306 results about "Protocatechuic acid" patented technology

The invention relates to the field of biology, and discloses a serum-free culture medium which essentially comprises an IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, Hepes, recombinant human insulin, recombinant human transferrin, recombinant human albumin, 2-mercaptoethanol, protocatechuic acid, lipid, amino acid, vitamins, trace elements, Pluronic F-68, hydrocortisone, vitamin C, bonding amine or recombinant human fibronectin, progesterone, putrescine, heparin, serotonin, epidermal growth factors (EGFs), b-fibroblast growth factors (FGF), platelet derive growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I. The serum-free culture medium is clear in chemical components, free from animal sources and serum and safe and ideal in cell cultivation and avoids the doped animal components and unstable batches, and the results of the cultured mesenchymal stem cells show that the total cellular score, the cell phenotype and the secretory cell factors are normal, so that the serum-free culture medium has good industrial application prospect.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention discloses total phenyl propanoid extract and total phenol extract extracted from grassleaved sweetflag rhizome and its preparation method. Total phenyl propanoid extract mainly comprises beta -asaricin, alpha -asaricin, eudesmin, galgravin, veraguensin and other derivant with veraguensin as mother nuclide. Total phenol extract mainly comprises caffeic acid, protocatechuic acid, ferulic acid, vanillic acid and glucosides with vanillic acid as mother nuclide and other derivants. Total phenyl propanoid extract and total phenol extract of grassleaved sweetflag rhizome can be extracted through one or more of methods of solvent-extraction, solvent extraction, precipitation method, macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Total percentage content of phenyl propanoids in the obtained total phenyl propanoid extract of Grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of beta -asaricin and alpha -asaricin occupy 5-100% (w/w).Total percentage content of phenols in total phenol extract of grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of caffeic acid and protocatechuic acid occupy 5-100% (w/w).

The invention provides a method for extracting compound from kidney tea stalks and leaves, crushing the stalks and leaves, successively adopting water extraction, alcohol extraction, and ethyl acetate to extract water soluble, alcohol soluble and fat soluble compounds, which are rosemary acid, caffeic acid, bear fruit acid, Yuan er tea acid, isoflavone, rosemary acid acetate and other phenolic compounds and flavone compounds. The invention is a kidney tea compound extracting method, effective and applied to industrial production.

The invention provides an application of protocatechuic acid in the preparation of drugs for preventing and controlling livestock and poultry virus infectious diseases. The viruses include infectious bursal disease virus, avian influenza virus, infectious bronchitis virus and/or porcine transmissible gastroenteri tis virus. Meanwhile, the invention also relates to a protocatechuic acid preparation and a preparation method thereof.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The invention discloses a preparing method of protocatechuic acid, which comprises the following steps: adsorbing extracted salvia miltiorrhizae alkaline through weak base resin; adjusting pH value of eluent at 1-4; eluting through pure water or acid first and low-density alcohol then; collects alcohol cleaning solution; condensing; cooling; fitting for large scale of industrial manufacturing.

The invention discloses a green tea extract-ferroferric oxide composite catalyst and application thereof. A preparation method of the green tea extract-ferroferric oxide composite catalyst comprises the steps of dissolving FeCl3.6H2O, sodium acetate and polyethylene glycol into ethylene glycol, then placing the mixture into a reaction kettle, raising the temperature to 195-205 DEG C, preserving the temperature for 8-72 hours, and then conducting natural cooling to reach the room temperature, so that black solid is obtained; cleaning the black solid with ethanol and ultra-pure water respectively, and conducting vacuum drying, so that ferroferric oxide powder is obtained; weighing ferroferric oxide and green tea extract according to the mass ratio of 1: 5-5: 1, placing the ferroferric oxide and the green tea extract in the ultra-pure water, conducting ultrasonic treatment for 0.5-2 hours, and conducting solid-liquid separation; conducting vacuum drying on obtained solid, so that the green tea extract-ferroferric oxide composite catalyst is obtained, wherein the green tea extract is one or more of protocatechuic acid, epigallocatechin, gallate and epicatechin. The green tea extract has great reductibility, and ferroferric oxide catalysis efficiency can be improved; the composite catalyst is green and environmentally friendly and convenient to separate, and secondary pollution cannot be caused.

The invention discloses a quality control method for simultaneous realization of content analysis and similarity evaluation of 18 components in Ilex kudingcha. Rutin, isochlorogenic acid A and kudinoside A are used as internal references; correction factors of rutin for 6-hydroxy-7,7a-dihydro-2(6H)-benzofuran and hydroxytyrosol glucoside, correction factors of isochlorogenic acid A for protocatechuic acid, kudinoside E, kudinoside D, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, and isochlorogenic acid C, and correction factors of kudinoside A for latifoloside G, kudinoside G, ilex kudingcha ilexoside T and latifoloside H are calculated, and the factors are used as constants for determining content. Only three common reference substances are needed for simultaneous determination of contents of 18 kinds of components in ilex kudingcha, quality of the ilex kudingcha can be rapidly, economically and scientifically controlled, and further cluster analysis, main component analysis and similarity calculation of medicinal materials can be carried out by using contents of the 18 kinds of components, in order to comprehensively control quality of ilex kudingcha.

