62 results about "Procambarus clarkii" patented technology

The utility model discloses an artificial breeding nest structure for breeding crayfishes in sandy soil in northwest arid regions, which is suitable for sandy soil water environment in northwest arid regions, and uses PVC (polyvinyl chloride) pipes as breeding pipes of the crayfishes, so that durability and corrosion resistance of a breeding device are guaranteed, and the breeding pipes have good light resistance and heat preservation. Circulating holes are further formed in the breeding pipes and used for helping water in the pipes to normally circulate, circulation of water bodies is ensured, water in the breeding pipes continuously flows, dissolved oxygen in the pipes is increased, a more suitable growth environment is created for the crayfishes, meanwhile, during breeding of the crayfishes, separated crayfish eggs can be collected through the breeding net, and the breeding efficiency is improved. The crayfish can be prevented from escaping all around, so that the economic loss is avoided; the product effectively solves the problems of a traditional nest in a sandy soil water body, improves the breeding efficiency and survival rate of the crayfish, and improves the production economic benefits of crayfish farmers in the northwest arid region to a certain extent.

The invention belongs to the technical field of disease prevention and treatment and aquaculture, and relates to application of acacetin in preparation of a medicine for preventing and treating white spot syndrome. The invention discloses that the acacetin has the biological activity of resisting the white spot syndrome virus for the first time, verifies that the acacetin can obviously inhibit the replication of the white spot syndrome virus in the procambarus clarkia body, and simultaneously improves the survival rate of the procambarus clarkia infected by the white spot syndrome virus. The acacetin has a good antiviral effect on the white spot syndrome virus in crustacean aquaculture, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

The application discloses a SNP molecular marker for identifying the genetic sex of Procambarus clarkii, wherein the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No. 1, and a male individual is shown as GG homozygote at the 43th base position of the nucleotide sequence of the SNP molecular marker, and a female individual is shown as GA heterozygote at the 43th base position of the nucleotide sequence of the SNP marker. The application further discloses primers and kits for detecting the SNP molecular marker, and application of the reagents, primers and kits for detecting the SNP molecular marker in genetic sex identification of the Procambarus clarkii. The SNP marker is closely related to the genetic sex, and can be used for conveniently and quickly identifying the genetic sex of the Procambarus clarkii in the process of family breeding and unisexual breeding of the Procambarus clarkii, and provides a theoretical basis for identification of sex-reversed individuals in the breeding process.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The utility model provides a crayfish cleaning machine, and relates to the technical field of crayfish cleaning equipment, in order to solve the problem that in the prior art, a crayfish cleaning machine is poor in cleaning effect on dirt on the surfaces of crayfishes, and the crayfish cleaning machine comprises a machine box, an upper rolling brush assembly and a lower rolling brush assembly. The upper rolling brush assembly and the lower rolling brush assembly are both rotatably connected into the machine box, the upper rolling brush assembly is correspondingly arranged on the upper side of the lower rolling brush assembly, a gap for lobsters to pass through is formed between the upper rolling brush assembly and the lower rolling brush assembly, an inlet is formed in one end of the machine box, and a delivery outlet is formed in the other end of the machine box. The rotation direction of the upper rolling brush assembly can be the same as or opposite to that of the lower rolling brush assembly, and the crayfish can pass through the gap from the inlet and then be conveyed to the outlet. The crayfish cleaning machine simulates the action of manual repeated scrubbing, the scrubbing effect on crayfishes is good, manual cleaning is replaced, the manual cleaning effect can be achieved, and the cleaning efficiency is improved.

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The invention belongs to the field of molecular marker screening of aquatic animals, and provides a KASP molecular marker for identifying high temperature resistance of procambarus clarkii and application of the KASP molecular marker. According to the procambarus clarkia high-temperature-resistant gene Hsp70-1 and the procambarus clarkia high-temperature-resistant gene Hsp70-2 disclosed by the invention, two SNP (Single Nucleotide Polymorphism) sites of the procambarus clarkia high-temperature-resistant gene Hsp70-1 and the procambarus clarkia high-temperature-resistant gene Hsp70-2 are remarkably related to high-temperature resistance, Hsp70-1-Hap1 haplotypes and Hsp70-2-Hap1 haplotypes are high-temperature-resistant dominant haplotypes, and the application potential of high-temperature-resistant application through combination of the double genes of KSAP markers developed aiming at the loci are good in typing effect, high in specificity, good in stability, convenient to detect, economical and efficient, and can be widely applied to breeding of high-temperature-resistant varieties of the procambarus clarkii. The KASP marker disclosed by the invention can screen out the procambarus clarkii with the SNP haplotype combination with the high-temperature-resistant advantage, and meets the technical requirements of high-temperature-resistant molecule-assisted breeding of the procambarus clarkii.

