138 results about "Crustacean" patented technology

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention relates to the technical field of RNA interference, and particularly discloses an RNA interference fragment of a crustacean hyperglycemia hormone gene and application of the RNA interference fragment. The RNA interference fragment provided by the invention is injected into a third step foot of a river crab in an injection manner and is used for interfering the mRNA expression quantity of a hyperglycemia hormone gene CHH of a crustacean of the river crab. The fighting behavior of the eriocheir sinensis is effectively controlled, the yield in large-scale breeding of the eriocheir sinensis is improved, and economic losses caused by incomplete crabs due to fighting of the eriocheir sinensis are reduced.

The invention belongs to the technical field of disease prevention and treatment and aquaculture, and relates to application of acacetin in preparation of a medicine for preventing and treating white spot syndrome. The invention discloses that the acacetin has the biological activity of resisting the white spot syndrome virus for the first time, verifies that the acacetin can obviously inhibit the replication of the white spot syndrome virus in the procambarus clarkia body, and simultaneously improves the survival rate of the procambarus clarkia infected by the white spot syndrome virus. The acacetin has a good antiviral effect on the white spot syndrome virus in crustacean aquaculture, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

The invention provides a portunus trituberculatus SCP antigen, antibody, kit and application, and belongs to the technical field of allergen detection. The antigen of the portunus trituberculatus SCP comprises an antigen epitope peptide of the portunus trituberculatus SCP and a carrier protein, and the amino acid sequence of the antigen epitope peptide of the portunus trituberculatus SCP is as shown in SEQ ID NO. 14. The antigen epitope peptide of the antigen portunus trituberculatus SCP has a relatively stable structure before and after thermal processing, the detection limit of an antibody prepared from the antigen for detecting a crustacean allergen is 0.32 mu g / mL, the quantitation limit is 1.06 mu g / mL, the crustacean allergen can be specifically detected, and the antibody can be used for detecting the crustacean allergen. In addition, when the antibody is used for detecting the crustacean allergen, the accuracy is good, the matrix interference resistance and the precision are high, and the high detection effect on the crustacean allergen in food processed at high temperature can still be kept.

An incubator (1) for production of aquatic crustacean offspring (3), wherein the incubator (1) comprises: a main chamber (11); a lower collection chamber (12) which arranged below the main chamber (11). The main chamber (11) and the lower collection chamber (12) are separated by a perforated barrier (14) and are in fluid communication with each other. The main chamber (11) comprises a light source (33) configured to illuminate the main chamber (11). The main chamber (11) comprises a water distribution unit (16) configured to supply water to the main chamber (11). The incubator (1) also comprises a water outlet (21) provided in the lower collection chamber (12) which is configured to drain water from the lower collection chamber (12) in a first state (S1) of the incubator (1). The incubator (1) also comprises a biomass outlet (28) provided in the lower collection chamber (12) which is configured to drain water and aquatic crustacean offspring (3) from the lower collection chamber (12) in a second state (S2) of the incubator (1).

The present application relates to a SNP marker Ec62238 related to the reproductive traits of Exopalaemon modestus and an application thereof, and belongs to the technical field of crustacean genetics and breeding. Detection primers SEQ ID NO. 1-2 and typing primers SEQ ID NO. 3-5 are designed according to the SNP marker Ec62238. The gene sequence in which the SNP marker Ec62238 is located is shown in SEQ ID NO. 6. The base at the 601st site of the 5' end of the gene sequence is G or A. The SNP marker Ec62238 is located on the 24th chromosome of Exopalaemon modestus. The present application also provides an application of the detection primers and the typing primers in the selection of Exopalaemon modestus of good reproductive traits. The molecular marker is used for the screening of Exopalaemon modestus with high reproductive capacity or the selection of good breeds with high reproductive capacity, and can save cost and shorten the breeding period.

An electric stunning apparatus (100) for anesthetizing an aquatic animal (10), hereunder fish and crustaceans, wherein the electric stunning apparatus (100) comprises an electric stunning device (300) configured to achieve controlled exposure time for electric field strength applied to the aquatic animal (10) in the electric stunning phase. Fig. 2a.

