102 results about "Protocatechuic acid" patented technology

The invention discloses an absorbable suture line containing fibroin and a preparation method of the absorbable suture line, and relates to the technical field of absorbable suture lines. The absorbable suture line containing the silk protein is prepared from the following raw materials in parts by weight: 35 to 50 parts of silk protein, 10 to 20 parts of polylactic acid, 15 to 25 parts of polyglycolide-lactide, 1 to 3 parts of antibacterial agent, 2 to 5 parts of modified toughening agent, 1 to 4 parts of nano mineral filler, 0.5 to 2 parts of cross-linking agent and 2 to 5 parts of plasticizer, the preparation method comprises the following steps: reacting protocatechuic acid with ethanolamine to generate an intermediate 1; and reacting the intermediate 1 with 5, 10, 15, 20-tetra (4-carboxyl phenyl) porphyrin to generate the antibacterial agent. The prepared absorbable suture line containing the silk protein has good mechanical performance, self-repairing performance and antibacterial performance.

The invention provides an aluminum alloy pretreatment method based on complex reaction, which comprises the following steps: S1, polishing an LDH (Layered Double Hydroxides) growth surface by using abrasive paper, and cleaning and drying; s2, preparing a complexing agent solution, wherein a complexing agent is selected from at least one of tannic acid, gallic acid and protocatechuic acid; s3, the aluminum alloy sample treated in the step S1 is put into the complexing agent solution in the step S2, and constant potential is applied to the aluminum alloy; s4, preparing a mixed solution of Zn (NO3) 2.6 H2O and NH4NO3, and adding an ammonia water solution to adjust the pH value to 6-6.5; s5, immersing the aluminum alloy treated in the step S3 into the mixed solution in the step S4, and putting the aluminum alloy into a constant-temperature water bath kettle to generate a Zn-Al-LDH film; and S6, after the hydrothermal reaction is finished, cooling to room temperature, and washing and drying the obtained reaction product. The surface of the aluminum alloy is pretreated through the complexing agent, the potential between an intermetallic compound and an aluminum matrix can be effectively balanced, the micro-couple effect is fundamentally inhibited, and ideal conditions are created for uniform, compact and defect-free growth of LDH.

This invention discloses the application of reagents for detecting biomarker levels in the preparation of products for identifying obstructive sleep apnea. It relates to the field of biomarker technology and aims to overcome the shortcomings of existing technologies that rely on complex polysomnography (PSG) procedures, poor patient compliance, and a lack of effective molecular markers. The biomarkers used in this application consist of CD36 glycoprotein, 9-octadecenal, and protocatechuic acid 3-O-sulfate. This invention enables rapid and objective identification of obstructive sleep apnea by detecting the levels of the above biomarkers in plasma. The procedure is simple and well-accepted by patients. The combined use of multiple biomarkers can improve the accuracy and stability of the identification, making it suitable for screening or auxiliary diagnosis in medical institutions at different levels.

The invention relates to the technical field of medicines, and discloses a bifidobacterium longum strain GZBAI 01 and application thereof in degradation of benzoic acid and prevention or treatment of colorectal cancer. The strain is separated from faeces of healthy people, genomics identification is carried out, the metabolic profile of the strain is measured through metabonomics, the strain can convert benzoic acid with carcinogenic risk into protocatechuic acid with antitumor activity through unique metabolic capability, meanwhile, the function of regulatory T cells (Treg) in tumor drainage lymph nodes can be inhibited, differentiation of CD8 + T cells is promoted, and the tumor drainage lymph nodes can be inhibited. Therefore, the colorectal cancer is prevented through double ways of eliminating cancerogen and generating therapeutic agents. A colorectal cancer mouse model and a subcutaneous tumor mouse model verify that the strain can significantly reduce the number and volume of tumors, and shows a benzoic acid conversion effect superior to that of other strains in vivo and in vitro, and a new way is provided for prevention and treatment of colorectal cancer.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

Aspects of the present disclosure relate to the use of engineered host cells capable of producing protocatechuic acid (PCA). Aspects of the present disclosure also relate to compositions comprising engineered host cells capable of producing protocatechuic acid and / or cell constituents thereof, and the use of such compositions for altering and / or maintaining biological activity in a subject.

