Extractive of effective part of chloranthus glaber and preparation method thereof

Abstract

The invention relates to a phenolic acid effective part extracted from the traditional Chinese medicine-chloranthus glaber and preparation method thereof. Phenolic acid compound contained in the effective part includes rosmarinic acid, protocatechuic acid, isofraxidin and caffeic acid, and the total content of the phenolic acid compound in the extractive is no less than 50 percent. The chloranthus glaber is extracted by water or ethanol, extracting solution is processed by high-speed centrifugation, centrifugation solution is processed by ultrafiltration by an organic composite film, the pH value of ultrafiltrate is regulated to range from 1 to 7, and the ultrafiltrate is applied to a resin column to be absorbed; according to column volume, 2 to 3 times water is used for elution, and then 10 to 70 percent of ethanol with the pH value ranging from 8 to 14 is used for elution; and ethanol elution solution is collected, concentrated and dried, thereby obtaining the extractive of the effective part.

Description

technical field [0001] The invention relates to an extract of effective parts of traditional Chinese medicine, in particular to an effective part of phenolic acids extracted from Chinese herbal coral coral, its preparation method and its application in the preparation of antibacterial and anti-inflammatory drugs and auxiliary therapeutic drugs for cancer. Background technique [0002] Grass coral (Sarcandra glabra (Thunb.) Nakai) is a plant of Chrysanthemum orchidaceae, also known as Sarcandra glabra, Jiujiecha, elderberry, elderberry, etc. Medicinal whole herb has the functions of antibacterial and anti-inflammatory, heat-clearing and detoxifying, expelling wind and dampness, promoting blood circulation and relieving pain, dredging menstrual flow and setting bones, etc. In recent years, it has also been used as an adjuvant therapy for cancer, and has obvious curative effects on pancreatic cancer, gastric cancer, rectal cancer, liver cancer, and esophageal cancer. [0003] ...

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Problems solved by technology

[0004] At present, most of the researches on the effective parts of Grass coral focus on flavonoids, coumarins and organic acid compounds, and there are very few studies on phenolic compounds. The patent with publication number CN 1879672A discloses a caffeic acid 3,4- The total polyphenols in the effective part of Sargassum with dihydroxyphenethyl ester as the active ingredient and the patent with the publication number of CN 101020637A disclose a method for preparing total polyphenols containing rosmarinic acid using Sargassum as a raw material, but the former The above-mentioned active ingredient caffeic acid 3,4-dihydroxyphenethyl ester has not been reported in the literature that it has been isolated from Grass coral, and the latter has no clear other phenolic components in the total polyphenols except rosmarinic acid, and The key lies in the fact that the preparation method of the effective parts adopted by the two is relatively cumbersome and difficult to implement, which is not convenient for industrialized mass production.

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