51 results about "Protocatechuic acid" patented technology

The invention provides an aluminum alloy pretreatment method based on complex reaction, which comprises the following steps: S1, polishing an LDH (Layered Double Hydroxides) growth surface by using abrasive paper, and cleaning and drying; s2, preparing a complexing agent solution, wherein a complexing agent is selected from at least one of tannic acid, gallic acid and protocatechuic acid; s3, the aluminum alloy sample treated in the step S1 is put into the complexing agent solution in the step S2, and constant potential is applied to the aluminum alloy; s4, preparing a mixed solution of Zn (NO3) 2.6 H2O and NH4NO3, and adding an ammonia water solution to adjust the pH value to 6-6.5; s5, immersing the aluminum alloy treated in the step S3 into the mixed solution in the step S4, and putting the aluminum alloy into a constant-temperature water bath kettle to generate a Zn-Al-LDH film; and S6, after the hydrothermal reaction is finished, cooling to room temperature, and washing and drying the obtained reaction product. The surface of the aluminum alloy is pretreated through the complexing agent, the potential between an intermetallic compound and an aluminum matrix can be effectively balanced, the micro-couple effect is fundamentally inhibited, and ideal conditions are created for uniform, compact and defect-free growth of LDH.

This invention discloses the application of reagents for detecting biomarker levels in the preparation of products for identifying obstructive sleep apnea. It relates to the field of biomarker technology and aims to overcome the shortcomings of existing technologies that rely on complex polysomnography (PSG) procedures, poor patient compliance, and a lack of effective molecular markers. The biomarkers used in this application consist of CD36 glycoprotein, 9-octadecenal, and protocatechuic acid 3-O-sulfate. This invention enables rapid and objective identification of obstructive sleep apnea by detecting the levels of the above biomarkers in plasma. The procedure is simple and well-accepted by patients. The combined use of multiple biomarkers can improve the accuracy and stability of the identification, making it suitable for screening or auxiliary diagnosis in medical institutions at different levels.

The invention relates to the technical field of medicines, and discloses a bifidobacterium longum strain GZBAI 01 and application thereof in degradation of benzoic acid and prevention or treatment of colorectal cancer. The strain is separated from faeces of healthy people, genomics identification is carried out, the metabolic profile of the strain is measured through metabonomics, the strain can convert benzoic acid with carcinogenic risk into protocatechuic acid with antitumor activity through unique metabolic capability, meanwhile, the function of regulatory T cells (Treg) in tumor drainage lymph nodes can be inhibited, differentiation of CD8 + T cells is promoted, and the tumor drainage lymph nodes can be inhibited. Therefore, the colorectal cancer is prevented through double ways of eliminating cancerogen and generating therapeutic agents. A colorectal cancer mouse model and a subcutaneous tumor mouse model verify that the strain can significantly reduce the number and volume of tumors, and shows a benzoic acid conversion effect superior to that of other strains in vivo and in vitro, and a new way is provided for prevention and treatment of colorectal cancer.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

Aspects of the present disclosure relate to the use of engineered host cells capable of producing protocatechuic acid (PCA). Aspects of the present disclosure also relate to compositions comprising engineered host cells capable of producing protocatechuic acid and / or cell constituents thereof, and the use of such compositions for altering and / or maintaining biological activity in a subject.

This invention discloses a mitochondrial-targeted nanomaterial for senescent cells and its application in anti-aging. The material is a nucleic acid aptamer-tetrahedral DNA, mainly assembled from four single-stranded DNA sequences, SEQ ID Nos. 1-4. Preparation involves first hybridizing the four single-stranded DNA sequences, then concentrating them to obtain aptamer-modified tetrahedra, which are then coupled with tannic acid (TA) via a Mann reaction in the presence of formaldehyde to obtain the Apt-tFNAs-TA complex. Finally, it is incubated with excess catechins to obtain the final delivery material, Apt-tFNAs@catechin-TA. This invention's material exhibits good biocompatibility and skin tissue compatibility, significantly improving uptake efficiency and mitochondrial localization ability in senescent cells. It can effectively deliver polyphenolic compounds such as catechins and protocatechuic acid, improving mitochondrial function and addressing the technical problems of low delivery efficiency of active ingredients and insufficient targeted enrichment in senescent cells.