The present invention relates to: a feed composition for zooplankton, comprising biomass derived from Schizochytrium sp. microalgae; a method for culturing zooplankton using same; and an aquaculture method for fish or crustaceans. The biomass derived from Schizochytrium sp. microalgae of the present invention has the effect of nutritionally enriching zooplankton, and when zooplankton fed with the biomass is fed to fish or crustaceans, growth of fish or crustaceans is promoted and non-specific immunity is enhanced, and thus the biomass can be effectively used as a feed composition or a feed additive.

The disclosure provides a composition comprising i) a diluent derived from a plant, yeast, insect, crustacean or algae, and ii) a nitrogen fixing microorganism; along with methods of preparing and using said composition.

The invention relates to the technical field of biological analysis, in particular to a fish and crustacean biological analysis device which comprises a placing frame and a mobile phase solvent bottle placed in the upper portion of the placing frame, a vacuum degassing box is connected to the lower portion of the placing frame, and a high-pressure pump box is connected to the lower portion of the vacuum degassing box. A sample injection box is connected to the lower part of the high-pressure pump box, a detection box is connected to the lower part of the column oven, an automatic sample injector is arranged in the sample injection box above the placing frame, and a chromatographic column is connected to the interior of the column oven. According to the biological analysis device for the fishes and the crustaceans, through slight shaking in multiple directions, the uniform shaking efficiency and effect of a sample solution in the sample solvent bottle can be improved, the situation that particles settle to the bottom of the bottle and are layered due to long-time standing of the sample solvent bottle is avoided, the concentration of the sample solution sucked by an automatic sample injector is uniform, and the accuracy of the sample solution is improved. Therefore, the accuracy of the detection result of the biological analysis device can be improved, and the biological analysis work of fishes and crustaceans cannot be influenced.

[Problem] To improve production efficiency in the breeding and farming of crustaceans.　[Solution] Provided are a method for improving the survival rate of crustaceans, a method for producing crustaceans, etc., in which a soybean-isoflavone-containing crustacean feed is fed to crustaceans. In the breeding and farming of crustaceans, a high survival rate during the breeding period can be maintained by feeding a soybean-isoflavone-containing crustacean feed to the crustaceans. Specifically, the number of deaths of crustaceans introduced into breeding and farming can be reduced, and, for example, the number of crustaceans that grow to a level suitable for shipping can be increased; therefore, the production efficiency in the breeding and farming of crustaceans can be improved.

The invention relates to the technical field of water gate cleaning, and discloses water gate cleaning equipment which comprises a mounting frame, a suspension mechanism and a cleaning mechanism. The cleaning mechanism comprises arc-shaped plates arranged at the two ends of the fixing frame, a receding groove is formed in the side, away from the suspension mechanism, of the arc-shaped plate, a fixing transverse plate is arranged in the middle of the fixing frame, a sweeping shaft is rotationally connected to the fixing transverse plate, a sweeping disc is arranged at the end of the sweeping shaft, and bristles are arranged on the side, close to the water gate, of the sweeping disc. A fixing plate is arranged on the side, away from the suspension mechanism, of the fixing frame and rotationally connected with a second driving shaft, the second driving shaft is provided with a rotating disc flush with the receding groove, a sliding sleeve is arranged on the outer side of the rotating disc and slidably connected with a sliding rod, a beating block is arranged on the side, away from the rotating disc, of the sliding rod, and the sliding rod penetrates through the receding groove. The rotating striking block is arranged to remove crustacean organisms attached to the surface of the water gate.

Uses are described for hemolymph, contents of hemolymph, fragments or components of the contents found within hemolymph, including hemocyanin, any of which may be derived from crustaceans, or recombinantly produced, as functional agents to treat diseases, provide symptomatic relief, provide a beautifying or relieving effect to the intended organ or site-of-intended-use, or be used as an ingredient in formulations designed to help regenerate, repair, or restore the function of the skin barrier, for applications falling within skin barrier restoration, wound healing and anti-aging effects.

