359 results about "Crustacean" patented technology

The present invention relates to feed additives for crustaceans in aquaculture, in particular crabs. The invention provides methods for making a biological compositions comprising yeast cells that can improve the immune functions of crustaceans in culture. The invention also relates to methods for manufacturing the biological compositions, and methods of using the biological compositions as feed additives.

Methods for inducing systemic, non-specific and/or sequence specific immune responses in invertebrates, e.g., marine invertebrates such as mollusks, porifera, ctenophora, echinodermas, marine worms, cnideria and preferably crustaceans, by the administration of at least one dsRNA, that confers immunity against a pathogen, or modulates expression of gene that affects growth, reproduction, and general health or "robustness" are provided. Also provided are methods of identifying invertebrate genes, e.g., crustacean genes, the expression of which is involved in the induction of non-specific (systemic) immune responses against pathogens. Also disclosed are preferred delivery systems and methods for stably administering at least one dsRNA to a crustacean whereby the dsRNA is administered via injection, immersion, in a feed or nutrient medium or comprised in a microorganism, e.g., yeast or microalgae, that expresses said dsRNA and is ingestible by said crustacean, e.g., a shrimp.

This invention is directed to compositions comprising improved edible shrimp and other crustaceans. The shrimp or other crustaceans are enriched with one or more components that provide benefits, such as improved taste and/or appearance, or improved health benefits for humans consuming them. The invention also relates to methods of producing such shrimp and other crustaceans, and methods of making a feed to feed the shrimp and other crustaceans.

The inventor has found that enzymatic treatment of crustaceans with lipolytic enzymes promotes the extraction of coloured pigment. Accordingly, the invention relates to a method for recovering coloured pigment, e.g. astaxanthin, and/or chitin or chitosan and/or lysolecithins from crustaceans. Lipolytic enzyme(s) may e.g. be lipase or phospholipase. The lipolytic enzyme treated crustacean material may be subjected to additional process steps, including treatment with non-lipolytic enzymes such as e.g. protease. The process of the invention provides several suitable products and several uses of such products. Furthermore, the invention also relates to the cosmetic and consumable products (feed and food (including beverages) products and additives produced therefrom.

The present invention relates to gastroresistant and enterosoluble microparticulate systems for the encapsulation of biologically active substances selected from: flavonoids, vitamins, antioxidants, immunostimulants, starchy and non-starchy polysaccharides, probiotics, prebiotics, intestinal trophism regulators, oligoelements, enzymes and bioactive peptides. Such microparticulate systems allow the administration of the aforementioned nutraceutic substances to animals such as porcines, bovines, caprines, ovines, equids, canids, felines, camelids, lagomorphs, rodents, fowl, and other mammals, including humans, fish and crustaceans, increasing the bioavailability.

The present invention provides a crustacean trap having a top cover and a plurality of substantially planar side panels extending downward from the cover. The top cover and the side panels define an interior space therebetween. At least one side panel may include an aperture adapted to receive a degradable element such that the degradable element substantially obstructs the aperture. The crustacean trap may also include at least one side panel having a one-way pivotable gate opening away from the interior space, with the gate having one or more protrusions spaced apart by a predetermined width.

The invention discloses a CRISPR/Cpf1 gene editing method applicable to a marine crustacean mitochondrion genome. The method includes the steps: introducing MLS signals positioned by a marine crustacean mitochondrion into a CRISPR/Cpf1 expression plasmid; designing gRNA; micro-injecting fertilized eggs; culturing the fertilized eggs in an in-vitro manner; detecting editing effect to complete editing of the marine crustacean mitochondrion genome. By the method, the marine crustacean mitochondrion genome can be directionally edited, and the method has significant value to a condition that artificial proliferation releasing markers are introduced into the marine crustacean mitochondrion genome.

Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picomavirus, such as a cricket paralysis-like picomavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.

