235 results about "Protocatechuic acid" patented technology

The invention relates to the field of sponge materials, and provides an antibacterial degradable sponge material and a preparation method thereof. The material is prepared from protocatechuic acid modified quaternized chitosan, quaternary ammonium salt grafted gelatin, sodium alginate, polyhydroxybutyrate, polycaprolactone, a polylactic acid-glycolic acid copolymer, hexamethylene diisocyanate, a foaming agent, nano cellulose, vitamin E, polyethylene glycol, triethylamine and dimethylformamide. Protocatechuic acid and quaternized chitosan are subjected to amidation modification, and chitosan and gelatin are subjected to quaternization treatment by adopting 2, 3-epoxypropyl trimethyl ammonium chloride, so that the antibacterial property and biodegradability of the material are improved. In the material preparation process, through vacuum degassing, foaming vacuum degree and humidity control and a multi-step drying process, it is ensured that the average pore size of the material is uniform (50-200 microns) and the porosity is high (50%-90%), and the obtained material has excellent antibacterial property and degradability and has wide application value.

The invention relates to the technical field of fermentation of edible and medicinal fungi, and discloses a phellinus igniarius fermentation method capable of producing high-yield Hispidin and an application of the phellinus igniarius fermentation method. The phellinus igniarius fermentation method comprises the following steps: inoculating a phellinus igniarius strain into a culture medium containing a copper ion compound, and fermenting. The phellinus igniarius mycelium obtained by adopting the fermentation method is rich in Hispidin, and can be widely used for preparing related medicines and health foods for protecting liver, resisting tumors, resisting viruses, resisting oxidation, reducing uric acid and the like. In addition, the invention also discloses a high performance liquid chromatography detection method for analyzing the content of Hispidin, by adopting the method, the content of Hispidin and precursor substances (such as protocatechuic acid, protocatechualdehyde, caffeic acid, osmundone and the like) in the phellinus igniarius leavening and derivative products thereof as well as phellinus igniarius mycelia can be effectively analyzed, and the accuracy of Hispidin detection is favorably improved.

A transformant produced by introducing a chorismate pyruvate-lyase gene comprising DNA (a), (b), (c), (d) or (e) and a 4-hydroxybenzoate hydroxylase gene comprising DNA (f), (g), (h), (i) or (j) into a Hydrogenophilus bacterium can utilize carbon dioxide as a sole carbon source to produce protocatechuic acid with high efficiency. (a) DNA that comprises the nucleotide sequence represented by SEQ ID NO:1; (b) DNA that comprises a nucleotide sequence having 90% or higher identity with SEQ ID NO:1 and encodes a polypeptide having a chorismate pyruvate-lyase activity; (c) DNA that encodes a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2; (d) DNA that encodes a polypeptide comprising an amino acid sequence having 90% or higher identity with SEQ ID NO:2 and having a chorismate pyruvate-lyase activity; (e) DNA that encodes a polypeptide comprising an amino acid sequence having the deletion, substitution, insertion or addition of 1 to 10 amino acid residues in the amino acid sequence represented by SEQ ID NO:2 and having a chorismate pyruvate-lyase activity; (f) DNA that comprises the nucleotide sequence represented by SEQ ID NO:3; (g) DNA that comprises a nucleotide sequence having 90% or higher identity with SEQ ID NO:3 and encodes a polypeptide having a 4-hydroxybenzoate hydroxylase activity; (h) DNA that encodes a polypeptide comprising the amino acid sequence represented by SEQ ID NO:4; (i) DNA that encodes a polypeptide comprising an amino acid sequence having 90% or higher identity with SEQ ID NO:4 and having a chorismate pyruvate-lyase activity; and (j) DNA that encodes a polypeptide comprising an amino acid sequence having the deletion, substitution, insertion or addition of 1 to 30 amino acid residues in the amino acid sequence represented by SEQ ID NO:4 and having a 4-hydroxybenzoate hydroxylase activity.