Currently, there is no accurate method for extrapolating the coordinated oscillation system of all appendages in the fully appended multi-segmented ear-like insect structure and its appendage coordinated movement reconstruction method. In the fully appended multi-segmented ear-like insect structure, the left and right vertebrae are set along the length of the bottom trunk frame. The driving element is located at one end of the bottom trunk frame and is connected to the left and right vertebrae respectively. The first set of appendages is hinged to the outside of the left vertebrae, and the second set of appendages is hinged to the outside of the right vertebrae. The shell is fastened to the bottom trunk frame, and two shell flaps are set on the outer wall of the shell. The appendage coordinated movement reconstruction method is to extract motion feature data from the gait of existing crustaceans and arthropods, analyze and plan the appendage gait in the motion feature data, and then use a gait reconstruction mechanism to reconstruct various gaits, thereby completing the process of establishing the appendage movement model of the fully appended multi-segmented ear-like insect structure.

A process for preparing a heat-stable crustacean seafood analog product, the process comprising forming a colloidal system comprising water, non-animal protein, and an edible crosslinkable polymer; forming the colloidal system into a shape; at least partially freezing the shape at a temperature of less than or equal to 0°C for a time sufficient to achieve an at least partially frozen shape; and subjecting the at least partially frozen shape to an aqueous source of monovalent, divalent, and / or trivalent cations, wherein during step d) the edible crosslinkable polymer is ionically crosslinked with the monovalent, divalent, and / or trivalent cations to form a heat-stable shape.

The invention relates to the technical field related to medicines, in particular to a method for preventing and controlling orbiculaeas on the basis of an artemisia apiacea extract, which comprises the following specific steps: step 1, purchasing the artemisia apiacea extract from Shanghai Yuan Leaf Biotechnology Co., Ltd; step 2, separating blood egg whipstock in the economic crustacean animals to obtain an amoeboid-shaped trophozoite and microspores of the economic crustacean animals; 3, the amoeboid-shaped trophozoite is inoculated into a culture medium containing specific components to be cultured, the culture medium comprises 0.1% of mycillin, 5% of healthy portunus trituberculatus serum, 10% of fetal calf serum and 85% of NephropsSaline buffer solution, and the microspores are cultured with sterile filtered seawater. According to the method for preventing and controlling the oryza sativa based on the artemisia apiacea extracts, in-vitro experiments verify that the artemisia apiacea extracts with different concentrations (10-1000mg / L) can remarkably promote apoptosis of oryza sativa somatic cells, destroy microstructures of the oryza sativa somatic cells and inhibit physiological activity, and a new way is provided for developing novel green anti-parasitic drugs.

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

This item is a lure (worm) used for octopus fishing. It is made of a flexible material and shaped to resemble a crustacean such as a crayfish. It is used attached to a hook rig, for example, as shown in the "Reference Diagram Showing the Names of Each Part." In this example, the hook rig consists of a core rod with a fishing hook, a ball, and a worm attachment rod. This item has a hook rig attachment hole, as shown in the "AA Cross-Sectional View." Therefore, as shown in the "Reference Perspective View from the Rear Side Showing the Usage State," the worm attachment rod is passed through the hook rig attachment hole, and the hook at the tip of the worm attachment rod is hooked onto the core rod, thereby attaching the item to the hook rig. As shown in red in the "Reference Diagram Showing Air Pockets," air is trapped inside the areas corresponding to the claws and tail. Therefore, during use, buoyancy in the sea tends to cause the air pockets to face upwards. Therefore, when it lies on the seabed (with the longitudinal direction of the needle arrangement aligned with the seabed), the surface shown in the front view tends to face upwards, and the surface shown in the rear view tends to face downwards (towards the seabed). In other words, when viewed from above, the item is likely to land in the state shown in the front view. Moreover, the claws are raised, mimicking the intimidation behavior of crustaceans.

The invention discloses a method for marking strontium in enhanced and released crustacean yoke cardia bones, and relates to the field of fishery resources and environments. The method comprises the following steps: culturing the crustacean aquatic animals in a strontium-containing water body, and labeling the yoke cardia bones of the crustacean aquatic animals with strontium. According to the method, various potential target tissues of crustaceans are systematically screened, it is determined that the yoke cardia bone is the most suitable target organ marked by the strontium element for the first time, the enrichment effect of the strontium element is remarkable and stable, the marking characteristics are clear and easy to detect, and the problem that due to shell molting, a traditional marker is prone to falling off is effectively solved. Compared with a conventional method, the marking cost is greatly reduced, and the economical advantage is outstanding; and meanwhile, by virtue of the strong specificity of the element fingerprints, a release group and a natural group can be accurately distinguished, the core requirements of enhancement release on the stability and traceability of a marking technology can be met, and an efficient and reliable technical support is provided for release effect evaluation and population tracking management in crustacean wild resource restoration.