The present invention relates to processes for the formation of pyridinedicarboxylic acid (PDCA), in particular, 2,4-pyridinedicarboxylic acid (2,4-PDCA) and 2,5-pyridinedicarboxylic acid (2,5-PDCA), and mono- and diester derivatives thereof, from 3,4-dihydroxybenzoic acid, via a biocatalytic reaction using, for example, a protocatechuate dioxygenase such as protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase, and a nitrogen source. The invention also relates to copolymers that comprise the pyridinedicarboxylic acid monomers and derivatives thereof, processes for the formation of the copolymers and uses for the copolymers.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

This invention discloses a mitochondrial-targeted nanomaterial for senescent cells and its application in anti-aging. The material is a nucleic acid aptamer-tetrahedral DNA, mainly assembled from four single-stranded DNA sequences, SEQ ID Nos. 1-4. Preparation involves first hybridizing the four single-stranded DNA sequences, then concentrating them to obtain aptamer-modified tetrahedra, which are then coupled with tannic acid (TA) via a Mann reaction in the presence of formaldehyde to obtain the Apt-tFNAs-TA complex. Finally, it is incubated with excess catechins to obtain the final delivery material, Apt-tFNAs@catechin-TA. This invention's material exhibits good biocompatibility and skin tissue compatibility, significantly improving uptake efficiency and mitochondrial localization ability in senescent cells. It can effectively deliver polyphenolic compounds such as catechins and protocatechuic acid, improving mitochondrial function and addressing the technical problems of low delivery efficiency of active ingredients and insufficient targeted enrichment in senescent cells.

The application discloses an aluminum profile spraying powder coating and a preparation method thereof, and belongs to the technical field of paint. The application introduces modified resin, so that the coating has excellent weather resistance, and adds specific proportion of proto-catechuic acid, 2-(4-thiazolyl) benzimidazole and 8-hydroxyquinoline as an antibacterial agent, and chemically bonds the antibacterial agent to the resin base material, so that efficient, long-lasting and broad-spectrum antibacterial effect is realized, and more significant antibacterial purpose is realized, and the problems of easy migration and easy failure of the surface spraying antibacterial agent are effectively solved. The application optimizes the overall formula, and the components interact with each other, so that the coating has excellent surface flatness and apparent quality, and the defects of pinholes and orange peel commonly existing in traditional coatings are avoided; the overall antibacterial and long-lasting antibacterial properties are significant, and the application is particularly suitable for buildings, medical treatment and household aluminum profiles with high sanitary requirements.

The application discloses a construction method of a UPLC characteristic spectrum of a radix-lithospermi and application thereof, 1) preparation of a control medicinal material reference solution; 2) preparation of a control reference solution: a certain amount of protocatechuic acid, protocatechuic aldehyde, ferulic acid, rosmarinic acid, caffeic acid tetramer and danshensu are taken to prepare a mixed solution by adding methanol, serving as the control reference solution; 3) preparation of a test solution; 4) determination: the control medicinal material reference solution, the control reference solution and the test solution are respectively taken and injected into a liquid chromatograph to determine characteristic spectra, wherein the characteristic spectra include at least 12 characteristic peaks; 5) qualitative analysis of a sample to be determined is carried out by using the characteristic spectra obtained in the above steps. The characteristic spectrum includes 12 characteristic peaks, can effectively distinguish radix-lithospermi (Xinjiang radix-lithospermi) and its pseudo products of different origins, and embodies the integrity of quality control of radix-lithospermi.