The application discloses an aluminum profile spraying powder coating and a preparation method thereof, and belongs to the technical field of paint. The application introduces modified resin, so that the coating has excellent weather resistance, and adds specific proportion of proto-catechuic acid, 2-(4-thiazolyl) benzimidazole and 8-hydroxyquinoline as an antibacterial agent, and chemically bonds the antibacterial agent to the resin base material, so that efficient, long-lasting and broad-spectrum antibacterial effect is realized, and more significant antibacterial purpose is realized, and the problems of easy migration and easy failure of the surface spraying antibacterial agent are effectively solved. The application optimizes the overall formula, and the components interact with each other, so that the coating has excellent surface flatness and apparent quality, and the defects of pinholes and orange peel commonly existing in traditional coatings are avoided; the overall antibacterial and long-lasting antibacterial properties are significant, and the application is particularly suitable for buildings, medical treatment and household aluminum profiles with high sanitary requirements.

The application provides an extraction method of a vine tea extract and the extract. Primarily extracted is protocatechuic acid, and the extraction method mainly comprises a processing step, wherein the vine tea is steamed at 110-120 DEG C for 0.5-1.5 h, and then cut into segments for standby. The application also provides an application of the extract, which is used for preparing antioxidant and anti-inflammatory drugs and / or food and / or cosmetics. The application has the advantages that the extraction efficiency of protocatechuic acid is effectively enhanced through the synergistic effect of processing and extraction processes, the extraction rate is greatly increased from 0.1-0.6 mg / g in the prior art to 1-1.4 mg / g, and the increase is significant.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

The invention discloses protocatechuic acid O-methyltransferase based on artificial intelligence design and a design method of the protocatechuic acid O-methyltransferase, the amino acid sequence of the protocatechuic acid O-methyltransferase is SEQ ID NO: 1 or SEQ ID NO: 2, the active center of the protocatechuic acid O-methyltransferase comprises an octahedral coordination configuration formed by coordination of Mg < 2 + > and six coordination atoms, the protocatechuic acid O-methyltransferase is completed through artificial intelligence design; the similarity between the amino acid sequence of the obtained protocatechuic acid O-methyltransferase and the amino acid sequence BLAST of natural protocatechuic acid O-methyltransferase is relatively low, and the designed protocatechuic acid O-methyltransferase can be used for remarkably improving the enzymatic activity and the purity of a target product.

The invention provides a medicament for treating a milk disease of eriocheir sinensis and a preparation method of the medicament. The medicament is prepared from the following raw materials in parts by weight: 6-7 parts of traditional Chinese medicine components, 2 parts of allicin and 1 part of an additive, wherein the traditional Chinese medicine components comprise coptis chinensis, golden cypress, schisandra chinensis, astragalus membranaceus and gallnut, and the additives comprise epsilon-polylysine, protocatechuic acid and rosmarinol. According to the medicament for treating the milk disease of the eriocheir sinensis, the efficacy limitation of a single natural medicine is broken through through a technology of combining the compound traditional Chinese medicine components with the allicin and the additive, the application of the natural medicine of aquatic products is promoted, a culture mode is assisted to be transformed to be green and efficient, and a brand new thought is provided for treating the milk disease.

The present application belongs to the field of food science and technology, and particularly relates to a method for separating and purifying walnut male flower polyphenols based on macroporous adsorption resin and application. Research shows that the X-5 type resin has the best performance in adsorbing and desorbing walnut male flower polyphenols, the adsorption process conforms to the Temkin isothermal model and the pseudo-second-order kinetic model, indicating that it is a spontaneous exothermic physical adsorption process. After process optimization (80% ethanol, 2 BV / h flow rate, 10 BV elution volume), the polyphenol content is significantly improved. The purified product detects 26 kinds of phenolic acid compounds and 22 kinds of flavonoids, the main components are astragalin, isquercitrin, protocatechuic acid, gallic acid and quercetin, accounting for 89.91% of the total phenol content. The antioxidant activity of the purified polyphenol extract is significantly enhanced, and it has obvious bacteriostatic effect on Staphylococcus aureus. The present application provides a theoretical basis for the high-value utilization of walnut male flower resources and its application in the fields of food and health care.

The application provides a construction method of UPLC characteristic spectrum of Qinglongyi medicinal material, standard decoction and formula granules, comprising the following steps: dissolving and extracting raw materials of the test sample by using a solvent to obtain a test solution; determining the test solution by using high performance liquid chromatography to obtain the UPLC characteristic spectrum of Qinglongyi medicinal material, standard decoction and formula granules; and the high performance liquid chromatography conditions are as follows: a C 18 column is used as the chromatographic column; acetonitrile solution is used as mobile phase A, and 0.1% formic acid aqueous solution is used as mobile phase B, and gradient elution is adopted. The application adopts the high performance liquid chromatography, selects acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution, takes protocatechuic acid, vanillic acid, syringic acid, ellagic acid, rutin and quercitrin as the reference substances, establishes the UPLC characteristic spectrum method of Qinglongyi medicinal material, standard decoction and formula granules, and provides more scientific technical means for controlling the medicinal quality of Qinglongyi medicinal material, standard decoction and formula granules.