The present application provides a kind of shrimp shell biomass charcoal prepared by composite preparation and its preparation method and application, the present application is first prepared and obtained the composite preparation containing chitinase, bacillus licheniformis and bacillus subtilis, the chitinase is the chitinase derived from hartz wood enzyme is mutated, constructs mutant library, with trichoderma reesei as expression host, by screening, the excellent mutant Chi M1 obtained, its chitinase activity is significantly improved, is 2 times of original gene. By spraying the fermentation broth of the recombinant bacteria of the present application to obtain enzyme spray powder and the compounding of bacillus licheniformis powder and bacillus subtilis powder, the degradation efficiency of procambarus clarkia shrimp shell can be effectively improved. The composite preparation containing ChiM1 enzyme spray powder has good application prospect in the fields of aquaculture, feed additive and biomass resource utilization.

The application discloses a kind of based on water temperature illumination coupling regulation and control procambarus clarkii ovulation induction system, for the existing system There are many environmental factors, poor coordination ability, temperature monitoring precision is insufficient, lack of real-time perception of physiological state of shrimp problem.The system of the present application includes heat insulation workshop, induced ovulation pool, high-level reservoir, water temperature control module, illumination control module, oxygenation module, water quality monitoring module, microporous camera, dehumidification device, bottom improvement agent automatic feeding device, non-contact yolk protein fluorescence detection probe and intelligent auxiliary system, intelligent auxiliary system is connected with each module respectively Unified control.The present application realizes the time sequence coupling regulation and control of multiple factors such as water temperature, illumination, water level and humidity by coordinating each module through intelligent auxiliary system, and effectively improves the ovulation induction success rate and synchronism of procambarus clarkii by real-time perception of physiological state of shrimp.The present application is mainly used for artificial propagation and seedling scale production of procambarus clarkii.

The application discloses a SNP molecular marker related to a white spot syndrome virus (WSSV) tolerance trait of Procambarus clarkii, which is located in a 3rd intron region of a TRIM gene, specifically, a T>C type mutation at 585bp of a DNA sequence shown in SEQ ID NO. 1, wherein a T / C heterozygous genotype has significant correlation with the WSSV tolerance trait of the Procambarus clarkii. The application provides a favorable marker for molecular selection of the Procambarus clarkii, and can accelerate the selection process of the disease-resistant variety of the Procambarus clarkii.

The application belongs to the technical field of biology and particularly relates to a functional recombinant Cas9 protein targeted to an ovocyte of Procambarus clarkii and application thereof. The protein is NLs-VgSP-Cas9-NLs. The application directly delivers the recombinant Cas9 protein, thereby reducing the risk of integration of exogenous genes. By specifically targeting the ovocyte, off-target effects on somatic cells can be avoided, the efficiency of editing of germ cells is improved, and early development or genetic manipulation can be conveniently studied. The ovocyte of Procambarus clarkii is large, the recombinant Cas9 protein is efficiently delivered by receptor-mediated endocytosis on the surface of the ovocyte, and the problem that microinjection of zygotes cannot be implemented is solved.

The utility model discloses a cold-region crayfish seed transportation device, and belongs to the technical field of aquatic product transportation. The technical problem that the survival rate is low in the long-distance dry-method crayfish fry transportation process in northern cold regions is solved. A cold-region crayfish seed transporting device comprises a heat preservation box, a crayfish frame, an air pump, an oxygen pipe, an aeration disc and other core assemblies. A shrimp cavity is formed in the heat preservation box, the aeration disc is arranged at the bottom of the shrimp cavity in the horizontal direction, and the air pump is arranged on one side of the heat preservation box. An opening, an upper cover and a pipe orifice are arranged on the top surface of the heat preservation box, one side of the upper cover is hinged to one side of the opening, one end of the oxygen pipe penetrates through the pipe orifice to be connected with the aeration disc, and the other end of the oxygen pipe is connected with the air pump; the multi-layer shrimp frame is placed on the upper side of the aeration disc and comprises a frame and a shrimp net, the shrimp net surrounds the surface of the frame, a net opening and a net cover are arranged on the upper surface of the shrimp net, and one side of the net cover is hinged to one side of the net opening. The transportation survival rate of the young crayfish in the cold region is improved, and the method is used for long-distance transportation of the young crayfish.