A method for producing caviar or a caviar-like product from live, mature eggs of fish or crustaceans, wherein the eggs are in a fertile but unfertilized state and have a natural potassium content in the egg plasma, includes: treating the eggs in a saline solution which does not damage the eggs and subsequently treating the live, mature eggs in a solution of water and potassium in a concentration which does not damage the eggs and does not change the eggs' potassium content. The water is deionized prior to the addition of a potassium donor for forming a cationic component in the solution, the solution has a temperature that does not damage the eggs, and the eggs are treated in the solution for a duration of a potassium exposure time until an egg envelope of the eggs obtains a desired elastic stabilization.

The invention discloses an application of a penaeus vannamei fatty acid binding protein (FABP) as an immunologic stimulant and an application of the FABP in preparation of drugs for enhancing the immune response capability of crustacean, and aims to enhance the vibrio parahaemolyticus infection resistance of the crustacean by improving the expression level of intracellular antibacterial peptides. Large-area death of crustacean caused by acute hepatopancreatic necrosis (AHPND) is avoided. The amino acid sequence number is E2IH93, the molecular weight is 15495 Dalton, no toxic or side effect exists, the synthesis cost is low, a new way is provided for treatment of crustacean bacterial infection, the ecological balance in the breeding process is not damaged, and the bacillus subtilis is an environment-friendly and efficient bacterial infection defense substance and has important application value.

The invention discloses a method for preparing crustacean aquatic product lipid based on water aqua and subcritical principle coupling, and belongs to the technical field of aquatic product processing waste / byproduct utilization. According to the method, the crustacean aquatic products and wastes / byproducts thereof are used as raw materials, a technology for preparing the lipid through drying, crushing and coupling according to an aqueous solution and a subcritical principle is constructed, and the crustacean aquatic product lipid and functional components thereof are obtained, so that the comprehensive utilization effect of the wastes / byproducts of aquatic product processing is effectively improved; and a new path is provided for high-quality and high-value utilization of the crustacean aquatic products and byproducts thereof.

The invention discloses a formula and a processing method for ecological culture of crustacean aquatic products, and the formula comprises the following medicines by weight: glycine / methionine chelated trace elements: by taking glycine as an example, not less than 13% of magnesium citrate, 9-20 g of magnesium citrate, not less than 21% of zinc glycinate, 5-9.5 g of zinc glycinate, not less than 15% of manganese glycinate, 3.5-6.5 g of manganese glycinate, not less than 15% of copper glycinate, and 1-2.5 g of copper glycinate; 6-11g of ferrous glycinate with the concentration of more than or equal to 17%; the selenium content of the yeast is 2,000 PPM, and is 1-1.5 g; per kilogram of the vitamin compound comprises 550000-8000000 IU of vitamin A, 150000-45000000 IU of vitamin D3, more than or equal to 3500 milligrams of vitamin E, more than or equal to 1200 milligrams of vitamin K3, more than or equal to 800 milligrams of vitamin B1, more than or equal to 5000 milligrams of vitamin B2, more than or equal to 800 milligrams of vitamin B6, more than or equal to 2000 micrograms of vitamin B12 and more than or equal to 15000 milligrams of vitamin C; the invention relates to the technical field of aquaculture, the formula realizes the virtuous cycle of efficient absorption, sedimentation, slow release, decomposition and supply of trace elements through artificial and natural synergistic nutrition chain design, and achieves the ecological breeding target of'zero emission 'of nutrient substances and'zero addition' of chemical agents.

Disclosed is a feed supplement for an aquatic animal comprising a dried microbial biomass. More particularly, this disclosure relates to a composition for feeding an aquatic animal comprising a first particle comprising a feed product, and a second particle consisting essentially of a dried microbial biomass, or a feed supplement for an aquatic animal comprising a dried microbial biomass in an amount of greater than about 40% w / w and the use of such composition or supplement for feeding an aquatic animal, such as a crustacean. The disclosure further relates to the use of the feed supplement for increasing the growth rate, weight and food consumption of an aquatic animal.

