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Method for establishing HPLC fingerprint spectrum of Zhuang medicinal material Blumea riparia (Bl.) DC

A fingerprint spectrum and a technology for establishing a method are applied in the field of establishing HPLC fingerprints of Zhuang medicinal material Diangui Ainaxiang, which can solve the problem that the overall quality of Zhuang medicine Diangui Ainaxiang cannot be well represented, and the chemical composition of samples is not fully reflected. The problem of the small number of identifiable components, etc., can improve the fingerprint identification ability, improve the quality control, and achieve the effect of scientific and method reproducibility.

Active Publication Date: 2015-03-25
广西万寿堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the pharmacognosy and chemistry of Zhuang medicine Ainaxiang Diangui are mainly characterized by character identification, microscopic identification and physical and chemical identification methods. The TLC identification method is also used in the drafting instructions of its quality standards, but these methods exist to provide The amount of information is small, the chemical composition of the sample is incomplete, and the number of identifiable components is small, so it cannot represent the overall quality of the Zhuang medicine Diangui Ainaxiang.

Method used

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  • Method for establishing HPLC fingerprint spectrum of Zhuang medicinal material Blumea riparia (Bl.) DC
  • Method for establishing HPLC fingerprint spectrum of Zhuang medicinal material Blumea riparia (Bl.) DC
  • Method for establishing HPLC fingerprint spectrum of Zhuang medicinal material Blumea riparia (Bl.) DC

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1 Experimental materials and instruments

[0028] 1.1 Medicinal materials: place of origin and harvest time are shown in Table 1.

[0029] Table 1 The origin and harvest time of the medicinal materials of Yunnan Gui Ainaxiang

[0030]

[0031]

[0032] 1.2 Instrument: Agilent1100 high performance liquid chromatography (including G1311A quaternary pump, G1313A autosampler, G1314A VWD detector, G1316A column thermostat, Agilengt1100 chromatographic workstation (Agilent Technologies, USA)) Electronic analytical balance: BP211D (Germany Doris); PS-60AL Ultrasonic Cleaner (40kHz, 360W); "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004 A Edition (National Pharmacopoeia Commission).

[0033] 1.3 Reagents: protocatechuic acid reference substance (purchased from China Institute for the Control of Pharmaceutical and Biological Products) batch number 110809-200604; protocatechualdehyde reference substance (purchased from China Institute for th...

Embodiment 2

[0043] Take 13 batches of Zhuang medicine Diangui Ainaxiang medicinal materials, detect according to the conditions of Example 1, and obtain the overlay of the HPLC fingerprints of 13 batches of samples, as shown in image 3 shown. By comparing these 13 batches of HPLC chromatograms, carry out acquaintance evaluation, determine its characteristic common peak: there are 13 characteristic common peaks in the fingerprint chromatogram, its retention time, the average value of peak area and the ratio of total peak area are summarized as follows:

[0044] Peak No. 1 has an average retention time of 5.919min, an RSD% of 0.23%, a peak area of ​​294.446, and an RSD% of 70.14%, accounting for 4.027% of the total peak area;

[0045] Peak No. 2 has an average retention time of 14.515min, an RSD% of 0.12%, a peak area of ​​960.173, and an RSD% of 26.12%, accounting for 13.131% of the total peak area;

[0046] Peak No. 3 has an average retention time of 17.867min, an RSD% of 0.08%, a peak ...

Embodiment 3

[0063] 1. Methodological investigation

[0064] 1.1 Precision experiment

[0065] Get numbered and be No. 6 need testing sample, prepare by need testing solution preparation method in embodiment 1, record fingerprint spectrum under above-mentioned chromatographic conditions. The results showed that the relative retention time RSD values ​​of each chromatogram were 0.068%-0.204%, and the relative peak area RSD values ​​of each peak were 0.045-1.76%. It shows that the precision of the instrument is good.

[0066] 1.2 Stability experiment

[0067] Get numbered and be No. 6 need testing sample, prepare by embodiment 1 need testing solution preparation method, under above-mentioned chromatographic condition, detect fingerprint at 0,4,8,12,16,24h respectively, investigate the repetition of experimental method sex. The results showed that the relative retention time RSDs of the 6 samples and chromatographic peaks were in the range of 0.038-0.336%, and the relative peak area RSD v...

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Abstract

The invention discloses a method for establishing an HPLC fingerprint spectrum of a Zhuang medicinal material Blumea riparia (Bl.) DC. By adopting a high-performance liquid chromatography, and using main ingredients such as protocatechuic acid and protocatechuic aldehyde of Blumea riparia (Bl.) DC as reference substances, a fingerprint spectrum of a common pattern of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material is obtained. The fingerprint spectrum has 13 chromatographic peaks, can fully reflect the chemical compositions of the Blumea riparia (Bl.) DC medicinal material, and is rich in information amount, the method has good reproducibility, a more powerful theoretical basis is provided for controlling the quality of the medicinal material and identifying the advantages and disadvantages of the medicinal material, the quality control and the level of true and false identification of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material are improved, and the authenticity and the advantages and disadvantages of the Blumea riparia (Bl.) DC can be quickly and accurately identified.

Description

technical field [0001] The invention belongs to the technical field of fingerprints of traditional Chinese medicinal materials in drug analysis, and in particular relates to a method for establishing HPLC fingerprints of the strong medicinal material Diangui Ainaxiang. Background technique [0002] Diangui Ainaxiang is the dried whole herb of Ainaxiang in Asteraceae, Blumea riparia (Bl)DC, and has a long history of use in Guangxi, especially in Baise. Diangui Ainaxiang is mainly produced in southwestern Guangxi (Baise, Debao, Lingyun), southern Guangdong, and southwestern to southeastern Yunnan. It has the effects of promoting blood circulation, stopping bleeding, and diuresis. Pregnancy, vaginal sores. [0003] Traditional Chinese medicine fingerprint refers to the spectrum or image of the common characteristics of each component group of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of Chinese medicinal materials or...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 蒋冰清罗盛东陈良
Owner 广西万寿堂药业有限公司
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