The invention discloses a 4-tertiary butyl-2-(nitrobenzyl imino) thiazole derivative (I) which has the following chemical structural formula, in the formula I, Xi is selected from hydrogen, methoxy group, trifluoro-methyl, ethyoxyl or chlorine; X2 is selected from hydrogen, methoxy group, trifluoro-methyl, ethyoxyl or chlorine; X3 is selected from hydrogen, methoxy group, trifluoro-methyl, ethyoxyl or chlorine; X4 is selected from hydrogen, methoxy group, trifluoro-methyl, ethyoxyl or chlorine; and NO2 is selected from 2-nitryl, 3-nitryl, 4-nitryl or 2,4-dinitryl. The 4-tertiary butyl-2-(nitrobenzyl imino) thiazole derivative (I) has favorable inhibition activity on human cervical cancer cells, human liver cancer cells, human nasopharyngeal carcinoma cells and the like and can be used for preparing medicaments for resisting tumors.