The present invention belongs to the technical field of hydrogel preparations, and specifically relates to a dual-responsive hydrogel, a preparation method, and an application thereof. The present invention synthesizes a cross-linked polycationic hydrogel composed of phenylboronic acid-modified sodium alginate, a protocatechuic acid-polyethyleneimine graft polymer, and bevacizumab sustained-release nanoparticles, referred to as a dual-responsive OSPPB hydrogel. This hydrogel blocks neurovascularization at the osteochondral interface by locally clearing extracellular RNA and releasing bevacizumab, thereby alleviating OA pain and disease progression.

The present invention relates to the field of adhesive materials, and in particular to a protocatechuic acid-modified epoxy resin, and its preparation method and application. The preparation method of the modified epoxy resin comprises: using protocatechuic acid and epoxy resin I as raw materials to carry out a chain extension reaction; wherein the epoxy resin I is one or a combination of several of bisphenol A epoxy resin, bisphenol F epoxy resin, ethylene glycol diglycidyl ether, and polypropylene glycol diglycidyl ether. The adhesive made from the modified epoxy resin has good room temperature bonding strength and high temperature bonding strength; specifically, the shear strength of the adhesive at room temperature reaches more than 20MPa, the shear strength at 150°C reaches more than 10MPa, the peel strength at room temperature reaches 3KN / m, and the aging performance at 250°C is also significantly improved.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The invention provides a mesenchymal stem cell serum-free culture solution and application thereof, and belongs to the technical field of cell culture. The mesenchymal stem cell serum-free culture solution provided by the invention is prepared from UltraGRO-Advanced, adrenocorticotropin, testosterone, curcumin, protocatechuic acid, TNF-alpha (tumor necrosis factor-alpha), GM-CSF (granulocyte-macrophage colony-stimulating factor), SCF (mesenchymal stem cell), insulin, a lipid mixture and the balance of a basic culture medium, the culture solution can remarkably improve the proliferation efficiency of cells and improve the osteogenic differentiation capacity.

The application provides an extraction method of a vine tea extract and the extract. Primarily extracted is protocatechuic acid, and the extraction method mainly comprises a processing step, wherein the vine tea is steamed at 110-120 DEG C for 0.5-1.5 h, and then cut into segments for standby. The application also provides an application of the extract, which is used for preparing antioxidant and anti-inflammatory drugs and / or food and / or cosmetics. The application has the advantages that the extraction efficiency of protocatechuic acid is effectively enhanced through the synergistic effect of processing and extraction processes, the extraction rate is greatly increased from 0.1-0.6 mg / g in the prior art to 1-1.4 mg / g, and the increase is significant.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

The invention discloses a Lactobacillus paracasei strain capable of converting truffle to generate various active small molecules, regulating and controlling collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. The lactobacillus paracasei CCFM1493 obtained through screening can produce m-hydroxyphenylacetic acid and salicylic acid by fermenting white truffle, the content of protocatechuic acid and dihydrocaffeic acid is increased, the skin quality is improved by regulating skin cell collagen synthesis, oxidation resistance and aging resistance, and it can be seen that the lactobacillus paracasei CCFM1493 can be used for preparing the traditional Chinese medicine composition. The fermentation liquor of the white truffle fermented by the Lactobacillus paracasei (Lactobacillus paracasei) CCFM1493 has a huge application prospect in the preparation of a product for improving the skin quality.

The present invention discloses a method for constructing a characteristic spectrum of a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction, comprising the following steps: preparing a reference solution, the reference solution comprising a control medicinal material reference solution and a reference substance reference solution, the reference substance reference solution comprising a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance, and a chlorogenic acid reference substance; taking a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction to prepare a test solution; taking a preset amount of the reference solution and the test solution, injecting them into a liquid chromatograph, the liquid chromatograph using octadecylsilane bonded silica gel as a filler, methanol as mobile phase A, and phosphoric acid solution as mobile phase B for gradient elution to establish a characteristic spectrum. The present invention can provide a data basis for the quality control of Corydalis guanzhong medicinal materials, formula granules, decoction pieces or standard decoctions, and ensure the stability and controllability of product quality.