The invention provides a biological fermentation feed for enhancing immunity of aquatic animals and a preparation method of the biological fermentation feed, and relates to the technical field of aquaculture. The biological fermentation feed is prepared by compounding fish bone meal, a rice bran fermentation material, soybean meal, a compound fermentation material, visceral meal, orange peel powder, corn starch, compound vitamins, tea saponin and protocatechuic acid. According to the present invention, the defects in the prior art are overcome, the weight gain growth of the aquatic product is comprehensively ensured through the compound addition of the microbial components of the aquatic product feed and the regulation and control of other components, the immunity of the aquatic product is further improved, and the artificial breeding benefit of the aquatic product is comprehensively ensured.

The invention discloses benzoxazine resin based on protocatechuic acid and synthesis and application thereof.The protocatechuic acid serves as a raw material, amidation is conducted through furfuryl amine, and then a protocatechuic acid-based benzoxazine monomer (NDBA-fa) is synthesized with furfuryl amine and paraformaldehyde through a one-pot method; the resin monomer can be independently cured to form the protocatechuic acid benzoxazine resin. When the mass content of the NDBA-fa enhanced bisphenol A-aniline benzoxazine (BA-a) is 7.5%, the glass transition temperature Tg, the thermal decomposition temperature Td5% and the thermal decomposition temperature Td10% of the cured resin are respectively improved by 21 DEG C, 14 DEG C, 23 DEG C and 16.8%, and the heat release capacity HRC is reduced by 76.3 Jg <-1 > K <-1 >. The protocatechuic acid-based benzoxazine monomer provided by the invention can be used as a novel modifier, and can effectively improve the thermal stability and flame retardant property of BA-a. The application range of biological resources is also widened. # imgabs0 #

The invention belongs to the technical field of waterborne polyurethane, and discloses photo-thermal antibacterial, self-enhanced and self-healing vegetable oil-based waterborne polyurethane. The high-temperature-resistant polyurethane elastomer is prepared from a system consisting of the following raw materials in parts by weight: 80 to 120 parts of castor oil, 60 to 80 parts of dicyclohexylmethane diisocyanate, 20 to 40 parts of hexamethylene diisocyanate, 20 to 40 parts of N-methyldiethanolamine, 20 to 50 parts of protocatechuic acid, 2 to 10 parts of metal ions, 2000 to 3000 parts of water and 0.5 to 1 part of a catalyst. Metal ions in the photo-thermal antibacterial, self-enhanced and self-healing plant oil-based waterborne polyurethane and phenolic hydroxyl groups in protocatechuic acid are coordinated and self-assembled to form metal nanoclusters, and the waterborne polyurethane material has self-enhanced and self-healing properties due to a low-bond-energy coordination aggregation state structure; meanwhile, the waterborne polyurethane film is endowed with photo-thermal antibacterial performance by the metal nanoclusters. The invention further discloses a preparation method and application of the photo-thermal antibacterial, self-enhanced and self-healing plant oil-based waterborne polyurethane.

The invention discloses siloxane bridged protocatechuic acid-based epoxy resin as well as a synthesis method and application thereof. Carboxyl of protocatechuic acid is bridged with epoxy groups of polydimethylsiloxane with different chain segment lengths, and then phenolic hydroxyl of protocatechuic acid is subjected to epoxidation reaction through a two-step method, so that the siloxane bridged protocatechuic acid-based epoxy resin is obtained. A novel siloxane bridged protocatechuic acid-based epoxy resin monomer is synthesized, and the structural formula of the monomer is shown as a formula (III). The siloxane-bridged protocatechuic acid-based epoxy resin has the characteristics of low viscosity, high toughening property, low dielectric constant and strong hydrophobicity, the processability of the siloxane-bridged protocatechuic acid-based epoxy resin is improved, and the siloxane-bridged protocatechuic acid-based epoxy resin shows excellent fracture resistance. Through the synergistic improvement, the siloxane bridged protocatechuic acid-based epoxy resin obtained by the invention becomes novel high-performance thermosetting plastic, and has wide application prospects in the fields of electronic packaging and semiconductor industries and architectural coatings.