The present application belongs to the technical field of medicine, and relates to a kind of pharmaceutical composition and its application in preparation for treating or preventing liver fibrosis drug. A kind of pharmaceutical composition for treating or preventing liver fibrosis, the pharmaceutical composition includes as active ingredient the protocatechuic acid (PCA) and gimalin (GER). The present application first confirms that protocatechuic acid and gimalin combined application has significant synergistic effect in the treatment of liver fibrosis, and its curative effect is better than any single compound single administration, can more effectively reduce liver injury index, improve liver pathological morphology, reduce intrahepatic collagen deposition. Based on its clear mechanism of action, the composition of the present application is especially suitable for treating liver fibrosis related to abnormal expression of RIPK3.

The present disclosure relates to an extraction method capable of increasing effective components such as polyphenols and flavonoids using a high-temperature and pressurization steam extraction process. The high-temperature and pressurization steam extraction process of the present disclosure can significantly increase the extractive contents of protocatechuic acid and chlorogenic acid, as well as gallic acid, which is a powerful antioxidant as an effective component. In addition, the extractive contents of catechin and tannic acid, which are effective in inhibiting wrinkle formation, can also be effectively increased. The extract extracted through this extraction method contains a high content of polyphenol and flavonoid components, and therefore, the composition of the present disclosure containing the extract as an effective component can be usefully used as a variety of functional cosmetic compositions such as antioxidant, wrinkle improvement, and whitening components.

This invention discloses a genetically engineered bacterium for synthesizing protocatechuic acid and its applications, belonging to the field of gene recombination technology. The genetically engineered bacterium for synthesizing protocatechuic acid disclosed in this invention is a recombinant *Corynebacterium glutamicum* strain based on *Corynebacterium glutamicum* ATCC13032, or a *Corynebacterium glutamicum* strain modified with a chassis microbial substrate, including the shikimic acid pathway; and / or the DAHP pathway; and / or the 4-HBA pathway. The highest concentration of protocatechuic acid obtained by fermentation of the recombinant *Corynebacterium glutamicum* constructed in this invention can reach 85.16 g / L, which is the highest yield of protocatechuic acid synthesized de novo by biological methods reported in related studies to date. This has great potential for the industrial application of high-yield protocatechuic acid by biological methods. The genetically engineered bacterium constructed in this invention is safe and non-toxic, and can efficiently produce protocatechuic acid using glucose as a substrate through microbial fermentation. Furthermore, the culture medium composition is simple, batches are stable, and production costs are low.

The invention relates to an asymmetric adhesion bionic hydrogel dressing and a preparation method thereof. The preparation method is characterized by comprising the following steps: (1) dissolving acrylamide and acrylic acid in water; then adding N, N '-methylene bisacrylamide and N, N, N', N '-tetramethylethylenediamine, then adding FeCl3 and ammonium persulfate, and carrying out crosslinking to obtain upper-layer hydrogel; (2) adding protocatechuic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide into the hydroxypropyl chitosan solution, so as to obtain an adhesive layer; and (3) coating one surface of the upper-layer hydrogel with the adhesion layer, and curing at room temperature to obtain the asymmetric adhesion bionic hydrogel dressing. The asymmetric adhesion bionic hydrogel dressing has the advantages that one surface of the prepared asymmetric adhesion bionic hydrogel dressing has good adhesion performance and is used for being adhered to a wound, and the other surface of the asymmetric adhesion bionic hydrogel dressing is low in adhesion performance; the adhesive composition can be firmly adhered to tissues and can prevent the generation of bad adhesion.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

The application discloses a kind of different ecological region's Bunge grass seed field planting method, belong to pasture seed production technical field.The method is aimed at the environment difference of temperate saline-alkali prone area and temperate typical grassland area, based on the allelopathic effect of acid substance to Bunge grass and physiological and biochemical interaction law, and optimally constructs two kinds of composite acid initiation system of ferulic acid+salicylic acid+protocatechuic acid saline-alkali coordination type and chlorogenic acid+hydroxybenzoic acid+coumaric acid grassland coordination type.The application makes the emergence rate to be increased to 85% or more, seed yield is increased by 20%-30% compared with conventional extensive planting, standard germination rate reaches 90% or more, neatness is 75% or more, large-diameter seed accounts for 60% or more, small-diameter and shriveled grain accounts for 10% or less, and the coefficient of variation of grain size is low, which provides green and efficient technical support for native grass seed breeding.

