The invention relates to the technical field of animal epidemic disease diagnosis, and in particular relates to an enzyme-linked immuno sorbent assay (ELISA) kit for detecting a sheep pseudo rabies virus (PRV) antibody. The ELISA kit comprises an envelop plate, a positive control, a negative control, sample diluent, an enzyme label conjugate, 20*concentration washing liquid, substrate liquid, stop buffer, sealing glue, a self-sealing bag and an operation instruction; the kit is capable of rapidly, sensitively and accurately detecting the PRV antibody in the body of a sheep, so that the blank in the field at home and abroad is made up for. The kit can be used for replacing traditional serological methods such as a virus neutralization test, so that the high-flux and large-scale detection on the PRV antibody in the body of the sheep, with the high sensitivity, strong specificity and good stability, can be realized.