The present application relates to a kind of anti-procambarus clarkii white spot syndrome virus monoclonal antibody and its application, the complementary determining region CDR1-3 sequence of the heavy chain variable region of the anti-procambarus clarkii white spot syndrome virus monoclonal antibody is sequentially shown as SEQ ID NO:3-5, the complementary determining region CDR1-3 sequence of the light chain variable region is sequentially shown as SEQ ID NO:6-8.The present application successfully obtains the high affinity of anti-VP28 monoclonal antibody, and it has excellent affinity to procambarus clarkii white spot syndrome virus.The monoclonal antibody can be used for specific detection to procambarus clarkii white spot syndrome virus, and has good virus neutralization activity.By adding the monoclonal antibody in water body, the resistance of procambarus clarkii to white spot syndrome virus can be greatly improved, easy to operate, without injection, suitable for large-scale breeding scene, provide a new technical means for the prevention and control of white spot syndrome virus disease in aquaculture.

The invention discloses an ecological rice planting method for crop rotation of crayfish and macrobrachium rosenbergii, and belongs to the technical field of agricultural production. The invention solves the problem of how to further improve the effect of rice-shrimp co-culture and fully exert the advantages in the prior art. The method comprises the following steps: S1, planting rice in a rice field from April to May every year; s2, after rice tillering and the water temperature is constant to 22 DEG C or above, macrobrachium rosenbergii seeds are put in; s3, starting to catch macrobrachium rosenbergii from August to September every year; s4, harvesting the rice; s5, planting aquatic plants; s6, putting young crayfish in October; and S7, catching the crayfishes in March of the next year, and then repeating the steps S1-S7. According to the method, on the basis of original one-season rice and one-season shrimps, one-season shrimp breeding is added, and the unit output value and benefits of the rice field are increased. By reasonably regulating and controlling the cultivation time of the macrobrachium rosenbergii, the crayfish and the rice, the marketing time of the macrobrachium rosenbergii and the crayfish is advanced by one month, the economic value of the rice field is fully volatilized, and the produced economic value is higher.

The application discloses a device for breeding good species of crayfish and application thereof, and the device comprises a population selection unit and a family selection unit; the population selection unit is a cuboid in overall structure, and comprises a population selection water tank, a population selection crayfish tank and a crayfish nest; the population selection crayfish tank is detachably embedded in the population selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the population selection crayfish tank and the population selection water tank; the family selection unit is a cylinder in overall structure, and comprises a family selection water tank, a family selection crayfish tank and a crayfish nest; the family selection crayfish tank is detachably embedded in the family selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the family selection crayfish tank and the family selection water tank. Advantages of the device are as follows: the device utilizes the self behavior characteristics and external nest position advantages of crayfish to first breed parent individuals with strong bodies and strong resistance, and then remove the nest position advantages to breed individuals with strong reproductive capacity; and the device can obtain crayfish parents with stable genetic traits.

The invention provides a procambarus clarkii individual identification method and system based on biological characteristics, and relates to the technical field of deep learning. Extracting local digital biological characteristics of each slice image and the target image and global digital biological characteristics of the target image, and fusing the local digital biological characteristics and the global digital biological characteristics to obtain a digital biological characteristic matrix; inputting the digital biological characteristic matrix into an improved graph neural network model, respectively generating a query and key through an independent multi-layer perceptron, calculating an edge weight through a zoom dot product of the query and key, and carrying out multi-head attention splicing to obtain a plurality of node characteristics; splicing different node features, generating an attention score for each node based on an aggregation module of a gating mechanism, and outputting a global representation vector of each group of point sets after global pooling operation; and mapping the global representation vector into a fixed dimension through a linear network, embedding the fixed dimension as a final point set, and finally outputting to obtain a procambarus clarkii individual identification result.