The invention belongs to the technical field of chemical drugs, and particularly relates to application of a polyphenol composition containing gallic acid to preparation of drugs for treating inflammatory bowel diseases and the drugs. The polyphenol composition contains caffeic acid, ferulic acid, gallic acid, hesperidin, isoquercitrin, isorhamnetin, kaempferol, protocatechuic acid and quercetin, and has an obvious anti-inflammatory effect on the inflammatory bowel disease; when the composition is used as a medicine for treating the inflammatory bowel disease, the treatment cost can be reduced, and the composition is effective in treating the inflammatory bowel disease and small in toxic and side effects after being taken for a long time.

The invention discloses a redness repairing and relieving composition as well as a preparation method and application thereof. The red repairing and relieving composition comprises a red repairing and relieving functional agent, wherein the components of the red repairing and relieving functional agent comprise a phenolic compound and a phenolic acid compound; the phenolic compound is selected from at least one of hydroxytyrosol and tyrosol; the phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, plateau catechuic acid and ethyl protocatechuate. The composition can inhibit release of inflammatory factors, relieve itching, resist inflammation, calm skin and reduce angiogenesis, and meanwhile can enhance the skin water locking capacity, improve the skin water shortage condition and enable the skin to recover to be moisturized, tender and smooth.

The invention provides an application of protocatechuic acid in an anti-inflammatory active pharmaceutical composition in dairy cow mammary epithelial cells, belongs to the technical field of bioengineering, and aims to obtain high-purity protocatechuic acid (greater than or equal to 95%) by optimizing a preparation process through the application of protocatechuic acid in inhibition of inflammatory response of dairy cow mammary epithelial cells. Under the concentration of 1-100 mu M, the level of inflammatory factors such as TNF-alpha, IL-6 and the like is remarkably reduced (the inhibition rate is larger than or equal to 60%), the mechanism is that NF-kB and MAPK signal channels are inhibited, cytotoxicity is avoided, and a milk region perfusion agent or a feed additive can be prepared to be used for preventing and treating dairy cow mastitis.

The invention discloses injectable hydrogel for treating periodontitis and a preparation method of the injectable hydrogel. The injectable hydrogel is prepared from protocatechuic acid grafted carboxymethyl chitosan hydrogel and a zinc-metformin complex loaded in the protocatechuic acid grafted carboxymethyl chitosan hydrogel. The hydrogel aims to become a safe and effective treatment method so as to reduce oxidative stress and tissue damage caused by periodontitis. And the hydrogel is designed into an injectable form, so that the clinical operation is facilitated, and the comfort of a patient is improved. Therefore, the treatment effect and life quality of patients with chronic periodontitis are improved, and inconvenience and discomfort in the treatment process are reduced.

The invention relates to the technical field of tissue engineering material preparation, and discloses a gelatin chitosan-based hydrogel preparation method and application thereof, and the gelatin chitosan-based hydrogel preparation method comprises the following steps: dissolving chitosan in an aqueous solution containing alpha-ketoglutaric acid and protocatechuic acid to prepare a chitosan solution; in the process, alpha-ketoglutaric acid reacts with amino of chitosan, and meanwhile, alpha-ketoglutaric acid and protocatechuic acid act synergistically to form a basic reaction system of the solution. The preparation method comprises the following steps: adding gelatin into a chitosan solution, so that chitosan, alpha-ketoglutaric acid, protocatechuic acid and gelatin in the solution are fully mixed through blending to form a uniform pre-reaction system; the mixed solution is placed under a proper temperature condition for incubation, in the process, a series of non-covalent bond effects and local chemical bond effects occur in the system, and the chitosan and the gelatin form a stable network structure. After incubation is completed, the solution is gradually converted into gel with excellent performance. And the finally prepared hydrogel has good biocompatibility and temperature-sensitive characteristics.