The invention belongs to the technical field of quality control of Chinese materia medica preparations, and provides a method for measuring contents of multiple components in Shenxiong glucose injection. According to the invention, the HPLC (high performance liquid chromatography) is adopted to measure contents of six compounds, namely tanshinol, ligustrazine hydrochloride, protocatechuic acid, rosmarinic acid, danshinolic acid B and danshinolic acid A, in the Shenxiong glucose injection under the same chromatographic condition. Compared with the present simple content measurement method for a single component, the method provided by the invention is better in integrity, characteristics and stability, can comprehensively reflect the interior quality of the preparations, and provides test base for further improving the quality control level of the Shenxiong glucose injection and ensuring the quality stability of the products.

Belonging to the technical field of traditional Chinese medicine extraction, the invention discloses a technological method for extraction of protocatechuic acid from Blumea riparia (Bl.) DC. The method for preparation of a protocatechuic acid monomer consists of: water extraction, concentration, extraction, elution, and TLC preparation and purification. A compound B is determined as protocatechuic acid with a concentration of equal to or more than 99% by HPLC analysis, and is the protocatechuic acid monomer. The technological method for extraction of chlorogenic acid from Blumea riparia (Bl.) DC solves the problems of low preparation purity, small preparation quantity, complex preparation process, and difficult realization of industrialization production. By means of water extraction, concentration, extraction, elution and other processes, high extraction purity and a simple preparation process can be realized. Thus, the method is suitable for popularization, and meets the need of people for protocatechuic acid.

The invention discloses a novel method for simultaneously measuring contents of multiple active ingredients of dogwood. The method is used for simultaneously measuring the contents of seven ingredients, namely loganin, gallic acid, 5-HMF (hydroxymethyl furfural), protocatechuic acid, morroniside, sweroside and cor-nuside, in dogwood medicinal materials from four main producing areas by utilizing a high performance liquid diode array detection method. The method comprises the steps of: carrying out gradient elution at the flow speed of 1.0 mL.min-1 by utilizing a Phenoemenex C18 polar chromatographic column and taking acetonitrile and 0.1% methanoic acid solution as mobile phases, wherein the column temperature is 30 DEG C, the detection wavelengths are 220 nm, 240 nm, 260 nm, 265 nm and 280 nm. The contents of the loganin, the gallic acid, the 5-HMF, the protocatechuic acid, the morroniside, the sweroside and the cor-nuside in each sample are respectively 0.22-0.800 mg/g, 0.002-0.024 mg/g, 0.069-3.775 mg/g, 8.192-28.111 mg/g, 0.048-0.598 mg/g, 1.228-9.311 mg/g and 1.450-8.381 mg/g. The method is simple and accurate, is good in reproducibility and is capable of providing bases for comprehensively evaluating and controlling the quality of dogwood decoction pieces.

The invention discloses a method for establishing an HPLC fingerprint spectrum of a Zhuang medicinal material Blumea riparia (Bl.) DC. By adopting a high-performance liquid chromatography, and using main ingredients such as protocatechuic acid and protocatechuic aldehyde of Blumea riparia (Bl.) DC as reference substances, a fingerprint spectrum of a common pattern of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material is obtained. The fingerprint spectrum has 13 chromatographic peaks, can fully reflect the chemical compositions of the Blumea riparia (Bl.) DC medicinal material, and is rich in information amount, the method has good reproducibility, a more powerful theoretical basis is provided for controlling the quality of the medicinal material and identifying the advantages and disadvantages of the medicinal material, the quality control and the level of true and false identification of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material are improved, and the authenticity and the advantages and disadvantages of the Blumea riparia (Bl.) DC can be quickly and accurately identified.

The invention belongs to the field of microbial herbicides and particularly relates to weed suppression fungus screened from passion fruit rhizosphere soil. Lettuce (compositae), barnyard grass (gramineae) and lysimachia barystachys (gramineae) are taken as receptors separately, biological test is carried out on the weed suppression ability of a fungus strain fermentation liquid, and the condition that the weed suppression fungus has a very high inhibition rate on root length and plant height of two receptors is found out. The condition that protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, salicylic acid and cinnamic acid contain phenolic acid with a weed suppression effect is detected in the fermentation liquid. The strain is named FJ-01-BXG-08. The analysis and identification results on the morphology and ITS sequence of the strain show that the strain is one of Aspergillus sydowii (aspergillus sydowii), and strong weed suppression fungus aspergillus sydowii (Aspergillus sydowii) is screened from eucalyptus soil for the first time.

