10 results about "Vaccine evaluation" patented technology

This invention relates to the technical field of multi-channel ELISA reaction plates for detecting feline triple vaccine antibodies. It discloses a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies, comprising a reaction plate body, a base plate, and an ELISA reaction plate disposed on top of the base plate. The ELISA reaction plate has an FPV reaction channel, an FCV reaction channel, and an FHV reaction channel disposed on top of the ELISA reaction plate, and an ELISA plate sealing cap is snapped onto the top of the ELISA reaction plate. This invention enables multi-channel parallel reactions in the detection of feline triple vaccine antibodies, saving reagents, improving experimental efficiency, and is suitable for animal experiments, vaccine evaluation, and clinical sample testing. It is also sealable, stackable, and easy to transport.

The invention discloses a construction method and application of a hepatitis D virus replication mouse model. Experiments prove that the generation of HDV RNA and the expression of HDAg protein in mouse liver tissues can be maintained by performing caudal vein high-pressure injection on the pSVL (D3) plasmid. The invention provides a novel mouse model for HDV research, provides a new in-vivo platform for anti-HDV drug screening and vaccine evaluation, and has wide application prospects and market potential.

The invention discloses a porcine rotavirus VP4 protein monoclonal antibody, a kit and application, and belongs to the field of porcine rotavirus antibody detection. The porcine rotavirus VP4 protein monoclonal antibody comprises a heavy chain and a light chain, the amino acid sequence of the heavy chain is shown as SEQ ID NO. 1, and the amino acid sequence of the light chain is shown as SEQ ID NO. 2. According to the present invention, the blocking ELISA detection kit and the detection method are innovatively constructed by using the monoclonal antibody targeting the VP4 conserved functional epitope as the core, and the method can significantly reduce the cross reaction and truly reflect the protective immune level; compared with the prior art, the method provided by the invention has the advantages of no need of species-specific secondary antibody, avoidance of non-specific binding and serum source interference, strong universality, good repeatability and suitability for high-throughput application, fills the technical blank, and provides a novel tool with high specificity, high correlation and high practicability for precise immune monitoring and vaccine evaluation of PoRV.

The invention belongs to the technical field of microbial immunity and DNA vaccines, and particularly relates to a preferred epitope peptide of a Crimean-Congo hemorrhagic fever virus envelope glycoprotein C terminal (CCHFV Gc), a tandem multi-epitope vaccine, an expression vector and application. The preferable epitope peptide is composed of any one amino acid sequence as shown in SEQ ID NO.1-SEQ ID NO.21, and the preferable epitope peptide has high affinity, strong immunogenicity and interspecific amp; and an intraspecific conservative preferred epitope. Preferably epitopes are connected in series through a linker to synthesize a series multi-epitope vaccine, the series multi-epitope vaccine enters cells to be expressed to form recombinant protein, then T lymphocytes are activated, and cellular immune response is induced. The in-vivo vaccine evaluation of animals is carried out by using the tandem multi-epitope vaccine, and it is proved that the vaccine can effectively induce anti-CCHFV specific cellular immune response and the application safety in BALB / c mice. The invention is helpful for understanding the immunobiology of CCHFV Gc and perfecting the epitope vaccine design for the virus in the future.

The invention relates to a type II herpes simplex virus strain HSV-2 / KM-1 and application thereof, and belongs to the technical field of virus microorganisms. The II-type herpes simplex virus strain HSV-2 / KM-1 is preserved in the China Center for Type Culture Collection on July 25, 2025, and the preservation number of the II-type herpes simplex virus strain HSV-2 / KM-1 is CCTCC (China Center for Type Culture Collection) NO: V202550. The II-type herpes simplex virus strain HSV-2 / KM-1 disclosed by the invention has cell tropism and tends to infect genital epithelial cells; the II-type herpes simplex virus strain HSV-2 / KM-1 is applied to neutralizing antibody detection of mouse serum, and the instrumental effect of the II-type herpes simplex virus strain HSV-2 / KM-1 in immunogenicity evaluation of vaccines is disclosed. According to the invention, the II-type herpes simplex virus strain HSV-2 / KM-1 is simultaneously utilized to successfully construct a Hartley guinea pig genital herpes model or a BALB / c mouse genital herpes model, and the models can be used as new tools for HSV-2 vaccine evaluation.

The application discloses a monoclonal antibody of a porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody can specifically recognize a linear B cell epitope on the VP6 protein, and a core sequence of the epitope is TTRLSFQLVR. The antibody has high affinity and high specificity, and can cross-react with porcine rotaviruses of various genotypes such as G5, G9 and G11. The application further includes an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in detection of porcine rotaviruses, development of vaccines and research on functions of viral proteins. The application provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of porcine rotaviruses.

The invention provides a reverse pseudovirus particle based on a receptor as well as a preparation method and application of the reverse pseudovirus particle, and belongs to the technical field of biological pharmacy. A transmembrane protein receptor derived from a host is integrated on an envelope of the pseudovirus particle, and an extracellular structural domain of the transmembrane protein receptor faces the outside of the virus particle, so that the pseudovirus particle can specifically recognize and combine target cells expressing corresponding virus envelope protein, and the pseudovirus particle is mediated to enter the target cells. The skeleton of the pseudovirus particle is selected from one of VSV [delta] G, HIV or MLV. The transmembrane protein receptor is I-type transmembrane protein, and is preferably selected from ACE2, DPP4 or APN. According to the invention, a prefabricated and extensible reverse receptor pseudovirus detection system is constructed, multi-receptor cross-species function evaluation and rapid new coronavirus receptor identification are realized, and an efficient and universal platform is provided for cross-species propagation monitoring, drug screening and vaccine evaluation.

The application discloses a porcine rotavirus VP4 protein monoclonal antibody, a kit and application, and belongs to the field of porcine rotavirus antibody detection.The porcine rotavirus VP4 protein monoclonal antibody comprises a heavy chain and a light chain, the amino acid sequence of the heavy chain is shown as SEQ ID NO.1, and the amino acid sequence of the light chain is shown as SEQ ID NO.2.Innovatively taking the monoclonal antibody targeting a VP4 conservative functional epitope as the core, an ELISA detection kit and a detection method are constructed, the method can significantly reduce cross-reactions, and can truly reflect a protective immune level;without a species-specific secondary antibody, non-specific binding and serum source interference are avoided, the method has strong universality, good repeatability, is suitable for high-throughput application, and fills a technical blank, and provides a new tool with high specificity, high correlation and high practicability for precise immune monitoring and vaccine evaluation of PoRV.

The invention discloses a monoclonal antibody of porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody, the monoclonal antibody can specifically recognize linear B cell epitopes on the VP6 protein, and the epitope core sequence of the monoclonal antibody is TTRLSFQLVR. The antibody has high affinity and high specificity, and can be subjected to cross reaction with various genotypes of porcine rotaviruses such as G5, G9, G11 and the like. The invention also comprises an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in porcine rotavirus detection, vaccine research and development and virus protein function research. The invention provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of the porcine rotavirus.