The present disclosure provides a method and system for identifying individual procambarus clarkii of biological characteristics, relating to the technical field of deep learning, comprising: extracting local and global digital biological characteristics of each slice image and a target image, and fusing the local and global digital biological characteristics to obtain a digital biological characteristic matrix; inputting the digital biological characteristic matrix into an improved graph neural network model, generating a query and a key through an independent multi-layer perceptron, calculating edge weights through a scaling dot product of the query-key, and obtaining multiple node features after multi-head attention splicing; splicing different node features, generating an attention score for each node based on a gating mechanism aggregation module, and outputting a global representation vector of each group of point sets after global pooling operation; mapping the global representation vector to a fixed dimension through a linear network, and taking it as a final point set embedding, and finally outputting a procambarus clarkii individual identification result.

The invention relates to the technical field of image processing, in particular to an intelligent grading method for procambarus clarkii based on an image processing technology. The method comprises the following steps: acquiring a back trunk area and a side trunk area in a back image and a side image of procambarus clarkii; obtaining the contour edge of the back torso area and edge segments in the back torso area, and screening the edge segments to obtain suspected abdominal segment edge segments; the suspected abdominal segment edge section closest to the tail direction and the contour edge of the back trunk area form a suspected tail segment area, then whether the shrimp tail is curled or not is judged, and if yes, a first key point and a second key point are obtained; thirdly, acquiring a third key point and a fourth key point on the contour edge of the side trunk area; and analyzing the edge section between the third key point and the fourth key point to obtain the curling degree of the shrimp tail, further correcting the area of the back trunk area, and judging the grade of the procambarus clarkii according to the corrected area. According to the method, the grading accuracy of the procambarus clarkii can be improved.

The invention discloses development and application of a heat shock protein 70 molecular marker related to cold resistance characters of procambarus clarkii, relates to the technical field of molecular biology, and aims to solve the problem of lack of molecular markers for identifying or screening cold-resistant procambarus clarkii in the prior art. According to the technical key points, the molecular marker related to the cold resistance character of the procambarus clarkii is provided and comprises a molecular marker A and a molecular marker B. The molecular marker A is located at the 555bp site of a nucleotide sequence shown in SEQ ID NO.1, and the basic group of the site is C or T; the molecular marker B is located at the 964bp site of the nucleotide sequence shown in SEQ ID NO. 1, and the basic group of the site is A or C. The molecular marker provided by the invention has a wide application prospect in identification or screening of cold-resistant procambarus clarkia.

The present application relates to a kind of anti-procambarus clarkii white spot syndrome virus monoclonal antibody and its application, the complementary determining region CDR1-3 sequence of the heavy chain variable region of the anti-procambarus clarkii white spot syndrome virus monoclonal antibody is sequentially shown as SEQ ID NO:3-5, the complementary determining region CDR1-3 sequence of the light chain variable region is sequentially shown as SEQ ID NO:6-8.The present application successfully obtains the high affinity of anti-VP28 monoclonal antibody, and it has excellent affinity to procambarus clarkii white spot syndrome virus.The monoclonal antibody can be used for specific detection to procambarus clarkii white spot syndrome virus, and has good virus neutralization activity.By adding the monoclonal antibody in water body, the resistance of procambarus clarkii to white spot syndrome virus can be greatly improved, easy to operate, without injection, suitable for large-scale breeding scene, provide a new technical means for the prevention and control of white spot syndrome virus disease in aquaculture.

The invention provides an SNP molecular marker related to the meat percentage of procambarus clarkii and application of the SNP molecular marker, and belongs to the technical field of genetic breeding of procambarus clarkii. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the sequences of the primers of the SNP molecular marker are as shown in SEQ ID NO.2 and SEQ ID NO.3, and the meat yield of the procambarus clarkii can be increased by performing auxiliary breeding on a breeding population through the SNP molecular marker and the primers thereof, so that the economic value of the procambarus clarkii is increased.

