10 results about "Procambarus clarkii" patented technology

The application discloses a kind of based on water temperature illumination coupling regulation and control procambarus clarkii ovulation induction system, for the existing system There are many environmental factors, poor coordination ability, temperature monitoring precision is insufficient, lack of real-time perception of physiological state of shrimp problem.The system of the present application includes heat insulation workshop, induced ovulation pool, high-level reservoir, water temperature control module, illumination control module, oxygenation module, water quality monitoring module, microporous camera, dehumidification device, bottom improvement agent automatic feeding device, non-contact yolk protein fluorescence detection probe and intelligent auxiliary system, intelligent auxiliary system is connected with each module respectively Unified control.The present application realizes the time sequence coupling regulation and control of multiple factors such as water temperature, illumination, water level and humidity by coordinating each module through intelligent auxiliary system, and effectively improves the ovulation induction success rate and synchronism of procambarus clarkii by real-time perception of physiological state of shrimp.The present application is mainly used for artificial propagation and seedling scale production of procambarus clarkii.

The present application relates to a kind of anti-procambarus clarkii white spot syndrome virus monoclonal antibody and its application, the complementary determining region CDR1-3 sequence of the heavy chain variable region of the anti-procambarus clarkii white spot syndrome virus monoclonal antibody is sequentially shown as SEQ ID NO:3-5, the complementary determining region CDR1-3 sequence of the light chain variable region is sequentially shown as SEQ ID NO:6-8.The present application successfully obtains the high affinity of anti-VP28 monoclonal antibody, and it has excellent affinity to procambarus clarkii white spot syndrome virus.The monoclonal antibody can be used for specific detection to procambarus clarkii white spot syndrome virus, and has good virus neutralization activity.By adding the monoclonal antibody in water body, the resistance of procambarus clarkii to white spot syndrome virus can be greatly improved, easy to operate, without injection, suitable for large-scale breeding scene, provide a new technical means for the prevention and control of white spot syndrome virus disease in aquaculture.

The present disclosure provides a method and system for identifying individual procambarus clarkii of biological characteristics, relating to the technical field of deep learning, comprising: extracting local and global digital biological characteristics of each slice image and a target image, and fusing the local and global digital biological characteristics to obtain a digital biological characteristic matrix; inputting the digital biological characteristic matrix into an improved graph neural network model, generating a query and a key through an independent multi-layer perceptron, calculating edge weights through a scaling dot product of the query-key, and obtaining multiple node features after multi-head attention splicing; splicing different node features, generating an attention score for each node based on a gating mechanism aggregation module, and outputting a global representation vector of each group of point sets after global pooling operation; mapping the global representation vector to a fixed dimension through a linear network, and taking it as a final point set embedding, and finally outputting a procambarus clarkii individual identification result.

The application belongs to the technical field of biomolecular markers, and particularly relates to a primer set for detecting a SNP molecular marker significantly related to the fecundity trait of Procambarus clarkii and application thereof. The primer set can quickly and effectively realize accurate genotyping of a SNP site of a vitellogenin Vg gene of the Procambarus clarkii, and the detection can be completed only by using conventional PCR and agarose gel electrophoresis, so that the detection process is simple, time-consuming is short, and cost is low, and the primer set is suitable for large-scale and high-throughput parent screening. The marker is significantly related to the fecundity traits such as clutch size of the Procambarus clarkii, the genotyping result is stable and reliable and has good repeatability, only the end of the tail fan needs to be sampled, dissection is not needed, and the genotype identification can be completed in the early stage of breeding, so that the selection and breeding cycle is greatly shortened, the high-fecundity parent screening efficiency is improved, the problems such as long selection and breeding cycle and low efficiency in traditional phenotype selection and breeding are effectively solved, a stable and practical molecular tool is provided for the genetic improvement and efficient breeding of the Procambarus clarkii, and the application has important industrial application value.

The present application belongs to the technical field of red claw crayfish breeding, and particularly relates to a fermented feed for improving the quality and immunity of red claw crayfish and a preparation method thereof. The fermented feed for improving the quality and immunity of red claw crayfish is prepared by fermenting the following raw materials in parts by weight: 15-25 parts of imported fish meal, 12-20 parts of fermented soybean meal, 5-8 parts of shrimp shell powder, 3-6 parts of spirulina powder, 5-10 parts of corn gluten meal, 10-15 parts of high-gluten flour, 3-5 parts of yeast extract, 2-4 parts of soybean phospholipid, 3-5 parts of fish oil, 1-2 parts of calcium dihydrogen phosphate, 1-2 parts of multi-vitamin and multi-mineral, 0.1-0.3 parts of beta-glucan, 2-5 parts of compound probiotic fermentation agent, and 32-40 parts of water. The compound probiotic fermentation agent is prepared by mixing Haematococcus pluvialis, Bacillus circulans and marine Rhodotorula in a mass ratio of 3-5:3-4:2-3. The fermented feed of the present application can significantly improve the body color, muscle quality and immunity of the crayfish.

The application discloses a SNP molecular marker related to a Procambarus clarkii meat yield character and application thereof, and relates to the following steps: genotyping P. clarkii through whole genome association analysis, performing whole genome association analysis on the meat yield of P. clarkii, and screening out SNP markers significantly related to the meat yield character, including one or more of SNPs 1-SNPs 10. In the application, the P. clarkii with high growth speed and high meat yield can be effectively predicted and selected by detecting the above-mentioned SNP markers, the selection efficiency and accuracy of the P. clarkii are significantly improved, and a new P. clarkii variety with high yield and high quality is selected, so that the application has a wide application prospect in the molecular breeding of the meat yield character of the P. clarkii.

The present application relates to red chelae crayfish catching technical field, especially a kind of red chelae crayfish paddy field cultivation is used to catch shrimping device and method, including the laser lifting device, guide device and collection escape-proof device sequentially arranged along vertical direction, laser lifting device is inducted shrimping group by the green laser aperture of liftable adjustment;Guide device uses the four-prism platform gentle slope and U-shaped tube water flow with fishing net cover, guide shrimping group to climb, and make shrimps fall into collection area by the flipper triggered by shrimp body self-weight;Collection escape-proof device realizes no-escape collection by escape-proof board, one-way collection net cage and inverted whisk structure, the present application utilizes the photophobicity and top water habit of red chelae crayfish, realizes the full-process automation closed loop operation from "induction, guide, fall shrimps to collection", effectively solves the problems of traditional ground cage fishing, such as high labor intensity, low efficiency, high damage and serious escape, has the advantages of high efficiency, low damage and strong adaptability.

The application discloses a constant-temperature compartmentalized procambarus clarkii clutch egg hatching device, and belongs to the technical field of aquaculture. In view of the problems of low hatching rate caused by the mutual interference of parents and the mechanical damage of fertilized eggs in the traditional hatching process, the device comprises a hatching pool with a smooth bottom, a water inlet is arranged on one side of the hatching pool, and an inner plug pipe overflow outlet capable of being pulled up and inserted is arranged at the bottom of the other end of the hatching pool; at least one procambarus clarkii clutch egg compartment module is arranged in the hatching pool, a plurality of cylindrical compartments are arranged in the module, a passageway is arranged between every two rows of compartments, four openings in a cross four-end position are formed in the bottom of each compartment, the compartments, the adjacent compartments and the adjacent passageways are connected with each other through the openings, and the openings allow water to flow but prevent clutch female crayfish from passing; and a constant-temperature system comprising a heating element and a temperature sensor, the heating element is automatically started and stopped according to real-time data of the temperature sensor. The device is mainly used for the industrial hatching of procambarus clarkii fertilized eggs.