The invention relates to a method for promoting an Fe(III)/H2O2 system to repair organism polluted water by utilizing protocatechuic acid. The method comprises the following steps: adding the protocatechuic acid and a Fe3+ substance into the organism polluted water to be repaired; and controlling the system to enable the system is under the acid condition, adding H2O2 to carry out repair of the organism polluted water. The method solves the problems of easiness for precipitation of Fe3+ and difficulty in circulation of Fe3+/Fe2+ in conventional Fenton and Fenton-like systems and has the advantages of high efficiency, non preference, environmental-friendliness, no secondary pollution and the like when being used for repairing the organism polluted water.

The invention discloses a structural general formula of a compound LQC-T and the synthesis and the application of the compound LQC-T. Pharmacological experiments prove that the compound has an obvious effect of promoting new vessels of chick chorioallantoic membranes to grow; a compound LQC-T4 has an obvious medicinal effect of treating ischemic brain injury stroke and the sequela of the ischemic brain injury stroke; the maximal LQC-T4 single-day dose of mice is 5,400mg/kg; and the compound is extremely high in safety and can be used for preparing a medicine for treating the ischemic brain injury stroke and the sequela of the ischemic brain injury stroke after toxic responses do not occur after continuous observation within 1 to 4 days. According to the structural formula of the compound LQC-T, R is protocatechuic acid, protocatechuic aldehyde, vanillic acid, gallic acid, caffeic acid, ferulic acid and other aromatic organic acids or phenols or structural analogues of the organic acids or phenols.

The invention relates to a method for measuring drug contents, particularly to a method for measuring danshensu, m-methyl-danshensu, protocatechualdehyde, and protocatechuic acid in the human blood plasma. The method comprises the following steps: 1) preparation of reference substance storing solutions: weighting reference substances of danshensu, m-methyl-danshensu, protocatechualdehyde, and protocatechuic acid, and dissolving the reference substances with methanol to obtain reference substance storing solutions of danshensu, m-methyl-danshensu, protocatechualdehyde, and protocatechuic acid; 2) preparation of an internal standard solution: dissolving an internal standard substance of vanillic acid with methanol to obtain the internal standard solution; 3) a treatment method of a blood plasma sample: taking the blood plasma sample, adding the internal standard solution, methanol, hydrochloric acid, and ethyl acetate into the blood plasma sample, performing uniform mixing, performing centrifugation of the obtained mixture to obtain a supernatant ethyl acetate layer, drying the ethyl acetate layer, re-dissolving the ethyl acetate layer with methanol, and performing centrifugation of the obtained solution to obtain supernatant liquid of the blood plasma sample; and 4) content measurement: by adopting LC-MS/MS chromatography, injecting the reference substance storing solutions and the supernatant liquid of the blood plasma sample into a chromatographic instrument to obtain a chromatogram, and calculating concentrations of danshensu, m-methyl-danshensu, protocatechualdehyde, and protocatechuic acid in the human blood plasma according to the areas of chromatographic peaks in the chromatogram.

The invention discloses a preparation method of a phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus. According to the preparation method, mainly chitosan and protocatechuic acid molecules are adopted as reaction substrates, and a chitosan-protocatechuic acid grafted copolymer is synthesized by using an EDC-mediated cross-linking reaction. According to the present invention, the synthesis process is simple, and the obtained chitosan-protocatechic acid grafted copolymer has characteristics of good water solubility, high grafting rate and high antioxidant activity, and can significantly improve the storage quality of edible fungus and prolong the shelf life; with the preparation method, the problems of poor water solubility, low oxidation activity and the like of the chitosan can be solved so as to improve the performance of the chitosan and expand the application in the storage and the fresh keeping of food; and the method is less investment, is suitable for large-scale production, and has excellent application prospect.

The invention discloses a rice wine brewing method for reducing the content of ethyl carbamate through protocatechuic acid. The method includes the steps of rice steeping, rice steaming, water spraying, cylinder falling for nest building, wheat cojic and water adding, fermentation and after-treatment; protocatechuic acid is added to fermentation liquor 2-5 days after cylinder falling for nest building. Protocatechuic acid is added at the fermentation stage, so that conversion of precursor substances citrulline and urea of ethyl carbamate into ethyl carbamate in the rice wine brewing process is effectively restrained, and then the content of ethyl carbamate in rice wine is reduced. Protocatechuic acid belongs to inherent phenolic substances in the rice wine, multiple biological activities are achieved, no follow-up treatment is needed during addition of protocatechuic acid, and the defects of an existing way for degrading ethyl carbamate can be effectively overcome.

A Chinese medicine 'Fuxuekang' in the form of tablet or capsule is prepared from balsamiferous blumea and protocatechuic acid through extracting of balsamiferous blumea, drying, and proportionally mixing it with protocatechuic acid.