The invention discloses a construction method of a characteristic chromatogram of a rhizoma dryopteris crassirhizomae medicinal material, formula granules, decoction pieces or standard decoction, and the construction method comprises the following steps: preparing a reference substance solution which comprises a reference substance solution of a reference medicinal material and a reference substance solution of a reference substance, the reference substance solution comprises a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance and a chlorogenic acid reference substance; taking a rhizoma dryopteris crassirhizomae medicinal material, formula granules, decoction pieces or a standard decoction to prepare a test solution; a preset amount of the reference substance solution and the test solution are taken and injected into a liquid chromatograph, the liquid chromatograph takes octadecylsilane chemically bonded silica as a filler, methanol as a mobile phase A and a phosphoric acid solution as a mobile phase B for gradient elution, and the characteristic chromatogram is established. The method can provide a data basis for quality control of rhizoma dryopteris crassirhizomae medicinal materials, formula granules, decoction pieces or standard decoction, and ensures the stability and controllability of product quality.

The invention discloses an absorbable suture line containing fibroin and a preparation method of the absorbable suture line, and relates to the technical field of absorbable suture lines. The absorbable suture line containing the silk protein is prepared from the following raw materials in parts by weight: 35 to 50 parts of silk protein, 10 to 20 parts of polylactic acid, 15 to 25 parts of polyglycolide-lactide, 1 to 3 parts of antibacterial agent, 2 to 5 parts of modified toughening agent, 1 to 4 parts of nano mineral filler, 0.5 to 2 parts of cross-linking agent and 2 to 5 parts of plasticizer, the preparation method comprises the following steps: reacting protocatechuic acid with ethanolamine to generate an intermediate 1; and reacting the intermediate 1 with 5, 10, 15, 20-tetra (4-carboxyl phenyl) porphyrin to generate the antibacterial agent. The prepared absorbable suture line containing the silk protein has good mechanical performance, self-repairing performance and antibacterial performance.

The invention discloses a compound wintercreeper capsule high performance liquid chromatography fingerprint spectrum analysis method and application thereof, and belongs to the technical field of chemical analysis. A mixed solution of protocatechuic acid, calycosin glucoside, formononin, calycosin and formononetin is used as a standard solution; performing high performance liquid chromatography analysis by taking a methanol extracting solution of the compound wintercreeper capsule as a test solution, and establishing an HPLC fingerprint spectrum of the compound wintercreeper capsule; wherein in the high performance liquid chromatography analysis, an acetonitrile-0. 2% phosphoric acid solution is used as a mobile phase, the elution mode is gradient elution, and the detection wavelength is 254 nm. The method provided by the invention is good in repeatability and high in durability, and can be used for controlling the quality of the compound wintercreeper capsule.

The invention provides an aluminum alloy pretreatment method based on complex reaction, which comprises the following steps: S1, polishing an LDH (Layered Double Hydroxides) growth surface by using abrasive paper, and cleaning and drying; s2, preparing a complexing agent solution, wherein a complexing agent is selected from at least one of tannic acid, gallic acid and protocatechuic acid; s3, the aluminum alloy sample treated in the step S1 is put into the complexing agent solution in the step S2, and constant potential is applied to the aluminum alloy; s4, preparing a mixed solution of Zn (NO3) 2.6 H2O and NH4NO3, and adding an ammonia water solution to adjust the pH value to 6-6.5; s5, immersing the aluminum alloy treated in the step S3 into the mixed solution in the step S4, and putting the aluminum alloy into a constant-temperature water bath kettle to generate a Zn-Al-LDH film; and S6, after the hydrothermal reaction is finished, cooling to room temperature, and washing and drying the obtained reaction product. The surface of the aluminum alloy is pretreated through the complexing agent, the potential between an intermetallic compound and an aluminum matrix can be effectively balanced, the micro-couple effect is fundamentally inhibited, and ideal conditions are created for uniform, compact and defect-free growth of LDH.

The invention discloses a metal phenolic composite material with an antibacterial synergistic effect and a preparation method thereof, protocatechuic acid and zinc acetate are added according to a chemical reaction molar ratio, the protocatechuic acid and the zinc acetate can react under a weak alkaline condition through continuous heating and stirring, and a protocatechuic acid-zinc compound obtained through the reaction has an excellent broad-spectrum antibacterial effect.