The invention provides a preparation method of a CTS-dsRNA nano nucleic acid pesticide for preventing and treating apple ring rot, which comprises the following steps: carrying out in-vitro synthesis and purification on dsRNA of a key pathogenic effect gene BdNIS1 of targeted silence botryosphaeria dothidea to obtain dsRNA, and adding the dsRNA into a nano chitosan colloid dispersion system to obtain the CTS-dsRNA nano nucleic acid pesticide for preventing and treating botryosphaeria dothidea. The invention further provides application for preventing and treating apple ring spot. The exposed dsRNA is combined with the nano-chitosan, the prepared CTS-dsRNA nano-nucleic acid pesticide has the effect of resisting the physalospora piricola, the expression quantity of pathogenic bacteria related target genes in the infection stage is obviously inhibited, and the infection capacity of the physalospora piricola is obviously reduced. The nano chitosan is derived from shells of crustacean organisms, is low in cost, is easy to degrade in the environment, is eco-friendly to a field, and has no residue. Therefore, the RNAi nucleic acid pesticide has a wide application prospect in the field of prevention and control of physalospora piricola.

Provided is a technique capable of simplifying the control relating to feeding and the control relating to water changing in land-based aquaculture of a crustacean. This water quality management system in land-based aquaculture of a crustacean is characterized by comprising an ammonia sensor for detecting the ammonia concentration in breeding water for the crustacean and a control unit, wherein: when the detected ammonia concentration is equal to or more than a predetermined first threshold value, the control unit reduces the amount of feeding to the crustacean compared with the case where the detected ammonia concentration is less than the first threshold value; and when the detected ammonia concentration is equal to or more than a predetermined second threshold value that is larger than the first threshold value, the control unit decides the execution of changing of the breeding water.

The invention discloses a method for synchronously preparing chitosan and citric acid through a kitchen waste lobster shell fermentation method, belongs to the field of solid waste resource utilization, and aims to solve the problems of difficult treatment, low utilization rate and pollution caused by impurity wrapping in shellfish kitchen waste treatment. According to the method, kitchen waste containing grease, chitin, calcium carbonate and protein is used as a raw material, and two-stage treatment mainly including fermentation is carried out. In the first stage, citric acid is produced through fermentation, and chitin is exposed through in-situ deoiling, decalcification and deproteinization; in the second stage, enzymolysis is mainly carried out, and after the pH is adjusted, chitinase and chitin deacetylase are exogenously added to convert chitin into chitosan; and finally, separating the feed liquid to obtain citric acid and chitosan. According to the method, fermentation is combined with enzymolysis segmented treatment, so that the problem of substrate wrapping is solved, the conversion rate and purity of chitosan are remarkably improved, high-valued co-production of waste is realized, and the process is mild and environment-friendly.

A primer set and a detection method for simultaneous qualitative and quantitative detection of EHP, DIV1, IHHNV, WSSV, and VAHPND, as well as their applications are provided, which belong to the technical field of pathogen detection. The specific sequences of the detection primer set are shown in SEQ ID NOs.1-10. A kit and detection method containing the primer set is further provided. The detection primer set and detection method exhibit strong specificity, simple and convenient operation, short detection time, high sensitivity, and enable simultaneous qualitative and quantitative detection of the five pathogens EHP, DIV1, IHHNV, WSSV, and VAHPND in samples.

The application provides application of cholesterol in preparation of a feed additive, a feed and a preparation method, and comprises the application of cholesterol or a feed-acceptable ester thereof in preparation of a feed additive for crustaceans, is used for regulating lipid metabolism of the crustaceans, and improves the antioxidant capacity of the crustaceans. The content of animal protein source in the feed is not higher than 30%. In the application, the cholesterol is added in the feed of the crustaceans as a functional additive of sterols in a low dose, on the one hand, effective addition of the cholesterol can significantly improve the growth performance of the crustaceans under the condition of low fish meal feed, on the other hand, the lipid metabolism and the antioxidant capacity of the crustaceans can be regulated, and the physiological health is maintained.