The invention discloses a green mud crab antibacterial peptide Sphistin and application thereof, relates to the technical field of genetic engineering of crustaceans, and provides the green mud crab antibacterial peptide Sphistin and the application thereof. A molecular formula of the green mud crab antibacterial peptide Sphistin is C170H293N61O46S1 and molecular weight of the green mud crab antibacterial peptide Sphistin is 3,959.61 Dalton. The antibacterial peptide Sphistin consists of 38 amino acids, is derived from a part of sequences at end N of green mud crab histone 2A protein and is apolypeptide with an antibacterial function. By adopting the conventional solid-phase chemical synthesis method, the green mud crab antibacterial peptide Sphistin with purity of over 95 percent can beobtained. The green mud crab antibacterial peptide Sphistin has an obvious antibacterial effect on various gram negative bacteria and gram positive bacteria, has a broad antibacterial spectrum and high sterilization rate, shows extremely high medical value and is excellently applied to preparing antibacterial agents.

A composition is for feeding aquacultural prey organisms such as Artemia and rotifiers, comprising a lipid component comprising at least 25 wt % of phospholipids and providing a DHA content of at least 30 wt %. The lipid component is preferably derived from marine organisms such as fishineal, phytoplankton or zoo plankton biomass. The composition is used for providing prey organisms having a high content of highly-unsaturated fatty acids (HUFAs) suitable for aquaculture of fish including halibut, turbot, bass and crustaceans and molluses.

The invention discloses a comprehensive technology for agricultural planting and breeding, and particularly relates to a method for three-dimensional planting and breeding of agricultural products including two types of plants and one type of animal, mainly referring to a three-in-one method for three-dimensional planting and breeding of luffa, cress and crabs so as to achieve high economic benefits for high-tech agricultural planting and breeding. The method for three-dimensional planting and breeding of agricultural products like luffa, cress and crabs realizes the three-in-one three-dimensional planting and breeding of the agricultural products like luffa, cress and crabs including two types of plants, namely, luffa and cress, and one type of crustacean, namely, crabs through the building of a crab pool and a cress field, the planting and breeding of luffa and cress, and the breeding and disease prevention of the crabs. The agricultural products planted and bred by the method have low cost, high yield and high benefit, the quality of the agricultural products is greatly improved, the yield and benefit are doubled, more than ten thousand yuan per mu yield a year is realized, and the method has significant promotional value.

The invention relates to a new technology for clean production of chitosan and carboxymethyl chitosan by using crustacean shell raw materials. The method comprises the following steps: crushing shrimp shells, crab shells and other crustacean shell raw materials, directly reacting in an alcohol, alkali and water mixed system without calcium or protein removal, washing, and filtering to obtain calcium carbonate-containing crude chitosan for direct use; or directly adding chloroacetic acid, carrying out a chitosan carboxymethylation one-pot boiling reaction, washing a solid obtained after the reaction, filtering, drying to obtain a crude carboxymethyl chitosan and calcium carbonate mixture for direct use, or adding water to dissolve the mixture to obtain separated calcium carbonate which can be used a filler and a solution which can be used as a product directly or after concentration drying, and carrying out alcohol precipitation to obtain pure carboxymethyl chitosan. The above crude or pure product can be widely used in the fields of papermaking, binders, coatings and sustained and controlled release fertilizers. A washed filtrate contains amino acids and small peptides, and can be used as an animal amino acid fertilizer raw material or plant amino acid fertilizer or microbial nitrogen source nutriment after solvent concentration recovery.

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

An animal feed mixture containing DL-methionyl-DL-methionine and salts thereof for animals kept in aquacultures is provided. Methods for preparing DL-methionyl-DL-methionine of formula (I)

and methods to fractionate the diasteriomeric forms obtained are also provided.