Compositions and methods for converting at least one polyphenol to protocatechuic acid (PCA) using Bacillus subtilis 1579, or active variants thereof, are provided. Conversion of polyphenols, such as quercetin, to PCA can decrease inflammation, decrease cortisol levels, and increase milk quality and milk production quantity in milk-producing agricultural animals. Accordingly, provided herein are compositions comprising B. subtilis 1579, or an active variant thereof, for administration to humans for decreasing inflammation or for administration to milk-producing agricultural animals for decreasing inflammation, decreasing cortisol levels, and increasing milk quality and milk production.

This invention discloses the application of reagents for detecting biomarker levels in the preparation of products for identifying obstructive sleep apnea. It relates to the field of biomarker technology and aims to overcome the shortcomings of existing technologies that rely on complex polysomnography (PSG) procedures, poor patient compliance, and a lack of effective molecular markers. The biomarkers used in this application consist of CD36 glycoprotein, 9-octadecenal, and protocatechuic acid 3-O-sulfate. This invention enables rapid and objective identification of obstructive sleep apnea by detecting the levels of the above biomarkers in plasma. The procedure is simple and well-accepted by patients. The combined use of multiple biomarkers can improve the accuracy and stability of the identification, making it suitable for screening or auxiliary diagnosis in medical institutions at different levels.

The invention relates to the technical field of medicines, and discloses a bifidobacterium longum strain GZBAI 01 and application thereof in degradation of benzoic acid and prevention or treatment of colorectal cancer. The strain is separated from faeces of healthy people, genomics identification is carried out, the metabolic profile of the strain is measured through metabonomics, the strain can convert benzoic acid with carcinogenic risk into protocatechuic acid with antitumor activity through unique metabolic capability, meanwhile, the function of regulatory T cells (Treg) in tumor drainage lymph nodes can be inhibited, differentiation of CD8 + T cells is promoted, and the tumor drainage lymph nodes can be inhibited. Therefore, the colorectal cancer is prevented through double ways of eliminating cancerogen and generating therapeutic agents. A colorectal cancer mouse model and a subcutaneous tumor mouse model verify that the strain can significantly reduce the number and volume of tumors, and shows a benzoic acid conversion effect superior to that of other strains in vivo and in vitro, and a new way is provided for prevention and treatment of colorectal cancer.

The invention relates to the technical field of coatings, in particular to bionic raw lacquer prepared from protocatechuic acid and a preparation method of the bionic raw lacquer. According to the specific technical scheme, the method for preparing the bionic raw lacquer from protocatechuic acid comprises the following steps: (1) uniformly mixing protocatechuic acid, an alkylation modifier and a catalyst in a nitrogen atmosphere, carrying out a heating reaction, and after the reaction is completed, washing and drying an organic phase to obtain alkylation modified protocatechuic acid; (2) adding an emulsifier into the aqueous solution of the alkylated modified protocatechuic acid, and uniformly stirring to obtain an alkylated protocatechuic acid emulsion; adding a catalyst into the alkylated protocatechuic acid emulsion, dropwise adding a hydrogen peroxide solution, and reacting to obtain an alkylated protocatechuic acid emulsion polymer; and (3) uniformly mixing the alkylated protocatechuic acid emulsion polymer with a drier or a photoinitiator to obtain the product. The problems that in the prior art, natural raw lacquer resources are limited, and the curing rate of micromolecule bionic raw lacquer monomers is low are solved.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The invention relates to a construction method and a detection method of characteristic chromatograms of a Java amomum cardamomum medicinal material, a standard decoction and a traditional Chinese medicine formula granule. According to the method, proper ultra-high performance liquid chromatography conditions are selected, protocatechuic acid, (4R, 6R)-6-hydroxypiperidone, (4R, 5R)-4-Hydroxy-5-isopropyl-2-methylaminohex-2-enone, vanillic acid and (4S, 5R)-4-Hydroxy-5-isopropyl-2-methylaminohex-2-enone are used for preparing a reference substance solution, a Java amomum cardamomum medicinal material, a standard decoction or / and formula granules are used as a test sample for detection, a characteristic spectrum is constructed, and the characteristic spectrum is applied to the detection of the Java amomum cardamomum medicinal material, the standard decoction or / and the formula granules. The characteristic chromatogram contains 9 characteristic peaks, is rich in chromatogram information, can reflect the quality of the Java amomum cardamomum medicinal material, can reflect the quality of standard Java amomum cardamomum decoction and traditional Chinese medicine formula granules, can be used for detecting the quality of the Java amomum cardamomum medicinal material, the standard Java amomum cardamomum decoction and the traditional Chinese medicine formula granules, and has a wide application prospect. The invention provides an effective method for quality detection of the Java amomum cardamomum and the modern preparation thereof, and also provides a scientific experimental basis for research on comprehensive quality evaluation of the Java amomum cardamomum and the modern preparation thereof.