This invention belongs to the field of drug detection, specifically relating to a method for constructing a fingerprint spectrum of Cibotium barometz and a method for determining the content of its active ingredients. The construction method includes the preparation of a test solution and detection using high-performance liquid chromatography (HPLC) to obtain the fingerprint spectrum of Cibotium barometz. This invention establishes a comprehensive and rapid method for detecting Cibotium barometz drug preparations, which is of great significance for its comprehensive quality detection and overall quality control. The characteristic spectrum obtained by this construction method has at least 12 common characteristic peaks, a stable baseline, and good peak resolution. Furthermore, the relative retention times of each characteristic peak are calculated based on protocatechuic acid, which is beneficial for the comprehensive quality detection and overall quality control of Cibotium barometz preparations, and helps improve the safety and stability of drug use.

The invention discloses a low caffeine nerve stimulation tea wine based on litchi wood and sandalwood and a preparation process thereof, the wine is mainly prepared by blending litchi wood barrel aged tea wine and litchi wood / sandalwood barrel aged tea wine with different baking degrees, and the mass ratio of protocatechuic acid to catechin to epicatechin in the wine body is (0.15-0.28): 1: (0.15-0.25). The mass ratio of the protocatechuic acid to the alpha-santalol to the beta-santalol is 1: (0.4-0.6), the mass ratio of the quercetin to the gallic acid to the ferulic acid is (1.7-2.5): 1: (2.5-3.5), the mass ratio of the protocatechuic acid to the alpha-santalol is (1-2.5): 1, and the total content of the protocatechuic acid, the catechin, the epicatechin, the alpha-santalol, the beta-santalol, the quercetin, the gallic acid and the ferulic acid in the wine is 60-600mg / 100mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The invention relates to the technical field of liqueur, in particular to liqueur and a preparation method thereof.The liqueur is prepared by soaking various medicinal and edible raw materials such as kudzuvine roots, rice, barbary wolfberry fruits, smoked plums, grapes, mulberries, poria cocos, Chinese yams, rhizoma polygonati, jujubes and ginseng in baijiu base liquor according to a specific proportion. According to the present invention, the modern analysis technology shows that the wine contains 25 active components such as resveratrol, protocatechuic acid, oleanolic acid, quercetin, kaempferol and the like, and the components achieve the synergistic effect, such that the product has effects of tumor resistance, liver protection, blood sugar reduction, blood fat reduction, oxidation resistance, inflammation resistance, nerve protection and other health-care functions, and the product has characteristics of definite component, synergistic effect, high safety, and no toxic-side effect. The preparation process is stable and controllable, and is suitable for large-scale production.

The present invention provides a hair coating composition comprising: a first polyphenol including at least two among tannic acid, gallic acid, and caffeic acid; and a second polyphenol comprising at least one selected from the group consisting of a biphenyl moiety, dopamine, catechin, anthocyanin, resveratrol, quercetin, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, ferulic acid, luteolinidin, apigeninidin, and thureolin, wherein the composition can restore and improve the mechanical strength of hair through a Michael addition reaction with a thiol group included in damaged hair.

The invention discloses protocatechuic acid O-methyltransferase based on artificial intelligence design and a design method of the protocatechuic acid O-methyltransferase, the amino acid sequence of the protocatechuic acid O-methyltransferase is SEQ ID NO: 1 or SEQ ID NO: 2, the active center of the protocatechuic acid O-methyltransferase comprises an octahedral coordination configuration formed by coordination of Mg < 2 + > and six coordination atoms, the protocatechuic acid O-methyltransferase is completed through artificial intelligence design; the similarity between the amino acid sequence of the obtained protocatechuic acid O-methyltransferase and the amino acid sequence BLAST of natural protocatechuic acid O-methyltransferase is relatively low, and the designed protocatechuic acid O-methyltransferase can be used for remarkably improving the enzymatic activity and the purity of a target product.

