In addition, the glabrous sarcandra herb dispersible tablets and dropping pills have the advantages of stability in quality, accuracy in dosage, small difference in purity, easiness in implementation of mass mechanical production, high yield and low cost.