The invention relates to the technical field of aquaculture environment control, and discloses a cold region crayfish hatchery Internet of Things intelligent regulation and control system, which comprises a full-automatic hatching system, and is characterized in that the full-automatic hatching system comprises a hatching unit, an environment regulation module, an identification module, a decision module and an execution unit; the hatching unit divides the crayfish hatching farm into a plurality of groups of hatching sub-units with the same area, and the interiors of the hatching sub-units are provided with the same quantity of crayfish fertilized eggs. The execution unit regulates and controls the heater, the refrigerator, the humidifier and the dehumidifier according to control instructions (first / second / third decisions and abnormal instructions) generated by the decision module and triggers the early warning device to send early warning information to workers in an abnormal state, and an intelligent management and control system with full-process automation and manual intervention cooperation is formed. The hatching success rate and the breeding efficiency are obviously improved.

The application discloses a kind of northwest arid region sandy soil small crayfish breeding artificial breeding nest manufacturing method, including artificial breeding nest is formed by the stacking of multiple breeding devices, breeding device includes breeding pipe, the entrance of small crayfish is opened in the side of breeding pipe, and strip-shaped through-hole and multiple flow-through holes are provided in the length direction of breeding pipe;At least two breeding pipes are oppositely arranged, and the two ends of breeding net respectively pass through the strip-shaped through-hole of two breeding pipes, and the breeding net and breeding pipe are fixed by fixing belt;Small crayfish breeding nest is formed by stacking multiple breeding devices;Breeding device is simple to make, and production cost is low, and the structural design of breeding device is suitable for the water environment in northwest arid region, to bring good living environment for small crayfish, will not cause pollution to water environment, can create certain economic benefits for small crayfish breeder;The water retention and stability of the breeding device of the application effectively solve the problems faced by traditional nest in sandy water.

The utility model relates to a head-body ratio measuring device for aquatic animals, which comprises a workbench, a measuring scale is arranged on the workbench, and the measuring scale comprises a fixed arm and a rotating arm; the device further comprises a light generation assembly, the light generation assembly comprises a fixed light source and a movable light source, the fixed light source is arranged on the fixed arm, the light generation assembly has horizontal and vertical adjusting capacity, light emitted by the fixed light source and the movable light source is staggered on the aquatic animals to be tested, and measurable included angle light is formed on the workbench. The head-to-body ratio measuring device is suitable for measuring the head-to-body ratio of various aquatic animals, and is suitable for measuring the head-to-body ratio of aquatic animals with different thicknesses, such as procambarus clarkii, bighead carps and the like, in different sizes by adjusting the height of the movable measuring line.

The application discloses an antibacterial and antiviral polypeptide Litomusin for white shrimp 81‑100 and application thereof, the molecular formula of the polypeptide is C 106 H 183 N 35 O 25 S1, and the amino acid sequence of the polypeptide is shown as SEQ ID NO. 01. The polypeptide has good water solubility, has significant antibacterial activity on various bacteria and fungi, has good antibacterial effect and high efficiency, has a wide antibacterial spectrum, has strong activity on white spot syndrome virus, has no toxicity on hematopoietic tissue (Hpt) cells of Procambarus clarkii, and has a good application prospect in drug research and development.

The utility model relates to a facility aquaculture system for cherax quadricarinatus, which comprises a long-strip-shaped pond body arranged on the ground and comprises a fence support and waterproof cloth arranged on the inner side of the fence support. The length direction of the cherax quadricarinatus facility cultivation system is consistent with the length direction of the pond body, the width direction of the cherax quadricarinatus facility cultivation system is consistent with the width direction of the pond body, and the height direction of the cherax quadricarinatus facility cultivation system is consistent with the height direction of the pond body; the water inlet is formed in the first end of the tank body in the length direction; the water outlet is formed in the second end, opposite to the first end, of the tank body; the hiding room is cylindrical and is arranged in the pool body, and the axis of the hiding room extends in the width direction; the hiding houses are arranged on the two sides of the pool body in the width direction in an array mode in the length direction. After drainage, the pool body enclosed by the waterproof cloth can be conveniently subjected to sunlight disinfection, the disinfection cost is low, the disinfection is thorough, and the efficiency is high. A large number of arrayed hiding houses can allow a large number of cherax quadricarinatus to inhabit.

