Kit for detecting 7 genetic markers of peripheral blood in early diagnosis of nasopharyngeal darcinoma

A gene marker and early diagnosis technology, applied in the field of molecular biology, can solve the psychological pressure of EB virus carriers and the inability to screen patients, and achieve high sensitivity and specificity.

Active Publication Date: 2011-01-26
SUN YAT SEN UNIV
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Problems solved by technology

Currently commonly used serological indicators are VCA-IgA and EA-IgA. Clinical laboratories often use indirect enzyme staining to measure serum VCA-IgA and EA-IgA antibody titers for auxiliary diagnosis of nasopharyngeal carcinoma, but there

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  • Kit for detecting 7 genetic markers of peripheral blood in early diagnosis of nasopharyngeal darcinoma
  • Kit for detecting 7 genetic markers of peripheral blood in early diagnosis of nasopharyngeal darcinoma
  • Kit for detecting 7 genetic markers of peripheral blood in early diagnosis of nasopharyngeal darcinoma

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Embodiment Construction

[0064] The present invention is based on the human whole gene expression spectrum chip technology, selects 7 gene markers through the SVM feature selection method and establishes a SVM nasopharyngeal carcinoma diagnostic model composed of these 7 gene markers. The establishment of the model Including the following aspects:

[0065] 1. Agilent human whole gene oligonucleotide chip detection

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Abstract

The invention provides a kit for detecting 7 genetic markers of peripheral blood in early diagnosis of nasopharyngeal darcinoma. The kit comprises a peripheral blood total RNA extracting reagent, RT-PCR reaction liquid, time fluorescence quantitative RT-PCR reaction liquid and an SVM nasopharyngeal darcinoma diagnosis model, wherein the time fluorescence quantitative RT-PCR reaction liquid comprises primers of which the sequences are shown as SEQ IDNO:1-16; the primer sequences are SYBR Green fluorescence quantitative RT-PCR detection primers of the 7 genetic markers and reference gene GAPD; and the 7 genetic markers are HERC5, DLG7, PPARG, PLK1, KIF15, KLHL25 and MYST4. The invention also provides a using method for the kit. The kit has the advantages of high sensitivity, specificity and convenience, and can be used as aided diagnosis and early diagnosis technology for the nasopharyngeal darcinoma.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a detection kit for seven gene markers in peripheral blood used for early diagnosis of nasopharyngeal carcinoma. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern my country and Southeast Asia. It develops progressively and often metastasizes and spreads later. At present, nasopharyngeal carcinoma mainly relies on radiotherapy, and the prognosis of patients with advanced tumors is still very poor. The 5-year survival rate of stage I patients after radiotherapy can reach 90%, and the 5-year survival rate of stage IV patients is only 30%. Therefore, early detection is the key to improving the cure rate. key to rate. Serological screening for high-risk groups of nasopharyngeal carcinoma is an important way to detect early nasopharyngeal carcinoma. Because changes in serological indicators usually precede the appearance of clinical...

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 曾木圣刘万里李满枝洪明晃曹京燕胡利娟张华
Owner SUN YAT SEN UNIV
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