The invention provides a postharvest fruit preservative and fresh-keeping composite bacteriostatic agent and a preparation method and application thereof, and belongs to the technical field of postharvest fruit fresh-keeping, the composite bacteriostatic agent is composed of at least two of ethyl protocatechuate, sodium silicate, disodium ethylene diamine tetraacetate and tamarind fruit kernel extracting solution; the concentrations of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate in the composite bacteriostatic agent are not lower than the EC50 concentrations of the ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate to pathogenic bacteria, and the adding volume of the tamarind kernel extracting solution in the composite bacteriostatic agent is the same as the total volume of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate; the pathogenic bacteria are at least one of citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus colletotrichum gloeosporioides. The compound bacteriostatic agent disclosed by the invention can ensure food safety and avoid the problem of environmental pollution; the compound bacteriostatic agent has a remarkable and specific prevention and treatment effect on main postharvest citrus diseases caused by citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus anthrax, and can be used for developing a novel postharvest citrus preservative.

The invention relates to the technical field of food preservation, and particularly discloses a screening method and application of plant lactobacillus Q9 with an agaricus bisporus postharvest preservation function. And a screening step: separating suspected lactic acid bacteria from traditional fermented milk in Gannan in Qinghai-Tibet Plateau, carrying out Gram-positive and catalase-free activity identification, screening strains which grow well and are high in polyphenol conversion rate by taking agaricus bisporus characteristic polyphenols such as gallic acid and protocatechuic acid as substrates, and carrying out sensory evaluation to screen out the plant lactobacillus Q9. When the Q9 bacterial suspension is applied, the bacterial suspension containing 1.5 * 10 < 8 > CFU / mL of Q9 is sprayed on the surface of agaricus bisporus, browning can be inhibited, the weight loss rate and the respiration rate can be reduced, the growth of pseudomonas can be inhibited, the related enzyme activity can be regulated and controlled, the shelf life can be prolonged, and a safe and efficient biological fresh-keeping scheme is provided for the postharvest fresh-keeping of agaricus bisporus.

This invention provides a method for determining the content of lotus root nodes and related processed products by HPLC, comprising: A) dissolving and extracting the test sample raw material in a solvent to obtain a test solution; the test sample raw material includes lotus root nodes or related processed products; B) determining the test solution by high-performance liquid chromatography to obtain an HPLC chromatogram of the lotus root nodes or related processed products; the chromatographic conditions of the high-performance liquid chromatography are: a C18 column; mobile phase A is a methanol solution, mobile phase B is a 0.5% phosphoric acid aqueous solution, and gradient elution is performed; the gradient elution is specifically as follows: 0-1 min, phase A: 2%, phase B: 98%; 1-4 min, phase A... Phase A: 2% → 8%, Phase B: 98% → 92%; 4–6 min, Phase A: 8% → 0%, Phase B: 92% → 100%; 6–16 min, Phase A: 0%, Phase B: 100%; 16–30 min, Phase A: 10%, Phase B: 90%. This invention employs high-performance liquid chromatography (HPLC) with a methanol-0.5% phosphoric acid solution as the mobile phase for gradient elution. Using protocatechuic acid and p-hydroxybenzoic acid as reference standards, an HPLC method for determining the content of lotus root nodes and related processed products was established, providing more scientific technical means for controlling the medicinal quality of lotus root nodes and related processed products.

The invention discloses an aluminum profile spraying powder coating and a preparation method thereof, and belongs to the technical field of coatings. The modified resin is introduced, so that the coating has excellent weather resistance, protocatechuic acid, 2-(4-thiazolyl) benzimidazole and 8-hydroxyquinoline in a specific proportion are added and compounded to serve as an antibacterial agent, the antibacterial agent is chemically bonded into a resin base material, the efficient, lasting and broad-spectrum antibacterial effect is achieved, the more remarkable antibacterial purpose is achieved, and the coating has the good application prospect. The problems that an antibacterial agent sprayed on the surface is prone to migration and failure are effectively solved. According to the invention, the whole formula is optimized, and the components interact with one another, so that the coating has excellent surface flatness and apparent quality, and the common defects of pinholes and orange peels in the traditional coating are avoided; the antibacterial aluminum profile has remarkable antibacterial property and lasting antibacterial property on the whole, and is particularly suitable for buildings, medical treatment and home furnishing aluminum profiles with high sanitary requirements.