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

The invention discloses a method for synthesizing gallic acid through double-cell catalysis of terephthalic acid and a strain of the gallic acid through double-cell catalysis of terephthalic acid. The method comprises the following steps: respectively constructing a protocatechuic acid transformation strain for expressing tphAa, tphAb, tphAc and tphB genes in a tphAB gene cluster and a gene tpaK for coding TPA transport protein, and a gallic acid transformation strain for expressing a gene pobA for coding p-hydroxybenzoic acid hydroxylase; bacterial cells of the protocatechuic acid transformation strain and the gallic acid transformation strain are used as catalysts to catalyze a substrate terephthalic acid to synthesize gallic acid. The protocatechuic acid transformed strain constructed by the invention assists terephthalic acid to enter cells by using transporter, so that the intracellular concentration of terephthalic acid is remarkably improved, and the conversion rate of gallic acid produced and synthesized by terephthalic acid is further improved.

Aspects of the present disclosure relate to the use of engineered host cells capable of producing protocatechuic acid (PCA). Aspects of the present disclosure also relate to compositions comprising engineered host cells capable of producing protocatechuic acid and / or cell constituents thereof, and the use of such compositions for altering and / or maintaining biological activity in a subject.

A strain of Acanthopanax senticosus endophytic fungus HASZ2 producing isofraquinone and protocatechuic acid and its application, relating to the field of microorganisms, the Acanthopanax senticosus endophytic fungus HASZ2 is Rhodotorula glutinosus HASZ2, which has been preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, the preservation date is May 16, 2024, and the preservation number is CCTCC NO: M2024973. The strain has good antioxidant activity. After secondary metabolite analysis of its fermentation broth, it was found that the strain can produce isofraquinone and protocatechuic acid. The present invention is used for anti-oxidation and production of Acanthopanax senticosus secondary metabolites.

The present invention relates to processes for the formation of pyridinedicarboxylic acid (PDCA), in particular, 2,4-pyridinedicarboxylic acid (2,4-PDCA) and 2,5-pyridinedicarboxylic acid (2,5-PDCA), and mono- and diester derivatives thereof, from 3,4-dihydroxybenzoic acid, via a biocatalytic reaction using, for example, a protocatechuate dioxygenase such as protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase, and a nitrogen source. The invention also relates to copolymers that comprise the pyridinedicarboxylic acid monomers and derivatives thereof, processes for the formation of the copolymers and uses for the copolymers.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The invention relates to a traditional Chinese medicine clerodendranthus spicatus extract for treating nephritis. The extract contains tanshinol (content gt; 0.07%), protocatechuic acid (content gt; 0.12%), rosmarinic acid (content gt; 9%), alkannic acid (content gt; 1.2%), helelisters C (the content of gt; 4.0%), salvianolic acid B (content gt; 2.0%), salvianolic acid A (content gt; 2.0%). The invention further provides a pharmaceutical composition containing the extract provided by the invention, and the pharmaceutical composition is selected from any dosage form capable of being taken. The dosage form is selected from tablets, capsules, oral liquid, granules, pills, ointment, dripping pills and injections.