The invention discloses feed mixing and processing equipment for crayfish breeding and a processing method, and relates to the technical field of feed mixing and processing.The equipment comprises a tank body, a discharging pipe is installed at the lower end of the side wall of the tank body, a supporting frame is installed at the lower end of the tank body, and a first motor is vertically installed in the center of the bottom of the supporting frame; a first rotating rod is mounted at the output end of the first motor, the upper end of the first rotating rod penetrates through the bottom of the tank body and is arranged in the tank body, a plurality of lifting plates are horizontally mounted on the side wall, close to the bottom of the tank body, of the first rotating rod, and a feeding mechanism is arranged at the upper end of the tank body. According to the device, powder is blown into the tank body and flows on the right-cone-shaped stop block, the inverted-cone-shaped collecting frame, the fixing barrel and the spiral blades, then all the powder can be preliminarily mixed, after the powder is blown out through the fixing barrel, water is sprayed to the powder through the spraying head, the powder can be wetted, and caking and clustering of feed are reduced.

The application belongs to the technical field of biomolecular markers, and particularly relates to a primer set for detecting a SNP molecular marker significantly related to the fecundity trait of Procambarus clarkii and application thereof. The primer set can quickly and effectively realize accurate genotyping of a SNP site of a vitellogenin Vg gene of the Procambarus clarkii, and the detection can be completed only by using conventional PCR and agarose gel electrophoresis, so that the detection process is simple, time-consuming is short, and cost is low, and the primer set is suitable for large-scale and high-throughput parent screening. The marker is significantly related to the fecundity traits such as clutch size of the Procambarus clarkii, the genotyping result is stable and reliable and has good repeatability, only the end of the tail fan needs to be sampled, dissection is not needed, and the genotype identification can be completed in the early stage of breeding, so that the selection and breeding cycle is greatly shortened, the high-fecundity parent screening efficiency is improved, the problems such as long selection and breeding cycle and low efficiency in traditional phenotype selection and breeding are effectively solved, a stable and practical molecular tool is provided for the genetic improvement and efficient breeding of the Procambarus clarkii, and the application has important industrial application value.

The invention relates to the technical field of ecological breeding, in particular to a rice, shrimp and eel breeding method which comprises the following steps: S1, rice field preparation: digging a circular groove at a position of 100-200 cm in a rice field along the inner edge of a ridge of the rice field, the depth of the groove being 100-120 cm, the area of the circular groove being less than 10% of the total area of the rice field, and digging four-side circular grooves or special-shaped grooves according to local conditions; the procambarus clarkii is mainly bred from late March to early June, rice seedlings are transplanted in mid June, ricefield eels are put in the late June, rice, ricefield eels and shrimps are co-bred from late June to October, the period from the mid October to March of the next year is the ricefield eel quality improvement time period, and the ricefield eels are caught and sold; the use amount of pesticide and chemical fertilizer for rice is reduced, no pollution is caused to the surrounding environment, the produced shrimps and eels are larger in specification, harmonious and high-quality shrimp, rice and eel planting and breeding space is large, the technical replicability is high, popularization is easy, cost is low, the effect is fast, the risk is small, the scale can be large or small, flexibility and easy control are achieved, the investment can be large or small, and elasticity is high.

The present application belongs to the technical field of red claw crayfish breeding, and particularly relates to a fermented feed for improving the quality and immunity of red claw crayfish and a preparation method thereof. The fermented feed for improving the quality and immunity of red claw crayfish is prepared by fermenting the following raw materials in parts by weight: 15-25 parts of imported fish meal, 12-20 parts of fermented soybean meal, 5-8 parts of shrimp shell powder, 3-6 parts of spirulina powder, 5-10 parts of corn gluten meal, 10-15 parts of high-gluten flour, 3-5 parts of yeast extract, 2-4 parts of soybean phospholipid, 3-5 parts of fish oil, 1-2 parts of calcium dihydrogen phosphate, 1-2 parts of multi-vitamin and multi-mineral, 0.1-0.3 parts of beta-glucan, 2-5 parts of compound probiotic fermentation agent, and 32-40 parts of water. The compound probiotic fermentation agent is prepared by mixing Haematococcus pluvialis, Bacillus circulans and marine Rhodotorula in a mass ratio of 3-5:3-4:2-3. The fermented feed of the present application can significantly improve the body color, muscle quality and immunity of the crayfish.