Building method of EBV (epstein barr virus) guinea pig animal model

An animal model and method establishment technology, applied in the field of medical biology, can solve the problems of the small number of primates, the high price, and the restriction of in-depth research on EBV.

Active Publication Date: 2012-12-12
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The existing animal models of EBV virus are only susceptible to a few primates, and primates are no longer used due to many factors such as small number, high price and maintenance of ecological balance, thus restricting people's understanding of EBV In-depth research, and it is difficult to obtain drugs for the treatment of EBV infection, can not prevent and treat various cancers caused by EBV infection, and many other problems. EBV virus animal model and its establishment method

Method used

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  • Building method of EBV (epstein barr virus) guinea pig animal model
  • Building method of EBV (epstein barr virus) guinea pig animal model
  • Building method of EBV (epstein barr virus) guinea pig animal model

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Comparison scheme
Effect test

Embodiment 1

[0073] 20 guinea pigs were divided into two groups, the experimental group and the control group. The guinea pigs in the experimental group were inoculated with 0.1ml of EB virus liquid by intranasal drip, and the virus titer was 1.0×10 6 ; The guinea pigs of the control group were nasally dripped with 0.1ml PBS.

[0074] Application-specific detection indicators detection.

Embodiment 2

[0076] 20 guinea pigs were divided into two groups, the experimental group and the control group. The guinea pigs in the experimental group were inoculated with 0.1ml of EB virus liquid by intranasal drip, and the virus titer was 5.0×10 6 ; The guinea pigs of the control group were nasally dripped with 0.1ml PBS.

[0077] Application-specific detection indicators detection.

Embodiment 3

[0079] 20 guinea pigs were divided into two groups, the experimental group and the control group. The guinea pigs in the experimental group were inoculated with 0.1ml of EB virus liquid by intranasal drip, and the virus titer was 1.0×10 7 ; The guinea pigs of the control group were nasally dripped with 0.1ml PBS.

[0080]Application-specific detection indicators detection.

[0081] Table 1 shows the changes in the levels of VCA-IgG and EBNA-IgG in the plasma of guinea pigs within 30 weeks after being inoculated with the virus, and the values ​​are OD values. ±s said.

[0082] Table 1

[0083]

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Abstract

The invention provides a building method of an EBV (epstein barr virus) guinea pig animal model. The method comprises the following steps that EBV inoculation is carried out on guinea pigs; the guinea pig peripheral blood lymphocyte separation is carried out; RNA (ribonucleic acid) extraction is carried out; RT-PCR (reverse transcription-polymerase chain reaction) reverse transcription is carried out; Nested-PCR (polymerase chain reaction) amplification is carried out, the targeted DNA (deoxyribonucleic acid) segments are recovered and purified, then, the DNA is connected onto pGEM-T vectors to be subjected to conversion, plasmid extraction and digestion identification, then, the DNA is conveyed to a sequence testing company to test the sequence, the EBV guinea pig animal model is built so that the etiology, the immunology, the pathogenesis and the like of the EBV can be conveniently studied, and the important effect is realized in the application aspects of relevant medicine screening, vaccine evaluation and the like.

Description

[0001] technical field [0002] The invention relates to a method for establishing an animal model, in particular to a method for establishing a guinea pig EB virus animal model, and belongs to the technical field of medical biology. Background technique [0003] The full name of EBV virus is Epstein-Barr virus, which is a virus strain established by Epstein and Barr in 1964 for the first time by successfully using Burkitt African children's lymphoma cells through in vitro suspension culture. EBV is universally infective in the population. Globally, more than 90% of people have been infected by EBV. EBV is mainly transmitted through saliva, but can also be transmitted through blood. Infection mostly occurs in early childhood, usually without or only with weak clinical symptoms. EBV infection can induce a variety of malignant tumors, including Burkitt's lymphoma, gastric cancer, nasopharyngeal carcinoma, and Hodgkin's lymphoma, and is often associated with other viral infec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C12Q1/70C12Q1/68
Inventor 刘国超胡云章胡凝珠王海漩
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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