The invention discloses metal surface anticorrosive paint and a preparation method thereof, and relates to the field of metal anticorrosive paint. When the metal surface anticorrosive paint is prepared, modified castor oil and triethylamine hydrochloride react to prepare a castor oil-based cross-linking agent; the preparation method comprises the following steps: carrying out epoxidation reaction on hexafluorobisphenol A and epoxy chloropropane to prepare an epoxy prepolymer; reacting the epoxy prepolymer with protocatechuic acid to obtain modified epoxy resin; the preparation method comprises the following steps: reacting 2-aminobenzothiazole with isocyanate propyl triethoxy silane to prepare modified silane; and uniformly mixing the modified epoxy resin, the modified silane and the mixed solvent, and adding the flatting agent and the castor oil-based cross-linking agent to prepare the metal surface anticorrosive paint. The metal surface anticorrosive paint prepared by the invention has excellent corrosion resistance, antibacterial property and high adhesive force.

The invention discloses a high-temperature-resistant anti-aging electronic component coating and a preparation method thereof, and relates to the technical field of coatings. The high-temperature-resistant anti-aging electronic component coating prepared by the invention is prepared from the following raw materials in parts by weight: epoxy resin, modified soybean oil, a silane fluorenyl epoxy monomer, a curing agent, modified mica powder and acetone. The preparation method comprises the following steps: enabling soybean oil to react with 3-(dimethylamino)-1-propanethiol and epoxy chloropropane in sequence, and introducing quaternary ammonium salt and an epoxy group to the soybean oil; the preparation method comprises the following steps: reacting bisphenol fluorene with dichlorodiphenyl silane and epoxy chloropropane in sequence to obtain an epoxy monomer containing a siloxane structure and fluorenyl, and participating in curing of the coating; mica powder with excellent thermal stability is subjected to surface modification with kh550 and then reacts with protocatechuic acid, and the protocatechuic acid is loaded on the mica powder. Through the improvement, the coating is endowed with excellent high-temperature resistance and antibacterial ability.

The application belongs to the technical field of water treatment, and particularly relates to a modified agar, a preparation method thereof and a scale inhibitor. The modified agar is formed by free radical grafting of carboxyl agar and protocatechuic acid; the carboxyl agar comprises carboxyl groups, the carboxyl groups comprise carboxymethyl groups, and are used for chelation with metal cations in water to inhibit scale formation; and the protocatechuic acid comprises hydroxyl groups, the hydroxyl groups comprise phenolic hydroxyl groups, and are used for destroying the structural integrity of bacteria in water to inhibit biological pollution of water. The modified agar as the scale inhibitor can effectively alleviate the scaling problem in the water treatment process through chelation, dispersion and other effects, improve the separation performance of lithium and magnesium ions, and reduce biological pollution; and the raw materials used are agar and protocatechuic acid, both of which are derived from natural plants, so the modified agar has the characteristics of non-toxicity and no secondary pollution.

The present application relates to the field of traditional Chinese medicine analysis, in particular to the preparation method, detection method and characteristic spectrum construction method of small canthium extract. The preparation method comprises the following steps: decocting small canthium medicinal materials twice and filtering to obtain decoction, and concentrating and drying the decoction to obtain small canthium extract. The small canthium extract has high extract yield, and the mass content and transfer rate of protocatechuic acid are both high. The protocatechuic acid mass content and / or transfer rate detection method and the characteristic spectrum construction method provided by the present application have good specificity, good repeatability and good stability. The present application provides a standard for the quality control of small canthium extract, and realizes the overall quality control and effective supervision of small canthium extract and its preparations.

The application relates to application of protocatechuic acid as an inhibitor of a calcium ion channel, belongs to the field of medicine, and also provides use of protocatechuic acid in preparation of drugs for treating cardiovascular diseases, coronary heart disease, atherosclerosis, end-stage renal failure, nervous diseases, chronic pain, acute pain or inflammatory diseases, proves that the protocatechuic acid can inhibit the calcium ion channel, especially a TRPC ion channel, and expands the use of the protocatechuic acid.

This application provides the use of protocatechuic acid in the preparation of medicaments for the prevention and treatment of respiratory syncytial virus (RSV) infection, and also provides the use of an aqueous extract of Cyperus rotundus containing protocatechuic acid in the preparation of medicaments for the prevention and treatment of RSV infection, as well as pharmaceutical compositions for the prevention and / or treatment of RSV infection and their uses. The aqueous extract of Cyperus rotundus and its component protocatechuic acid provided in this application have anti-RSV activity, and can therefore be used to prevent and / or treat RSV infection, and further for the preparation of medicaments for the prevention and / or treatment of RSV infection.