The invention provides a genetic engineering recombinant bacterium for producing catechol through whole-cell catalysis and a production method of the genetic engineering recombinant bacterium. Genetic engineering recombinant bacteria for expressing decarboxylase serve as a whole-cell catalyst, protocatechuic acid serves as a substrate, efficient whole-cell catalytic conversion from protocatechuic acid to catechol is achieved, and no cofactor needs to be added in the whole catalytic process. The method for producing catechol through whole-cell catalysis, provided by the invention, has the characteristics of high synthesis efficiency, no need of adding cofactors and simplicity in operation, and has important industrial application value.

The invention provides a medicament for treating a milk disease of eriocheir sinensis and a preparation method of the medicament. The medicament is prepared from the following raw materials in parts by weight: 6-7 parts of traditional Chinese medicine components, 2 parts of allicin and 1 part of an additive, wherein the traditional Chinese medicine components comprise coptis chinensis, golden cypress, schisandra chinensis, astragalus membranaceus and gallnut, and the additives comprise epsilon-polylysine, protocatechuic acid and rosmarinol. According to the medicament for treating the milk disease of the eriocheir sinensis, the efficacy limitation of a single natural medicine is broken through through a technology of combining the compound traditional Chinese medicine components with the allicin and the additive, the application of the natural medicine of aquatic products is promoted, a culture mode is assisted to be transformed to be green and efficient, and a brand new thought is provided for treating the milk disease.

The invention belongs to the technical field of medicines, and particularly relates to application of hibiscus (the hibiscus is a plant hibiscus flower and an extract and an active monomer thereof) in preparation of medicines for treating stomach diseases (atrophic gastritis, gastric mucosal lesion and gastric cancer). The invention discloses application of hibiscus syriacus in treatment of gastric diseases. The application comprises at least one of chronic atrophic gastritis treatment, gastric mucosa protection and gastric cancer prevention. In-vitro experiments show that the shrub althea flower extract, shrub althea flower polyphenol, syringic acid (I), protocatechuic acid (II), kaempferol (III) and kaempferol-7-O-beta-D-glucoside (IV) have obvious antioxidant activity and gastric mucosa protection activity, can obviously reduce the ROS content in ethanol-induced GES-1 cells and the expression of apoptosis marker protein Caspase-3 / Bax, and have a wide application prospect.

The invention belongs to the technical field of solar cells, and particularly discloses a high-stability monocrystalline silicon alkali polishing additive and a use method thereof. The invention relates to a high-stability monocrystalline silicon alkali polishing additive which comprises the following components in percentage by mass: 0.1%-2% of a polishing agent, 0.05%-0.4% of a corrosion and scale inhibitor, 0.1%-0.4% of a defoaming agent, 0.2%-1.6% of an auxiliary protective agent, 0.2%-0.5% of a surfactant and the balance of deionized water, the auxiliary protective agent is one or more of tannic acid, protocatechuic acid, tea polyphenol, caffeic acid and hydroxyphenethyl alcohol; the auxiliary protective agent is added into the additive, effective protection is provided for the alkali polishing additive, the stable polishing effect can be maintained at the high temperature for a long time, and then the photoelectric conversion efficiency of the battery can be improved.

The invention belongs to the technical field of plant disease prevention and control, and particularly relates to application of epicatechin and protocatechuic acid in prevention and control of citrus huanglongbing. Through systematic experimental research and application verification, it is found that epicatechin and protocatechuic acid can remarkably inhibit the number of citrus huanglongbing pathogenic bacteria, have a good synergistic effect, can remarkably improve the overall health condition of citrus trees and have excellent application prospects and popularization value. As epicatechin and protocatechuic acid have the characteristics of being natural, environment-friendly and efficient, the epicatechin and protocatechuic acid show important application potential in the field of prevention and treatment of the citrus huanglongbing, and are expected to become novel key tools in the field. The discovery not only provides a new thought for green prevention and control of the citrus huanglongbing, but also provides powerful technical support for sustainable development of the global citrus industry, and is of great significance for guaranteeing stable development of the industry.