An isolated strain of bacteriophage, specific against bacteria belonging to the Vibrio genre, particularly the anguillarum species, deposited on 3 Oct. 2012 at the Polish Collection of Microorganisms (PCM) of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, with access number F/00072, characterized in that said strain is efficient in the prophylaxis, control and/or treatment of the infection caused by Vibrio anguillarum in all types of species of fish, mollusks and crustaceans that are important for aquaculture susceptible to this bacteria, genome size 48.6 Kb, it is not sensitive to chloroform and its storage temperature is −80° C.

The invention provides a production method for purified recombinant proteins of commercial value prepared in enclosed aquatic systems. The production system provides biosecure containment of genetically engineered aquatic organisms for an alternative production method for complex proteins requiring post-translational modification that is best done in eukaryotic expression systems. Possible aquatic organisms that can be utilized by this invention are crustaceans (e.g. penaeid shrimp or Artemia) and teleost fish (e.g. trout or tilapia).

A method for directing the displacement of aquatic crustaceans after feeding by utilizing their biological tendency responding to a bright and dark contrast is provided. This method comprises steps to generate the visual effect of this bright and dark contrast at a predetermined place within the water body of culture. Light coming from light emitters directly or from reflection of protrusions and/or painted patterns/figures, and/or filtration of light filters, with the existence of illuminating light, are used for generating visual effect of the bright and dark contrast.

A method for determining the presence of delayed food allergy and intolerance against antigens extracted from modified foods. The method includes determining a level of antibodies against a modified dietary food antigen in blood and mucosal samples from the patient and comparing the level with normal levels of the antibodies. Dietary antigens that were tested include milk and modified milk products; eggs and modified egg products; meat and modified meat products; fish, mollusks, and crustaceans and their modified products; oils, fats and their modified products; grains and modified grain products; pulses, seeds kernels, nuts and their modified products; vegetables and modified vegetable products; fruits and modified fruit products; sugar, modified sugar products, modified chocolate products and confectionery; and spices and their modified forms.

A method of enhancing aquatic organism growth is provided. The method comprises the step of introducing an alkane into water containing the organism. The alkane can be introduced intermittently, either alone or with another gas such as oxygen. Nutrients can be introduced with the alkane or separately. The alkane can comprise a butane substrate and can include at least one of n-butane and iso-butane. The organism can be selected from the group of: fish, crustaceans, mollusks, algae and aquatic plants. Organisms grown by the method and systems for practicing the method are also included.

In accordance with at least one aspect of this disclosure, a crustacean trap can include a cage configured to trap one or more crustaceans, and a propulsion system connected to the cage and configured to provide propulsion to the cage. In certain embodiments, the trap can include a controller configured to control the propulsion system to autonomously pilot the trap.

A device for cultivating and maintaining aquatic crustaceans and the method for the same are provided, whereby aquatic crustaceans are cultured in a culture tank having at least one effective resting and/or hiding area that provides visual only or both visual and tactile sensations for aquatic crustaceans. The present invention creates effective resting and/or hiding areas by utilizing the sensitivity of aquatic crustaceans to physical environment, thereby allowing aquatic crustaceans to live within confined spaces in high densities without cannibalizing those fall outside the effective resting and/or hiding areas (the non-effective habitat areas). The device of the present invention can cultivate and maintain aquatic crustaceans successfully in shallow waters and a plurality of which may be vertically incorporated into a multi-layered culture system to bring about higher culture and maintenance densities per unit floor area.

A method for in situ bioremediation of contaminants in the environment is described. The method includes adding an electron donor to ground water in an amount sufficient for a microbe in the ground water to use the electron donor for reducing the contaminant into an innocuous derivative thereof. Illustratively, the electron donor contains about 0.1 to 75% by weight of chitin, such as crustacean shell, partially deproteinized crustacean shell, ground mushrooms, or a fungal fermentation broth. The chitinous electron donor can be added to the ground water as a particulate solid or aqueous slurry.

The present invention contemplates the creation of a low fluoride oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO₂ and ethanol as solvents, inter alia krill oil is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material. The products have low fluoride content. The manufacturing costs in the extraction process are relatively low.