The invention provides an automatic sample injector, which comprises a base turntable device, a lifting device, a needle tube clamping device, a sample injection needle and a pump. The base turntable device comprises a base, a first motor and a support disk, wherein the first motor is arranged in the base and connected with the support disk. The lifting device comprises a mounting cover, a sleeve, a second motor, a lead screw and a nut. The needle tube clamping device comprises a holder and a fastener, the sample injection needle is inserted into a clamping open groove, positioned between twoarc grooves and connected with the pump through a guide tube, and the holder is fixedly arranged on a shaft of the nut. The base turntable device can be used for bearing a test tube, and the lifting device drives the needle tube clamping device to move up and down, so that the sample injection needle on the needle tube clamping device is inserted into the corresponding test tube, the pump sucks test fluid through the sample injection needle and can directly deliver the test fluid to a chromatographic analyzer for chromatographic analysis, samples are automatically injected to the chromatographic analyzer by the aid of the automatic sample injector, and chromatographic analysis efficiency is improved.